Skin care composition and product thereof

Abstract

The invention belongs to the technical field of skin care product compositions, particularly relates to a composition with a flower extract and a product, and more particularly relates to a composition with a natural flower extract and a product. The invention provides a skin care composition which comprises a flos magnoliae extract, a campsis grandiflora extract and any one of the following flower extracts: a peony flower extract or a peony flower extract. The applicant creatively discovers that the flower compositions have synergistic soothing and / or anti-allergy effects and can effectively soothe skin and improve sensitivity, and compared with existing chemically synthesized soothing agents, the composition is natural and is safer and more effective.

Description

Technical Field

[0001] The present invention belongs to the technical field of skin care compositions, and in particular, relates to a composition and a product having flower extracts, and more particularly, relates to a composition and a product having natural flower extracts. Background Art

[0002] Sensitive skin (SS) refers specifically to a highly reactive state of the skin under physiological or pathological conditions, mainly occurring on the face. Most people with sensitive skin have a normal appearance, but a few may have flaky or diffuse flushing, erythema, and capillary dilation on their face, which may be accompanied by dryness and fine scales. Usually, the skin will experience varying degrees of burning, stinging, itching, and tightness after being stimulated by physical, chemical, or mental factors. Current studies believe that the occurrence of sensitive skin is a complex process involving the skin barrier, neurovascular system, and immune inflammation. Under the interaction of internal and external factors, the skin barrier function is impaired, causing an increase in sensory nerve afferent signals, which leads to an increased reactivity of the skin to external stimuli and triggers an immune inflammatory response in the skin.

[0003] Sensitive skin has become a hot topic among the general public in my country. According to a recent survey of 20,000 Chinese female consumers, 32.8% of adult women have experienced skin sensitivity symptoms, and 52.2% of adult women have skin sensitivity symptoms on a regular basis. Among them, women with sensitive skin are mainly mildly sensitive, accounting for nearly 60%. Peeling, red blood streaks, and tightness are the most common symptoms for women with sensitive skin. Moderate to severe sensitivity also causes tingling, itching, burning, and other problems.

[0004] According to different skin types, sensitive skin can be divided into dry sensitive skin and oily sensitive skin, and the two have different sensitive symptoms; relatively speaking, dry sensitive skin is more prominent in tightness and peeling, while oily sensitive skin is more prominent in flushing, itching, and tingling. Nearly 60% of sensitive skin becomes sensitive due to irregular work and rest, environmental / temperature changes. In addition, excessive skin care, cosmetics abuse, and changing cosmetics are also important factors that induce sensitive skin.

[0005] At present, the cosmetics industry often takes care of sensitive skin through the following five ways. 1. Moisturizing, moisturizing agents: glycerin, hyaluronic acid, mushroom glucan, trehalose, etc.; auxiliary moisturizing agents: collagen, human collagen, avocado oil, etc.; 2. Soothing and anti-inflammatory: spring water (containing silicon dioxide, selenium, etc.), licorice, purslane, etc., glucocorticoids such as dexamethasone, etc., although they have strong soothing and anti-inflammatory effects, they are easy to cause hormone-dependent dermatitis and are prohibited in the cosmetics industry (if used, they are prohibited additions); 3. Reduce TRPV1 receptor activity: α-bisabolol, etc.; 4. Reduce vascular hyperreactivity: hydroxytyrosol, vitamin A aldehyde, Panax notoginseng (total saponins), chamomile, tea polyphenols, angelica, seaweed, etc.; 5. Repair skin barrier: ceramide, hyaluronic acid, squalene, etc.

[0006] Based on the above, it can be seen that most soothing substances in this field are synthetic products, and there is an urgent need for a product that uses natural formulation technology to safely and effectively soothe the skin and improve sensitive conditions. Summary of the invention

[0007] In view of this, in a first aspect, the present invention provides a skin care composition, comprising:

[0008] Primrose extract, Campsis grandiflora extract, and

[0009] Any of the following flower extracts:

[0010] Peony flower extract, or peony flower extract.

[0011] The applicant creatively discovered that the flower compositions of the present invention have synergistic soothing and / or anti-allergic effects with each other, and can effectively soothe the skin and improve sensitivity. Compared with existing chemical synthetic soothing agents, the composition of the present invention is derived from nature and is safer and more effective.

[0012] In some specific embodiments, the skin care composition of the present invention comprises:

[0013] Primrose extract, Campsis creeper extract, and Paeonia suffruticosa flower extract.

[0014] In some specific embodiments, the skin care composition of the present invention comprises:

[0015] Primrose extract, Campsis creeper extract, and Paeonia lactiflora flower extract.

