Antioxidant composition containing black mulberry fruit extract and application thereof

Abstract

The invention relates to the field of skin care products, and particularly discloses an antioxidant composition containing black mulberry fruit extract and application of the antioxidant composition. The antioxidant composition containing the black mulberry fruit extract comprises the following components in parts by mass: 10-20 parts of the black mulberry fruit extract; 20-40 parts of a poria cocos extract; 5 to 10 parts of cape jasmine flower extract; 15 to 30 parts of leontopodium alpinum extract; and 50-60 parts of a solvent. The composition disclosed by the invention has the advantage that the anti-oxidation effect is not easily influenced by the storage time and the storage environment. Besides, the antioxidant composition containing the black mulberry fruit extract is applied to skin care products and cosmetics, and the addition amount is 10%-15%, so that the antioxidant effects of the skin care products and the cosmetics added with the antioxidant composition are not easily influenced by the placement time and the placement environment.

Description

Technical Field

[0001] The invention relates to the field of skin care, in particular to an antioxidant composition containing black mulberry fruit extract and application thereof. Background Art

[0002] With the improvement of living standards, people's demand for skin care products is growing. Among them, antioxidant skin care products have attracted widespread attention because of their ability to resist skin aging, neutralize free radicals and reduce damage to skin cells.

[0003] In order to reduce the irritation of antioxidant skin care products to the skin, plant extracts are usually used as antioxidant ingredients in current antioxidant skin care products. However, plant extracts usually have certain activity and stability, and skin care products are usually placed on the dressing table when not in use. Some people even like to put skin care products in the shower room. When the summer sun is bright or the temperature is high, and the shower water temperature is high, resulting in high temperature and humidity in the shower room, the harsh environmental conditions such as ultraviolet rays, high humidity and high temperature may affect the activity and stability of plant extracts, thereby affecting the antioxidant effect of antioxidant skin care products. Therefore, there is still room for improvement. Summary of the invention

[0004] In order to further improve the stability of antioxidant skin care products in a storage environment, the present application provides an antioxidant composition containing black mulberry fruit extract and its application.

[0005] In a first aspect, the present application provides an antioxidant composition containing black mulberry, using the following technical solution: An antioxidant composition containing black mulberry fruit extract comprises the following components in parts by weight: 10-20 parts of black mulberry fruit extract; 20-40 parts of Poria cocos extract; 5-10 parts of Gardenia jasminoides extract; 15-30 parts of alpine edelweiss extract; 50-60 parts of solvent.

[0006] By adopting the above technical scheme, the black mulberry extract is rich in various active substances, such as flavonoids, alkaloids, polyphenols and benzofuran compounds, which can inhibit elastase and have anti-aging effects. At the same time, the compounding of the black mulberry extract with Poria cocos extract, Gardenia jasminoides extract and Leontopodium alpinum extract is also beneficial to further enhance the anti-aging effect of the antioxidant composition, so that the onset time of the anti-aging effect is shorter and the effect is faster.

[0007] In addition, the mutual synergy of black mulberry extract, Poria cocos extract, Gardenia jasminoides extract and alpine edelweiss extract can further improve the stability of the composition system, making the activity of the plant extracts less susceptible to the impact of harsh environments, allowing the composition to maintain sufficient activity after being placed for a long time, and making the anti-aging effect less susceptible to the impact of the environment and placement time, which is beneficial for the composition to always maintain a good anti-aging effect.

[0008] Preferably, the preparation method of the black mulberry fruit extract is as follows: The black mulberry fruit is dried, and then the dried black mulberry fruit is ground into powder, and the black mulberry fruit powder, water and ethanol are mixed in a mass ratio of 1:5-6:5-6, and at the same time, ultrasonic treatment with an ultrasonic power of 300-400W and pulse treatment with a pulse current frequency of 10-15Hz are performed, and extraction is performed at a temperature of 35-45°C for 2-3h to obtain an extract, and the extract is concentrated and powdered to obtain a black mulberry fruit extract.

[0009] By adopting ultrasonic treatment under specific conditions and pulse treatment under specific conditions to synergistically extract black mulberry, the effective ingredients in the black mulberry that can synergize with Poria cocos extract, Gardenia jasminoides extract and Leontopodium alpinum extract can be better extracted, which is beneficial to further enhance the synergistic compound effect of the black mulberry extract with Poria cocos extract, Gardenia jasminoides extract and Leontopodium alpinum extract, so that the anti-aging effect of the composition is further enhanced.

