Cell culture device for anti-tumor immunity

Abstract

The invention discloses a cell culture device for anti-tumor immunity, and belongs to the technical field of immune cell culture.The cell culture device comprises a culture box, a base is fixedly connected to the lower surface of the culture box, a cover plate is hinged to the upper surface of the culture box, and a motor is connected to the outer wall of the rear side of the culture box through bolts; a material box is connected to the outer wall of the front side of the culture box through bolts; a long plate is movably connected to the interior of the incubator, and a connecting plate is fixedly connected to the upper surface of the long plate. According to the cell culture device for anti-tumor immunity, when a test tube needs to be clamped, a movable plate is firstly stirred to enable a long pin to stretch a torsion spring, then the test tube is placed in a placement groove, after the movable plate is slowly loosened, the movable plate and a limiting plate are close to the test tube under the action of the torsion spring, and at the moment, the limiting plate drives a rolling ball to rotate in the movable plate; the limiting plates are always in a vertical state, finally the limiting plates on the two sides abut against and clamp the test tube, the operation process is rapid and convenient, the working efficiency is high, and the clamping effect is good.

Description

Technical Field

[0001] The present invention relates to the technical field of immune cell culture, and specifically to a cell culture device for anti-tumor immunity. Background Art

[0002] Tumor is a disease that seriously endangers the human body and poses a huge threat to human health. Among various means of combating tumors, culturing anti-tumor immune cells is an extremely important step. During the process of culturing anti-tumor immune cells, an incubator is required. The incubator is one of the important devices for anti-tumor immune cell research. When the incubator is working, it can be statically cultured or shaken. At the same time, the incubator can provide a suitable temperature and sterile environment for the culture dishes or culture test tubes in the box. When the incubator needs to perform a shaking operation, the test tubes need to be kept stable, otherwise it will affect the raw materials inside the test tubes and bring unnecessary trouble to the work of culturing anti-tumor immune cells.

[0003] In order to overcome the above defects, Prior Art 1 (a Chinese patent application with the application number CN202410001138.7 and the application date of January 2, 2024) is a tumor cell culture device. When it works, it can quickly clamp the culture container through the clamping plate, and can also select different tumor cell culture containers at different time periods according to the culture requirements, and can ensure the stability of the tube-taking action, which is convenient for users to operate. Prior Art 2 (a Chinese patent application with the application number CN202210785149.X and the application date of July 6, 2022) is a shaking mixing device for cell culture. It provides a shaking mixing device for cell culture, which can stably clamp test tubes of different specifications, can independently operate some test tubes so that they do not vibrate, can independently adjust the shaking amplitude of different test tubes, and automatically stops shaking when the test tube is detached, and the use effect is better.

[0004] During the use process, the incubator needs to be opened and closed by the staff, so the environment around the incubator is also extremely important, which may affect the result of culturing anti-tumor immune cells. The devices in the above applications do not have the function of assisting disinfection during the use process, which will affect the culturing process and result of anti-tumor immune cells, and further affect the analysis and judgment of data by researchers.

[0005] Therefore, we propose a cell culture device for anti-tumor immunity to solve the above-mentioned problems. Summary of the Invention

[0006] The purpose of the present invention is to provide a cell culture device for anti-tumor immunity, so as to solve the problem that in the process of use, it does not have the function of assisting disinfection, which will affect the culture process and results of anti-tumor immune cells, and further affect the analysis and judgment of data by scientific researchers as put forward in the above-mentioned background technology.

