Natural-source instant wrinkle-removing composition and application thereof, cosmetic and preparation method thereof

Abstract

The invention discloses a natural-source instant wrinkle-removing composition and application thereof, a cosmetic and a preparation method of the cosmetic. The natural-source instant wrinkle-removing composition is prepared from the following components in parts by weight: 0.1 to 6 parts of apple extract, 1 to 5 parts of peach resin extract and 0.01 to 0.5 part of hydroxyproline. The components in the composition are all natural products, and the composition with a good instant wrinkle removing effect can be obtained by matching the components. Meanwhile, compared with an existing wrinkle removing mode, when the natural-source instant wrinkle removing composition is used, the burden of skin can be effectively reduced on the basis that a good wrinkle removing effect is achieved, and the natural-source instant wrinkle removing composition is more friendly to the skin.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to a natural-source instant wrinkle-removing composition and its application, a cosmetic, and a preparation method thereof. Background Art

[0002] In the cosmetics market, as people's attention to skin aging problems continues to rise, the demand for products with wrinkle-removing and anti-wrinkle effects is showing a continuous growth trend. However, at present, the ingredients that play a role in cosmetics with such effects often require consumers to use them for a long time and periodically to see obvious effects. During the use process, consumers cannot significantly feel the improvement of the skin condition in a short time, which not only leads to a poor user experience but also greatly weakens the attractiveness of the product to consumers. After all, in modern fast-paced life, consumers expect to use products that can instantly provide firming and wrinkle-removing effects so as to quickly modify their appearance in some sudden social, business and other scenarios.

[0003] Currently, the common instant wrinkle-removing products on the market can be mainly divided into two categories. One category is toxin-like products, such as syn-ake and acetyl hexapeptide-8. Taking syn-ake as an example, it is a synthetic small-molecule peptide compound, also called snake venom tripeptide, and its mechanism of action is similar to that of botulinum toxin, and it can mimic the nerve paralysis and muscle contraction characteristics produced after the venom of poisonous snakes invades the human body. Specifically, syn-ake can bind to the N-type acetylcholine receptor on muscle cells, effectively blocking the signal transmission of muscle contraction, making the muscles in a relaxed state, thereby reducing the generation of wrinkles and achieving the purpose of anti-wrinkle and anti-aging. It can be used to smooth various expression lines, static lines and fine lines, and smooth the skin. However, this type of product has certain limitations. Its effect of inhibiting nerve conduction may have potential adverse effects on the nervous system. Some people may be allergic to syn-ake, and after use, there will be discomfort symptoms such as skin redness and itching. Long-term use may cause some unforeseen health problems. The other category is to achieve instant wrinkle-removing effects by adding filling ingredients such as silicone elastomers and using a pseudo-filling mechanism. The silicone elastomer microparticles can fill the depressions on the skin surface, achieving a filling effect and making wrinkles and pores instantly invisible. But this method also has obvious drawbacks. For example, the filling ingredients are likely to cause the skin to be unable to breathe normally, thereby triggering a series of skin problems, such as pore clogging, acne growth, skin allergy, etc. Excessive use also has the risk of causing acne and clogged pores, which instead damages skin health.

[0004] Therefore, it is necessary to further improve wrinkle-removing products. Summary of the Invention

[0005] Based on the above problems, the present invention provides a natural-source instant wrinkle-removing composition. The raw materials of the natural-source instant wrinkle-removing composition are natural products, and through the selection and synergistic cooperation of natural products, the composition can obtain good instant wrinkle-removing effects.

[0006] To achieve the above object, the present invention adopts the following technical solutions:

[0007] A natural-source instant wrinkle-removing composition, comprising the following components in parts by weight: 0.1 part - 6 parts of apple extract, 1 part - 5 parts of peach resin extract, and 0.01 part - 0.5 part of hydroxyproline.

[0008] The application of the natural-source instant wrinkle-removing composition in the preparation of instant wrinkle-removing cosmetics.

[0009] Optionally, the instant wrinkle removal includes instant reduction of the area of wrinkles.

[0010] Optionally, the wrinkles include at least one of nasolabial folds, forehead wrinkles, crow's feet, and canthus wrinkles.

[0011] An instant wrinkle-removing cosmetic, comprising the natural-source instant wrinkle-removing composition.

[0012] Optionally, the mass percentage of the natural-source instant wrinkle-removing composition in the instant wrinkle-removing cosmetic is 2% - 20%.

