Mixed fermentation process of antibacterial composite lipopeptide

Abstract

The invention discloses an antibacterial composite lipopeptide mixed fermentation process, and belongs to the technical field of microbial cultivation, fermentation equipment adopted by the fermentation process comprises a fermentation tank and a motor, a rotating shaft is rotatably connected to the interior of the fermentation tank, a first gear is arranged at the bottom of the rotating shaft, the output end of the motor is connected with a second gear, and the second gear is meshed with the first gear; scraping strips are arranged on the side wall of the rotating shaft, a prism shaft is sleeved with the rotating shaft, mixing blades are arranged on the side wall of the prism shaft, and a transmission shaft is arranged on the surfaces of the mixing blades. When the mixing blades rotate around the axis of the rotating shaft for stirring, the mixing blades can do reciprocating motion in the vertical direction along the axis of the rotating shaft, so that the action stirring range of the mixing blades can be widened, dead corners and blind areas are avoided, the turbulence generation frequency in the lifting rotating process is increased, and the exchange and mixing effects of a culture solution are improved; a boundary layer generated by accumulation of fermentation metabolites of a single flora under the action of centrifugal force or gravity in the fermentation process of multiple florae can be broken.

Description

Technical Field

[0001] The invention relates to the technical field of microbial cultivation, and in particular to an antibacterial composite lipopeptide mixed fermentation process. Background Art

[0002] Antimicrobial lipopeptides are generally metabolites of bacteria such as Bacillus subtilis or Bacillus licheniformis. They have a broad spectrum of bactericidal properties, no toxic side effects, no immunogenicity, and do not produce drug resistance. They have a strong killing effect on harmful bacteria, viruses, pathogens and mycoplasmas, and are an ideal alternative to antibiotics.

[0003] When the existing antimicrobial composite lipopeptide production technology adopts a mixed fermentation process, by mixing and culturing Bacillus subtilis, Bacillus licheniformis and other bacterial communities for fermentation, the metabolites of the two can promote each other's growth, further promote the production of some metabolites, and improve the production efficiency of antimicrobial lipopeptides. When the bacterial community fermentation device of the prior art performs mixed fermentation, rotary stirring is generally used to mix and ferment the bacterial community, but this mixing method is prone to dead corners, and the degree of mixing of the culture solution is low, so that the metabolic products of the bacterial community and the bacterial community in the mixed fermentation process are easy to form aggregated colonies due to the density difference under the action of centrifugal force, affecting the overall mixed fermentation efficiency.

[0004] How to invent an antibacterial composite lipopeptide mixed fermentation process to improve these problems has become an urgent problem to be solved by technicians in this field. Summary of the invention

[0005] In order to make up for the above shortcomings, the present invention provides an antibacterial composite lipopeptide mixed fermentation process, aiming to improve the problems raised by the above background technology.

[0006] The present invention is achieved in that: The invention provides an antibacterial composite lipopeptide mixed fermentation process. The fermentation equipment used in the fermentation process includes a fermentation tank and a motor. The fermentation tank is rotatably connected with a rotating shaft, a gear one is arranged at the bottom of the rotating shaft, a gear two is connected to the output end of the motor, the gear two is meshed with the gear one, a scraper is arranged on the side wall of the rotating shaft, a prismatic shaft is sleeved inside the rotating shaft, a mixing blade is arranged on the side wall of the prismatic shaft, a transmission shaft is arranged on the surface of the mixing blade, and uniformly distributed thinning blades are arranged on the surface of the transmission shaft. The fermentation tank is also provided with: A driving mechanism, used for driving the mixing blades to reciprocate in a vertical direction while rotating; A supply mechanism, which supplies liquid and oxygen to the inside of the fermentation tank during the fermentation process; A regulating mechanism that adjusts the supply of nutrients and oxygen according to the degree of fermentation; The driving mechanism includes a support frame arranged inside the fermentation tank, a sleeve is fixedly installed on the top of the support frame, an optical axis is arranged at one end of the prismatic shaft extending into the sleeve, a reciprocating thread is arranged on the outer wall of the optical axis, and a ball matched with the reciprocating thread is arranged on the inner wall of the sleeve; The fermentation process includes the following steps: S1: Inoculate activated single colonies of Bacillus subtilis, Bacillus berberis, and Bacillus amyloliquefaciens into liquid culture medium at a ratio of 1:1:1; S2: Mix the liquid culture medium and put it into the fermentation tank; S3: The fermenter is set to a constant temperature and supplied with sterile air; S4: The motor starts to drive the liquid inside the fermentation tank to mix and ferment through the mixing blades; S5: After fermentation, the fermentation liquid is discharged from the fermentation tank, and the crude composite antimicrobial lipopeptide is obtained through operations such as filtration and centrifugation.

