Method for improving transplanting survival rate of Machilus zhennan seedlings

Abstract

The invention discloses a method for improving the transplanting survival rate of phoebe zhennan seedlings, and belongs to the technical field of phoebe zhennan planting.The method comprises the steps of seed sand storage, sowing and seedling raising, root cutting treatment, transplanting and the like, phoebe zhennan is transplanted into a container after being sown and placed under a forest for shading and seedling raising, and roots are cut off for transplanting after the phoebe zhennan seedlings are cultivated into the seedlings; a root-relaxing agent is cooperatively applied in the transplanting process and can induce root systems of the machilus zhennan seedlings after root breaking to extend all around and grow well, so that the problem that the root systems of the machilus zhennan container seedlings of the multi-root-system tree species are coiled and wound is solved, then absorption of moisture and nutrients and respiratory metabolism after transplanting are inhibited, the transplanting survival rate of the machilus zhennan seedlings is reduced, and the survival rate of the machilus zhennan seedlings is increased. The later growth condition of the phoebe zhennan seedlings is influenced.

Description

Technical Field

[0001] The invention relates to the technical field of Phoebe zhennan planting, and in particular to a method for improving the survival rate of transplanting large Phoebe zhennan seedlings. Background Art

[0002] Phoebe zhennan, also known as nanmu, is an evergreen tree of the genus Phoebe in the Lauraceae family. It is a species endemic to my country and a national second-level protected endangered plant. Phoebe zhennan has thin twigs, a straight trunk, and is densely covered with gray-brown or gray-yellow soft hairs. It has a hard and dense material with beautiful texture, is dense and hard, resistant to insect infestation, and has a special fragrance. It is a valuable timber afforestation species with high economic value and a precious garden greening tree species.

[0003] Container seedling cultivation is a method of growing seedlings in various containers filled with culture medium by growing propagation materials such as seeds or branches. Compared with ordinary ground planting methods, container seedling cultivation has the advantages of short seedling cultivation cycle, convenient water and soil management, excellent seedling quality and short seedling acclimatization period. It is a seedling production technology widely used in countries around the world. However, container seedlings have the disadvantages of weak resistance to diseases and insect pests and high cost. Therefore, in actual production, it is more suitable to cultivate them into large seedlings and then transplant them. During container seedling cultivation, the roots form a complete root ball with well-developed fibrous roots in the container. Under conventional methods, the seedlings are usually transplanted directly with the soil ball. However, Phoebe camphora is a multi-root tree species. During the cultivation of container seedlings, the developed roots are entangled with each other in the container to form an intricate root ball. The roots are closely arranged, which inhibits oxygen circulation and affects the root absorption of water and nutrients, resulting in a lower survival rate after transplantation and poor growth after transplantation.

[0004] Therefore, it is currently necessary to find a method suitable for transplanting Phoebe zhennan seedlings to solve the problem that the roots of large Phoebe zhennan seedlings in containers are entangled with each other and arranged closely, which inhibits oxygen circulation and affects the root absorption of water and nutrients, thereby reducing the survival rate of seedlings after transplanting and resulting in poor growth of seedlings after transplanting. Summary of the invention

[0005] In view of this, the purpose of the present invention is to provide a method for improving the survival rate of large-scale transplanted Phoebe zhennan seedlings, so as to solve the problem that the roots of large-scale Phoebe zhennan seedlings in containers are entangled with each other and arranged closely, which inhibits oxygen circulation and affects the root absorption of water and nutrients, thereby reducing the survival rate of seedlings and causing poor growth of seedlings after transplantation.

[0006] The present invention solves the above technical problems by the following technical means:

[0007] A method for improving the survival rate of transplanting large seedlings of Phoebe zhennan, the method comprising the following steps:

[0008] (1) Seed storage in sand: After the fruits of Phoebe zhennan are harvested, they are placed in a wooden barrel and the peels are removed to obtain the seeds. The seeds are washed with water and then dried. The seeds are then mixed with river sand in a mass ratio of 3:1 and stored in sand. During this period, water is applied to keep the water content of the river sand at 60%;

[0009] (2) Seeding and seedling raising: From the end of March to the beginning of April, the soil in the greenhouse is plowed and broken up, and then the soil is irrigated at 80 kg / m 2 The pretreated soil is obtained by applying compound fertilizer in an amount of 22 to 30 g / m2; the seeds hidden in the sand are taken out, placed in a 0.3 wt% potassium permanganate solution for disinfection for 15 to 30 min, and then washed with water 2 to 3 times to obtain pretreated seeds; the pretreated seeds are dried at 22 to 30 g / m2. 2 The amount of seedlings is sown in rows, with a furrow spacing of 15 to 20 cm and a sowing depth of 1 to 2 cm. The seedlings are maintained according to conventional methods until 3 to 4 true leaves grow out and then transplanted into a container pot filled with a substrate. After being watered thoroughly, the seedlings are placed under the forest for shade cultivation. Watering, fertilization, and pest and disease control are carried out according to conventional methods. The container pots are replaced at a frequency of once a year. After 3 to 5 years of cultivation, large seedlings of Phoebe zhennan are obtained.

