Barrier repair composition applicable to dry sensitive skin and application thereof

Abstract

The invention discloses a barrier repair composition applicable to dry sensitive skin and application thereof. The composition comprises a golden camellia extract, a loquat leaf extract, an alfalfa extract and hydrolyzed royal jelly protein. According to the barrier repairing composition applicable to the dry sensitive skin, disclosed by the invention, through reasonable matching and synergistic interaction of all the components, a better skin barrier repairing effect can be achieved, so that the problems of dry and tight skin, desquamation, erythema, itching, dull luster, redness, burning heat sensation, pricking pain and the like of the dry sensitive skin are fundamentally solved.

Description

Technical Field

[0001] The present invention relates to the field of cosmetics, and in particular to a barrier repair composition suitable for dry and sensitive skin and its application. Background Art

[0002] Dry and sensitive skin is a skin type with damaged skin barrier, low water content in the stratum corneum and prone to being stimulated to produce allergic reactions. With the acceleration of the industrialization process and the transformation of urban lifestyle, factors such as air pollution, increased ultraviolet exposure, and drastic changes in indoor and outdoor environmental humidity (such as low humidity caused by air-conditioning environment) have led to a significant upward trend in the incidence of dry and sensitive skin. According to statistics, the proportion of the global population with dry and sensitive skin has increased from 18% to 25% in the past five years, and the incidence rate in autumn and winter in northern China is as high as 32%. The typical symptoms of dry and sensitive skin include: dry and tight skin, desquamation, erythema, itching, dullness, etc., and there may also be discomfort such as flushing, burning sensation, stinging sensation, etc. In severe cases, contact dermatitis or atopic dermatitis may be induced.

[0003] Traditional skin care products will add various ingredients with functions such as skin moisturization, anti-allergy and soothing, etc. However, they only achieve a soothing effect by increasing the water content of the skin, and it is difficult to fundamentally solve the problems of dry and sensitive skin. Antihistamine anti-allergy agents can only simply cut off the allergic reaction, and do not repair the skin barrier function. Moreover, their anti-allergy effect is average, and long-term use will also produce dependence and cause some other skin problems. In addition, products with skin barrier repair functions can be used to repair damaged skin barriers, such as hyaluronic acid, ceramide, cholesterol, fatty acids, etc. For example, Chinese Patent Application No. 201910602114.6 discloses a skin care product for soothing and repairing sensitive skin, including ceramide complex, skin conditioner, plant-derived emollient, low-molecular-weight hyaluronic acid, etc. Exogenous supplementation of lipids such as ceramide and cholesterol helps to improve and restore the damaged skin barrier function. Low-molecular-weight hyaluronic acid can be absorbed into the skin to play a better moisturizing and barrier repair function, but the improvement effect of this product on the skin problems of the subjects still needs to be improved.

[0004] With the increasing application of plant raw materials in cosmetics, more and more plant extracts with soothing and repairing ingredients are used to solve problems such as skin sensitivity and damaged skin barrier, including purslane, centella asiatica, chamomile, sophora flavescens, licorice, etc. However, the mechanism of action of plant extracts is not yet clear and further in-depth research is needed. Using the above plant extracts alone has no obvious effect. At present, most products containing the above plant extracts have acceptable effects on general skin, but when used for dry and sensitive skin, they cannot effectively improve the skin quality. Therefore, there is an urgent need to provide a skin barrier repair composition suitable for dry and sensitive skin, which can effectively solve the problems of dry and sensitive skin. Summary of the Invention

[0005] To solve the above problems, the present invention provides a barrier repair composition suitable for dry and sensitive skin and its application. The barrier repair composition suitable for dry and sensitive skin of the present invention can effectively solve problems such as dryness and tightness, desquamation, erythema, itching, lack of luster, flushing, burning sensation, and stinging sensation of dry and sensitive skin.

[0006] The technical solutions adopted by the present invention are as follows:

[0007] In a first aspect of the present invention, there is provided a barrier repair composition suitable for dry and sensitive skin, the composition comprising camellia nitidissima extract, eriobotrya japonica leaf extract, medicago sativa extract, and hydrolyzed royal jelly protein.

[0008] For the barrier repair composition suitable for dry and sensitive skin, wherein the weight ratio of the camellia nitidissima extract, the eriobotrya japonica leaf extract, the medicago sativa extract, and the hydrolyzed royal jelly protein is (5-12):(10-16):(13-20):(8-15).

