Rapid propagation and planting method for pleione bulbocodioides
Through the steps of storage of garlic orchid pods and seed germination, acid culture medium and nutrient soil are used to solve the problem of gerlic orchid seeds being difficult to germinate, and the rapid reproduction and batch cultivation of garlic orchids are achieved, reducing costs and increasing costs Growth efficiency.
Patent Information
- Application Number
- CN202411710808.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-27
- Publication Date
- 2025-02-18
AI Technical Summary
The existing breeding methods of garlic orchid seedlings cannot be cultivated in batches quickly, and garlic orchid seeds in nature are inconvenient to discharge from the pods and are extremely difficult to germinate.
Through the steps of fruit pod storage, seed germination, differentiation culture, single plant culture and seedling transplantation, acid culture and acid nutrient soil are used to promote seed germination and growth, and ultimately achieve rapid reproduction.
The rapid batch cultivation of saccharin orchids has been achieved, which has reduced the cultivation cost, shortened the growth cycle, and can produce large-scale seedlings, which has helped the protection and cultivation of saccharin orchids.
Smart Images
Figure CN119453018A_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the technical field of artificial cultivation of Cymbidium orchid, and in particular to a method for rapid propagation and planting of Cymbidium orchid. Background Art
[0002] The garlic orchid is a national second-class protected plant of the Orchidaceae family and the genus Orchidaceae, and is also a species endemic to China. The garlic orchid has a high ornamental value, with a unique flower shape, elegant and beautiful when it blooms. It can be potted or planted in patches. In addition, the garlic orchid can absorb carbon dioxide and some harmful substances in the air, improving air quality. Its leaves and stems are used as medicine to clear away heat and reduce swelling and resolve knots, and have a certain therapeutic effect on asthma, cough and joint swelling. The garlic orchid likes a cool, ventilated semi-shady environment, is relatively cold-resistant, does not hibernate in winter, and the lowest temperature for wintering is above -10℃. It is suitable for planting in loose, breathable, well-drained fern roots, sphagnum moss or humus soil.
[0003] The existing method of propagating seedlings of the garlic orchid is to obtain test tube seedlings with stable characteristics by inducing the pseudobulbs of the garlic orchid to produce protocorms. This breeding method cannot be quickly cultivated in batches. There are many seeds in the fruit pods of the garlic orchid, but in nature, its seeds are not easy to be discharged from the fruit pods, and its seeds are extremely difficult to germinate. If these seeds can be cultivated artificially, batch cultivation can be achieved, which is more conducive to the cultivation of the garlic orchid.
[0004] In view of the above technical problems, the present invention proposes a method for rapid propagation and planting of Orchidaceae using seed germination. Summary of the invention
[0005] 1. Technical issues to be solved
[0006] The present invention aims to solve the problem of obtaining test tube seedlings with stable characteristics by inducing the pseudobulbs of the garlic orchid to produce protocorms. This breeding method cannot be quickly cultivated in batches. In nature, the seeds of the garlic orchid are not easy to be discharged from the fruit pods, and the seeds are extremely difficult to germinate.
[0007] (II) Technical solution
[0008] In order to solve the above technical problems, the present invention provides a technical solution: a method for rapid propagation and planting of Orchidaceae, comprising the following steps:
[0009] (1) Fruit pod storage: After the garlic orchid has produced fruit, select the mature fruit pods, trim the dry and rotten parts at the ends of the fruit pods, and air-dry the fruit pods for 3 to 5 days to make the moisture content of the fruit pods 20% to 30%, and then seal and store for 1 to 5 months.
[0010] (2) Seed germination: Take out the sealed fruit pods, cut one end of the fruit pods, and collect the powdered seeds inside the fruit pods; pour clean water into a small culture dish, sprinkle the powdered seeds into the clean water, and the powdered seeds will float on the liquid surface. Spread the powdered seeds on the liquid surface, draw out the clean water at the bottom of the small culture dish, and pour acidic culture solution into the small culture dish. Stir evenly and soak the seeds in the culture solution for 3 to 5 days until the skin breaks and they become white balls.
[0011] (3) Differentiation culture: prepare multiple sets of large culture dishes, add clean water and acidic culture solution to the bottom of the large culture dishes, and the liquid level is 5 to 10 mm. Pour clean water into the small culture dishes again, clean the surface of the white spherical seeds with the acidic nutrient solution, and make the white spherical seeds float, so that the white spherical seeds can be salvaged through a filter spoon. The salvaged seeds are divided into multiple sets of large culture dishes, and the seeds are spread out in the large culture dishes. They are shaded and cultured for 5 to 7 days until the white spherical seeds have roots, and then light culture is carried out until green sprouts appear on the white spherical seeds.
