Compound composition for protecting liver and improving intestinal tract as well as preparation method and application of compound composition

Abstract

The invention provides a compound composition for protecting liver and improving intestinal tract as well as a preparation method and application thereof. The compound composition comprises 10-15 parts of a chicory root extract, 10-15 parts of burdock root synanthrin, 3-5 parts of semen cassiae concentrated powder, 2-4 parts of fructus cannabis powder, 1-2 parts of a lotus leaf extract, 5-8 parts of microencapsulated compound probiotic powder, 8-10 parts of chlorogenic acid, 5-10 parts of instant arabica coffee powder, 2-3 parts of inulin, 5-8 parts of fructo-oligosaccharide, 1-2 parts of xylooligosaccharide and 0.2-0.5 part of microencapsulated taurine. 0.1 to 0.3 part of dipotassium glycyrrhizinate, 0.05 to 0.1 part of microencapsulated curcumin and 0.5 to 1 part of vitamin B family; the preparation method comprises the following steps: S1, respectively preparing the chicory root extract, the arabica coffee instant powder, the microencapsulated compound probiotic powder, the microencapsulated curcumin and the microencapsulated taurine; s2, respectively weighing the raw materials in parts by weight; s3, uniformly mixing the weighed raw materials to obtain a compound composition; and S4, packaging and storing the compound composition. The prepared compound composition has the functions of protecting the liver and improving the intestinal tract.

Description

Technical Field

[0001] The present invention relates to a compound composition for protecting the liver and improving the intestine, its preparation method and application, and belongs to the technical field of functional foods. Background Art

[0002] With the acceleration of the modern people's life rhythm, unhealthy eating habits and irregular lifestyles will have a significant adverse impact on the stomach and liver. At the same time, the improvement of people's health awareness makes more and more people begin to pay attention to health care. Products with the functions of protecting the liver and improving intestinal function are therefore favored and have broad market prospects.

[0003] Traditional liver-protecting products, such as products containing ingredients such as bovine liver peptide, bovine lung peptide, eggshell membrane peptide, yeast peptide, wolfberry peptide and collagen peptide, and products containing extracts of various Chinese herbal medicines such as silybum marianum,hovenia dulcis, pueraria lobata and polyene phosphatidylcholine, although having the effects of nourishing and protecting the liver, do not explicitly mention the improvement of intestinal function.

[0004] Traditional stomach-protecting products, such as products containing ingredients such as α-lactalbumin, inulin, camel milk, osteopontin, and products containing ingredients such as regulating intestinal flora, although having a protective effect on the stomach and intestine, do not comprehensively cover all aspects of liver protection.

[0005] Therefore, the research and development of a compound composition with the functions of protecting the liver and improving intestinal function not only meets the urgent needs of the current market, but also has important commercial value and social significance. Summary of the Invention

[0006] In view of the above existing technical problems, the present invention provides a compound composition for protecting the liver and improving the intestine, its preparation method and application, so as to achieve the effects of both protecting the liver and improving the intestine.

[0007] To achieve the above technical purpose, firstly, the present invention provides a compound composition for protecting the liver and improving the intestine, comprising the following raw materials in parts by weight: 10-15 parts of chicory root extract, 10-15 parts of burdock root inulin, 3-5 parts of cassia seed concentrated powder, 2-4 parts of hemp seed powder, 1-2 parts of lotus leaf extract, 5-8 parts of microencapsulated compound probiotic powder, 8-10 parts of chlorogenic acid, 5-10 parts of Arabica instant coffee powder, 2-3 parts of inulin, 5-8 parts of fructooligosaccharide, 1-2 parts of xylooligosaccharide, 0.2-0.5 part of microencapsulated taurine, 0.1-0.3 part of dipotassium glycyrrhizinate, 0.05-0.1 part of microencapsulated curcumin, and 0.5-1 part of vitamin B group.

[0008] Furthermore, the present invention comprises raw materials in the following parts by weight: 12 parts of chicory root extract, 12 parts of inulin from burdock root, 4 parts of concentrated semen cassiae powder, 3 parts of semen cannabis powder, 1.5 parts of lotus leaf extract, 6 parts of microencapsulated compound probiotic powder, 9 parts of chlorogenic acid, 8 parts of instant arabica coffee powder, 2.5 parts of inulin, 6 parts of fructooligosaccharide, 1.5 parts of xylooligosaccharide, 0.3 part of microencapsulated taurine, 0.2 part of dipotassium glycyrrhizinate, 0.08 part of microencapsulated curcumin, and 0.8 part of vitamin B complex.

[0009] Furthermore, the microencapsulated compound probiotic powder of the present invention comprises raw materials in the following parts by weight: 25 parts of Lactiplantibacillus plantarum, 25 parts of Lactobacillus acidophilus, 20 parts of Bifidobacterium animalis subsp. lactis, 15 parts of Bifidobacterium longum subsp. longum, and 15 parts of Lactobacillus rhamnosus.

[0010] In the above technical solution, each probiotic plays an important role in the human body, and the specific introduction is as follows.

[0011] (1) Lactiplantibacillus plantarum: inhibits the growth of pathogenic bacteria and regulates the intestinal flora; enhances immunity and promotes digestion.

[0012] (2) Lactobacillus acidophilus: decomposes lactose and relieves lactose intolerance; maintains intestinal health and competes with pathogenic bacteria for occupancy.

[0013] (3) Bifidobacterium animalis subsp. lactis: adjusts the intestinal flora, inhibits the growth of putrefactive bacteria; promotes intestinal health and has a long history of safe consumption.

[0014] (4) Bifidobacterium longum subsp. longum: regulates the balance of intestinal flora and improves intestinal function; has high acid and bile tolerance and protects intestinal health.

[0015] (5) Lactobacillus rhamnosus: improves the intestinal microecological balance and enhances immunity; prevents and treats diarrhea and promotes intestinal health.