[0010] Preferably, the drying temperature of the black mulberry is 50-60°C.

[0011] By controlling the drying temperature of the black mulberry, the loss of effective components in the black mulberry can be reduced while the drying efficiency of the black mulberry is improved as much as possible, so that the extraction rate and extraction effect of the black mulberry extract are better.

[0012] Preferably, the fineness of the black mulberry powder is 150-250 microns.

[0013] By controlling the fineness of the black mulberry powder, it is helpful to better improve the extraction efficiency, so that the effective ingredients in the black mulberry powder that can synergize with Poria cocos extract, Gardenia jasminoides extract and Leontopodium alpinum extract can be extracted more completely, thereby helping to further enhance the synergistic compound effect of the black mulberry extract with Poria cocos extract, Gardenia jasminoides extract and Leontopodium alpinum extract, so that the anti-aging effect of the composition is further enhanced.

[0014] Preferably, the preparation method of the Poria cocos extract is as follows: Mix Poria cocos and water in a mass ratio of 1:4-6, let stand for 10-12 hours, then raise the temperature to 35-40°C, and at the same time, perform ultrasonic treatment with an ultrasonic power of 500-600W, extract at a constant temperature for 1-1.5 hours to obtain an extract, concentrate the extract, and perform powdering to obtain a Poria cocos extract.

[0015] By using ultrasonic treatment under specific conditions to extract Poria cocos, the effective ingredients in Poria cocos that can synergize with black mulberry extract, gardenia extract and alpine edelweiss extract can be more completely extracted, thereby further enhancing the synergistic compound effect of Poria cocos extract with black mulberry extract, gardenia extract and alpine edelweiss extract, thereby further enhancing the anti-aging effect of the composition.

[0016] Preferably, the preparation method of the gardenia extract is as follows: The gardenia and water are mixed in a mass ratio of 1:6-8, the temperature is increased to 35-40°C, and ultrasonic treatment is performed for 1.5-2h with an ultrasonic power of 450-500W, and the filtrate is filtered to obtain a primary extract for standby use; the filter residue is taken, water with a mass of 2-3 times the mass of the filter residue is added, the pH is adjusted to 3.0-5.0, and 0.5%-1% of papain, 0.3%-0.5% of pectin esterase and 0.1%-0.2% of tannase are added based on the total mass of the filter residue and water, the temperature is increased to 50-55°C, enzymolysis is performed for 30-40min, the enzyme is inactivated, the filter is filtered, the filtrate is taken to obtain a secondary extract, the secondary extract is mixed with the primary extract, and then concentrated and powdered to obtain a gardenia extract.

[0017] By using ultrasonic treatment under specific conditions and adding specific amounts of papain, pectinesterase and tannase to extract gardenia, the effective ingredients in gardenia that can synergize with black mulberry extract, Poria extract and alpine edelweiss extract can be more completely extracted, thereby further enhancing the synergistic compound effect of gardenia extract with black mulberry extract, Poria extract and alpine edelweiss extract, thereby further enhancing the anti-aging effect of the composition.

[0018] Preferably, the preparation method of the alpine edelweiss extract is as follows: The alpine edelweiss is mixed with water in a mass ratio of 1:4-5, the pH is adjusted to 5.0-6.0, and based on the total mass of the alpine edelweiss and water, 1%-1.5% of bromelain, 0.5%-0.8% of polygalacturonase and 0.3%-0.5% of xylanase are added, the temperature is increased to 45-50°C, enzymolysis is performed for 20-30 minutes, the enzyme is inactivated, the filter is filtered, the filtrate is collected, concentrated, and powdered to obtain the alpine edelweiss extract.

[0019] By adding specific amounts of bromelain, polygalacturonase and xylanase to extract alpine edelweiss, the effective ingredients in the alpine edelweiss that can synergize with black mulberry extract, Poria extract and gardenia extract can be more completely extracted, thereby further enhancing the synergistic compound effect of the alpine edelweiss extract with black mulberry extract, Poria extract and gardenia extract, thereby further enhancing the anti-aging effect of the composition.

