An emotional beauty composition for relieving stress and reducing skin sensitivity, as well as its preparation method and application

Abstract

The present invention discloses a stress-relieving and skin-sensitive mood-beautifying composition, its preparation method, and application, belonging to the field of cosmetics technology. The mood-beautifying composition provided by the present invention comprises the following components in parts by weight: 0.1-5 parts of Trifolium pratense extract, 0.01-1 parts of Copaiba balsamina resin, 0.1-5 parts of 4-tert-butyl cyclohexanol, and 0.01-2 parts of linseed extract. By selecting the appropriate parts by weight, the present invention achieves a good synergistic effect between the components, can act from both physiological and emotional perspectives, effectively reduce skin sensitivity, improve sensitive symptoms such as skin redness, and enhance the skin's barrier function. The mood-beautifying composition also has excellent anti-aging properties.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and in particular relates to an emotional beauty composition for relieving stress and reducing skin sensitivity, as well as a preparation method and application thereof. Background Art

[0002] Stress has a complex and profound impact on skin health, particularly in terms of increased skin sensitivity. During stressful situations, emotions and stress activate the hypothalamic-pituitary-adrenal (HPA) axis, triggering the release of adrenocorticotropic hormone (CRH) from the hypothalamus, which in turn stimulates the pituitary gland to secrete adrenocorticotropic hormone (ACTH), ultimately promoting the secretion of cortisol by the adrenal glands. Chronic high levels of cortisol can disrupt the skin's barrier function, increase water loss, and make the skin susceptible to irritation. Furthermore, high levels of cortisol can activate neurogenic inflammatory mechanisms. High expression of TRPV1 receptors is closely linked to pain transmission, and stress-induced cortisol release can lead to TRPV1 activation, significantly increasing the skin's sensitivity to external stimuli. Furthermore, increased secretion of substance P during stress activates NK-1 receptors, further exacerbating skin inflammation. Simultaneously, an imbalance in the endocannabinoid system, particularly decreased function of CB1 and CB2 receptors, destabilizes the regulation of pain and inflammation. Therefore, by activating the HPA axis and related receptors, stress damages the skin barrier and triggers neurogenic inflammation, thereby exacerbating skin sensitivity.

[0003] Many skin care products currently on the market target stress-induced skin sensitivity, focusing on interventions through skin sensory receptors such as TRPV1 (skin thermo-pain receptor). These products typically rely on directly acting on these receptors to relieve skin discomfort, such as stinging, burning, and redness. Although such products can alleviate actual skin discomfort to a certain extent, they are often limited to the physiological level and lack the ability to comprehensively regulate the emotional and neurophysiological levels; therefore, their effectiveness is limited. As more and more research deepens, scientists have gradually come to realize the profound impact of emotional states and psychological stress on skin health. Relying solely on inhibitors of physical stimulation cannot fully solve skin health problems caused by stress, especially under long-term or chronic stress, when the skin's sensitive reactions may become more complex. Summary of the Invention

[0004] The purpose of the present invention is to overcome the shortcomings of the above-mentioned existing technologies and provide an emotional beauty composition, as well as a preparation method and application thereof, which can comprehensively act from both physiological and emotional levels, effectively reduce skin sensitivity, improve sensitive symptoms such as skin redness, and at the same time enhance the skin's barrier capacity and have good anti-aging ability.

[0005] To achieve the above objectives, in a first aspect of the present invention, the present invention provides an emotional beauty composition for relieving stress and reducing skin sensitivity, wherein the emotional beauty composition comprises the following components in parts by weight:

[0006] 0.1-5 parts of Trifolium repens extract, 0.01-1 parts of Copaiba resin, 0.1-5 parts of 4-tert-butyl cyclohexanol, and 0.01-2 parts of linseed extract.

[0007] By selecting components in appropriate amounts by weight, the present invention has a good synergistic effect between the components, can act from both physiological and emotional aspects, effectively reduce skin sensitivity, improve sensitive symptoms such as skin redness, and enhance the skin's barrier capacity. At the same time, the emotional beauty composition also has excellent anti-aging ability.

[0008] Specifically, the emotional beauty composition provided by the present invention can act on the brain-skin axis to biphasically regulate and relieve stress and reduce sensitivity. Emotional relaxation not only reduces the negative impact of stress on the skin, but also promotes overall health and well-being. Specifically, in terms of the brain axis, red clover extract can effectively trigger the reaction of the olfactory-limbic system, inhibit the stress response of the amygdala by activating olfactory receptors, and reduce the secretion of cortisol. At the same time, copaiba resin can enhance the activity of the endogenous cannabinoid system, thereby inhibiting the excessive discharge of peripheral nerve endings to cause stimulation. In terms of the skin axis, 4-tert-butyl cyclohexanol is used as a TRPV1 inhibitor, which can directly inhibit the excessive activation of TRPV1 channels, thereby reducing the tingling and discomfort caused by neuroinflammation, and effectively alleviating the inflammatory response caused by skin sensitivity. In addition, flaxseed extract has an antagonistic effect on NK-1 receptors, which can prevent vasodilation and mast cell degranulation caused by substance P, thereby reducing skin redness and sensitivity. Targeted intervention of these facial targets provides direct protection to the skin, improves mood, and helps improve a damaged barrier and facial redness, especially in people with sensitive skin.