[0016] Furthermore, the vitamin B complex of the present invention comprises raw materials in the following parts by weight: 25 parts of vitamin B1, 25 parts of vitamin B6, and 50 parts of vitamin B12.

[0017] In the above technical solution, the above various raw materials achieve the effects of protecting the liver and / or improving the intestine through different mechanisms, and the specific introduction is as follows.

[0018] (1) Chicory root extract: a. Improves intestinal function: Chicory root contains a large amount of natural inulin (a prebiotic), which can promote the growth of probiotics, improve the balance of intestinal flora, and relieve constipation or diarrhea; b. Liver protection effect: Its components can reduce liver inflammation, inhibit the NF-KB pathway, and reduce IL-6, and are used to relieve liver problems such as hepatitis and fatty liver.

[0019] (2)Inulin from burdock root: Promotes the growth of probiotics, preferentially utilized by Bifidobacterium and Lactobacillus; inhibits harmful bacteria (such as Escherichia coli, Salmonella), regulates intestinal function; improves constipation and diarrhea, and alleviates irritable bowel syndrome (IBS).

[0020] (3)Concentrated powder of Cassia obtusifolia seeds: a. Improves intestinal function: Chrysophanol (≥5%) contained inhibits cholesterol 7α-hydroxylase (CYP7A1), moistens the intestines and promotes defecation, reduces dry feces in the intestines through "cooling blood", and promotes bowel movements; alleviates functional constipation (especially suitable for those with the constitution of yin deficiency and excessive fire); in combination with Cannabis seed, it has the effect of enhancing intestinal moistening. b. Protects the liver: The bitter principle components contained inhibit cholesterol synthase (ACAT), reducing the deposition of low-density lipoprotein (LDL); chrysophanol contained alleviates alcoholic liver injury and reduces the levels of serum ALT / AST.

[0021] (4)Cannabis seed: Contains linolenic acid, regulates intestinal neurotransmitter (5-HT), alleviates IBS symptoms, and improves intestinal function.

[0022] (5)Extract of lotus leaf: Contains flavonoids that inhibit lipase activity, assists in controlling fat, and has a liver-protecting effect.

[0023] (6)Microencapsulated compound probiotic powder (including Lactiplantibacillus plantarum, Lactobacillus acidophilus, Bifidobacterium animalis subsp. lactis, Bifidobacterium longum subsp. longum, Lactobacillus rhamnosus): a. Directly regulates the intestinal flora and enhances the intestinal mucosal barrier function: By increasing the number of probiotics in the intestine, regulates the balance of the intestinal flora, helps alleviate gastrointestinal problems such as diarrhea and constipation, and targets the regulation of the intestinal mucosal barrier. b. Enhances immunity: Can stimulate the immune system, improve human immunity, and fight against harmful bacteria and viruses. c. Promotes nutrient absorption: Helps promote the absorption and metabolism of nutrients, and improves the absorption efficiency of important nutrients by the human body.

[0024] (7)Chlorogenic acid and inulin: a. Improves intestinal function: Promotes the balance of the intestinal flora. b. Protects the liver: Has antioxidant properties and reduces the burden on the liver.

[0025] (8)Instant Arabica coffee powder: a. Antioxidant effect: Contains phenolic compounds such as chlorogenic acid and caffeic acid, which are beneficial for scavenging free radicals and delaying cellular oxidative damage. b. Assists in blood sugar control: Inhibits the activity of α-glucosidase, and in vitro experiments show that it can reduce the blood sugar rise by 20%-30%. c. Intestinal friendliness: Chlorogenic acid regulates the intestinal flora and may improve the symptoms of irritable bowel syndrome (IBS). d. Refreshing effect: Contains caffeine (0.8-1.4%), which provides a mild refreshing effect.

[0026] (9)Fructooligosaccharides and xylooligosaccharides: They have the function of improving intestinal function, synergizing with chicory root extract to promote the proliferation of probiotics.

[0027] ‌(10)Microencapsulated taurine: It has the function of protecting the liver and enhancing antioxidant capacity.

[0028] (11)Dipotassium glycyrrhizinate: It inhibits hepatocyte apoptosis (downregulating Caspase-3), promotes glutathione synthesis; has anti-inflammatory and liver-protecting effects, inhibits hepatocyte injury factors, and reduces the levels of ALT / AST.

[0029] (12)Microencapsulated curcumin: It has antioxidant, anti-inflammatory effects, supports liver repair, and has a liver-protecting effect; nano-liposome encapsulation improves bioavailability (>80%) and targets the liver Nrf2 pathway.

[0030] (13)B vitamins (B1, B6, B12): They assist the liver in metabolism and detoxification, relieve fatigue, and have a liver-protecting effect.

[0031] ‌Moreover, after the above various raw materials are combined together, the compound composition can not only effectively improve intestinal function but also significantly protect the liver, as analyzed below.

[0032] a. Liver-protecting effect: It mainly reduces liver inflammation, has antioxidant effects, assists the liver in metabolism and detoxification, reduces the levels of serum ALT / AST, and supports liver repair through raw materials such as chicory root extract, chlorogenic acid, concentrated semen cassiae powder, lotus leaf extract, dipotassium glycyrrhizinate, B vitamins, microencapsulated taurine, and microencapsulated curcumin.

[0033] b. Intestinal function improvement: It mainly promotes the growth of probiotics, regulates the balance of intestinal flora, and relieves symptoms such as constipation, diarrhea, and irritable bowel syndrome through raw materials such as chicory root extract, inulin from burdock root, fructooligosaccharides, xylooligosaccharides, chlorogenic acid, concentrated semen cassiae powder, hemp seed, instant arabica coffee powder, and compound probiotic powder.

