Processing method of rhodiola rosea decoction pieces

Abstract

The invention discloses a processing method of rhodiola rosea decoction pieces, and belongs to the technical field of traditional Chinese medicine processing. The processing method comprises the following steps: step 1, collecting fresh roots and rhizomes of rhodiola rosea, and washing with water to remove silt for later use; 2, the fresh roots and the rhizomes are placed in an aqueous solution containing 20-25 wt% of PEG-4000, 3-5 wt% of NaCl and 1-3 wt% of Na2SO4, the fresh roots and the rhizomes are soaked at the room temperature for 12-16 h, 300 nm ultraviolet light radiation is adopted for 10-20 s / h during room-temperature soaking, and the power density of ultraviolet light radiation is 0.1-0.2 mW / cm < 2 >; and then low-temperature soaking is conducted for 8-12 h at the temperature of 0-4 DEG C, in the low-temperature soaking process, 750nm red light radiation is adopted for 30-60 s / h, and the power density of red light radiation is 20-50 mW / cm < 2 >. The content of salidroside and salidrosin in the rhodiola rosea decoction pieces can be remarkably increased, and the medicinal value of rhodiola rosea can be further increased.

Description

Technical Field

[0001] The present invention relates to a processing method of Rhodiola root and rhizome slices, belonging to the technical field of traditional Chinese medicine processing. Background Art

[0002] Rhodiola is a traditional Chinese medicine, with rich sources and widely distributed in many regions, and is dug in a specific season. It is mainly distributed in Northeast, North, Southwest, Northwest of China, such as Jilin, Hebei, Gansu, Qingdao, Xinjiang, Sichuan, Yunnan, Guizhou, Tibet, etc. From July to September every year, when the stems and leaves gradually wither, it is the best time to dig Rhodiola. After drying or sun-drying, it becomes Rhodiola medicinal materials.

[0003] Rhodiola extract is the extract of Rhodiola medicinal materials. There are two main types of Rhodiola extracts in China: Rhodiola crenulata and Rhodiola rosea. Rhodiola crenulata is mainly produced in Tibet, and the main component extracted is salidroside; Rhodiola rosea is produced in Xinjiang, and the content of isomers such as rosavin is relatively high. Thus, it can be seen that the content of rhodiosin in the existing Rhodiola extracts is relatively low, which also indicates that the content of rhodiosin in Rhodiola medicinal materials and Rhodiola root and rhizome slices is even lower. How to obtain slices with high content of rhodiosin through processing is an urgent problem to be solved at present. Summary of the Invention

[0004] Aiming at the above problems, the purpose of the present invention is to provide a processing method of Rhodiola root and rhizome slices, which can significantly increase the content of salidroside and rhodiosin in the slices.

[0005] To solve the above technical problems, the technical solution adopted by the present invention is as follows:

[0006] A processing method of Rhodiola root and rhizome slices, comprising the following steps:

[0007] Step 1: From July to September, collect the fresh roots and rhizomes of Rhodiola crenulata, wash them with water to remove sediment, and set aside;

[0008] Step 2: Place the fresh roots and rhizomes in an aqueous solution containing 20-25wt% PEG-4000, 3-5wt% NaCl and 1-3wt% Na 2 SO 4 , first soak at room temperature for 12-16h, and during the room temperature soaking, irradiate with 300nm ultraviolet light for 10-20s / h; then soak at 0-4°C for 8-12h, and during the low-temperature soaking, irradiate with 750nm red light for 30-60s / h. The power density of ultraviolet light irradiation is 0.1-0.2mW / cm 2 ; the power density of red light irradiation is 20-50mW / cm 2 ; repeat 2-5 times like this;

[0009] Step 3: After soaking is completed, take out the product, soak it in pure water for at least 24 hours, then take it out, wash, dry, and slice to obtain the Rhodiola root and rhizome slices.

[0010] Preferably, the pure water is deionized water.

[0011] Preferably, drying includes air-drying or oven-drying.

[0012] For the Rhodiola root and rhizome slices obtained by the processing method of the present invention, the content of salidroside ≥ 4.85%, and the content of rhodiolin ≥ 1.01%.

[0013] Application of the processing method of the present invention in the preparation of Rhodiola root and rhizome slices with high content of salidroside and high content of rhodiolin.

