Agricultural composition for preventing and treating populus euphratica

Abstract

The invention belongs to the technical field of agricultural compositions, and relates to an agricultural composition for preventing and treating populus euphratica. The invention provides an agricultural composition for preventing and treating populus euphratica, the effective components of the agricultural composition comprise flupyradifurone and echinacoside, and the mass ratio of flupyradifurone to echinacoside is 1: (1-37.5). Insecticidal effect verification finds that echinacoside has a synergistic effect on the chemical insecticidal component flupyradifurone, and the echinacoside and the flupyradifurone are combined, so that the dosage of effective components of chemical pesticides can be effectively reduced, the pesticide effect of the flupyradifurone can be fully exerted, the probability of drug resistance of the populus euphratica can be reduced as much as possible, the environmental pollution is reduced, and the effect of the flupyradifurone is improved.

Description

Technical Field

[0001] The invention belongs to the technical field of agricultural compositions and relates to an agricultural composition for preventing and controlling poplar psyllids. Background Art

[0002] The eggs of the poplar psyllid are mainly laid on young leaves. After the eggs hatch, the first-instar nymphs crawl to the tender leaves at the top of the branches and stop moving, start feeding, and stimulate the host tissue to sag and proliferate. The proliferating part gradually wraps the insect body in the leaf to form galls, one insect per gall. When the insect population is large, the front and back of the leaf are covered with galls, causing the leaves to wither and fall off, seriously affecting the growth of poplar wood and restricting the development of the poplar wood industry.

[0003] Flupyrone is a butenolide compound and a nicotinic acetylcholine receptor agonist. It is classified as a nicotinic acetylcholine receptor modulator Group 4D subgroup by the International Insecticide Resistance Action Committee (IRAC). Flupyrone can selectively act on the insect central nervous system, bind to receptor proteins, and then activate the receptor to produce biological reactions, making the nerve cells in an excited state. However, flupyrone will not be inactivated by acetylcholinesterase, so the receptor remains open, causing the insect nervous system to collapse. Flupyrone contains a special pharmacological group butenolide, which has good systemic conductivity. After foliar spraying or seed treatment, it can be quickly transmitted to various parts of the plant, which can effectively prevent and control pests that feed on the back of the leaves or in hiding, and can reduce the transmission of pathogens that rely on insects by inhibiting insect feeding. Flupyrone, as a broad-spectrum insecticide, can also be used to control poplar psyllids.

[0004] However, during the growth of Populus euphratica, long-term application of a single chemical pesticide can easily cause pests to develop resistance. As pest resistance accumulates, the control effect of chemical pesticides gradually decreases, or even becomes ineffective. Reasonable compounding of active ingredients is one of the effective and convenient means to develop pesticides and control resistant pests.

[0005] CN118077707A discloses an insecticide composition for controlling olive star room psyllid, wherein the active ingredient is a compound of thiofluoxime and bifenazate, fluazinam or flupyralidone, and the mass ratio of thiofluoxime to bifenazate is 1-40:25-1. The insecticide composition has a synergistic effect on olive star room psyllid.

[0006] CN111436441A discloses a pesticide composition for controlling citrus psyllids, the pesticide composition comprising: a first active ingredient, flupyrazone, a second active ingredient, amitraz, and a mass ratio of flupyrazone to amitraz of 1:2 to 1:3.5. The pesticide composition is effective in controlling citrus psyllids by utilizing the complementary effects of flupyrazone and amitraz in terms of efficacy.

[0007] The above patents all use known chemical pesticide ingredients in combination with fluopyrazone to improve the efficacy for the control of psyllids. Long-term use still poses environmental pollution and potential resistance problems. Finding other environmentally friendly and non-resistant ingredients to be combined with fluopyrazone to control poplar psyllids has become a current problem to be solved.

[0008] Echinacoside (ECH) is a phenylethanol glycoside compound with pharmacological effects such as neuroprotection, myocardial protection, liver protection, anti-tumor, anti-oxidation and anti-aging, immunomodulation, hypoglycemic and lipid-lowering, promotion of reproduction and bone formation, protection of pulmonary blood vessels, and prevention of atherosclerosis. Echinacoside is environmentally friendly, there is no report of pest resistance, and there is no literature report on the application of echinacoside and pesticide ingredients in the prevention and control of poplar psyllids. Ordinary technicians in this field cannot know whether echinacoside can be compounded with existing pesticide ingredients to produce synergistic or antagonistic effects without experiments. The applicant found through experimental research that after echinacoside is mixed with flupyfuranone in a certain proportion, the efficiency of the insecticide for poplar psyllids is significantly improved. In addition, the dosage of the insecticide can be reduced to reduce environmental pollution. At present, the mechanism of the insecticide synergistic effect of echinacoside is unclear, and it has not been used in the control of lice on plants. Summary of the invention

