Method for relieving aluminum stress of water spinach

Abstract

The invention belongs to the technical field of plant stress resistance, and particularly relates to a method for relieving aluminum stress of water spinach. The invention discloses a method for relieving aluminum stress of water spinach. A relieving agent is sprayed on leaves of the water spinach; the mitigating agent is composed of a spermidine trihydrochloride solution and a strigolactone solution, the concentration of the spermidine trihydrochloride solution is 0.1-0.2 mmol / L, and the concentration of the strigolactone solution is 0.1-0.5 [mu] mol / L. The spermidine trihydrochloride solution and the strigolactone solution are combined for use, inhibition of aluminum stress on elongation and growth of water spinach root systems is relieved, meanwhile, aluminum absorption of the root systems can be reduced, the SOD activity of the root systems can be enhanced, then the influence of aluminum stress on growth of the water spinach can be effectively relieved, the aluminum resistance of the water spinach is improved, and the yield of the water spinach is increased. A support can be provided for developing a medicament for relieving the aluminum stress of the vegetables or improving the aluminum resistance of the vegetables.

Description

Technical Field

[0001] The present invention belongs to the technical field of plant stress resistance, and particularly relates to a method for alleviating aluminum stress in water spinach. Background Art

[0002] Water spinach is an alias of Ipomoea aquatica Forssk., and is an annual trailing herbaceous plant of the genus Ipomoea in the Convolvulaceae family.

[0003] Aluminum is the most abundant metallic element on the earth, mostly existing in the form of silicate in nature, and the solid-phase aluminum is not easily absorbed and utilized. Aluminum usually exists in the form of insoluble precipitates in the soil and is non-toxic to plants. However, when the soil is acidic or even the pH is lower than 5, the activity of aluminum increases, the bound aluminum is transformed into ionic state, and aluminum ions are released into the soil, causing toxicity to the plants in the soil. And as the pH decreases, the content of highly active aluminum rises rapidly, severely restricting the growth of plants. Soil acidification often occurs concomitantly with aluminum toxicity, and aluminum stress is the most important factor limiting plant production on acidic soils.

[0004] In addition, the excessive application of acidic nitrogen fertilizers in agricultural production, combined with the effects of acid rain deposition caused by environmental pollution and other aspects, has led to a sharp increase in the content of aluminum ions (Al 3+ ) in the soil. Aluminum is toxic to both the above-ground and underground parts of plants. However, relatively speaking, the toxic effect of aluminum on the above-ground part of plants generally lags behind that on the roots. The toxic effect of aluminum on plants is mainly manifested in the roots. The roots are the organs for plants to absorb water and nutrients, and aluminum toxicity blocks the cell division mechanism, resulting in the roots being unable to elongate through cell division, making the roots dry and brittle, the main roots thick and short, the root hairs underdeveloped, and the root tips swollen and brown in color. Aluminum toxicity has a certain inhibitory effect on the growth of the above-ground part. Therefore, there is an urgent need to develop agents for alleviating aluminum stress in vegetables or improving the aluminum tolerance of vegetables.

[0005] The information disclosed in this background art section is only intended to enhance the overall understanding of the present invention and should not be regarded as an admission or any form of implication that this information constitutes prior art already known to those of ordinary skill in the art. Summary of the Invention

[0006] The purpose of the present invention is to provide a method for alleviating aluminum stress in water spinach to solve the problems of aluminum stress on vegetables in the background art.

[0007] To achieve the above purpose, the present invention provides the following technical solutions:

[0008] A method for alleviating aluminum stress in water spinach, specifically: spraying a relieving agent on the leaves of water spinach; the relieving agent is composed of spermidine trihydrochloride solution and strigolactone solution, the concentration of the spermidine trihydrochloride solution is 0.1 - 0.2 mmol / L, and the concentration of the strigolactone solution is 0.1 - 0.5 μmol / L.