[0014] Application of the Rhodiola root and rhizome slices of the present invention in the preparation of drugs for anti-altitude reaction, anti-fatigue, and treatment of Alzheimer's disease.

[0015] For the fresh roots and rhizomes of Rhodiola crenulata obtained in Step 1, wash them with water to remove sediment, air-dry, slice, take samples from the slices, crush them, accurately weigh an appropriate amount of the homogeneous sample, 0.5 g, accurate to 0.0001 g, place it in a 25 mL volumetric flask, add 20 mL of methanol, extract ultrasonically for 30 minutes, cool to room temperature, make up the volume to the scale with methanol, mix well, and filter through a 0.45 μm filter membrane to obtain Sample 1 for liquid chromatography analysis.

[0016] Similarly, take the Rhodiola root and rhizome slices obtained in Step 3 of the present invention, crush them, accurately weigh an appropriate amount of the homogeneous sample, 0.5 g, accurate to 0.0001 g, place it in a 25 mL volumetric flask, add 20 mL of methanol, extract ultrasonically for 30 minutes, cool to room temperature, make up the volume to the scale with methanol, mix well, and filter through a 0.45 μm filter membrane to obtain Sample 2 for liquid chromatography analysis.

[0017] It can be known from HPLC detection and analysis that the contents of salidroside and rhodiolin in the processed slices of the present invention are greatly increased.

[0018] Compared with the prior art, the present invention has the following advantages:

[0019] Most plants growing in the north and west will inevitably be affected by permanent or irregular drought climate. Through long-term natural selection and survival competition, they have self-formed a set of adaptation systems to the drought stress environment in terms of their external morphology, anatomical structure, and internal physiological and biochemical characteristics.

[0020] Rhodiola mainly grows in alpine regions at an altitude of 1,800 - 2,700 meters, commonly found in environments such as under forest slopes on mountains, grass slopes, and rock crevices. These areas usually have harsh conditions such as large temperature differences between day and night, strong ultraviolet rays, and low oxygen. Rhodiola has strong adaptability, can grow in barren soil, likes sunny environments, but is also drought-tolerant and cold-tolerant. The growth environment of Rhodiola has an important impact on its vitality and medicinal value. Its unique living environment endows Rhodiola with extremely strong vitality and special physiological activities, enabling it to thrive in extreme environments.

[0021] The flavonoid compound rhodiolin is an important secondary metabolite in plants of the genus Rhodiola.

[0022] Salidroside is a phenyl ethanol glycoside compound. Research has shown that salidroside has various pharmacological effects such as anti-fatigue, anti-aging, immune regulation, and free radical scavenging. Salidroside can inhibit the proliferation of tumor cells by interfering with cell metabolism and changing the properties of the cell coat. At the same time, it can also increase the conversion rate of T lymphocytes and the activity of phagocytes, enhance immunity, inhibit tumor growth, increase white blood cells, and resist microwave radiation.

[0023] Many chemical substances in Rhodiola, such as salidroside and rhodiolin, can have excellent neuroprotective effects on neurodegenerative diseases such as Alzheimer's disease (AD) through pharmacological means such as antioxidant, anti-inflammatory, and anti-apoptosis.

[0024] Thus, the present invention can significantly increase the contents of salidroside and rhodiolin in Rhodiola tablets, and can further improve the medicinal value of Rhodiola. Detailed implementation methods

[0025] The following further details the present invention in conjunction with specific embodiments. The following embodiments are only used to illustrate the present invention and are not used to limit the scope of the present invention.

[0026] The Rhodiola crenulata in the present invention was identified by Professor Huang Yun of Hebei Medical University as the fresh roots and rhizomes of Rhodiola crenulata (Hook.f.et Thoms.) H.Ohba, a plant of the Crassulaceae family.