[0010] (3) Root cutting: Take the large seedlings of Phoebe zhennan out of the container pot and cut their roots;

[0011] (4) Transplantation: Dig a planting hole, mix the excavated soil with a root relaxant and calcium peroxide to obtain a mixed soil, and fill the mixed soil back into the planting hole at a thickness of 8 to 12 cm. Then, place the root-broken camphor seedlings vertically in the planting hole, straighten the seedlings by hand, and continue to fill in the remaining mixed soil and compact it. Then, apply 0.5 kg of 50 mg / L indoleacetic acid solution to each plant according to the root irrigation method, and then perform maintenance according to conventional methods.

[0012] Furthermore, in the step (2), the matrix in the container pot is obtained by mixing peat soil, loess soil, and calcium magnesium phosphate fertilizer in a mass ratio of (35-40): (40-50): (1-2).

[0013] Furthermore, in the step (2), when the cultivation is carried out under the forest with shade, the tree canopy density is 0.5 to 0.6.

[0014] Further, the root interruption operation in step (3) is specifically as follows:

[0015] Take the large camphor tree seedlings out of the container pot, then cut off 5 to 10 cm wide roots around the root ball and transplant them.

[0016] Furthermore, in the step (4), the mass ratio of the soil to the root relaxant and calcium peroxide in the mixed soil is (15-20): (2-3): (0.1-0.2).

[0017] Furthermore, the Shugen agent comprises the following raw materials:

[0018] Chitosan, 2wt% isopropanol solution, 1,2-nonanediol, diglycolamine, 0.4wt% hydrochloric acid solution, sodium β-glycerophosphate, ammonium bicarbonate, superphosphate, potassium sulfate.

[0019] Further, the preparation method of the Shugen agent is as follows:

[0020] A: Add chitosan to a 2wt% isopropanol solution and stir to disperse, let stand at room temperature for 2-4 hours, then add 1,2-nonanediol, heat to 40-50°C and react for 1-2 hours, cool to room temperature and let stand overnight after the reaction, then adjust the pH to 4.5-5.5 and add diglycolamine, stir at room temperature and react for 2-4 hours, filter to remove the filtrate after the reaction, and then dry to obtain the treated chitosan;

[0021] B: adding the treated chitosan into a 0.4wt% hydrochloric acid solution to dissolve the chitosan to obtain a chitosan solution; adding β-sodium glycerophosphate into 4 times the mass of water to dissolve the β-sodium glycerophosphate solution; at a temperature of 4°C, slowly injecting the β-sodium glycerophosphate solution into the chitosan solution and stirring to react to obtain a sol solution;

[0022] C: Mix ammonium bicarbonate, superphosphate and potassium sulfate to obtain a mixed fertilizer, then add the mixed fertilizer into 3 times the mass of water and stir to dissolve to obtain a mixed fertilizer liquid; add the mixed fertilizer liquid into the sol liquid and stir evenly to obtain a root relaxant, and store it at 4°C for later use.

[0023] The large camphor tree seedlings are rooted and then transplanted. Using the seedlings' roots' tendency to seek fertilizer, water and oxygen, a root-relaxing agent is prepared and mixed with calcium peroxide into the soil. The combined action of the root-relaxing agent and calcium peroxide induces the root-removed seedlings to grow towards the area where the root-relaxing agent is distributed. Specifically, the root-soothing agent uses chitosan as a carrier, reacts with sodium β-glycerophosphate to prepare a temperature-sensitive sol, and fertilizers such as ammonium bicarbonate and superphosphate are added. The root-soothing agent will gel after being mixed into the soil due to its temperature sensitivity, forming a gel that wraps the soil particles and has good water retention. Therefore, the backfill soil mixed with the root-soothing agent can continuously provide water and nutrients. At the same time, the added calcium peroxide continuously hydrolyzes and releases oxygen under the action of water in the gel, and then utilizes the root system's tendency to seek fertilizer, water, and oxygen to allow the roots of the container-cultivated camphor seedlings to stretch in all directions and grow well towards the soil position where the root-soothing agent is located after the roots are broken, thereby promoting the absorption of nutrients and water and the circulation of oxygen in the roots of the seedlings after transplanting, thereby ensuring the survival rate of the transplanted seedlings and their later growth conditions.

