Preparation method of intercellular substance-containing artificial tissue

By introducing phenylborate groups into protein vesicles and using polysaccharide aggregate droplets as cell interstitial to form a condensate phase with a similar structure in biological tissue, the problem of artificial cell materials lacking cell interstitial is solved, the enrichment of biological enzymes and the activation of enzymatic reactions is achieved, and the application optimization solution is provided.

CN119746149AInactive Publication Date: 2025-04-04HARBIN INST OF TECH
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Patent Information

Application Number
CN202411972909.3
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-12-30
Publication Date
2025-04-04
Estimated Expiration
Not applicable · inactive patent

AI Technical Summary

Technical Problem

The existing artificial cell materials have a single composition, simple application strategies, and lack of intercellular mass, which limits their similarity and functional development with biological tissues.

Method used

By introducing a phenylborate group on the protein vesicles and using polysaccharide agglomerate microdroplets as cell interstitial, the phenylborate protein vesicles are adhered together to form a condensate phase with a microstructure similar to that of biological tissues.

Benefits of technology

It realizes the formation of a stable condensed phase on the macroscopic scale, and through the ability to enrich biological enzymes, the dormant enzymatic reaction at low concentrations is reactivated, providing optimization ideas for saving dosage, enrichment and recycling and regulating enzyme activity.

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Abstract

The invention relates to a preparation method of an intercellular substance-containing artificial tissue, which comprises the following steps: introducing a phenylboronic acid group on a protein vesicle by a method of copolymerization of N-isopropylacrylamide and 3-acrylamido phenylboronic acid; the preparation method comprises the following steps: constructing aggregate micro-droplets by taking polydiene dimethyl ammonium chloride and carboxylated amylose as primitives; based on a biological orthogonal reaction of phenylboronic acid and polysaccharide, polysaccharide aggregate droplets are used as intercellular substances to adhere phenylboronic acid modified protein vesicles together to form an aggregate phase with a microstructure similar to that of a biological tissue. According to the method, the problems that the aggregate microdroplets cannot stably exist in an aqueous solution for a long time and the application of the aggregate microdroplets is limited to the micro-scale are solved, a stable aggregate phase can be formed on the macro-scale, and biological enzyme reaction under low concentration is enriched and activated; optimization thoughts in the aspects of consumption saving, enrichment and recovery, enzyme activity regulation and control and the like are provided for some high-value biological enzymes in the using process.
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