[0007] Preferably, the supply mechanism includes a pump liquid chamber opened inside the rotating shaft, a feeding barrel is provided at the bottom of the fermentation tank, a nutrient solution is provided inside the feeding barrel, one end of the prismatic shaft extending into the pump liquid chamber is connected to a piston through a spring, a prismatic shaft is provided inside the prismatic shaft, a sleeve is connected to the prismatic shaft, the sleeve passes through the piston and is connected to the pump liquid chamber, a suction tube extending to the inside of the feeding barrel is provided at the bottom of the pump liquid chamber, a connecting tube connected to the feeding tube is provided inside the mixing blade, a movable blade is movably connected to the mixing blade, a spray hole cooperating with the refinement blade is provided on the surface of the movable blade, and the spray hole is connected to the connecting tube.

[0008] Preferably, a pump air cavity is opened inside the rotating shaft, a piston 2 is connected to the side wall of the prismatic shaft through a spring sleeve, and the piston 2 is movably sleeved with the pump air cavity. An air supply pipe connected to the pump air cavity is opened inside the prismatic shaft, and a one-way valve for air intake is provided on the side wall of the pump air cavity. Two groups of movable blades and corresponding connecting tubes are provided, the air supply pipe is connected to a group of adjacent connecting tubes, and the liquid supply pipe is connected to a group of adjacent connecting tubes.

[0009] Preferably, a gear three is arranged at one end of the transmission shaft close to the rotating shaft, and a rack matching with the sleeve is arranged inside the rotating shaft.

[0010] Preferably, the regulating mechanism includes a sealing groove opened inside the mixing blade, the side wall of the movable blade is provided with a rectangular frame matching the sealing groove, a spring is provided between the mixing blade and the movable blade, the side wall of the movable blade is provided with a supply pipe connected to the spray hole, the side wall of the movable blade is provided with an regulating chamber, one end of the supply pipe located inside the mixing blade extends to the inside of the regulating chamber, the inside of the regulating chamber is sleeved with a valve block, the valve block is sleeved with the supply pipe, the valve block is provided with an output pipe connected to the supply pipe, and the output pipe is movably sleeved with the movable pipe.

[0011] Preferably, the movable tube is of L-shaped design, one end of the movable tube is movably connected to the output tube, and the other end of the movable tube is movably connected to the connecting tube.

[0012] Preferably, a valve groove is provided on the side wall of the supply pipe, and a circle of connecting grooves cooperating with the output pipe and the valve groove is provided inside the valve block.

[0013] Preferably, the valve slot is designed to be an acute-angle triangle, and the acute-angle side of the valve slot is designed to face the side away from the hydraulic pipe.

[0014] Preferably, a hydraulic pipe is provided at one end of the regulating chamber away from the supply pipe, one end of the hydraulic pipe is communicated with the regulating chamber, and the other end of the hydraulic pipe is communicated with the inside of the sealing groove.

[0015] In summary, the beneficial effects of the present invention are: 1. When the mixing blades rotate around the axis of the shaft for stirring, they can also reciprocate in the vertical direction along the axis of the shaft, which can increase the stirring range of the mixing blades, avoid dead corners and blind spots, increase the frequency of turbulence generated during rotation, and improve the exchange and mixing effect of the culture solution. It is helpful to break the boundary layer produced by the accumulation of fermentation metabolites of a single bacterial community under the action of centrifugal force or gravity during the fermentation of multiple bacterial communities. At the same time, during the stirring process, the reciprocating movement of the prismatic axis can automatically add nutrients and gas supply to the culture solution, and the rotation of the refinement blades can make the nutrients and oxygen quickly and evenly distributed in the culture solution, so as to realize automatic replenishment of nutrients and oxygen during the fermentation process, maintain the concentration of the culture solution and nutrient requirements, and improve the production efficiency of mixed fermentation.

