High-efficiency growth-promoting pig composite additive and preparation method thereof

Abstract

The invention relates to the technical field of animal feed, in particular to a high-efficiency growth-promoting pig compound additive and a preparation method thereof. The high-efficiency growth-promoting pig compound additive is prepared from the following raw materials in parts by weight: 5-8 parts of bacillus subtilis; 3 to 5 parts of bacillus licheniformis; 4 to 6 parts of fructo-oligosaccharide; 3 to 5 parts of galactooligosaccharide; 8 to 12 parts of yeast extract; 2-4 parts of a yucca extract; 1 to 2 parts of cellulase; 1-2 parts of acid protease; 0.5 to 1.5 parts of curcumin; 0.3 to 0.8 part of tea polyphenol; 1-3 parts of taurine; and 2 to 4 parts of glycine chelated zinc. According to the efficient growth-promoting pig compound additive and the preparation method thereof, multi-dimensional collaborative optimization is adopted, probiotics and prebiotics are combined, colonization of beneficial bacteria in intestinal tracts is promoted, metabolic active substances are generated, antibiotic dependence is replaced, organic microelements are used for replacing high-dose inorganic zinc, and the risk of environment residues is reduced; the natural anti-inflammatory component and the light stabilizer reduce oxidative stress and photosensitive toxicity, are significantly superior to the traditional scheme, and have both environmental friendliness and cost effectiveness.

Description

Technical Field

[0001] The present invention relates to the technical field of animal feed, and particularly to a high-efficiency growth-promoting pig compound additive and a preparation method thereof. Background Art

[0002] Feed additives refer to functional substances added to animal feed, aiming to improve the growth performance, health level and production efficiency of animals by supplementing nutrients, regulating physiological functions or improving feed utilization rate. Its core principles include: supplementing essential nutrients, promoting digestion and absorption, etc.

[0003] However, generally, traditional livestock additives often mainly consist of antibiotics, high-dose inorganic minerals, synthetic hormones and chemical growth promoters. These components have significant disadvantages: long-term use of antibiotics is likely to lead to the emergence of drug-resistant strains, threatening public health safety; excessive inorganic minerals are discharged with feces, polluting soil and water bodies and causing heavy metal accumulation; hormone residues may affect the human endocrine system through the food chain. In addition, chemical additives may inhibit the animal's own immunity, increase disease susceptibility, and have a destructive impact on the intestinal microbial diversity.

[0004] Based on this, the present invention provides a high-efficiency growth-promoting pig compound additive and a preparation method thereof to solve the above-mentioned technical problems. Summary of the Invention

[0005] The purpose of the present invention is to provide a high-efficiency growth-promoting pig compound additive and a preparation method thereof to solve the problems mentioned in the above background art.

[0006] To achieve the above purpose, the present invention provides the following technical solutions:

[0007] The present invention provides a high-efficiency growth-promoting pig compound additive, which is composed of the following raw materials in parts by weight: Bacillus subtilis: 5 - 8 parts; Bacillus licheniformis: 3 - 5 parts; fructooligosaccharide: 4 - 6 parts; galactooligosaccharide: 3 - 5 parts; yeast extract: 8 - 12 parts; yucca extract: 2 - 4 parts; cellulase: 1 - 2 parts; acid protease: 1 - 2 parts; curcumin: 0.5 - 1.5 parts; tea polyphenols: 0.3 - 0.8 parts; taurine: 1 - 3 parts; glycine zinc chelate: 2 - 4 parts.

[0008] Preferably, the Bacillus subtilis is prepared by liquid fermentation, centrifugal concentration and spray drying of the Bacillus subtilis strain in an environment of 30 - 37 °C and pH 6.5 - 7.2, and the purity is that the viable bacteria count is greater than or equal to 1×10^10 CFU / g;

[0009] Preferably, the Bacillus licheniformis is prepared by deep fermentation, centrifugal separation and freeze-drying of the Bacillus licheniformis strain in an environment of 28-35°C and pH 6.8-7.5, and the purity is that the viable bacteria count is greater than or equal to 5×10^9 CFU / g.

[0010] Preferably, the yeast extract is prepared by autolysis, centrifugal concentration and spray drying of brewer's yeast in an environment of 45-55°C and pH 5.0-6.0.

