Ani Thai stock solution traditional Chinese medicine composition as well as preparation method and application thereof

Abstract

The invention provides an anal-Thai stock solution traditional Chinese medicine composition and a preparation method and application thereof, and belongs to the technical field of medicine preparation.The preparation method comprises the steps that 5-15 parts by weight of cortex phellodendri, 3-12 parts by weight of radix sophorae flavescentis, 3-10 parts by weight of sophora flower bud, 3-10 parts by weight of honeysuckle, 1-5 parts by weight of sanguisorba officinalis, 3-10 parts by weight of lalang grass rhizome, 3-10 parts by weight of fructus aurantii, 1-5 parts by weight of pseudo-ginseng and 1-5 parts by weight of corydalis tuber are taken; performing soaking, enzymolysis and ultrasonic extraction to prepare the anal-Thai stock solution. The anotei stock solution is used for preparing products in different dosage forms, such as sanitary wet tissues, wet toilet paper, antibacterial spray, antibacterial lotion or antibacterial gel. The anorectal stoste prepared by the invention is exact in curative effect, is prepared by taking genuine traditional Chinese medicinal materials as raw materials according to a reasonable ratio and a modern biological extraction technology, and has good effects of inhibiting bacteria, relieving itching, diminishing swelling and relieving pain on patients suffering from haemorrhoids.

Description

Technical Field

[0001] The invention relates to the preparation of traditional Chinese medicine, and in particular to an Antai stock solution traditional Chinese medicine composition and a preparation method and application thereof. Background Art

[0002] Hemorrhoids are a common disease located in the anus. They can occur at any age, but the incidence rate gradually increases with age, and is more common in people aged 20 to 60. Hemorrhoids are divided into internal hemorrhoids, external hemorrhoids, and mixed hemorrhoids according to the different locations of occurrence. The clinical manifestations of hemorrhoids are anal itching, swelling and pain, bleeding, and even ulceration. According to relevant census data, the incidence rate of anorectal diseases is 59.1%, and hemorrhoids account for 87.25% of all anorectal diseases, among which internal hemorrhoids account for 59.86%, external hemorrhoids account for 16.1%, and mixed hemorrhoids account for 24.13%. Therefore, the overall incidence rate of hemorrhoids is about 50%, so there is a saying that "nine out of ten people have hemorrhoids". The pain caused by hemorrhoids to patients is unbearable, and severe patients are restless, affecting normal work and life. Therefore, it is particularly important to treat hemorrhoids in a timely manner.

[0003] Western medicine mainly treats hemorrhoids with surgical therapy, ultra-low temperature and ultra-high temperature therapy, sleeve and ligation therapy, and injection therapy. These therapies are expensive, have a long recovery period, and are prone to complications after the disease. Traditional Chinese medicine emphasizes the principle of syndrome differentiation and treatment in the treatment of hemorrhoids. The treatment of hemorrhoids can be divided into external use and internal treatment. In ancient times, internal treatment of hemorrhoids mostly advocated clearing away heat and detoxification, cooling blood and removing blood stasis, and treating according to different syndrome types; the external treatment methods of traditional Chinese medicine for hemorrhoids mainly include fumigation therapy, compress therapy, and suppository therapy. Traditional Chinese medicine has the advantages of significant efficacy, treating both the symptoms and the root causes, and few drug side effects in the treatment of hemorrhoids, which has led to its rapid application and promotion in the field of hemorrhoidal diseases.

[0004] However, 90% of Chinese medicines are herbal medicines with complex chemical compositions. The extraction of traditional Chinese medicine generally adopts decoction, water distillation and solvent extraction, and the extraction efficiency of its effective components is low, and the specificity of the components is not strong. The main reason is that the effective components of most plants are wrapped in cell walls, and it is difficult to extract completely with water as solvent. In addition, the existing Chinese medicine prescription compatibility is unreasonable, resulting in the current therapeutic effect of hemorrhoidal diseases not being significant, and the cure rate is low. Therefore, studying a new Chinese medicine for the treatment of hemorrhoids and a new Chinese medicine extraction method is an urgent demand for Chinese medicine refining, which is most important for the development of Chinese medicine for the treatment of hemorrhoids.