[0027] Example 1

[0028] A method for processing Rhodiola tablets includes the following steps:

[0029] Step 1, from July to September, collect the fresh roots and rhizomes of Rhodiola crenulata (Hook.f.et Thoms.) H.Ohba, wash them with water to remove sediment, and set aside;

[0030] Step 2: Place the fresh roots and rhizomes in an aqueous solution containing 20 wt% PEG-4000, 4 wt% NaCl, and 2 wt% Na 2 SO 4 Soak at room temperature for 12 h first. During the room temperature soaking, irradiate with 300 nm ultraviolet light for 15 s / h, and the power density of the ultraviolet light irradiation is 0.1 mW / cm 2 ; Then soak at 0 °C for 10 h. During the low-temperature soaking, irradiate with 750 nm red light for 40 s / h, and the power density of the red light irradiation is 35 mW / cm 2 ; Repeat this process 3 times;

[0031] Step 3: After the soaking is completed, take out, soak in deionized water for 24 h, then take out, wash, dry, and slice to obtain the rhodiola officinalis slices.

[0032] For the rhodiola officinalis slices obtained by the processing method of this example, the content of salidroside is 4.85%, and the content of rhodiolin is 1.01%.

[0033] Application of the processing method of this example in the preparation of rhodiola officinalis slices with high content of salidroside and high content of rhodiolin.

[0034] Application of the rhodiola officinalis slices obtained in this example in the preparation of drugs for preventing altitude sickness, anti-fatigue, and treating Alzheimer's disease.

[0035] For the HPLC content determination steps, refer to the prior art, the method in the paper "Quality Evaluation of Rhodiola officinalis Based on Fingerprint Combined with Chemical Pattern Recognition and Identification of Its Adulterants" published in 2024, as follows:

[0036] 1. Standard stock solution of salidroside and rhodiolin reference substances (1 mg / mL): Weigh appropriate amounts of the two reference substances precisely, dissolve them with methanol and dilute to the mark to prepare reference substance stock solutions with a concentration of 1 mg / mL each. Store at below -18 °C, and the validity period is 6 months.

[0037] 2. Standard series working solutions: Pipette appropriate amounts of the reference substance standard stock solution and dilute with methanol to a series of concentrations of 0.01 mg / mL, 0.02 mg / mL, 0.05 mg / mL, 0.10 mg / mL, 0.20 mg / mL, and 0.50 mg / mL.

[0038] 3. High-performance liquid chromatograph: LC-20AT type high-performance liquid chromatograph (Shimadzu Corporation, Japan).

[0039] 4. Preparation of test solution: Weigh accurately an appropriate amount (0.5 g) of the homogeneous sample, accurate to 0.0001 g. Place it in a 25-mL volumetric flask, add 20 mL of methanol, extract ultrasonically for 30 min, cool to room temperature, make up the volume to the mark with methanol, mix well, and filter through a 0.45-μm filter membrane for high-performance liquid chromatography analysis.

[0040] The sample includes the following test articles:

[0041] Sample 1: 0.5 g of the dry sliced herb powder before processing of the present invention;

[0042] Sample 2: 0.5 g of the dry sliced herb powder after processing of the present invention.

[0043] 5. High-performance liquid chromatography conditions:

[0044] a) Chromatographic column: Waters Shield RP18 column (4.6×250 mm, 5 μm);

[0045] b) Column temperature: 35 °C;

[0046] c) Detector: Ultraviolet detector;

[0047] d) Detection wavelength: 275 nm;

[0048] e) Injection volume: 10 μL;

[0049] f) Flow rate: 1 mL / min;

[0050] g) Mobile phase: A is acetonitrile, B is 0.5% acetic acid aqueous solution. The gradient elution conditions are shown in Table 1.

[0051] Table 1 Gradient elution conditions of mobile phase

[0052]

[0053]

[0054] 6. Preparation of standard curve: Inject the standard series working solutions into the high-performance liquid chromatograph respectively, measure the peak areas of the corresponding substances. Take the concentration of the substance in the standard series working solutions as the abscissa and the response value of the peak area of the substance as the ordinate to plot the standard curve.

[0055] 7. Determination of sample solution: Inject the sample solution into the high-performance liquid chromatograph to obtain the peak area, and obtain the mass concentrations of salidroside and rhodioloside in the test solution according to the standard curve. The contents of salidroside and rhodioloside in the sample are calculated according to the standard curve or the following formula.

[0056]

[0057] In the formula:

[0058] X—the value of salidroside and rhodiolin in the test sample, unit: microgram per kilogram (μg / kg);

[0059] A 1 —the peak areas of salidroside and rhodiolin in the sample solution;

[0060] C—the concentration of salidroside and rhodiolin in the reference substance solution;

[0061] A 2 —the average peak area of the reference substance solution;

[0062] V—the value of the final volume of the test sample for constant volume, unit: milliliter (mL);

[0063] m—the value of the mass of the test sample, unit: gram (g).