[0016] Furthermore, the mass / volume concentration ratio of the Myristica fragrans extract: the Trumpet creeper extract: the Paeonia suffruticosa extract is (1-4): (1-4): (1-4). Furthermore, the mass / volume concentration ratio of the Myristica fragrans extract: the Trumpet creeper extract: the Paeonia suffruticosa extract is 1:1:1.

[0017] Furthermore, the mass / volume concentration ratio of the Grandis Flos Bicolor extract: the Trumpet Creeper extract: the Paeonia lactiflora extract is (1-4): (1-4): (1-4). Furthermore, the mass / volume concentration ratio of the Grandis Flos Bicolor extract: the Trumpet Creeper extract: the Paeonia lactiflora extract is 1:1:1.

[0018] The composition configured in the above ratio can achieve better soothing and / or anti-sensitivity effects.

[0019] Furthermore, the concentration of the Primrose extract in the composition is 1 to 20 mg / mL, specifically, for example, 1 mg / mL, 2 mg / mL, 3 mg / mL, 4 mg / mL, 5 mg / mL, 10 mg / mL, 15 mg / mL, 20 mg / mL, preferably 1 to 10 mg / mL, more preferably 1 to 5 mg / mL.

[0020] Furthermore, the concentration of the trumpet creeper extract in the composition is 1 to 20 mg / mL, specifically, for example, 1 mg / mL, 2 mg / mL, 3 mg / mL, 4 mg / mL, 5 mg / mL, 10 mg / mL, 15 mg / mL, 20 mg / mL, preferably 1 to 10 mg / mL, more preferably 1 to 5 mg / mL.

[0021] Furthermore, the concentration of the peony extract in the composition is 1 to 20 mg / mL, specifically, for example, 1 mg / mL, 2 mg / mL, 3 mg / mL, 4 mg / mL, 5 mg / mL, 10 mg / mL, 15 mg / mL, 20 mg / mL, preferably 1 to 10 mg / mL, more preferably 1 to 5 mg / mL.

[0022] Furthermore, the concentration of the peony flower extract in the composition is 1 to 20 mg / mL, specifically, for example, 1 mg / mL, 2 mg / mL, 3 mg / mL, 4 mg / mL, 5 mg / mL, 10 mg / mL, 15 mg / mL, 20 mg / mL, preferably 1 to 10 mg / mL, more preferably 1 to 5 mg / mL.

[0023] Furthermore, the extract is an alcohol extract, a water extract, or a carbon dioxide supercritical extract; further, the alcohol extract is an ethanol extract, a 1,3-butanediol extract, or a 1,3-propylene glycol extract.

[0024] In a second aspect, the present invention provides use of the above composition for preparing a product with soothing and / or anti-sensitivity effects.

[0025] Furthermore, the present invention provides use of the composition of the present invention in soothing and / or anti-sensitivity.

[0026] In a third aspect, the present invention provides a product with soothing and / or anti-sensitivity effects, comprising the composition as described above.

[0027] Furthermore, the product may be a skin care product or a cosmetic.

[0028] Furthermore, the cosmetics may be makeup.

[0029] Furthermore, the mass concentration or mass volume concentration of the composition in skin care products or cosmetics is 0.001-5%.

[0030] Specifically, the mass concentration or mass volume concentration can be 0.001-5%, 0.001-3%, 0.001-1%, 0.001-0.5%, 0.001-0.1%, 0.01-5%, 0.01-3%, 0.01-1%, 0.01-0.5%, 0.01-0.1%, etc.

[0031] Furthermore, the final concentration of the Myrica dahurica extract in skin care products or cosmetics is 0.5-500 μg / ml, preferably 0.5-250 μg / ml, preferably 50-250 μg / ml, more preferably 50 μg / ml.

[0032] Furthermore, the final concentration of the Trumpet Creeper extract in skin care products or cosmetics is 0.5-500 μg / ml, preferably 0.5-250 μg / ml, preferably 50-250 μg / ml, more preferably 50 μg / ml.

[0033] Furthermore, the final concentration of the peony flower extract in the skin care product or cosmetics is 0.5-500 μg / ml, preferably, 0.5-250 μg / ml, preferably, 50-250 μg / ml, more preferably, 50 μg / ml.

[0034] Furthermore, the products include lipstick, lip gloss, loose powder, pressed powder, primer, foundation, blush, contouring, eye shadow, makeup remover, cleanser, essence, lotion, emulsion and cream.

