Transformation method of ginsenoside in ginseng adventitious roots

Abstract

The embodiment of the invention provides a method for converting ginsenoside in ginseng adventitious roots, which comprises the following steps: (a) taking liquid-cultured ginseng adventitious roots as raw materials, taking the ginseng adventitious roots out of a liquid culture medium, cleaning and removing the residual liquid culture medium on the surfaces of the ginseng adventitious roots, and removing moisture on the surfaces of the ginseng adventitious roots; and (b) suspending the ginseng adventitious roots obtained in the step (a) in high-temperature and high-pressure equipment, adding water into the bottom of the high-temperature and high-pressure equipment, heating to generate high-temperature and high-pressure steam to carry out high-temperature and high-pressure conversion on the ginseng adventitious roots, and controlling reaction parameters to obtain finished ginseng adventitious roots with the rare ginsenoside content obviously improved.

Description

Technical Field

[0001] The present invention relates to the field of plant tissue culture technology, and in particular to a method for transforming ginsenosides in ginseng adventitious roots. Background Art

[0002] Ginseng (Panax ginseng CAMeyer) is a plant of the genus Panax in the Araliaceae family. It is known as the "king of herbs" and has a wide range of applications, covering many fields such as food, medicine, cosmetics and health products. Due to the widespread use of ginseng, the destruction of the growing environment and excessive mining, wild ginseng resources are almost extinct. Ginseng tissue culture technology, especially ginseng adventitious root culture technology, has been gradually applied to the production of ginseng and its bioactive ingredients. Compared with natural ginseng, ginseng adventitious roots cultivated by tissue culture technology have outstanding performance in growth rate and accumulation of bioactive ingredients, and the production process of this technology is stable, avoiding the risks of insect pests, agricultural residues and heavy metal pollution.

[0003] Ginsenosides are the main active ingredients of ginseng adventitious roots and belong to the triterpenoid glycoside compounds. Among the total ginsenosides, more than 60% are ginsenosides of ginseng diol type, including Rg2, Rb1, Rb2, Rc and Rd. After these ginsenosides are treated by physical, chemical and biological transformation methods, some sugar chains are degraded or the side chain at the C-17 position is changed, thus generating rare ginsenosides with extremely low content in the original plant, such as Rg3, Rh2 and Rg5. Compared with the prototype ginsenosides, rare ginsenosides show more significant pharmacological activities such as anti-tumor, liver protection and protection of the nervous system, and have broader application prospects.

[0004] CN202110061909 discloses a method for converting ginsenosides in ginseng adventitious roots, using an alkali degradation method, using one of sodium carbonate, sodium hydroxide, and sodium bicarbonate, to convert a small amount of conventional saponins in adventitious roots into rare saponins, but there is a risk of alkali pollution; CN202311846016 discloses a method for increasing the content of saponins in ginseng adventitious roots and the conversion rate of rare saponins, using a microbial conversion method, using metal chelates and birch inonotus to increase the yield of rare saponins, but there are problems of microbial contamination and antibiotic residues. The production method of rare saponins can also use ginseng as a raw material, after extraction, chemical or biological transformation, but the production process uses a large amount of organic solvents and acid-base substances, and has a complex extraction process, which increases production costs.

[0005] Therefore, it is urgent to propose a green, environmentally friendly, simple and effective method for transforming ginsenosides in ginseng adventitious roots. Summary of the invention

[0006] One or more embodiments of the present specification provide a method for converting ginsenosides in ginseng adventitious roots, comprising: (a) taking liquid-cultured ginseng adventitious roots as raw materials, removing the ginseng adventitious roots from the liquid culture medium, washing and removing the liquid culture medium remaining on the surface of the ginseng adventitious roots, and removing the moisture on the surface of the ginseng adventitious roots; (b) suspending the ginseng adventitious roots obtained in step (a) in a high-temperature and high-pressure device, adding water to the bottom of the high-temperature and high-pressure device, heating to generate high-temperature and high-pressure steam to perform high-temperature and high-pressure conversion on the ginseng adventitious roots, and obtaining a finished ginseng adventitious roots with significantly increased rare ginsenoside content by controlling the reaction parameters.

[0007] In some embodiments, after removing the moisture from the surface of the ginseng adventitious roots, the water content of the ginseng adventitious roots is 80%-90%.

[0008] In some embodiments, the mass ratio of the amount of water added to the bottom of the high temperature and high pressure equipment to the mass of the ginseng adventitious roots is 1:10-1:30.

