High-degradation microbial deodorizing and degrading microbial agent

Abstract

The invention provides a high-degradation microbial deodorization and degradation bacterial agent, and relates to the technical field of microbial degradation. The invention relates to a high-degradation microbial deodorization degradation bacterial agent which comprises microbial strains, a carbon source, a pH regulator, a surfactant, titanate modified hydrogen peroxide and nano titanium dioxide, and the microbial strains comprise nitrifying bacteria, denitrifying bacteria, acidophilic bacteria and alkaliphilic bacteria. The compound microbial deodorization and degradation microbial agent can reduce the pollution of peculiar smell substances and organic matters in wastewater / garbage. The microbial strains enhance the conversion and degradation process of peculiar smell substances through mutual synergistic effect, and substances for promoting oxidation reaction are added, so that the oxygenolysis efficiency is improved, and the deodorization effect is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of microbial degradation, and particularly relates to a high-degradation microbial deodorizing and degrading bacterium agent. Background Art

[0002] The composite microbial deodorizing and degrading bacterium agent is a treatment method that utilizes the synergistic effect of multiple microbial strains to degrade odor substances and organic pollutants in wastewater.

[0003] Microbial strains decompose organic substances in wastewater into harmless substances through metabolic actions, or convert organic substances into odorless compounds through metabolic actions. Different microbial strains have different degradation mechanisms and degradation pathways. For example, nitrifying bacteria can convert ammonia nitrogen into nitrate, and acidophilic bacteria can convert organic substances into organic acids.

[0004] According to the composition and properties of the target wastewater, suitable microbial strains are selected to maximize the degradation effect of the bacterium agent on odor substances and organic pollutants in the wastewater. The selection of strains can be carried out through methods such as literature investigation, experimental screening, and gene analysis.

[0005] The selected microbial strains are cultured and amplified through a liquid medium or a solid medium. The culture conditions include appropriate temperature, pH value, oxygen supply, and nutrients, etc., to promote the growth and metabolic activity of the strains.

[0006] The cultured microbial strains are mixed, and appropriate amounts of carbon sources, nutrients, and other regulators are added to form a composite microbial deodorizing and degrading bacterium agent. The optimization of the bacterium agent formula needs to be adjusted according to the characteristics of the wastewater and the characteristics of the strains to improve the degradation effect and stability of the bacterium agent.

[0007] The prepared bacterium agent is applied to the target wastewater and is put into the wastewater treatment system by means of injection, spraying, or mixing, etc. The bacterium agent acts on the odor substances and organic pollutants in the wastewater for degradation and conversion.

[0008] By monitoring parameters such as the change in the concentration of target pollutants in the wastewater, the reduction degree of odor substances, and the wastewater treatment effect, etc., the effect and performance of the bacterium agent are evaluated.

[0009] The composite microbial deodorizing and degrading bacterium agent is a sustainable and environmentally friendly wastewater treatment technology with high degradation efficiency and adaptability. In terms of the preparation method, by reasonably selecting strains, optimizing the culture conditions and formula, the degradation effect of the bacterium agent can be improved. However, there are still challenges in aspects such as the stability of the bacterium agent, long-term effect, and engineering application, etc., and further research and improvement are needed.

[0010] The microbial deodorizing and degrading technology in the prior art has poor degradation effect, high price, and poor survival ability of strains. Summary of the Invention

[0011] In view of the above problems, the present invention provides a highly degradable microbial deodorizing and degradation bactericide to solve the technical problems of poor degradation effect, high price, and poor strain survival ability of microbial deodorizing and degradation bactericides in the prior art.

[0012] To achieve the above object, the present invention provides the following technical solutions:

[0013] A highly degradable microbial deodorizing and degradation bactericide, comprising microbial strains, a carbon source, a pH regulator, a surfactant, titanate-modified hydrogen peroxide, and nano-titanium dioxide. The microbial strains include nitrifying bacteria, denitrifying bacteria, acidophilic bacteria, and alkaliphilic bacteria.