[0035] For example, the mass volume concentration of the composition in lipstick, lip gloss, loose powder, pressed powder, makeup base, foundation, blush, contouring, eye shadow, makeup remover, and cleanser is 0.001-0.01%, that is, 0.001-0.01 g of the composition is added to every 100 mL, or the mass concentration of the composition is 0.001-0.01%.

[0036] For example, the mass volume concentration of the composition in essence, lotion, emulsion, and cream is 0.01 to 0.05%, that is, 0.01 to 0.05 g of the composition is added per 100 mL, or the mass concentration of the composition is 0.01 to 0.05%.

[0037] Furthermore, the product also includes one or more of an emulsifier, a surfactant, a carrier, a flavor, a preservative, and an antioxidant.

[0038] In some specific embodiments, the product of the present invention also includes one or more of isopropyl lauroyl sarcosine, water, cetearyl alcohol, stearic acid, EFA oil, C12-15 alcohol benzoate, isohexadecane, ethylhexylglycerin, dimethicone, dipotassium glycyrrhizate, allantoin, potassium lauryl phosphate, C12-16 alkyl glucoside, disodium EDTA, 1,3-propylene glycol, glycerin, xanthan gum, chlorphenesin / phenoxyethanol, and fragrance.

[0039] In a specific embodiment, the product of the present invention further comprises one or more of water, lactic acid, PEG-25 castor oil, 1,3-propylene glycol, ethanol, menthol, preservatives, and flavors.

[0040] In a specific embodiment, the product of the present invention further comprises one or more of talc, titanium dioxide, silica, yellow iron oxide, red iron oxide, black iron oxide, zinc stearate, acetylated lanolin, polydimethylsiloxane, preservatives, and flavors. BRIEF DESCRIPTION OF THE DRAWINGS

[0041] Figure 1 The results of the zebrafish soothing test on Flos Ampelopsis-Campsis Creeper-Peony;

[0042] Figure 2 The zebrafish soothing test results of Flos Ampelopsis-Campsis Creeper-Peony;

[0043] Figure 3 This is the result of the zebrafish soothing test for the control group;

[0044] Figure 4 The negative control was 0.9% NaCl, and the positive controls were 0.2%, 0.3%, and 0.5% sodium hydroxide solution CAM test results;

[0045] Figure 5 This is the CAM test result of the formula of Flos Forsythias-Campsis Creepers-Peony Flower Extract;

[0046] Figure 6 These are the CAM test results for the formula of extracts from Flos Formosans-Camellias oleifera-Peony odoratus. DETAILED DESCRIPTION

[0047] The present invention will be described in detail below in conjunction with specific embodiments and examples, and the advantages and various effects of the present invention will be more clearly presented. It should be understood by those skilled in the art that these specific embodiments and examples are used to illustrate the present invention, rather than to limit the present invention.

[0048] The term "Magnolia biondii and Magnolia biondii extract" refers to the dried flower buds of Magnolia biondii Pamp., also known as magnolia, which is a plant of the Magnoliaceae family. my country's cosmetics industry can use Magnolia biondii (MAGNOLIA BIONDII) bud / flower extracts and Magnolia biondii (MAGNOLIABIONDII) flower extracts as cosmetic raw materials for the development and application of cosmetic formulas. Magnolia biondii contains chemical components such as lignans, flavonoids, alkaloids and volatile oils, and can be prepared by conventional extraction methods such as hydroalcohol extraction, 1,3-propylene glycol or 1,3-butylene glycol extraction, or supercritical CO2, microwave and ultrasonic extraction, etc., or commercially available raw materials can also be used.

[0049] The term "campsis grandiflora and campsis radicans extract" refers to the dried flowers of Campsis grandiflora (Thunb.) K.Schum. or Campsis radicans (L.) Seem., plants of the Bignoniaceae family. my country's cosmetics industry can use Campsis grandiflora flower extract or Campsis radicans flower extract as a cosmetic raw material for the development and application of cosmetic formulas. Campsis grandiflora flower extract or Campsis radicans flower extract contains triterpenes, flavonoids, phenylpropanoid glycosides, iridoid glycosides, volatile oils and other chemical components, and can be prepared by conventional extraction methods such as hydroalcohol extraction, 1,3-propylene glycol or 1,3-butylene glycol extraction, or supercritical CO2, microwave and ultrasonic extraction, etc., or commercially available raw materials can also be used.