[0009] For the barrier repair composition suitable for dry and sensitive skin, wherein the weight ratio of the camellia nitidissima extract, the eriobotrya japonica leaf extract, the medicago sativa extract, and the hydrolyzed royal jelly protein is 9:14:16:11.

[0010] Among them, the preparation method of the camellia nitidissima extract includes the following steps: taking camellia nitidissima flower powder and mixing it with an ethanol aqueous solution with a volume fraction of 50-70%, extracting 2-3 times at 70-80°C, each extraction for 1-2 hours, combining the extraction solutions, filtering, concentrating, and freeze-drying to obtain the camellia nitidissima extract. The material-liquid ratio of the camellia nitidissima flower powder to the ethanol aqueous solution is 1:(6-8).

[0011] The preparation method of the eriobotrya japonica leaf extract includes the following steps: taking eriobotrya japonica leaf powder and mixing it with an ethanol aqueous solution with a volume fraction of 60-80%, extracting 2-3 times at 70-80°C, each extraction for 1-2 hours, combining the extraction solutions, filtering, concentrating, and freeze-drying to obtain the eriobotrya japonica leaf extract. The material-liquid ratio of the eriobotrya japonica leaf powder to the ethanol aqueous solution is 1:(5-9).

[0012] The preparation method of the medicago sativa extract includes the following steps: taking medicago sativa powder and mixing it with an ethanol aqueous solution with a volume fraction of 70-90%, extracting 2-3 times at 70-80°C, each extraction for 0.5-1 hour, combining the extraction solutions, filtering, concentrating, and freeze-drying to obtain the medicago sativa extract. The material-liquid ratio of the medicago sativa powder to the ethanol aqueous solution is 1:(5-9).

[0013] The second aspect of the present invention provides a method for preparing the barrier repair composition suitable for dry and sensitive skin according to the present invention, comprising the following steps: mixing each component to obtain the composition.

[0014] The third aspect of the present invention provides the application of the barrier repair composition suitable for dry and sensitive skin according to the present invention in the preparation of cosmetics.

[0015] The fourth aspect of the present invention provides a cosmetic, which contains the barrier repair composition suitable for dry and sensitive skin as described above.

[0016] According to some embodiments of the present invention, the cosmetic further comprises excipients acceptable in cosmetics.

[0017] According to some embodiments of the present invention, the excipients include at least one of an emulsifier, a polyol, a thickener, and a preservative.

[0018] According to some embodiments of the present invention, the addition amount of the composition in the cosmetic is 0.01-8 wt%.

[0019] According to some embodiments of the present invention, the cosmetic is one of an aqueous solution, a serum, an essence milk, a facial mask liquid, and a cream.

[0020] The extract of Camellia chrysantha contains rich active ingredients such as tea polyphenols, tea polysaccharides, flavonoids, saponins, proteins, amino acids, and various vitamins, which can repair the skin barrier, promote DNA damage repair, and have good effects in aspects such as antioxidant, anti-inflammatory and soothing, whitening, and moisturizing, and can be used to treat sensitive skin.

[0021] The extract of Eriobotrya japonica contains rich triterpenoid acids such as oleanolic acid and corosolic acid, which have an obvious sedative effect on the central nervous system, and play an analgesic and antipruritic effect by inhibiting the process of transmitting the itching sensation to the central nervous system through the stratum corneum and epidermal nerve fibers "stringing"; in addition, it also contains flavonoid compounds and saponin substances, which have an inhibitory effect on the activities of hyaluronidase and matrix metalloproteinase-1, can promote the proliferation of stratum corneum cells, have sedative, anti-inflammatory, antibacterial, and moisturizing effects, and can also accelerate the repair of damaged barriers, can solve the allergic problems caused by histamine, reduce the stinging and itching sensations, and reduce skin telangiectasia.

[0022] The extract of Medicago Sativa contains chemical components such as flavonoids, triterpenoids, alkaloids, coumarins, proteins and polysaccharides. It has antibacterial, antioxidant and moisturizing properties, can promote the generation of collagen, repair skin damage, has good inhibition on the activity of matrix metalloproteinase-1, can effectively repair skin sensitivity caused by inflammation, effectively relieve allergic reactions, and has excellent effects in repairing the skin barrier and improving skin moisture.

[0023] Hydrolyzed Royal Jelly Protein is a protein hydrolysate extracted from royal jelly, containing free amino acids such as proline and lysine, and mainly containing peptides. It can make the skin soft and smooth, stimulate human fibroblasts to regenerate collagen, reduce skin inflammation and erythema, prevent lactic acid-mediated allergy, and can be used in anti-aging, soothing and other products; it has good anti-irritation and collagen-promoting effects, can increase the content of skin collagen, relieve skin irritation, has strong antioxidant ability, can eliminate free radicals and prevent skin aging.