[0011] Preferably, the yucca extract is prepared by reflux extraction, resin purification and drying of yucca stems in an environment of 60-70°C and 60-70% ethanol.

[0012] Preferably, the cellulase is prepared by liquid fermentation, ultrafiltration concentration and carrier adsorption of Trichoderma in an environment of 28-32°C and pH 4.5-5.5.

[0013] Preferably, the taurine is prepared by esterification, neutralization crystallization and decolorization of ethanolamine in an environment of 80-90°C and pH 1-2.

[0014] Preferably, the zinc glycinate chelate is prepared by chelation reaction and spray drying of glycine and zinc sulfate in an environment of 50-60°C and pH 6.5-7.5.

[0015] The present invention also provides a preparation method of a high-efficiency growth-promoting pig compound additive, comprising the following steps:

[0016] S1. Weigh each raw material in a clean workshop with a humidity less than or equal to 30% and a temperature of 25°C according to the formula ratio: 5-8 parts of Bacillus subtilis, 3-5 parts of Bacillus licheniformis, 4-6 parts of fructooligosaccharide, 3-5 parts of galactooligosaccharide, 8-12 parts of yeast extract, 2-4 parts of yucca extract, 1-2 parts of cellulase, 1-2 parts of acid protease, 0.5-1.5 parts of curcumin, 0.3-0.8 parts of tea polyphenols, 1-3 parts of taurine, 2-4 parts of zinc glycinate chelate;

[0017] S2. First, put Bacillus subtilis, Bacillus licheniformis, fructooligosaccharide and galactooligosaccharide into a three-dimensional motion mixer, set the rotation speed at 10-15 rpm and mix for 15 minutes to wrap probiotics with prebiotics to improve stability. Then, add the yeast extract, yucca extract, cellulase and acid protease to another mixing bin and mix for 10 minutes in an environment below 40°C to avoid inactivation of enzyme preparations. Finally, premix curcumin, tea polyphenols, taurine and zinc glycinate chelate;

[0018] S3. Feed the three groups of segmented and premixed materials into a high-efficiency double-helical conical mixer, set the main shaft rotation speed at 25 - 30 rpm, the helical ribbon rotation speed at 100 - 120 rpm, the mixing time at 40 - 60 minutes, and the coefficient of variation of mixing uniformity less than or equal to 5%. Then, conduct micronization treatment on the agglomerated materials through an air-flow pulverizer, control the particle size to be less than or equal to 80 mesh. Finally, use a vibrating screen with an 80 - 100 mesh screen to screen out impurities to ensure the fluidity of the finished powder.

[0019] S4. Transport the mixed materials to a fluidized bed coater, adopt the microencapsulation coating process, with the inlet air temperature at 50 - 60 °C, the material temperature less than or equal to 40 °C, and the survival rate of probiotics after coating greater than or equal to 90%. Then, conduct pre-treatment for light-proof packaging on the photosensitive components of tea polyphenols and curcumin, operate in a red-light illumination environment, and finally remove foreign objects through a metal detector.

[0020] S5. Use an automatic quantitative packaging machine to conduct nitrogen-filled and sealed packaging in an environment with a temperature of 25 °C and a humidity less than or equal to 30%, and then conduct sampling and testing of key indicators.

[0021] Preferably, the key indicators in step S5 include the viable count of probiotics, enzyme activity, and heavy metal residues.

[0022] Compared with the prior art, the beneficial effects of the present invention are:

[0023] The high-efficiency growth-promoting pig compound additive and its preparation method of the present invention are optimized through multi-dimensional coordination. By combining probiotics and prebiotics, it promotes the colonization of beneficial intestinal bacteria and generates metabolically active substances, replaces the dependence on antibiotics, replaces high-dose inorganic zinc with organic trace elements, and reduces the environmental residue risk; natural anti-inflammatory components and light stabilizers reduce oxidative stress and photosensitizing toxicity, significantly superior to traditional solutions, and at the same time have environmental friendliness and cost-effectiveness. Detailed Embodiments

[0024] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without making creative efforts belong to the scope of protection of the present invention.