[0020] In a second aspect, the present application provides an application of an antioxidant composition containing black mulberry fruit extract, using the following technical solution: The invention discloses an application of an antioxidant composition containing a black mulberry fruit extract. The antioxidant composition containing the black mulberry fruit extract is applied to skin care products and cosmetics.

[0021] By adopting the above-mentioned antioxidant composition containing black mulberry extract and applying it in skin care products and cosmetics, it is beneficial to better improve the anti-aging effect of skin care products and cosmetics. At the same time, it is also beneficial to better improve the stability of effective active ingredients, making the anti-aging effect of skin care products and cosmetics less susceptible to the impact of storage time and storage environment.

[0022] Preferably, the antioxidant composition containing black mulberry extract is added in an amount of 10%-15% in skin care products and cosmetics.

[0023] By controlling the amount of antioxidant composition added to skin care products, it is beneficial to maximize the cost-effectiveness of the use of the antioxidant composition, which is beneficial for skin care products and cosmetics to better and more stably exert their anti-aging effects while not easily increasing the cost of skin care products and cosmetics too much.

[0024] In summary, this application has the following beneficial effects: 1. The antioxidant composition formed by compounding black mulberry fruit extract with Poria cocos extract, Gardenia jasminoides extract and Leontopodium alpinum extract is conducive to further enhancing the anti-aging effect, making the onset time of the anti-aging effect shorter and the effect faster; 2. By compounding black mulberry extract with Poria cocos extract, gardenia extract and alpine edelweiss extract to form an antioxidant composition, it is also beneficial to further improve the stability of the composition system, making the activity of the plant extract less susceptible to the impact of harsh environment, so that the composition can still maintain sufficient activity after being placed for a long time, and the anti-aging effect is less susceptible to the impact of the environment and placement time, which is beneficial for the composition to always maintain a good anti-aging effect; 3. By adopting a specific method to extract black mulberry, Poria cocos, gardenia and alpine edelweiss, it is beneficial to better coordinate the effective ingredients of the extracted extracts, so that the anti-aging effect of the composition is better and more stable. DETAILED DESCRIPTION

[0025] The present application is further described in detail below with reference to the embodiments.

[0026] Example 1 The present application example discloses an antioxidant composition containing black mulberry fruit extract, which is composed of the following components: Black mulberry fruit extract 100g; Poria cocos extract 200g; Gardenia jasminoides extract 50g; Alpine edelweiss extract 150g; Solvent 500g.

[0027] In this embodiment, the solvent is deionized water.

[0028] In this embodiment, the preparation method of black mulberry fruit extract is as follows: 10 kg of black mulberry fruit is put into an oven for drying, and the drying temperature is controlled to be 50° C. The dried black mulberry fruit is then ground into powder, and black mulberry fruit powder with a fineness of 150-250 microns is screened out, and 1 kg of the screened black mulberry fruit powder is mixed with 5 kg of water and 5 kg of ethanol, and the temperature is raised to 35° C. At the same time, ultrasonic and pulse treatment are performed, and the ultrasonic power is controlled to be 300 W, and the pulse current frequency is controlled to be 10 Hz. Constant temperature extraction is performed for 3 hours under ultrasonic and pulse conditions, and the filtrate is filtered to obtain an extract. The extract is transferred to a concentrator, and vacuum concentrated at a concentration temperature of 60° C. and a concentration pressure of 0.05 MPa to obtain a concentrated solution, and the concentrated solution is added to a centrifugal spray dryer for drying and powdering, so as to obtain a black mulberry fruit extract.

[0029] In this embodiment, the preparation method of Poria cocos extract is as follows: Take 1 kg of Poria cocos and mix it with 4 kg of water, let it stand for 10 hours, raise the temperature to 35°C, and at the same time, assist with ultrasound and control the ultrasonic power to 500 W. Extract at constant temperature for 1.5 hours under ultrasonic conditions, filter, take the extract, and then add the extract to a centrifugal spray dryer for drying and powdering to obtain Poria cocos extract.