[0005] At present, there are also related technologies about hemorrhoids Chinese medicine disclosed, such as: The Chinese invention patent application with the publication number CN 116832122 A discloses a Chinese medicine composition, preparation, preparation method, use method and application thereof, and specifically discloses that the Chinese medicine composition for hemorrhoids includes: rhubarb, gentian, white flower vine, three-leaf bitter leaf, honeysuckle, Dictamni, calendula, borneol, mint, ice silkworm, coptis root, pure bezoar, chlorhexidine acetate, red ochre and gastrodia seed. However, the Chinese medicine composition used in the invention has many components and unreasonable compatibility. Among them, rhubarb, gentian and Dictamni can be absorbed through the skin for a long time, causing liver damage; chlorhexidine acetate is a chemical antibacterial component, which shows that the compatibility of the prescription is unreasonable.

[0006] The Chinese invention patent application with publication number CN 116920037 A discloses a Chinese medicine composition for treating hemorrhoids, and specifically discloses that the Chinese medicine composition for treating hemorrhoids includes: 15% to 30% of Tripterygium wilfordii, 5% to 13% of Cimicifuga heracleifolia, 3% to 15% of Fig leaves, 3% to 10% of Portulaca oleracea, 3% to 10% of Sophora japonica, 3% to 10% of Sanguisorba officinalis, 1% to 5% of Honeysuckle, 2% to 8% of Artemisia capillaris, 1% to 6% of Smilax glabra, 1% to 8% of Verbena officinalis, 1% to 7% of Sophora japonica, 1% to 9% of Polygonum cuspidatum, and 1% to 9% of Indigo naturalis, and is prepared by alcohol extraction. Although alcohol extraction can increase the dissolution of effective ingredients, there is a problem of poor control of the extraction time. Too short an extraction time will lead to incomplete extraction, and too long an extraction time will lead to a reduction in the efficacy of the ingredients. And the overall formula is complex and the compatibility is unreasonable.

[0007] The Chinese invention patent application with publication number CN 117482141 A discloses a Chinese medicine composition for treating hemorrhoids and its preparation method, and specifically discloses that the Chinese medicine composition is prepared from six Chinese medicines, namely, Radix Sanguisorbae, Fructus Sophorae, Semen Cassiae, Concha Ostreae, Ginseng and Radix Scutellariae, through a traditional decoction process. Although the Chinese medicine composition of the invention is concise and targeted, it is difficult to completely extract the effective components by the traditional preparation process, and the effect will be greatly reduced. And the overall efficacy needs to be improved.

[0008] In view of the above problems, the present invention utilizes traditional Chinese medicine theory combined with modern biotechnology - enzymatic hydrolysis technology and ultrasonic technology to develop a rapid, environmentally friendly, efficient and economical extraction technology, laying a foundation for the development of Chinese medicine refining technology. Summary of the invention

[0009] In view of the above problems, the present invention provides a Chinese medicine composition of An Tai stock solution and a preparation method and application thereof.

[0010] To achieve the above purpose, the technical solution adopted by the present invention is: A Chinese medicine composition of Antai stock solution, wherein the prescription of the Chinese medicine composition of Antai stock solution is: 5-15 parts of Phellodendron amurense, 3-12 parts of Sophora flavescens, 3-10 parts of Sophora japonica seeds, 3-10 parts of Honeysuckle, 1-5 parts of Sanguisorba officinalis, 3-10 parts of Imperata cylindrica, 3-10 parts of Citrus aurantium, 1-5 parts of Panax notoginseng and 1-5 parts of Corydalis yanhusuo; Preferably, 10 parts of Phellodendron chinense, 8 parts of Sophora flavescens, 6 parts of Sophora japonica seeds, 6 parts of Honeysuckle, 3 parts of Sanguisorba officinalis, 6 parts of Imperata cylindrica, 6 parts of Fructus Aurantii Immaturus, 3 parts of Panax notoginseng and 3 parts of Corydalis yanhusuo.

[0011] A method for preparing an An Tai stock solution, the method comprising taking the above-mentioned An Tai stock solution Chinese medicine composition, soaking, enzymolysis, and ultrasonic extraction to prepare the An Tai stock solution; Alternatively, the preparation method is to take the above-mentioned An Tai stock solution Chinese medicine composition and use water extraction method to prepare the An Tai stock solution.