[0009] Specifically, the relieving agent is sprayed once every 7 days, and a total of 2 - 3 times are sprayed.

[0010] Specifically, the concentration of the spermidine trihydrochloride solution is 0.2 mmol / L, and the concentration of the strigolactone solution is 0.3 μmol / L.

[0011] Specifically, the preparation method of the spermidine trihydrochloride solution is: weigh spermidine trihydrochloride, dissolve it by magnetic stirring with ionic water, and then dilute it with deionized water to the required concentration.

[0012] Specifically, the preparation method of the strigolactone solution is: weigh strigolactone, vortex it with dimethyl sulfoxide until dissolved, and then dilute it with deionized water to the required concentration.

[0013] The present invention also provides a relieving agent, which is composed of spermidine trihydrochloride solution and strigolactone solution, the concentration of the spermidine trihydrochloride solution is 0.1 - 0.2 mmol / L, and the concentration of the strigolactone solution is 0.1 - 0.5 μmol / L.

[0014] Specifically, the concentration of the spermidine trihydrochloride solution is 0.2 mmol / L, and the concentration of the strigolactone solution is 0.3 μmol / L.

[0015] The present invention also provides the application of the relieving agent in alleviating aluminum stress in vegetables or improving the aluminum tolerance of vegetables.

[0016] Specifically, the vegetables include water spinach.

[0017] Compared with the prior art, the present invention has the following beneficial effects:

[0018] The present invention combines the spermidine trihydrochloride solution and the strigolactone solution, reduces the inhibition of aluminum stress on the root elongation growth of water spinach, and at the same time can also reduce the absorption of aluminum by the roots, enhance the SOD activity of the roots, and thus can effectively alleviate the influence of aluminum stress on the growth of water spinach and improve the aluminum tolerance of water spinach, providing support for the development of agents for alleviating aluminum stress in vegetables or improving the aluminum tolerance of vegetables. Specific embodiments

[0019] The technical solution of the present invention will be described clearly and completely below. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all of them. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative work belong to the scope of protection of the present invention.

[0020] 1. Materials and Methods

[0021] 1.1 Tested vegetables

[0022] Ipomoea aquatica "Thai willow leaf"

[0023] 1.2 Tested nutrient solution

[0024] Hogland 1-fold nutrient solution

[0025] 1.3 Cultivation of tested vegetables

[0026] Select tested vegetable seeds with uniform size and plump grains, disinfect them, germinate them until they show white tips, and then sow them in 72-well trays (the seedling substrate in the trays is composed of river sand, zeolite, and vermiculite mixed according to a mass ratio of 5:1:1). When they grow to the two true leaf stage, select healthy and consistent vegetable seedlings, wash the root substrate, and transfer them to a container containing clear water. After 1 day, transfer them to a hydroponic container wrapped with black plastic film containing Hogland 1-fold nutrient solution. After 3 days of adaptation, select healthy and consistent vegetable seedlings and transfer them to a hydroponic container (wrapped with black plastic film) containing stress treatment solution, and change the treatment solution every day. At the same time, set the treatment transferred to Hogland 1-fold nutrient solution as the blank control.

[0027] Stress treatment solution: Hogland 1-fold nutrient solution + AlCl3 solution, where the final concentration of AlCl3 is 50 μmol / L, and the pH of the stress treatment solution is adjusted to 4.5 ± 0.1 with 0.1 mol / L HCl solution (previous experiments found that under this pH condition, the aluminum stress on Ipomoea aquatica is relatively significant).

[0028] Cultivation conditions: light 16 h / d, day temperature 25 ± 1 °C, night temperature 25 ± 1 °C, relative humidity 70 ± 5%.

[0029] 1.4 Experimental treatments

[0030] For the Ipomoea aquatica seedlings in the hydroponic container with stress treatment solution, the following treatments are carried out respectively:

[0031] Treatment a: After transplanting to the hydroponic container with stress treatment solution for 2 days, spray distilled water on the leaf surface until it is completely wet, spray once every 7 days, for a total of 2 times.

