Amino acid biological liquid fertilizer and preparation method thereof

Abstract

The invention relates to the technical field of liquid fertilizer preparation, and provides an amino acid biological liquid fertilizer and a preparation method thereof. Comprising the following raw materials in parts by weight: 48-72 parts of waste mixed fermentation liquor, 8-15 parts of a plant extract, 1.2-3.6 parts of calcium superphosphate, 1-2 parts of fulvic acid, 2-6 parts of mineral source potassium fulvate, 1-3 parts of citric acid chelated iron, 1.5-3.5 parts of zinc sulfate, 0.4-0.8 part of manganese sulfate, 0.6-1.5 parts of magnesium chloride, 8-16 parts of sodium lignin sulfonate and 200-400 parts of water. The problems that an existing liquid fertilizer product is high in production cost, general in comprehensive performance and the like are solved, the effect of promoting growth and increasing yield of crops is achieved, and meanwhile the effect of preventing plant diseases and insect pests can be achieved.

Description

Technical Field

[0001] The invention relates to the technical field of liquid fertilizer preparation, and in particular to an amino acid biological liquid fertilizer and a preparation method thereof. Background Art

[0002] In order to maintain and increase the yield of crops in my country, land fertilization is an indispensable link. Traditional agriculture usually uses solid fertilizers, which lead to water waste and soil acidification due to excessive irrigation and fertilization. Promoting the use of liquid fertilizers, comprehensively improving the water production efficiency and fertilizer utilization rate of farmland, and vigorously developing water-fertilizer integration have become the only way to ensure national food security, develop modern water-saving agriculture, transform agricultural development methods, and promote sustainable agricultural development. The so-called liquid fertilizer is a liquid fertilizer formed by dissolving a variety of nutrients in water. It can provide a large number of elements such as nitrogen, phosphorus, potassium, and trace elements required by plants, and has the characteristics of high nutrient content and easy absorption. Compared with solid fertilizers, liquid fertilizers are easier to be absorbed by plant roots, thereby improving the utilization rate of fertilizers.

[0003] For example, patent number CN201010204235.4 discloses a highly efficient biological amino acid liquid fertilizer, which uses 5-aminolevulinic acid (ALA) produced by microbial fermentation as the main active ingredient, and is simultaneously prepared with urea, potassium dihydrogen phosphate, copper sulfate, zinc sulfate, manganese sulfate, magnesium sulfate, boric acid, and ammonium molybdate. The above components are mixed and prepared in the following amounts: ALA 50-500ppm, N+P2O5+K2O 100-300g / L, Cu+Zn+Mn+Mg+B+Mo10-100g / L, and 0.5-1.25% (M / V) of ethylenediaminepentaacetic acid (DTPA) is added as an ion chelating agent, and NaOH is used to adjust the solution pH. The fertilizer produced by this invention is universal, highly efficient, non-toxic, and pollution-free, and is a natural green and environmentally friendly fertilizer.

[0004] For example, patent number CN202111432900.X discloses a liquid organic fertilizer and its preparation method and application. The invention uses fungus bran and water as raw materials for preparation. By fixing the mass volume of fungus bran and water to 1g:4mL~1g:6mL and performing fermentation treatment, it can make full use of the amino acids, proteins and trace elements in the fungus bran, so that the contents of phosphorus, potassium, calcium and magnesium in the prepared organic fertilizer reach 512.5mg / kg, 1356mg / kg, 974mg / kg and 541.7mg / kg respectively. The content of nutrient elements is high, which changes the abundance of microorganisms, increases the effective phosphorus content in the soil, increases soil fertility, promotes plant growth, and alleviates the obstacles of continuous cropping.

[0005] However, the current proportion of domestic liquid fertilizer products is relatively small, the penetration rate is low, and there are problems such as uneven product quality and non-standard quality standards. However, as the development trend of smart agriculture and precision agriculture shifts towards water-fertilizer integration, the market prospects for liquid fertilizer products are particularly broad, and demand will gradually increase. Therefore, it is urgent to develop a liquid fertilizer product that is cheap, has excellent performance, and can be promoted on a large scale. Summary of the invention

[0006] Therefore, in view of the above, the present invention provides an amino acid biological liquid fertilizer to solve the problems of high production cost and general comprehensive performance of existing liquid fertilizer products.