[0030] In this embodiment, the preparation method of Gardenia jasminoides extract is as follows: 1kg of gardenia is mixed with 6kg of water, the temperature is raised to 35°C, ultrasonic wave is used, and the ultrasonic power is controlled to 450W, and the extraction is carried out at constant temperature for 2h under ultrasonic conditions, and the filtrate is taken to obtain a primary extract for standby use. The filter residue is taken, water with a mass twice that of the filter residue is added, stirred evenly, and citric acid is added to adjust the pH to 3.0, and then 0.5% of papain, 0.3% of pectin esterase and 0.1% of tannase are added based on the total mass of the filter residue and water, the temperature is raised to 50°C, and the constant temperature hydrolysis is carried out for 40min, and the temperature is raised to 95°C, the enzyme is inactivated, filtered, and the filtrate is taken to obtain a secondary extract. The secondary extract is mixed with the primary extract, and the mixed extract is added to a centrifugal spray dryer for drying and powdering to obtain a gardenia extract.

[0031] Among them, papain was purchased from Guangdong Mingtong Biotechnology Co., Ltd., CAS No. 9001-73-4; Pectinesterase was purchased from Jinan Shenghe Chemical Co., Ltd. with an EC number of EC3.1.1.11.

[0032] Tanninase was purchased from Hubei Xinghengye Technology Co., Ltd. with a CAS number of 371-41-5.

[0033] In this embodiment, the preparation method of the alpine edelweiss extract is as follows: Take 1 kg of alpine edelweiss and mix it with 4 kg of water, add citric acid to adjust the pH to 5.0, and add 0.05 kg of bromelain, 0.025 kg of polygalacturonase and 0.015 kg of xylanase, raise the temperature to 45 ° C, and perform enzymolysis at a constant temperature for 30 minutes, then raise the temperature to 80 ° C, inactivate the enzyme, filter, take the filtrate to obtain an extract, add the extract to a centrifugal spray dryer for drying and powdering to obtain an alpine edelweiss extract.

[0034] Among them, bromelain was purchased from Jiangsu Weizhirun Biotechnology Co., Ltd. with the product number FDG-2201.

[0035] Polygalacturonase was purchased from Jinan Shenghe Chemical Co., Ltd. with an EC number of EC3.2.1.15.

[0036] Xylanase was purchased from Shanxi Rankang Biotechnology Co., Ltd. with the product number 6-60-78.

[0037] The present application also discloses a method for preparing an antioxidant composition containing black mulberry extract, which is as follows: weigh the black mulberry extract, Poria extract, Gardenia extract and Leontopodium alpinum extract prepared above respectively by mass, mix them, add water, and stir until the above extracts are completely dissolved to obtain an antioxidant composition containing black mulberry extract.

[0038] Example 2 The difference from Example 1 is that the dosage of each component is different, and the preparation process parameters of each component are different, specifically as follows: 200g of black mulberry fruit extract; 400g of Poria cocos extract; 100g of Gardenia jasminoides extract; 300g of Alpine Leontopodium alpinum extract; and 600g of solvent.

[0039] In the preparation of black mulberry extract, the drying temperature of black mulberry is controlled at 60°C, the mass of water and ethanol added to be mixed with black mulberry powder is 6 kg, and after the black mulberry powder is mixed with water and ethanol, the temperature is raised to 45°C, and the ultrasonic power of the constant temperature extraction process is controlled to 400W, the pulse current frequency is controlled to 15Hz, and the extraction time is 2h.

[0040] In the preparation of Poria cocos extract, the mass of water added to mix with Poria cocos is 6 kg, the standing time is 12 hours, and the temperature after standing is increased to 40°C. The ultrasonic power of the constant temperature extraction process is controlled to 600 W, and the extraction time is 1 hour.

[0041] In the preparation of the gardenia extract, the mass of water added to the gardenia is 8 kg, and after the gardenia is mixed with the water, the temperature is raised to 40°C, and the ultrasonic power of the constant temperature extraction process is controlled to be 500 W, and the ultrasonic treatment time is controlled to be 1.5 hours. After filtration, the filter residue is taken and water with a mass of 3 times the mass of the filter residue is added, and then citric acid is added to adjust the pH to 5.0, and based on the total mass of the filter residue and water, the addition amount of papain is 1%, the addition amount of pectin esterase is 0.5%, and the addition amount of tannase is 0.2%, and after the enzyme is added, the temperature is raised to 55°C, and the enzymolysis time is controlled to be 30 minutes.