[0025] I. Materials:

[0026] Unless otherwise stated, each component of the high-efficiency growth-promoting pig compound additive of the present invention is commercially available:

[0027] The present invention provides a highly efficient growth-promoting compound additive for pigs, which is composed of the following raw materials in parts by weight: Bacillus subtilis: 5-8 parts; Bacillus licheniformis: 3-5 parts; fructooligosaccharide: 4-6 parts; galactooligosaccharide: 3-5 parts; yeast extract: 8-12 parts; yucca extract: 2-4 parts; cellulase: 1-2 parts; acid protease: 1-2 parts; curcumin: 0.5-1.5 parts; tea polyphenols: 0.3-0.8 parts; taurine: 1-3 parts; glycine zinc chelate: 2-4 parts.

[0028] Among them, it should be noted that Bacillus subtilis is prepared by liquid fermentation, centrifugal concentration and spray drying of Bacillus subtilis strains in an environment of 30-37 °C and pH 6.5-7.2, and the purity is that the viable bacteria count is greater than or equal to 1×10^10 CFU / g;

[0029] Among them, it should be noted that Bacillus licheniformis is prepared by submerged fermentation, centrifugal separation and freeze drying of Bacillus licheniformis strains in an environment of 28-35 °C and pH 6.8-7.5, and the purity is that the viable bacteria count is greater than or equal to 5×10^9 CFU / g.

[0030] Among them, it should be noted that yeast extract is prepared by autolysis, centrifugal concentration and spray drying of brewer's yeast in an environment of 45-55 °C and pH 5.0-6.0.

[0031] Among them, it should be noted that yucca extract is prepared by reflux extraction, resin purification and drying of yucca stems in an environment of 60-70 °C and 60-70% ethanol.

[0032] Among them, it should be noted that cellulase is prepared by liquid fermentation, ultrafiltration concentration and carrier adsorption of Trichoderma in an environment of 28-32 °C and pH 4.5-5.5.

[0033] Among them, it should be noted that taurine is prepared by esterification, neutralization crystallization and decolorization of ethanolamine in an environment of 80-90 °C and pH 1-2.

[0034] Among them, it should be noted that glycine zinc chelate is prepared by chelation reaction and spray drying of glycine and zinc sulfate in an environment of 50-60 °C and pH 6.5-7.5.

[0035] II. Process:

[0036] Based on the above formula of the highly efficient growth-promoting compound additive for pigs, the present invention also provides a preparation method of the highly efficient growth-promoting compound additive for pigs, which includes the following steps:

[0037] S1. Weigh each raw material in a clean workshop with a humidity less than or equal to 30% and a temperature of 25°C according to the formula ratio: 5 - 8 parts of Bacillus subtilis, 3 - 5 parts of Bacillus licheniformis, 4 - 6 parts of fructooligosaccharide, 3 - 5 parts of galactooligosaccharide, 8 - 12 parts of yeast extract, 2 - 4 parts of yucca extract, 1 - 2 parts of cellulase, 1 - 2 parts of acid protease, 0.5 - 1.5 parts of curcumin, 0.3 - 0.8 parts of tea polyphenols, 1 - 3 parts of taurine, 2 - 4 parts of zinc glycinate chelate;

[0038] S2. First, put Bacillus subtilis, Bacillus licheniformis, fructooligosaccharide, and galactooligosaccharide into a three-dimensional motion mixer, set the rotation speed at 15 rpm and mix for 15 minutes to wrap probiotics with prebiotics to improve stability. Then, add yeast extract, yucca extract, cellulase, and acid protease to another mixing bin and mix for 10 minutes in an environment below 40°C to avoid inactivation of enzyme preparations. Finally, premix curcumin, tea polyphenols, taurine, and zinc glycinate chelate;

[0039] S3. Put the three groups of materials premixed in segments into a high-efficiency double-helix conical mixer, set the main shaft rotation speed at 30 rpm and the helical belt rotation speed at 120 rpm, mix for 60 minutes, and the coefficient of variation of the mixing uniformity is less than or equal to 5%. Then, perform micronization treatment on the agglomerated materials through an air flow pulverizer, control the particle size to be less than or equal to 80 mesh, and finally use a vibrating screen with a 100-mesh sieve to screen out impurities to ensure the fluidity of the finished powder;

[0040] S4. Transport the mixed materials to a fluidized bed coater, adopt the microencapsulation coating process, with an inlet air temperature of 60°C and the material temperature less than or equal to 40°C. The survival rate of probiotics after coating is greater than or equal to 90%. Then, perform light-shielding pretreatment on the photosensitive components of tea polyphenols and curcumin, operate in a red light illumination environment, and finally remove foreign objects through a metal detector;

[0041] S5. Use an automatic quantitative packaging machine to fill and seal with nitrogen in an environment with a temperature of 25°C and a humidity less than or equal to 30%, and then sample and detect the key indicators.