[0007] Another object of the present invention is to provide a method for preparing the amino acid biological liquid fertilizer.

[0008] To achieve the above object, the present invention is implemented by the following technical solutions:

[0009] An amino acid biological liquid fertilizer comprises the following raw materials in parts by weight: 48-72 parts of waste mixed fermentation liquid, 8-15 parts of plant extract, 1.2-3.6 parts of superphosphate, 1-2 parts of humic acid, 2-6 parts of mineral potassium humate, 1-3 parts of citric acid chelated iron, 1.5-3.5 parts of zinc sulfate, 0.6-1.5 parts of magnesium chloride, 0.4-0.8 parts of manganese sulfate, 8-16 parts of sodium lignin sulfonate and 200-400 parts of water.

[0010] A further improvement is that the waste mixed fermentation liquid is prepared according to the following steps:

[0011] (1) Mix the dregs with an appropriate amount of sterile water to ensure that the dregs are fully moistened, then add the complex enzyme, and perform enzymatic hydrolysis at 45 to 55° C. for 5 to 8 hours, then filter with a filter cloth, and set aside the filtered residue;

[0012] (2) washing and drying peanut vines and peanut shells, chopping them, adding bran and stirring to mix evenly, adding activated fermentation bacteria liquid I and sterile water to the raw materials, and performing anaerobically fermentation at room temperature for 15 to 30 days to obtain peanut waste fermentation liquid;

[0013] The filter residue and bran are stirred and mixed evenly in a mass ratio of 2:1, and then activated fermentation bacteria liquid I and sterile water are added, and anaerobically fermented for 10 to 20 days at room temperature to obtain bean dregs fermentation liquid;

[0014] (3) Mixing the peanut waste fermentation liquid and the bean dregs fermentation liquid obtained in step (2), adding fermentation liquid II, fermenting at room temperature for 8 to 15 days, sterilizing, filtering, and obtaining the waste mixed fermentation liquid.

[0015] A further improvement is that the compound enzyme is a mixture of cellulase and pectinase, and the added amount is 2-6% of the total mass of bean dregs and sterile water. The mass ratio of cellulase to pectinase in the compound enzyme is 1:0.3-0.6. The bean dregs and sterile water are mixed in a mass ratio of 1:2-3.

[0016] A further improvement is that the mass ratio of the peanut vine, peanut shell, fermentation liquid I and sterile water is 1:0.5-0.7:0.15-0.35:8-10. The added amount of bran is 60-100% of the total mass of the peanut vine and peanut shell.

[0017] A further improvement is that the mass ratio of the peanut waste fermentation liquid, the bean dregs fermentation liquid and the fermentation bacteria liquid II is 1:0.6-0.8:0.1-0.3.

[0018] A further improvement is that the fermentation liquid I is prepared by culturing Bacillus pumilus and Lactobacillus plantarum to a concentration of 1×10 7 CFU / ml of bacterial solution, and then mixed in a volume ratio of 1:2.

[0019] A further improvement is that the fermentation liquid II is prepared by culturing Aspergillus niger, Candida albicans and Bacillus saccharolyticus to a concentration of 1×10 8 CFU / ml of bacterial solution, and then mixed in a volume ratio of 1.5-2.5:1-2:1.

[0020] A further improvement is that the preservation number of the Bacillus pumilus is CGMCC No.20023, the preservation number of the Lactobacillus plantarum is CGMCC No.10632, the preservation number of the Aspergillus niger is CGMCC No.21466, the preservation number of the Candida sp. is CGMCC No.8682, and the preservation number of the Bacillus syringae is CGMCC No.4519.

[0021] A further improvement is that the plant extract is composed of clove extract, knotweed extract, purslane extract and rubescens extract in a mass ratio of 1:2-4:1.5-3:0.8-1.6.

[0022] The extraction process of the clove extract, knotweed extract, purslane extract and rubescens extract is as follows: fresh clove leaves, knotweed, purslane and rubescens are selected respectively, washed and dried, crushed to 40-60 mesh, added with a mixed solvent 10-15 times the amount of the raw material powder, ultrasonically extracted for 20-40 minutes, then refluxed at a constant temperature for 2-4 hours, and the filtered filtrate was concentrated under reduced pressure to obtain the corresponding extract. The mixed solvent is prepared by compounding ethanol and ethylene glycol dimethyl ether in a mass ratio of 2-4:1.