[0042] In the preparation of the alpine edelweiss extract, the mass of water added to be mixed with the alpine edelweiss is 5 kg, and citric acid is added to adjust the pH to 6.0, and the amount of bromelain added is 0.09 kg, the amount of polygalacturonase added is 0.048 kg, and the amount of xylanase added is 0.03 kg. After the enzyme is added, the temperature is raised to 50°C and the enzymolysis time is controlled to 20 minutes.

[0043] Example 3 The difference from Example 2 is: Black mulberry fruit extract, Poria cocos extract, Gardenia jasminoides extract and Leontopodium alpinum extract are all commercially available products. Specifically: Black mulberry fruit extract was purchased from Baoji Liupanyun Biotechnology Co., Ltd., extracted with water, and the extraction ratio was 10:1; Poria cocos extract was purchased from Baoji Cosmeco Cosmetics Raw Material Development Co., Ltd., extracted with water, and the extraction ratio was 10:1; Gardenia extract was purchased from Baoji Cosmeco Cosmetics Raw Material Development Co., Ltd., extracted with water, and the extraction ratio was 10:1; The alpine edelweiss extract was purchased from Lanzhou Waterless Biotechnology Co., Ltd., extracted with water, and the extraction ratio was 10:1.

[0044] Example 4 The difference from Example 2 is that the preparation process parameters of each component are different, as follows: In the preparation of the black mulberry fruit extract, the ultrasonic power of the constant temperature extraction process was controlled to be 250W, and the pulse current frequency was controlled to be 5Hz.

[0045] In the preparation of Poria cocos extract, the ultrasonic power of the constant temperature extraction process was controlled to 450W.

[0046] In the preparation of gardenia extract, the ultrasonic power of the constant temperature extraction process of the extract is controlled to be 400W; in the enzymatic hydrolysis of the filter residue, an equal amount of bromelain is used to replace papain, an equal amount of pectin lyase is used to replace pectinesterase, and an equal amount of polyphenol oxidase is used to replace tannase, and the enzymatic hydrolysis temperature is controlled to be 50°C, and the enzyme inactivation temperature is controlled to be 100°C.

[0047] In the preparation of the alpine edelweiss extract, an equal amount of papain is used to replace bromelain, an equal amount of pectin lyase is used to replace polygalacturonase, an equal amount of β-glucanase is used to replace xylanase, and the enzymatic hydrolysis temperature is controlled at 45°C and the enzyme inactivation temperature is controlled at 100°C.

[0048] Among them, bromelain was purchased from Jiangsu Weizhirun Biotechnology Co., Ltd. with the product number FDG-2201.

[0049] Pectin lyase was purchased from Cangzhou Xiasheng Enzyme Biotechnology Co., Ltd. with the product number FDY-2211.

[0050] Polyphenol oxidase was purchased from Zhejiang Yicun Biotechnology Co., Ltd. with a CAS number of 80498-15-3.

[0051] Papain was purchased from Guangdong Mingtong Biotechnology Co., Ltd. with a CAS number of 9001-73-4.

[0052] β-Glucanase was purchased from Jiangsu Runfeng Synthetic Technology Co., Ltd. with CAS number 9025-70-1.

[0053] Example 5 The difference from Example 2 is that the preparation process parameters of each component are different, as follows: In the preparation of the black mulberry fruit extract, the ultrasonic power of the constant temperature extraction process was controlled to be 450W, and the pulse current frequency was controlled to be 20Hz.

[0054] In the preparation of Poria cocos extract, the ultrasonic power of the constant temperature extraction process was controlled to 450W.

[0055] In the preparation of gardenia extract, the ultrasonic power of the constant temperature extraction process of the extract is controlled to be 650W; in the enzymatic hydrolysis of the filter residue, an equal amount of bromelain is used to replace papain, an equal amount of pectin lyase is used to replace pectinesterase, and an equal amount of polyphenol oxidase is used to replace tannase, and the enzymatic hydrolysis temperature is controlled to be 50°C, and the enzyme inactivation temperature is controlled to be 100°C.

[0056] In the preparation of the alpine edelweiss extract, an equal amount of papain is used to replace bromelain, an equal amount of pectin lyase is used to replace polygalacturonase, an equal amount of β-glucanase is used to replace xylanase, and the enzymatic hydrolysis temperature is controlled at 45°C and the enzyme inactivation temperature is controlled at 100°C.