[0034] ‌In particular, through various synergistic mechanisms, the above various raw materials make the compound composition have significant effects in protecting the liver and improving intestinal function, as analyzed below.

[0035] A. Bidirectional regulation of the liver-intestine axis: The liver-intestine metabolic dialogue is achieved through the inulin-probiotic-bile acid cycle (FGF19 / FGF15 axis). Specifically, prebiotics such as burdock root inulin, chicory root extract, and fructooligosaccharides promote the proliferation of probiotics (such as Lactiplantibacillus plantarum, Lactobacillus acidophilus, etc.), improving the balance of the intestinal flora. Short-chain fatty acids (SCFAs) and secondary bile acids produced by probiotic metabolism feedback-regulate liver metabolism through the FGF19 / FGF15 axis, achieving bidirectional dialogue between the liver and the intestine. Moreover, probiotics regulate bile acid metabolism, reducing the toxic accumulation of bile acids in the liver, while promoting liver cholesterol metabolism and reducing the risk of fatty liver.

[0036] B. Antioxidant network: Chlorogenic acid + microencapsulated curcumin + B vitamins form a synergistic antioxidant system (with a 300% increase in ORAC value). Specifically, chlorogenic acid, microencapsulated curcumin, and B vitamins (B1, B6, B12) form a synergistic antioxidant system, significantly increasing the ORAC value (antioxidant capacity) by 300%. Chlorogenic acid and microencapsulated curcumin scavenge free radicals, reducing oxidative stress-induced damage to hepatocytes, while B vitamins assist liver metabolism and detoxification, enhancing antioxidant capacity. Moreover, phenolic compounds such as chlorogenic acid and caffeic acid assist in blood sugar control by inhibiting α-glucosidase activity, while regulating the intestinal flora and improving the symptoms of irritable bowel syndrome (IBS).

[0037] C. Synergistic effect of inulin and chlorogenic acid: Chicory root extract and inulin reduce fat deposition in the liver and lower the risk of liver inflammation, while the antioxidant and anti-inflammatory effects of chlorogenic acid further strengthen the protection of the liver, preventing damage to hepatocytes and thus more comprehensively maintaining liver health.

[0038] D. Synergistic effect of burdock root inulin and probiotics: Burdock root inulin promotes the massive reproduction of Bifidobacterium. The short-chain fatty acids produced by Bifidobacterium can lower the intestinal pH value, inhibit the growth of harmful bacteria, and at the same time enhance intestinal peristalsis, making feces easier to excrete, thus improving the digestive and excretory functions of the intestine.

[0039] E. Synergistic effect of concentrated semen cassiae powder and semen cannabis powder: Chrysophanol in semen cassiae and linolenic acid in semen cannabis improve intestinal function by laxative and regulating intestinal neurotransmitters, while reducing the serum ALT / AST levels and alleviating alcoholic liver injury.

[0040] F. Synergistic effect of dipotassium glycyrrhizinate and microencapsulated taurine: Dipotassium glycyrrhizinate reduces hepatocyte injury factors through anti-inflammatory and liver-protecting effects, while microencapsulated taurine enhances antioxidant capacity and supports liver repair.

[0041] Second, the present invention provides a method for preparing a compound composition for protecting the liver and improving the intestine, comprising the following steps: S1. Prepare chicory root extract, instant Arabica coffee powder, microencapsulated compound probiotic powder, microencapsulated curcumin, and microencapsulated taurine respectively.

[0042] Further, in the method of the present invention, the preparation of chicory root extract includes the following steps: A1. Select high-quality chicory roots, remove impurities and wash them clean. A2. Perform ultrasonic-assisted extraction of the washed chicory roots in water, with a solid-liquid ratio of 1:10, adjust the pH to 4.5, extract at 40 °C for 30 - 60 min, and filter to obtain chicory root extract. A3. Add anhydrous ethanol to the chicory root extract until the final ethanol concentration reaches 75%, let it stand for 4 h and then filter to obtain chicory root filtrate. A4. Spray-dry the chicory root filtrate to obtain chicory root powder. A5. Crush and sieve the chicory root powder to obtain chicory root extract.

[0043] In the above technical solution, compared with the traditional heat reflux extraction, ultrasonic-assisted extraction can efficiently break the cell wall of chicory roots at 40 °C, promote the dissolution of active ingredients, shorten the extraction time, and improve production efficiency.

[0044] Further, in the method of the present invention, the preparation of instant Arabica coffee powder includes the following steps: B1. Select high-quality Arabica coffee beans, remove defective beans, foreign objects and broken beans. B2. Roast the Arabica coffee beans at 120 - 160 °C for 20 - 30 min to obtain roasted coffee beans. B3. Crush and sieve the roasted coffee beans to obtain roasted coffee powder. B4. Perform supercritical carbon dioxide extraction on the roasted coffee powder, extract at 30 MPa and 40 °C for 2 - 4 h, and filter to obtain coffee extract. B5. Freeze-dry the coffee extract to obtain instant Arabica coffee powder.

[0045] In the above technical solution, low-temperature roasting can reduce the loss of nutrients such as chlorogenic acid in coffee beans. The instant coffee prepared by supercritical carbon dioxide extraction has a richer aroma and a more mellow taste compared with traditional solvent extraction, and the extraction process is relatively mild, which can further protect the thermosensitive components in coffee. Supercritical carbon dioxide extraction can efficiently extract the soluble substances in coffee while avoiding the destruction of coffee flavor by high temperature. Freeze-drying technology can maximize the retention of the original aroma and flavor of coffee.