[0013] Optionally, the instant wrinkle-removing cosmetic comprises the following components in mass percentages: 2% - 20% of the natural-source instant wrinkle-removing composition, 0.1% - 2% of a preservative, and the balance being water.

[0014] Optionally, the instant wrinkle-removing cosmetic comprises the following components in mass percentages: 2% - 20% of the natural-source instant wrinkle-removing composition, 2% - 5% of glycerol, 2% - 5% of 1,3-propanediol, 0.01% - 0.05% of xanthan gum, 0.01% - 0.05% of hyaluronic acid, 0.2% - 0.4% of acrylate / C10-30 alkyl acrylate cross-linked polymer, 0.1% - 0.5% of p-hydroxyacetophenone, 0.02% - 0.1% of L-arginine, 0.1 - 0.5% of hexylene glycol, and the balance being water.

[0015] The preparation method of the instant wrinkle-removing cosmetic, comprising the following steps: mixing the components evenly and performing a homogenization treatment at 65°C - 75°C, then cooling and filtering to obtain the instant wrinkle-removing cosmetic.

[0016] Optionally, the preparation of the apple extract comprises the following steps: peeling, coring and dicing the apples; subjecting them to vacuum freeze-drying treatment at -40°C and 20 Pa until the water content is ≤3%, and then pulverizing to obtain freeze-dried powder with a particle size of 80 mesh; mixing the freeze-dried powder with pure water at a mass ratio of 1:15, and performing pulsed microwave treatment with a power of 600 W, a frequency of 2450 MHz, and a pulse interval of 5 s to obtain a pre-product; placing the pre-product in a supercritical fluid extraction kettle, using carbon dioxide as the supercritical fluid, at 35 MPa and 45°C, with an ethanol aqueous solution with a volume concentration of 10% as the entrainer, dynamically extracting for 60 min to extract the fat-soluble components containing spherical collagen; adjusting the system to 25 MPa and 55°C, switching the entrainer to a citric acid aqueous solution with a mass concentration of 5%, dynamically extracting for 60 min to extract the linear apple fibers; separating spherical collagen and linear apple fibers by molecular sieve chromatography; then performing fractional purification by gradient ethanol precipitation, and the volume concentrations of ethanol in the gradient ethanol precipitation are 40%, 60%, and 80% in sequence; finally, obtaining a white crystalline powder with a water content ≤3% by vacuum freeze-drying.

[0017] The preparation of the peach resin extract comprises the following steps: drying the peach resin until the water content is below 10%, quickly freezing with liquid nitrogen, and pulverizing the material to a particle size of 120 mesh to obtain an active powder with a specific surface area ≥3.5 m 2 / g; adding pure water to the active powder according to a liquid-solid ratio of 1:10 - 1:15, and performing gradient temperature-variable extraction. The conditions of the gradient temperature-variable extraction are: leaching with pure water at 4°C for 2 h, leaching with phosphate buffer solution at 25°C for 1.5 h, and leaching with an ethanol-aqueous solution containing 0.1% hyaluronidase by mass percentage at 40°C for 1 h. The volume concentration of ethanol in the ethanol-aqueous solution is 30% to obtain an extraction product; centrifuging the extraction product, fractionating through a 10 kDa ultrafiltration membrane, and separately collecting the first polysaccharide component with a molecular weight >50 kDa and the second polysaccharide component with a molecular weight <10 kDa; fractionally retaining through a membrane separation system of 100 kDa and 30 kDa ultrafiltration membranes, precipitating the first polysaccharide with an ethanol aqueous solution with a volume concentration of 60%, precipitating the second polysaccharide with an ethanol aqueous solution with a volume concentration of 80%, and finally performing low-temperature vacuum concentration under the conditions of 45°C and 0.08 MPa; then performing spray drying to obtain the peach resin extract, with an inlet air temperature of 110°C and an outlet air temperature of 55°C for spray drying.

[0018] Beneficial effects

[0019] The instant anti-wrinkle composition of natural origin of the present invention comprises apple extract in an amount of 0.1 part - 6 parts by weight, peach resin extract in an amount of 1 part - 5 parts by weight, and hydroxyproline in an amount of 0.01 part - 0.5 parts by weight. These components are all natural products. By using these components in combination, a composition with good instant anti-wrinkle effect can be obtained. At the same time, compared with the existing anti-wrinkle methods, when using the instant anti-wrinkle composition of natural origin of the present invention, on the basis of obtaining a good anti-wrinkle effect, it can effectively reduce the burden on the skin and is more friendly to the skin. Brief Description of the Drawings

[0020] Figure 1 It is a comparison chart of the area of crow's feet before and after the test subject used the essence in Example 1 of the present invention.