[0007] To achieve the above purpose, the present invention provides the following technical solutions: A cell culture device for anti-tumor immunity, including an incubator, the lower surface of the incubator is fixedly connected with a base, and the upper surface of the incubator is hingedly connected with a cover plate. Moreover, a motor is bolted to the rear outer wall of the incubator, and a material box is bolted to the front outer wall of the incubator. A long plate for placing test tubes for cell culture is movably connected inside the incubator. The output end of the motor is fixedly connected with a connecting shaft, and the end of the connecting shaft is fixedly connected with a working plate. Moreover, a shaking assembly is arranged on the surface of the working plate. The shaking assembly includes a fixed block fixedly connected to the surface of the working plate. When the shaking assembly works, it makes the long plate move in the vertical direction. An airbag is adhesively connected to the bottom of the incubator, and a disinfection assembly is arranged between the incubator and the airbag. The disinfection assembly includes a side pipe fixedly connected to the inside of the incubator, and a atomizing nozzle is fixedly connected to the end of the side pipe. When the disinfection assembly works, it can spray disinfectant around the incubator.

[0008] Preferably, a connecting plate is fixedly connected to the upper surface of the long plate, and a limiting assembly for clamping the test tube is arranged on the connecting plate. The limiting assembly includes a long pin fixedly connected to the inner wall of the connecting plate. The limiting assembly also includes a movable plate sleeved on the surface of the long pin, and a rolling ball is rotatably arranged inside the movable plate, and a limiting plate is fixedly connected to the end of the rolling ball.

[0009] Preferably, a placement groove is formed on the surface of the long plate. The limiting plates are symmetrically distributed about the center of the placement groove, and the placement grooves are equally spaced on the surface of the long plate.

[0010] Preferably, a torsion spring for elastic reset is fixedly connected to the surface of the long pin, and the other side of the torsion spring is fixedly connected to the inner wall of the movable plate.

[0011] Preferably, the shaking assembly further includes a movable frame sleeved on the surface of the fixed block, and a connecting rod is fixedly connected to the lower surface of the movable frame, and a pressing plate is fixedly connected to the lower surface of the connecting rod.

[0012] Preferably, a column is fixedly connected to the inner wall of the incubator, and the column passes through the inside of the pressing plate, and the column is slidably connected to the pressing plate, and the columns are symmetrically distributed about the center of the pressing plate.

[0013] Preferably, an upper connecting rod is fixedly connected to the upper surface of the movable frame, and the other side of the upper connecting rod is fixedly connected to the lower surface of the long board, a guide rod is fixedly connected to the inner wall of the incubator, and the guide rod passes through the interior of the long board, and the guide rod is an inverted "U" shape when viewed from the front.

[0014] Preferably, the disinfection assembly also includes a working tube fixedly connected to the surface of the airbag, and the airbag is fixedly connected to the side tube, and a first one-way valve is fixedly connected to the surface of the side tube, and the first one-way valve allows the disinfectant to flow from the airbag to the atomizing nozzle.

[0015] Preferably, a lower connecting pipe is fixedly connected to the lower surface of the material box, the end of the working pipe is located outside the incubator, and the working pipe is connected to the lower connecting pipe through an external hose.

[0016] Preferably, a second one-way valve is fixedly connected to the surface of the working tube, and the second one-way valve allows the disinfectant to flow from the material box to the airbag.

[0017] Compared with the prior art, the beneficial effects of the present invention are as follows: a new type of structural design is adopted, and the test tube can be quickly clamped and fixed by setting a movable plate and a limit plate, which has high working efficiency; an upper connecting rod and a long plate that can move in the vertical direction are also provided, so that the test tube can be swung, which plays the role of shaking table culture; in addition, a material box, an air bag and an atomizing nozzle are also provided, so that the disinfectant can be sprayed intermittently around the incubator, which plays the role of sterilization and disinfection, thereby facilitating the cultivation of anti-tumor immune cells; a first one-way valve and a second one-way valve are also provided, and when the first one-way valve and the second one-way valve are in operation, the disinfectant flows in the direction from the material box to the atomizing nozzle, and the disinfectant does not flow back, thereby ensuring the working stability of the air bag and the atomizing nozzle. The specific contents are as follows: (1) When the anti-tumor immune cell culture device is used and a test tube needs to be clamped, the movable plate is first moved to stretch the torsion spring with the long pin, and then the test tube is placed in the placement slot. After the movable plate is slowly released, the movable plate and the limit plate are moved close to the test tube under the action of the torsion spring. At this time, the limit plate drives the ball to rotate inside the movable plate, and the limit plate is always in a vertical state. Finally, the limit plates on both sides clamp the test tube. The operation process is quick and convenient, the work efficiency is high, and the clamping effect is good.