[0016] 2. In the early stage of fermentation, sufficient nutrients and oxygen can be supplied to the culture medium in the fermentation tank. As the fermentation proceeds, the metabolites increase. By detecting the fluidity and viscosity changes of the solution and amplifying the detection results, the supply of nutrients and gas to the nozzle is adjusted and reduced, and the supply of nutrients and gas to the culture medium is automatically adjusted according to the fermentation process. While reducing waste and improving resource utilization, it meets the needs of bacteria for nutrients and gas at different stages, and adjusts itself to adapt to the growth and metabolism of various bacteria, effectively improving the economic benefits of mixed fermentation production. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for use in the embodiments will be briefly introduced below. It should be understood that the following drawings only show certain embodiments of the present invention and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other related drawings can be obtained based on these drawings without paying creative work.

[0018] Figure 1 It is a schematic diagram of the exterior of a fermentation tank provided in an embodiment of the present invention.

[0019] Figure 2 It is a schematic diagram of the interior of a fermentation tank provided in an embodiment of the present invention.

[0020] Figure 3 It is an overall schematic diagram of the rotating shaft provided in an embodiment of the present invention.

[0021] Figure 4 It is a schematic diagram of the interior of the sleeve provided in an embodiment of the present invention.

[0022] Figure 5 It is a schematic diagram of the interior of the pump liquid chamber provided in an embodiment of the present invention.

[0023] Figure 6 It is a schematic diagram of the interior of the pump air cavity provided in an embodiment of the present invention.

[0024] Figure 7 It is an overall schematic diagram of a mixing blade provided in an embodiment of the present invention.

[0025] Figure 8 It is an overall schematic diagram of the movable blade provided in an embodiment of the present invention.

[0026] Fig. 9 It is an overall schematic diagram of the adjustment cavity provided in an embodiment of the present invention.

[0027] Fig.10 It is a schematic diagram of the interior of the adjustment cavity provided in an embodiment of the present invention.

[0028] Legend: 100, fermentation tank; 101, feed barrel; 102, motor; 103, gear 1; 104, gear 2; 105, support frame; 200, rotating shaft; 201, prismatic shaft; 202, scraper; 203, pump liquid chamber; 204, pump air chamber; 205, sleeve; 206, pipette; 207, piston 1; 208, sleeve; 209, liquid supply pipe; 210, piston 2; 211, air supply pipe; 212, optical axis; 21 3. reciprocating thread; 214. rack; 300. mixing blade; 301. movable blade; 302. sealing groove; 303. connecting pipe; 304. transmission shaft; 305. gear three; 306. rectangular frame; 307. supply pipe; 308. regulating chamber; 309. movable pipe; 310. spray hole; 311. output pipe; 312. hydraulic pipe; 313. valve slot; 314. connecting slot; 315. refinement blade; 316. valve block. DETAILED DESCRIPTION

[0029] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0030] Reference Figure 1-10 The present invention provides an antibacterial composite lipopeptide mixed fermentation process. The fermentation equipment used in the fermentation process includes a fermentation tank 100 and a motor 102. The fermentation tank 100 is rotatably connected with a rotating shaft 200. The bottom of the rotating shaft 200 is provided with a gear 103. The output end of the motor 102 is connected with a gear 2 104. The gear 2 104 is meshed with the gear 1 103. The side wall of the rotating shaft 200 is provided with a scraper 202. The interior of the rotating shaft 200 is sleeved with a prismatic shaft 201. The side wall of the prismatic shaft 201 is provided with a mixing blade 300. The surface of the mixing blade 300 is provided with a transmission shaft 304. The surface of the transmission shaft 304 is provided with uniformly distributed thinning blades 315. The interior of the fermentation tank 100 is also provided with: A driving mechanism, used to drive the mixing blade 300 to reciprocate in a vertical direction while rotating; A supply mechanism for supplying liquid and oxygen to the interior of the fermentation tank 100 during the fermentation process; A regulating mechanism that adjusts the supply of nutrients and oxygen according to the degree of fermentation; The driving mechanism includes a support frame 105 disposed inside the fermentation tank 100, a sleeve 205 is fixedly mounted on the top of the support frame 105, an optical axis 212 is disposed at one end of the prismatic shaft 201 extending into the inside of the sleeve 205, a reciprocating thread 213 is provided on the outer side wall of the optical axis 212, and a ball matched with the reciprocating thread 213 is provided on the inner side wall of the sleeve 205; The fermentation process includes the following steps: S1: Inoculate activated single colonies of Bacillus subtilis, Bacillus berberis, and Bacillus amyloliquefaciens into liquid culture medium at a ratio of 1:1:1; S2: Mixing the liquid culture medium and putting it into the fermentation tank 100; S3: The fermentation tank 100 is set to a constant temperature and sterile air is supplied; S4: The motor 102 starts to drive the liquid inside the fermentation tank 100 to mix and ferment through the mixing blades 300; S5: After the fermentation is completed, the fermentation liquid is discharged from the fermentation tank 100, and a crude composite antimicrobial lipopeptide is obtained by filtering and centrifuging.