[0021] Figure 2 It is a curve graph showing the change of the reduction rate of the area of crow's feet corresponding to the essence in Example 1 of the present invention over time.

[0022] Figure 3 It is a test result graph of the irritation of the composition in Example 1 of the present invention. Detailed Description of the Invention

[0023] An embodiment of the present invention provides an instant anti-wrinkle composition of natural origin. The instant anti-wrinkle composition of natural origin comprises the following components in parts by weight: 0.1 part - 6 parts of apple extract, 1 part - 5 parts of peach resin extract, and 0.01 part - 0.5 parts of hydroxyproline. These components are all natural products. By using these components in combination, a composition with good instant anti-wrinkle effect can be obtained.

[0024] During the exploration process of the anti-wrinkle composition by the inventors of the present invention, it was found that apple extract, peach resin extract, hydroxyproline, and their synergistic combination have the following effects:

[0025] Apple extract can achieve physical filling anti-wrinkle and stretching and firming: Apple extract contains unique spherical plant collagen and linear apple fibers. The spherical plant collagen has high affinity for the skin and can be filled into the wrinkles of the skin to smooth out fine textures and reduce wrinkles visually. The linear apple fibers have good binding with the spherical plant collagen to form a unique three-dimensional grid-like structure with a very excellent shrinkage ratio. After the two act together, they can tighten the skin, thus providing instant anti-wrinkle and firming effects, enhancing the elasticity and toughness of the skin.

[0026] Peach resin extract (which can also be called peach gum) can achieve antioxidant effects and inhibit elastase: Peach resin extract contains high concentrations of small-molecule mucopolysaccharides and polyphenolic substances, which can act as electron donors to convert reactive free radicals into more stable products, thus achieving antioxidant effects; and it can inhibit the degradation of elastin by elastase (including dermal fibroblast elastase, neutrophil elastase, etc., fibronectin, laminin, type I and type III collagen, etc.).

[0027] Hydroxyproline can promote the synthesis of collagen and elastin and scavenge free radicals: Hydroxyproline is an important component of collagen and elastin, which can stimulate fibroblasts to synthesize protein substances, thereby achieving the restoration of skin elasticity; the hydroxyl group on the pyrrolidone ring of hydroxyproline will react with the hydroxyl free radicals in the skin, which can reduce the oxidation of free radicals to the skin.

[0028] The synergistic combination of apple extract, peach resin extract, and hydroxyproline can achieve good immediate anti-wrinkle effects.

[0029] Furthermore, the natural-source immediate anti-wrinkle composition consists of the following components in parts by weight: 0.1 part - 6 parts of apple extract, 1 part - 5 parts of peach resin extract, and 0.01 part - 0.5 part of hydroxyproline.

[0030] Optionally, in the natural-source immediate anti-wrinkle composition, the parts by weight of the apple extract can be 0.1 part, 0.5 part, 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, 5.5 parts, 6 parts. It can be understood that the parts by weight of the apple extract in the natural-source immediate anti-wrinkle composition can also make other suitable selections within the range of 0.1 part - 6 parts.

[0031] Optionally, in the natural-source immediate anti-wrinkle composition, the parts by weight of the peach resin extract can be 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts. It can be understood that the parts by weight of the peach resin extract in the natural-source immediate anti-wrinkle composition can also make other suitable selections within the range of 1 part - 5 parts.

[0032] Optionally, in the natural-source immediate anti-wrinkle composition, the parts by weight of the hydroxyproline can be 0.01 part, 0.05 part, 0.1 part, 0.15 part, 0.2 part, 0.25 part, 0.3 part, 0.35 part, 0.4 part, 0.45 part, 0.5 part. It can be understood that the parts by weight of the hydroxyproline in the natural-source immediate anti-wrinkle composition can also make other suitable selections within the range of 0.01 part - 0.5 part.

[0033] It is understandable that instant wrinkle removal means that wrinkles can be quickly removed. For example, good wrinkle removal effects can be achieved within 15 minutes. For another example, by using the instant wrinkle removal composition of the present invention, good wrinkle removal effects can be obtained within 15 minutes after use.