[0024] The beneficial effects of the present invention are:

[0025] The present invention provides a barrier repair composition suitable for dry and sensitive skin and its application. A skin barrier repair composition suitable for dry and sensitive skin is screened from a variety of plant raw materials with anti-inflammatory, soothing, moisturizing, skin barrier repair and other effects, and through reasonable collocation and synergistic effect, it can produce better skin barrier repair effect, thus fundamentally solving the problems of dryness, tightness, desquamation, erythema, itching, dullness, flushing, burning sensation, stinging sensation, etc. of dry and sensitive skin. The effect is much better than that of a single active ingredient, and is also superior to common anti-allergic and soothing ingredients such as Portulaca oleracea, Centella asiatica, Glycyrrhiza glabra and their combinations, and good synergistic effects are produced among the components. Specific embodiments

[0026] It should be understood that the present invention is not limited to the specific compositions, methods or schemes described herein. In addition, unless otherwise defined, all technical and scientific terms used herein have the same meanings as those commonly understood by those skilled in the art to which the present invention belongs. It should also be understood that the terms used herein are only for the purpose of describing specific embodiments and are not intended to limit the scope of the present invention, and the scope of the present invention is only defined by the claims.

[0027] In the description of the present invention, it should be noted that for those not specified in the examples, they are carried out according to conventional conditions or conditions recommended by the manufacturer. For reagents or instruments not specified by the manufacturer, they are all conventional products that can be obtained through commercial purchase.

[0028] In the following examples and comparative examples, the plant extracts in the barrier repair composition suitable for dry and sensitive skin were obtained by the following extraction methods respectively:

[0029] Preparation method of camellia nitidissima extract: Mix camellia nitidissima flower powder with an ethanol aqueous solution with a volume fraction of 60%, extract twice at 75°C, each extraction for 1.5 h, combine the extracts, filter, concentrate, and freeze-dry to obtain camellia nitidissima extract. The material-liquid ratio (weight-volume ratio) of the camellia nitidissima flower powder to the ethanol aqueous solution is 1:7.

[0030] Preparation method of eriobotrya japonica leaf extract: Mix eriobotrya japonica leaf powder with an ethanol aqueous solution with a volume fraction of 70%, extract twice at 75°C, each extraction for 1.5 h, combine the extracts, filter, concentrate, and freeze-dry to obtain eriobotrya japonica leaf extract. The material-liquid ratio (weight-volume ratio) of the eriobotrya japonica leaf powder to the ethanol aqueous solution is 1:6.

[0031] Preparation method of medicago sativa extract: Mix medicago sativa powder with an ethanol aqueous solution with a volume fraction of 80%, extract twice at 75°C, each extraction for 1 h, combine the extracts, filter, concentrate, and freeze-dry to obtain medicago sativa extract. The material-liquid ratio (weight-volume ratio) of the medicago sativa powder to the ethanol aqueous solution is 1:7.

[0032] Example 1

[0033] This example provides a barrier repair composition suitable for dry and sensitive skin. The composition includes the following components in parts by weight: 5 parts of camellia nitidissima extract, 16 parts of eriobotrya japonica leaf extract, 13 parts of medicago sativa extract, and 15 parts of hydrolyzed royal jelly protein.

[0034] The preparation method of the barrier repair composition suitable for dry and sensitive skin in this example is: Mix each component evenly.

[0035] Example 2

[0036] This example provides a barrier repair composition suitable for dry and sensitive skin. The composition includes the following components in parts by weight: 9 parts of camellia nitidissima extract, 14 parts of eriobotrya japonica leaf extract, 16 parts of medicago sativa extract, and 11 parts of hydrolyzed royal jelly protein.

[0037] The preparation method of the barrier repair composition suitable for dry and sensitive skin in this example is: Mix each component evenly.

[0038] Example 3

[0039] This example provides a barrier repair composition suitable for dry and sensitive skin. The composition includes the following components in parts by weight: 10 parts of camellia nitidissima extract, 12 parts of eriobotrya japonica leaf extract, 15 parts of medicago sativa extract, and 8 parts of hydrolyzed royal jelly protein.

[0040] The preparation method of the barrier repair composition applicable to dry and sensitive skin in this example is: mix all components evenly.