[0024] However, the sol prepared by chitosan and β-sodium glycerophosphate has a high gelation temperature. When the soil temperature is low, gelation cannot be carried out, resulting in nutrient loss and failure to provide good water retention. Therefore, the present invention uses 1,2-nonanediol to react with chitosan to bind it to the chitosan molecular chain, change the interaction force between the molecular chains to promote the cross-linking polymerization of the sol, and then reduce the gelation temperature, so that it can be smoothly gelated in soil at a lower temperature and then wrapped on the surface of soil particles to continuously provide water and nutrients.

[0025] In addition, in order to allow the root-soothing agent to be evenly mixed with soil particles to embed them, the present invention also uses diglycolamine to react with chitosan, and regulates the proton transfer ability of chitosan gel during gelation in the soil by changing the charge properties of the chitosan, thereby delaying the formation of chemical bonds in the gel network and extending the gelation time, so that the chitosan sol can better penetrate into the soil particles before gelling and solidifying, ensuring the uniformity of the distribution of the root-soothing agent, thereby promoting the root system to stretch and grow in multiple directions toward the root-soothing agent distribution area, and then ensuring the survival and growth of seedlings by promoting the growth of the root system.

[0026] Furthermore, in step A, the mass ratio of chitosan, 2 wt% isopropanol solution, 1,2-nonanediol and diglycolamine is 0.2:2:(0.001-0.003):(0.002-0.006).

[0027] Furthermore, in step B, the mass ratio of the pretreated chitosan, 0.4 wt% hydrochloric acid solution, and β-sodium glycerophosphate solution is 0.1:5:3.

[0028] Furthermore, in step C, the mass ratio of ammonium bicarbonate, superphosphate, potassium sulfate and sol solution is (0.2-0.4):(0.1-0.2):(0.1-0.2):8.

[0029] Beneficial effects:

[0030] The invention uses chitosan as a raw material to prepare a thermosensitive gelatinizable root relaxant, adds fertilizers such as ammonium bicarbonate into the root relaxant, mixes the root relaxant and calcium peroxide into soil when transplanting large camphor tree seedlings, gels the root relaxant to form a gel that wraps soil particles to obtain mixed soil that is backfilled into a planting hole, and utilizes the fertilizer, water and oxygen attraction of the seedlings to induce the seedling roots to grow toward the surrounding soil area where the root relaxant is distributed, so that the entangled roots can grow better dispersedly after being cut off, so as to ensure the absorption of water and nutrients and the circulation of oxygen in the roots of the seedlings after transplantation, improve the transplantation survival rate of the seedlings, and ensure the good growth condition of the seedlings after transplantation. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1: This is a picture of the cultivation of Phoebe zhennan seedlings under the forest in Example 4 of the present invention;

[0032] Figure 2 : A picture of large Phoebe zhennan seedlings transplanted in experimental group 1 of the present invention. DETAILED DESCRIPTION

[0033] The present invention will be described in detail below with reference to specific embodiments and accompanying drawings:

[0034] Example 1: Preparation of Shugen Agent

[0035] A: Add 0.2 kg chitosan to 2 kg 2 wt% isopropanol solution and stir to disperse. After standing at room temperature for 3 hours, add 0.002 kg 1,2-nonanediol, heat to 45 ° C and react for 1.5 hours. After the reaction is completed, cool to room temperature and stand overnight. Then adjust the pH to 5 and add 0.004 kg diglycolamine. After stirring at room temperature for 3 hours, filter and remove the filtrate after the reaction is completed, and then dry at 60 ° C to obtain the treated chitosan;

[0036] B: 0.1 kg of treated chitosan was added into 5 kg of 0.4 wt% hydrochloric acid solution to dissolve to obtain chitosan solution; β-sodium glycerophosphate was added into 4 times the mass of water to dissolve to obtain β-sodium glycerophosphate solution; 3 kg of β-sodium glycerophosphate solution was slowly injected into the chitosan solution at 4°C and stirred to react to obtain a sol solution;

[0037] C: Mix 0.3kg of ammonium bicarbonate, 0.15kg of superphosphate and 0.15kg of potassium sulfate to obtain a mixed fertilizer, add the mixed fertilizer into 1.8kg of water and stir to dissolve to obtain a mixed fertilizer liquid; add the mixed fertilizer liquid into 8kg of sol liquid and stir evenly to obtain a root relaxant, and store at 4°C for later use.