[0057] Among them, bromelain was purchased from Jiangsu Weizhirun Biotechnology Co., Ltd. with the product number FDG-2201.

[0058] Pectin lyase was purchased from Cangzhou Xiasheng Enzyme Biotechnology Co., Ltd. with the product number FDY-2211.

[0059] Polyphenol oxidase was purchased from Zhejiang Yicun Biotechnology Co., Ltd. with a CAS number of 80498-15-3.

[0060] Papain was purchased from Guangdong Mingtong Biotechnology Co., Ltd. with a CAS number of 9001-73-4.

[0061] β-Glucanase was purchased from Jiangsu Runfeng Synthetic Technology Co., Ltd. with CAS number 9025-70-1.

[0062] Comparative Example 1 The difference from Example 3 is that the black mulberry extract is replaced by an equal amount of blackberry extract.

[0063] Among them, blackberry extract was purchased from Xi'an Anao Biotechnology Co., Ltd., extracted with water, and the extraction ratio was 10:1.

[0064] Comparative Example 2 The difference from Example 3 is that an equal amount of Versicolor extract is used to replace the Poria extract.

[0065] Among them, the Versicolor extract was purchased from Shaanxi Junhe Biotechnology Co., Ltd., extracted with water, and the extraction ratio was 10:1.

[0066] Comparative Example 3 The difference from Example 3 is that the Gardenia jasminoides extract is replaced by an equal amount of Honeysuckle Flos extract.

[0067] Among them, the honeysuckle extract was purchased from Nanjing Taigao Plant Extract Biotechnology Co., Ltd., extracted with water, and the extraction ratio was 10:1.

[0068] Comparative Example 4 The difference from Example 3 is that the alpine edelweiss extract is replaced by an equal amount of Rhodiola rosea extract.

[0069] Among them, Rhodiola rosea extract was purchased from Shaanxi Huinengda Biotechnology Co., Ltd. with the product number h8482641.

[0070] Comparative Example 5 The difference from Example 3 is that an equal amount of blackberry extract is used to replace black mulberry extract; an equal amount of Versicolor extract is used to replace Poria extract; an equal amount of honeysuckle extract is used to replace Gardenia extract; and an equal amount of Rhodiola rosea extract is used to replace Leontopodium alpinum extract.

[0071] Among them, blackberry extract was purchased from Xi'an Anao Biotechnology Co., Ltd., extracted with water, and the extraction ratio was 10:1; Versicolor extract was purchased from Shaanxi Junhe Biotechnology Co., Ltd., extracted with water, and the extraction ratio was 10:1; Honeysuckle extract was purchased from Nanjing Taigao Plant Extract Biotechnology Co., Ltd., extracted with water, and the extraction ratio was 10:1; Rhodiola rosea extract was purchased from Shaanxi Huinengda Biotechnology Co., Ltd. with the product number h8482641.

[0072] Experiment 1 20 mL of the antioxidant composition prepared in the above examples and comparative examples were respectively placed in a beaker, and then 2 mL of the antioxidant composition was taken from the beaker and added to a colorimetric tube, and then 4 mL of a DPPH free radical solution with a mass concentration of 0.05 mg / mL was added to the colorimetric tube, and the volume was made up to 100 mL with ultrapure water, and then placed in a dark place to avoid light for 50 minutes, and then the absorbance was measured at 517 nm using an ultraviolet spectrophotometer, recorded as A2, and 0.5 mg / mL of vitamin C solution was used as a positive control, recorded as A1, and the initial free radical scavenging rate (%) was calculated according to the following formula:

[0073] The beaker and the remaining antioxidant composition in the beaker were placed in a thermostat at a temperature of 50°C and a humidity of 70% for 30 minutes, and then irradiated with a UVB lamp for 20 minutes, and the UVB irradiation dose was controlled to be 80 mJ / cm 2After the treatment is completed, 2 mL of the antioxidant composition is taken from the beaker and added to the colorimetric tube, and then 4 mL of DPPH free radical solution with a mass concentration of 0.05 mg / mL is added to the colorimetric tube, and the volume is made up to 100 mL with ultrapure water, and then placed in a dark place to avoid light for 50 minutes, and then the absorbance is measured at 517 nm using an ultraviolet spectrophotometer, recorded as A4, and 0.5 mg / mL vitamin C solution is used as a positive control, recorded as A3, and the free radical scavenging rate (%) after treatment is calculated according to the following formula:

[0074] The above calculated data were recorded, and then the change rate (%) of the free radical scavenging rate of the antioxidant composition before and after high temperature and ultraviolet light irradiation treatment was calculated according to the following formula:

[0075] The test data of the above experiment 1 are detailed in Table 1.