[0009] In some embodiments, the high temperature and high pressure conversion temperature of the ginseng adventitious roots is 110-135°C.

[0010] In some embodiments, the high temperature and high pressure conversion pressure of the ginseng adventitious roots is 0.05-0.15 MPa.

[0011] In some embodiments, the high temperature and high pressure transformation time of the ginseng adventitious roots is 2-5 hours.

[0012] In some embodiments, the ginsenosides include conventional ginsenosides including Rg2, Rb1, Rc, Rb2 and Rd, and the rare ginsenosides include Rg3, Rk1, Rg5 and Rh2.

[0013] In some embodiments, the ginsenosides are detected by high performance liquid chromatography, and the ginsenoside monomer reference substance is used as an indicator to calculate the ginsenoside content. DETAILED DESCRIPTION

[0014] As shown in this specification and claims, unless the context clearly indicates an exception, the words "a", "an", "an" and / or "the" do not refer to the singular and may also include the plural. Generally speaking, the terms "comprise" and "include" only indicate the inclusion of the steps and elements that have been clearly identified, and these steps and elements do not constitute an exclusive list. The method or device may also include other steps or elements.

[0015] One or more embodiments of the present specification provide a method for converting ginsenosides in ginseng adventitious roots, comprising: (a) taking liquid-cultured ginseng adventitious roots as raw materials, removing the ginseng adventitious roots from the liquid culture medium, washing and removing the liquid culture medium remaining on the surface of the ginseng adventitious roots, and removing the moisture on the surface of the ginseng adventitious roots; (b) suspending the ginseng adventitious roots obtained in step (a) in a high-temperature and high-pressure device, adding water to the bottom of the high-temperature and high-pressure device, heating to generate high-temperature and high-pressure steam to perform high-temperature and high-pressure conversion on the ginseng adventitious roots, and obtaining a finished ginseng adventitious roots with a significantly increased content of rare ginsenosides by controlling the reaction parameters.

[0016] Ginseng adventitious roots refer to the lateral roots growing from the rhizome of ginseng. Such roots are not directly or indirectly formed by the radicle and have no fixed growth position, so they are called adventitious roots. In some embodiments, ginseng adventitious roots can be formed by tissue culture technology. For example, ginseng adventitious roots can be formed by inducing ginseng callus and differentiation culture.

[0017] In some embodiments, ginseng adventitious roots can be cultured in a variety of ways, such as bioreactor culture, suspension culture, shake flask culture, and liquid culture.

[0018] In some embodiments, ginseng adventitious roots are cultured in liquid culture.

[0019] Liquid culture of ginseng adventitious roots refers to placing ginseng adventitious roots in a specific liquid culture medium for cultivation. Compared with traditional solid culture, liquid culture has the advantages of faster growth rate, higher biomass accumulation and more efficient ginsenoside synthesis.

[0020] In some embodiments, the ginseng adventitious roots are taken out from the liquid culture medium, and various solutions may be used to clean and remove the liquid culture medium remaining on the surface of the ginseng adventitious roots.

[0021] In some embodiments, the liquid culture medium remaining on the surface of the ginseng adventitious roots is removed by washing with purified water. In some embodiments, the liquid culture medium remaining on the surface of the ginseng adventitious roots is removed by washing with deionized water.

[0022] In some embodiments, after washing and removing the liquid culture medium remaining on the surface of the ginseng adventitious roots, the surface moisture of the ginseng adventitious roots can be removed by various methods, such as natural air drying, adsorption, vacuum drying, centrifugation, etc. In some embodiments, centrifugation is used to remove the surface moisture of the ginseng adventitious roots.

[0023] Water content is a key indicator for evaluating the quality of ginseng, and different water contents will affect the efficacy and shelf life of ginseng. In some embodiments, the water content of ginseng adventitious roots can be detected by a variety of methods, such as Karl-Fischer method, gas chromatography, and loss on drying method.

[0024] In some embodiments, the water content of ginseng adventitious roots can be measured by loss on drying method.

[0025] The drying loss method is a commonly used moisture detection method. The sample is dried under specific conditions to evaporate the moisture or other volatile substances in the sample, and then the content of these components is obtained based on the mass change of the sample before and after drying. The drying loss method can accurately understand the moisture content in ginseng and ensure the quality and stability of ginseng adventitious roots.