[0038] Example 2: Preparation of Shugen Agent II

[0039] A: Add 0.2 kg chitosan to 2 kg 2 wt% isopropanol solution and stir to disperse. After standing at room temperature for 2 hours, add 0.001 kg 1,2-nonanediol, heat to 40 ° C and react for 2 hours. After the reaction is completed, cool to room temperature and stand overnight. Then adjust the pH to 4.5 and add 0.002 kg diglycolamine. After stirring at room temperature for 2 hours, filter and remove the filtrate after the reaction is completed, and then dry at 60 ° C to obtain the treated chitosan;

[0040] B: 0.1 kg of treated chitosan was added into 5 kg of 0.4 wt% hydrochloric acid solution to dissolve to obtain chitosan solution; β-sodium glycerophosphate was added into 4 times the mass of water to dissolve to obtain β-sodium glycerophosphate solution; 3 kg of β-sodium glycerophosphate solution was slowly injected into the chitosan solution at 4°C and stirred to react to obtain a sol solution;

[0041] C: Mix 0.2kg of ammonium bicarbonate, 0.1kg of superphosphate and 0.1kg of potassium sulfate to obtain a mixed fertilizer, and add the mixed fertilizer into 1.2kg of water to dissolve it to obtain a mixed fertilizer liquid; add the mixed fertilizer liquid into 8kg of sol liquid and stir evenly to obtain a root relaxant, and store it at 4°C for later use.

[0042] Example 3: Preparation of Shugen Agent III

[0043] A: Add 0.2 kg chitosan to 2 kg 2 wt% isopropanol solution and stir to disperse, let stand at room temperature for 4 hours, then add 0.003 kg 1,2-nonanediol, heat to 50 ° C for 1 hour, cool to room temperature for overnight after the reaction is completed, then adjust the pH to 5.5, add 0.006 kg diglycolamine, stir at room temperature for 4 hours, filter and remove the filtrate after the reaction is completed, and then dry at 60 ° C to obtain the treated chitosan;

[0044] B: 0.1 kg of treated chitosan was added into 5 kg of 0.4 wt% hydrochloric acid solution to dissolve to obtain chitosan solution; β-sodium glycerophosphate was added into 4 times the mass of water to dissolve to obtain β-sodium glycerophosphate solution; 3 kg of β-sodium glycerophosphate solution was slowly injected into the chitosan solution at 4°C and stirred to react to obtain a sol solution;

[0045] C: Mix 0.4kg of ammonium bicarbonate, 0.2kg of superphosphate and 0.2kg of potassium sulfate to obtain a mixed fertilizer, add the mixed fertilizer into 2.4kg of water and stir to dissolve to obtain a mixed fertilizer liquid; add the mixed fertilizer liquid into 8kg of sol liquid and stir evenly to obtain a root relaxant, and store at 4°C for later use.

[0046] Comparative Example 1: Preparation of Shugen Agent

[0047] In contrast to Example 1, the only difference is that 1,2-nonanediol is not added in step A when preparing the root-soothing agent in Comparative Example 1, as shown below:

[0048] A: 0.2 kg chitosan was added to 2 kg 2 wt% isopropanol solution and stirred and dispersed, and then allowed to stand at room temperature for 3 hours, and then the pH was adjusted to 5 and 0.004 kg diglycolamine was added, and the reaction was stirred at room temperature for 3 hours. After the reaction was completed, the filtrate was removed by filtration, and then dried at 60°C to obtain the treated chitosan;

[0049] B~C: Same as Example 1.

[0050] Comparative Example 2: Preparation of Shugen Agent

[0051] In contrast to Example 1, the only difference is that in the preparation of the root-soothing agent in Comparative Example 2, diglycolamine is not added in step A, as shown below:

[0052] A: Add 0.2 kg chitosan into 2 kg 2 wt% isopropanol solution and stir to disperse. After standing at room temperature for 3 hours, add 0.002 kg 1,2-nonanediol, heat to 45°C and react for 1.5 hours. After the reaction is completed, cool to room temperature and stand overnight. Then filter and remove the filtrate, and dry at 60°C to obtain the treated chitosan.

[0053] B~C: Same as Example 1.

[0054] Comparative Example 3: Preparation of Shugen Agent

[0055] In contrast to Example 1, the only difference is that the original step A is missing in the preparation of the root-soothing agent in Comparative Example 3, and chitosan is not treated, but conventional chitosan is used for preparation, as shown below:

[0056] A: 0.1 kg of chitosan was added into 5 kg of 0.4 wt% hydrochloric acid solution to obtain a chitosan solution; β-sodium glycerophosphate was added into 4 times the mass of water to obtain a β-sodium glycerophosphate solution; 3 kg of β-sodium glycerophosphate solution was slowly injected into the chitosan solution at 4°C, and stirred to obtain a sol solution;

[0057] B: Mix 0.3kg of ammonium bicarbonate, 0.15kg of superphosphate and 0.15kg of potassium sulfate to obtain a mixed fertilizer, add the mixed fertilizer into 1.8kg of water and stir to dissolve to obtain a mixed fertilizer liquid; add the mixed fertilizer liquid into 8kg of sol liquid and stir evenly to obtain a root relaxant, and store at 4°C for later use.