[0014] Preferably, the carbon source is one or more of starches, proteins, fats, and sugars.

[0015] Preferably, the proteins include soybean meal and yeast powder.

[0016] Preferably, the surfactant is one of sodium dodecylbenzenesulfonate and alkylbenzenesulfonate.

[0017] Preferably, the composite microbial deodorizing and degradation bactericide further includes a trace element additive.

[0018] Preferably, the trace element additive is one or two of magnesium sulfate, ferric chloride, and diammonium hydrogen phosphate.

[0019] Preferably, the addition amount of each of the microbial strains is: nitrifying bacteria: 10^6 CFU / mL, denitrifying bacteria: 10^6 CFU / mL, acidophilic bacteria: 10^6 CFU / mL, alkaliphilic bacteria: 10^6 CFU / mL.

[0020] Preferably, the addition amount of the titanate-modified hydrogen peroxide is 2-5% of the total amount of the highly degradable microbial deodorizing and degradation bactericide.

[0021] Preferably, the addition amount of the nano-titanium dioxide is 4-8% of the total amount of the highly degradable microbial deodorizing and degradation bactericide.

[0022] Microbial strains:

[0023] Nitrifying bacteria: Nitrosomonas spp. can convert ammonia nitrogen in wastewater into nitrite, thereby reducing odor generation.

[0024] Denitrifying bacteria: Denitrifying bacteria spp. can convert nitrite in wastewater into nitrogen, further reducing odor and organic matter pollution.

[0025] Acidophilic bacteria: Lactobacillus spp. can decompose organic acids and reduce acidic odors.

[0026] Alkaliphilic bacteria: Alkaliphilus spp. can decompose fatty acids and reduce putrid odors.

[0027] Starches, proteins, fats, sugars, etc. provide the carbon sources and nutrients required for the growth of microorganisms. Microorganisms metabolize these organic substances to degrade the organic and odoriferous substances in wastewater.

[0028] pH regulator: Adjust according to the pH value of the wastewater to provide a suitable growth environment for the bacterial strains. For example, sodium hydroxide can be used to adjust alkaline wastewater.

[0029] Trace element additive: Provide the nutrient elements required for the growth and metabolism of microorganisms. Microorganisms require certain trace elements to maintain their normal metabolic activities during the biodegradation process.

[0030] Surfactant: Improve the penetrability and degradation efficiency of the bacterial agent, enabling microorganisms to better contact and degrade the organic substances in wastewater.

[0031] In the present invention, the addition of nano-titanium dioxide (TiO2) has a large specific surface area and a special electronic structure, and can generate strong oxidizing properties through photocatalytic effects to accelerate the degradation of organic substances. Adding titanate-modified hydrogen peroxide can improve its oxidation effect by changing the properties of hydrogen peroxide and enhance the degradation ability of aerobic bacteria.

[0032] Apply the prepared composite microbial deodorizing and degrading bacterial agent to wastewater containing odors and organic substances. The microbial strains utilize the provided carbon sources and nutrients for growth and metabolic activities. Through the processes of degrading organic substances and converting nitrogen substances, these strains reduce the odoriferous substances and organic pollution in the wastewater / garbage, and ultimately achieve the effects of deodorization and degradation. The microbial strains strengthen the process of converting and degrading odoriferous substances through mutual synergy to improve the deodorization effect. Add substances that promote oxidation and decomposition to achieve high-efficiency degradation. Detailed implementation methods

[0033] To make the objectives, technical solutions, and advantages of the present invention clearer and more understandable, the present invention will be further described in detail below. However, it should be understood that the description herein is only used to explain the present invention and is not used to limit the scope of the present invention.

[0034] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the technical field to which this invention belongs. The terms used in the description of this invention herein are for the purpose of describing specific embodiments only and are not intended to limit the invention. The reagents and instruments used herein are commercially available, and the characterization means involved can be referred to the relevant descriptions in the prior art and will not be elaborated herein.