[0009] Specifically, red clover is rich in isoflavones, such as genistein, biochanin A, and formononetin. These isoflavones can activate the olfactory-limbic system pathway through nasal olfactory receptors (such as OR1A2), inhibiting the excessive stress response of the amygdala and reducing the secretion of corticotropin-releasing hormone (CRH) in the hypothalamus, thereby reducing cortisol synthesis. The main active ingredients in copaiba resin are terpenoids (α-cedarene, δ-cadinene) and natural cannabinoid analogs (β-caryophyllene). β-caryophyllene directly activates CB2 receptors in the skin, inhibiting the opening of TRPV1 channels in peripheral sensory nerve endings, reducing the release of substance P and calcitonin gene-related peptide (CGRP), thereby reducing burning sensation and neurogenic inflammation. 4-tert-Butylcyclohexanol (4-t-Butylcyclohexanol) is a synthetic alicyclic alcohol that inhibits the transmission of stinging signals triggered by capsaicin, heat, or pH changes by blocking the calcium ion channel of the TRPV1 receptor. Its molecular structure binds to the extracellular domain of TRPV1, reducing the probability of channel opening, with the effect lasting 8-12 hours. It can effectively relieve skin discomfort such as redness, itching, and stinging. Flaxseed (Linum usitatissimum) contains lignans (such as secoisolariciresinol diglucoside, SDG) and α-linolenic acid. SDG competitively binds to the NK-1 receptor, blocking the interaction between substance P and the receptor, inhibiting the release of histamine and TNF-α from mast cells, reducing erythema and itching. α-linolenic acid regulates epidermal lipid metabolism, promotes ceramide synthesis, and repairs the integrity of the water-oil film. The present invention speculates that, based on the aforementioned effects of the above components and other effects not mentioned in the present invention, there is an unpredictable synergistic effect between the components, so that when compounded within the mass parts range given in the present invention, the comprehensive effect is excellent and a significant effect is achieved.

[0010] As a preferred embodiment of the mood beauty composition of the present invention, the mood beauty composition comprises the following components in parts by mass: 0.5-2 parts of red clover extract, 0.2-0.5 parts of copaiba resin, 0.4-2 parts of 4-tert-butyl cyclohexanol, and 0.3-0.8 parts of flaxseed extract.

[0011] The present invention has found that when the mass fractions of the components in the mood beauty composition are further selected to be within the above range, the comprehensive performance of the obtained product is better.

[0012] As a preferred embodiment of the mood beauty composition of the present invention, the mass ratio of the red clover extract to 4-tert-butyl cyclohexanol is 1:(0.8-1).

[0013] Illustratively, the mass ratio of the red clover extract to 4-tert-butyl cyclohexanol can be any point value or any two point range values ​​between 1:(0.8-1), such as 1:0.8, 1:0.9, 1:1, etc.

[0014] As a preferred embodiment of the mood beauty composition of the present invention, the mass ratio of the copaiba resin to the linseed extract is 1:(0.6-4).

[0015] Illustratively, the mass ratio of the copaiba resin to the flaxseed extract can be any point value or any two point range values ​​between 1:(0.6-4), such as 1:0.6, 1:0.7, 1:0.8, 1:0.9, 1:1, 1:2, 1:3, 1:4, etc.

[0016] As a preferred embodiment of the mood beauty composition of the present invention, the mass percentage of the sum of the masses of the copaiba resin and the linseed extract is 20-48% based on the total mass of the mood beauty composition.

[0017] Illustratively, based on the total mass of the mood beauty composition, the mass percentage of the sum of the masses of the copaiba resin and the flaxseed extract can be any point value or any two-point range value between 20-48%, such as 20%, 25%, 30%, 35%, 40%, 45%, 48%, etc.

[0018] Preferably, based on the total mass of the mood beauty composition, the mass percentage of the sum of the masses of copaiba resin and linseed extract is 25-30%.

[0019] The present invention has found that further limiting the mass percentage range of the sum of the masses of copaiba resin and linseed extract in the total mass of the mood beauty composition can achieve better results, and the resulting product has better ability to reduce skin sensitivity, enhance the skin barrier, and have anti-aging capabilities.

[0020] As a preferred embodiment of the mood beauty composition of the present invention, the preparation method of the red clover extract comprises the following steps: taking the dried, crushed and sieved red clover and performing supercritical carbon dioxide extraction to obtain the red clover extract;

[0021] In the supercritical carbon dioxide extraction, the co-solvent is 90-95 wt% ethanol aqueous solution, the extraction temperature is 40-60° C., and the extraction pressure is 25-35 MPa.

[0022] The present invention has found that by using supercritical carbon dioxide for extraction, introducing ethanol aqueous solution as a co-solvent during the extraction process, and limiting the extraction temperature and extraction pressure within the above-mentioned range, the solubility of the system for the target compound in red clover can be effectively enhanced, so that when it is compounded with other components, the overall effect of the obtained product is better.

[0023] For example, the extraction temperature may be any value between 40-60°C or any two values ​​within a range, such as 40°C, 45°C, 50°C, 55°C, 60°C, etc. The extraction pressure may be any value between 25-35 MPa or any two values ​​within a range, such as 25 MPa, 28 MPa, 30 MPa, 32 MPa, 35 MPa, etc.

[0024] As a preferred embodiment of the mood beauty composition of the present invention, in the supercritical carbon dioxide extraction, the mass percentage of the co-solvent is 5-10% based on the total mass of the supercritical carbon dioxide and the co-solvent.

[0025] For example, the mass percentage of the co-solvent can be any point value between 5-10% or any two points in the range, such as 5%, 6%, 7%, 8%, 9%, 10%, etc.

[0026] As a preferred embodiment of the mood beauty composition of the present invention, in the supercritical carbon dioxide extraction, the extraction flow rate of the supercritical carbon dioxide is 1-2 kg / h, and the extraction flow rate of the co-solvent is 0.053-0.222 kg / h.

[0027] As a preferred embodiment of the mood beauty composition of the present invention, the supercritical carbon dioxide extraction includes static extraction and dynamic extraction. The static extraction time is 20-30 minutes, and the dynamic extraction time is 1-2 hours.