[0023] The invention discloses a preparation method of amino acid biological liquid fertilizer, which is prepared by the following steps: weighing each component according to weight ratio, then adding superphosphate, humic acid, mineral source potassium humate, citric acid chelated iron, zinc sulfate, manganese sulfate, magnesium chloride and sodium lignin sulfonate into water, stirring and mixing evenly to obtain a mixed solution, then adding the mixed solution into a reactor filled with waste mixed fermentation liquid and plant extract, stirring and mixing evenly to obtain amino acid biological liquid fertilizer, and filling and packaging the finished product.

[0024] By adopting the above technical solution, the beneficial effects of the present invention are:

[0025] 1. Bean dregs are by-products of the processing of soy products. They are rich in nutrients such as protein and carbohydrates, as well as trace substances such as magnesium, potassium, calcium, phosphorus, iron and flavonoids. Although bean dregs are cheap, easy to obtain, and have high nutritional value, there is still no way to fully utilize the bean dregs. Most of the bean dregs are still used as feed or discarded, and even cause environmental pollution. Peanuts are a common nut crop in daily life. The peanut vine stalks after picking peanuts are often regarded as useless waste and are discarded or burned at will, which wastes resources and pollutes the environment. Similarly, peanut shells are not effectively utilized and are generally thrown directly into the trash can.

[0026] The present invention uses bean dregs, peanut vines and peanut shells as main raw materials, and successively uses composite enzymes and two different fermentation bacterial liquids for treatment. The obtained waste mixed fermentation liquid has high nutritional value and is easily absorbed and utilized by plants. Specifically, after being enzymatically hydrolyzed by the bean dregs composite enzyme, the protein in the bean dregs is degraded, so that the content of amino acid nitrogen in the bean dregs is greatly increased. Secondly, the filter residue, peanut vines and peanut shells are subjected to primary fermentation treatment using fermentation bacterial liquid I to reduce the content of cellulose, increase the content of nutrients, and preliminarily decompose and transform them into active substances such as free amino acids and small molecule polypeptides, so as to give full play to the utilization of waste, promote the growth and development of crops, and increase the yield of crops. Finally, the peanut waste fermentation liquid and the bean dregs fermentation liquid are fermented again using fermentation bacterial liquid II to further decompose and metabolize the organic matter in the waste into active molecules that are easily absorbed by crops and soil, thereby improving the quality and yield of crops.

[0027] In short, the organic matter in the waste is utilized to the maximum extent through secondary fermentation, metabolized and decomposed into nutrients needed by crops and soil, and is extremely easy to absorb.

[0028] 2. The present invention adds specific plant extracts to liquid fertilizers, and cooperates with waste mixed fermentation liquid to provide active nutrition for crop growth, and can also prevent pests and diseases. Plant extracts are extracted from cloves, knotweed, purslane, and wintergrass as raw materials. The crushing treatment before extraction can promote the release of active ingredients. The use of specific solvents for extraction can fully release and dissolve the active ingredients, thereby improving the extraction rate of the active ingredients. In addition, clove extract has an excellent transdermal absorption promoting effect, can promote the penetration of other active ingredients, and better play the corresponding role.

[0029] Crop leaves contain a large number of polyphenol compounds, which can chelate metal ions, allowing more metal ions to act on crops, thereby significantly improving the absorption rate of metal ions. Phenolic compounds contain phenolic hydroxyl functional groups. The mixed fermentation liquid of plant extracts and waste materials contains rich amino acid components. Amino acid molecules contain amino and carboxyl functional groups. The presence of amino groups can change the charge distribution and spatial structure of the molecules, and enhance the chelation ability of phenolic hydroxyl groups for metal ions; when there is a carboxyl functional group, it produces a synergistic effect with phenolic hydroxyl groups, enhancing the attraction to metal ions and making the chelation effect better. The mixed fermentation liquid of plant extracts and waste materials also contains functional groups such as thiol, which will also produce a synergistic effect with phenolic hydroxyl groups.