[0058] Comparative Example 4: Preparation of Shugen Agent

[0059] In contrast to Example 1, the only difference is that in the preparation of the root-soothing agent in Comparative Example 4, the pH is adjusted to 3.5 in step A, as shown below:

[0060] A: Add 0.2 kg chitosan to 2 kg 2 wt% isopropanol solution and stir to disperse. After standing at room temperature for 3 hours, add 0.002 kg 1,2-nonanediol, heat to 45 ° C and react for 1.5 hours. After the reaction is completed, cool to room temperature and stand overnight. Then adjust the pH to 3.5 and add 0.004 kg diglycolamine. After stirring at room temperature for 3 hours, filter and remove the filtrate after the reaction is completed, and then dry at 60 ° C to obtain the treated chitosan;

[0061] B~C: Same as Example 1.

[0062] Comparative Example 5: Preparation of Shugen Agent

[0063] In contrast to Example 1, the only difference is that in the preparation of the root-soothing agent in Comparative Example 5, the pH is adjusted to 6.5 in step A, as shown below:

[0064] A: Add 0.2 kg chitosan to 2 kg 2 wt% isopropanol solution and stir to disperse. After standing at room temperature for 3 hours, add 0.002 kg 1,2-nonanediol, heat to 45 ° C and react for 1.5 hours. After the reaction is completed, cool to room temperature and stand overnight. Then adjust the pH to 6.5 and add 0.004 kg diglycolamine. After stirring at room temperature for 3 hours, filter and remove the filtrate after the reaction is completed, and then dry at 60 ° C to obtain the treated chitosan;

[0065] B~C: Same as Example 1.

[0066] Comparative Example 6: Preparation of Shugen Agent

[0067] In contrast to Example 1, the only difference is that the amount of pretreated chitosan in step B during the preparation of the root-soothing agent in Comparative Example 6 is 0.05 kg, as shown below:

[0068] A: Same as Example 1;

[0069] B: 0.05 kg of treated chitosan was added into 5 kg of 0.4 wt% hydrochloric acid solution to dissolve to obtain chitosan solution; β-sodium glycerophosphate was added into 4 times the mass of water to dissolve to obtain β-sodium glycerophosphate solution; 3 kg of β-sodium glycerophosphate solution was slowly injected into the chitosan solution at 4°C, stirred and reacted to obtain sol solution;

[0070] C: Same as Example 1.

[0071] Comparative Example 7: Preparation of Shugen Agent

[0072] In contrast to Example 1, the only difference is that the amount of pretreated chitosan in step B during the preparation of the root-soothing agent in Comparative Example 7 is 0.15 kg, as shown below:

[0073] A: Same as Example 1;

[0074] B: 0.15 kg of treated chitosan was added into 5 kg of 0.4 wt% hydrochloric acid solution to dissolve to obtain chitosan solution; β-sodium glycerophosphate was added into 4 times the mass of water to dissolve to obtain β-sodium glycerophosphate solution; 3 kg of β-sodium glycerophosphate solution was slowly injected into the chitosan solution at 4°C and stirred to react to obtain a sol solution;

[0075] C: Same as Example 1.

[0076] Comparative Example 8: Preparation of Shugen Agent

[0077] In contrast to Example 1, the only difference is that in Comparative Example 8, the amount of sodium β-glycerophosphate solution added during the preparation of the root-soothing agent is 2 kg, as shown below:

[0078] A: Same as Example 1;

[0079] B: 0.1 kg of treated chitosan was added into 5 kg of 0.4 wt% hydrochloric acid solution to dissolve to obtain chitosan solution; β-sodium glycerophosphate was added into 4 times the mass of water to dissolve to obtain β-sodium glycerophosphate solution; 2 kg of β-sodium glycerophosphate solution was slowly injected into the chitosan solution at 4°C and stirred to react to obtain a sol solution;

[0080] C: Same as Example 1.

[0081] Comparative Example 9: Preparation of Shugen Agent

[0082] In contrast to Example 1, the only difference is that the amount of β-sodium glycerophosphate solution added during the preparation of the root-soothing agent in Comparative Example 9 is 4 kg, as shown below:

[0083] A: Same as Example 1;

[0084] B: 0.1 kg of treated chitosan was added into 5 kg of 0.4 wt% hydrochloric acid solution to dissolve to obtain chitosan solution; β-sodium glycerophosphate was added into 4 times the mass of water to dissolve to obtain β-sodium glycerophosphate solution; 4 kg of β-sodium glycerophosphate solution was slowly injected into the chitosan solution at 4°C, stirred and reacted to obtain sol solution;

[0085] C: Same as Example 1.