[0031] It should be noted that when the bacterial community of Bacillus subtilis, Bacillus berberis, and Bacillus amyloliquefaciens was mixed for fermentation, the inoculation amount was 3%, the ratio was 1:1:1, and the lipopeptide composition of the product was close to 3:5:2.

[0032] Reference Figure 3-7 The supply mechanism includes a pump liquid chamber 203 opened inside the rotating shaft 200, a feed barrel 101 is arranged at the bottom of the fermentation tank 100, and a nutrient solution is arranged inside the feed barrel 101. The nutrient solution includes nutrients such as carbon sources, phosphates, trace elements, etc. according to different fermentation requirements. One end of the prism shaft 201 extending into the pump liquid chamber 203 is connected to a piston 207 through a spring, and a prism shaft 201 is opened inside the prism shaft 201, and the prism shaft 201 is sleeved with a sleeve 208, the sleeve 208 passes through the piston 207 and is connected with the pump liquid chamber 203, the bottom of the pump liquid chamber 203 is provided with a suction tube 206 extending to the inside of the supply barrel 101, the interior of the mixing blade 300 is provided with a connecting tube 303 connected with the supply tube 209, the interior of the mixing blade 300 is movably sleeved with a movable blade 301, and the surface of the movable blade 301 is provided with a spray hole 310 that cooperates with the refinement blade 315, and the spray hole 310 is connected with the connecting tube 303.

[0033] Furthermore, a pump air cavity 204 is provided inside the rotating shaft 200, a piston 210 is connected to the side wall of the prismatic shaft 201 through a spring sleeve, and the piston 210 is movably sleeved with the pump air cavity 204. An air supply pipe 211 connected to the pump air cavity 204 is provided inside the prismatic shaft 201, and a one-way valve for air intake is provided on the side wall of the pump air cavity 204. A one-way valve for air intake is also provided inside the air supply pipe 211. Two groups of movable blades 301 and corresponding connecting pipes 303 are provided. The air supply pipe 211 is connected to a group of connecting pipes 303 that are adjacent to each other, and the liquid supply pipe 209 is connected to a group of connecting pipes 303 that are adjacent to each other.

[0034] It should be noted that, through the spring design of piston two 210 and piston one 207, when the flow area of ​​the valve slot 313 becomes smaller, a buffer zone can be provided by the spring.

[0035] It should be noted that a gear 305 is disposed at one end of the transmission shaft 304 close to the rotating shaft 200 , and a rack 214 cooperating with the sleeve 205 is disposed inside the rotating shaft 200 .

[0036] Reference Figure 3-10The regulating mechanism includes a sealing groove 302 opened inside the mixing blade 300, a rectangular frame 306 matching the sealing groove 302 is provided on the side wall of the movable blade 301, the sealing groove 302 and the rectangular frame 306 are movably connected, and hydraulic oil is provided between the sealing groove 302 and the rectangular frame 306, a spring is provided between the mixing blade 300 and the movable blade 301, a supply pipe 307 connected to the spray hole 310 is provided on the side wall of the movable blade 301, and an regulating chamber 308 is provided on the side wall of the movable blade 301. One end of the supply pipe 307 located inside the mixing blade 300 extends to the inside of the regulating chamber 308, and a valve block 316 is connected inside the regulating chamber 308. The valve block 316 is connected to the supply pipe 307, and the valve block 316 is provided with an output pipe 311 connected to the supply pipe 307, and the output pipe 311 is movably connected to the movable pipe 309.

[0037] Furthermore, the movable tube 309 is L-shaped, one end of the movable tube 309 is movably connected to the output tube 311, and the other end of the movable tube 309 is movably connected to the connecting tube 303, so that when the movable blade 301 and the output tube 311 move, they can maintain a connected state through the movable tube 309.