[0046] Further, in the method of the present invention, the preparation of microencapsulated compound probiotic powder includes the following steps: C1. After culturing each probiotic strain to the logarithmic growth phase, collect the bacterial cells and mix them to obtain a composite probiotic bacterial cell mass; C2. Suspend the composite probiotic bacterial cell mass in a 1.5%-2.0% sodium alginate solution for inner layer embedding, and obtain sodium alginate microcapsules by spray drying or extrusion method; C3. Immerse the sodium alginate microcapsules in a 0.5%-1.0% chitosan solution for outer layer embedding to obtain composite probiotic microcapsules; C4. Conduct a pH 2.0 simulated gastric juice test on the composite probiotic microcapsules to ensure that the survival rate > 85%; C5. Dry and solidify the qualified composite probiotic microcapsules to make composite probiotic powder.

[0047] In the above technical solution, through double-layer embedding and acid resistance test, composite probiotic powder with good acid resistance and high survival rate can be prepared, effectively resisting the damage of gastric acid to probiotics, enabling more live bacteria to reach the intestine smoothly and play their beneficial roles such as regulating intestinal flora and improving intestinal health.

[0048] Furthermore, in the method of the present invention, for the preparation of microencapsulated curcumin with curcumin as the active ingredient and the preparation of microencapsulated taurine with taurine as the active ingredient, the following steps are included: D1. Mix the active ingredient with sodium alginate for inner layer embedding, and form inner layer microcapsules by spray drying or extrusion method; ‌D2. Mix the inner layer microcapsules with chitosan for outer layer embedding to form double-layer microcapsules; ‌D3. Dry and solidify the double-layer microcapsules to obtain microencapsulated active ingredient.

[0049] In the above technical solution, the purpose of inner layer embedding is to protect curcumin or taurine from being rapidly degraded in the gastric environment and achieve a sustained release effect. The purpose of outer layer embedding is to ensure the release of curcumin or taurine in the intestinal environment and achieve a targeted effect. And the stability and mechanical strength are enhanced through microcapsule solidification. Therefore, compared with single-layer microcapsules, the double-layer microcapsule structure further enhances the protection effect on the internal active ingredient and provides guarantee for the stable transmission of curcumin or taurine in the complex gastrointestinal environment.

[0050] S2. Weigh each raw material by weight parts.

[0051] S3. Mix the weighed raw materials evenly to obtain a compound composition.

[0052] S4. Package and store the compound composition.

[0053] Thirdly, the present invention provides an application of the compound composition in the preparation of a functional food, and the functional food has the functions of protecting the liver and improving the intestine.

[0054] In the above technical solution, the functional food has the following functions: a. Liver protection effect: improving alcoholic / non-alcoholic fatty liver and chemical liver injury; b. Intestinal regulation: relieving constipation, diarrhea and IBS; c. Metabolic assistance: regulating blood sugar and blood lipids to achieve overall regulation of "liver-intestine-metabolism".

[0055] In summary, the present invention takes chicory root extract, inulin from burdock root, microencapsulated probiotics, concentrated powder of semen cassiae, hemp seed powder, and lotus leaf extract as the core components, and takes chlorogenic acid, fructooligosaccharide, xylooligosaccharide, inulin, microencapsulated taurine, and instant coffee powder of arabica as auxiliary components. Through various synergistic effects, it can significantly improve liver health and intestinal function, and can also effectively protect the liver, having high market application value. Description of the Drawings

[0056] Figure 1 It is a flow chart of the preparation method of the present invention; Figure 2 It is a statistical chart of the results of Experiment 1 of the present invention. Detailed Embodiments

[0057] The present invention will be further explained below in conjunction with specific embodiments. Those skilled in the art will understand that the following described embodiments are part of the embodiments of the present invention, rather than all of the embodiments, and are only used to illustrate the present invention and should not be regarded as limiting the scope of the present invention. All the reagents used are conventional products that can be obtained through commercial purchase.

[0058] Example 1: This example provides a compound composition for protecting the liver and improving the intestine, including the following raw materials and their weights: 10 parts of chicory root extract, 10 parts of inulin from burdock root, 3 parts of concentrated powder of semen cassiae, 2 parts of hemp seed powder, 1 part of lotus leaf extract, 5 parts of microencapsulated compound probiotic powder, 8 parts of chlorogenic acid, 5 parts of instant coffee powder of arabica, 2 parts of inulin, 5 parts of fructooligosaccharide, 1 part of xylooligosaccharide, 0.2 part of microencapsulated taurine, 0.1 part of dipotassium glycyrrhizinate, 0.05 part of microencapsulated curcumin, and 0.5 part of vitamin B complex.

[0059] Among them, the microencapsulated compound probiotic powder includes the following raw materials in parts by weight: 25 parts of lactiplantibacillus plantarum, 25 parts of lactobacillus acidophilus, 20 parts of bifidobacterium animalis subsp. lactis, 15 parts of bifidobacterium longum subsp. longum, and 15 parts of lactobacillus rhamnosus.

[0060] The vitamin B complex includes the following raw materials in parts by weight: 25 parts of vitamin B1, 25 parts of vitamin B6, and 50 parts of vitamin B12.

[0061] And, as Figure 1 shown, the preparation method of the above compound composition is specifically introduced as follows.

[0062] S1. Prepare chicory root extract, instant Arabica coffee powder, microencapsulated compound probiotic powder, microencapsulated curcumin, and microencapsulated taurine respectively.

[0063] Specifically, when implementing, the preparation of the chicory root extract includes the following steps: ‌A1. Raw material preparation‌: Select high-quality chicory roots, remove impurities and wash them cleanly.

[0064] A2. ‌Ultrasonic-assisted extraction‌: Perform ultrasonic-assisted extraction on the washed chicory roots. Use water as the extraction solvent, with a material-liquid ratio of 1:10, adjust the pH value to 4.5, the extraction temperature is 40 °C, and the extraction time is 30 - 60 min. After filtration, obtain the chicory root extract. And ultrasonic extraction helps to improve the extraction efficiency and ensure the full release of active ingredients.