[0028] It should be noted that the static extraction refers to the process in which the supercritical carbon dioxide and co-solvent remain stationary in a closed extraction kettle without solvent flow. Only high pressure and temperature are used to fully contact and infiltrate all solvents with the raw materials to be extracted. Under the pressure and temperature of the extraction, the supercritical carbon dioxide and co-solvent penetrate into the cells of the raw materials to be extracted, destroying the cell wall structure and releasing the active ingredients.

[0029] It should be noted that the dynamic extraction means that after the static extraction is completed, the solvent pump is started to continuously pump fresh supercritical carbon dioxide at a flow rate of 1-2 kg / h and the co-solvent at a flow rate of 0.053-0.222 kg / h into the extraction kettle, carrying the dissolved active ingredients of the raw material to be extracted out to the separator.

[0030] It should be noted that after the active ingredient to be extracted flows into the separator during dynamic extraction, the pressure in the separator is reduced from the extraction pressure of 25-35 MPa to atmospheric pressure (0.1 MPa), causing the supercritical carbon dioxide to rapidly vaporize, resulting in a sudden drop in density and a loss of solubility. The extracted active ingredient and the co-solvent are then precipitated and deposited at the bottom of the separator. A valve is then opened to collect the mixed liquid of the active ingredient and co-solvent. The mixed liquid is then spun off the co-solvent in a rotary evaporator at a temperature of 45-55°C, a vacuum of 30 mbar, and a rotation speed of 100-200 rpm to obtain the red clover extract.

[0031] As a preferred embodiment of the mood beauty composition of the present invention, the preparation method of the flaxseed extract includes the following steps: taking dried, crushed and sieved flaxseeds and adding them to deionized water, then adding cellulase and pectinase for enzymatic hydrolysis, then inactivating the enzymes and collecting the enzymatic hydrolysis products and homogenizing them under high pressure, and finally cooling and reducing the pressure, filtering, and collecting the filtrate to obtain the flaxseed extract.

[0032] The present invention has found that when the flaxseed extract is prepared by the above method and then used in a mood beauty composition, the obtained product has better comprehensive effects.

[0033] As a preferred embodiment of the mood beauty composition of the present invention, during the enzymatic hydrolysis process, the enzymatic hydrolysis temperature is 40-60° C., the enzymatic hydrolysis time is 1-2 h, and the enzymatic hydrolysis pH value is 5.0-7.0.

[0034] For example, the enzymatic hydrolysis temperature can be any value between 40-60°C or any two values ​​within a range, such as 40°C, 45°C, 50°C, 55°C, 60°C, etc. The enzymatic hydrolysis time can be any value between 1-2 hours or any two values ​​within a range, such as 1 hour, 1.5 hours, 2 hours, etc. The enzymatic hydrolysis pH can be any value between 5.0-7.0 or any two values ​​within a range, such as 5.0, 6.0, 7.0, etc.

[0035] Preferably, during the enzymatic hydrolysis process, the enzymatic hydrolysis temperature is 45-50° C., the enzymatic hydrolysis time is 2 h, and the enzymatic hydrolysis pH value is 6.0.

[0036] It should be noted that the pH value of the enzymatic hydrolysis can be adjusted by using citric acid and phosphate buffer solution.

[0037] As a preferred embodiment of the mood beauty composition of the present invention, during the enzymatic hydrolysis process, the mass ratio of flaxseed to deionized water is 1:(10-20).

[0038] Illustratively, during the enzymatic hydrolysis process, the mass ratio of flaxseed to deionized water may be any point value or any range of two points between 1:(10-20), such as 1:10, 1:15, 1:20, etc.

[0039] As a preferred embodiment of the mood beauty composition of the present invention, during the enzymatic hydrolysis process, the enzymatic activity of cellulase is 30-50 U, and the enzymatic activity of pectinase is 20-30 U per gram of flaxseed.

[0040] For example, the cellulase activity per gram of flaxseed can be any value or any range of values ​​between 30-50 U, such as 30 U, 35 U, 40 U, ​​45 U, 50 U, etc. The pectinase activity per gram of flaxseed can be any value or any range of values ​​between 20-30 U, such as 20 U, 25 U, 30 U, etc.

[0041] Preferably, during the enzymatic hydrolysis process, the enzymatic activity of cellulase is 30-40 U, ​​and the enzymatic activity of pectinase is 20-25 U per gram of flaxseed.

[0042] As a preferred embodiment of the mood beauty composition of the present invention, the enzyme inactivation is carried out by heat preservation at 85-95° C. for 8-12 minutes.

[0043] As a preferred embodiment of the mood beauty composition of the present invention, during the high-pressure homogenization process, the pressure is 100-200 MPa, the temperature is 85-95° C., the time is 2-5 minutes, and the number of times is 2-3 times.

[0044] For example, the pressure can be any value or any range of values ​​between 100-200 MPa, such as 100 MPa, 120 MPa, 140 MPa, 160 MPa, 180 MPa, 200 MPa, etc. The temperature can be any value or any range of values ​​between 85-95° C., such as 85° C., 90° C., 95° C., etc. The time can be any value or any range of values ​​between 2-5 minutes, such as 2 minutes, 3 minutes, 4 minutes, 5 minutes, etc.

[0045] The present invention has found that when the above-mentioned high-pressure homogenization parameters are used, the mechanical shear force in the high-pressure homogenization can be used to emulsify the oil-soluble components and the water-soluble components with each other, thereby obtaining emulsified nanoparticles with an average particle size of 20-100 nm, effectively enhancing the interaction between the oil-soluble components and the water-soluble components, improving the product stability, and promoting the transdermal absorption of the product, thereby obtaining a product with better overall performance.

[0046] Preferably, during the high-pressure homogenization process, the pressure is 100-150 MPa, the time is 5 minutes, and the number of times is 3 times.

[0047] It is further preferred that the parameters are within the above ranges, which can obtain emulsified nanoparticles with an average particle size of 20-50 nm, thereby obtaining a product with better overall effect.