[0035] To further understand this invention, the following provides a more detailed description of the invention in conjunction with the best embodiments.

[0036] Example 1

[0037] A highly degradable microbial deodorant degradation bactericide, comprising microbial strains, a carbon source, a pH regulator, a surfactant, titanate-modified hydrogen peroxide, and nano-titanium dioxide. The microbial strains include nitrifying bacteria, denitrifying bacteria, acidophilic bacteria, and alkaliphilic bacteria.

[0038] The carbon source is starch-based and protein-based. The protein-based includes soybean meal and yeast powder. The surfactant is sodium dodecylbenzenesulfonate. The trace element additive is magnesium sulfate and ferric chloride. The addition amount of each of the microbial strains is: nitrifying bacteria: 10^6 CFU / mL, denitrifying bacteria: 10^6 CFU / mL, acidophilic bacteria: 10^6 CFU / mL, alkaliphilic bacteria: 10^6 CFU / mL. The addition amount of the titanate-modified hydrogen peroxide is 2% of the total amount of the highly degradable microbial deodorant degradation bactericide. The addition amount of the nano-titanium dioxide is 4% of the total amount of the highly degradable microbial deodorant degradation bactericide.

[0039] A preparation method of a highly degradable microbial deodorant degradation bactericide based on the one described in this example, comprising the following steps;

[0040] S1. Cultivate nitrifying bacteria, denitrifying bacteria, acidophilic bacteria, and alkaliphilic bacteria respectively;

[0041] S2. Separate the microbial cells from the culture medium by centrifugation and filtration, and measure the activity and concentration of the collected microbial cells to obtain the microbial cells;

[0042] S3. Add the carbon source, pH regulator, and surfactant to the microbial cells and stir well to obtain a composite microbial deodorant degradation bactericide.

[0043] Example 2

[0044] A highly degradable microbial deodorant degradation bactericide, comprising microbial strains, a carbon source, a pH regulator, a surfactant, titanate-modified hydrogen peroxide, and nano-titanium dioxide. The microbial strains include nitrifying bacteria, denitrifying bacteria, acidophilic bacteria, and alkaliphilic bacteria.

[0045] The carbon source is starch, protein, fat, and sugar. The protein includes soybean meal and yeast powder. The surfactant is sodium dodecylbenzenesulfonate and alkylbenzenesulfonate. The composite microbial deodorant and degradation bactericide also includes a trace element additive, and the trace element additive is diammonium hydrogen phosphate.

[0046] The addition amount of each of the microbial strains is as follows: nitrifying bacteria: 10^6 CFU / mL, denitrifying bacteria: 10^6 CFU / mL, acidophilic bacteria: 10^6 CFU / mL, alkaliphilic bacteria: 10^6 CFU / mL.

[0047] The addition amount of the titanate-modified hydrogen peroxide is 2-5% of the total amount of the high-degradation microbial deodorant and degradation bactericide. The addition amount of the nano-titanium dioxide is 4-8% of the total amount of the high-degradation microbial deodorant and degradation bactericide.

[0048] A preparation method of a high-degradation microbial deodorant and degradation bactericide based on the one described in this embodiment includes the following steps;

[0049] S1. Cultivate nitrifying bacteria, denitrifying bacteria, acidophilic bacteria, and alkaliphilic bacteria respectively;

[0050] S2. Separate the microbial cells from the culture medium by centrifugation and filtration, and measure the activity and concentration of the collected microbial cells to obtain the microbial cells;

[0051] S3. Add the carbon source, pH regulator, and surfactant to the microbial cells and stir well to obtain the composite microbial deodorant and degradation bactericide.

[0052] Example 3

[0053] A high-degradation microbial deodorant and degradation bactericide includes microbial strains, a carbon source, a pH regulator, a surfactant, titanate-modified hydrogen peroxide, and nano-titanium dioxide. The microbial strains include nitrifying bacteria, denitrifying bacteria, acidophilic bacteria, and alkaliphilic bacteria.