[0041] Example 4

[0042] This example provides a barrier repair composition applicable to dry and sensitive skin, and the composition includes the following components in parts by weight: 12 parts of camellia nitidissima extract, 10 parts of eriobotrya japonica leaf extract, 20 parts of medicago sativa extract, and 8 parts of hydrolyzed royal jelly protein.

[0043] The preparation method of the barrier repair composition applicable to dry and sensitive skin in this example is: mix all components evenly.

[0044] Comparative Example 1

[0045] Compared with Example 2, camellia nitidissima extract is not added in Comparative Example 1, and the other components and dosages are the same as those in Example 2.

[0046] Comparative Example 2

[0047] Compared with Example 2, eriobotrya japonica leaf extract is not added in Comparative Example 2, and the other components and dosages are the same as those in Example 2.

[0048] Comparative Example 3

[0049] Compared with Example 2, medicago sativa extract is not added in Comparative Example 3, and the other components and dosages are the same as those in Example 2.

[0050] Comparative Example 4

[0051] Compared with Example 2, hydrolyzed royal jelly protein is not added in Comparative Example 4, and the other components and dosages are the same as those in Example 2.

[0052] Comparative Example 5

[0053] Compared with Example 2, in Comparative Example 5, an equal amount of portulaca oleracea is used to replace camellia nitidissima extract, and the other components and dosages are the same as those in Example 2.

[0054] Comparative Example 6

[0055] Compared with Example 2, in Comparative Example 6, an equal amount of centella asiatica is used to replace eriobotrya japonica leaf extract, and the other components and dosages are the same as those in Example 2.

[0056] Comparative Example 7

[0057] Compared with Example 2, in Comparative Example 7, an equal amount of glycyrrhiza glabra is used to replace medicago sativa extract, and the other components and dosages are the same as those in Example 2.

[0058] Comparative Example 8

[0059] Compared with Example 2, in Comparative Example 8, the hydrolyzed royal jelly protein was replaced with an equal amount of acetyl heptapeptide-4, and the other components and dosages were the same as those in Example 2.

[0060] Application Example 1

[0061] This application example provides a serum containing the above-mentioned barrier repair composition suitable for dry and sensitive skin, and its formula is shown in Table 1.

[0062] Table 1

[0063]

[0064] The preparation method of this serum includes the following steps:

[0065] 1) Add PEG-40 hydrogenated castor oil, methylpolysiloxane, and sunflower seed oil to the oil phase pot, and stir at 80 °C until completely dissolved to obtain the oil phase;

[0066] 2) Add glycerol, propylene glycol, sodium hyaluronate, and deionized water to the water phase pot, and stir at 80 °C until completely dissolved to obtain the water phase;

[0067] 3) Slowly suck the water phase in step 2) into the oil phase, and homogenize and emulsify to obtain a mixture;

[0068] 4) Cool the mixture to 50 °C, add the above-mentioned barrier repair composition suitable for dry and sensitive skin and p-hydroxyacetophenone, stir and cool to room temperature, and discharge to obtain the serum.

[0069] Application Examples 2-4

[0070] Compared with Application Example 1, in Application Examples 2-4, the barrier repair composition suitable for dry and sensitive skin provided in Example 1 was replaced with the barrier repair compositions suitable for dry and sensitive skin provided in Examples 2-4 respectively, and the other components, dosages and preparation methods were the same as those in Application Example 1.

[0071] Comparative Application Examples 1-8

[0072] Compared with Application Example 1, in Comparative Application Examples 1-8, the barrier repair composition suitable for dry and sensitive skin provided in Example 1 was replaced with the barrier repair compositions suitable for dry and sensitive skin provided in Comparative Examples 1-8 respectively, and the other components, dosages and preparation methods were the same as those in Application Example 1.

[0073] Efficacy Test:

[0074] I. Human Patch Test

[0075] The human patch test was used to verify the irritation of Application Examples 1-4 of this invention to dry and sensitive skin. A total of 30 subjects were selected for the screening and verification test, with half male and half female, the youngest age: 20 years old, the oldest age: 50 years old, meeting the volunteer selection criteria for subjects.

