Novel mutations that increase DNA cleavage activity of amino acid coccus cpF1
By introducing AsCpf1 variants M537R, F870L and M537R/F870L and adding nuclear localization signals, the problem of low enzyme activity in existing Cas12a system was solved, significantly improving its gene editing efficiency in eukaryotic cells.
CN119899824APending Publication Date: 2025-04-29INTEGRATED DNA TECHNOLOGIES INC
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Patent Information
- Application Number
- CN202411279114.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Priority Date
- 2019-07-03
- Filing Date
- 2019-08-08
- Publication Date
- 2025-04-29
AI Technical Summary
Technical Problem
The low enzyme activity of the existing Cas12a system limits its application in genome editing, especially at sites that are difficult to contact with the Cas9 system.
Method used
By introducing AsCpf1 variants M537R, F870L and M537R/F870L, their PAM compatibility is broadened, and the expression and function of Cas12a protein in eukaryotic cells is improved by adding nuclear localization signals.
Benefits of technology
The gene editing efficiency of Cas12a at non-canonical TTTN PAM sites has been significantly improved, and its application potential in eukaryotic cells has been enhanced.
✦ Generated by Eureka AI based on patent content.
Abstract
The present disclosure relates to polynucleotides and amino acids of the Aminococcus sp. Cas12a (Cpf1) and methods for their use in genome editing in eukaryotic cells.
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