[0034] Another embodiment of the present invention provides an application of the above-mentioned instant wrinkle removal composition from natural sources in the preparation of instant wrinkle removal cosmetics.

[0035] It is understandable that instant wrinkle removal includes instant reduction of the area of wrinkles.

[0036] Optionally, the wrinkles include at least one of nasolabial folds, forehead wrinkles, crow's feet, and canthus wrinkles.

[0037] Another embodiment of the present invention provides an instant wrinkle removal cosmetic. The instant wrinkle removal cosmetic includes the above-mentioned instant wrinkle removal composition from natural sources.

[0038] In some embodiments, the mass percentage of the instant wrinkle removal composition from natural sources in the instant wrinkle removal cosmetic is 2% - 20%. In the instant wrinkle removal cosmetic, if the mass percentage of the instant wrinkle removal composition is too small, it is difficult to fully exhibit the instant wrinkle removal effect of the cosmetic, while if the mass percentage of the instant wrinkle removal composition in the instant wrinkle removal cosmetic is too large, it is also difficult to further significantly improve the wrinkle removal effect of the cosmetic, but instead will increase the manufacturing cost of the cosmetic. That is, in the cosmetic, when the mass percentage of the instant wrinkle removal composition from natural sources is within the range of 2% - 20%, the cosmetic can balance good instant wrinkle removal effects and relatively low manufacturing costs.

[0039] Optionally, the mass percentage of the instant wrinkle removal composition from natural sources in the instant wrinkle removal cosmetic is 2%, 5%, 8%, 10%, 12%, 15%, 18%, 20%. It is understandable that the mass percentage of the instant wrinkle removal composition from natural sources in the instant wrinkle removal cosmetic can also be other suitable choices within the range of 2% - 20%.

[0040] In some embodiments, the instant wrinkle removal cosmetic includes the following components in mass percentages: 2% - 20% of the instant wrinkle removal composition from natural sources, 0.1% - 2% of a preservative, and the balance being water. Optionally, the mass percentage of the preservative can be 0.1%, 0.2%, 0.5%, 0.8%, 1%, 1.2%, 1.5%, 1.8%, 2%, etc. It is understandable that the mass percentage of the preservative can also be other suitable choices within the range of 0.1% - 2%.

[0041] In some embodiments, the instant wrinkle removal cosmetic consists of the following components in mass percentages: 2% - 20% of the instant wrinkle removal composition from natural sources, 0.1% - 2% of a preservative, and the balance being water.

[0042] In some embodiments, the instant anti-wrinkle cosmetic comprises the following components in mass percentages: 2%-20% of the instant anti-wrinkle composition from natural sources, 2%-5% of glycerol, 2%-5% of 1,3-propanediol, 0.01%-0.05% of xanthan gum, 0.01%-0.05% of hyaluronic acid, 0.2%-0.4% of acrylate / C10-30 alkyl acrylate crosslinked polymer, 0.1%-0.5% of p-hydroxyacetophenone, 0.02%-0.1% of L-arginine, 0.1-0.5% of hexylene glycol, and the balance of water.

[0043] In some embodiments, the instant anti-wrinkle cosmetic consists of the following components in mass percentages: 2%-20% of the instant anti-wrinkle composition from natural sources, 2%-5% of glycerol, 2%-5% of 1,3-propanediol, 0.01%-0.05% of xanthan gum, 0.01%-0.05% of hyaluronic acid, 0.2%-0.4% of acrylate / C10-30 alkyl acrylate crosslinked polymer, 0.1%-0.5% of p-hydroxyacetophenone, 0.02%-0.1% of L-arginine, 0.1-0.5% of hexylene glycol, and the balance of water.

[0044] Optionally, in the instant anti-wrinkle cosmetic, the mass percentage of the instant anti-wrinkle composition from natural sources is 2%, 5%, 8%, 10%, 12%, 15%, 18%, 20%. It can be understood that the mass percentage of the instant anti-wrinkle composition from natural sources in the instant anti-wrinkle cosmetic can also be other suitable choices within the range of 2% to 20%.

[0045] Optionally, in the instant anti-wrinkle cosmetic, the mass percentage of glycerol is 2%, 3%, 4%, 5%. It can be understood that the mass percentage of glycerol in the instant anti-wrinkle cosmetic can also be other suitable choices within the range of 2%-5%.