[0032] Treatment b: After transplanting to the hydroponic container with stress treatment solution for 2 days, spray 0.2 mmol / L spermidine trihydrochloride solution on the leaf surface until it is completely wet, spray once every 7 days for a total of 2 times. The preparation method of the spermidine trihydrochloride solution is as follows: Weigh spermidine trihydrochloride, dissolve it with magnetic stirring in ionic water, and then dilute it with deionized water to the required concentration;

[0033] Treatment c: After transplanting to the hydroponic container with stress treatment solution for 2 days, spray 0.3 μmol / L strigolactone solution on the leaf surface until it is completely wet, spray once every 7 days for a total of 2 times. The preparation method of the strigolactone solution is as follows: Weigh strigolactone, vortex it in dimethyl sulfoxide until it dissolves, and then dilute it with deionized water to the required concentration.

[0034] Treatment d: After transplanting to the hydroponic container with stress treatment solution for 2 days, spray the compound agent (0.2 mmol / L spermidine trihydrochloride solution + 0.3 μmol / L strigolactone solution) on the leaf surface until it is completely wet, spray once every 7 days for a total of 2 times.

[0035] For the water spinach seedlings in the hydroponic container with 1-fold Hogland nutrient solution (i.e., the blank control), after transplanting to the hydroponic container with 1-fold Hogland nutrient solution for 2 days, spray distilled water on the leaf surface until it is completely wet, spray once every 7 days for a total of 2 times.

[0036] After the treatment is completed, transfer them to the original conditions for continued cultivation. Each treatment has 3 replicates, 1 replicate has 10 water spinach seedlings, and 1 water spinach seedling is in each hydroponic container.

[0037] 1.5 Index determination

[0038] Fourteen days after the treatment is completed, measure the following indexes

[0039] 1.5.1 Root relative elongation rate

[0040] Root length: Measure the main root length (from the root tip to the base of the stem) with a vernier caliper and calculate the relative elongation rate.

[0041] Relative elongation rate = (main root length of the treatment group / main root length of the blank control group) × 100%

[0042] 1.5.2 Root aluminum content

[0043] Take 2 cm of the root tip, dry it and digest it with nitric acid, and determine the aluminum content by AAS method.

[0044] 1.5.3 Root SOD activity

[0045] Use the micro method of the superoxide dismutase (SOD) activity detection kit provided by Sangon Biotech to determine the SOD activity of the root tip.

[0046] 1.6 Results and analysis

[0047] The results are shown in Table 1.

[0048] Table 1 Effects of Different Treatments on Alleviating Aluminum Stress in Water Spinach

[0049] Index Treatment a Treatment b Treatment c Treatment d Relative root elongation rate (%) 35.17 62.39 46.75 89.12 Root aluminum content (μg / g) 112.46 72.22 86.41 32.54 SOD activity (U / mg protein) 52.82 95.94 82.33 132.46

[0050] As can be seen from Table 1, in terms of the relative root elongation rate, under aluminum stress, the relative root elongation rates after treatment with spermidine trihydrochloride solution alone and strigolactone solution alone were 62.39% and 46.75% respectively, higher than 35.17% of the distilled water treatment, indicating that spraying spermidine trihydrochloride solution or strigolactone solution can alleviate the aluminum stress in water spinach. After treatment with the compound agent of spermidine trihydrochloride solution + strigolactone solution, the relative root elongation rate reached 89.12%, showing a significant effect compared with the treatment with spermidine trihydrochloride solution or strigolactone solution.

[0051] In terms of the aluminum content in roots, under aluminum stress, the aluminum contents in roots after treatment with spermidine trihydrochloride solution alone and strigolactone solution alone were 72.22 μg / g and 86.41 μg / g respectively, lower than 112.46 μg / g of the distilled water treatment, indicating that spraying spermidine trihydrochloride solution or strigolactone solution can reduce the aluminum absorption by roots, which helps to alleviate the aluminum stress in water spinach. After treatment with the compound agent of spermidine trihydrochloride solution + strigolactone solution, the aluminum content in roots was 32.54 μg / g, showing a significant effect compared with the treatment with spermidine trihydrochloride solution or strigolactone solution.