[0076] Experiment 2 100 mL of the antioxidant composition prepared in the above examples and comparative examples were taken respectively, and then 900 mL of deionized water was added to dilute them to prepare test samples for later use.

[0077] 100 female volunteers aged 45-50 with similar skin conditions were randomly selected and divided into 10 groups, with 10 people in each group. The skin firmness of the volunteers' facial skin was first tested using the skin elasticity meter Corneometer MP A580, and the measurement value of each volunteer was recorded and the average value of the measurement values ​​of the volunteers in the same group was calculated, which was recorded as the skin firmness before using the product; and the skin wrinkles of the volunteers' facial skin were tested using the skin fast three-dimensional imaging system PRIMOS, and the measurement value of each volunteer was recorded and the average value of the measurement values ​​of the volunteers in the same group was calculated, which was recorded as the skin wrinkles before using the product. Among them, the smaller the measurement value, the better the skin firmness and the fewer wrinkles, that is, the better the antioxidant effect.

[0078] After the above tests were completed, the volunteers began to use the test samples prepared by using the antioxidant compositions of the above embodiments and comparative examples respectively, and after 30 days, the skin was tested again, as follows: After cleansing their faces in the morning and evening, volunteers took 1 mL of the test sample prepared above and applied it evenly on their faces. They persisted in using it for 30 days. During the experiment, the volunteers were placed in a room with a room temperature of 27°C and sat quietly. During the experiment, the volunteers temporarily stopped using other skin care products or makeup products. After cleansing their faces on the morning of the 31st day, the skin firmness of the volunteers' facial skin was re-tested using the skin elasticity meter Corneometer MP A580, and the measurement value of each volunteer was recorded and the average measurement value of the volunteers in the same group was calculated, which was recorded as the skin firmness after using the product; and the skin wrinkles of the volunteers' facial skin were re-tested using the skin fast three-dimensional imaging system PRIMOS, and the measurement value of each volunteer was recorded and the average measurement value of the volunteers in the same group was calculated, which was recorded as the skin wrinkles after using the product. Among them, the smaller the measurement value, the better the skin firmness and the fewer wrinkles, that is, the better the antioxidant effect.

[0079] The test data of the above experiment 2 is detailed in Table 2.

[0080] Table 1 Table 2 According to the data comparison between Example 3 and Comparative Examples 1-5 in Table 1 and Table 2, Example 3 uses black mulberry fruit extract, Poria cocos extract, Gardenia jasminoides extract and alpine edelweiss extract for compounding, while Comparative Examples 1-4 respectively replace the above ingredients with other antioxidant ingredients, and Comparative Example 5 replaces all the above ingredients with other antioxidant ingredients. Although the free radical scavenging effects of Comparative Examples 1-5 before high temperature and ultraviolet light irradiation treatment are similar to those of Example 3, the free radical scavenging effects of Comparative Examples 1-5 after high temperature and ultraviolet light irradiation treatment are far worse than those of Example 3, and the changes in free radical scavenging rates of Comparative Examples 1-5 before and after treatment are also much greater than those of Example 3, which proves that only by using black mulberry fruit extract, Poria cocos extract, Gardenia jasminoides extract and alpine edelweiss extract in synergy with each other can the stability of the antioxidant composition be further improved, so that the anti-aging effect of the antioxidant composition is less susceptible to the influence of storage time and environment.

[0081] According to the data comparison between Example 1 and Examples 3-5 in Table 1 and Table 2, the black mulberry extract, Poria cocos extract, Gardenia jasminoides extract and Leontopodium alpinum extract in Example 1 were all extracted by a specific extraction method, while Example 3 was compounded with ingredients directly purchased from the market, and Examples 4-5 changed the process parameters and enzyme types of the above extracts during the extraction process. Although the free radical scavenging rates of Examples 3-5 can reach a high level, only the antioxidant composition of Example 1 has the effect of further improving skin firmness and eliminating wrinkles after use, while Examples 3-5 only have the effect of delaying skin sagging and delaying the formation of skin wrinkles. This proves that only by extracting black mulberry, Poria cocos, Gardenia jasminoides and Leontopodium alpinum under specific conditions can the effective ingredients of the extracted extracts be better synergistically compounded to achieve an anti-aging effect.