[0046] Optionally, in the instant anti-wrinkle cosmetic, the mass percentage of 1,3-propanediol is 2%, 3%, 4%, 5%. It can be understood that the mass percentage of 1,3-propanediol in the instant anti-wrinkle cosmetic can also be other suitable choices within the range of 2%-5%.

[0047] Optionally, in the instant anti-wrinkle cosmetic, the mass percentage of xanthan gum is 0.01%, 0.02%, 0.03%, 0.04%, 0.05%. It can be understood that the mass percentage of xanthan gum in the instant anti-wrinkle cosmetic can also be other suitable choices within the range of 0.01%-0.05%.

[0048] Optionally, in the instant anti-wrinkle cosmetic, the mass percentage of hyaluronic acid is 0.01%, 0.02%, 0.03%, 0.04%, 0.05%. It can be understood that the mass percentage of hyaluronic acid in the instant anti-wrinkle cosmetic can also be other suitable choices within the range of 0.01% - 0.05%.

[0049] Optionally, in the instant anti-wrinkle cosmetic, the mass percentage of acrylate / C10 - 30 alkyl acrylate cross-linked polymer is 0.2%, 0.25%, 0.3%, 0.35%, 0.4%. It can be understood that the mass percentage of acrylate / C10 - 30 alkyl acrylate cross-linked polymer in the instant anti-wrinkle cosmetic can also be other suitable choices within the range of 0.2% - 0.4%.

[0050] Optionally, in the instant anti-wrinkle cosmetic, the mass percentage of p-hydroxyacetophenone is 0.1%, 0.2%, 0.3%, 0.4%, 0.5%. It can be understood that the mass percentage of p-hydroxyacetophenone in the instant anti-wrinkle cosmetic can also be other suitable choices within the range of 0.1% - 0.5%.

[0051] Optionally, in the instant anti-wrinkle cosmetic, the mass percentage of L-arginine is 0.02%, 0.03%, 0.04%, 0.05%, 0.06%, 0.07%, 0.08%, 0.09%, 0.1%. It can be understood that the mass percentage of L-arginine in the instant anti-wrinkle cosmetic can also be other suitable choices within the range of 0.02% - 0.1%.

[0052] Optionally, in the instant anti-wrinkle cosmetic, the mass percentage of hexylene glycol is 0.1%, 0.2%, 0.3%, 0.4%, 0.5%. It can be understood that the mass percentage of hexylene glycol in the instant anti-wrinkle cosmetic can also be other suitable choices within the range of 0.1 - 0.5%.

[0053] In some embodiments, the instant anti-wrinkle cosmetic can be a cosmetic in the form of essence, essence milk, cream, eye cream, etc.

[0054] Another embodiment of the present invention provides a preparation method of the above-mentioned instant anti-wrinkle cosmetic. The preparation method includes the following steps: mixing each component evenly and performing homogenization treatment at 65°C - 75°C, then cooling and filtering to obtain the instant anti-wrinkle cosmetic. This preparation method is simple and easy to implement and is easy to promote.

[0055] Optionally, the temperature of the homogenization treatment can be 65°C, 66°C, 67°C, 68°C, 69°C, 70°C, 71°C, 72°C, 73°C, 74°C, 75°C. It can be understood that the temperature of the homogenization treatment can also be other suitable choices within the range of 65°C - 75°C.

[0056] In some embodiments, the homogenization time is 5 min to 15 min. For example, the homogenization time can be 5 min, 8 min, 10 min, 12 min, 15 min. It can be understood that other suitable selections can also be made within the range of 5 min to 15 min for the homogenization time.

[0057] In some embodiments, the preparation of the apple extract comprises the following steps: carefully select high-quality apples with appropriate maturity, plump fruits, and no pests or diseases. Wash the apples carefully to remove surface dirt and impurities. Peel, core, and cut the apples into pieces; subject them to vacuum freeze-drying technology at -40°C and 20 Pa until the water content ≤ 3%, and then crush to obtain freeze-dried powder with a particle size of 80 mesh. Mix the freeze-dried powder with pure water at a mass ratio of 1:15, and perform pulsed microwave treatment. The power of the pulsed microwave treatment is 600 W, the frequency is 2450 MHz, the pulse interval is 5 s, and the pulsed microwave treatment is repeated 3 times to obtain a pre-product. Place the pre-product in a supercritical fluid extraction kettle, use carbon dioxide as the supercritical fluid, at 35 MPa and 45°C, with an ethanol aqueous solution with a volume concentration of 10% as the entrainer, and perform dynamic extraction for 60 min to extract the fat-soluble components containing spherical collagen; adjust the system to 25 MPa and 55°C, switch the entrainer to a citric acid aqueous solution with a mass concentration of 5%, and perform dynamic extraction for 60 min to extract apple linear fibers; use molecular sieve chromatography to separate spherical collagen and linear apple fibers; then perform fractional purification by gradient ethanol precipitation. The volume concentration of ethanol in the gradient ethanol precipitation for fractional purification is 40%, 60%, and 80% in sequence; finally, obtain a white crystalline powder with a water content ≤ 3% through vacuum freeze-drying.

