Method for promoting vernalization of rape by using gibberellin

Abstract

The invention belongs to the technical field of plant cultivation, and particularly relates to a method for promoting vernalization of rape by using gibberellin. The invention discloses a method for promoting vernalization of winter rape by utilizing gibberellin, which comprises the following steps: (1) performing constant-temperature light-shielding germination treatment on brassica napus seed SC4 in gibberellin to obtain the brassica napus seed SC4 with white buds; and (2) culturing the brassica napus seed (Brassica napus seed) SC4 with the white buds under the condition of long sunshine. Under the long-day illumination condition, the flowering periods of the cabbage type rape seeds SC4 which are not vernalized are treated with the gibberellin concentration of 0.001 mol / L and 0.002 mol / L, the flowering periods are extremely remarkable, and the flowering periods of the materials are remarkably shortened.

Description

Technical Field

[0001] The invention belongs to the technical field of plant cultivation, and in particular relates to a method for promoting vernalization of rapeseed by utilizing gibberellins. Background Art

[0002] Rapeseed is the most important oil crop in my country, providing more than 55% of the raw materials for domestic edible vegetable oil. The development of rapeseed production is of great strategic significance for maintaining the security of national edible oil supply.

[0003] Brassica napus is the main cultivated type of rapeseed in my country, accounting for more than 90% of the rapeseed planting area. This type of rapeseed originated in Europe. After decades of modern breeding and improvement, Brassica napus has been successfully domesticated from "winter" to "semi-winter", becoming the main type of rapeseed currently promoted for cultivation. Its growth period is about 210 days (Hu JH et al., 2022), but it still needs a long period of low-temperature vernalization before flowering and fruiting.

[0004] The development of modern rapeseed seed industry requires accelerating the speed of generation and purification to improve breeding efficiency, and the speed of the process is mainly restricted by the growth period. During the summer breeding of rapeseed in other places in the north or indoors, there may be a phenomenon of late flowering or delayed flowering of materials with strong winter properties or failure to flower through vernalization, which affects the breeding process. It is necessary to develop simple and easy methods to promote vernalization of rapeseed and improve the efficiency of generation and breeding.

[0005] Gibberellins are a class of tetracyclic diterpenoid plant growth factors that participate in and affect many aspects of plant growth and development, including seed germination, stem and petiole elongation, flower induction, and flower organ development. Gibberellins promote plant growth by initiating the degradation of DELLA proteins, which in turn inhibit the expression of a series of flower bud induction genes, ultimately achieving the result of gibberellins promoting plant flowering.

[0006] The prior art has related research on using a certain concentration of gibberellin to spray flower buds to promote plant vernalization, but it is not feasible in rapeseed field production practice. The present invention treats the seeds with different concentrations of gibberellin during the seed germination stage, which can promote rapeseed to bloom in advance through vernalization under long-day conditions, and provides a set of solutions for solving the problem of flowering sterility in the seed production link of rapeseed hybrids and improving the efficiency of seed production. Summary of the invention

[0007] In order to promote early flowering of rapeseed under long-day conditions, the present invention provides the following technical solutions:

[0008] The present invention provides a method for promoting rapeseed to pass through vernalization by utilizing gibberellins during the germination period, the method comprising the following steps:

[0009] (1) subjecting Brassica napus seeds SC4 to a constant temperature, dark-protected germination treatment in a gibberellin solution to obtain white Brassica napus seeds SC4;

[0010] (2) Cultivating the whitened Brassica napus seeds SC4 under long-day conditions.

[0011] Preferably, in step (1), the concentration of gibberellin is 0.001 mol to 0.002 mol / L.

[0012] Preferably, in step (1), the accession number of Brassica napus seed SC4 is CCTCCNO: P202413.

[0013] Preferably, in step (2), the long-day conditions are carried out in a 18-22° C. light culture room under 18h / 6h long-day culture conditions.