[0030] In summary, the present invention effectively utilizes waste materials such as bean dregs, peanut vines and peanut shells, and metabolizes them into nutrients that are easily absorbed by crops and soil through enzymatic hydrolysis and secondary fermentation processes. On this basis, trace elements such as potassium, iron, zinc and manganese are supplemented to form a liquid fertilizer with low cost and good fertilizer efficiency, which has the effect of promoting the growth and increasing the yield of crops and can also prevent diseases and insect pests. DETAILED DESCRIPTION

[0031] The following will describe the implementation methods of the present invention in detail in conjunction with specific embodiments, so that the implementation process of how the present invention applies technical means to solve technical problems and achieve technical effects can be fully understood and implemented accordingly.

[0032] Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art, and the reagents and products used are all commercially available. The sources, trade names and components of the reagents used are indicated when they first appear.

[0033] Example 1

[0034] An amino acid biological liquid fertilizer comprises the following raw materials in parts by weight: 48 parts of mixed fermentation liquid of waste materials, 8 parts of plant extracts, 1.2 parts of superphosphate, 1 part of humic acid, 2 parts of mineral potassium humate, 1 part of citric acid chelated iron, 1.5 parts of zinc sulfate, 0.4 parts of manganese sulfate, 0.6 parts of magnesium chloride, 8 parts of sodium lignin sulfonate and 200 parts of water.

[0035] The waste mixed fermentation liquid is prepared according to the following steps:

[0036] (1) Mixing bean dregs and sterile water in a mass ratio of 1:2 to ensure that the bean dregs are fully moistened, and then adding a composite enzyme, wherein the composite enzyme is a mixture of cellulase and pectinase in a mass ratio of 1:0.3, and the added amount is 2% of the total mass of the bean dregs and sterile water, and performing an enzymatic hydrolysis reaction at 45° C. for 8 hours, and then filtering with a filter cloth, and the filtered residue is used for later use;

[0037] (2) washing and drying peanut stalks and peanut shells, chopping them, adding bran and stirring to mix evenly, wherein the amount of bran added is 60% of the total mass of the peanut stalks and peanut shells, adding the activated fermentation solution I and sterile water to the raw materials, and anaerobically fermenting them at room temperature for 15 days to obtain peanut waste fermentation solution, wherein the mass ratio of the peanut stalks, peanut shells, fermentation solution I and sterile water is 1:0.5:0.15:8;

[0038] The filter residue and bran were stirred and mixed evenly in a mass ratio of 2:1, and then activated fermentation bacteria liquid I and sterile water were added, and anaerobically fermented for 10 days at room temperature to obtain bean dregs fermentation liquid;

[0039] The fermentation liquid I is prepared by culturing Bacillus pumilus and Lactobacillus plantarum to a concentration of 1×10 7 CFU / ml of bacterial solution, and then mixed in a volume ratio of 1:2;

[0040] (3) Mix the peanut waste fermentation liquid and the bean dregs fermentation liquid obtained in step (2), add fermentation liquid II, ferment at room temperature for 15 days, sterilize, and filter to obtain the waste mixed fermentation liquid. The mass ratio of the peanut waste fermentation liquid, bean dregs fermentation liquid, and fermentation liquid II is 1:0.6:0.1. The fermentation liquid II is obtained by culturing Aspergillus niger, Candida albicans, and Bacillus saccharolyticus to a concentration of 1×10 8 CFU / ml of bacterial solution, and then mixed in a volume ratio of 1.5:1:1.