[0064] The determination results of each test sample are shown in Table 2 below.

[0065] Table 2 Determination results of the test samples in Example 1

[0066]

[0067]

[0068] As can be seen from Table 2, for the processed cut pieces in this example, the content of salidroside increased by 2.4 times and the content of rhodiolin increased by 4.4 times. This significantly increased the contents of salidroside and rhodiolin in the Rhodiola cut pieces, so the medicinal value of Rhodiola can be further improved.

[0069] Example 2

[0070] A processing method for Rhodiola cut pieces, comprising the following steps:

[0071] Step 1: From July to September, collect the fresh roots and rhizomes of Rhodiola crenulata (Hook.f.et Thoms.) H.Ohba, wash with water to remove sediment, and set aside;

[0072] Step 2: Place the fresh roots and rhizomes in an aqueous solution containing 25 wt% PEG-4000, 3 wt% NaCl and 1 wt% Na 2 SO 4 soak at room temperature for 16 h first, and during the room temperature soaking, irradiate with 300 nm ultraviolet light for 10 s / h, and the power density of the ultraviolet light irradiation is 0.2 mW / cm 2 ; then soak at 4°C for 8 h, and during the low-temperature soaking, irradiate with 750 nm red light for 30 s / h, and the power density of the red light irradiation is 20 mW / cm2 ; Repeat this process 2 times;

[0073] Step 3: After the soaking is completed, take out the sample, soak it in deionized water for 48 h, take it out, wash it, dry it in an oven at 60 °C, and slice it to obtain the Rhodiola root and rhizome slices.

[0074] For the Rhodiola root and rhizome slices obtained by the processing method of this example, the content of salidroside is 4.93%, and the content of rhodiolin is 1.18%.

[0075] Application of the processing method of this example in the preparation of Rhodiola root and rhizome slices with high salidroside content and high rhodiolin content.

[0076] Application of the Rhodiola root and rhizome slices obtained in this example in the preparation of drugs for preventing altitude reaction, anti-fatigue, and treating Alzheimer's disease.

[0077] Example 3

[0078] A processing method for Rhodiola root and rhizome slices, comprising the following steps:

[0079] Step 1: From July to September, collect the fresh roots and rhizomes of Rhodiola crenulate (Hook.f.et Thoms.) H.Ohba, wash them with water to remove sediment, and set aside;

[0080] Step 2: Place the fresh roots and rhizomes in an aqueous solution containing 23 wt% PEG-4000, 5 wt% NaCl, and 3 wt% Na 2 SO 4 , soak at room temperature for 14 h first, and during the room temperature soaking, irradiate with 300 nm ultraviolet light for 20 s / h, and the power density of the ultraviolet light irradiation is 0.15 mW / cm 2 ; Then soak at 0 °C for 12 h, and during the low-temperature soaking, irradiate with 750 nm red light for 60 s / h, and the power density of the red light irradiation is 50 mW / cm 2 ; Repeat this process 5 times;

[0081] Step 3: After the soaking is completed, take out the sample, soak it in deionized water for 36 h, take it out, wash it, dry it in the air, and slice it to obtain the Rhodiola root and rhizome slices.

[0082] For the Rhodiola root and rhizome slices obtained by the processing method of this example, the content of salidroside is 4.89%, and the content of rhodiolin is 1.10%.

[0083] Application of the processing method of this example in the preparation of Rhodiola root and rhizome slices with high salidroside content and high rhodiolin content.

[0084] Application of the Rhodiola root and rhizome slices obtained in this example in the preparation of drugs for preventing altitude reaction, anti-fatigue, and treating Alzheimer's disease.

[0085] Comparative Example 1

[0086] The difference between this comparative example and Example 1 is only that:

[0087] This comparative example uses drought stress, that is, when soaking in the second step, an aqueous solution of 25 wt% PEG-4000 is used for soaking.

[0088] In the medicinal slices obtained in this comparative example, the content of salidroside is 3.03%, and the content of rhodioloside is 0.68%.