[0082] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make modifications to the present embodiment without any creative contribution as needed, but such modifications are protected by the patent law as long as they are within the scope of the claims of the present application.

Claims

1. An antioxidant composition containing black mulberry fruit extract, characterized in that: The composition includes the following components in parts by weight: 10-20 parts of black mulberry fruit extract; 20-40 parts of Poria cocos extract; 5-10 parts of Gardenia jasminoides extract; 15-30 parts of alpine edelweiss extract; 50-60 parts of solvent.

2. The antioxidant composition containing black mulberry fruit extract according to claim 1, characterized in that: The preparation method of the black mulberry fruit extract is as follows: The black mulberry fruit is dried, and then the dried black mulberry fruit is ground into powder, and the black mulberry fruit powder, water and ethanol are mixed in a mass ratio of 1:5-6:5-6, and at the same time, ultrasonic treatment with an ultrasonic power of 300-400W and pulse treatment with a pulse current frequency of 10-15Hz are performed, and extraction is performed at a temperature of 35-45°C for 2-3h to obtain an extract, and the extract is concentrated and powdered to obtain a black mulberry fruit extract.

3. The antioxidant composition containing black mulberry extract according to claim 2, characterized in that: The drying temperature of the black mulberry is 50-60°C.

4. The antioxidant composition containing black mulberry fruit extract according to claim 2, characterized in that: The fineness of the black mulberry powder is 150-250 microns.

5. The antioxidant composition containing black mulberry fruit extract according to claim 1, characterized in that: The preparation method of the Poria cocos extract is as follows: Mix Poria cocos and water in a mass ratio of 1:4-6, let stand for 10-12 hours, then raise the temperature to 40-45°C, and simultaneously, perform ultrasonic treatment with an ultrasonic power of 500-600W, extract at a constant temperature for 1-1.5 hours to obtain an extract, concentrate the extract, and perform powdering to obtain a Poria cocos extract.

6. The antioxidant composition containing black mulberry fruit extract according to claim 1, characterized in that: The preparation method of the gardenia extract is as follows: The gardenia and water are mixed in a mass ratio of 1:6-8, the temperature is increased to 35-40°C, and ultrasonic treatment is carried out for 1.5-2h with an ultrasonic power of 450-500W, and the filtrate is filtered to obtain a primary extract for standby use; the filter residue is taken, water with a mass of 2-3 times the mass of the filter residue is added, the pH is adjusted to 3.0-5.0, and 0.5%-1% of papain, 0.3%-0.5% of pectinesterase and 0.1%-0.2% of tannase are added based on the total mass of the filter residue and water, the temperature is increased to 50-55°C, enzymolysis is carried out for 30-40min, the enzyme is inactivated, the filter is filtered, the filtrate is taken to obtain a secondary extract, the secondary extract is mixed with the primary extract, and then concentrated and powdered to obtain the gardenia extract.

7. The antioxidant composition containing black mulberry fruit extract according to claim 1, characterized in that: The preparation method of the alpine edelweiss extract is as follows: Mix alpine edelweiss and water in a mass ratio of 1:4-5, adjust the pH to 5.0-6.0, and add 1%-1.5% bromelain, 0.5%-0.8% polygalacturonase and 0.3%-0.5% xylanase based on the total mass of alpine edelweiss and water, increase the temperature to 45-50°C, perform enzymolysis for 20-30 minutes, inactivate the enzyme, filter, collect the filtrate, concentrate and spray powder to obtain the alpine edelweiss extract.

8. Use of an antioxidant composition containing black mulberry fruit extract according to any one of claims 1 to 7, characterized in that: The antioxidant composition containing the black mulberry fruit extract is applied to skin care products and cosmetics.

9. The use of an antioxidant composition containing black mulberry extract according to claim 8, characterized in that: The antioxidant composition containing the black mulberry fruit extract is added in an amount of 10%-15% in skin care products and cosmetics.

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