[0058] In some embodiments, the preparation of the peach resin extract comprises the following steps: dry the peach resin until the water content is below 10%, quickly freeze it with liquid nitrogen, and crush the material to a particle size of 120 mesh to obtain an active powder with a specific surface area ≥ 3.5 m 2 / g. Add pure water to the active powder according to a liquid-solid ratio of 1:10 - 1:15, and perform gradient temperature-variable extraction. The conditions for the gradient temperature-variable extraction are 2 h of extraction with pure water at 4°C, 1.5 h of extraction with a phosphate buffer solution (pH 6.8) at 25°C, and 1 h of extraction with an ethanol-aqueous solution containing 0.1% hyaluronidase by mass percentage at 40°C. The volume concentration of ethanol in the ethanol-aqueous solution is 30% to obtain an extraction product. The gradient temperature-variable extraction can gradually and gently dissolve the polysaccharide components in the peach resin extract, effectively avoiding the destruction of the polysaccharide structure during the extraction process.

[0059] The extracted product was centrifuged and fractionated through a 10 kDa ultrafiltration membrane. The first polysaccharide fraction with a molecular weight > 50 kDa and the second polysaccharide fraction with a molecular weight < 10 kDa were collected separately. Through the membrane separation system of 100 kDa and 30 kDa ultrafiltration membranes for fractional retention, the first polysaccharide was precipitated with an ethanol aqueous solution with a volume concentration of 60%, and the second polysaccharide was precipitated with an ethanol aqueous solution with a volume concentration of 80%. Finally, low-temperature vacuum concentration was carried out under the conditions of 45°C and 0.08 MPa; then spray drying was carried out to obtain the peach resin extract, with an inlet air temperature of 110°C and an outlet air temperature of 55°C for spray drying.

[0060] Optionally, when preparing the peach resin extract, ultrasonic-assisted treatment (frequency 40 kHz, power density 50 W / L) was carried out synchronously during the extraction process, and ultrasonic treatment was carried out for 20 minutes at each temperature change stage; the strong cavitation effect generated by ultrasonic waves can further break the microstructure of peach gum and promote the rapid release of polysaccharides.

[0061] Example 1

[0062] In this example, the composition consisted of the following components: 0.1 part of apple extract, 1 part of peach resin extract, and 0.01 part of hydroxyproline.

[0063] In this example, the essence consisted of the following components in mass percentages: 10% of the composition, 3% of glycerol, 3% of 1,3-propanediol, 0.01% of xanthan gum, 0.03% of hyaluronic acid, 0.25% of acrylate / C10-30 alkyl acrylate cross-linked polymer, 0.3% of p-hydroxyacetophenone, 0.06% of L-arginine, 0.3% of hexylene glycol, and the balance of water, and the sum of the mass percentages of each component was 100%.

[0064] Examples 2-3, Comparative Examples 1-3

[0065] Compared with Example 1, the differences in Examples 2-3 and Comparative Examples 1-3 were that the weight parts of each component in the composition were different, as specifically shown in Table 1.

[0066] Test Example

[0067] (1) Test subjects: 30 healthy people aged 30-60 years old, including 18 females and 12 males. The essence obtained from the examples and comparative examples was applied to the corners of the eyes on the left and right sides of the test subjects, and the change in the area of crow's feet of the test subjects over time was calculated. The average value of the reduction rate of the crow's feet area of 30 test subjects was taken as the result of the reduction rate of the crow's feet area corresponding to the examples and comparative examples. Among them, after 15 minutes of action, the reduction rates of the crow's feet area corresponding to the examples and comparative examples were as shown in Table 1. It can be seen from Table 1 that the essence in the examples had a significantly higher reduction rate of the crow's feet area than the comparative examples.