[0038] Furthermore, a valve groove 313 is formed on the side wall of the supply pipe 307 , and a circle of connecting grooves 314 cooperating with the output pipe 311 and the valve groove 313 is formed inside the valve block 316 .

[0039] Furthermore, the valve groove 313 is designed as an acute triangle, and the acute angle side of the valve groove 313 is designed to be away from the hydraulic pipe 312. It should be noted that when the connecting groove 314 is connected with different areas of the valve groove 313 during movement, different flow cross-sectional areas of the connecting areas can be achieved.

[0040] It should be noted that a hydraulic tube 312 is provided at one end of the regulating chamber 308 away from the supply tube 307 , one end of the hydraulic tube 312 is connected to the regulating chamber 308 , and the other end of the hydraulic tube 312 is connected to the inside of the sealing groove 302 through a hose passing through the rectangular frame 306 .

[0041] The working process of the antibacterial composite lipopeptide mixed fermentation process is as follows: The mixed culture liquid of Bacillus subtilis, Bacillus berezius and Bacillus amyloliquefaciens is put into the fermentation tank 100, and the fermentation is kept at a constant temperature by a heating device arranged inside the fermentation tank 100, and the motor 102 is further started. The motor 102 drives the gear 103 to rotate at a reduced speed through the gear 2 104, and further drives the rotating shaft 200 to rotate. When the rotating shaft 200 rotates, it can drive the mixing blade 300 and the scraper 202 to rotate. The scraper 202 can reduce the sedimentation at the bottom of the fermentation tank 100, and the internal solution flow can be increased when the mixing blade 300 rotates. At the same time, through the rotation of the rotating shaft 200, the prism shaft 201 and the optical axis 212 rotate synchronously. When the optical axis 212 rotates inside the sleeve 205, the reciprocating thread 213 and the ball inside the sleeve 205 cooperate to drive the prism shaft 201 along the rotating shaft while the prism shaft 201 rotates. The axis of the shaft 200 makes reciprocating motion. Furthermore, the mixing blade 300 arranged on the side wall of the prism shaft 201 can make reciprocating motion in the vertical direction along the axis of the shaft 200 while rotating and stirring around the axis of the shaft 200, which can increase the stirring range of the mixing blade 300, increase the frequency of turbulence generated during rotation, and improve the exchange and mixing effect of the culture solution. It is helpful to break the boundary layer generated by the accumulation of fermentation metabolites of a single bacterial community under the action of centrifugal force or gravity during the fermentation of multiple bacterial communities, avoid sedimentation and agglomeration, and promote the exchange of substances between metabolic materials of different bacterial communities, thereby accelerating the reaction process, maintaining high fermentation efficiency, and improving the production efficiency and economy of composite lipopeptides. At the same time, a more comprehensive and in-depth mixing is obtained in the three-dimensional space inside the fermentation tank 100. With the rotation of the scraper 202, dead corners and local uneven stirring can be avoided.

[0042] Furthermore, when the prism shaft 201 reciprocates in the vertical direction, the meshing of the gear three 305 and the rack 214 can drive the refinement blade 315 to rotate, and further refine and stir the culture solution passing through the surface of the movable blade 301, so as to disperse the material more finely, reduce the local accumulation of the bacterial fermentation process, and improve the mixed fermentation effect of the bacterial community. When the prism shaft 201 moves toward the direction of the liquid pipe 206, the solution inside the pump liquid chamber 203 can be pumped into the liquid supply pipe 209 through the one-way valve inside the sleeve 208 through the piston one 207, and further transported to the supply pipe 307 through the corresponding connecting pipe 303 and sprayed out through the spray hole 310; when the air supply pipe 211 moves toward the sleeve 20 5. When the piston 210 moves in the direction of rotation, the air in the pump air cavity 204 can be pushed by the piston 210 to be pumped to the corresponding connecting pipe 303 through the air supply pipe 211 and sprayed out through the spray hole 310; in the mixed fermentation process, while maintaining the concentration of the culture solution, nutrients and oxygen can be continuously added during the fermentation process. As the fermentation progresses, the appropriate supply of nutrients and oxygen is guaranteed during the fermentation process. Moreover, when nutrients and oxygen are discharged through the spray hole 310, the rotation of the refinement blade 315 is coordinated to make the nutrients quickly and evenly distributed in the culture solution to avoid local concentration differences. At the same time, the rotation and diffusion of the gas can refine the bubbles and improve the dissolution of oxygen, thereby increasing the oxygen uptake efficiency of the bacterial flora.