[0041] The plant extract is composed of clove extract, knotweed extract, purslane extract, and winter grass extract in a mass ratio of 1:2:1.5:0.8. The extraction process of the clove extract is as follows: select fresh clove leaves, dry them after cleaning, crush them to 40 meshes, add a mixed solvent 10 times the amount of the raw material powder, the mixed solvent is compounded by ethanol and ethylene glycol dimethyl ether in a mass ratio of 2:1, ultrasonic extraction for 20 minutes, then constant temperature reflux extraction for 4 hours, and the filtered filtrate is concentrated under reduced pressure to obtain the clove extract. The extraction process of the knotweed extract is as follows: select fresh knotweed, dry them after cleaning, crush them to 50 meshes, add a mixed solvent 12 times the amount of the raw material powder, the mixed solvent is compounded by ethanol and ethylene glycol dimethyl ether in a mass ratio of 3:1, ultrasonic extraction for 30 minutes, then constant temperature reflux extraction for 3 hours, and the filtered filtrate is concentrated under reduced pressure to obtain the knotweed extract. The extraction process of the purslane extract is as follows: fresh purslane is selected, cleaned and dried, crushed to 60 mesh, 15 times the amount of the raw material powder is added, the mixed solvent is prepared by compounding ethanol and ethylene glycol dimethyl ether at a mass ratio of 4:1, ultrasonic extraction is performed for 40 minutes, and then constant temperature reflux extraction is performed for 2 hours, and the filtered filtrate is concentrated under reduced pressure to obtain the purslane extract. The extraction process of the winter grass extract is as follows: fresh winter grass is selected, cleaned and dried, crushed to 60 mesh, 12 times the amount of the raw material powder is added, the mixed solvent is prepared by compounding ethanol and ethylene glycol dimethyl ether at a mass ratio of 3:1, ultrasonic extraction is performed for 30 minutes, and then constant temperature reflux extraction is performed for 3 hours, and the filtered filtrate is concentrated under reduced pressure to obtain the winter grass extract.

[0042] The invention discloses a preparation method of amino acid biological liquid fertilizer, which is prepared by the following steps: weighing each component according to weight ratio, then adding superphosphate, humic acid, mineral source potassium humate, citric acid chelated iron, zinc sulfate, manganese sulfate, magnesium chloride and sodium lignin sulfonate into water, stirring and mixing evenly to obtain a mixed solution, then adding the mixed solution into a reactor filled with waste mixed fermentation liquid and plant extract, stirring and mixing evenly to obtain amino acid biological liquid fertilizer, and filling and packaging the finished product.

[0043] Example 2

[0044] An amino acid biological liquid fertilizer comprises the following raw materials in parts by weight: 60 parts of mixed fermentation liquid of waste materials, 12 parts of plant extracts, 2.4 parts of superphosphate, 1.5 parts of humic acid, 4 parts of mineral potassium humate, 2 parts of citric acid chelated iron, 2.5 parts of zinc sulfate, 0.6 parts of manganese sulfate, 1 part of magnesium chloride, 12 parts of sodium lignin sulfonate and 300 parts of water.

[0045] The waste mixed fermentation liquid is prepared according to the following steps:

[0046] (1) Mixing bean dregs and sterile water in a mass ratio of 1:2.5 to ensure that the bean dregs are fully moistened, and then adding a composite enzyme, wherein the composite enzyme is a mixture of cellulase and pectinase in a mass ratio of 1:0.5, and the added amount is 4% of the total mass of the bean dregs and sterile water, and performing an enzymatic hydrolysis reaction at 50° C. for 6 hours, and then filtering with a filter cloth, and the filtered residue is used for later use;

[0047] (2) washing and drying peanut stalks and peanut shells, and chopping them, adding bran and stirring to mix evenly, wherein the amount of bran added is 80% of the total mass of the peanut stalks and peanut shells, adding the activated fermentation solution I and sterile water to the raw materials, and performing anaerobically fermentation at room temperature for 20 days to obtain peanut waste fermentation solution, wherein the mass ratio of the peanut stalks, peanut shells, fermentation solution I, and sterile water is 1:0.6:0.25:9;

[0048] The filter residue and bran were stirred and mixed evenly in a mass ratio of 2:1, and then activated fermentation bacteria liquid I and sterile water were added, and anaerobically fermented at room temperature for 15 days to obtain bean dregs fermentation liquid;

[0049] The fermentation liquid I is prepared by culturing Bacillus pumilus and Lactobacillus plantarum to a concentration of 1×10 7 CFU / ml of bacterial solution, and then mixed in a volume ratio of 1:2;

[0050] (3) Mix the peanut waste fermentation liquid and the bean dregs fermentation liquid obtained in step (2), add fermentation liquid II, ferment at room temperature for 12 days, sterilize, and filter to obtain the waste mixed fermentation liquid. The mass ratio of the peanut waste fermentation liquid, bean dregs fermentation liquid, and fermentation liquid II is 1:0.7:0.2. The fermentation liquid II is obtained by culturing Aspergillus niger, Candida albicans, and Bacillus saccharolyticus to a concentration of 1×10 8 CFU / ml of bacterial solution, and then mixed in a volume ratio of 2:2:1.