[0026] In some embodiments, after removing the surface moisture of ginseng adventitious roots, the water content of ginseng adventitious roots can be 80%-90%. In some embodiments, after removing the surface moisture of ginseng adventitious roots, the water content of ginseng adventitious roots can be 75%-90%, 84-88%, or 86%-95%.

[0027] In some embodiments, after removing the surface moisture of the ginseng adventitious roots, the water content of the ginseng adventitious roots may be 80%, 82%, 84%, 86%, 88% or 90%.

[0028] In some embodiments, after removing the moisture on the surface of the ginseng adventitious roots, the moisture content of the ginseng adventitious roots may be 80%, 85% or 90%.

[0029] Ginsenosides are the main active ingredients of ginseng and belong to triterpenoid glycoside compounds. Ginsenosides have antioxidant, anti-inflammatory, immunomodulatory, anti-tumor and other effects. In some embodiments, according to the different aglycone structures, ginsenosides include oleanane type, octillone type and dammarane type, etc. Among them, dammarane type saponins include protopanaxadiol type saponins and protopanaxatriol type saponins.

[0030] In some embodiments, ginsenosides include common ginsenosides and rare ginsenosides.

[0031] As used herein, conventional ginsenosides refer to protopanaxadiol-type saponins, which account for more than 60% of the total ginsenosides in ginseng. In some embodiments, conventional ginsenosides include Rg2, Rb1, Rc, Rb2, and Rd.

[0032] Ginsenoside Rg2 belongs to the dammarane-type tetracyclic triterpenoid protopanaxatriol saponin, which has anti-inflammatory, antioxidant, neuroprotective, and cardiovascular protective effects. In terms of the nervous system, Rg2 shows significant neuroprotective effects, repairing damaged blood-brain barriers, preventing harmful substances from invading the brain, and slowing the development of Alzheimer's disease.

[0033] Ginsenoside Rb1 belongs to the dammarane-type tetracyclic triterpenoid protopanaxadiol saponin, which has the ability to regulate the production of reactive oxygen species (ROS) and nitric oxide (NO) and activate various receptors in cells, thereby exerting the effects of anti-oxidative damage, preventing cardiovascular diseases, and lowering blood sugar.

[0034] Ginsenoside Rc is a sterol molecule in ginseng. Rc has significant pharmacological activities such as anti-inflammatory, antioxidant, anti-tumor, lipid regulation, diabetes prevention and protection of the nervous system. In addition, Rc can alleviate liver cell damage caused by excessive acetaminophen (APAP) by enhancing antioxidant defense mechanisms.

[0035] Ginsenoside Rb2 belongs to the dammarane-type tetracyclic triterpenoid protopanaxadiol saponin, which has immunomodulatory, anti-inflammatory, antioxidant, antiviral and anti-aging effects.

[0036] Ginsenoside Rd belongs to the dammarane-type tetracyclic triterpenoid protopanaxadiol saponin, which has neuroprotective, anti-inflammatory, anti-tumor, immunomodulatory, antioxidant and anti-aging effects.

[0037] Conventional ginsenosides can be converted into rare ginsenosides with extremely low content after physical, chemical and biological transformation. Rare ginsenosides are named because of their low content in ginseng. They are mainly formed by desugaring or dehydrating ginsenosides, and are mainly dammarane-type saponins. Rare ginsenosides have more significant pharmacological activities such as anti-tumor, liver protection, and protection of the nervous system than conventional ginsenosides.

[0038] In some embodiments, rare ginsenosides include Rg3, Rk1, Rg5, and Rh2.

[0039] Ginsenoside Rg3 is a dammarane-type tetracyclic triterpenoid protopanaxadiol-type saponin. Rg3 has multiple pharmacological effects such as anti-tumor, anti-inflammatory, antioxidant and immunomodulatory. Rg3 can inhibit the growth, migration and invasion of various cancer cells, such as esophageal squamous cell carcinoma and glioblastoma. Rg3 also has a wide range of other health benefits, including improving cardiovascular health, anti-aging and protecting nerve cells.

[0040] Ginsenoside Rk1 is a rare saponin in ginseng. Rk1 exhibits significant pharmacological activities in anti-inflammatory, anti-tumor, anti-diabetic, and neuroprotective aspects. Among the anti-tumor activities, the rare ginsenoside Rk1 has a significant inhibitory effect on liver cancer.