[0043] At the same time, as the fermentation process proceeds, the polysaccharides produced by the bacterial flora during the fermentation process, including extracellular polysaccharides, and the protein metabolites such as enzymes and polypeptide products produced increase. When the culture liquid product is getting closer and closer to saturation, the viscosity of the culture liquid will increase and the fluidity will deteriorate, so that the resistance of the mixing blade 300 during the rotation of the mixing blade 300 will increase slightly. When the resistance of the mixing blade 300 during rotation increases, the resistance of the movable blade 301 will also increase, causing the movable blade 301 to move toward the inside of the mixing blade 300, and further, causing the rectangular frame 306 to slide inside the sealing groove 302 and move toward the mixing blade 300. The hydraulic medium inside the sealing groove 302 transfers the pressure medium to the inside of the regulating chamber 308 through the hydraulic pipe 312 under the action of pressure. Since the cross-sectional area of ​​the flow inside the regulating chamber 308 is significantly smaller than the area of ​​action between the sealing groove 302 and the rectangular frame 306, when the displacement between the sealing groove 302 and the rectangular frame 306 is small, but the inside of the sealing groove 302 The total amount of hydraulic medium delivered under pressure can push the valve block 316 to move a larger displacement inside the regulating chamber 308, thereby amplifying the monitoring result of the solution state inside the fermentation tank 100. As the valve block 316 moves away from the hydraulic pipe 312, the relative flow cross-sectional area between the connecting groove 314 and the valve groove 313 decreases, so that the speed of gas or nutrients entering the supply pipe 307 through the valve groove 313 becomes lower, thereby reducing the amount of gas and nutrients discharged through the spray hole 310, so that in the early stage of fermentation, sufficient nutrients and oxygen can be supplied to the culture solution of the fermentation tank 100. As the fermentation proceeds, metabolites increase. By detecting the flowability and viscosity changes of the solution and amplifying the detection results, the supply of nutrients and gas to the spray hole 310 is adjusted to be reduced, thereby realizing automatic adjustment of the supply of nutrients and gas to the culture solution according to the fermentation process. While reducing waste and improving resource utilization, it meets the needs of bacteria for nutrients and gas at different stages, and automatically adjusts to adapt to the growth and metabolism of multiple bacteria, effectively improving the economic benefits of mixed fermentation production.

[0044] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. An antibacterial composite lipopeptide mixed fermentation process, characterized in that: The fermentation process adopts a fermentation device comprising a fermentation tank (100) and a motor (102); a rotating shaft (200) is rotatably connected inside the fermentation tank (100); a gear 1 (103) is arranged at the bottom of the rotating shaft (200); a gear 2 (104) is connected to the output end of the motor (102); the gear 2 (104) is meshed with the gear 1 (103); a scraper (202) is arranged on the side wall of the rotating shaft (200); a prismatic shaft (201) is sleeved inside the rotating shaft (200); a mixing blade (300) is arranged on the side wall of the prismatic shaft (201); a transmission shaft (304) is arranged on the surface of the mixing blade (300); and uniformly distributed thinning blades (315) are arranged on the surface of the transmission shaft (304); the fermentation tank (100) is further provided with: A driving mechanism, used for driving the mixing blade (300) to reciprocate in a vertical direction while rotating; A supply mechanism for supplying liquid and oxygen to the interior of the fermentation tank (100) during the fermentation process; A regulating mechanism that adjusts the supply of nutrients and oxygen according to the degree of fermentation; The driving mechanism comprises a support frame (105) arranged inside the fermentation tank (100), a sleeve (205) being fixedly mounted on the top of the support frame (105), an optical axis (212) being arranged at one end of the prismatic shaft (201) extending into the interior of the sleeve (205), a reciprocating thread (213) being arranged on the outer side wall of the optical axis (212), and a ball matched with the reciprocating thread (213) being arranged on the inner side wall of the sleeve (205); The fermentation process comprises the following steps: S1: Inoculate activated single colonies of Bacillus subtilis, Bacillus berberis, and Bacillus amyloliquefaciens into liquid culture medium at a ratio of 1:1:1; S2: mixing the liquid culture medium and putting it into the fermentation tank (100); S3: The fermentation tank (100) is kept at a constant temperature and supplied with sterile air; S4: the motor (102) is started to drive the liquid inside the fermentation tank (100) to mix and ferment through the mixing blades (300); S5: After the fermentation is completed, the fermentation liquid is discharged from the fermentation tank (100), and a crude composite antimicrobial lipopeptide is obtained through operations such as filtration and centrifugation.