[0048] It should be noted that the average particle size of the emulsified nanoparticles is obtained through scanning electron microscopy (SEM) testing.

[0049] In a second aspect of the present invention, the present invention provides use of the mood beauty composition in preparing skin care products.

[0050] The emotional beauty composition provided by the present invention can act from both physiological and emotional aspects, effectively relieve stress, and enhance emotional pleasure, thereby effectively reducing skin sensitivity, improving skin redness, and enhancing skin barrier capacity and achieving good anti-aging effects, and can therefore be widely used in the preparation of skin care products.

[0051] As a preferred embodiment of the application of the present invention, the skin care product includes any one of lotion, emulsion, cream, mask, essence, and spray.

[0052] In the third aspect of the present invention, the present invention provides an emulsion comprising the following components in mass percentage: 0.3-5% of the emotional beauty composition of the present invention, 0.1-0.5% of a thickener, 3-10% of a moisturizer, 0.05-0.5% of a pH regulator, 0.1-10% of an emulsifier, 0.5-5% of a preservative, and the balance deionized water.

[0053] As a preferred embodiment of the emulsion of the present invention, the emulsion comprises the following components in mass percentage: 2-3% of the emotional beauty composition of the present invention, 0.1-0.3% of a thickener, 4-6% of a moisturizer, 0.1-0.2% of a pH regulator, 6-8% of an emulsifier, 4-4.5% of a preservative, and the balance of deionized water.

[0054] As a preferred embodiment of the emulsion of the present invention, the thickener includes at least one of polyacrylate crosspolymer-6, carbomer, carrageenan, gellan gum, xanthan gum, microcrystalline cellulose, cellulose gum, ethyl cellulose, tara gum, guar gum, and ammonium acryloyldimethyltaurate / VP copolymer.

[0055] As a preferred embodiment of the emulsion of the present invention, the moisturizing agent includes at least one of glycerin, D-panthenol, vitamin B5, 1,3-butylene glycol, 1,2-hexanediol, 1,3-propylene glycol, sodium hyaluronate, tremella polysaccharide, trehalose, betaine, allantoin, and low-molecular sodium hyaluronate.

[0056] As a preferred embodiment of the emulsion of the present invention, the pH regulator includes at least one of disodium edetate and arginine.

[0057] As a preferred embodiment of the emulsion of the present invention, the emulsifier includes at least one of caprylic / capric triglyceride, C14-22 alcohol, C12-20 alkyl glucoside, cetearyl glucoside, isononyl isononanoate, pentaerythritol tetra(ethylhexanoate), polydimethylsiloxane, PEG-40 hydrogenated castor oil, stearyl alcohol, sucrose stearate, hydroxystearic acid, polymethylsilsesquioxane, pentaerythritol distearate, sucrose stearate, and polydimethylsiloxane.

[0058] As a preferred embodiment of the emulsion of the present invention, the preservative includes at least one of p-hydroxyacetophenone and polyol.

[0059] Compared with the prior art, the present invention has the following beneficial effects:

[0060] By selecting components in appropriate mass proportions, the present invention has a good synergistic effect between the components, can act from both physiological and emotional aspects, effectively reduce skin sensitivity, improve sensitive symptoms such as skin redness, and enhance the skin's barrier capacity. At the same time, the emotional beauty composition also has excellent anti-aging ability. BRIEF DESCRIPTION OF THE DRAWINGS

[0061] Figure 1 This is the erythema condition on the face of volunteers in Application Example 1 and Comparative Application Example 1 on day 0 and day 28. DETAILED DESCRIPTION

[0062] In order to better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0063] Unless otherwise specified, the reagents, methods, and equipment used in the present invention are all conventional reagents, methods, and equipment in the art; and unless otherwise specified, the raw materials used in parallel experiments are from the same batch of raw materials.

[0064] Copaiba resin: Copaiba essential oil was purchased from Guangzhou Fuyicheng Biotechnology Co., Ltd.

[0065] 4-tert-Butylcyclohexanol: SymSitive 1609, purchased from Symrise.

[0066] Red Clover Extract 1: Homemade, the preparation method includes the following steps:

[0067] 20 kg of red clover was dried and crushed, and the mixture was passed through a 60-mesh sieve. The sieve residue was collected and placed in an extraction kettle for supercritical carbon dioxide extraction. The parameters of the extraction process were as follows: extraction pressure of 35 MPa, temperature of 45° C., co-solvent of 95 wt% ethanol aqueous solution, the addition amount of which was 5% of the total solvent (mixed system of supercritical carbon dioxide and co-solvent), supercritical carbon dioxide extraction flow rate of 1.25 kg / h, co-solvent extraction flow rate of 0.066 kg / h, static extraction time of 30 min, and dynamic extraction time of 2 h. After the extraction, the temperature and pressure were lowered, the bottom liquid was collected, and the co-solvent was removed by rotary evaporation at a temperature of 50° C., a vacuum of 30 mbar, and a rotation speed of 150 rpm to obtain red clover extract 1.

[0068] Red clover extract 2: homemade. The difference between the preparation method and Red clover extract 1 lies in the different parameters during the extraction process, specifically: extraction pressure of 30 MPa, temperature of 50°C, co-solvent of 95 wt% ethanol aqueous solution, the addition amount is 8% of the total solvent mass, supercritical carbon dioxide extraction flow rate is 1.25 kg / h, co-solvent extraction flow rate is 0.109 kg / h, static extraction time is 30 min, and dynamic extraction time is 2 h;

[0069] Red clover extract 3: homemade. The difference between the preparation method and Red clover extract 1 is that the extraction pressure is 40 MPa and the temperature is 65°C.

[0070] Red clover extract 4: homemade. The difference between the preparation method and Red clover extract 1 is that the extraction pressure is 20 MPa and the temperature is 35°C.