[0012] Furthermore, the preparation method comprises the following steps: S1. Take Phellodendron amurense, Sophora flavescens, Sophora japonica seeds, Lonicera japonica, Sanguisorba officinalis, Imperata cylindrica, Fructus aurantii, Panax notoginseng and Corydalis yanhusuo, crush them, sieve them, soak them in water and obtain a soaked medicinal material system; Take cellulase, pectinase and hemicellulase to prepare a complex enzyme solution; S2, adding the complex enzyme solution to the soaked medicinal material system, performing enzymolysis, inactivating the enzyme, filtering, and obtaining a filtrate; S3, the filtrate is subjected to ultrasonic extraction, and the precipitate is separated by centrifugation to obtain a centrifuge; S4. The centrifuged liquid is sterilized to obtain the An Tai stock solution.

[0013] Further, the weight ratio of cellulase, pectinase and hemicellulase is 2-4:2-4:1-2, preferably 2:2:1; The enzyme concentration in the complex enzyme solution is 0.1-1.0%, preferably 0.3-0.8%; The pH value of the complex enzyme solution is 3.0-6.0, preferably 4.5-6.0; In the process of preparing the complex enzyme solution, the solvent used is sodium citrate-citric acid buffer, phosphate buffer, acetate buffer or disodium hydrogen phosphate-citric acid buffer.

[0014] Furthermore, the temperature of enzymatic hydrolysis is 30-66°C and the time is 1-4h.

[0015] Furthermore, the temperature of ultrasonic extraction is 40-50°C, the frequency is 35-45kHz, and the time is 30-90min, preferably the temperature is 45°C, the frequency is 40kHz, and the time is 60-75min.

[0016] Furthermore, the soaking is performed by adding 8 to 13 times the weight of the prescribed amount of water for 2 to 3 hours, preferably adding 8 to 10 times the weight of the prescribed amount of water.

[0017] Furthermore, the An Tai stock solution is 5 to 10 times by weight of the prescribed amount.

[0018] Furthermore, the sieve hole diameter is 12-24 mesh.

[0019] An application of An Tai stock solution, which is an An Tai stock solution made from the above-mentioned An Tai stock solution Chinese medicine composition, and the An Tai stock solution is used to prepare products of different dosage forms such as sanitary wet wipes, wet toilet paper, antibacterial spray, antibacterial lotion or antibacterial gel.

[0020] The beneficial effects of the Antai stock solution Chinese medicine composition and its preparation method and application of the present invention are: The prescription of the invention is reasonable. The prescription adopted is based on the traditional Chinese medicine theory and the principle of Chinese medicine dialectical treatment. Aiming at the causes of wind, heat, dampness, dryness and the like causing the disease, the ratio of monarch, minister, assistant and envoy is used. Phellodendron chinense is used as the monarch medicine to exert the effects of clearing heat and drying dampness, purging fire and detoxifying, and stopping bleeding; Sophora flavescens, Sophora japonica seed, honeysuckle, Sanguisorba officinalis and Imperata cylindrica are used as the assistant medicines, among which Sophora flavescens clears heat and dries dampness to stop itching, Sophora japonica seed cools blood and stops bleeding, Honeysuckle clears heat and detoxifies, Sanguisorba officinalis astringes and stops bleeding, Imperata cylindrica cools blood, and the monarch medicine clears heat and dries dampness to stop itching is strengthened; Fructus aurantii and Panax notoginseng are used as the assistant medicines, and have the effects of promoting blood circulation and regulating qi; Corydalis yanhusuo is used as the envoy medicine, and can promote blood circulation and stop pain; the combination of the medicines can achieve the therapeutic effect of treating both the symptoms and the root causes of the hemorrhoids, the itching, swelling and bleeding, and the prescription is concise and has no toxic side effects; The present invention adopts advanced extraction technology, and greatly increases the dissolution of effective components of traditional Chinese medicine by combining modern biotechnology-enzymatic hydrolysis technology and ultrasonic extraction technology; the preparation method has mild reaction, does not destroy the original three-dimensional structure and biological activity of the active components, has short extraction time, high extraction rate, high reaction specification, and simple operation. It is a fast, environmentally friendly, efficient and economical extraction technology, which lays a foundation for the development of traditional Chinese medicine refining technology; The An Tai stock solution prepared by the invention has definite curative effect. Its components are prepared from authentic Chinese medicinal materials through reasonable proportioning and modern biological extraction technology. It has good antibacterial, antipruritic, anti-swelling and analgesic effects on patients with hemorrhoids, and can be used for the treatment of internal hemorrhoids, external hemorrhoids and mixed hemorrhoids. It has good curative effect, quick effect, safety and no side effects. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a spectrum of the content of the active ingredient contained in the Antai stock solution DG1 prepared in Example 1 of the present invention; Figure 2 is a spectrum of the active ingredient content contained in the Antai stock solution G1 prepared in Example 1 of the present invention; Figure 3This is a diagram of the antibacterial effect in the antibacterial test of Example 4 of the present invention; Figure 4 It is a rendering of the effect of the anorectal model repair experiment of Experimental Group 2 in the swelling and pain relief test of Example 4 of the present invention. DETAILED DESCRIPTION