[0068] The crow's feet condition of one of the test subjects corresponding to the essence in Example 1 before and 15 minutes after use is as Figure 1 shown. Figure 1 In, (a) is the skin state diagram before using the essence, and (b) is the skin state diagram 15 minutes after using the essence. The area of the crow's feet circled in red in the figure. The area of the crow's feet in (a) is 4.83, and the proportion of the crow's feet area is 2.15%. The area of the crow's feet in (b) is 2.78, and the proportion of the crow's feet area is 1.23%. From Figure 1 it can be calculated that after using the essence in Example 1, the reduction rate of the crow's feet area is (4.83 - 2.78) / 4.83×100% = 42.4%. This indicates that the essence in Example 1 has a good immediate anti-wrinkle effect.

[0069] The change curve of the reduction rate of the crow's feet area corresponding to the essence in Example 1 with time is as Figure 2 shown. Figure 2 In, the abscissa represents time, the unit is min, and the ordinate is the reduction rate of the crow's feet area, the unit is %. From Figure 2 it can be seen that the essence in Example 1 has a good immediate anti-wrinkle effect and can obtain a good anti-wrinkle effect in a short time.

[0070] (2) The compositions in the examples and comparative examples are respectively formulated into aqueous solutions. Among them, the mass percentage of the corresponding composition in each aqueous solution is 10%. The inhibition rate of the aqueous solution on elastase activity is tested, and the results are shown in Table 1. It can be seen from Table 1 that the aqueous solution corresponding to the example has a significantly improved inhibition rate of elastase activity compared with the comparative example, indicating that the aqueous solution corresponding to the example has a better anti-wrinkle effect.

[0071] Table 1

[0072]

[0073]

[0074] (3) Test the irritation of the composition in Example 1. The chorioallantoic membrane (CAM) of the chicken embryo is a respiratory membrane that surrounds the chicken embryo. Since the surface of the chorioallantoic membrane of the chicken embryo is rich in blood vessels, it can be regarded as a complete organism. In this experiment, taking advantage of the characteristics of the complete, clear and transparent chorioallantoic membrane system of the mid-term hatched chicken embryo, the composition is directly contacted with the chorioallantoic membrane of the chicken embryo, and the toxic effect of the chorioallantoic membrane is observed 25 minutes after the action, so as to characterize the eye irritation of the composition. The results are as Figure 3 shown. Figure 3 In, (a) represents before the action, and (b) represents after the action. From Figure 3It can be seen that the composition in Example 1 is non-irritating to the eyes.

[0075] (4) The cytological model safety test was carried out on the composition in Example 1, and the test methods and results are shown in Table 2. It can be seen from Table 2 that the composition in Example 1 has good safety.

[0076] Table 2

[0077]

[0078] (5) Referring to the human skin patch test method in the "Technical Specifications for Cosmetics Safety" 2015 edition of China, the safety of the composition was further tested. The composition in Example 1 was formulated into an aqueous solution with a mass percentage of 30%. Test subjects: 30 healthy people aged 30 - 60, including 18 females and 12 males. The test times were 24 hours and 48 hours respectively, and the test results are shown in Table 3. It can be seen from Table 3 that no adverse reactions occurred in the test subjects after 24h and 48h. The results show that the composition still has good safety at a relatively high concentration, will not cause adverse skin reactions, and is safe and non-toxic.

[0079] Table 3

[0080]

[0081]

[0082] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, and improvements made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

[0083] For those of ordinary skill in the art, according to the idea of the present invention, there will be changes in the specific implementation manners and application scopes. In summary, the content of this specification should not be construed as a limitation to the present invention.

Claims

1. A natural instant wrinkle-removing composition, characterized in that: The invention comprises the following components in parts by weight: 0.1 to 6 parts of apple extract, 1 to 5 parts of peach resin extract and 0.01 to 0.5 parts of hydroxyproline.

2. Use of the natural instant wrinkle-removing composition according to claim 1 in the preparation of instant wrinkle-removing cosmetics.

3. The use according to claim 2, characterized in that The instant wrinkle removal includes instant reduction of the area of ​​wrinkles.

4. The use according to claim 3, characterized in that The wrinkles include at least one of nasolabial folds, forehead wrinkles, crow's feet and crow's feet at the corners of the eyes.