[0041] Ginsenoside Rg5 belongs to the dammarane-type tetracyclic triterpenoid protopanaxadiol saponin. Rg5 has shown significant pharmacological activities in anti-cancer, anti-inflammatory, memory improvement, and anti-depression.

[0042] Ginsenoside Rh2 belongs to the dammarane-type tetracyclic triterpenoid protopanaxandiol saponin and is a rare saponin in ginseng. Pharmacological studies have shown that Rh2 has a regulatory effect on the immune system, central nervous system, endocrine system, cardiovascular system, etc., and has anti-tumor, anti-allergic, anti-depressant, anti-aging and improvement of myocardial ischemia.

[0043] High temperature and high pressure equipment refers to a device that can safely carry out chemical reactions or physical processes under high temperature and high pressure environments.

[0044] High temperature and high pressure transformation refers to the use of high temperature and high pressure conditions to change the chemical structure of ginsenosides. Under high temperature and high pressure conditions, ginsenoside molecules gain enough energy, and the vibration of chemical bonds within the molecules (such as glycosidic bonds) intensifies, making the chemical bonds easier to break and recombine, thereby promoting the structural transformation of ginsenosides.

[0045] In some embodiments, the mass ratio of the amount of water added to the bottom of the high temperature and high pressure equipment to the ginseng adventitious roots can be 1:10-1:30, 1:05-1:15, 1:16-1:25 or 1:22-1:35.

[0046] In some embodiments, the mass ratio of the amount of water added to the bottom of the high temperature and high pressure equipment to the ginseng adventitious roots can be 1:10, 1:15, 1:20, 1:25 or 1:30. In some embodiments, the mass ratio of the amount of water added to the bottom of the high temperature and high pressure equipment to the ginseng adventitious roots can be 1:10, 1:20 or 1:30.

[0047] In some embodiments, the high temperature and high pressure conversion temperature of ginseng adventitious roots is 110-135°C.

[0048] In some embodiments, the high temperature and high pressure conversion temperature of ginseng adventitious roots may be 110° C., 115° C., 120° C., 125° C., or 135° C. In some embodiments, the high temperature and high pressure conversion temperature of ginseng adventitious roots may be 110° C., 120° C., or 135° C.

[0049] In some embodiments, the high temperature and high pressure conversion pressure of ginseng adventitious roots is 0.05-0.15 MPa.

[0050] In some embodiments, the high temperature and high pressure conversion pressure of ginseng adventitious roots can be 0.05MPa, 0.06MPa, 0.09MPa, 0.10MPa, 0.11MPa, 0.12MPa, 0.13MPa, 0.14MPa or 0.15MPa. In some embodiments, the high temperature and high pressure conversion pressure of ginseng adventitious roots can be 0.05MPa, 0.07MPa, 0.08MPa or 0.15MPa.

[0051] In some embodiments, the high temperature and high pressure transformation time of ginseng adventitious roots is 2-5 hours.

[0052] In some embodiments, the high temperature and high pressure transformation time of ginseng adventitious roots can be 2 hours, 3 hours, 4 hours or 5 hours.

[0053] In some embodiments, the high temperature and high pressure conversion reaction parameters of ginseng adventitious roots may be: conversion temperature 110° C., conversion pressure 0.05 MPa, and conversion time 5 hours.

[0054] In some embodiments, the high temperature and high pressure conversion reaction parameters of ginseng adventitious roots may be: conversion temperature 135° C., conversion pressure 0.15 MPa, and conversion time 2 hours.

[0055] In some embodiments, the high temperature and high pressure conversion reaction parameters of ginseng adventitious roots may be: conversion temperature 120° C., conversion pressure 0.07 MPa, and conversion time 4 hours.

[0056] In some embodiments, the high temperature and high pressure conversion reaction parameters of ginseng adventitious roots may be: conversion temperature 120° C., conversion pressure 0.08 MPa, and conversion time 4 hours.

[0057] In some embodiments, the high temperature and high pressure conversion reaction parameters of ginseng adventitious roots may be: conversion temperature 135° C., conversion pressure 0.15 MPa, and conversion time 3 hours.

[0058] In some embodiments, the content of ginsenosides can be detected by various methods, such as high performance liquid chromatography, liquid chromatography-mass spectrometry, gas chromatography-mass spectrometry, etc.

[0059] In some embodiments, ginsenosides are detected by high performance liquid chromatography, and ginsenoside monomer reference substances are used as indicators to calculate the ginsenoside content.