[0076] First, a suitable patch tester was selected. Using the closed patch test method, about 0.020 - 0.025 ml of the test substance was placed in the patch tester, and the medical tape was applied externally to the back of the subject. After 24 hours, the test substance was removed, and the skin reactions were observed at 0.5, 24, and 48 hours after removing the patch tester. The observation results were recorded according to the skin reaction grading standard in the "Technical Specifications for Cosmetics Safety" (2015 Edition). The grading standard for skin adverse reactions is shown in Table 2:

[0077] Table 2 Grading Standard for Skin Reactions in the Closed Patch Test of Skin

[0078]

[0079] Finally, the results of the human skin patch test are shown in Table 3:

[0080] Table 3 Results of the Human Skin Patch Test

[0081]

[0082]

[0083] The results showed that among the 30 subjects, no skin adverse reactions occurred in Application Examples 1-4, indicating that the essence containing the above-mentioned barrier repair composition suitable for dry and sensitive skin in Application Examples 1-4 of the present invention is safe and non-irritating. Second, the content of inflammatory factor IL-6 was detected in macrophages

[0084] 1 Test Purpose and Principle

[0085] Macrophages can be used as a cell model to study the inhibition of the content of inflammatory factor IL-6 in cosmetics. By stimulating with lipopolysaccharide (LPS) of bacteria, the content of inflammatory factor IL-6 in the negative control and the test sample group was measured to evaluate whether the test sample has efficacy in inhibiting the content of inflammatory factor IL-6. The determination of the content of IL-6 was carried out by the enzyme-linked immunosorbent assay (ELISA).

[0086] In this test, referring to the laboratory method, the determination results of the content of cell IL-6 inflammatory factor of the test sample and the negative control were compared. If the content of cell IL-6 inflammatory factor of the test sample is lower than that of the negative control and has a significant difference (P < 0.05), it can be considered that the test sample has a certain soothing effect.

[0087] 2 Test Materials and Methods

[0088] 2.1 Instrumentation: RT-6100 microplate reader.

[0089] 2.2 Macrophages used in the experiment: RAW264.7 cells.

[0090] 2.3 Test methods

[0091] (1) Treatment of test samples

[0092] Sample group: Dilute the essence obtained in Application Examples 1-4 and Comparative Application Examples 1-8 with pure water to a solution with a concentration of 0.5%.

[0093] Negative control: Basic culture medium containing LPS.

[0094] (2) Test operation steps

[0095] Determination of IL-6 inflammatory factor content: Seed RAW264.7 cells in a 96-well plate. After culturing for 24 h, replace the medium with basic culture medium containing test samples at different concentrations. After 24 h, collect the cell supernatant and centrifuge. Pipette the supernatant and use an ELISA kit to measure the IL-6 content in the cell culture supernatant to obtain the effect of the test samples on the IL-6 content of macrophages. Among them, the cell culture medium without LPS is used as the blank control (BC), and the positive control substance diluted with the cell culture medium containing LPS is used as the positive control (PC, sodium dexamethasone phosphate).

[0096] (3) Calculation formula

[0097]

[0098] (4) Data analysis

[0099] The statistical analysis software is SPSS. The independent samples t-test is used for comparison between the test samples and the negative control. The above statistical analyses are all two-tailed tests, and the significance level is α = 0.05. P > 0.05 indicates no significant difference between the two groups; P < 0.05 indicates a significant difference between the two groups.

[0100] The test results of the IL-6 inflammatory factor content are shown in Table 4:

[0101] Table 4 Test results of IL-6 inflammatory factor content

[0102]

[0103] Note: If P > 0.05, it indicates no significant difference, denoted as "ns"; if P < 0.05, it indicates a significant difference, and when 0.01 < P ≤ 0.05, it is denoted as "*"; when 0.001 < P ≤ 0.01, it is denoted as "**"; when 0.0001 < P ≤ 0.001, it is denoted as "***"; when P ≤ 0.0001, it is denoted as "****"

[0104] Result analysis: BC is the experimental blank control, NC is the experimental negative control, and the PC group is the positive control. Among them, the IL-6 content in the NC group is much higher than that in the blank control group, and there is a significant difference between the two (P < 0.05), indicating that the experiment is valid.

[0105] As can be seen from Application Examples 1-4, when the barrier repair composition suitable for dry and sensitive skin contains camellia extract, loquat leaf extract, alfalfa extract, and hydrolyzed royal jelly protein at the same time, it has a good inhibitory effect on the inflammatory factor IL-6. And when the weight ratio of camellia extract, loquat leaf extract, alfalfa extract, and hydrolyzed royal jelly protein is 9:14:16:11, the inhibitory effect on the inflammatory factor IL-6 is the best.

[0106] As can be seen from Application Example 2 and Comparative Application Examples 1-4, when any one of camellia extract, loquat leaf extract, alfalfa extract, and hydrolyzed royal jelly protein is missing from the barrier repair composition suitable for dry and sensitive skin, its inhibitory effect on the inflammatory factor IL-6 will be significantly worse. From this, it can be seen that there is a certain synergistic effect among camellia extract, loquat leaf extract, alfalfa extract, and hydrolyzed royal jelly protein.