[0009] To achieve the above object, the present invention aims to provide an agricultural composition for controlling poplar psyllids by enhancing the synergistic effect of flupyfuranone with echinacoside, wherein the active ingredients of the agricultural composition are composed of echinacoside and flupyfuranone, and the mixing mass ratio of flupyfuranone to echinacoside is 1:1 to 37.5. The present invention uses echinacoside, which has no identical or similar mechanism of action to flupyfuranone, to mix with it to obtain a composition, which can achieve synergistic effect, effectively improve the insecticidal effect, reduce and delay the development of resistance, reduce the amount of chemical insecticide flupyfuranone, and reduce environmental pollution.

[0010] On the one hand, the present invention relates to an agricultural composition for preventing and controlling Populus euphratica, wherein the active ingredients of the agricultural composition are composed of echinacoside and flupyrazone, and the mass ratio of flupyrazone to echinacoside is 1:1-37.5.

[0011] Preferably, in the agricultural composition for controlling Populus euphratica provided by the present invention, the mass ratio of flupyrazone to echinacoside is 1:10 to 32.5.

[0012] More preferably, in the agricultural composition for controlling Populus euphratica provided by the present invention, the mass ratio of flupyrazone to echinacoside is 1:22.5-30.

[0013] More preferably, in the agricultural composition for controlling Populus euphratica provided by the present invention, the mass ratio of flupyrazone to echinacoside is 1:27.5.

[0014] On the other hand, the present invention relates to a use of an agricultural composition for controlling poplar psyllid, comprising: allowing the agricultural composition for controlling poplar psyllid to act on poplar psyllid and / or their hosts.

[0015] Furthermore, for the convenience of field use, the pesticide containing the agricultural composition for controlling poplar psyllids is prepared as a suspension, an aqueous emulsion or an emulsifiable concentrate.

[0016] The above-mentioned dosage form also contains adjuvants required for preparing pesticide preparations, and the adjuvants may be one or a mixture of solvents, emulsifiers, wetting agents, stabilizers, dispersants, thickeners, pH regulators, defoamers, antifreeze agents, fillers, etc. The adjuvants described in the present invention may all be known substances, such as various adjuvants commonly used in pesticide preparations, and may vary according to different situations, and are not particularly limited.

[0017] Compared with the prior art, the technical solution provided by the present invention has at least the following beneficial effects or advantages:

[0018] The present invention applies echinacoside, an environmentally friendly raw material, to the prevention and control of poplar psyllids for the first time, and combines echinacoside with a chemical insecticide, flupyfuranone, for the first time. It is found that echinacoside has a synergistic effect on flupyfuranone, and that after flupyfuranone and echinacoside are mixed at a ratio of 1:27.5, the activity coefficient reaches 2.63, and the field insecticide efficiency is as high as 93.36%, indicating that the use of echinacoside as a synergist in combination with flupyfuranone can greatly reduce the dosage of the active ingredient of the chemical pesticide, give full play to the efficacy of flupyfuranone, slow down the probability of pests developing drug resistance as much as possible, and reduce environmental pollution caused by long-term use. DETAILED DESCRIPTION

[0019] The technical scheme of the present invention is described below in conjunction with the embodiments, but the present invention is not limited to the following embodiments. The experimental methods and detection methods described in each embodiment are conventional methods unless otherwise specified; the reagents and materials described are all commercially available unless otherwise specified.

[0020] Echinacoside: purchased from Nanjing Tianrun Biotechnology Co., Ltd., with a purity of 90%, and was in powder form after passing through an 80-mesh sieve.

[0021] Fluoropyranone: purchased from Shaanxi Didu Pharmaceutical Chemical Co., Ltd., with a purity specification of 98%.

[0022] Example 1

[0023] This example is a test on the toxicity of a mixture of flupyrazone and echinacoside to Populus euphratica.

[0024] Indoor toxicity determination: Toxicity determination of echinacoside enhancing flupyrazone in controlling poplar psyllid.

[0025] Test insect: 1st instar adult of Populus euphratica.

[0026] Treatment of test agents: Dissolve the test agents with dimethyl sulfoxide first, and then dilute with 0.1% Tween-80 aqueous solution to prepare single-dose stock solutions and mixed stock solutions of various proportions. Set different mass concentration gradients for both single-dose and mixed solutions for later use.