[0050] The term "peony flower and peony flower extract" refers to the dried flowers or buds of Paeonia suffruticosa Andr., a plant of the Ranunculaceae family. In my country's cosmetics industry, peony (PAEONIA SUFFRUTICOSA) flower / leaf / root extract, peony (PAEONIA SUFFRUTICOSA) flower water and peony (PAEONIA SUFFRUTICOSA) branch / flower / leaf extract can be used as cosmetic raw materials for the development and application of cosmetic formulas. Peony flowers contain chemical components such as flavonoids and polyphenols, which can be prepared according to conventional extraction methods such as water-alcohol extraction, 1,3-propylene glycol or 1,3-butylene glycol extraction, or supercritical CO2, microwave and ultrasonic extraction, etc., or commercially available raw materials can also be used.

[0051] The term "peony flower and peony flower extract" refers to the dried flowers or buds of Paeonia lactiflora Pall., a plant of the Ranunculaceae family. In my country's cosmetics industry, peony (PAEONIAALBIFLORA) flower extract and medicinal peony (PAEONIAOFFICINALIS) flower extract can be used as cosmetic raw materials for the development and application of cosmetic formulas. Peony flowers contain flavonoids, phenylpropanoids, acetophenones, as well as chemical components such as volatile oils, fatty acids, and sterols. They can be prepared by conventional extraction methods such as hydroalcohol extraction, 1,3-propylene glycol or 1,3-butylene glycol extraction, or supercritical CO2, microwave and ultrasonic extraction, etc., or commercially available raw materials can be used.

[0052] Specifically, for example:

[0053] 1. Preparation process of ethanol extract of Flos Ampelopsis

[0054] Weigh 1748 g of the medicinal material Forsythia suspensa, add 8 times the volume of the Forsythia suspensa (8740 mL) of 80% ethanol by volume for ultrasonic or microwave extraction, the extraction temperature is 80°C, the first extraction time is 3 hours, after 3 hours, add 50 g of activated carbon and stir evenly, heat in a water bath at 80°C for 60 min to decolorize, filter while hot, cool the filtrate to 60°C and vacuum filter to obtain an extract.

[0055] Preparation of diatomaceous earth filter cake: spread diatomaceous earth 5% + EDTANa 21% in a beaker, stir evenly with pure water and then vacuum filter to obtain diatomaceous earth filter cake, then wash the diatomaceous earth filter cake with 500 ml of pure water to remove metal ions and reduce conductivity, and set aside.

[0056] The extract of step 1 is vacuum filtered using the diatomaceous earth filter cake prepared above, and the filtrate is concentrated under reduced pressure to recover ethanol to obtain a first concentrated solution.

[0057] · Second extraction, the residue of the Flos Miscanthus remaining after the first extraction was extracted again by the above method. After 2 hours of extraction, 50g of activated carbon was added and stirred evenly. The mixture was decolorized after heating in a water bath at 80℃ for 60min and filtered while hot. The filtrate was cooled to 60℃ and vacuum filtered. The filtrate was combined with the first concentrated solution and concentrated under reduced pressure to recover ethanol. The extract was concentrated to an extract and weighed.

[0058] Weigh the above extract and prepare (2% extract by volume, 28% deionized water by volume, 70% 1,3-propylene glycol by volume) and stir to dissolve, add 10% of the above volume of diatomaceous earth by mass and stir for 2 hours and let stand overnight. After overnight, use the prepared diatomaceous earth filter cake for vacuum filtration, add 1,3-propylene glycol to the sample solution after filtration to 100% of the total weight, and stir until clear and transparent. Heat sterilize at 80℃ for 2 hours and cool to 40℃ for filling.

[0059] 2. Trumpet creeper ethanol extract - preparation process

[0060] Weigh 500 g of the medicinal material Trumpet Creeper, add 8 times the volume of the Trumpet Creeper (4000 mL) with a volume concentration of 80% ethanol for ultrasonic or microwave extraction, the extraction temperature is 80°C, the first extraction time is 3 hours, 100 g of activated carbon is added after 3 hours and stirred evenly, decolorize after heating in a water bath at 80°C for 60 minutes, filter while hot, cool the filtrate to 60°C and vacuum filter to obtain an extract.

[0061] Preparation of diatomaceous earth filter cake: spread diatomaceous earth 5% + EDTANa 21% in a beaker, stir evenly with pure water and then vacuum filter to obtain diatomaceous earth filter cake, then wash the diatomaceous earth filter cake with 500 ml of pure water to remove metal ions and reduce conductivity, and set aside.

[0062] The extract of step 1 was added with a volume of 8 times the mass of Flos Maydens (2400 mL) to a concentration of , and the filtrate was concentrated under reduced pressure to recover ethanol, thereby obtaining a first concentrated solution.