[0012] (4) Single plant cultivation: Prepare a single plant cultivation tray, add gravel at the bottom of each cultivation groove of the single plant cultivation tray, and spread acidic nutrient soil on the upper side of the gravel. Move the seeds with green sprouts into the acidic nutrient soil, and spread 2 to 5 mm of acidic nutrient soil on the upper side of the seeds to cover the seeds.
[0013] (5) Transplanting of seedlings: When the plants grow to 10-20 mm, remove the seedlings from the cultivation tray and plant them outdoors.
[0014] (6) Artificial breeding: After the garlic orchids grown outdoors bloom, artificial pollination is carried out.
[0015] As an improvement, the pH value of the acidic culture solution and the acidic nutrient soil is 5-6.
[0016] As an improvement, the structure of the small culture dish in step (1) includes a frame, the bottom of the frame has multiple groups of supporting legs, the bottom of the frame is provided with a drainage pipe, and the drainage pipe is provided with a water control valve.
[0017] As an improvement, in step (1), the initial height of the clean water level in the small culture dish is 10 to 30 mm, the liquid level is 2 to 5 mm after a portion of the clean water at the bottom of the small culture dish is discharged through a drain pipe, and the liquid level is 5 to 10 mm after acidic culture medium is injected into the clean water.
[0018] As an improvement, the structure of the single plant cultivation tray in step (4) includes a frame, the bottom of the frame is a water filter plate, a mesh plate is placed inside the frame, and the meshes in the mesh plate are the cultivation troughs proposed in step (4).
[0019] As an improvement, when the seeds with green sprouts in step (4) are moved to the acidic nutrient soil inside the mesh plate, the roots are facing downward and the green sprouts are facing upward, and the lower part of the seeds is lightly pressed into the acidic nutrient soil to ensure stable growth of the seeds.
[0020] As an improvement, the acidic nutrient soil in step (4) is sprayed with water to keep the acidic nutrient soil moist.
[0021] After adopting the above method, the present invention has the following advantages: utilizing the seed germination propagation method, rapid batch cultivation can be carried out, the cultivation cost is low, the growth cycle is short, and a large number of seedlings can be produced, which is beneficial to the cultivation of the single garlic orchid, can increase the reserves of the single garlic orchid, and is beneficial to the protection of the endangered single garlic orchid. BRIEF DESCRIPTION OF THE DRAWINGS
[0022] Figure 1 It is a structural schematic diagram of a small culture dish in a method for rapid propagation and planting of Orchidaceae of the present invention.
[0023] Figure 2 It is a schematic diagram of the upper structure of a single-plant cultivation tray in a method for rapid propagation and planting of Solanum sibiricum of the present invention.
[0024] Figure 3 It is a schematic diagram of the lower structure of a single-plant cultivation tray in a method for rapid propagation and planting of a single garlic orchid of the present invention. DETAILED DESCRIPTION
[0025] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments; based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.
[0026] Example 1
[0027] A method for rapid propagation and planting of Orchidaceae, comprising the following steps:
[0028] (1) Fruit pod storage: After the garlic orchid has produced fruit, select mature fruit pods, trim the dry and rotten parts at the ends of the fruit pods, and air-dry the fruit pods for 4 days to make the moisture content of the fruit pods 28%. Seal and store for 3 months. During these 3 months, the fruit pods will not get damp, so that the seeds inside the fruit pods remain dry.
[0029] (2) Seed germination: Take out the sealed fruit pods, cut one end of the fruit pods, knock on the outside of the fruit pods, shake out the powdered seeds inside the fruit pods, and collect them; pour clean water into a small culture dish, sprinkle the powdered seeds into the clean water, the powdered seeds will float on the liquid surface, spread the powdered seeds on the liquid surface, as shown in the attached Figure 1As shown, the structure of the small culture dish includes a frame, the bottom of the frame has multiple groups of supporting legs, the bottom of the frame is provided with a drain pipe, the drain pipe is provided with a water control valve, the water control valve is opened, and the clean water at the bottom of the small culture dish is pumped out through the drain pipe, and the powdered seeds on the liquid surface will not flow out of the drain pipe. The initial height of the clean water level in the small culture dish is 15 mm, and the liquid level is 3 mm after a part of the clean water at the bottom of the small culture dish is discharged through the drain pipe. Acidic culture solution is injected into the small culture dish, and the liquid level after the acidic culture solution is injected into the clean water is 7 mm. The pH value of the acidic culture solution is 5.8, and the solution is stirred evenly. The seeds are soaked in the culture solution for 4 days until the skin is broken and the seeds are white spherical.