[0027] Experimental method: Place 1st instar nymphs on fresh Populus euphratica leaves, 10 nymphs per leaf (50 per group, repeated 3 times), spray the test agent in the order of concentration from low to high, and spray water as a blank control. Spray the liquid evenly on the leaf surface, wrap the petiole with soaked absorbent cotton after natural air drying, put it in a culture dish, count the number of nymph deaths, check the mortality rate after 24 hours, and the death standard is no reaction when the insect body is touched with a brush tip, and the blank control mortality rate is less than 10% for a valid test.

[0028] Data analysis: Regression analysis was performed based on the logarithmic values ​​of each drug concentration and the corresponding mortality probability values ​​to calculate the LC of each drug. 50 The activity coefficient was calculated and the test results are shown in Table 1.

[0029] Activity coefficient = (LC of flupyrazone 50 ) / (LC of flupyrazone and echinacoside compounded 50 )

[0030] The criteria for determining whether there is synergistic effect are: activity coefficient = 1 indicates no synergistic effect, activity coefficient > 1 indicates synergistic effect, and activity coefficient < 1 indicates antagonistic effect.

[0031] The toxicity regression curve of flupyrazone to Populus euphratica obtained by the above test is: y=1.5399x-3.1147, the correlation coefficient is 0.996, and the LC50 is 105.36 mg / L. When the test concentration of echinacoside is increased to 300 mg / L, there is still a survival rate of 85.82% (survival rate=1-mortality rate), indicating that echinacoside has no obvious toxic effect on the first-instar nymphs of Populus euphratica.

[0032] Table 1: Toxicity test results of flupyrazone and echinacoside mixture to Populus euphratica

[0033] Flupyralidone 105.35 / Flupyralidone:echinacoside=1:0.1 157.24 0.67 Flupyralidone:echinacoside=1:0.5 133.35 0.79 Flupyralidone:echinacoside=1:1 89.28 1.18 Flupyralidone:echinacoside=1:5 74.19 1.42 Flupyralidone:echinacoside=1:10 63.85 1.65 Flupyralidone:echinacoside=1:15 55.74 1.89 Flupyralidone:echinacoside=1:20 50.89 2.07 Flupyralidone:echinacoside=1:22.5 47.89 2.20 Flupyralidone:echinacoside=1:25 44.83 2.35 Flupyralidone:echinacoside=1:27.5 40.06 2.63 Flupyralidone:echinacoside=1:30 45.61 2.31 Flupyralidone:echinacoside=1:31.75 54.03 1.95 Flupyralidone:echinacoside=1:32.5 67.97 1.55 Flupyralidone:echinacoside=1:35 79.21 1.33 Flupyralidone:echinacoside=1:37.5 90.04 1.17 Flupyralidone:echinacoside=1:40 150.50 0.70 Flupyralidone:echinacoside=1:50 191.55 0.55 Flupyralidone:echinacoside=1:60 210.70 0.50

[0034] The results of the toxicity test of the mixture of fluoropyrafurone and echinacoside on poplar psyllids are shown in Table 1. After the mixture of fluoropyrafurone and echinacoside, within the ratio range provided by the present invention, the activity coefficient is greater than 1 when the mass ratio is 1:1 to 37.5, and the synergistic effect of echinacoside on the insecticidal effect of fluoropyrafurone is very significant. In particular, when the mixture of fluoropyrafurone and echinacoside is 1:22.5 to 30, the activity coefficient reaches more than 2.2; when the mixture of fluoropyrafurone and echinacoside is 1:27.5, the activity coefficient reaches 2.63, and the effect is the best. The above results show that the use of echinacoside as a synergist in combination with fluoropyrafurone can greatly reduce the dosage of the active ingredient of the pesticide, give full play to the efficacy of fluoropyrafurone, slow down the probability of pests developing resistance as much as possible, and improve the control effect of fluoropyrafurone on poplar psyllids, showing a synergistic effect.

[0035] Preparation Example 1

[0036] This embodiment provides an agricultural composition aqueous emulsion for controlling poplar psyllids.

[0037] Weigh 1 part of flupyrrolidone, 1 part of echinacoside, 5 parts of trimethylbenzene, 5 parts of cyclohexanone, 8 parts of castor oil polyoxyethylene ether, 5 parts of glycerol fatty acid ester polyoxyethylene ether, 1 part of sodium citrate, 2 parts of glycerol, and 42 parts of deionized water according to the mass fraction. The above active ingredients, solvent, emulsifier, dispersant and stabilizer are mixed to form an oil phase, the antifreeze is dissolved in water to form an aqueous phase, the oil phase is slowly added to the aqueous phase under the action of a shearing machine, and the shearing is continued for about 30 minutes to prepare an agricultural composition aqueous emulsion for controlling poplar psyllids.