[0051] The plant extract is composed of clove extract, knotweed extract, purslane extract and rubescens extract in a mass ratio of 1:3:2.2:1.2.

[0052] Example 3

[0053] An amino acid biological liquid fertilizer comprises the following raw materials in parts by weight: 72 parts of waste mixed fermentation liquid, 15 parts of plant extract, 3.6 parts of superphosphate, 2 parts of humic acid, 6 parts of mineral potassium humate, 3 parts of citric acid chelated iron, 3.5 parts of zinc sulfate, 0.8 parts of manganese sulfate, 1.5 parts of magnesium chloride, 16 parts of sodium lignin sulfonate and 400 parts of water.

[0054] The waste mixed fermentation liquid is prepared according to the following steps:

[0055] (1) Mixing bean dregs and sterile water in a mass ratio of 1:3 to ensure that the bean dregs are fully moistened, and then adding a composite enzyme, wherein the composite enzyme is a mixture of cellulase and pectinase in a mass ratio of 1:0.6, and the added amount is 6% of the total mass of the bean dregs and sterile water, and performing an enzymatic hydrolysis reaction at 55° C. for 5 h, and then filtering with a filter cloth, and the filtered residue is used for later use;

[0056] (2) washing and drying peanut stalks and peanut shells, chopping them, adding bran and stirring to mix evenly, wherein the amount of bran added is 100% of the total mass of the peanut stalks and peanut shells, adding activated fermentation liquid I and sterile water to the raw materials, and anaerobically fermenting at room temperature for 30 days to obtain peanut waste fermentation liquid, wherein the mass ratio of the peanut stalks, peanut shells, fermentation liquid I and sterile water is 1:0.7:0.35:10;

[0057] The filter residue and bran were stirred and mixed evenly in a mass ratio of 2:1, and then activated fermentation bacteria liquid I and sterile water were added, and anaerobically fermented at room temperature for 20 days to obtain bean dregs fermentation liquid;

[0058] The fermentation liquid I is prepared by culturing Bacillus pumilus and Lactobacillus plantarum to a concentration of 1×10 7 CFU / ml of bacterial solution, and then mixed in a volume ratio of 1:2;

[0059] (3) Mix the peanut waste fermentation liquid and the bean dregs fermentation liquid obtained in step (2), add fermentation liquid II, ferment at room temperature for 8 days, sterilize, and filter to obtain the waste mixed fermentation liquid. The mass ratio of the peanut waste fermentation liquid, bean dregs fermentation liquid, and fermentation liquid II is 1:0.8:0.3. The fermentation liquid II is obtained by culturing Aspergillus niger, Candida albicans, and Bacillus saccharolyticus to a concentration of 1×10 8 CFU / ml of bacterial solution, and then mixed in a volume ratio of 2.5:1.5:1.

[0060] The plant extract is composed of clove extract, herba syringae pumila extract, purslane extract and rubescens herba extract in a mass ratio of 1:4:3:1.6.

[0061] Comparative Example 1

[0062] An amino acid biological liquid fertilizer is different from Example 3 in that peanut waste fermentation liquid and bean dregs fermentation liquid are directly mixed to form waste mixed fermentation liquid, and fermentation liquid is not used for secondary fermentation.

[0063] Comparative Example 2

[0064] An amino acid biological liquid fertilizer, which differs from Example 3 in that: the Bacillus pumilus in the fermentation bacterial liquid I is replaced by Bacillus subtilis of the same mass.

[0065] Comparative Example 3

[0066] An amino acid biological liquid fertilizer, which differs from Example 3 in that the plant lactobacillus in the fermentation bacterial liquid I is replaced with an equal mass of Lactobacillus acidophilus.

[0067] Comparative Example 4

[0068] An amino acid biological liquid fertilizer is different from Example 3 in that: the Aspergillus niger in the fermentation bacterial liquid II is replaced by Candida and Bacillus syringae in proportion, and the total mass remains unchanged.

[0069] Fermentation liquid II is made of Aspergillus niger, Candida albicans, and Bacillus saccharolyticus.