[0030] (3) Differentiation culture: prepare multiple sets of large culture dishes, add clean water and acidic culture solution to the bottom of the large culture dishes, and the liquid level is 6 mm. Pour clean water into the small culture dishes again. The clean water can clean the acidic nutrient solution on the surface of the white spherical seeds and make the white spherical seeds float, making it easy to salvage the white spherical seeds with a filter spoon. The salvaged seeds are divided into multiple sets of large culture dishes. The seeds are spread out in the large culture dishes and cultured in a shaded environment for 6 days. The white spherical seeds will have roots. Then, they are cultured in light, and green sprouts will appear on the white spherical seeds.
[0031] (4) Single plant cultivation: Prepare a single plant cultivation tray, as shown in the attached Figure 2 With attached Figure 3 As shown, the structure of the single-plant cultivation tray includes a frame, the bottom of the frame is a water filter plate, a mesh plate is placed inside the frame, gravel is added to the bottom of each group of cultivation grooves of the single-plant cultivation tray, and acidic nutrient soil is spread on the upper side of the gravel to make the acidic nutrient soil moist and breathable, which is conducive to seed growth, and seeds with green sprouts are moved to the acidic nutrient soil. The pH value of the acidic culture solution is 5.8. When the seeds with green sprouts are moved to the acidic nutrient soil inside the mesh tray, the roots of the seeds are facing downward and the green sprouts are facing upward. The lower part of the seeds is slightly pressed into the acidic nutrient soil to ensure the stability of seed growth, and 3mm of acidic nutrient soil is spread on the upper side of the seeds to cover the seeds. The acidic nutrient soil is sprayed with water to keep the acidic nutrient soil moist.
[0032] (5) Transplanting of seedlings: When the plants grow to 15 mm, remove the seedlings from the cultivation tray and plant them outdoors.
[0033] (6) Artificial breeding: After the garlic orchids grown outdoors bloom, artificial pollination is carried out.
[0034] Example 2
[0035] A method for rapid propagation and planting of Orchidaceae, comprising the following steps:
[0036] (1) Fruit pod storage: After the garlic orchid has produced fruit, select mature fruit pods, trim the dry and rotten parts at the ends of the fruit pods, and air-dry the fruit pods for 4 days to make the moisture content of the fruit pods 22%. Store them in sealed containers for 4.5 months. During these 4.5 months, the fruit pods will not get damp, so that the seeds inside the fruit pods remain dry.
[0037] (2) Seed germination: Take out the sealed fruit pods, cut one end of the fruit pods, knock on the outside of the fruit pods, shake out the powdered seeds inside the fruit pods, and collect them; pour clean water into a small culture dish, sprinkle the powdered seeds into the clean water, the powdered seeds will float on the liquid surface, spread the powdered seeds on the liquid surface, as shown in the attached Figure 1 As shown, the structure of the small culture dish includes a frame, the bottom of the frame has multiple groups of supporting legs, the bottom of the frame is provided with a drain pipe, the drain pipe is provided with a water control valve, the water control valve is opened, and the clean water at the bottom of the small culture dish is pumped out through the drain pipe, and the powdered seeds on the liquid surface will not flow out of the drain pipe. The initial height of the clean water level in the small culture dish is 25 mm, and the liquid level is 4 mm after a part of the clean water at the bottom of the small culture dish is discharged through the drain pipe. Acidic culture solution is injected into the small culture dish, and the liquid level after the acidic culture solution is injected into the clean water is 8 mm. The pH value of the acidic culture solution is 5.2, and the solution is stirred evenly. The seeds are soaked in the culture solution for 5 days until the skin is broken and the seeds are white spherical.
[0038] (3) Differentiation culture: prepare multiple sets of large culture dishes, add clean water and acidic culture solution to the bottom of the large culture dishes, and the liquid level is 6 mm. Pour clean water into the small culture dishes again. The clean water can clean the acidic nutrient solution on the surface of the white spherical seeds and make the white spherical seeds float, making it easy to salvage the white spherical seeds with a filter spoon. The salvaged seeds are divided into multiple sets of large culture dishes, and the seeds are spread out in the large culture dishes. They are shaded and cultured for 5 days. The white spherical seeds will have roots. Then they are cultured in light, and green sprouts will appear on the white spherical seeds.
[0039] (4) Single plant cultivation: Prepare a single plant cultivation tray, as shown in the attached Figure 2 With attached Figure 3 As shown, the structure of the single-plant cultivation tray includes a frame, the bottom of the frame is a water filter plate, a mesh plate is placed inside the frame, gravel is added to the bottom of each group of cultivation grooves of the single-plant cultivation tray, and acidic nutrient soil is spread on the upper side of the gravel to make the acidic nutrient soil moist and breathable, which is conducive to seed growth, and seeds with green sprouts are moved to the acidic nutrient soil. The pH value of the acidic culture solution is 5. When the seeds with green sprouts are moved to the acidic nutrient soil inside the mesh tray, the roots of the seeds are facing downward and the green sprouts are facing upward. The lower part of the seeds is slightly pressed into the acidic nutrient soil to ensure the stability of seed growth, and 4mm of acidic nutrient soil is spread on the upper side of the seeds to cover the seeds. The acidic nutrient soil is sprayed with water to keep the acidic nutrient soil moist.