[0060] In some embodiments of the present specification, liquid-cultured ginseng adventitious roots are used as raw materials, and finished ginseng adventitious roots with significantly increased rare ginsenoside content are obtained through high temperature and high pressure conversion treatment. The steps are simple and stable, without the need to add additional chemical or biological substances, and the conventional ginsenosides in the ginseng adventitious roots are efficiently converted into rare ginsenosides, significantly increasing the content of rare ginsenosides and maintaining a relatively high total saponin recovery rate.

[0061] The following examples are more specific descriptions of some of the above-mentioned embodiments. The partial contents in these embodiments can also be replaced or combined with the corresponding contents in other embodiments, so as to form new embodiments. The experimental methods in the following embodiments are conventional methods unless otherwise specified. The test materials used in the following embodiments are purchased from conventional biochemical reagent companies unless otherwise specified. The quantitative tests in the following embodiments are all set to three repeated experiments, and the results are averaged. It should be understood that the following examples are for better explanation of the present invention and are not intended to limit the present invention. Example Example 1

[0062] 1 kg of ginseng adventitious roots were taken out from the liquid culture medium, washed with pure water to remove the residual liquid culture medium on the surface of the ginseng adventitious roots, centrifuged to remove the surface moisture of the ginseng adventitious roots, and the moisture content was 80% as determined by the drying loss method. The ginseng adventitious roots obtained after centrifugation were suspended in a high temperature and high pressure resistant device, 100 g of water was added to the bottom of the high temperature and high pressure device, and the ginseng adventitious roots were heated to generate high temperature and high pressure steam for high temperature and high pressure conversion, and the reaction parameters were controlled, specifically: the conversion temperature was 110° C., the conversion pressure was 0.05 MPa, and the conversion time was 5 hours.

[0063] The ginsenoside content was determined by high performance liquid chromatography. Before conversion, the total content of the five conventional saponins (Rg2, Rb1, Rc, Rb2 and Rd) was 8.31%. After conversion, the total content of the five conventional saponins was 1.83%, and the conversion rate of conventional saponins was 77.98%. Before conversion, the total content of the four rare saponins was 0.032%. After conversion, the total content of the four rare saponins was 6.31%, an increase of 197 times. Before conversion, the total content of the nine ginsenosides was 8.34%. After conversion, the total content of the nine ginsenosides was 8.14%, and the total saponin yield was 97.60%. Table 1: Determination of saponin content in ginseng adventitious roots Example 2

[0064] 1 kg of ginseng adventitious roots were taken out from the liquid culture medium, washed with pure water to remove the residual liquid culture medium on the surface of the ginseng adventitious roots, centrifuged to remove the surface moisture of the ginseng adventitious roots, and the moisture content was 90% as measured by the drying loss measurement method. The ginseng adventitious roots obtained after centrifugation were suspended in a high temperature and high pressure resistant device, 50 g of water was added to the bottom of the high temperature and high pressure device, and the ginseng adventitious roots were heated to generate high temperature and high pressure steam for high temperature and high pressure conversion, and the reaction parameters were controlled, specifically: the conversion temperature was 135° C., the conversion pressure was 0.15 MPa, and the conversion time was 2 hours.

[0065] The ginsenoside content was determined by high performance liquid chromatography. Before conversion, the total content of the five conventional saponins (Rg2, Rb1, Rc, Rb2 and Rd) was 8.31%. After conversion, the total content of the five conventional saponins was 1.93%, and the conversion rate of conventional saponins was 76.77%. Before conversion, the total content of the four rare saponins was 0.032%. After conversion, the total content of the four rare saponins was 6.14%, an increase of 192 times. Before conversion, the total content of the nine ginsenosides was 8.34%. After conversion, the total content of the nine ginsenosides was 8.07%, and the total saponin yield was 96.76%. Example 3

[0066] 1 kg of ginseng adventitious roots were taken out from the liquid culture medium, washed with pure water to remove the residual culture medium on the surface of the ginseng adventitious roots, centrifuged to remove the surface moisture of the ginseng adventitious roots, and the moisture content was determined to be 85% by the drying loss determination method. The ginseng adventitious roots obtained after centrifugation were suspended in a high temperature and high pressure resistant device, 33 g of water was added to the bottom of the high temperature and high pressure device, and the ginseng adventitious roots were heated to generate high temperature and high pressure steam for high temperature and high pressure conversion, and the reaction parameters were controlled, specifically: the conversion temperature was 120°C, the conversion pressure was 0.07 MPa, and the conversion time was 4 hours.