[0071] Red Clover Extract 5: Homemade, the preparation method includes the following steps:

[0072] (1) 20 kg of red clover was dried and crushed, passed through a 60-mesh sieve, and the sieve residue was collected and added to a natural deep eutectic solvent aqueous solution (the mass percentage of the natural deep eutectic solvent was 75% based on the total mass of the natural deep eutectic solvent aqueous solution; the natural deep eutectic solvent was composed of β-glucan and betaine in a mass ratio of 1:1; the mass volume ratio of the sieve residue to the natural deep eutectic solvent aqueous solution was 1 g:20 mL) and ultrasonically extracted at a power of 200 W and 45 ° C for 35 min.

[0073] (2) After the ultrasonic extraction, the mixture was centrifuged, the supernatant was collected and concentrated under reduced pressure to obtain the red clover extract 5.

[0074] Flaxseed extract 1: Homemade, the preparation method includes the following steps:

[0075] (1) Flax seeds were dried at 45°C to constant weight and then crushed through an 80-mesh sieve. 50 g of the sieve residue was collected and added to 20 times the mass of deionized water and mixed evenly. Cellulase (the activity of cellulase was 30 U per gram of sieve residue) and pectinase (the activity of cellulase was 20 U per gram of sieve residue) were then added. The pH value of the system was then adjusted to 5.0 with citric acid and phosphate buffer solution. The system was then heated to 55°C for enzymatic hydrolysis for 2 h. After the enzymatic hydrolysis was completed, the system was heated to 85°C for 10 min to inactivate the enzyme. The system was then filtered and the filtrate was collected to obtain the enzymatic hydrolysis product.

[0076] (2) adding the enzymatic hydrolyzate to a high-pressure homogenizer and homogenizing at 100 MPa and 85°C for 5 min, repeating this process three times to obtain emulsified nanoparticles with an average particle size of 100 nm. Subsequently, the pressure was reduced, vacuum filtered, and the filtrate was collected to obtain flaxseed extract 1;

[0077] Flaxseed extract 2: Homemade, the preparation method includes the following steps:

[0078] (1) Flax seeds were dried at 45°C to constant weight and then crushed through an 80-mesh sieve. 50 g of the sieve residue was collected and added to 20 times the mass of deionized water and mixed evenly. Cellulase (the activity of cellulase was 40 U per gram of sieve residue) and pectinase (the activity of cellulase was 25 U per gram of sieve residue) were then added. The pH value of the system was then adjusted to 7.0 with citric acid and phosphate buffer solution. The system was then heated to 45°C for enzymolysis for 2 h. After the enzymolysis was completed, the system was heated to 85°C for 10 min to inactivate the enzyme. The system was then filtered and the filtrate was collected to obtain the enzymolysis product.

[0079] (2) adding the enzymatic hydrolyzate to a high-pressure homogenizer and homogenizing at 150 MPa and 85°C for 5 min, repeating three times to obtain emulsified nanoparticles with an average particle size of 50 nm, then reducing the pressure, vacuum filtering, and collecting the filtrate to obtain flaxseed extract 2;

[0080] Flaxseed extract 3: homemade. The only difference in preparation method from flaxseed extract 1 is that no pectinase is added, and the cellulase activity is 50 U per gram of sieve residue.

[0081] Flaxseed extract 4: homemade. The only difference in preparation method from flaxseed extract 1 is that no cellulase is added, and the pectinase activity is 50 U per gram of sieve residue.

[0082] Flaxseed Extract 5: Homemade. The only difference in the preparation method from Flaxseed Extract 1 was homogenization at 200 MPa. The average particle size of the resulting emulsified nanoparticles was 20 nm.

[0083] Flaxseed Extract 6: Homemade. The only difference in the preparation method from Flaxseed Extract 1 is that high-pressure homogenization is not performed. The resulting enzymatic hydrolyzate is concentrated to 5% of the initial volume of the enzymatic hydrolyzate to obtain Flaxseed Extract 6.

[0084] Flaxseed extract 7: Homemade, the preparation method includes the following steps:

[0085] (1) Flax seeds were dried at 45°C to constant weight and then pulverized through an 80-mesh sieve. The sieve residue was collected and placed in an extraction kettle for supercritical carbon dioxide extraction. The extraction parameters were as follows: extraction pressure of 35 MPa, temperature of 45°C, extraction flow rate of 1.25 kg / h, and dynamic extraction for 2 h.

[0086] (2) After the supercritical carbon dioxide extraction is completed, the temperature and pressure are lowered, the extract is collected and concentrated and dried to obtain flaxseed extract 7.

[0087] Examples 1-8 and Comparative Examples 1-7

[0088] The embodiments and comparative examples of the present invention provide a mood beauty composition, the components (parts by weight) of the composition are shown in Table 1;

[0089] Wherein, W1 represents the mass ratio of red clover extract to 4-tert-butyl cyclohexanol, W2 represents the mass ratio of copaiba resin and linseed extract, and W3 represents the mass percentage of the sum of the masses of copaiba resin and linseed extract based on the total mass of the mood beauty composition;

[0090] Table 1

[0091]

[0092]

[0093] The preparation method of the mood beauty composition provided in Example 1 is: mixing the components to obtain the mood beauty composition.

[0094] The preparation methods of the mood beauty compositions provided in Examples 2-8 and Comparative Examples 1-7 are consistent with that in Example 1; if relevant components are not present, they may be omitted.

[0095] Example 9

[0096] The embodiment of the present invention provides a mood beauty composition. The only difference between the mood beauty composition and the embodiment 1 is that the red clover extract 2 is used instead of the red clover extract 1.

[0097] Example 10

[0098] The embodiment of the present invention provides a mood beauty composition. The only difference between the mood beauty composition and embodiment 1 is that the red clover extract 3 is used instead of the red clover extract 1.

[0099] Example 11

[0100] The embodiment of the present invention provides a mood beauty composition. The only difference between the mood beauty composition and the embodiment 1 is that the red clover extract 4 is used instead of the red clover extract 1.