[0022] The technical solutions in the embodiments of the present invention are described clearly and completely below. In the following description, many specific details are set forth to facilitate a full understanding of the present invention, but the present invention can also be implemented in other ways different from those described herein, and those skilled in the art can make similar generalizations without violating the connotation of the present invention, so the present invention is not limited by the specific embodiments disclosed below.

[0023] Example 1 A method for preparing Antai stock solution 1. Preparation of Antai stock solution This embodiment is a method for preparing a kind of Antai stock solution, adopting modern biotechnology-enzymatic hydrolysis technology and ultrasonic technology, specifically comprising the following steps: S1, take nine medicinal materials, including 10g of Phellodendron amurense, 8g of Sophora flavescens, 6g of Sophora japonica seeds, 6g of Lonicera japonica, 3g of Sanguisorba officinalis, 6g of Imperata cylindrica, 6g of Fructus Aurantii Immaturus, 3g of Panax notoginseng and 3g of Corydalis yanhusuo, grind them according to the proportion, pass through a 24-mesh sieve, add 8 times the weight of the prescription amount of water, and soak them for 2 hours to obtain a soaked medicinal material system; Take cellulase, pectinase and hemicellulase in a weight ratio of 2:2:1, add them to acetic acid-sodium acetate buffer with a pH value of 4.5 and mix well to prepare a composite enzyme solution with an enzyme concentration of 0.5% and a pH value of 4.5.

[0024] S2. Add the complex enzyme solution to the soaked medicinal material system, fully enzymolyze in a constant temperature water bath at 45°C for 2 hours, then heat to 100°C, boil for 10 minutes to inactivate the enzyme, take out, cool, and filter to obtain a filtrate.

[0025] S3. The filtrate was transferred to an ultrasonic instrument and ultrasonically extracted for 60 min at a temperature of 45°C and a frequency of 40 kHz. After the reaction was completed, the precipitate was separated by centrifugation to obtain a centrifuge.

[0026] S4. Take the centrifuged liquid and sterilize it at high temperature at 130℃ for 30s to obtain a 1:5 extract, which is the Antai stock solution, marked as G1.

[0027] Among them, the 1:5 in the 1:5 times extract represents the weight ratio between the total weight of the nine medicinal materials (that is, the prescription amount) and the extract, that is, the An Tai original solution is 5 times the weight of the prescription amount.

[0028] 2. Preparation method of control Antai stock solution Furthermore, the extract prepared by the traditional preparation process water extraction method was used as the control Antai stock solution, and the specific steps were as follows: Take nine medicinal materials, including 10g of Phellodendron chinense, 8g of Sophora flavescens, 6g of Sophora japonica seed, 6g of Lonicera japonica, 3g of Sanguisorba officinalis, 6g of Imperata cylindrica, 6g of Citrus aurantium, 3g of Panax notoginseng and 3g of Corydalis yanhusuo (the total weight of the nine medicinal materials is the prescription amount), grind them according to the proportion, pass through a 24-mesh sieve, add 8 times the prescription amount of water, soak them for 2 hours, then decoct and extract twice, each time for 1 hour, combine the decoctions, filter, and concentrate the filtrate to 5 times the weight of the prescription amount to obtain a 1:5 control extract, which is the control An Tai stock solution, marked as DG1.