[0040] (5) Transplanting of seedlings: When the plants grow to 18 mm, remove the seedlings from the cultivation troughs in the cultivation tray and plant them outdoors.
[0041] (6) Artificial breeding: After the garlic orchids grown outdoors bloom, artificial pollination is carried out.
[0042] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.
[0043] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.
[0044] The present invention and its embodiments are described above, and such description is not restrictive. The drawings show only one embodiment of the present invention, and the actual structure is not limited thereto. In short, if ordinary technicians in the field are inspired by it, without departing from the purpose of the invention, they can design a structure and embodiment similar to the technical solution without creativity, which should belong to the protection scope of the present invention.
Claims
1. A method for rapid propagation and planting of Orchidaceae, characterized in that: The following steps are involved: (1) Fruit pod storage: After the garlic orchid has produced fruit, select the mature fruit pods, trim the dry and rotten parts at the ends of the fruit pods, dry the fruit pods for 3 to 5 days to make the moisture content of the fruit pods 20% to 30%, and seal and store for 1 to 5 months; (2) Seed germination: Take out the sealed fruit pods, cut open one end of the fruit pods, take out the powdered seeds inside the fruit pods and collect them; pour clean water into a small culture dish, sprinkle the powdered seeds into the clean water, the powdered seeds will float on the liquid surface, spread the powdered seeds on the liquid surface, draw out the clean water at the bottom of the small culture dish, pour acidic culture solution into the small culture dish, stir evenly, and soak the seeds in the culture solution for 3 to 5 days until the skin breaks and they become white balls; (3) Differentiation culture: prepare multiple sets of large culture dishes, add clean water and acidic culture solution to the bottom of the large culture dishes, and the liquid level is 5 to 10 mm. Pour clean water into the small culture dishes again, wash the surface of the white spherical seeds with the acidic nutrient solution, and make the white spherical seeds float, so that the white spherical seeds can be salvaged with a filter spoon. The salvaged seeds are divided into multiple sets of large culture dishes, and the seeds are spread out in the large culture dishes. They are shaded and cultured for 5 to 7 days until the white spherical seeds have roots, and then light culture is carried out until green sprouts appear on the white spherical seeds. (4) Single plant cultivation: Prepare a single plant cultivation tray, add gravel at the bottom of each cultivation groove of the single plant cultivation tray, and spread acidic nutrient soil on the upper side of the gravel. Move the seeds with green sprouts into the acidic nutrient soil, and spread 2-5 mm of acidic nutrient soil on the upper side of the seeds to cover the seeds; (5) Transplanting seedlings: When the plants grow to 10-20 mm, the seedlings are removed from the cultivation trays and planted outdoors; (6) Artificial breeding: After the garlic orchids grown outdoors bloom, artificial pollination is carried out.
2. A method for rapid propagation and planting of Orchidaceae according to claim 1, characterized in that: The pH values of the acidic culture solution and the acidic nutrient soil are 5-6.
3. A method for rapid propagation and planting of Orchidaceae according to claim 1, characterized in that: The structure of the small culture dish described in step (1) comprises a frame, a plurality of support legs are provided at the bottom of the frame, a drainage pipe is provided at the bottom of the frame, and a water control valve is provided on the drainage pipe.
4. A method for rapid propagation and planting of Orchidaceae according to claim 3, characterized in that: Step (1) The initial height of the clean water level in the small culture dish is 10-30 mm, the liquid level is 2-5 mm after a portion of the clean water at the bottom of the small culture dish is discharged through the drain pipe, and the liquid level is 5-10 mm after the acidic culture medium is injected into the clean water.
5. The method for rapid propagation and planting of Orchidaceae according to claim 1, characterized in that: The structure of the single plant cultivation tray in step (4) includes a frame, a water filter plate at the bottom of the frame, a mesh plate placed inside the frame, and the meshes in the mesh plate are the cultivation troughs proposed in step (4).
6. A method for rapid propagation and planting of Orchidaceae according to claim 5, characterized in that: In step (4), when the seeds with green sprouts are moved to the acidic nutrient soil inside the mesh plate, the roots are facing downward and the green sprouts are facing upward, and the lower part of the seeds is slightly pressed into the acidic nutrient soil to ensure stable growth of the seeds.
7. The method for rapid propagation and planting of Orchidaceae according to claim 1, characterized in that: The acidic nutrient soil in step (4) is sprayed with water to keep the acidic nutrient soil moist.