[0065] A3. ‌Alcohol precipitation and purification‌: Slowly add absolute ethanol to the chicory root extract until the final concentration of ethanol reaches 75%, then let it stand for 4 h and filter again to obtain the chicory root filtrate.

[0066] A4.‌‌ Spray drying‌: Perform spray drying on the chicory root filtrate, control the inlet air temperature at 180 °C, and the pressure is 0.1 - 0.3 MPa to obtain the chicory root powder. During the spray drying process, the uniformity and quality of the powder can be ensured by adjusting the inlet air temperature, outlet air temperature, air supply volume, and air extraction volume.

[0067] A5.‌‌‌ Crushing and sieving‌: Crush and refine the chicory root powder through a sieve to remove lumpy connections and ensure that the powder mesh number meets the requirements.

[0068] Specifically, when implementing, the preparation of the instant Arabica coffee powder includes the following steps: B1. Select high-quality Arabica coffee beans and remove defective beans, foreign objects, and broken beans.

[0069] B2. Low-temperature roasting‌: Roast the Arabica coffee beans at 120 - 160 °C for 20 - 30 minutes to obtain roasted coffee beans. And low-temperature roasting helps to retain the aroma and flavor of the coffee beans while reducing the generation of harmful substances.

[0070] B3. Crushing: Crush and sieve the roasted coffee beans to obtain roasted coffee powder.

[0071] B4. Supercritical CO2 extraction: Using supercritical CO2 extraction technology, roasted coffee powder is extracted at a pressure of 30MPa, the extraction temperature is 40℃, the extraction time is 2-4h, and then filtered through a mesh to obtain coffee extract. This method can efficiently extract soluble substances in coffee while avoiding the damage of high temperature to the coffee flavor.

[0072] B5. Freeze drying: Freeze the coffee extract at -40 degrees Celsius, then heat it in a vacuum state to make the water sublime directly, and finally make Arabica instant coffee powder. In addition, freeze drying technology can retain the original aroma and flavor of coffee to the greatest extent.

[0073] In specific implementation, the preparation of microencapsulated composite probiotic powder comprises the following steps: C1. Probiotic culture and collection: The strains of Lactobacillus plantarum, Lactobacillus acidophilus, Bifidobacterium animalis lactis, Bifidobacterium longum subsp. longum, and Lactobacillus rhamnosus are cultured under appropriate conditions, and after culturing to the logarithmic growth phase, the cells of Lactobacillus plantarum, Lactobacillus acidophilus, Bifidobacterium animalis lactis, Bifidobacterium longum subsp. longum, and Lactobacillus rhamnosus are collected by centrifugation or filtration, and then mixed to obtain composite probiotic cells, and the composite probiotic cells are washed with sterile saline to remove culture medium residues, so as to facilitate subsequent sodium alginate-chitosan double-layer embedding.

[0074] C2. Inner layer encapsulation: The collected composite probiotic bacteria are suspended in a 1.5%-2.0% sodium alginate solution, stirred evenly, and then formed into sodium alginate microcapsules with a particle size of 50-80 μm by spray drying or extrusion.

[0075] ‌C3, outer layer embedding‌: Sodium alginate microcapsules are immersed in 0.5%-1.0% chitosan solution and allowed to stand for 10-15 minutes to allow chitosan to form a protective layer on the surface of the microcapsules to obtain composite probiotic microcapsules. The excess chitosan is then washed with sterile water to remove the excess chitosan‌.

[0076] C4. Acid resistance test: Place the embedded composite probiotic microcapsules in simulated gastric fluid at pH 2.0 and incubate at 37°C for 2 hours; determine the survival rate by plate counting method to ensure that the survival rate is >85%.

[0077] ‌C5. Drying‌: freeze-dry or spray-dry the composite probiotic microcapsules that have passed the acid resistance test to prepare composite probiotic powder.

[0078] In specific implementation, the preparation of microencapsulated curcumin comprises the following steps: D1-1, Inner embedding (stomach sustained-release layer): Select curcumin as the core active ingredient to ensure its purity and activity. Mix curcumin with stomach sustained-release materials (such as sodium alginate), and form curcumin microcapsules by spray drying or extrusion method. The purpose of inner embedding is to protect curcumin from being rapidly degraded in the stomach environment and achieve the sustained-release effect.

[0079] D2-2, Outer embedding (intestinal-targeted release layer): Mix curcumin with intestinal-targeted materials (such as chitosan), and wrap it outside the inner microcapsules to form a double-layer structure. The purpose of outer embedding is to ensure the release of curcumin in the intestinal environment and achieve the targeted effect.

[0080] D3-3, Microcapsule solidification: Carry out solidification treatment on the double-layer embedded microcapsules, such as freeze drying or spray drying, to enhance their stability and mechanical strength.

[0081] Specifically in implementation, the preparation of microencapsulated taurine includes the following steps: D1-a, Inner embedding: Select taurine as the core active ingredient. Mix taurine with sodium alginate, and form taurine microcapsules by spray drying or extrusion method.

[0082] D2-b, Outer embedding: Mix the taurine microcapsules with chitosan, and wrap it outside the inner taurine microcapsules to form a double-layer structure.

[0083] D3-c, Microcapsule solidification: Carry out freeze drying or spray drying on the double-layer embedded taurine microcapsules to make microencapsulated taurine.

[0084] S2, Weigh raw materials: Weigh chicory root extract, inulin from burdock root, instant arabica coffee powder, concentrated semen cassiae powder, semen cannabis powder, lotus leaf extract, microencapsulated compound probiotic powder, chlorogenic acid, inulin, fructooligosaccharide, xylooligosaccharide, microencapsulated taurine, dipotassium glycyrrhizinate, microencapsulated curcumin, and vitamin B complex respectively.

[0085] S3, Stir and mix: Mix the weighed raw materials and stir evenly to obtain a compound composition.