[0038] Preparation Example 2

[0039] This embodiment provides an agricultural composition emulsifiable concentrate for controlling poplar psyllids.

[0040] Weigh 1 part of flupyrazone, 10 parts of echinacoside, 5 parts of trimethylbenzene, 5 parts of cyclohexanone, 10 parts of polyoxyethylene fatty alcohol ether, 2 parts of doudou, and 40 parts of rosin-based vegetable oil according to mass fractions. Add the above active ingredients into the solvent and fully dissolve them, then add the emulsifier and synergist and stir evenly to form a uniform and transparent oily liquid, and the agricultural composition emulsifiable concentrate for preventing and controlling poplar psyllids can be prepared.

[0041] Preparation Example 3

[0042] This embodiment provides an agricultural composition aqueous emulsion for controlling poplar psyllids.

[0043] Weigh 1 part of flupyrrolidone, 20 parts of echinacoside, 5 parts of trimethylbenzene, 5 parts of cyclohexanone, 10 parts of castor oil polyoxyethylene ether, 5 parts of glycerol fatty acid ester polyoxyethylene ether, 1 part of sodium citrate, 2 parts of glycerol, and 30 parts of deionized water according to the mass fraction. The above active ingredients, solvent, emulsifier, dispersant and stabilizer are mixed to form an oil phase, the antifreeze is dissolved in water to form an aqueous phase, the oil phase is slowly added to the aqueous phase under the action of a shearing machine, and the shearing is continued for about 30 minutes to prepare an agricultural composition aqueous emulsion for controlling poplar psyllids.

[0044] Preparation Example 4

[0045] This embodiment provides an agricultural composition suspension for controlling Populus euphratica.

[0046] Weigh 10 parts of flupyrone, 225 parts of echinacoside, 60 parts of sodium dodecyl sulfonate, 50 parts of sodium methylene bisnaphthalene sulfonate, 30 parts of polycarboxylate, 50 parts of propylene glycol, 10 parts of carboxymethyl cellulose, 10 parts of silicone oil, and 250 parts of deionized water according to mass fractions. The above active ingredients, wetting agent, dispersant, antifreeze agent, thickener and defoamer are dispersed by high-speed shearing and sand-milled in a sand mill to form a highly dispersed and stable suspension system in an aqueous medium, thereby preparing an agricultural composition suspension for controlling poplar psyllids.

[0047] Preparation Example 5

[0048] This embodiment provides an agricultural composition suspension for controlling Populus euphratica.

[0049] Weigh 10 parts of flupyrone, 275 parts of echinacoside, 60 parts of sodium dodecyl sulfonate, 40 parts of sodium methylene bisnaphthalene sulfonate, 30 parts of polycarboxylate, 50 parts of propylene glycol, 10 parts of carboxymethyl cellulose, 10 parts of silicone oil, and 200 parts of deionized water according to mass fractions. The above active ingredients, wetting agent, dispersant, antifreeze agent, thickener and defoamer are dispersed by high-speed shearing and sand-milled in a sand mill to form a highly dispersed and stable suspension system in an aqueous medium, thereby preparing an agricultural composition suspension for controlling poplar psyllids.

[0050] Preparation Example 6

[0051] This embodiment provides an agricultural composition suspension for controlling Populus euphratica.

[0052] Weigh 1 part of flupyrone, 30 parts of echinacoside, 6 parts of sodium dodecyl sulfonate, 5 parts of sodium methylene bisnaphthalene sulfonate, 3 parts of polycarboxylate, 8 parts of propylene glycol, 1 part of carboxymethyl cellulose, 1 part of silicone oil, and 20 parts of deionized water according to mass fractions. The above active ingredients, wetting agent, dispersant, antifreeze agent, thickener and defoamer are dispersed by high-speed shearing and sand-milled in a sand mill to form a highly dispersed and stable suspension system in an aqueous medium, thereby preparing an agricultural composition suspension for controlling poplar psyllids.

[0053] Example 7

[0054] This embodiment provides an agricultural composition suspension for controlling Populus euphratica.

[0055] Weigh 10 parts of flupyrone, 325 parts of echinacoside, 60 parts of sodium dodecyl sulfonate, 40 parts of sodium methylene bisnaphthalene sulfonate, 60 parts of polycarboxylate, 80 parts of propylene glycol, 10 parts of carboxymethyl cellulose, 10 parts of silicone oil, and 180 parts of deionized water according to mass fractions. The above active ingredients, wetting agent, dispersant, antifreeze agent, thickener and defoamer are dispersed by high-speed shearing and sand-milled in a sand mill to form a highly dispersed and stable suspension system in an aqueous medium, thereby preparing an agricultural composition suspension for controlling poplar psyllids.