[0018] Furthermore, the limiting plates are distributed on both sides of the placement slots, and the placement slots are evenly spaced on the upper surface of the long board, so the long board can clamp multiple test tubes at the same time, ensuring the accuracy of the test data and thus improving work efficiency.

[0019] (2) When the shaker culture is needed for this anti-tumor immunity cell culture device, the motor can be started. The motor drives the working plate and the fixing block to rotate through the connecting shaft. At this time, the movable frame, the connecting rod and the pressing plate, under the cooperation of the vertical column and the fixing block, make a reciprocating linear motion in the vertical direction. At this time, the movable frame drives the long plate to make a reciprocating linear motion in the vertical direction through the upper connecting rod, so that the incubator has the function of shaker culture.

[0020] Furthermore, during the movement of the long plate, the guide rod cooperates with the vertical column, optimizing the stability of the long plate's operation.

[0021] (3) In this anti-tumor immunity cell culture device, a disinfectant is placed inside the material box. When the pressing plate descends, the pressing plate will squeeze the airbag. When the airbag is squeezed, the airbag transports the disinfectant through the side pipe. At this time, the atomizing nozzle sprays the disinfectant, optimizing the environment around the incubator.

[0022] Furthermore, when the pressing plate rises, the airbag is not squeezed. At this time, the airbag expands back under its own characteristics. When the airbag expands back, it inhales the disinfectant inside the material box into its own interior through the working pipe and the lower connecting pipe, facilitating the next operation of the airbag.

[0023] Even further, during the operation of the airbag, the side pipe, the working pipe and the atomizing nozzle, the first one-way valve and the second one-way valve make the flow direction of the disinfectant from the material box to the atomizing nozzle, preventing the reverse flow of the disinfectant and ensuring the stability of the operation of the airbag and the atomizing nozzle. Description of the Drawings

[0024] Figure 1 Schematic diagram of the connection structure between the incubator and the cover plate of the present invention; Figure 2 Schematic diagram of the connection structure between the incubator and the motor of the present invention; Figure 3 Schematic diagram of the distribution state of the placement grooves on the long plate of the present invention; Figure 4 Schematic diagram of the connection structure between the movable frame, the upper connecting rod and the long plate of the present invention; Figure 5 Schematic diagram of the connection structure between the movable plate, the rolling ball and the limiting plate of the present invention; Figure 6 Schematic diagram of the connection structure between the torsion spring, the long pin and the movable plate of the present invention; Figure 7 Schematic diagram of the connection structure between the motor and the connecting shaft of the present invention; Figure 8 Schematic diagram of the connection structure between the movable frame and the connecting rod of the present invention; Figure 9 Schematic diagram of the state when the pressing plate descends in the present invention.

[0025] In the figure: 1. Incubator; 2. Cover plate; 3. Motor; 4. Material box; 5. Lower connecting pipe; 6. Connecting shaft; 7. Working plate; 8. Fixed block; 9. Movable frame; 10. Connecting rod; 11. Pressing plate; 12. Column; 13. Connecting plate; 14. Torsion spring; 15. Long pin; 16. Movable plate; 17. Ball; 18. Limiting plate; 19. Long plate; 20. Guide rod; 21. Airbag; 22. Side pipe; 23. Atomizing nozzle; 24. First one-way valve; 25. Working pipe; 26. Second one-way valve; 27. Upper connecting rod; 28. Placing groove. Specific implementation manner

[0026] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0027] Embodiment 1: By providing the movable plate 16, the limiting plate 18 and the torsion spring 14, the required test tube can be quickly clamped and fixed, facilitating the shaking culture work of the long plate 19. As Figures 1-6 shown, it includes an incubator 1. The lower surface of the incubator 1 is fixedly connected with a base, and the upper surface of the incubator 1 is hingedly connected with a cover plate 2. Moreover, a motor 3 is bolted to the rear outer wall of the incubator 1, and a material box 4 is bolted to the front outer wall of the incubator 1. A long plate 19 is movably connected inside the incubator 1, and a connecting plate 13 is fixedly connected to the upper surface of the long plate 19. And a limiting component for clamping the test tube is arranged on the connecting plate 13. The limiting component includes a long pin 15 fixedly connected to the inner wall of the connecting plate 13.