[0067] The ginsenoside content was determined by high performance liquid chromatography. Before conversion, the total content of the five conventional saponins (Rg2, Rb1, Rc, Rb2 and Rd) was 8.31%. After conversion, the total content of the five conventional saponins was 1.73%, and the conversion rate of conventional saponins was 79.18%. Before conversion, the total content of the four rare saponins was 0.032%. After conversion, the total content of the four rare saponins was 6.32%, an increase of 197 times. Before conversion, the total content of the nine ginsenosides was 8.34%. After conversion, the total content of the nine ginsenosides was 8.05%, and the total saponin yield was 96.52%. Example 4

[0068] 30 kg of ginseng adventitious roots were taken out from the liquid culture medium, washed with pure water to remove the residual culture medium on the surface of the ginseng adventitious roots, centrifuged to remove the surface moisture of the ginseng adventitious roots, and the moisture content was determined to be 85% by the drying loss determination method. The ginseng adventitious roots obtained after centrifugation were suspended in a high temperature and high pressure resistant device, 1 kg of water was added to the bottom of the high temperature and high pressure device, and the ginseng adventitious roots were heated to generate high temperature and high pressure steam for high temperature and high pressure conversion, and the reaction parameters were controlled, specifically: the conversion temperature was 120°C, the conversion pressure was 0.08 MPa, and the conversion time was 4 hours.

[0069] The ginsenoside content was determined by high performance liquid chromatography. Before conversion, the total content of the five conventional saponins (Rg2, Rb1, Rc, Rb2 and Rd) was 9.47%. After conversion, the total content of the five conventional saponins was 2.03%, and the conversion rate of conventional saponins was 78.56%. Before conversion, the total content of the four rare saponins was 0.038%. After conversion, the total content of the four rare saponins was 6.56%, an increase of 172 times. Before conversion, the total content of the nine ginsenosides was 9.51%. After conversion, the total content of the nine ginsenosides was 8.59%, and the total saponin yield was 90.33%. Example 5

[0070] 100 kg of ginseng adventitious roots were taken out from the liquid culture medium, washed with pure water to remove the residual culture medium on the surface of the ginseng adventitious roots, centrifuged to remove the surface moisture of the ginseng adventitious roots, and the moisture content was 80% determined by the drying loss method. The ginseng adventitious roots obtained after centrifugation were suspended in a high temperature and high pressure resistant device, 5 kg of water was added to the bottom of the high temperature and high pressure device, and the ginseng adventitious roots were heated to generate high temperature and high pressure steam for high temperature and high pressure conversion, and the reaction parameters were controlled, specifically: the conversion temperature was 135° C., the conversion pressure was 0.15 MPa, and the conversion time was 3 hours.

[0071] The ginsenoside content was determined by high performance liquid chromatography. Before conversion, the total content of the five conventional saponins (Rg2, Rb1, Rc, Rb2 and Rd) was 9.47%. After conversion, the total content of the five conventional saponins was 1.96%, and the conversion rate of conventional saponins was 79.30%. Before conversion, the total content of the four rare saponins was 0.038%. After conversion, the total content of the four rare saponins was 6.13%, and the content increased by 161 times. Before conversion, the total content of the nine ginsenosides was 9.51%. After conversion, the total content of the nine ginsenosides was 8.09%, and the total saponin yield was 85.07%.

[0072] The basic concepts have been described above. Obviously, for those skilled in the art, the above detailed disclosure is only for example and does not constitute a limitation of this specification. Although not explicitly stated here, those skilled in the art may make various modifications, improvements and corrections to this specification. Such modifications, improvements and corrections are suggested in this specification, so such modifications, improvements and corrections still belong to the spirit and scope of the exemplary embodiments of this specification.

[0073] At the same time, this specification uses specific words to describe the embodiments of this specification. For example, "one embodiment", "an embodiment", and / or "some embodiments" refer to a certain feature, structure or characteristic related to at least one embodiment of this specification. Therefore, it should be emphasized and noted that "one embodiment" or "an embodiment" or "an alternative embodiment" mentioned twice or more in different positions in this specification does not necessarily refer to the same embodiment. In addition, certain features, structures or characteristics in one or more embodiments of this specification can be appropriately combined.