[0056] Preparation Example 8

[0057] This embodiment provides an agricultural composition suspension for controlling Populus euphratica.

[0058] Weigh 10 parts of flupyrone, 375 parts of echinacoside, 60 parts of sodium dodecyl sulfonate, 40 parts of sodium methylene bisnaphthalene sulfonate, 50 parts of polycarboxylate, 80 parts of propylene glycol, 10 parts of carboxymethyl cellulose, 10 parts of silicone oil, and 300 parts of deionized water according to mass fractions. The above active ingredients, wetting agent, dispersant, antifreeze agent, thickener and defoamer are dispersed by high-speed shearing and sand-milled in a sand mill to form a highly dispersed and stable suspension system in an aqueous medium, thereby preparing an agricultural composition suspension for controlling poplar psyllids.

[0059] Example 2

[0060] This example provides a field trial of flupyrazone and echinacoside for controlling poplar psyllids

[0061] Preparation of contrast agent: Flupyralidone with a purity of 98% purchased from Shaanxi Didu Pharmaceutical Chemical Co., Ltd. was first dissolved in dimethyl sulfoxide, and then diluted with 0.1% Tween-80 aqueous solution to prepare 17% flupyralidone as a contrast agent for standby use.

[0062] Preparation Example 1, Preparation Example 2, Preparation Example 4, Preparation Example 5, Preparation Example 7, Preparation Example 8, and the comparison agent were diluted with water at a ratio of 1:1000 to prepare different groups of liquid medicines, which were subsequently used for pest control. When the first generation of nymphs appeared in Populus euphratica in late May, the diluted liquid medicines were sprayed on the branches and leaves of Populus euphratica according to the above grouping. The number of surviving nymphs was investigated 24h, 48h, and 72h after spraying, and the nymph mortality rate was calculated according to the following formula. The statistical data was used to analyze the effect of flupyfuranone and echinacoside in the field control of Populus euphratica. After the spraying of the agent, no phytotoxic effect of the agent on Populus euphratica was observed, and the statistical results of the efficacy are shown in Table 2.

[0063] Mortality rate (%) = (number of dead insects ÷ number of live insects) × 100

[0064] Table 2 Field test results of flupyrazone and echinacoside for controlling poplar psyllid

[0065]

[0066] The results of the field test showed that the field test results were consistent with the indoor efficacy results, both of which showed that the best insecticidal effect was achieved when the mass ratio of flupyrazone to echinacoside was 1:27.5, and the insecticidal effect of the combination of flupyrazone and echinacoside was better than that of the control agent; the efficacy of the combination of flupyrazone and echinacoside on Populus euphratica in the field showed an increasing trend with the extension of time, and the insecticidal efficiency could reach up to 93.36% after spraying 72 hours.

[0067] The above-described embodiments are part of the embodiments of the present invention, but not all of them. The detailed description of the embodiments of the present invention is not intended to limit the scope of the invention claimed for protection, but rather represents selected embodiments of the present invention. All other embodiments obtained without creative work and related deductions and substitutions made by ordinary technicians in the field under the conditions of the concept of the present invention belong to the scope of protection of the present invention.

Claims

1. An agricultural composition for controlling Populus euphratica, characterized in that: The active ingredients of the agricultural composition are composed of flupyrazone and echinacoside, and the mass ratio of flupyrazone to echinacoside is 1:1-37.

5.

2. The agricultural composition for controlling Populus euphratica according to claim 1, characterized in that: The mass ratio of flupyfuranone to echinacoside is 1:10-32.

5.

3. The agricultural composition for controlling Populus euphratica according to claim 2, characterized in that: The mass ratio of flupyfuranone to echinacoside is 1:22.5-30.

4. The agricultural composition for controlling Populus euphratica according to claim 3, characterized in that: The mass ratio of flupyfuranone to echinacoside is 1:27.

5.

5. A method for controlling Populus euphratica, characterized in that: The agricultural composition for controlling poplar psyllid according to any one of claims 1 to 4 acts on poplar psyllid and / or its host.

6. Use of the agricultural composition for controlling Populus euphratica according to any one of claims 1 to 4 in the preparation of pesticides, characterized in that: The pesticide comprises the agricultural composition for controlling poplar psyllid.

7. The use according to claim 6, characterized in that: The pesticide is a suspension concentrate.

8. The use according to claim 6, characterized in that: The pesticide is an aqueous emulsion.

9. The use according to claim 6, characterized in that: The pesticide is an emulsifiable concentrate.

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