[0029] Analysis The content and extraction rate of the active ingredients in Antai stock solution G1 and the control Antai stock solution DG1 were compared. The results are shown in Table 1 and Figure 1~Figure 2 As shown: Table 1 Comparison of the content and extraction rate of each component under different process conditions

[0030] As can be seen from Table 1, the content of effective ingredients contained in the An Tai stock solution G1 extracted by modern biotechnology-enzymatic hydrolysis technology and ultrasonic technology is significantly higher than that in the An Tai stock solution DG1 prepared by water extraction method, and the extraction rate is significantly increased, which is beneficial to improving efficacy and reducing costs.

[0031] Example 2 A method for preparing an Antai stock solution This embodiment is a method for preparing a GANTAI stock solution, which specifically comprises the following steps: S1, take nine medicinal materials, including 10g of Phellodendron amurense, 8g of Sophora flavescens, 6g of Sophora japonica seeds, 6g of Lonicera japonica, 3g of Sanguisorba officinalis, 6g of Imperata cylindrica, 6g of Fructus Aurantii Immaturus, 3g of Panax notoginseng and 3g of Corydalis yanhusuo, grind them according to the proportion, pass through a 12-mesh sieve, add 10 times the weight of the prescribed amount of water, and soak them for 3 hours to obtain a soaked medicinal material system; Take cellulase, pectinase and hemicellulase in a weight ratio of 3:3:1, add them to acetic acid-sodium acetate buffer with a pH value of 5.0 and mix well to prepare a composite enzyme solution with an enzyme concentration of 0.5% and a pH value of 5.0.

[0032] S2. Add the complex enzyme solution to the soaked medicinal material system, fully enzymolyze in a constant temperature water bath at 50°C for 3 hours, then heat to 100°C, boil for 10 minutes to inactivate the enzyme, take out, cool, and filter to obtain a filtrate.

[0033] S3. The filtrate was transferred to an ultrasonic instrument and ultrasonically extracted for 75 min at a temperature of 45°C and a frequency of 40 kHz. After the reaction was completed, the precipitate was separated by centrifugation to obtain a centrifuge.

[0034] S4. Take the centrifuged liquid and sterilize it at high temperature at 130℃ for 30s to obtain a 1:7.2 extract, which is the Antai stock solution and is marked as G2.

[0035] Among them, the 1:7.2 in 1:7.2 times extract represents the weight ratio between the total weight of the nine medicinal materials and the extract, that is, the An Tai original solution is 7.2 times the weight of the prescription amount.

[0036] S5. Compare the effective ingredient content and extraction rate of An Tai stock solution G2 and the control An Tai stock solution DG1 prepared in Example 1. The results are shown in Table 2: Table 2 Comparison of the content and extraction rate of each component under different process conditions

[0037] As can be seen from Table 2, the content of effective ingredients contained in the An Tai stock solution G2 extracted by modern biotechnology-enzymatic hydrolysis technology and ultrasonic technology is significantly higher than that in the An Tai stock solution DG1 prepared by water extraction method, and the extraction rate is significantly increased, which is beneficial to improve the efficacy and reduce costs.

[0038] Example 3 A method for preparing Antai stock solution This embodiment is a method for preparing a GANTAI stock solution, which specifically comprises the following steps: S1, take nine medicinal materials, including 10g of Phellodendron amurense, 8g of Sophora flavescens, 6g of Sophora japonica seeds, 6g of Lonicera japonica, 3g of Sanguisorba officinalis, 6g of Imperata cylindrica, 6g of Citrus aurantium, 3g of Panax notoginseng and 3g of Corydalis yanhusuo, grind them according to the proportion, pass through a 24-mesh sieve, add 13 times the weight of the prescribed amount of water, and soak them for 2 hours to obtain a soaked medicinal material system; Cellulase, pectinase and hemicellulase were taken in a weight ratio of 2:2:1, added to an acetic acid-sodium acetate buffer solution with a pH value of 5.5 and mixed to prepare a composite enzyme solution with an enzyme concentration of 1.0% and a pH value of 5.5.