[0063] · Second extraction, the remaining trumpet creeper residue after the first extraction is extracted again by the above method. After extraction for 2 hours, 100g of activated carbon is added and stirred evenly. After heating in a water bath at 80℃ for 60min, it is decolorized and filtered while hot. The filtrate is cooled to 60℃ and vacuum filtered. The filtrate is combined with the first concentrated solution and concentrated under reduced pressure to recover ethanol. The extract is concentrated to an extract and weighed.

[0064] Weigh the above extract and prepare (5% extract by volume, 25% deionized water by volume, 70% 1,3-propylene glycol by volume) and stir to dissolve, add 10% of the total volume of diatomaceous earth by mass and stir for 2 hours and let stand overnight. After overnight, use the prepared diatomaceous earth filter cake for vacuum filtration, add 1,3-propylene glycol to the sample solution after filtration to 100% of the total weight, and stir until clear and transparent. Heat sterilize at 80℃ for 2 hours and cool to 40℃ for filling.

[0065] 3. Peony ethanol extract - preparation process

[0066] Weigh 500 g of the medicinal material peony flower, add 8 times the volume of the peony flower (4000 mL) with a volume concentration of 80% ethanol for ultrasonic or microwave extraction, the extraction temperature is 80°C, the first extraction time is 3 hours, 80 g of activated carbon is added after 3 hours and stirred evenly, decolorize after heating in a water bath at 80°C for 60 minutes, filter while hot, cool the filtrate to 60°C and vacuum filter to obtain an extract.

[0067] Preparation of diatomaceous earth filter cake: spread diatomaceous earth 5% + EDTANa 21% in a beaker, stir evenly with pure water and then vacuum filter to obtain diatomaceous earth filter cake, then wash the diatomaceous earth filter cake with 500 ml of pure water to remove metal ions and reduce conductivity, and set aside.

[0068] The extract of step 1 is vacuum filtered using the diatomaceous earth filter cake prepared above, and the filtrate is concentrated under reduced pressure to recover ethanol to obtain a first concentrated solution.

[0069] · Second extraction, the peony flower residue remaining after the first extraction is extracted again by the above method, after extraction for 2 hours, 70g of activated carbon is added and stirred evenly, and the mixture is decolorized after heating in a water bath at 80°C for 60min, filtered while hot, and the filtrate is cooled to 60°C and vacuum filtered, the filtrate is combined with the first concentrated solution and concentrated under reduced pressure to recover ethanol, and the extract is concentrated to an extract and weighed.

[0070] Weigh the above extract and prepare (3% extract by volume, 27% deionized water by volume, 70% 1,3-propylene glycol by volume) and stir to dissolve, add 10% of the total volume of diatomaceous earth by mass and stir for 2 hours until overnight. After overnight, use the prepared diatomaceous earth filter cake for vacuum filtration, add 1,3-propylene glycol to the sample solution after filtration to 100% of the total weight, and stir until clear and transparent. Heat sterilize at 80℃ for 2 hours and cool to 40℃ for filling.

[0071] 4. Preparation process of peony flower ethanol extract

[0072] Weigh 500 g of the medicinal materials and peony flowers, add 8 times the volume of the peony flowers (4000 mL) with a volume concentration of 80% ethanol for ultrasonic or microwave extraction, the extraction temperature is 80°C, the first extraction time is 3 hours, after 3 hours, add 80 g of activated carbon and stir evenly, heat in a water bath at 80°C for 60 min to decolorize, filter while hot, cool the filtrate to 60°C and vacuum filter to obtain an extract.

[0073] Preparation of diatomaceous earth filter cake: spread diatomaceous earth 5% + EDTANa 21% in a beaker, stir evenly with pure water and then vacuum filter to obtain diatomaceous earth filter cake, then wash the diatomaceous earth filter cake with 500 ml of pure water to remove metal ions and reduce conductivity, and set aside.

[0074] The extract of step 1 is vacuum filtered using the diatomaceous earth filter cake prepared above, and the filtrate is concentrated under reduced pressure to recover ethanol to obtain a first concentrated solution.

[0075] · Second extraction, the peony flower residue remaining after the first extraction is extracted again by the above method. After extraction for 2 hours, 70g of activated carbon is added and stirred evenly. After heating in a water bath at 80°C for 60min, decolorization is performed and filtered while hot. The filtrate is cooled to 60°C and vacuum filtered. The filtrate is combined with the first concentrated solution and concentrated under reduced pressure to recover ethanol. The extract is concentrated to an extract and weighed.

[0076] Weigh the above extract and prepare (3% extract by volume, 27% deionized water by volume, 70% 1,3-propylene glycol by volume) and stir to dissolve, add 10% of the total volume of diatomaceous earth by mass and stir for 2 hours until overnight. After overnight, use the prepared diatomaceous earth filter cake for vacuum filtration, add 1,3-propylene glycol to the sample solution after filtration to 100% of the total weight, and stir until clear and transparent. Heat sterilize at 80℃ for 2 hours and cool to 40℃ for filling.