[0086] Embodiment 4: Method for transplanting large seedlings of Phoebe zhennan:

[0087] (1) Seed storage in sand: After the fruits of Phoebe zhennan are harvested, they are placed in a wooden barrel and the peels are removed to obtain the seeds. The seeds are washed with water and then dried. The seeds are then mixed with river sand in a mass ratio of 3:1 and stored in sand. During this period, water is applied to keep the water content of the river sand at about 60%.

[0088] (2) Seeding and seedling raising: In early April, the soil in the greenhouse was plowed and raked, and the soil was irrigated at 80 kg / m 2 The pretreated soil was obtained by applying compound fertilizer in an amount of 25 g / m 2The amount of seedlings is sown in rows, the furrow spacing is about 16 cm, the sowing depth is about 1.5 cm, and management and maintenance are carried out according to conventional methods; transplanting is carried out after 3 to 4 true leaves grow out. Specifically, peat soil, loess soil, and calcium magnesium phosphate fertilizer are first mixed in a mass ratio of 35:40:1 to obtain a matrix, and the matrix is ​​put into a container pot, and then the seedlings are transplanted into the container pot with the matrix at a rate of one plant per pot, and after being watered thoroughly, they are placed under a fir tree with a canopy density of about 0.5 for shade cultivation, and watering, fertilization, and pest and disease control are carried out according to conventional methods, and the container pot is replaced at a frequency of once a year. After 4 years of cultivation, large Phoebe zhennan seedlings are obtained;

[0089] (3) Root cutting: Take the large seedlings out of the container pot, and then cut off the roots about 6 cm wide around the root ball for transplanting;

[0090] (4) Transplantation: Prepare a root relaxant according to the method of Example 1, then dig a planting hole, mix the excavated soil with the root relaxant and calcium peroxide in a mass ratio of 15:3:0.2 to obtain a mixed soil, and backfill the mixed soil into the planting hole in a thickness of about 10 cm. Then, stand the large seedlings of Phoebe zhennan vertically in the planting hole after the roots are cut off, straighten the large seedlings by hand, and continue to backfill the remaining mixed soil and compact it. Then, apply 0.5 kg of 50 mg / L indoleacetic acid solution to each plant according to the root irrigation method, and perform maintenance according to conventional methods.

[0091] Comparative Example 10: Transplanting method of large seedlings of Phoebe zhennan

[0092] In contrast to Example 4, the only difference is that in Comparative Example 10, root cutting treatment is not performed when transplanting large seedlings of Phoebe zhennan, and transplanting is performed directly, and the remaining steps are the same as those in Example 4.

[0093] Comparative Example 11: Transplanting method of large seedlings of Phoebe zhennan

[0094] In contrast to Example 4, the only difference is that in Comparative Example 11, when transplanting large seedlings of Phoebe zhennan, no root soothing agent is mixed into the soil, but only calcium peroxide is mixed into the soil, and the remaining steps are the same as those of Example 4.

[0095] Comparative Example 12: Transplanting method of large seedlings of Phoebe zhennan

[0096] In contrast to Example 4, the only difference is that in Comparative Example 12, calcium peroxide is not mixed into the soil when transplanting large Phoebe zhennan seedlings, and the remaining steps are the same as in Example 4.

[0097] Comparative Example 13: Transplanting method of large seedlings of Phoebe zhennan

[0098] In contrast to Example 4, the only difference is that in Example 13, the large seedlings of Phoebe zhennan were not subjected to root cutting treatment during transplantation, and the large seedlings were directly taken out of the container for transplantation, and no root relaxant and calcium peroxide were mixed into the soil. The remaining steps were the same as in Example 4.

[0099] Experiment 1: Shugen agent performance test experiment

[0100] 1. The gelation time and gelation temperature of the Shugen agent prepared in Example 1 and Comparative Examples 1 to 9 were tested, and the data obtained are shown in Table 1:

[0101] Table 1

[0102] Gelation temperature (℃) Gel time (s) Example 1 23 326 Comparative Example 1 29 312 Comparative Example 2 23 224 Comparative Example 3 32 209 Comparative Example 4 24 302 Comparative Example 5 25 326 Comparative Example 6 28 408 Comparative Example 7 25 347 Comparative Example 8 27 411 Comparative Example 9 26 375

[0103] According to the data analysis in Table 1:

[0104] (1) The gelation temperature of the root-soothing agent prepared in Example 1 is 23° C., and the gelation time is 326 s, indicating that the root-soothing agent prepared according to the method of the present invention can also effectively gel and wrap the soil in a lower environment, thereby fixing nutrients, water and oxygen-releasing substance calcium peroxide, continuously providing water, nutrients and oxygen, thereby inducing the root system to extend in all directions, and the gelation time is relatively moderate. When mixed with the soil, it can be evenly dispersed in the soil for gelation, thereby inducing the root system to extend in all directions.