[0039] S2. Add the complex enzyme solution to the soaked medicinal material system, fully enzymolyze in a constant temperature water bath at 55°C for 2 hours, then heat to 100°C, boil for 10 minutes to inactivate the enzyme, take out, cool, and filter to obtain a filtrate.

[0040] S3. The filtrate was transferred to an ultrasonic instrument and ultrasonically extracted for 90 min at a temperature of 45°C and a frequency of 40 kHz. After the reaction was completed, the precipitate was separated by centrifugation to obtain a centrifuge.

[0041] S4. Take the centrifuged liquid and sterilize it at high temperature at 130℃ for 30s to obtain a 1:9.5 extract, which is the Antai stock solution and is marked as G3.

[0042] Among them, the 1:9.5 in the 1:9.5 times extract represents the weight ratio between the total weight of the nine medicinal materials and the extract, that is, the An Tai original solution is 9.5 times the weight of the prescription amount.

[0043] S5. Compare the effective ingredient content and extraction rate of An Tai stock solution G3 and the control An Tai stock solution DG1 prepared in Example 1. The results are shown in Table 3: Table 3 Comparison of the content and extraction rate of each component under different process conditions

[0044] As can be seen from Table 3, the content of effective ingredients contained in the An Tai stock solution G3 extracted by modern biotechnology-enzymatic hydrolysis technology and ultrasonic technology is significantly higher than that in the An Tai stock solution DG1 prepared by water extraction method, and the extraction rate is significantly increased, which is beneficial to improve the efficacy and reduce costs.

[0045] Example 4 Application of Antai stock solution The Antai stock solution G1 prepared in Example 1 and the control Antai stock solution DG1 were used to prepare hemorrhoid gel and hemorrhoid spray, respectively, as follows: 1) Preparation of hemorrhoid gel According to weight percentage, take 1.8% of medium-viscosity hydroxyethyl cellulose (the viscosity is generally 3000~10000cps), 50% of the corresponding AnTai stock solution (i.e. AnTai stock solution G1 or control AnTai stock solution DG1), 5% of glycerin, 2% of propylene glycol, and 41.2% of purified water to prepare the corresponding hemorrhoid gel (i.e. AnTai stock solution G1 is prepared into hemorrhoid gel GJ1, and AnTai stock solution DG1 is prepared into hemorrhoid gel DGJ1), and test its antibacterial, antipruritic and repair effects.

[0046] 2) Preparation of hemorrhoid spray According to weight percentage, take 0.5% of low-viscosity hydroxyethyl cellulose (viscosity is generally 10~500cps), 50% of the corresponding AnTai stock solution (i.e. AnTai stock solution G1 or control AnTai stock solution DG1), 5% of glycerin, 2% of propylene glycol, and 42.5% of purified water, and prepare them into hemorrhoid spray (i.e. AnTai stock solution G1 is configured into hemorrhoid spray GW1, and AnTai stock solution DG1 is configured into hemorrhoid spray DGW1) to test its antibacterial, antipruritic and repair effects.

[0047] 3) Antibacterial test 31) Experimental methods Test basis: "Disinfection Technical Specifications" (2002 edition); Test conditions: test environment temperature is 20.1~22.5℃, relative humidity is 49~53%RH.

[0048] 32) Experimental results The two hemorrhoid sprays were subjected to antibacterial experiments. Hemorrhoid spray DGW1 was used as experimental group 1, hemorrhoid spray GW1 was used as experimental group 2, and sodium chloride buffer was used as a positive control. Microbial inhibition experiments were performed on Staphylococcus aureus, Escherichia coli, and Candida albicans. The results are shown in Tables 4 and Figure 3 .

[0049] Table 4 Antibacterial test results list

[0050] Note: The evaluation standard is based on the 2002 edition of the Technical Specifications for Disinfection; if the antibacterial rate is greater than 50-90%, the product has an antibacterial effect; if the antibacterial rate is greater than 90%, it has a strong antibacterial effect.

[0051] From Table 4 and Figure 3 It can be seen that the hemorrhoid spray GW1 and hemorrhoid spray DGW1 prepared by the present invention both have good antibacterial effects, among which the antibacterial effect of hemorrhoid spray GW1 is significantly better than that of hemorrhoid spray DGW1.