[0101] Example 12

[0102] The embodiment of the present invention provides a mood beauty composition. The only difference between the mood beauty composition and the embodiment 1 is that the red clover extract 5 is used instead of the red clover extract 1.

[0103] Example 13

[0104] This embodiment of the present invention provides a mood beauty composition. The only difference between the mood beauty composition and Example 1 is that the flaxseed extract 2 is used instead of the flaxseed extract 1.

[0105] Example 14

[0106] The embodiment of the present invention provides a mood beauty composition. The only difference between the mood beauty composition and the embodiment 1 is that the flaxseed extract 3 is used instead of the flaxseed extract 1.

[0107] Example 15

[0108] The embodiment of the present invention provides a mood beauty composition. The only difference between the mood beauty composition and the embodiment 1 is that the flaxseed extract 1 is replaced by the flaxseed extract 4.

[0109] Example 16

[0110] This embodiment of the present invention provides a mood beauty composition. The only difference between the mood beauty composition and Example 1 is that flaxseed extract 5 is used instead of flaxseed extract 1.

[0111] Example 17

[0112] The embodiment of the present invention provides a mood beauty composition. The only difference between the mood beauty composition and the embodiment 1 is that the flaxseed extract 1 is replaced by the flaxseed extract 6.

[0113] Example 18

[0114] This embodiment of the present invention provides a mood beauty composition. The only difference between the mood beauty composition and Example 1 is that flaxseed extract 7 is used instead of flaxseed extract 1.

[0115] Comparative Example 8

[0116] The comparative example of the present invention provides a mood beauty composition, the only difference between the mood beauty composition and Example 1 is that alfalfa extract (purchased from Shanghai Jiakai Biotechnology Co., Ltd., trade name Clover) is used instead of red clover extract 1.

[0117] Comparative Example 9

[0118] The comparative example of the present invention provides a mood beauty composition, the only difference between the mood beauty composition and Example 1 is that myrrh resin (purchased from Shaanxi Sinuo Te Biotechnology Co., Ltd., trade name Myrrh Extract) is used instead of Cuban rubber tree resin.

[0119] Comparative Example 10

[0120] The comparative example of the present invention provides a mood beauty composition, the only difference between the mood beauty composition and Example 1 is that citrus fruit extract (purchased from Suzhou Nakang Biotechnology Co., Ltd., with the trade name of Citrus Aurantium) is used instead of 4-tert-butyl cyclohexanol.

[0121] Comparative Example 11

[0122] The comparative example of the present invention provides a mood beauty composition, the only difference between the mood beauty composition and Example 1 is that sesame seed extract (purchased from Guangzhou Dizhou Biotechnology Co., Ltd.) is used instead of flaxseed extract 1.

[0123] Application Examples 1-20, Comparative Application Examples 1-11, and Blank Application Examples

[0124] The application examples, comparative application examples and blank application examples of the present invention provide an emulsion, the components (mass percentage) of the emulsion are shown in Table 2; wherein, the emotional beauty compositions used in Application Examples 1-18 are respectively the emotional beauty compositions prepared in Examples 1-18, for example, the emotional beauty composition used in Application Example 1 is the emotional beauty composition in Example 1, the emotional beauty composition used in Application Example 2 is the emotional beauty composition in Example 2, and so on; the emotional beauty compositions used in Comparative Application Examples 1-11 are respectively the emotional beauty compositions prepared in Comparative Examples 1-11; the emotional beauty compositions used in Application Examples 19-20 are the emotional beauty compositions prepared in Example 1;

[0125] Table 2

[0126]

[0127]

[0128] The preparation method of the emulsion provided in Application Example 1 comprises the following steps:

[0129] Put each component in phase A into water in turn, mix homogeneously after xanthan gum is fully swelled, heat it separately with phase B until the solid matter melts evenly, mix homogeneously and emulsify at 80°C, heat phase C until p-hydroxyacetophenone melts, add it to the emulsion and stir evenly, after the temperature drops to 40°C, add phase D and stir to form a uniform emulsion, finally add arginine to adjust the pH to obtain the emulsion.

[0130] The preparation methods of the emulsions provided in Application Examples 2-20, Comparative Application Examples 1-11 and Blank Application Examples are consistent with that in Application Example 1, and no relevant components are added.

[0131] Effect Example 1

[0132] Effect Examples of the Present Invention The effects of the mood beauty compositions prepared in Examples 1-18 and Comparative Examples 1-11 were investigated, including the following aspects:

[0133] 1. In vitro stress relief ability: The ability of the emotional beauty composition to relieve stress and reduce sensitivity in vitro was evaluated by testing its inhibitory effect on the stress hormone cortisol;

[0134] The cell line used was human keratinocyte HaCaT (Shanghai Yaji Biotechnology Co., Ltd.). The test conditions were: incubator temperature 37±1°C, humidity 90±5%, and carbon dioxide 5±1%. Cells were cultured and treated according to grouping, and then tested. The test method is as follows:

[0135] (1) Sample preparation: Each sample (emotional beauty composition prepared in Examples 1-18 and Comparative Examples 1-11) was dissolved in DMSO to form a sample solution, wherein the mass percentage of the sample in the sample solution was 10%;

[0136] (2) The cell suspension was inoculated into a 96-well cell culture plate at a density of 2000 cells per well, 100 μL of culture medium was added to each well, and cultured for 24 h;

[0137] (3) Induction and sampling: The supernatant was discarded, and 100 μL of DMEM medium (2105341, Gibco) containing 100 nM adrenocorticotropic hormone (ACTH) (MedChemExpress, HY-106373) was added to the control group, and 100 μL of medium containing 100 nM ACTH and 0.5 wt% sample solution was added to the sample group, and cultured for 24 h;

[0138] (4) Determination of cortisol content: The cell supernatant was extracted and the cortisol content of the cell supernatant of each group was detected using a human cortisol ELISA kit (Cat. No. BLL107923E, Baililai Biotechnology), and the cortisol reduction rate was calculated;

[0139] Cortisol reduction rate = (Cortisol对照组 -Cortisol 样品组 ) / Cortisol 对照组 ×100%; wherein, the lower the cortisol reduction rate, the stronger the ability of the combination to relieve stress; the results are shown in Table 3.