[0042] Among them, it should be noted that the key indicators in step S5 include the viable count of probiotics, enzyme activity, and heavy metal residues.

[0043] Example 1. In this Example 1, the high-efficiency growth-promoting pig compound additive includes the following components: Bacillus subtilis: 6 parts; Bacillus licheniformis: 4 parts; fructooligosaccharide: 5 parts; galactooligosaccharide: 4 parts; yeast extract: 10 parts; yucca extract: 3 parts; cellulase: 1.5 parts; acid protease: 1.5 parts; curcumin: 1 part; tea polyphenols: 0.5 part; taurine: 2 parts; zinc glycinate chelate: 3 parts. The preparation process is the same as that of the example, and the high-efficiency growth-promoting pig compound additive is prepared according to steps S1 to S6;

[0044] Example 2: In this Example 2, the amount of Bacillus subtilis is increased to 8 parts, and the other components and preparation process are the same as those in Example 1. The high-efficiency growth-promoting pig compound additive is prepared according to steps S1 to S6;

[0045] Example 3: In this Example 3, the amount of fructooligosaccharide is reduced to 4 parts, and the other components and preparation process are the same as those in Example 1. The high-efficiency growth-promoting pig compound additive is prepared according to steps S1 to S6;

[0046] Example 4: In this Example 4, the amount of galactooligosaccharide is reduced to 3 parts, and the other components and preparation process are the same as those in Example 1. The high-efficiency growth-promoting pig compound additive is prepared according to steps S1 to S6;

[0047] Example 5: In this Example 5, the amount of zinc glycine chelate is reduced to 2 parts, and the other components and preparation process are the same as those in Example 1. The high-efficiency growth-promoting pig compound additive is prepared according to steps S1 to S6, as shown in Table 1:

[0048] Table 1 Components of the high-efficiency growth-promoting pig compound additive in Examples 1 to 5

[0049]

[0050]

[0051] Comparative Example 1: In this Comparative Example 1, the amount of Bacillus subtilis is increased to 10 parts by over-addition, and the other components and preparation process are the same as those in Example 1. The high-efficiency growth-promoting pig compound additive is prepared according to steps S1 to S6;

[0052] Comparative Example 2: In this Comparative Example 2, the amount of Bacillus licheniformis is increased to 7 parts by over-addition, and the other components and preparation process are the same as those in Example 1. The high-efficiency growth-promoting pig compound additive is prepared according to steps S1 to S6;

[0053] Comparative Example 3: In this Comparative Example 3, only 2 parts of fructooligosaccharide are added in a small amount, and the other components and preparation process are the same as those in Example 1. The high-efficiency growth-promoting pig compound additive is prepared according to steps S1 to S6;

[0054] Comparative Example 4: In this Comparative Example 4, only 1 part of galactooligosaccharide is added in a small amount, and the other components and preparation process are the same as those in Example 1. The high-efficiency growth-promoting pig compound additive is prepared according to steps S1 to S6;

[0055] Comparative Example 5: In this Comparative Example 5, the amount of zinc glycine chelate is increased to 6 parts by over-addition, and the other components and preparation process are the same as those in Example 1. The high-efficiency growth-promoting pig compound additive is prepared according to steps S1 to S6, as shown in Table 2:

[0056] Table 2 Components of the High-efficiency Growth-promoting Compound Additive for Pigs in Comparative Examples 1 to 5

[0057]

[0058]

[0059] III. Performance Detection:

[0060] The usage method of the high-efficiency growth-promoting compound additive for pigs is as follows: It is mixed into the basal diet at a ratio of 0.5 - 1.2%, and is applicable to growing and finishing pigs weighing 15 - 90 kg. Avoid using it in combination with strong oxidizing additives or broad-spectrum antibiotics to prevent the destruction of the activity of probiotics. The probiotics and enzyme preparations are processed by the microcapsule coating process to ensure that the survival rate after high-temperature granulation is greater than 80%. The detection standards are as follows:

[0061] a. Growth-promoting effect: Average daily gain (ADG, g / day) for 30 days. The initial weight of the experimental pigs is 15 kg, and they are fed ad libitum;

[0062] b. Intestinal flora stability: Collect fecal samples and determine the ratio of Bifidobacterium to Escherichia coli (qPCR method);

[0063] c. Photosensitizing toxicity: Simulate outdoor light (ultraviolet intensity 50 μW / cm 2 ), and record the incidence of skin erythema (%);

[0064] d. Retention rate of enzyme activity: Cellulase activity (CMC method, U / g), acid protease activity (casein method, U / g);

[0065] e. Heavy metal residues: Determine the zinc content (mg / kg) by atomic absorption spectrometry, collate and collect the data, as shown in Table 3 and Table 4:

[0066] Table 3 Performance Data of Examples

[0067]

[0068] Table 4 Performance Data of Comparative Examples

[0069]

[0070]

[0071] IV. Analysis Conclusion:

[0072] As can be seen from Table 3 and Table 4, in Example 1 of the present invention (6 parts of Bacillus subtilis + 5 parts of fructooligosaccharide), the flora ratio reaches 8.2, which is significantly higher than that in Comparative Example 3 (the ratio is 3.8 when fructooligosaccharide is insufficient), indicating the synergistic effect of prebiotics and probiotics;

[0073] Example 1 had a zinc residue of 120 mg / kg (the safety limit is less than or equal to 150 mg / kg), while Comparative Example 5 (with 6 parts of excessive zinc) had a residue of 210 mg / kg, presenting a risk of exceeding the standard.

[0074] In Example 1, the incidence of erythema was 2%, while in Comparative Example 5 (with excessive tea polyphenols) it was 35%, indicating a reasonable ratio of photosensitive components within the formulation range.

[0075] In summary, the ratio of 6 parts of Bacillus subtilis to 5 parts of fructooligosaccharide in Example 1 resulted in an ADG of 780 g / day, with the optimal ratio of the microbial population.

[0076] Both the zinc residue and the incidence of erythema were lower than the safety thresholds, and the enzyme activity retention rates (4800 U / g for cellulase and 8200 U / g for acid protease) were 20 - 30% higher than those in Comparative Example 2.

[0077] Within the formulation range of the present invention, Example 1 achieved the best balance of efficient growth promotion, intestinal health maintenance, and safety through precise formulation. In contrast, the performance of the comparative examples decreased significantly due to excess or deficiency, verifying the scientific nature of the formulation range design. In summary, Example 1 of the present invention has the optimal comprehensive performance and is suitable for large-scale pig feed production.

[0078] In the description of this specification, the descriptions referring to terms such as "one embodiment", "example", "specific example", etc. mean that the specific features, structures, materials, or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials, or characteristics described can be combined in a suitable manner in any one or more embodiments or examples.

[0079] The preferred embodiments of the present invention disclosed above are only used to help illustrate the present invention. The preferred embodiments do not elaborate on all the details, nor do they limit the invention to the specific embodiments described. Obviously, many modifications and variations can be made according to the content of this specification. These embodiments are selected and specifically described in this specification to better explain the principles and practical applications of the present invention, so that those skilled in the art can understand and utilize the present invention well. The present invention is only limited by the claims and their full scope and equivalents.

Claims

1. A highly efficient growth-promoting pig composite additive, characterized in that: The invention is composed of the following raw materials in parts by weight: 5-8 parts of Bacillus subtilis, 3-5 parts of Bacillus licheniformis, 4-6 parts of fructooligosaccharide, 3-5 parts of galacto-oligosaccharide, 8-12 parts of yeast extract, 2-4 parts of yucca extract, 1-2 parts of cellulase, 1-2 parts of acid protease, 0.5-1.5 parts of curcumin, 0.3-0.8 parts of tea polyphenols, 1-3 parts of taurine and 2-4 parts of glycine chelated zinc.

2. The highly efficient growth-promoting composite additive for pigs according to claim 1, characterized in that: The Bacillus subtilis is prepared by liquid fermentation, centrifugal concentration and spray drying of the Bacillus subtilis strain at 30-37°C and pH 6.5-7.2, and the purity is that the number of viable bacteria is greater than or equal to 1×10^10 CFU / g.