[0107] As can be seen from Application Example 2 and Comparative Application Examples 5-8, even when a certain component in the barrier repair composition suitable for dry and sensitive skin is replaced with common anti-allergic and soothing ingredients such as purslane, centella asiatica, licorice, and acetyl heptapeptide-4, its inhibitory effect on the inflammatory factor IL-6 is not as good as that of the application example. Therefore, not any combination of active ingredients can produce a synergistic effect, and camellia extract, loquat leaf extract, alfalfa extract, and hydrolyzed royal jelly protein cannot be replaced with each other.

[0108] III. Hyaluronidase inhibition rate test

[0109] 1 Test purpose and principle

[0110] Hyaluronidase is a specific lyase of hyaluronic acid and is a participant in allergic reactions, which has a strong correlation with the release of histamine by mast cells. Whether the test sample has a soothing effect can be judged by the hyaluronidase inhibition rate. The higher the hyaluronidase inhibition rate, the stronger the soothing effect of the substance, and vice versa.

[0111] In this experiment, the hyaluronidase inhibition rate test results of the test sample were compared with those of the negative control. If the inhibition rate of the test sample was higher than that of the negative control and there was a significant difference (P<0.05), it could be considered that the test sample had a certain soothing effect.

[0112] 2 Test Materials and Methods

[0113] 2.1 Instrument and Equipment: BSA224S Analytical Balance, L6s UV-Visible Spectrophotometer.

[0114] 2.2 Reagents: BR-grade hyaluronidase, BR-grade sodium hyaluronate.

[0115] 2.3 Test Method

[0116] (1) Treatment of Control and Test Samples

[0117] Sample Group: Dilute the compositions prepared in Application Examples 1-4 and Comparative Application Examples 1-8 with pure water to a composition concentration of 1%;

[0118] Positive Control (Dipotassium Glycyrrhizinate, purity ≥98%): Dilute with water to a positive control concentration of 2%;

[0119] Negative Control: Pure water.

[0120] (2) Test Operation Steps

[0121] Set up a sample group, a sample background group, a solvent group, and a solvent background group. Three parallels need to be set up for each group. Add different reagent solutions to the four groups respectively, shake well, let stand at room temperature for 30 min for color development, and measure the absorbance value at a wavelength of 528 nm with a UV-visible spectrophotometer.

[0122] (3) Calculation Formula

[0123]

[0124] Where:

[0125] A—Absorbance of the reaction solution without the sample;

[0126] B—Absorbance of the reaction solution without the sample and enzyme;

[0127] C—Absorbance of the reaction solution containing the sample and enzyme;

[0128] D—Absorbance of the reaction solution containing the sample and without enzyme.

[0129] (4) Data Analysis

[0130] The statistical analysis software was SPSS. The independent samples t-test was used to compare the hyaluronidase inhibition rates among the test samples, positive control substances, and negative control substances. All the above statistical analyses were two-tailed tests, and the significance level was a = 0.05. P ≥ 0.05 indicates no significant difference between the two groups; P < 0.05 indicates a significant difference between the two groups.

[0131] The test results of the hyaluronidase inhibition rate are shown in Table 5:

[0132] Table 5 Test Results of Hyaluronidase Inhibition Rate

[0133]

[0134]

[0135] Result analysis:

[0136] As can be seen from Application Examples 1-4, when the barrier repair composition suitable for dry and sensitive skin contains camellia extract, loquat leaf extract, alfalfa extract, and hydrolyzed royal jelly protein at the same time, it has good inhibitory effects on hyaluronidase. And when the weight ratio of camellia extract, loquat leaf extract, alfalfa extract, and hydrolyzed royal jelly protein is 9:14:16:11, the inhibitory effect on hyaluronidase is the best.

[0137] As can be seen from Application Example 2 and Comparative Application Examples 1-4, when any one of camellia extract, loquat leaf extract, alfalfa extract, and hydrolyzed royal jelly protein is missing from the barrier repair composition suitable for dry and sensitive skin, its inhibitory effect on hyaluronidase will be significantly worse. From this, it can be known that there is a certain synergistic effect among camellia extract, loquat leaf extract, alfalfa extract, and hydrolyzed royal jelly protein.