[0028] The limiting component further includes a movable plate 16 sleeved on the surface of the long pin 15. A ball 17 is rotatably arranged inside the movable plate 16, and a limiting plate 18 is fixedly connected to the end of the ball 17. Placing grooves 28 are formed on the surface of the long plate 19. The limiting plates 18 are symmetrically distributed about the center of the placing groove 28, and the placing grooves 28 are equally spaced on the surface of the long plate 19. A torsion spring 14 for elastic reset is fixedly connected to the surface of the long pin 15, and the other side of the torsion spring 14 is fixedly connected to the inner wall of the movable plate 16.

[0029] During use, when it is necessary to clamp a test tube, the operator can first move the movable plate 16, causing the movable plate 16 to rotate around the long pin 15, increasing the distance between adjacent limit plates 18. At this time, the long pin 15 and the movable plate 16 cooperate to stretch the torsion spring 14. Then, place the test tube in the placement groove 28. After slowly releasing the movable plate 16, the movable plate 16 and the limit plate 18 rotate back under the action of the torsion spring 14 and approach the test tube. At this time, the limit plate 18 drives the rolling ball 17 to rotate inside the movable plate 16, that is, the limit plate 18 always remains in a vertical state and closely fits the outer wall of the test tube. Finally, the limit plates 18 on both sides hold and clamp the test tube. The operation process is fast and convenient, with high work efficiency and good clamping effect. The limit plates 18 are distributed on both sides of the placement groove 28, and the placement grooves 28 are equally spaced on the upper surface of the long plate 19. Therefore, the long plate 19 can clamp multiple test tubes at the same time, ensuring the accuracy of test data and thus improving work efficiency.

[0030] Embodiment 2: Different from Embodiment 1, through the provided shaking assembly, with the cooperation of the motor 3, the connecting shaft 6, and the fixing block 8, the long plate 19, the movable plate 16, and the clamped test tube can perform reciprocating linear motion in the vertical direction. At this time, the long plate 19 can shake the test tube, so that the incubator 1 has the function of shaker culture, as Figures 7-9 shown. The output end of the motor 3 is fixedly connected to the connecting shaft 6, and the end of the connecting shaft 6 is fixedly connected to the working plate 7. And a shaking assembly is arranged on the surface of the working plate 7. The shaking assembly includes a fixing block 8 fixedly connected to the surface of the working plate 7. When the shaking assembly works, it makes the long plate 19 move in the vertical direction. The shaking assembly also includes a movable frame 9 sleeved on the surface of the fixing block 8, and the lower surface of the movable frame 9 is fixedly connected to a connecting rod 10, and the lower surface of the connecting rod 10 is fixedly connected to a pressing plate 11.

[0031] A column 12 is fixedly connected to the inner wall of the incubator 1, and the column 12 passes through the inside of the pressing plate 11, and the column 12 is slidably connected to the pressing plate 11, and the columns 12 are symmetrically distributed about the center of the pressing plate 11. The upper surface of the movable frame 9 is fixedly connected to an upper connecting rod 27, and the other side of the upper connecting rod 27 is fixedly connected to the lower surface of the long plate 19. A guide rod 20 is fixedly connected to the inner wall of the incubator 1, and the guide rod 20 passes through the inside of the long plate 19, and the front view of the guide rod 20 is an inverted "U" shape.