3. The highly efficient growth-promoting pig composite additive according to claim 2, characterized in that: The Bacillus licheniformis is prepared by deep fermentation, centrifugal separation and freeze drying of the Bacillus licheniformis strain at 28-35°C and pH 6.8-7.5, and the purity is that the number of viable bacteria is greater than or equal to 5×10^9 CFU / g.

4. The highly effective growth-promoting composite additive for pigs according to claim 3, characterized in that: The yeast extract is prepared by subjecting brewer's yeast to autolysis, centrifugal concentration and spray drying at 45-55° C. and pH 5.0-6.

0.

5. The highly effective growth-promoting composite additive for pigs according to claim 4, characterized in that: The yucca extract is prepared by subjecting the yucca stem to reflux extraction, resin purification and drying at 60-70° C. and 60-70% ethanol.

6. The highly effective growth-promoting composite additive for pigs according to claim 5, characterized in that: The cellulase is prepared by liquid fermentation of Trichoderma at 28-32 DEG C and pH 4.5-5.5, ultrafiltration concentration and carrier adsorption.

7. The highly effective growth-promoting pig composite additive according to claim 6, characterized in that: The taurine is prepared by esterifying, neutralizing, crystallizing and decolorizing ethanolamine at 80-90° C. and pH 1-2.

8. The highly effective growth-promoting composite additive for pigs according to claim 7, characterized in that: The glycine chelated zinc is prepared by chelating glycine and zinc sulfate at 50-60° C. and pH 6.5-7.5 and spray drying.

9. The method for preparing a highly efficient growth-promoting pig composite additive as claimed in any one of claims 1 to 8, characterized in that: The following steps are involved: S1. Weigh the raw materials according to the formula ratio in a clean room with a humidity of less than or equal to 30% and a temperature of 25°C: 5-8 parts of Bacillus subtilis, 3-5 parts of Bacillus licheniformis, 4-6 parts of oligofructose, 3-5 parts of galacto-oligosaccharide, 8-12 parts of yeast extract, 2-4 parts of yucca extract, 1-2 parts of cellulase, 1-2 parts of acid protease, 0.5-1.5 parts of curcumin, 0.3-0.8 parts of tea polyphenols, 1-3 parts of taurine, and 2-4 parts of glycine chelated zinc; S2. First, Bacillus subtilis, Bacillus licheniformis, oligofructose and oligogalactose are put into a three-dimensional motion mixer, and the speed is set to 10-15 rpm for 15 minutes. The probiotics are wrapped with prebiotics to improve stability. Then, yeast extract, yucca extract, cellulase and acid protease are added to another mixing chamber, and mixed for 10 minutes in an environment below 40°C to avoid inactivation of the enzyme preparation. Finally, curcumin, tea polyphenols, taurine and glycine chelated zinc are premixed; S3. The three groups of premixed materials are put into a high-efficiency double-helix conical mixer, the spindle speed is set to 25-30rpm, the spiral belt speed is 100-120rpm, the mixing time is 40-60 minutes, and the coefficient of variation of the mixing uniformity is less than or equal to 5%. Then, the agglomerated materials are finely powdered by a jet mill to control the particle size to be less than or equal to 80 mesh, and finally, the impurities are sieved out by a vibrating sieving machine with a 80-100 mesh screen to ensure the fluidity of the finished powder; S4. The mixed material is conveyed to a fluidized bed coating machine, and a microencapsulation coating process is adopted, with an inlet air temperature of 50-60°C, a material temperature of less than or equal to 40°C, and a probiotic survival rate of greater than or equal to 90% after coating. Then, the photosensitive components of tea polyphenols and curcumin are treated before packaging in a light-proof manner, and the operation is carried out under a red light illumination environment. Finally, foreign matter is removed by a metal detector; S5. Use an automatic quantitative packaging machine to seal the products with nitrogen at a temperature of 25°C and a humidity of 30% or less, and then take samples to test key indicators.

10. The method for preparing a highly efficient growth-promoting pig composite additive according to claim 9, characterized in that: The key indicators of step S5 include the number of viable probiotics, enzyme activity and heavy metal residues.

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