[0052] 4) Anti-itch test 41) Experimental Materials Experimental animals: SPF grade male rats.

[0053] Reagents: acetone, ether, distilled water, chloral hydrate, depilatory cream, etc.

[0054] Instruments: HD camera, etc.

[0055] 42) Animal modeling and experimental methods Eighteen SPF mice weighing 20-25 g were randomly divided into three groups, with 6 mice in each group, namely experimental group 1, experimental group 2 and control group. After 10% chloral hydrate anesthesia was used in each group, the hair on the back of the neck was shaved (from the line connecting the two ears to the second thoracic vertebrae, with an area of ​​about 2 cm × 2 cm) until the skin was completely exposed. Then, acetone and ether were mixed in equal volumes (1:1), and cotton dressings were soaked and gently applied to the exposed skin on the back of the neck of the mice for 15 seconds, and then distilled water cotton dressings were immediately applied for 30 seconds twice a day for 7 consecutive days, and the corresponding hemorrhoid gel was evenly applied (i.e., hemorrhoid gel DGJ1 was applied to experimental group 1, and hemorrhoid gel GJ1 was applied to experimental group 2). At the same time, the control group used the same operation, but only distilled water was applied for 45 seconds. Then, before applying the medicine on the 3rd, 5th and 7th days, the spontaneous itch activity of the experimental animals was recorded by video recording, and statistical analysis was performed. The specific results are shown in Table 5.

[0056] Table 5 Comparison of the number of scratching between the two groups ( ±s, times)

[0057] As can be seen from Table 5, compared with the control group, the scratching frequency of experimental group 1 and experimental group 2 was significantly reduced, indicating that both hemorrhoid gel GJ1 and hemorrhoid gel DGJ1 have antipruritic effects. Among them, the scratching frequency of experimental group 2 was significantly reduced compared with experimental group 1, indicating that the antipruritic effect of hemorrhoid gel GJ1 is more significant.

[0058] 5) Swelling and pain relief test 51) Experimental Materials Experimental animals: SPF grade male rats.

[0059] Reagent: Chloral hydrate.

[0060] Instrument: High frequency electrocautery therapy device.

[0061] 52) Animal modeling and experimental methods The anorectal model repair experiment was used to test the swelling and pain relief. Specifically, 15 SPF rats weighing 80-100g were randomly divided into three groups, with 5 rats in each group, namely experimental group 1, experimental group 2 and control group. After each group of rats was anesthetized with 10% chloral hydrate, a high-frequency electrocautery therapy device was used to create anorectal burn models. One day after modeling, the rats had redness and swelling around the anus and ulcers in the rectal mucosa. Experimental group 1 used a sterile cotton swab to dip hemorrhoid gel DGJ1 and evenly applied it to the perianal injury site. Experimental group 2 used a sterile cotton swab to dip hemorrhoid gel GJ1 and evenly applied it to the perianal injury site, twice a day, for 7 consecutive days. The control group used the same operation, but only applied it with distilled water. Before applying the medicine on the 3rd, 5th, and 7th days, photos were taken to observe the recovery of the rats' anorectum. The results are shown in Tables 6 and Figure 4 .

[0062] Table 6 Recovery of anorectal burns

[0063] As can be seen from Table 6, both experimental group 1 and experimental group 2 recovered in 6-7 days, while the control group needed 10-12 days to recover. It can be seen that experimental group 1 and experimental group 2 recovered faster than the control group, and the ulcer surface formed in the middle was smaller, indicating that hemorrhoid gel GJ1 and hemorrhoid gel DGJ1 both have the effect of reducing swelling and relieving pain. Among them, experimental group 2 recovered faster than experimental group 1, indicating that the effect of reducing swelling and relieving pain of hemorrhoid gel GJ1 is more significant.

[0064] In summary, it can be seen that the An Tai stock solution prepared using the formula of the present invention has good antibacterial, antipruritic, anti-swelling and analgesic effects, among which the An Tai stock solution prepared using modern biotechnology-enzymatic hydrolysis technology and ultrasonic technology has better effects. And the An Tai stock solution prepared using modern biotechnology-enzymatic hydrolysis technology and ultrasonic technology has a higher content of active ingredients and extraction rate than the traditional water extraction method.