5. An instant anti-wrinkle cosmetic, characterized in that: The invention comprises the natural instant wrinkle-removing composition according to claim 1.

6. The instant anti-wrinkle cosmetic according to claim 5, characterized in that: The mass percentage of the natural-source instant wrinkle-removing composition in the instant wrinkle-removing cosmetic is 2%-20%.

7. The instant anti-wrinkle cosmetic according to claim 5 or 6, characterized in that: The following components are included in mass percentage: The instant wrinkle-removing composition of natural origin is 2%-20%, the preservative is 0.1%-2% and the balance is water.

8. The instant anti-wrinkle cosmetic according to claim 5 or 6, characterized in that: The invention comprises the following components in percentage by mass: 2%-20% of the instant anti-wrinkle composition of natural origin, 2%-5% of glycerol, 2%-5% of 1,3-propylene glycol, 0.01%-0.05% of xanthan gum, 0.01%-0.05% of hyaluronic acid, 0.2%-0.4% of acrylic acid (ester) / C10-30 alkyl acrylate cross-linked polymer, 0.1%-0.5% of p-hydroxyacetophenone, 0.02%-0.1% of L-arginine, 0.1-0.5% of hexylene glycol and the balance of water.

9. The method for preparing the instant anti-wrinkle cosmetic according to any one of claims 5 to 8, characterized in that: The method comprises the following steps: uniformly mixing the components and homogenizing them at 65-75° C., then cooling and filtering to obtain the instant anti-wrinkle cosmetics.

10. The method for preparing the instant anti-wrinkle cosmetic according to claim 9, characterized in that: The preparation of the apple extract comprises the following steps: peeling and core-removing apples and cutting them into pieces; treating the apples by vacuum freeze drying technology at -40°C and 20Pa until the water content is ≤3%, and then pulverizing to obtain a freeze-dried powder with a particle size of 80 meshes; mixing the freeze-dried powder with pure water at a mass ratio of 1:15, and treating the powder with a pulsed microwave at a power of 600W, a frequency of 2450MHz, and a pulse interval of 5s to obtain a pre-finished product; The pre-finished product is placed in a supercritical fluid extraction kettle, carbon dioxide is used as a supercritical fluid, and a 10% volume concentration ethanol aqueous solution is used as an entrainer under the conditions of 35MPa and 45°C, and dynamic extraction is performed for 60 minutes to extract the fat-soluble components containing globular collagen; The system was regulated to 25 MPa and 55°C, the entrainer was switched to a citric acid aqueous solution with a mass concentration of 5%, and the apple linear fibers were extracted by dynamic extraction for 60 minutes; the globular collagen and the linear apple fibers were separated by molecular sieve chromatography; the products were then graded and purified by gradient ethanol precipitation, and the volume concentrations of ethanol in the graded purification by gradient ethanol precipitation were 40%, 60%, and 80% respectively; finally, a white crystalline powder with a moisture content of ≤3% was obtained by vacuum freeze drying; The preparation of the peach resin extract comprises the following steps: drying the peach resin to a water content of less than 10%, quick freezing with liquid nitrogen, and crushing the material to a particle size of 120 meshes to obtain a specific surface area of ​​≥3.5m 2 / g of active powder; adding pure water to the active powder at a solid-liquid ratio of 1:10-1:15, and performing gradient temperature extraction, wherein the gradient temperature extraction conditions are 4°C pure water extraction for 2h, 25°C phosphate buffer extraction for 1.5h, and 40°C ethanol-water solution containing 0.1% hyaluronidase by mass for 1h, wherein the volume concentration of ethanol in the ethanol-water solution is 30%, to obtain an extraction product; centrifuging the extraction product, and classifying it through a 10kDa ultrafiltration membrane to collect the hyaluronidase with a molecular weight greater than 50kDa. The first-stage polysaccharide component and the second-stage polysaccharide component with a molecular weight less than 10kDa are graded and intercepted by a membrane separation system of 100kDa and 30kDa ultrafiltration membranes, the first-stage polysaccharide is precipitated by an ethanol aqueous solution with a volume concentration of 60%, and the second-stage polysaccharide is precipitated by an ethanol aqueous solution with a volume concentration of 80%, and finally low-temperature vacuum concentration is performed, and the conditions of low-temperature vacuum concentration are 45°C and 0.08MPa; then spray drying is performed to obtain peach resin extract, and the inlet air temperature of the spray drying is 110°C and the outlet air temperature is 55°C.

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