[0086] S4, Package and store: Package the compound composition and store it in a dry room.

[0087] Example 2: This example provides a compound composition for protecting the liver and improving the intestine. The difference from Example 1 is that it includes the following raw materials and their weights: 15 parts of chicory root extract, 15 parts of inulin from burdock root, 5 parts of concentrated semen cassiae powder, 4 parts of semen cannabis, 2 parts of lotus leaf extract, 8 parts of microencapsulated compound probiotic powder, 10 parts of chlorogenic acid, 10 parts of instant Arabica coffee powder, 3 parts of inulin, 8 parts of fructooligosaccharide, 2 parts of xylooligosaccharide, 0.5 part of microencapsulated taurine, 0.3 part of dipotassium glycyrrhizinate, 0.1 part of microencapsulated curcumin, and 1 part of vitamin B complex. And the preparation method of the above compound composition is the same as that of Example 1.

[0088] Example 3: This example provides a compound composition for protecting the liver and improving the intestine. The difference from Example 1 is that it includes the following raw materials in parts by weight: 12 parts of chicory root extract, 12 parts of inulin from burdock root, 4 parts of concentrated semen cassiae powder, 3 parts of semen cannabis powder, 1.5 parts of lotus leaf extract, 6 parts of microencapsulated compound probiotic powder, 9 parts of chlorogenic acid, 8 parts of instant Arabica coffee powder, 2.5 parts of inulin, 6 parts of fructooligosaccharide, 1.5 parts of xylooligosaccharide, 0.3 part of microencapsulated taurine, 0.2 part of dipotassium glycyrrhizinate, 0.08 part of microencapsulated curcumin, and 0.8 part of vitamin B complex. And the preparation method of the above compound composition is the same as that of Example 1.

[0089] Comparative Example 1: This comparative example provides a compound composition. The difference from Example 3 is that it lacks chicory root extract. And the preparation method of the above compound composition is the same as that of Example 1, only lacking the step of preparing chicory root extract.

[0090] Comparative Example 2: This comparative example provides a compound composition. The difference from Example 3 is that it lacks inulin from burdock root. And the preparation method of the above compound composition is the same as that of Example 1, only lacking the step of weighing inulin from burdock root.

[0091] Comparative Example 3: This comparative example provides a compound composition. The difference from Example 3 is that it lacks concentrated semen cassiae powder. And the preparation method of the above compound composition is the same as that of Example 1, only lacking the step of weighing concentrated semen cassiae powder.

[0092] Comparative Example 4: This comparative example provides a compound composition. The difference from Example 3 is that it lacks semen cannabis powder. And the preparation method of the above compound composition is the same as that of Example 1, only lacking the step of weighing semen cannabis powder.

[0093] Comparative Example 5: This comparative example provides a compound composition. The difference from Example 3 is that it lacks lotus leaf extract. And the preparation method of the above compound composition is the same as that of Example 1, only lacking the step of weighing lotus leaf extract.

[0094] Comparative Example 6: This comparative example provides a compound composition, which is different from Example 3 in that it lacks the microencapsulated compound probiotic powder. Moreover, the preparation method of the above compound composition is the same as that of Example 1, only lacking the step of preparing the microencapsulated compound probiotic powder.

[0095] Comparative Example 7: This comparative example provides a compound composition, which is different from Example 3 in that it lacks chlorogenic acid. Moreover, the preparation method of the above compound composition is the same as that of Example 1, only lacking the step of weighing chlorogenic acid.

[0096] Experiment 1: Liver protection function experiment.

[0097] Experimental subjects and grouping: 120 male Kunming mice with a body weight of 18 ± 2 g were selected. After 7 days of adaptive feeding, the mice were randomly divided into 12 groups with 10 mice in each group, namely: normal control group, model control group, Example 1-3 groups, Comparative Example 1-7 groups. And except for the normal control group, the other groups needed to be modeled first.

[0098] Experimental method: The Example 1-3 groups and the Comparative Example 1-7 groups were respectively given the compound compositions prepared in the above Examples 1-3 and Comparative Examples 1-7 (2 g / kg body weight). The blank control group and the model control group were both given the same dose of distilled water. All mice were fed with a complete nutrition formulated feed and allowed to eat and drink freely. They were continuously gavaged for 30 days.

[0099] Then, on the 30th day, the mice in each group were fasted overnight for 16 h. Then, the model control group, the Example 1-3 groups, and the Comparative Example 1-7 groups were all gavaged once with a 1% CCl 4 vegetable oil solution (5 mL / kg·BW), and the blank control group was given an equal amount of vegetable oil.

[0100] Next, the Example 1-3 groups and the Comparative Example 1-7 groups continued to be respectively given the compound compositions prepared in the above Examples 1-3 and Comparative Examples 1-7 until the end of the experiment (with a 4 h interval between gavages and CCl 4 gavage). After 24 h, the mice were sacrificed by eye enucleation to collect blood, and the serum ALT and AST levels were measured. 4 24 h later, the mice were sacrificed by eye enucleation to collect blood, and the serum ALT and AST levels were measured.

[0101] It should be noted that measuring the levels of serum ALT (alanine aminotransferase) and AST (aspartate aminotransferase) is an important indicator for evaluating liver function and can reflect the liver protection effect to a certain extent. Among them, ALT is mainly present in the cytoplasm of hepatocytes. When hepatocytes are damaged, ALT will be released into the blood, and the level of ALT in the blood increases, which is a sensitive indicator of hepatocyte damage. AST is present in the mitochondria and cytoplasm of hepatocytes. When hepatocytes are severely damaged or necrotic, AST will also be released into the blood. If ALT and AST continue to rise, it indicates that the condition may worsen; if they gradually decline, it indicates that the hepatocyte damage has decreased, the condition is improving, and the liver protection treatment is effective.