[0077] Example 1. Comparison of the soothing effects of the compositions of the present invention

[0078] 1) Cell plating: Dilute the mouse mononuclear macrophage leukemia cell line RAW264.7 cell suspension to a density of 2×10 5 Each well was inoculated into a 96-well plate at 100 μL / well and placed in a 37°C, 5% CO2 incubator for 24 h.

[0079] Note: Each test includes a blank control group, a model group, a test sample group and a positive control group, and three replicate wells are set up in each group.

[0080] 2) Sample addition: After culturing for 24 hours, carefully discard the culture supernatant and set up the following groups: ① Blank group: 500 μL complete culture medium; ② LPS group: LPS with a final concentration of 1 μg / mL; ③ Experimental group: different concentrations of samples and LPS with a final concentration of 1 μg / mL; ④ Positive control group: dexamethasone and various control materials, and LPS with a final concentration of 1 μg / mL; incubate in an incubator for 24 hours.

[0081] Note: The blank control group only contained cell culture medium, and the positive control group contained cell culture medium containing positive substances.

[0082] 3) Collection of cell supernatant: After the incubation is completed, the supernatant is collected.

[0083] 4) Detection: Detect the NO content according to the kit instructions.

[0084] Calculate NO inhibition rate (%) = (1-T / C) × 100%

[0085] T—the average of three times of NO content in the test group;

[0086] C—average of 3 times of NO content in negative control group. The results are shown in Table 1 below (the experimental concentration of the composition of the present invention is designed according to the cell safety test data). As can be seen from Table 1, the combination of the three-group prescription of Flos Myrtillus extract + Flos Campsis extract + (Peony flower extract or Paeonia lactiflora extract) is much better than the combination of the two and the three alone, and the combination of the three prescriptions is higher than the sum of the inhibitory effects of the three alone, which illustrates the synergistic effect of the three prescriptions of the present invention.

[0087] In addition, the commonly used soothing components purslane, licorice, chamomile and madecassoside in the art are used to replace the forsythia extract. However, the introduction of these components does not significantly increase the soothing effects of the other two components, further indicating the synergistic effect of the three components of the composition of the present invention.

[0088] Table 1

[0089]

[0090] Example 2: In vitro soothing test of the composition of the present invention

[0091] Experimental Principle

[0092] Cosmetics with soothing effects can help improve skin irritation. The occurrence of skin irritation mainly involves the physiological process of skin barrier-neurovascular-immune inflammation. Skin irritation problems can be improved by soothing skin inflammatory reactions. Nitric oxide (NO) participates in the physiological process of skin inflammatory reactions as an immune regulatory factor. By inhibiting the release of excessive nitric oxide (NO) by activated immune cells, the skin's immune response can be reduced, thereby improving the skin's irritation.

[0093] Materials and methods

[0094] Experimental cells: Mouse mononuclear macrophage leukemia cell line RAW264.7.

[0095] Experimental method: Sample cytotoxicity experiment and efficacy evaluation test were carried out according to the "QYGEMG-SOP-2002RAW264.7 Macrophage Soothing Efficacy Determination Operating Procedure" of the Basic Research Department.

[0096] Cytotoxicity assay

[0097] 1) Cell plating: dilute the cell suspension to a density of 2×10 5 Each well was inoculated into a 96-well plate at 100 μL / well and placed in a 37°C, 5% CO2 incubator for 24 h.

[0098] 2) Sample addition: Take out the 96-well plate, discard the supernatant, add DMEM complete medium to the normal control group, add DMEM complete medium containing samples of different concentrations to the sample group, 100 μL / well, and culture in a 37°C, 5% CO2 incubator for 24 h;

[0099] 3) Cell viability detection: Take out the 96-well plate, place it for about 10 minutes to balance, add CCK8 detection reagent, 10 μL / well, incubate at 37°C for 1 hour, and then detect the OD value; calculate cell viability:

[0100] Cell viability (%) = sample OD value / blank OD value * 100%

[0101] Note: Subsequent efficacy evaluation tests were performed when the cell viability was above 90%.

[0102] Efficacy evaluation

[0103] 1) Cell plating: same as above;

[0104] Note: Each test includes a blank control group, a model group, a test sample group and a positive control group, and three replicate wells are set up in each group.