[0070] Comparative Example 5

[0071] An amino acid biological liquid fertilizer is different from Example 3 in that: the Candida in the fermentation bacterial liquid II is replaced by Aspergillus niger and Bacillus saccharolyticus in proportion, and the total mass remains unchanged.

[0072] Comparative Example 6

[0073] An amino acid biological liquid fertilizer is different from Example 3 in that the cold saccharolytic Bacillus in the fermentation bacterial liquid II is replaced by Candida and Aspergillus niger in proportion, and the total mass remains unchanged.

[0074] Comparative Example 7

[0075] An amino acid biological liquid fertilizer, which differs from Example 3 in that the four plant extracts are replaced with Cnidium monnieri extracts of equal mass, and the total mass remains unchanged.

[0076] Comparative Example 8

[0077] An amino acid biological liquid fertilizer, which is different from Example 3 in that the clove extract is replaced by other three plant extracts in proportion, and the total mass remains unchanged.

[0078] Comparative Example 9

[0079] An amino acid biological liquid fertilizer is different from Example 3 in that the Herba Lycopodii extract is replaced with other three plant extracts in proportion, and the total mass remains unchanged.

[0080] Comparative Example 10

[0081] An amino acid biological liquid fertilizer is different from Example 3 in that the purslane extract is replaced by other three plant extracts in proportion, and the total mass remains unchanged.

[0082] Comparative Example 11

[0083] An amino acid biological liquid fertilizer is different from Example 3 in that the Rubescens rubescens extract is replaced with other three plant extracts in proportion, and the total mass remains unchanged.

[0084] Performance Testing

[0085] The cultivation experiment of Chinese cabbage was carried out in the outdoor experimental field, with an area of ​​260m 2 , divided into 10 test groups and 2 control groups, the test groups are marked as 1 to 10, and each test group corresponds to Example 1, Example 2, Example 3, Comparative Example 1, Comparative Example 2, Comparative Example 3, Comparative Example 4, Comparative Example 5, Comparative Example 6, Comparative Example 7, Comparative Example 8, Comparative Example 9, Comparative Example 10, and the amino acid biological liquid fertilizer prepared by Comparative Example 11 is sprayed on the leaf surface of the corresponding test group by foliar spraying, and the application amount is 50 liters / mu of planting area. Control group 1 sprays commercially available plant-derived amino acid liquid fertilizer (Liwei Shuangbao, produced by Taizhou Agricultural Resources Co., Ltd., Zhejiang Province), and control group 2 sprays an equal amount of water, and other field management measures are completely consistent. After 50 days, Chinese cabbage was harvested, and the growth of Chinese cabbage was measured, as shown in Table 1.

[0086] Table 1

[0087]

[0088]

[0089] It can be seen from the test results in Table 1 that the amino acid biological liquid fertilizer prepared by the present invention shows a good growth-promoting and yield-increasing effect on the growth of Chinese cabbage. In the process of preparing the mixed fermentation liquid of waste materials, omitting the operating steps or adjusting and replacing the strains in the fermentation liquid will reduce the efficacy of the liquid fertilizer, indicating that the method steps in the production process are combined with the joint action of each strain to produce a significant positive effect, and changing any aspect cannot reproduce the good technical effect. The addition of plant extracts can improve the fertilizer effect of liquid fertilizer to a certain extent. There is a synergistic effect between the four natural plant extracts. Without any one of them, the growth of Chinese cabbage will show a certain decline. Further experiments found that the growth of Chinese cabbage showed a more substantial decline in the absence of any two or more of them.

[0090] The main insect pests of Chinese cabbage are cabbage worms, cotton bollworms, aphids, white grubs, mole crickets, etc. A Chinese cabbage cultivation experiment was carried out in an outdoor test field to observe the insect control effect of Chinese cabbage. After 50 days, the insect pest incidence rate of Chinese cabbage was calculated according to the following formula: Insect pest incidence rate = Chinese cabbage (plants) infected with insect pests / total number of Chinese cabbage plants × 100%. The experimental results are shown in Table 2.

[0091] Table 2

[0092]

[0093]

[0094] The Cnidium monnieri extract has insecticidal and fungicidal active ingredients, and field efficacy tests have shown that it has significant effects on various scale pests such as cabbage worms, cotton bollworms, beet armyworms, etc. From the test results in Table 2, it can be seen that the four natural plant extracts used in the present invention have a synergistic effect, and the insect repellent effect is better than the known Cnidium monnieri extract (Comparative Example 7).