[0102] The experimental results are shown in Table 1 below.

[0103]

[0104] From Table 1 and Figure 2 it can be seen that: compared with the blank group, the levels of ALT and AST in the model control group were significantly increased, indicating that the modeling was successful in inducing obvious liver damage.

[0105] The levels of ALT and AST in the Example 3 group were closest to those in the blank group, indicating that the compound composition prepared in Example 3 had the best effect. It not only had a strong ability to repair liver damage, but also could effectively inhibit the abnormal release of enzymes in hepatocytes and reduce liver inflammatory reactions.

[0106] The levels of ALT and AST in the Comparative Example 1-7 groups were higher than those in the Example 1-3 groups, indicating that in the formula of the composition of the present invention, with chicory root extract as the core ingredient and inulin from burdock root, microencapsulated probiotics, concentrated powder of cassia seed, hemp seed powder, and lotus leaf extract as synergistic ingredients, without any of the above raw materials, good liver protection effects could not be achieved. However, when the above raw materials were combined together and the dosage was adjusted, a synergistic liver protection effect could be achieved.

[0107] Specifically, microencapsulated probiotics regulate bile acid metabolism, reduce the toxic accumulation of bile acids in the liver, and at the same time promote liver cholesterol metabolism and reduce the risk of fatty liver. Moreover, inulin from burdock root promotes the growth of probiotics, inhibits harmful bacteria (such as Escherichia coli and Salmonella), regulates intestinal function, improves constipation and diarrhea, and relieves irritable bowel syndrome (IBS). At the same time, the lotus leaf extract contains flavonoids that inhibit lipase activity, assist in controlling fat, and further enhance the liver protection effect. Furthermore, chrysophanol in cassia seed and linolenic acid in hemp seed improve intestinal function by promoting defecation and regulating intestinal neurotransmitters.

[0108] Experiment 2: Experiment on improving intestinal function.

[0109] Experimental subjects and grouping: 110 male Kunming mice with a body weight of 18 ± 2 g were selected. After 7 days of adaptive feeding, the mice were randomly divided into 11 groups, with 10 mice in each group, namely: normal control group, Example 1 - 3 groups, and Comparative Example 1 - 7 groups.

[0110] Experimental method: Before administering the test substances to each group, 0.1 g of feces was aseptically collected from each mouse, and according to the method of examining intestinal flora (Bifidobacterium, Lactobacillus, Enterobacter, Enterococcus) in "Regulating Intestinal Flora Function" of "Technical Specifications for Health Food Inspection and Evaluation" (2003 edition), appropriate dilution degrees were selected and inoculated on the culture medium respectively. After cultivation, colony identification and counting were carried out by colony morphology, Gram staining microscopy, biochemical reactions, etc., and the number of bacteria per gram of wet feces was calculated as the colony situation before administering the test sample.

[0111] Then, the Example 1 - 3 groups and the Comparative Example 1 - 7 groups were respectively given the compound compositions prepared in the above Examples 1 - 3 and Comparative Examples 1 - 7 (2 g / kg body weight) as test substances, and the normal control group was given the same dose of distilled water as the test substance. Each group was continuously intragastrically administered the test substance for 14 days, and 24 hours after the last administration, 0.1 g of feces was aseptically collected from each mouse again, and the intestinal flora was detected by the same method. The changes in Bifidobacterium, Lactobacillus, Enterococcus, and Enterobacter in the feces were compared, with the unit of (log10 cfu / g).

[0112] The experimental results are shown in Table 2 below.

[0113]

[0114] As can be seen from Table 2 above: Compared with the normal control group, the numbers of Bifidobacterium and Lactobacillus in each example group and comparative example group increased to varying degrees after intervention, and the numbers of Enterococcus and Enterobacter mostly decreased.

[0115] Moreover, the effects of the Example 1 - 3 groups were generally better than those of the Comparative Example 1 - 7 groups, indicating that: the compound compositions prepared in Examples 1 - 3 can increase the number of beneficial bacteria and reduce the number of harmful bacteria, playing a role in improving intestinal function. And Examples 1 - 3 use chicory root extract as the core ingredient, and inulin from burdock root, microencapsulated probiotics, concentrated powder of semen cassiae, powder of fructus cannabis, and lotus leaf extract as synergistic ingredients. Without any one of the above raw materials, a good effect on improving intestinal function cannot be achieved. However, when the above raw materials are combined together and the dosage is adjusted, a synergistic effect on improving intestinal function can be achieved.

[0116] Specifically, inulin from burdock root, chicory root extract, fructooligosaccharide, etc. act as prebiotics to promote the proliferation of probiotics and improve the balance of the intestinal flora. In particular, inulin from burdock root promotes the massive reproduction of Bifidobacterium, inhibits the growth of harmful bacteria, and at the same time enhances intestinal peristalsis, making it easier for feces to be excreted from the body, thereby improving the digestive and excretory functions of the intestine. At the same time, short-chain fatty acids (SCFAs) and secondary bile acids produced by the metabolism of microencapsulated probiotics feedback-regulate liver metabolism through the FGF19 / FGF15 axis, realizing the two-way communication between the liver and the intestine. Moreover, chrysophanol in cassia seed and linolenic acid in hemp seed reduce the serum ALT / AST levels and alleviate alcoholic liver injury.

[0117] In addition, combining Experiment 1 and Experiment 2, it can be seen that the compound compositions prepared in Examples 1-3 indeed have the effect of strengthening the spleen and benefiting the stomach, and thus can be applied to the preparation of functional foods with liver protection and intestinal improvement.