[0052] In terms of SOD activity, under aluminum stress, the SOD activities after treatment with spermidine trihydrochloride solution alone and strigolactone solution alone were 95.94 U / mg protein and 82.33 U / mg protein respectively, higher than 52.82 U / mg protein of the distilled water treatment, indicating that spraying spermidine trihydrochloride solution or strigolactone solution can enhance SOD activity to alleviate the aluminum stress in water spinach. After treatment with the compound agent of spermidine trihydrochloride solution + strigolactone solution, the SOD activity reached 132.46 U / mg protein, showing a significant effect compared with the treatment with spermidine trihydrochloride solution or strigolactone solution.

[0053] In summary, in the present invention, the combination of spermidine trihydrochloride solution and strigolactone solution is used to reduce the inhibition of aluminum stress on the root elongation growth of water spinach, and at the same time, it can also reduce the aluminum absorption by roots and enhance the SOD activity of roots, thereby effectively alleviating the influence of aluminum stress on the growth of water spinach and improving the aluminum tolerance of water spinach.

[0054] The foregoing description of the specific exemplary embodiments of the present invention is for purposes of illustration and exemplification. These descriptions are not intended to limit the invention to the precise forms disclosed, and it is apparent that many modifications and variations are possible in light of the above teachings. The purpose of selecting and describing the exemplary embodiments is to explain the specific principles of the invention and its practical applications, so that those skilled in the art can implement and utilize the various different exemplary embodiments of the invention, as well as various different selections and modifications. The scope of the present invention is intended to be defined by the claims and their equivalents.

Claims

1. A method for alleviating aluminum stress in water spinach, characterized in that: Specifically: spraying a mitigating agent on the leaves of water spinach; the mitigating agent consists of a spermidine trihydrochloride solution and a strigolactone solution, the concentration of the spermidine trihydrochloride solution is 0.1-0.2 mmol / L, and the concentration of the strigolactone solution is 0.1-0.5 μmol / L.

2. The method for alleviating aluminum stress of water spinach according to claim 1, characterized in that: The mitigation agent is sprayed once every 7 days, for a total of 2-3 times.

3. The method for alleviating aluminum stress of water spinach according to claim 1, characterized in that: The concentration of the spermidine trihydrochloride solution is 0.2 mmol / L, and the concentration of the strigolactone solution is 0.3 μmol / L.

4. The method for alleviating aluminum stress of water spinach according to claim 1, characterized in that: The preparation method of the spermidine trihydrochloride solution is: weigh spermidine trihydrochloride, add ionized water to dissolve it by magnetic stirring, and then add deionized water to dilute it to a required concentration.

5. The method for alleviating aluminum stress of water spinach according to claim 1, characterized in that: The preparation method of the strigolactone solution is as follows: weigh strigolactone, add dimethyl sulfoxide and vortex until dissolved, and then add deionized water to dilute to a desired concentration.

6. A mitigating agent, characterized in that The mitigating agent consists of a spermidine trihydrochloride solution and a strigolactone solution, wherein the concentration of the spermidine trihydrochloride solution is 0.1-0.2 mmol / L, and the concentration of the strigolactone solution is 0.1-0.5 μmol / L.

7. The mitigating agent according to claim 6, characterized in that The concentration of the spermidine trihydrochloride solution is 0.2 mmol / L, and the concentration of the strigolactone solution is 0.3 μmol / L.

8. Use of the mitigating agent according to claim 6 or claim 7 in alleviating aluminum stress of vegetables or improving aluminum tolerance of vegetables.

9. The use according to claim 8, characterized in that: The vegetables include water spinach.