[0095] The above records are only embodiments of the present invention. Any modification or change made by a person familiar with the present technology using the present invention shall fall within the patent scope claimed by the present invention and shall not be limited to those disclosed in the embodiments.

Claims

1. An amino acid biological liquid fertilizer, characterized in that: The invention comprises the following raw materials in parts by weight: 48-72 parts of mixed fermentation liquid of waste materials, 8-15 parts of plant extract, 1.2-3.6 parts of superphosphate, 1-2 parts of humic acid, 2-6 parts of mineral potassium humate, 1-3 parts of citric acid chelated iron, 1.5-3.5 parts of zinc sulfate, 0.4-0.8 parts of manganese sulfate, 0.6-1.5 parts of magnesium chloride, 8-16 parts of sodium lignin sulfonate and 200-400 parts of water.

2. An amino acid biological liquid fertilizer according to claim 1, characterized in that: The waste mixed fermentation liquid is prepared according to the following steps: (1) Mix the dregs with an appropriate amount of sterile water to ensure that the dregs are fully moistened, then add the complex enzyme, and perform enzymatic hydrolysis at 45-55°C for 5-8 hours, then filter with a filter cloth, and set aside the filtered residue; (2) Wash and dry the peanut vines and peanut shells, chop them into small pieces, add the bran and stir to mix evenly, add the activated fermentation bacteria liquid I and sterile water to the raw materials, and perform anaerobically fermentation at room temperature for 15 to 30 days to obtain peanut waste fermentation liquid; The filter residue and bran are stirred and mixed evenly, and then activated fermentation bacteria liquid I and sterile water are added, and anaerobically fermented for 10 to 20 days at room temperature to obtain bean dregs fermentation liquid; (3) Mix the peanut waste fermentation liquid and bean dregs fermentation liquid obtained in step (2), add fermentation liquid II, and ferment at room temperature for 8 to 15 days. After sterilization and filtration, the waste mixed fermentation liquid is obtained.

3. An amino acid biological liquid fertilizer according to claim 2, characterized in that: The complex enzyme is a mixture of cellulase and pectinase, and the added amount thereof is 2-6% of the total mass of bean dregs and sterile water.

4. An amino acid biological liquid fertilizer according to claim 2, characterized in that: The mass ratio of the peanut vine, peanut shell, fermentation solution I and sterile water is 1:0.5-0.7:0.15-0.35:8-10.

5. An amino acid biological liquid fertilizer according to claim 2, characterized in that: The mass ratio of the peanut waste fermentation liquid, the bean dregs fermentation liquid and the fermentation bacteria liquid II is 1:0.6-0.8:0.1-0.

3.

6. An amino acid biological liquid fertilizer according to claim 2, characterized in that: The fermentation Bacterial solution I is cultured with Bacillus pumilus and Lactobacillus plantarum to a concentration of 1×10 7 CFU / ml of bacterial solution, Then mix them in a volume ratio of 1:

2.

7. An amino acid biological liquid fertilizer according to claim 2, characterized in that: The fermentation liquid II is prepared by culturing Aspergillus niger, Candida albicans and Bacillus saccharolyticus to a concentration of 1×10 8 CFU / ml of bacterial solution, and then mixed in a volume ratio of 1.5-2.5:1-2:

1.

8. The amino acid biological liquid fertilizer according to claim 1, characterized in that: The plant extract is composed of clove extract, knotweed extract, purslane extract and rubescens extract in a mass ratio of 1:2-4:1.5-3:0.8-1.

6.

9. A method for preparing the amino acid biological liquid fertilizer according to any one of claims 1 to 8, characterized in that: The method is prepared according to the following steps: weigh each component according to the weight ratio, then add superphosphate, humic acid, potassium humate from mineral sources, citric acid chelated iron, zinc sulfate, manganese sulfate, magnesium chloride and sodium lignin sulfonate into water, stir and mix evenly to obtain a mixed solution, then add the mixed solution into a reactor containing mixed fermentation liquid of waste materials and plant extracts, stir and mix evenly to obtain amino acid biological liquid fertilizer, and then fill and package the finished product.

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