[0032] When the shaker culture is required, the motor 3 can be started. The motor 3 drives the working plate 7 and the fixing block 8 to rotate through the connecting shaft 6. At this time, the movable frame 9, the connecting rod 10 and the pressing plate 11 make a reciprocating linear motion in the vertical direction under the cooperation of the column 12 and the fixing block 8. At this time, the movable frame 9 drives the long plate 19 to make a reciprocating linear motion in the vertical direction through the upper connecting rod 27. Then the test tube is in a shaking state, and thus the incubator 1 has the function of shaker culture. During the movement of the long plate 19, the guide rod 20 cooperates with the column 12 to optimize the working stability of the long plate 19.

[0033] Embodiment 3: Different from Embodiment 2, through the arranged disinfection component, the airbag 21 and the atomizing nozzle 23 can work. The atomizing nozzle 23 sprays disinfectant solution, which can disinfect the air around the incubator 1, ensuring the safety of the working environment of the incubator 1, and thus ensuring the stability of the culture result. As Figure 7 and Figure 9 shown, the airbag 21 is adhesively connected to the bottom of the incubator 1, and a disinfection component is arranged between the incubator 1 and the airbag 21. The disinfection component includes a side pipe 22 fixedly connected to the inside of the incubator 1, and an atomizing nozzle 23 is fixedly connected to the end of the side pipe 22. When the disinfection component works, it can spray disinfectant solution around the incubator 1. The disinfection component further includes a working pipe 25 fixedly connected to the surface of the airbag 21, and the airbag 21 is fixedly connected to the side pipe 22. And a first one-way valve 24 is fixedly connected to the surface of the side pipe 22, and the first one-way valve 24 makes the flow direction of the disinfectant solution be from the airbag 21 to the atomizing nozzle 23.

[0034] The lower surface of the material box 4 is fixedly connected with a lower connecting pipe 5. The end of the working pipe 25 is located outside the incubator 1, and the working pipe 25 is connected to the lower connecting pipe 5 through an external hose. A second one-way valve 26 is fixedly connected to the surface of the working pipe 25, and the second one-way valve 26 makes the flow direction of the disinfectant solution be from the material box 4 to the airbag 21.

[0035] When the pressing plate 11 makes a reciprocating linear motion in the vertical direction, it will intermittently squeeze the airbag 21. When the pressing plate 11 descends, the airbag 21 will be squeezed. At this time, the airbag 21 will transport the disinfectant to the atomizing nozzle 23 through the side pipe 22, and finally the atomizing nozzle 23 will spray the disinfectant. When the pressing plate 11 ascends, it will no longer squeeze the airbag 21. At this time, the airbag 21 will expand under the action of its own characteristics. When the airbag 21 expands, it will absorb the disinfectant through the working pipe 25 and the lower connecting pipe 5, so that the disinfectant enters the airbag 21 from the inside of the material box 4. Then, during the process of the pressing plate 11 making a reciprocating linear motion, the airbag 21 will absorb the disinfectant and then discharge the disinfectant through the atomizing nozzle 23. At this time, the atomizing nozzle 23 and the airbag 21 play a role in purifying the air around the incubator 1, improving safety and making the culture result better. When the atomizing nozzle 23 and the airbag 21 are working, the first one-way valve 24 and the second one-way valve 26 make the working direction of the disinfectant from the material box 4 to the atomizing nozzle 23, and the situation of the disinfectant flowing back will not occur, ensuring the stability of the work of the atomizing nozzle 23 and the airbag 21.

[0036] The above is the working process of the entire device, and the content not described in detail in this specification belongs to the prior art well-known to those skilled in the art.