[0140] 2. Ability to reduce skin sensitivity in vitro: Detect the inhibitory effect of the emotional beauty composition on the skin pain receptor TRPV1 pathway and the inflammation-related receptor NK-1, and evaluate the efficacy of the emotional beauty composition in reducing sensitivity in vitro;

[0141] The cell line used was human keratinocyte HaCaT (Shanghai Yaji Biotechnology Co., Ltd.). The test conditions were: incubator temperature 37±1°C, humidity 90±5%, and carbon dioxide 5±1%. Cells were cultured and treated according to grouping, and then tested. The test method is as follows:

[0142] (1) Sample preparation: The sample (emotional beauty composition prepared in Examples 1-18 and Comparative Examples 1-11) was dissolved in DMSO to form a sample solution, wherein the mass percentage of the sample in the sample solution was 10%;

[0143] (2) The cell suspension was inoculated into a 96-well cell culture plate at a density of 2000 cells per well, 100 μL of culture medium was added to each well, and cultured for 24 h;

[0144] (3) Induction and sampling: The supernatant was discarded, and 100 μL of DMEM medium (2105341, Gibco) containing 4.6 μg / mL capsaicin solution as the stimulant was added to the control group, and 100 μL of medium containing 4.6 μg / mL capsaicin solution as the stimulant and 0.5 wt% sample solution was added to the sample group, and cultured for 24 h;

[0145] (4) Real-time fluorescence quantitative PCR was used to detect the expression levels of TRPV1 and NK-1R genes in HaCaT cells of each group, using GAPDH as the internal reference gene and the TRPV1 primer sequence used:

[0146] 5'-TGCAACCGGCTACTACAAGAAC-3',

[0147] 5'-AGGTTCCTGGAGCACCCATTCT-3',

[0148] The primer sequences of NK-1R used are:

[0149] 5'-TGACCACCTACCTACAGCAACT-3'

[0150] 5'-CAGCGTGGTCTGGATATGTATC-3',

[0151] The GAPDH primer sequences used are:

[0152] 5'-ACTTTGTCAAGCTCATTTCC-3'

[0153] 5′-TGCAGCGAACTTTATTGATG-3′;

[0154] The gene inhibition rate was then calculated, gene inhibition rate = |(target gene expression 对照组 - Target gene expression 样品组 )| / Target gene expression 对照组 *100%; wherein, the higher the inhibition rate of TRPV1 and NK-1R, the stronger the ability of the composition to reduce sensitivity; the obtained results are shown in Table 3.

[0155] Table 3

[0156]

[0157] As can be seen from Table 3, when the technical solution provided by the present invention is adopted, the obtained mood beauty composition has a good ability to reduce cortisol secretion and reduce skin sensitivity; specifically, the obtained product has a cortisol reduction rate of more than 43.02%, and the inhibition rates of TRPV1 and NK-1R are more than 63.42% and 37.83%, respectively;

[0158] As can be seen from Examples 1-8, the mass parts of the components and the mass ratio between the components, or the mass percentage of two components in the composition, will also affect the performance of the product. When the mass parts of the components are further selected within the preferred range given in the present invention, the overall performance of the obtained product is better. Specifically, the obtained product has a cortisol reduction rate of more than 60.92%, and an inhibition rate of more than 73.21% and more than 51.02% on TRPV1 and NK-1R, respectively. As can be seen from Examples 1 and Examples 9-12, and Examples 1 and Examples 13-18, the preparation methods of the red clover extract and the flaxseed extract will also affect the overall performance of the product to a certain extent.

[0159] It can be seen from Example 1 and Comparative Examples 1-7 that when one or two of the components are not added, or when they are not added and the other components are used to make up the difference, the effects of the present invention cannot be achieved; it can be seen from Example 1 and Comparative Examples 8-11 that when other similar ingredients are used instead, the corresponding effects of the present invention cannot be achieved.

[0160] Effect Example 2

[0161] The present invention explores the application effects of the emulsions prepared by application examples 1-20, comparative application examples 1-11 and blank application examples on the human body, comprising the following steps:

[0162] According to the "Technical Specifications for Safety of Cosmetics" (2015), 160 subjects aged 18-45 with sensitive skin were selected for the trial. Screening was based on a self-assessment questionnaire and a lactic acid sting score of 3 or higher. Subjects were randomly divided into 32 groups of 5. Volunteers applied the lotion (approximately 1-2g) to their entire face once daily, morning and evening. Data were collected on day 0 and day 28 of use. Afterward, the volunteers washed their faces with facial cleanser and sat quietly in an air-conditioned room at a temperature of 21±1°C and a humidity of 50±10% for 30 minutes. Before and after use, subjects photographed their under-eye wrinkles using VISIA-CR to analyze wrinkle area and calculate the improvement rate. Transepidermal water loss (TEWL) at the zygomatic bone was measured using a TewaMeter before and after use, and the improvement rate of facial TEWL was calculated. The subjects used the skin color test probe ColorimeterCL440 to measure their skin color before and after use, used a* value to represent the degree of facial redness, calculated the improvement rate of the facial redness a* value, and used the VISIA-CR instrument to photograph the facial redness.