[0105] (2) In comparative example 1, 1,2-nonanediol was not added during the preparation of the root-soothing agent, and the gelation temperature was high, so it needed to be applied under relatively high temperature conditions; in comparative example 2, diglycolamine was not added during the preparation of the root-soothing agent, and the gelation time of the root-soothing agent was short, which affected the uniformity of distribution in the soil when mixed with the soil, and reduced the inducing effect of the root-soothing agent on the root expansion growth.

[0106] Experiment 2: Transplantation experiment of large seedlings of Phoebe zhennan

[0107] A large-scale transplanting experiment of Phoebe zhennan seedlings was conducted in the National Long-term Scientific Research Base of Chongqing Academy of Forestry Sciences. The transplanting was carried out in early June. The seedlings were sown and raised according to the methods of steps (1) and (2) in Example 4 and then moved to the ground under the fir forest to cultivate large-scale Phoebe zhennan seedlings. After 4 years of cultivation, large-scale seedlings with the same growth conditions were selected for transplanting. The experiment was divided into 14 groups: experimental group 1 and control groups 1 to 13. The root soothing agents and transplanting methods used in each group were as follows:

[0108] Experimental group 1: the root-soothing agent prepared by the method of Example 1 and the transplanting method of Example 4;

[0109] Control groups 1 to 9: using the root soothing agents of comparative examples 1 to 9 and the transplanting method of embodiment 4 respectively;

[0110] Control group 10: using the root soothing agent of Example 1 and the transplanting method of Comparative Example 10;

[0111] Control group 11: no root soothing agent was used, and the transplanting method of comparative example 11 was used;

[0112] Control group 12: the root-relieving agent prepared by the method of Example 1 and the transplanting method of Comparative Example 12;

[0113] Control group 13 (blank control): no root soothing agent was applied, and the transplanting method of comparative example 13 was adopted.

[0114] 2. Each group transplanted 10 large Phoebe zhennan seedlings with the same growth conditions, and then recorded the transplant survival rate of each group, as well as the growth of the height and the ground diameter of each group of seedlings one year after transplanting. The experiment was repeated three times, and the average data were shown in Table 2:

[0115] Table 2

[0116]

[0117]

[0118] According to the data in Table 2:

[0119] (1) The survival rate of large Phoebe zhennan seedlings in experimental group 1 was high, reaching 100%, and the growth condition was good after transplantation. Since large seedlings were transplanted, the transplantation survival rate between the control groups 1 to 12 and the experimental group 1 was not much different, but the growth amount of plant height and ground diameter in the later period was significantly different. This shows that the method of the present invention can be used to cultivate and transplant large Phoebe zhennan seedlings, while ensuring the survival rate of transplantation, effectively promote the growth of the root system of large Phoebe zhennan seedlings, thereby improving the growth of the plants and promoting the increase of plant height and ground diameter.

[0120] (2) When the blank control group was transplanted with Phoebe zhennan, no root cutting treatment was performed, and no root relaxing agent and calcium peroxide were applied. The survival rate and plant height growth condition were significantly deteriorated, indicating that root cutting treatment was performed on large Phoebe zhennan seedlings according to the method of the present invention, and the root relaxing agent and calcium peroxide prepared by the present invention were mixed into the soil, which could better ensure the transplant survival rate of large Phoebe zhennan seedlings and promote the good growth of the seedlings after transplantation.

[0121] The above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the present invention. Although the present invention is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the purpose and scope of the technical solutions of the present invention, which should be included in the scope of the claims of the present invention. The techniques, shapes, and structural parts not described in detail in the present invention are all known technologies.