[0089] Comparative Example 2

[0090] The difference between this comparative example and Example 1 is only that:

[0091] This comparative example uses high-salt stress, that is, when soaking in the second step, an aqueous solution of 4 wt% NaCl and 2 wt% Na 2 SO 4 is used for soaking.

[0092] In the medicinal slices obtained in this comparative example, the content of salidroside is 2.94%, and the content of rhodioloside is 0.57%.

[0093] Comparative Example 3

[0094] The difference between this comparative example and Example 1 is only that:

[0095] When soaking in the second step of this comparative example, an aqueous solution of 25 wt% PEG-4000 and 6 wt% NaCl is used for soaking.

[0096] In the medicinal slices obtained in this comparative example, the content of salidroside is 4.08%, and the content of rhodioloside is 0.71%.

[0097] Comparative Example 4

[0098] The difference between this comparative example and Example 1 is only that:

[0099] When soaking in the second step of this comparative example, only room-temperature soaking is used. Soak at room temperature for 22 h, and irradiate with 300 nm ultraviolet light for 15 s / h during room-temperature soaking. The power density of ultraviolet light irradiation is 0.1 mW / cm 2 ; after soaking is completed, change the soaking solution and soak again at room temperature; repeat this 3 times.

[0100] In the medicinal slices obtained in this comparative example, the content of salidroside is 3.14%, and the content of rhodioloside is 0.54%.

[0101] Comparative Example 5

[0102] The difference between this comparative example and Example 1 is only that:

[0103] In the second step of soaking in this comparative example, only low-temperature soaking was adopted. Soaking was carried out at 0 °C for 22 h. During low-temperature soaking, 750 nm red light was irradiated for 40 s / h, and the power density of red light irradiation was 35 mW / cm 2 ; After soaking was completed, the soaking solution was changed and low-temperature soaking was carried out again; this was repeated 3 times.

[0104] In the rhodiola root and rhizome slices obtained in this comparative example, the content of salidroside was 3.22%, and the content of rhodiolin was 0.58%.

[0105] Comparative Example 6

[0106] The difference between this comparative example and Example 1 is only that:

[0107] In the second step of soaking in this comparative example, only soaking was carried out without irradiation. That is, the fresh roots and rhizomes were placed in an aqueous solution containing 20 wt% PEG-4000, 4 wt% NaCl and 2 wt% Na 2 SO 4 ; Soak at room temperature for 12 h first; then soak at 0 °C for 10 h; repeat this 3 times.

[0108] In the rhodiola root and rhizome slices obtained in this comparative example, the content of salidroside was 2.98%, and the content of rhodiolin was 0.45%.

[0109] Comparative Example 7

[0110] The difference between this comparative example and Example 1 is only that:

[0111] In the second step of soaking in this comparative example, only ultraviolet light irradiation was adopted. That is, the fresh roots and rhizomes were placed in an aqueous solution containing 20 wt% PEG-4000, 4 wt% NaCl and 2 wt% Na 2 SO 4 ; Soak at room temperature for 12 h first, and during room temperature soaking, 300 nm ultraviolet light was irradiated for 15 s / h, and the power density of ultraviolet light irradiation was 0.1 mW / cm 2 ; Then soak at 0 °C for 10 h, and during low-temperature soaking, 300 nm ultraviolet light was irradiated for 15 s / h, and the power density of ultraviolet light irradiation was 0.1 mW / cm 2 ; Repeat this 3 times.

[0112] In the rhodiola root and rhizome slices obtained in this comparative example, the content of salidroside was 4.21%, and the content of rhodiolin was 0.72%.

[0113] Comparative Example 8

[0114] The difference between this comparative example and Example 1 is only that:

[0115] In the second step of this comparative example during soaking, only red light irradiation was used. That is, the fresh roots and rhizomes were placed in an aqueous solution containing 20 wt% PEG-4000, 4 wt% NaCl, and 2 wt% Na 2 SO 4 . First, soak at room temperature for 12 h. During the room temperature soaking, irradiate with 750 nm red light for 40 s / h, and the power density of the red light irradiation is 35 mW / cm 2 ; then soak at 0 °C for 10 h. During the low-temperature soaking, irradiate with 750 nm red light for 40 s / h, and the power density of the red light irradiation is 35 mW / cm 2 ; repeat this 3 times.