[0054] The carbon source is starch, protein, fat, and sugar. The protein includes soybean meal and yeast powder. The surfactant is sodium dodecylbenzenesulfonate and alkylbenzenesulfonate. The composite microbial deodorant and degradation bactericide also includes a trace element additive, and the trace element additive is diammonium hydrogen phosphate.

[0055] The addition amount of each of the microbial strains is as follows: nitrifying bacteria: 10^6 CFU / mL, denitrifying bacteria: 10^6 CFU / mL, acidophilic bacteria: 10^6 CFU / mL, alkaliphilic bacteria: 10^6 CFU / mL.

[0056] The addition amount of the titanate-modified hydrogen peroxide is 3% of the total amount of the high-degradation microbial deodorant degradation bactericide. The addition amount of the nano-titanium dioxide is 5% of the total amount of the high-degradation microbial deodorant degradation bactericide.

[0057] Apply this composite microorganism to the deodorization treatment of the landfill waste. Adopt the method of expanding the cultivation of the composite microorganism, and perfusion degradation and spray atomization lysis adsorption treatment of the garbage heap body at a dosage of two-thousandths. After 20 days of treatment, the odor of the surrounding environment has been improved well. The removal rates of the main project indicators are as follows in the table;

[0058] Removal rate of Example 1 Removal rate of Example 2 Removal rate of Example 3 <![CDATA[Ammonia (mg / m 3 )]]> 86.5％ 83.5％ 84.5％ <![CDATA[Hydrogen sulfide (mg / m 3 )]]> 89.3％ 90.5％ 91.3％ <![CDATA[Sulfur dioxide (mg / m 3 )]]> 93.1％ 92.5％ 93.5％

[0059] It is only a preferred embodiment of the invention, and is not used to limit the present invention. The technical solutions of the present invention include, but are not limited to, each embodiment, test example or any combination of each embodiment and test example of the present invention. Any modification, equivalent replacement or improvement made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A highly degradable microbial deodorant degradation bactericide, characterized in that, it includes microbial strains, carbon sources, pH regulators, surfactants, titanate-modified hydrogen peroxide, and nano-titanium dioxide. The microbial strains include nitrifying bacteria, denitrifying bacteria, acidophilic bacteria, and alkaliphilic bacteria.

2. The highly degradable microbial deodorant degradation bactericide according to claim 1, characterized in that, the carbon source is one or more of starch, protein, fat, and sugar.

3. The highly degradable microbial deodorant degradation bactericide according to claim 2, characterized in that, the protein includes soybean meal and yeast powder.

4. The highly degradable microbial deodorant degradation bactericide according to claim 1, characterized in that, the surfactant is one of sodium dodecylbenzenesulfonate and alkylbenzenesulfonate.

5. The highly degradable microbial deodorant degradation bactericide according to claim 1, characterized in that, the composite microbial deodorant degradation bactericide further includes a trace element additive.

6. The highly degradable microbial deodorant degradation bactericide according to claim 5, characterized in that, the trace element additive is one or two of magnesium sulfate, ferric chloride, and diammonium hydrogen phosphate.

7. The highly degradable microbial deodorant degradation bactericide according to claim 1, characterized in that, the addition amount of each of the microbial strains is: nitrifying bacteria: 10^6 CFU / mL, denitrifying bacteria: 10^6 CFU / mL, acidophilic bacteria: 10^6 CFU / mL, alkaliphilic bacteria: 10^6 CFU / mL.

8. The highly degradable microbial deodorant degradation bactericide according to claim 1, characterized in that, the addition amount of the titanate-modified hydrogen peroxide is 2-5% of the total amount of the highly degradable microbial deodorant degradation bactericide.

9. The highly degradable microbial deodorant degradation bactericide according to claim 1, characterized in that, the addition amount of the nano-titanium dioxide is 4-8% of the total amount of the highly degradable microbial deodorant degradation bactericide.