[0065] The Antai stock solution prepared by the present invention can be used to prepare products of different dosage forms such as sanitary wet wipes, wet toilet paper, antibacterial sprays, antibacterial lotions or antibacterial gels.

[0066] Embodiment 5-8 Preparation method of Antai stock solution Examples 5 to 8 are respectively a method for preparing an Antai stock solution, and their steps are basically the same as the preparation steps of Antai stock solution G in Example 1, and the only difference is the difference in the amount of some raw materials and the process parameters, as shown in Table 7 for details: Table 7 List of process parameters in Examples 5 to 8

[0067] The contents of other parts of Examples 5 to 8 are the same as those of Example 1.

[0068] Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

Claims

1. A Chinese medicine composition of Antai stock solution, characterized in that: In terms of weight, the prescription of the An Tai original liquid Chinese medicine composition is: 5-15 parts of Phellodendron chinense, 3-12 parts of Sophora flavescens, 3-10 parts of Sophora japonica seeds, 3-10 parts of Honeysuckle, 1-5 parts of Sanguisorba officinalis, 3-10 parts of Imperata rhizome, 3-10 parts of Citrus aurantium, 1-5 parts of Panax notoginseng and 1-5 parts of Corydalis yanhusuo.

2. A method for preparing an Antai stock solution, characterized in that: The preparation method is to take the An Tai stock solution Chinese medicine composition of claim 1, and prepare the An Tai stock solution by soaking, enzymolysis and ultrasonic extraction; Alternatively, the preparation method is to take the An Tai stock solution Chinese medicine composition according to claim 1 and use a water extraction method to prepare the An Tai stock solution.

3. The method for preparing the Antai stock solution according to claim 2, characterized in that: The preparation method comprises the following steps: S1. Take Phellodendron amurense, Sophora flavescens, Sophora japonica seeds, Lonicera japonica, Sanguisorba officinalis, Imperata cylindrica, Fructus aurantii, Panax notoginseng and Corydalis yanhusuo, crush them, sieve them, soak them in water and obtain a soaked medicinal material system; Take cellulase, pectinase and hemicellulase to prepare a complex enzyme solution; S2, adding the complex enzyme solution to the soaked medicinal material system, performing enzymolysis, inactivating the enzyme, filtering, and obtaining a filtrate; S3, the filtrate is subjected to ultrasonic extraction, and the precipitate is separated by centrifugation to obtain a centrifuge; S4. The centrifuged liquid is sterilized to obtain the An Tai stock solution.

4. The method for preparing the Antai stock solution according to claim 3, characterized in that: In the complex enzyme solution, the weight ratio of cellulase, pectinase and hemicellulase is 2~4:2~4:1~2; The enzyme concentration in the complex enzyme solution is 0.1~1.0%; The pH value of the complex enzyme solution is 3.0~6.0; In the process of preparing the complex enzyme solution, the solvent used is sodium citrate-citric acid buffer, phosphate buffer, acetate buffer or disodium hydrogen phosphate-citric acid buffer.

5. The method for preparing the Antai stock solution according to any one of claims 2 to 4, characterized in that: The temperature of enzymatic hydrolysis is 30~66℃ and the time is 1~4h.

6. The method for preparing the Antai stock solution according to any one of claims 2 to 4, characterized in that: The temperature of ultrasonic extraction is 40~50℃, the frequency is 35~45kHz, and the time is 30~90min.

7. The method for preparing the Antai stock solution according to any one of claims 2 to 4, characterized in that: The soaking step is to add 8 to 13 times the weight of the prescribed amount of water and soak for 2 to 3 hours.

8. The method for preparing the Antai stock solution according to any one of claims 2 to 4, characterized in that: The An Tai stock solution is 5 to 10 times by weight of the prescribed amount.

9. The method for preparing the Antai stock solution according to claim 3 or 4, characterized in that: The sieve hole size is 12~24 mesh.

10. An application of Antai stock solution, characterized in that: The An Tai stock solution is made from the An Tai stock solution Chinese medicine composition according to claim 1, and the An Tai stock solution is used to prepare sanitary wet wipes, wet toilet paper, antibacterial spray, antibacterial lotion or antibacterial gel.

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