[0138] As can be seen from Application Example 2 and Comparative Application Examples 5-8, even when a certain component in the barrier repair composition suitable for dry and sensitive skin is replaced with common anti-allergic and soothing ingredients such as purslane, centella asiatica, licorice, and acetyl heptapeptide-4, its inhibitory effect on hyaluronidase is not as good as that of the application example. Therefore, not any combination of active ingredients can produce a synergistic effect, and camellia extract, loquat leaf extract, alfalfa extract, and hydrolyzed royal jelly protein cannot be replaced with each other.

[0139] IV. Transdermal Water Loss Rate Test of Skin

[0140] Test instrument: Skin water loss measuring instrument Aquaflux;

[0141] Test stages: 10 days (D10), 28 days (D28);

[0142] Test subjects: A total of 120 subjects with dry and sensitive skin in good health were selected, with 60 males and 60 females. The faces of the subjects had mild inflammation or allergies, and all had symptoms such as dryness and flushing. They were evenly divided into... The subjects were randomly divided into 12 groups, with 10 people in each group. The subjects applied the corresponding essence samples evenly on their faces every morning and evening.

[0143] Measurement area: Cheek (left / right);

[0144] Explanation of test parameters: Trans-epidermal water loss: The lower the value, the less trans-epidermal water loss. Unit: g / (h·m 2 ). Trans-epidermal water loss rate (%) = (Dn - D0) × 100% / D0, where Dn can be the trans-epidermal water loss of the subject's skin at D10 or D28, and D0 is the initial trans-epidermal water loss of the subject's skin. The test results are shown in Table 6.

[0145] Table 6 Test results of skin trans-epidermal water loss rate

[0146]

[0147]

[0148] Result analysis:

[0149] From Application Examples 1 - 4, it can be seen that when the barrier repair composition suitable for dry and sensitive skin contains camellia extract, loquat leaf extract, alfalfa extract, and hydrolyzed royal jelly protein at the same time, the corresponding trans-epidermal water loss rate of the skin decreases significantly, and the barrier function and water retention ability of the skin are significantly improved. Moreover, when the weight ratio of camellia extract, loquat leaf extract, alfalfa extract, and hydrolyzed royal jelly protein is 9:14:16:11, the skin barrier repair effect is the best.

[0150] From Application Example 2 and Comparative Application Examples 1 - 4, it can be seen that when the barrier repair composition suitable for dry and sensitive skin lacks any one of camellia extract, loquat leaf extract, alfalfa extract, and hydrolyzed royal jelly protein, the corresponding skin water loss rate increases, and the skin barrier repair effect becomes worse. From this, it can be known that there is a certain synergistic effect among camellia extract, loquat leaf extract, alfalfa extract, and hydrolyzed royal jelly protein.

[0151] From Application Example 2 and Comparative Application Examples 5 - 8, it can be seen that even when a certain component in the barrier repair composition suitable for dry and sensitive skin is replaced with common anti-allergic and soothing ingredients such as purslane, centella asiatica, licorice, and acetyl heptapeptide-4, the corresponding skin water loss rate increases. Therefore, not any combination of active ingredients can produce a synergistic effect, and camellia extract, loquat leaf extract, alfalfa extract, and hydrolyzed royal jelly protein cannot be replaced with each other.

[0152] V. Human Efficacy Experiment

[0153] The following will verify through experiments the soothing, redness-reducing, moisturizing, and desquamation-improving effects of the essence liquids containing the above-mentioned barrier repair compositions suitable for dry and sensitive skin in Application Examples 1-4 and Comparative Application Examples 1-8 on dry and sensitive skin.

[0154] A total of 120 subjects with dry and sensitive skin in good health were selected, with half male and half female. The faces of the subjects had mild inflammation or allergies, and there were symptoms such as dryness and flushing on the faces. They were evenly divided into... The subjects were randomly divided into 12 groups, with 10 people in each group. The subjects applied the corresponding essence liquid samples of each group evenly on their faces every morning and evening, and the trial effects were followed up in the form of a questionnaire after 1 month. The samples were scored for their effects, with the scoring range being 1-10 points, and the higher the score, the better the effect. 10 points indicated obvious effects, and the average was taken. The evaluation criteria for obvious effects were obtained from the comparison of the images before and after use. The specific results are shown in Table 7.