Claims

1. A method for improving the survival rate of transplanting large seedlings of Phoebe zhennan, characterized in that: The method is as follows: (1) Seed storage in sand: After picking the fruits of Phoebe zhennan, remove the peel to obtain the seeds, wash the seeds with water, dry them, and then store them in sand for later use; (2) Seeding and seedling raising: From the end of March to the beginning of April, the soil in the greenhouse is plowed and compound fertilizer is applied to obtain pre-treated soil; The seeds stored in the sand are taken out and placed in a 0.3 wt% potassium permanganate solution for disinfection, and then washed with water to obtain pretreated seeds; The pretreated seeds are sown in the pretreated soil in a row sowing manner, and are managed and maintained according to conventional methods until 3 to 4 true leaves grow out, and then transplanted into a container pot filled with a substrate, and then placed under the forest for shade cultivation, and are managed and maintained according to conventional methods, and the container pot is replaced at a frequency of once a year, and large seedlings of Phoebe zhennan are obtained after 3 to 5 years of cultivation; (3) Root cutting: Take the large seedlings of Phoebe zhennan out of the container pot and cut their roots; (4) Transplantation: Dig a planting hole, mix the excavated soil with a root relaxant and calcium peroxide to obtain a mixed soil, and fill the mixed soil back into the planting hole at a thickness of 8 to 12 cm. Then, place the root-cut Phoebe camphora seedlings vertically in the planting hole, and continue to fill the remaining mixed soil and compact it. Then, use the root irrigation method to apply 0.5 kg of 50 mg / L indoleacetic acid solution to each plant, and then perform maintenance according to conventional methods.

2. A method for improving the survival rate of transplanting large seedlings of Phoebe zhennan according to claim 1, characterized in that: In the step (2), the substrate in the container pot is obtained by mixing peat soil, loess soil, and calcium magnesium phosphate fertilizer in a mass ratio of (35-40): (40-50): (1-2).

3. A method for improving the survival rate of transplanting large seedlings of Phoebe zhennan according to claim 2, characterized in that: In the step (2), when the understory shade cultivation is performed, the tree canopy density is 0.5 to 0.

6.

4. A method for improving the survival rate of transplanting large seedlings of Phoebe zhennan according to claim 3, characterized in that: The step (3) of interrupting the root operation is specifically as follows: Take the large camphor tree seedlings out of the container pot, then cut off 5 to 10 cm wide roots around the root ball and transplant them.

5. A method for improving the survival rate of transplanting large seedlings of Phoebe zhennan according to claim 4, characterized in that: In the step (4), the mass ratio of soil to root relaxant and calcium peroxide in the mixed soil is (15-20): (2-3): (0.1-0.2).

6. A method for improving the survival rate of transplanting large seedlings of Phoebe zhennan according to claim 5, characterized in that: The Shugen agent includes the following raw materials: Chitosan, 2wt% isopropanol solution, 1,2-nonanediol, diglycolamine, 0.4wt% hydrochloric acid solution, sodium β-glycerophosphate, ammonium bicarbonate, superphosphate, potassium sulfate.

7. A method for improving the survival rate of transplanting large seedlings of Phoebe zhennan according to claim 6, characterized in that: The preparation method of the Shugen agent is as follows: A: Add chitosan to a 2wt% isopropanol solution and stir to disperse, let stand at room temperature for 2-4 hours, then add 1,2-nonanediol, heat to 40-50°C and react for 1-2 hours, cool to room temperature and let stand overnight after the reaction, then adjust the pH to 4.5-5.5 and add diglycolamine, stir at room temperature and react for 2-4 hours, filter to remove the filtrate after the reaction, and then dry to obtain the treated chitosan; B: adding the treated chitosan into a 0.4wt% hydrochloric acid solution to dissolve the chitosan to obtain a chitosan solution; adding β-sodium glycerophosphate into 4 times the mass of water to dissolve the β-sodium glycerophosphate solution; at a temperature of 4°C, slowly injecting the β-sodium glycerophosphate solution into the chitosan solution and stirring to react to obtain a sol solution; C: Mix ammonium bicarbonate, superphosphate and potassium sulfate to obtain a mixed fertilizer, then add the mixed fertilizer into 3 times the mass of water and stir to dissolve to obtain a mixed fertilizer liquid; add the mixed fertilizer liquid into the sol liquid and stir evenly to obtain a root relaxant, and store it at 4°C for later use.

8. A method for improving the survival rate of transplanting large seedlings of Phoebe zhennan according to claim 7, characterized in that: In the step A, the mass ratio of chitosan, 2 wt% isopropanol solution, 1,2-nonanediol and diglycolamine is 0.2:2:(0.001-0.003):(0.002-0.006).

9. A method for improving the survival rate of transplanting large seedlings of Phoebe zhennan according to claim 8, characterized in that: The mass ratio of the pretreated chitosan, 0.4wt% hydrochloric acid solution and β-sodium glycerophosphate solution in step B is 0.1:5:

3.

10. A method for improving the survival rate of transplanting large seedlings of Phoebe zhennan according to claim 9, characterized in that: In the step C, the mass ratio of ammonium bicarbonate, superphosphate, potassium sulfate and sol solution is (0.2-0.4):(0.1-0.2):(0.1-0.2):8.

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