[0118] Finally, it should be noted that the examples are only the optimal specific implementation manners of the present invention and are not used to limit the present invention. Although the present invention has been described in detail with reference to the foregoing examples, for those skilled in the art, they can still modify the technical solutions described in the foregoing examples, or perform equivalent replacements on some of the technical features. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. A composite composition for protecting the liver and improving the intestine, characterized in that: The invention comprises the following raw materials in parts by weight: 10-15 parts of chicory root extract, 10-15 parts of burdock root inulin, 3-5 parts of cassia seed concentrated powder, 2-4 parts of hemp seed powder, 1-2 parts of lotus leaf extract, 5-8 parts of microencapsulated composite probiotic powder, 8-10 parts of chlorogenic acid, 5-10 parts of Arabica instant coffee powder, 2-3 parts of inulin, 5-8 parts of oligofructose, 1-2 parts of oligoxylose, 0.2-0.5 parts of microencapsulated taurine, 0.1-0.3 parts of dipotassium glycyrrhizinate, 0.05-0.1 parts of microencapsulated curcumin and 0.5-1 parts of B vitamins.

2. The composite composition for protecting the liver and improving the intestine according to claim 1, characterized in that: The invention comprises the following raw materials in parts by weight: 12 parts of chicory root extract, 12 parts of burdock root inulin, 4 parts of cassia seed concentrated powder, 3 parts of hemp seed powder, 1.5 parts of lotus leaf extract, 6 parts of microencapsulated composite probiotic powder, 9 parts of chlorogenic acid, 8 parts of Arabica instant coffee powder, 2.5 parts of inulin, 6 parts of oligofructose, 1.5 parts of oligoxylose, 0.3 parts of microencapsulated taurine, 0.2 parts of dipotassium glycyrrhizinate, 0.08 parts of microencapsulated curcumin and 0.8 parts of B vitamins.

3. The composite composition for protecting the liver and improving the intestine according to claim 1 or 2, characterized in that: The microencapsulated composite probiotic powder comprises the following raw materials in parts by weight: 25 parts of Lactobacillus plantarum, 25 parts of Lactobacillus acidophilus, 20 parts of Bifidobacterium animalis subsp. lactis, 15 parts of Bifidobacterium longum subsp. longum, and 15 parts of Lactobacillus rhamnosus.

4. The composite composition for protecting liver and improving intestine according to claim 1 or 2, characterized in that: The vitamin B complex comprises the following raw materials in parts by weight: 25 parts of vitamin B1, 25 parts of vitamin B6, and 50 parts of vitamin B12.

5. A method for preparing the composite composition for protecting the liver and improving the intestine according to any one of claims 1 to 4, characterized in that: The steps include: S1, preparing chicory root extract, Arabica instant coffee powder, microencapsulated composite probiotic powder, microencapsulated curcumin, and microencapsulated taurine respectively; S2, weigh each raw material by weight; S3, mixing the weighed raw materials uniformly to obtain a composite composition; S4, packaging and preserving the compound composition.

6. The method for preparing the composite composition for protecting liver and improving intestine according to claim 5, characterized in that: The preparation of chicory root extract comprises the following steps: A1. Choose high-quality chicory roots, remove impurities and wash them clean; A2, subjecting the washed chicory roots to ultrasonic-assisted extraction in water with a solid-liquid ratio of 1:10, adjusting the pH to 4.5, extracting at 40° C. for 30-60 min, and filtering to obtain a chicory root extract; A3, adding anhydrous ethanol to the chicory root extract until the final ethanol concentration reaches 75%, letting it stand for 4 hours and then filtering to obtain a chicory root filtrate; A4, spray drying the chicory root filtrate to obtain chicory root powder; A5. Grind and sieve the chicory root powder to obtain the chicory root extract.

7. The method for preparing the composite composition for protecting liver and improving intestine according to claim 5, characterized in that: The method for preparing Arabica instant coffee powder comprises the following steps: B1. Select high-quality Arabica coffee beans and remove defective beans, foreign matter and broken beans; B2, roasting Arabica coffee beans at 120-160° C. for 20-30 min to obtain roasted coffee beans; B3, crushing and sieving the roasted coffee beans to obtain roasted coffee powder; B4. Extract the roasted coffee powder with supercritical carbon dioxide at 30 MPa and 40°C for 2-4 hours, and obtain coffee extract after filtering; B5. Freeze-drying the coffee extract to obtain Arabica instant coffee powder.

8. The method for preparing the composite composition for protecting liver and improving intestine according to claim 5, characterized in that: The method for preparing the microencapsulated composite probiotic powder comprises the following steps: C1. After culturing each probiotic strain to the logarithmic growth phase, the cells are collected and mixed to obtain composite probiotic cells; C2, suspending the composite probiotic bacteria in a 1.5%-2.0% sodium alginate solution for inner layer embedding, and obtaining sodium alginate microcapsules by spray drying or extrusion; C3, immersing the sodium alginate microcapsules in a 0.5%-1.0% chitosan solution for outer layer embedding to obtain composite probiotic microcapsules; C4. Test the composite probiotic microcapsules with pH 2.0 simulated gastric fluid to ensure that the survival rate is > 85%; C5. Drying and solidifying the qualified composite probiotic microcapsules to prepare composite probiotic powder.

9. The method for preparing the composite composition for protecting liver and improving intestine according to claim 5, characterized in that: The method for preparing microencapsulated curcumin, with curcumin as the active ingredient, and the method for preparing microencapsulated taurine, with taurine as the active ingredient, both comprise the following steps: D1. Mix the active ingredient with sodium alginate for inner layer embedding, and form inner layer microcapsules by spray drying or extrusion; D2. Mix the inner layer microcapsules with chitosan for outer layer embedding to form double-layer microcapsules; D3. Dry and solidify the double-layer microcapsules to obtain microencapsulated active ingredients.

10. Use of the composite composition according to any one of claims 1 to 4 in preparing functional food, characterized in that: The functional food has the functions of protecting the liver and improving the intestinal tract.

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