Claims

1. A cell culture device for anti-tumor immunity, comprising a culture box (1), wherein a base is fixedly connected to the lower surface of the culture box (1), and a cover plate (2) is hingedly connected to the upper surface of the culture box (1), and a motor (3) is bolted to the rear outer wall of the culture box (1), and a material box (4) is bolted to the front outer wall of the culture box (1); Features: The incubator (1) is movably connected inside with a long plate (19) for placing test tubes for cell culture; The output end of the motor (3) is fixedly connected to a connecting shaft (6), and the end of the connecting shaft (6) is fixedly connected to a working plate (7), and a shaking assembly is provided on the surface of the working plate (7), the shaking assembly comprising a fixed block (8) fixedly connected to the surface of the working plate (7), and when the shaking assembly is in operation, the long plate (19) moves in a vertical direction; The bottom of the incubator (1) is bonded with an air bag (21), and a disinfection assembly is provided between the incubator (1) and the air bag (21). The disinfection assembly comprises a side tube (22) fixedly connected to the inside of the incubator (1), and an atomizing nozzle (23) is fixedly connected to the end of the side tube (22). When the disinfection assembly is in operation, disinfectant is sprayed around the incubator (1).

2. The anti-tumor immune cell culture device according to claim 1, characterized in that: The upper surface of the long plate (19) is fixedly connected to a connecting plate (13), and a limit assembly for clamping the test tube is provided on the connecting plate (13), the limit assembly comprising a long pin (15) fixedly connected to the inner wall of the connecting plate (13), the limit assembly also comprising a movable plate (16) sleeved and connected to the surface of the long pin (15), a rolling ball (17) being rotatably provided inside the movable plate (16), and the end of the rolling ball (17) being fixedly connected to the limit plate (18).

3. The anti-tumor immune cell culture device according to claim 2, characterized in that: The surface of the long plate (19) is provided with a placement groove (28), the limiting plates (18) are symmetrically distributed about the center of the placement groove (28), and the placement grooves (28) are evenly spaced on the surface of the long plate (19).

4. The anti-tumor immune cell culture device according to claim 2, characterized in that: A torsion spring (14) having an elastic reset function is fixedly connected to the surface of the long pin (15), and the other side of the torsion spring (14) is fixedly connected to the inner wall of the movable plate (16).

5. The anti-tumor immune cell culture device according to claim 1, characterized in that: The shaking assembly further comprises a movable frame (9) sleeved and connected to the surface of the fixed block (8), wherein the lower surface of the movable frame (9) is fixedly connected to a connecting rod (10), and the lower surface of the connecting rod (10) is fixedly connected to a pressing plate (11).

6. The anti-tumor immune cell culture device according to claim 5, characterized in that: A column (12) is fixedly connected to the inner wall of the incubator (1), and the column (12) passes through the interior of the pressing plate (11). The column (12) and the pressing plate (11) are slidably connected, and the columns (12) are symmetrically distributed about the center of the pressing plate (11).

7. The anti-tumor immune cell culture device according to claim 5, characterized in that: An upper connecting rod (27) is fixedly connected to the upper surface of the movable frame (9), and the other side of the upper connecting rod (27) is fixedly connected to the lower surface of the long board (19). A guide rod (20) is fixedly connected to the inner wall of the incubator (1), and the guide rod (20) passes through the interior of the long board (19), and the guide rod (20) is an inverted "U" shape when viewed from the front.

8. The anti-tumor immune cell culture device according to claim 1, characterized in that: The disinfection assembly further comprises a working tube (25) fixedly connected to the surface of the airbag (21), the airbag (21) and the side tube (22) are fixedly connected, and a first one-way valve (24) is fixedly connected to the surface of the side tube (22), and the first one-way valve (24) allows the disinfectant to flow from the airbag (21) to the atomizing nozzle (23).

9. The anti-tumor immune cell culture device according to claim 8, characterized in that: A lower connecting pipe (5) is fixedly connected to the lower surface of the material box (4), the end of the working pipe (25) is located outside the incubator (1), and the working pipe (25) is connected to the lower connecting pipe (5) via an external hose.

10. The anti-tumor immune cell culture device according to claim 8, characterized in that: A second one-way valve (26) is fixedly connected to the surface of the working tube (25), and the second one-way valve (26) allows the disinfectant to flow from the material box (4) to the air bag (21).

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