[0163] The soothing ability is represented by the improvement rate of the facial redness a* value, the skin barrier repair ability is represented by the improvement of the TEWL value, and the anti-aging effect is represented by the improvement of the wrinkle area at the end of the eye. The improvement rate of each value is calculated as follows:

[0164] Improvement rate = |(T0 测量值 -T28 测量值 )| / T0 测量值 *100%; the results are shown in Table 4;

[0165] Table 4

[0166]

[0167] As can be seen from Table 4, when the technical solution provided by the present invention is adopted, the obtained mood beauty composition has good improvement of skin redness, enhanced skin barrier capacity and excellent anti-aging ability; specifically, the TEWL improvement rate, erythema improvement rate and wrinkle area improvement rate of the obtained emulsion are above 14.29%, above 19.38% and above 12.93%, respectively;

[0168] As can be seen from Application Examples 1-8, the mass fraction of the components of the mood beauty composition added to the emulsion, the mass ratio between the components, or the mass percentage of two components in the composition can also affect the performance of the emulsion. When the mass fractions of the components in the mood beauty composition are further selected to be within the preferred range given in the present invention, the overall performance of the resulting emulsion is better. Specifically, the TEWL improvement rate, erythema improvement rate, and wrinkle area improvement rate of the resulting emulsion are above 24.75%, above 30.64%, and above 21.04%, respectively. As can be seen from Application Examples 1 and Application Examples 9-12, and Application Examples 1 and Application Examples 13-18, the preparation methods of the red clover extract and the flaxseed extract can also affect the overall performance of the emulsion to a certain extent.

[0169] As can be seen from Application Example 1 and Comparative Application Examples 1-7, when one or two of the components are not added to the emotional beauty composition, or when other components are used to make up for the missing components, the effects of the present invention cannot be achieved; as can be seen from Application Example 1 and Comparative Application Examples 8-11, when other similar components are substituted, the corresponding effects of the present invention cannot be achieved. Furthermore, the facial redness of a random volunteer in Application Example 1 and Comparative Application Example 1 was photographed using the VISIA-CR instrument on days 0 and 28, as shown below. Figure 1 shown; from Figure 1 It can also be clearly seen that the emulsion prepared by the technical solution of the present invention can effectively improve facial redness and erythema.

[0170] Finally, it should be noted that the above embodiments are intended to illustrate the technical solutions of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A mood beauty composition for relieving stress and reducing skin sensitivity, characterized in that: The mood beauty composition comprises the following components in parts by weight: 0.1-5 parts of Trifolium repens extract, 0.01-1 parts of Copaiba resin, 0.1-5 parts of 4-tert-butyl cyclohexanol, and 0.01-2 parts of linseed extract; The preparation method of the red clover extract comprises the following steps: extracting the dried, crushed and sieved red clover with supercritical carbon dioxide to obtain the red clover extract; In the supercritical carbon dioxide extraction, the co-solvent is 90-95wt% ethanol aqueous solution, the extraction temperature is 40-60°C, and the extraction pressure is 25-35MPa; The preparation method of the flaxseed extract comprises the following steps: adding dried, crushed and sieved flaxseeds into deionized water, then adding cellulase and pectinase for enzymolysis, then inactivating the enzymes and collecting the enzymolysis products for high-pressure homogenization, finally cooling and reducing the pressure, filtering, and collecting the filtrate to obtain the flaxseed extract.

2. The mood beauty composition according to claim 1, characterized in that The mood beauty composition comprises the following components in parts by weight: 0.5-2 parts of Trifolium pratense extract, 0.2-0.5 parts of copaiba resin, 0.4-2 parts of 4-tert-butyl cyclohexanol, and 0.3-0.8 parts of linseed extract.

3. The mood beauty composition according to claim 1, characterized in that The mass ratio of the red clover extract to 4-tert-butyl cyclohexanol is 1:(0.8-1); And / or, the mass ratio of the copaiba resin to the flaxseed extract is 1:(0.6-4).

4. The mood beauty composition according to claim 1, characterized in that Based on the total mass of the mood beauty composition, the mass percentage of the sum of the masses of the copaiba resin and the flaxseed extract is 20-48%.

5. The mood beauty composition according to claim 1, characterized in that In the supercritical carbon dioxide extraction, the mass percentage of the co-solvent is 5-10% based on the total mass of the supercritical carbon dioxide and the co-solvent; and / or, in the supercritical carbon dioxide extraction, the extraction flow rate of the supercritical carbon dioxide is 1-2 kg / h, and the extraction flow rate of the co-solvent is 0.053–0.222 kg / h; And / or, the supercritical carbon dioxide extraction includes static extraction and dynamic extraction, the static extraction time is 20-30 minutes, and the dynamic extraction time is 1-2 hours.

6. The mood beauty composition according to claim 1, characterized in that During the enzymatic hydrolysis process, the enzymatic hydrolysis temperature is 40-60° C., the enzymatic hydrolysis time is 1-2 h, and the enzymatic hydrolysis pH value is 5.0-7.0; and / or, during the enzymatic hydrolysis process, the mass ratio of flaxseed to deionized water is 1:(10-20); And / or, during the enzymatic hydrolysis process, the enzymatic activity of cellulase is 30-50 U, and the enzymatic activity of pectinase is 20-30 U per gram of flaxseed; And / or, the enzyme inactivation is carried out by keeping the temperature at 85-95° C. for 8-12 minutes; And / or, during the high-pressure homogenization process, the pressure is 100-200 MPa, the temperature is 85-95° C., the time is 2-5 min, and the number of times is 2-3 times.

7. Use of the mood beauty composition according to any one of claims 1 to 6 in the preparation of skin care products.

8. An emulsion, characterized in that The emulsion comprises the following components in percentage by mass: 0.3-5% of the mood beauty composition according to any one of claims 1 to 6, 0.1-0.5% of a thickener, 3-10% of a moisturizer, 0.05-0.5% of a pH adjuster, 0.1-10% of an emulsifier, 0.5-5% of a preservative, and the balance of deionized water.

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