[0116] In the prepared slices of this comparative example, the content of salidroside is 4.08%, and the content of rhodioloside is 0.75%.

[0117] Comparative Example 9

[0118] The difference between this comparative example and Example 1 is only that:

[0119] In the second step of this comparative example during soaking, red light irradiation was used during room temperature soaking, and ultraviolet light irradiation was used during low-temperature soaking. That is, the fresh roots and rhizomes were placed in an aqueous solution containing 20 wt% PEG-4000, 4 wt% NaCl, and 2 wt% Na 2 SO 4 . First, soak at room temperature for 12 h. During the room temperature soaking, irradiate with 750 nm red light for 40 s / h, and the power density of the red light irradiation is 35 mW / cm 2 ; then soak at 0 °C for 10 h. During the low-temperature soaking, irradiate with 300 nm ultraviolet light for 15 s / h, and the power density of the ultraviolet light irradiation is 0.1 mW / cm 2 ; repeat this 3 times.

[0120] In the prepared slices of this comparative example, the content of salidroside is 3.81%, and the content of rhodioloside is 0.66%.

[0121] It should be understood that, in order to streamline the present disclosure and assist in understanding one or more of the various inventive aspects, in the above description of the exemplary embodiments of the present invention, the various features of the present invention are sometimes grouped together into a single embodiment or the description thereof. However, the disclosed method should not be construed as reflecting the intention that the claimed invention requires more features than are expressly recited in each claim. Rather, as reflected by the claims, the inventive aspects lie in less than all the features of the foregoing disclosed embodiments. Thus, the claims following the detailed description are hereby expressly incorporated into the detailed description, where each claim stands on its own as a separate embodiment of the present invention.

[0122] Although the present invention has been described based on a limited number of embodiments, those skilled in the art in this technical field will understand, based on the above description, that other embodiments can be envisioned within the scope of the present invention thus described. In addition, it should be noted that the language used in this specification is mainly selected for the purpose of readability and teaching, rather than for the purpose of explaining or limiting the subject matter of the present invention. Therefore, many modifications and variations will be obvious to those of ordinary skill in the art without departing from the scope and spirit of the appended claims. For the scope of the present invention, the disclosure of the present invention is illustrative rather than restrictive, and the scope of the present invention is defined by the appended claims.

[0123] The above are only the preferred embodiments of the present invention. It should be pointed out that for those of ordinary skill in the art in this technical field, several improvements and refinements can be made without departing from the principle of the present invention, and these improvements and refinements should also be regarded as within the protection scope of the present invention.

Claims

1. A method for preparing Rhodiola rosea slices, characterized in that: The following steps are involved: Step 1, collect fresh roots and rhizomes of Rhodiola rosea, wash with water to remove mud and sand, and set aside; Step 2: Place the fresh roots and rhizomes in an aqueous solution containing 20-25wt% PEG-4000, 3-5wt% NaCl and 1-3wt% Na2SO4, soak them at room temperature for 12-16h, and irradiate them with 300nm ultraviolet light for 10-20s / h during soaking at room temperature. The power density of the ultraviolet light is 0.1-0.2mW / cm 2 ; Then soak at 0-4℃ for 8-12h. During low temperature soaking, use 750nm red light radiation for 30-60s / h. The power density of red light radiation is 20-50mW / cm 2 ; Repeat 2 to 5 times; Step three, after the soaking is completed, take out, soak in pure water for at least 24 hours, take out, wash, dry, and slice to obtain Rhodiola rosea slices.

2. The processing method according to claim 1, characterized in that: Rhodiola rosea includes Rhodiola rosea.

3. The processing method according to claim 1, characterized in that: Pure water is deionized water.

4. The processing method according to claim 1, characterized in that: Drying includes air-drying or tumble drying.

5. The Rhodiola rosea decoction piece obtained according to the processing method described in any one of claims 1 to 4.

6. The Rhodiola rosea decoction piece according to claim 5, characterized in that The content of salidroside is ≥4.85%, and the content of salidroside is ≥1.01%.

7. Use of the processing method according to any one of claims 1 to 4 in preparing Rhodiola rosea decoction pieces with high content of salidroside and high content of salidroside.

8. Use of the Rhodiola rosea slices according to claim 5 in the preparation of drugs for resisting altitude sickness, resisting fatigue and treating Alzheimer's disease.

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