[0014] Beneficial effects of the present invention:

[0015] Under long-day lighting conditions, the flowering period of Brassica napus seeds SC4 that have not been vernalized can be significantly or extremely significantly shortened when treated with gibberellins at concentrations of 0.001mol / L to 0.002mol / L, while the effect of various gibberellins concentrations on shortening the flowering period of the material after vernalization was not significant. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 : Comparison of flowering period of Brassica napus SC4 treated with different gibberellins concentrations; (left: bud stage; right: flowering stage).

[0017] Figure 2 :Comparison of flowering period data of Brassica napus material SC4 under different durations of low temperature treatment and different gibberellin concentrations. DETAILED DESCRIPTION

[0018] In order to make the purpose, technical solutions and advantages of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in combination with the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0019] Materials and methods

[0020] Brassica napus (SC4) seeds were selected for the experiment.

[0021] Brassica napus seed SC4 is deposited in China Center for Type Culture Collection on April 25, 2024. The deposit number is: CCTCC NO: P202413. The deposit location is: Wuhan University, Wuhan, China.

[0022] The seeds of the two materials were planted in different gibberellins A3 (GA3) (BioBomei, CG3131, C 19 H 22 The germination period treatment was carried out in a solution with a concentration of 0.06.

[0023] Cabbage rapeseed SC4 seeds were placed in a culture dish with a diameter of 10 cm and lined with four layers of filter paper. 10 ml of GA3 solution of 0, 0.001, 0.002 and 0.005 mol / L was poured in respectively. 20 plump and healthy rapeseed seeds were placed in each dish. Three dishes of each concentration were used as three replicates. The seeds were placed in a constant temperature culture room at 22°C and protected from light for germination for 24 h until the seeds turned white. Then, the seeds were transferred to blank absorbent filter paper and low-temperature vernalization was carried out in a constant temperature culture box at 4°C for 0 days, 3 days and 6 days respectively. Then, the seeds were moved to a seedling tray. In a light culture room at 22°C and a long-day culture condition of 18h / 6h, the flowering time of the materials under different treatment conditions and different treatment gradients was counted.

[0024] Preparation method of gibberellin solution: 3.464g is dissolved in 10ml 75% alcohol, 1ml is taken and the volume is made up to 100ml to prepare a 0.001mol / L solution; 2ml is taken and the volume is made up to 100ml to prepare a 0.002mol / L solution; 5ml is taken and the volume is made up to 100ml to prepare a 0.005mol / L solution.

[0025] Experimental Results

[0026] Under long-day lighting conditions, the flowering period of Brassica napus seeds SC4 without vernalization was extremely significantly and significantly shortened at gibberellins concentrations of 0.001 mol / L and 0.002 mol / L, respectively, while the effect of various gibberellins concentrations on shortening the flowering period of the materials after vernalization was not significant.

[0027] in conclusion:

[0028] (1) The application scenario of the technology of using 0.001 mol / L gibberellin to promote flowering during the germination period is not very applicable to spring materials with low vernalization requirements, but it has a wider application scenario for winter materials with high vernalization requirements;

[0029] (2) Through repeated experiments, it was found that a concentration of 0.01 mol / L gibberellin had a toxic effect on seed germination, and 0.005 mol / L had the effect of prolonging the flowering period. It is possible that a small amount of toxic effect existed in the early stage, which delayed the growth and flowering of the material. The effect of lower concentrations requires more verification.

[0030] Specific data analysis results: SC4

[0031]

Claims

1. A method for promoting vernalization of rapeseed using gibberellins, characterized in that: The method comprises the following steps: (1) subjecting Brassica napus seeds SC4 to a constant temperature, dark-protected germination treatment in a gibberellin solution to obtain white Brassica napus seeds SC4; (2) Cultivating the whitened Brassica napus seeds SC4 under long-day conditions.

2. The method according to claim 1, characterized in that: In step (1), the concentration of the gibberellin solution is 0.001-0.002 mol / L.

3. The method according to claim 1, characterized in that: In step (1), the accession number of Brassica napus seed SC4 is CCTCC NO: P202413.

4. The method according to any one of claims 1 to 3, characterized in that: In step (2), the long-day conditions are carried out in a light culture room at 18-22° C. and a long-day culture condition of 18h / 6h.

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