2. The antibacterial composite lipopeptide mixed fermentation process according to claim 1, characterized in that: The supply mechanism comprises a pump liquid chamber (203) opened inside the rotating shaft (200); a supply barrel (101) is arranged at the bottom of the fermentation tank (100); a nutrient solution is arranged inside the supply barrel (101); one end of the prism shaft (201) extending into the pump liquid chamber (203) is connected to a piston (207) via a spring; a prism shaft (201) is opened inside the prism shaft (201); a sleeve (208) is sleeved on the prism shaft (201); and the sleeve (208) passes through the piston (207). (207) is connected to the pump liquid chamber (203), the bottom of the pump liquid chamber (203) is provided with a suction tube (206) extending to the inside of the supply barrel (101), the interior of the mixing blade (300) is provided with a connecting tube (303) connected to the liquid supply tube (209), the interior of the mixing blade (300) is movably sleeved with a movable blade (301), the surface of the movable blade (301) is provided with a spray hole (310) that cooperates with the refinement blade (315), and the spray hole (310) is connected to the connecting tube (303).

3. The antibacterial composite lipopeptide mixed fermentation process according to claim 2, characterized in that: A pump air chamber (204) is provided inside the rotating shaft (200), a piston 2 (210) is sleeved on the side wall of the prismatic shaft (201) via a spring, the piston 2 (210) is movably sleeved with the pump air chamber (204), an air supply pipe (211) connected to the pump air chamber (204) is provided inside the prismatic shaft (201), a one-way valve for air intake is provided on the side wall of the pump air chamber (204), two groups of movable blades (301) and corresponding connecting pipes (303) are provided, the air supply pipe (211) is connected to a group of adjacent connecting pipes (303), and the liquid supply pipe (209) is connected to the adjacent connecting pipes (303).

4. The antibacterial composite lipopeptide mixed fermentation process according to claim 2, characterized in that: A gear three (305) is provided at one end of the transmission shaft (304) close to the rotating shaft (200), and a rack (214) cooperating with the sleeve (205) is provided inside the rotating shaft (200).

5. The antibacterial composite lipopeptide mixed fermentation process according to claim 2, characterized in that: The regulating mechanism comprises a sealing groove (302) provided inside the mixing blade (300); a rectangular frame (306) matching the sealing groove (302) is provided on the side wall of the movable blade (301); a spring is provided between the mixing blade (300) and the movable blade (301); a supply pipe (307) communicating with the spray hole (310) is provided on the side wall of the movable blade (301); a regulating chamber (308) is provided on the side wall of the movable blade (301); one end of the supply pipe (307) located inside the mixing blade (300) extends into the inside of the regulating chamber (308); a valve block (316) is sleeved inside the regulating chamber (308); the valve block (316) is sleeved with the supply pipe (307); the valve block (316) is provided with an output pipe (311) communicating with the supply pipe (307); the output pipe (311) is sleeved with the movable pipe (309).

6. The antibacterial composite lipopeptide mixed fermentation process according to claim 5, characterized in that: The movable tube (309) is of L-shaped design, one end of the movable tube (309) is movably connected to the output tube (311), and the other end of the movable tube (309) is movably connected to the connecting tube (303).

7. The antibacterial composite lipopeptide mixed fermentation process according to claim 5, characterized in that: The side wall of the supply pipe (307) is provided with a valve groove (313), and the interior of the valve block (316) is provided with a circle of connecting grooves (314) that cooperate with the output pipe (311) and the valve groove (313).

8. The antibacterial composite lipopeptide mixed fermentation process according to claim 7, characterized in that: The valve groove (313) is designed to be an acute-angled triangle, and the acute-angled side of the valve groove (313) is designed to face a side away from the hydraulic pipe (312).

9. The antibacterial composite lipopeptide mixed fermentation process according to claim 5, characterized in that: A hydraulic pipe (312) is provided at one end of the regulating chamber (308) away from the supply pipe (307); one end of the hydraulic pipe (312) is in communication with the regulating chamber (308), and the other end of the hydraulic pipe (312) is in communication with the interior of the sealing groove (302).

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