[0105] 2) Sample addition: After culturing for 24 hours, carefully discard the culture supernatant and set up the following groups: ① Blank group: 500 μL complete culture medium; ② LPS group: LPS with a final concentration of 1 μg / mL; ③ Experimental group: different concentrations of samples and LPS with a final concentration of 1 μg / mL; ④ Positive control group: dexamethasone and various control materials, and LPS with a final concentration of 1 μg / mL; incubate in an incubator for 24 hours.

[0106] Note: The blank control group only contained cell culture medium, and the positive control group contained cell culture medium containing positive substances.

[0107] 3) Collection of cell supernatant: After the incubation is completed, the supernatant is collected.

[0108] 4) Detection: Detect the NO content according to the kit instructions.

[0109] The mean value (Mean) and standard deviation (SD) of each group of experiments were calculated, and Graphpad Prism software was used for data statistical analysis. One-way Anova analysis was used for data between multiple groups. P < 0.05 was considered a significant difference, P < 0.01 was considered an extremely significant difference, and P > 0.05 was considered to be insignificant. The results are shown in Table 2.

[0110] Table 2

[0111]

[0112] Example 3: Zebrafish soothing efficacy test of the composition of the present invention

[0113] Method source - "T / ZHCA016-2022 Evaluation of the soothing effect of cosmetics - Neutrophil inhibition rate method for zebrafish larvae"

[0114] System and sample size

[0115] Experimental system: Transgenic neutrophil green fluorescence zebrafish (MPX). Zebrafish age: 2 days post fertilization (2dpf). Experimental sample size per group: 15 fish (N=10).

[0116] Adult fish rearing and breeding methods: follow the standard rearing and breeding methods of our laboratory, in line with the requirements of the international AAALAC certification (certification number: 001458).

[0117] Principle and method

[0118] Sodium dodecyl sulfate (SLS) may cause irritation in the body after acting on it. The irritant enters the zebrafish body, induces an inflammatory response, and neutrophils have an immune response, migrating to the skin epidermis and gathering. The changes in the number of neutrophils in the skin of transgenic neutrophil green fluorescent strain zebrafish (MPX) before and after treatment were used to detect whether the sample has a soothing effect.

[0119] Experimental procedures

[0120] 1) Randomly select zebrafish and place them in a 6-well plate, with 15 zebrafish in each well.

[0121] 2) The zebrafish skin inflammation model was established by administering SLS in water.

[0122] 3) Water-soluble samples were administered, and a normal control group and a model control group were set up at the same time. The volume of each well was 3 mL.

[0123] 4) Incubate at 28°C in the dark for 18 h.

[0124] 5) Ten zebrafish were randomly selected from each experimental group and photographed under a fluorescence microscope. Advanced image processing software was used to analyze and collect data, and the number of neutrophils in the zebrafish skin (N) was analyzed. The formula was used to calculate and determine whether the sample had a soothing effect.

[0125] Soothing effect (%) = 100% × [N (model control group) - N (sample group)] / [N (normal control group) - N (model control group)]

[0126] Judgment basis: Statistical analysis p < 0.05, judged to be significant difference. The results are shown in Table 3 and Figures 1 to 3 shown.

[0127] Table 3

[0128]

[0129]

[0130] Example 4: Safety test of the composition of the present invention

[0131] 1. Experimental materials: SPF-grade fertilized chicken embryos of Bailaihang chicken, sodium chloride (Solarbio), sodium hydroxide (Shanghai McLean Co., Ltd.);

[0132] 2. Experimental method: Based on SN / T2329-2009 "Chicken embryo chorioallantoic membrane test for eye irritation / corrosion of cosmetics";

[0133] 3. Sample preparation: Use an analytical balance to accurately weigh 1:1:1 ratio of Flos Formosans Wollennicornii extract, ...

[0134] Test results:

[0135] Through the endpoint evaluation method, 0.3 ml of the sample was directly dropped on the CAM. After 3 minutes of action, the test substance on the CAM membrane was gently rinsed with physiological saline to observe the bleeding, coagulation, and vascular dissolution, and photographed. At the same time, a negative control of 0.9% NaCl and a positive control of 0.2%, 0.3%, and 0.5% sodium hydroxide solution were set. The results are shown below. Figures 4 to 6 As shown in the figure, it can be seen that after 3 minutes of action of the composition of the present invention [5% (v / v)], no obvious bleeding was observed in the CAM compared with the negative control group, so the sample was non-irritating under the test conditions.

[0136] Example 5: Preparation of soothing skin care products using the composition of the present invention

[0137] Skin care products, facial cleanser and skin cleanser, were prepared according to the formulas in Table 4 and Table 5 and the method described below.