[0155] Table 7 Results of Human Efficacy Experiment

[0156] Group Soothing effect Redness reduction effect Moisturizing effect Improvement effect on desquamation Application Example 1 9.1 8.5 8.9 8.5 Application Example 2 9.2 8.7 9.1 9.1 Application Example 3 8.4 8.8 8.4 8.9 Application Example 4 8.9 8.3 8.5 8.7 Comparative Application Example 1 6.8 6.2 7.1 6.7 Comparative Application Example 2 6.2 6.5 6.5 6.3 Comparative Application Example 3 6.8 6.6 5.4 5.9 Comparative Application Example 4 6.9 5.8 6.2 6.7 Comparative Application Example 5 6.1 5.8 6.3 5.7 Comparative Application Example 6 6.3 6.7 6.3 7.1 Comparative Application Example 7 6.2 5.8 6.8 6.6 Comparative Application Example 8 5.6 6.4 6.1 6.3

[0157] It can be clearly seen from Table 7 that the essence liquids of Application Examples 1-4 of the present application have obvious soothing, redness-reducing, moisturizing, and desquamation-improving effects on dry and sensitive skin, and the effects are significantly better than those of Comparative Application Examples 1-8. It can be seen that there is an obvious synergistic effect among camellia flower extract, loquat leaf extract, alfalfa extract, and hydrolyzed royal jelly protein.

[0158] The above embodiments are preferred embodiments of the present invention, but the embodiments of the present invention are not limited by the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications made without departing from the spirit and principle of the present invention shall be equivalent replacement methods and are all included in the protection scope of the present invention.

Claims

1. A barrier repair composition suitable for dry and sensitive skin, characterized in that, The composition comprises camellia nitidissima extract, eriobotrya japonica leaf extract, medicago sativa extract, and hydrolyzed royal jelly protein.

2. The barrier repair composition suitable for dry and sensitive skin according to claim 1, wherein The weight ratio of the camellia nitidissima extract, the eriobotrya japonica leaf extract, the medicago sativa extract, and the hydrolyzed royal jelly protein is (5-12):(10-16):(13-20):(8-15).

3. The barrier repair composition suitable for dry and sensitive skin according to claim 1, characterized in that, The weight ratio of the camellia nitidissima extract, the eriobotrya japonica leaf extract, the medicago sativa extract, and the hydrolyzed royal jelly protein is 9:14:16:

11.

4. The barrier repair composition suitable for dry and sensitive skin according to any one of claims 1-3, characterized in that, The preparation method of the camellia nitidissima extract comprises the following steps: Mix camellia nitidissima flower powder with an ethanol aqueous solution with a volume fraction of 50-70%, extract 2-3 times at 70-80°C, each extraction for 1-2 h, combine the extraction solutions, filter, concentrate, and freeze-dry to obtain the camellia nitidissima extract. The material-liquid ratio of the camellia nitidissima flower powder to the ethanol aqueous solution is 1:(6-8); The preparation method of the eriobotrya japonica leaf extract comprises the following steps: Mix eriobotrya japonica leaf powder with an ethanol aqueous solution with a volume fraction of 60-80%, extract 2-3 times at 70-80°C, each extraction for 1-2 h, combine the extraction solutions, filter, concentrate, and freeze-dry to obtain the eriobotrya japonica leaf extract. The material-liquid ratio of the eriobotrya japonica leaf powder to the ethanol aqueous solution is 1:(5-9); The preparation method of the medicago sativa extract comprises the following steps: Mix medicago sativa powder with an ethanol aqueous solution with a volume fraction of 70-90%, extract 2-3 times at 70-80°C, each extraction for 0.5-1 h, combine the extraction solutions, filter, concentrate, and freeze-dry to obtain the medicago sativa extract. The material-liquid ratio of the medicago sativa powder to the ethanol aqueous solution is 1:(5-9).

5. The preparation method of the barrier repair composition applicable to dry and sensitive skin according to any one of claims 1-4, characterized in that, It comprises the following steps: Mix the components to obtain the composition.

6. Use of the barrier repair composition suitable for dry and sensitive skin according to any one of claims 1-4 in the preparation of cosmetics.

7. A cosmetic, characterized in that, Containing the barrier repair composition suitable for dry and sensitive skin according to any one of claims 1-4.

8. The cosmetic according to claim 7, characterized in that, The cosmetic further comprises excipients acceptable in the cosmetic, and the excipients include at least one of an emulsifier, a polyol, a thickener, and a preservative.

9. The cosmetic according to claim 7, characterized in that, The addition amount of the barrier repair composition suitable for dry and sensitive skin in the cosmetic is 0.01-8 wt%.

10. The cosmetic according to any one of claims 7-9, characterized in that, The cosmetic is one of an aqueous solution, a serum, a lotion, a mask liquid, or a cream.

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