[0074] Similarly, it should be noted that in order to simplify the description disclosed in this specification and thus help understand one or more embodiments of the invention, in the above description of the embodiments of this specification, multiple features are sometimes combined into one embodiment or its description. However, this disclosure method does not mean that the features required by the subject matter of this specification are more than the features mentioned in the claims. In fact, the features of the embodiments are less than all the features of the single embodiment disclosed above.

[0075] In some embodiments, numbers describing the number of components and attributes are used. It should be understood that such numbers used in the description of the embodiments are modified by the modifiers "about", "approximately" or "substantially" in some examples. Unless otherwise specified, "about", "approximately" or "substantially" indicate that the numbers are allowed to vary by ±20%. Accordingly, in some embodiments, the numerical parameters used in the specification and claims are approximate values, which may change according to the required features of individual embodiments. In some embodiments, the numerical parameters should take into account the specified significant digits and adopt the general method of retaining digits. Although the numerical domains and parameters used to confirm the breadth of their range in some embodiments of this specification are approximate values, in specific embodiments, the setting of such numerical values ​​is as accurate as possible within the feasible range.

[0076] Each patent, patent application, patent application publication, and other materials, such as articles, books, specifications, publications, documents, etc., cited in this specification are hereby incorporated by reference in their entirety. Except for application history documents that are inconsistent with or conflicting with the contents of this specification, documents that limit the broadest scope of the claims of this specification (currently or later attached to this specification) are also excluded. It should be noted that if the descriptions, definitions, and / or use of terms in the materials attached to this specification are inconsistent or conflicting with the contents described in this specification, the descriptions, definitions, and / or use of terms in this specification shall prevail.

[0077] Finally, it should be understood that the embodiments described in this specification are only used to illustrate the principles of the embodiments of this specification. Other variations may also fall within the scope of this specification. Therefore, as an example and not a limitation, alternative configurations of the embodiments of this specification may be considered consistent with the teachings of this specification. Accordingly, the embodiments of this specification are not limited to the embodiments explicitly introduced and described in this specification.

Claims

1. A method for converting ginsenosides in ginseng adventitious roots, characterized in that: include: (a) using liquid cultured ginseng adventitious roots as raw materials, removing the ginseng adventitious roots from the liquid culture medium, washing and removing the liquid culture medium remaining on the surface of the ginseng adventitious roots, and removing the moisture on the surface of the ginseng adventitious roots; (b) suspending the ginseng adventitious roots obtained in step (a) in a high-temperature and high-pressure device, adding water to the bottom of the high-temperature and high-pressure device, heating to generate high-temperature and high-pressure steam to perform high-temperature and high-pressure conversion on the ginseng adventitious roots, and obtaining a finished ginseng adventitious roots with significantly increased rare ginsenoside content by controlling reaction parameters.

2. The method for converting ginsenosides in ginseng adventitious roots according to claim 1, characterized in that: After removing the moisture on the surface of the ginseng adventitious roots, the water content of the ginseng adventitious roots is 80%-90%.

3. The method for converting ginsenosides in ginseng adventitious roots according to claim 1, characterized in that: The mass ratio of the water added to the bottom of the high-temperature and high-pressure equipment to the ginseng adventitious roots is 1:10-1:

30.

4. The method for converting ginsenosides in ginseng adventitious roots according to claim 1, characterized in that: The high temperature and high pressure conversion temperature of the ginseng adventitious roots is 110-135°C.

5. The method for converting ginsenosides in ginseng adventitious roots according to claim 1, characterized in that: The high temperature and high pressure conversion pressure of the ginseng adventitious roots is 0.05-0.15 MPa.

6. The method for converting ginsenosides in ginseng adventitious roots according to claim 1, characterized in that: The high temperature and high pressure transformation time of the ginseng adventitious roots is 2-5 hours.

7. The method for converting ginsenosides in ginseng adventitious roots according to claim 1, characterized in that: The ginsenosides include conventional ginsenosides and rare ginsenosides. The conventional ginsenosides include Rg2, Rb1, Rc, Rb2 and Rd. The rare ginsenosides include Rg3, Rk1, Rg5 and Rh2.

8. The method for converting ginsenosides in ginseng adventitious roots according to claim 1, characterized in that: The ginsenosides are detected by high performance liquid chromatography, with ginsenoside monomer reference substances as indicators, and the ginsenoside content is calculated.

Images