[0138] Cleanser:

[0139] (1) Add phase A to an oil pan, heat and stir until the temperature reaches 80-85°C;

[0140] (2) Add phase B1 into a water pot, stir and heat to 80-80°C, add phase B2, stir well, and keep warm for 20 minutes;

[0141] (3) Add phase B to the homogenizer, add phase A, stir and emulsify for 5 minutes, and homogenize for 3 minutes;

[0142] (4) When the temperature drops to 45°C, add phase C and mix well, then cool to 36°C.

[0143] Cleansing water:

[0144] (1) Heat the raw materials of phase A to 80-85°C, stir to dissolve and mix, and keep warm for 10 minutes;

[0145] (2) Stir and dissolve all the ingredients in phase B, then slowly add phase B into phase A and stir to mix;

[0146] (3) Premix the components of phase C, add a small amount of ethanol to help dissolve, and then add dropwise to the mixed phase AB;

[0147] (4) Stir and mix, cool to room temperature, and filter through a filter cloth with a mesh size of 400 or above to obtain the product.

[0148] Table 4

[0149]

[0150]

[0151] Table 5

[0152]

[0153] Prepare a powder cake according to the formula in Table 6 below and the method described:

[0154] (1) Mix phase A in a ribbon mixer for about 1 h;

[0155] (2) Add the pre-mixed phase C into the mixer, mix for about 2 hours, grind with a pulverizer, and pass through a 40-45 mesh sieve;

[0156] (3) Place the sieved powder in a ribbon mixer and spray the pre-melted liquid phase B while stirring. Stir for 5 hours and spray the flavor after cooling.

[0157] (4) granulated by a granulator, then passed through a pulverizer, and passed through a 25-mesh sieve;

[0158] (5) After cooling, pass through a 40-mesh sieve. During the sieving process, ensure that no heat is generated and the temperature does not rise to avoid loss of flavor volatilization;

[0159] (6) Finally, the obtained powder is filled into a container and pressed into shape. Generally, a pressure of 300 kPa is applied to press the powder into a powder cake.

[0160] Table 6

[0161]

[0162]

Claims

1. A skin care composition comprising: Primrose extract, Campsis grandiflora extract, and Any of the following flower extracts: Peony flower extract, or peony flower extract.

2. The composition according to claim 1, characterized in that The mass / volume concentration ratio of the Flos Bilobae extract: the Flos Campsis extract: the Flos Paeoniae Alba extract or the Flos Paeoniae Alba extract is (1-4): (1-4): (1-4).

3. The composition according to claim 2, characterized in that The mass / volume concentration ratio of the Flos Bilobae extract: the Flos Campsis extract: the Flos Paeoniae Suffruticosae extract is 1:1:1; or The concentration ratio of the Flos Bilobae extract: the Flos Campsis extract: the Flos Paeoniae Alba extract is 1:1:

1.

4. Use of the composition according to any one of claims 1 to 3 for soothing and / or anti-sensitivity effects.

5. A product with soothing and / or anti-allergic effects, comprising the composition according to any one of claims 1 to 3.

6. The product according to claim 5, characterized in that The product is a skin care product or a cosmetic.

7. The product according to claim 6, characterized in that The composition according to any one of claims 1 to 3 has a mass concentration or mass volume concentration of 0.001 to 5% in skin care products or cosmetics.

8. The product according to claim 6, characterized in that The final concentration of the extract of Flos Bilobae, the extract of Flos Campsis and the extract of Flos Paeoniae Alba in skin care products or cosmetics is 0.5 to 500 μg / ml, preferably 0.5 to 250 μg / ml.

9. The product according to any one of claims 6 to 8, characterized in that The product also includes one or more of an emulsifier, a surfactant, a carrier, a flavor, a preservative, and an antioxidant.

10. The product according to claim 9, characterized in that The soothing and / or anti-sensitivity products include any of the following formulas: Isopropyl lauroyl sarcosinate, water, cetearyl alcohol, stearic acid, EFA oil, C12-15 alkyl benzoate, isohexadecane, ethylhexylglycerin, dimethicone, dipotassium glycyrrhizate, allantoin, potassium lauryl phosphate, C12-16 alkyl glucoside, disodium EDTA, 1,3-propylene glycol, glycerin, xanthan gum, chlorphenesin / phenoxyethanol, and one or more of fragrance; One or more of water, lactic acid, PEG-25 castor oil, 1,3-propylene glycol, ethanol, menthol, preservatives, and flavors; or One or more of talc, titanium dioxide, silica, yellow iron oxide, red iron oxide, black iron oxide, zinc stearate, acetylated lanolin, polydimethylsiloxane, preservatives and flavors.

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