Acne-removing and repairing composition specially researched by teenagers and application of acne-removing and repairing composition

Abstract

The invention discloses an acne removing and repairing composition specially researched by teenagers and application of the acne removing and repairing composition, and relates to the technical field of cosmetics. The invention provides an acne removing and repairing composition. The acne removing and repairing composition is prepared from the following components in parts by weight: 5 to 12 parts of guaiacol extract, 1 to 6 parts of solanaceous fruit extract, 0.001 to 0.01 part of escin, 0.01 to 0.1 part of diosgenin and 0.01 to 0.1 part of madecassoside. According to the acne treatment composition disclosed by the invention, the five components including the guaiacol extract, the solanaceous fruit extract, the aescin, the diosgenin and the madecassoside are mutually coordinated, so that three-dimensional coordination of anti-inflammation, repair and metabolic regulation is realized, and full-period management on acnes of teenagers and post-acne repair of the teenagers is formed.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to an anti-acne repair composition specifically developed for teenagers and its application. Background Art

[0002] Acne, as a chronic inflammatory skin disease with a high incidence among teenagers globally, its pathogenesis involves four core pathological links: (1) Androgen-mediated sebum gland hyperfunction: During puberty, the levels of androgens (testosterone, dihydrotestosterone) increase, stimulating the hyperplasia of sebaceous glands and excessive sebum secretion, resulting in shiny skin; (2) Abnormal follicular keratinization: The keratinocytes at the hair follicle duct proliferate excessively, blocking the pores and forming microcomedones (whiteheads or blackheads), which become the initial lesions of acne; (3) Imbalance of the microbial community: The anaerobic environment in the hair follicle promotes the formation of Propionibacterium acnes biofilm, and its metabolites trigger an inflammatory response; (4) Overactivation of the immune response: Neutrophil infiltration and cascading release of pro-inflammatory factors (IL-1α, IL-8, TNF-α) induce the overexpression of matrix metalloproteinases (MMPs), leading to the rupture of the hair follicle wall and deep dermal inflammation, and finally forming inflammatory papules, pustules or scar tissue.

[0003] The current clinical treatment faces the following technical pain points: ① Poor local drug tolerance: Although retinoid drugs (adapalene) can inhibit follicular keratinization and IL-6 secretion, their damage to the epidermal barrier function causes patients to terminate treatment due to erythema and desquamation; ② Restrictions on systemic treatment risks: Oral isotretinoin can inhibit the proliferation of sebaceous gland cells, but there are risks such as teratogenicity and liver damage, and strict contraception and liver function monitoring are required; ③ Misunderstandings in adjuvant treatment exacerbate the pathology: High-frequency soap-based facial cleansing destroys the skin's acidic protective film, promoting compensatory sebum secretion; Non-professional chemical peeling leads to thinning of the stratum corneum and increased sensitivity of TRPV1 receptors, inducing rosacea-like reactions; ④ Defects in patients' self-intervention: Under the misguidance of social media, squeezing skin lesions causes bacteria to spread, and the abuse of anti-acne products containing glucocorticoids induces telangiectasia and hormone-dependent dermatitis.

[0004] In view of this, the present application is proposed. Summary of the Invention

[0005] Based on this, the purpose of the present invention is to overcome the above-mentioned deficiencies of the prior art and provide an anti-acne repair composition specifically developed for teenagers and its application.

[0006] To achieve the above purpose, the technical solution adopted by the present invention is: An anti-acne repair composition, comprising the following components in parts by weight: 5-12 parts of guaiac extract, 1-6 parts of solanaceous fruit extract, 0.001-0.01 part of aescin, 0.01-0.1 part of diosgenin, 0.01-0.1 part of madecassoside.

[0007] In actual research, the inventors found that asiaticoside can stimulate the proliferation of fibroblasts and the synthesis of collagen, accelerate epidermal repair, reduce the formation of acne marks and scars; diosgenin can reduce deep tissue damage such as pits by inhibiting the fibrosis pathway, and at the same time induce apoptosis of abnormal cells to prevent repeated inflammation; aescin promotes local microcirculation and accelerates the excretion of metabolic wastes; the above three components work synergistically on superficial and deep repair, and can quickly promote the repair of skin cells. The guaiac extract and solanum fruit extract work synergistically with the above three components to further strengthen the skin barrier and form a three-dimensional repair network from the epidermis to the dermis. Moreover, in actual research, the inventors found that the five components of guaiac extract, solanum fruit extract, asiaticoside, diosgenin, and aescin work synergistically to balance the activity of sebaceous glands, reduce excessive oil secretion, prevent pore blockage; and inhibit lipid peroxidation, reduce abnormal follicular keratinization, and constrict capillaries, thereby regulating the skin's water-oil balance and metabolism and reducing the conditions for acne formation from the source.

[0008] In the acne repair composition of the present invention, appropriate weight parts of guaiac extract, solanum fruit extract, aescin, diosgenin, and asiaticoside are selected and added. The five components can work synergistically with each other, so as to effectively improve post-acne scars, reduce oil secretion, and repair the skin barrier, achieving remarkable effects on acne and its post-acne repair in teenagers. Through the synergistic effect of the five components of guaiac extract, solanum fruit extract, aescin, diosgenin, and asiaticoside, the present invention realizes the three-dimensional synergy of "anti-inflammatory-repair-metabolism regulation" and forms a full-cycle management for acne and its post-acne repair in teenagers.

[0009] Preferably, the component A includes the following components in parts by weight: 8-10 parts of guaiac extract, 2-4 parts of solanum fruit extract, 0.003-0.005 parts of aescin, 0.02-0.05 parts of diosgenin, and 0.05-0.08 parts of asiaticoside.

[0010] In actual research, the inventors found that the weight parts of the components in the composition provided by the present invention will affect the performance of the product. When the weight parts of the components are further selected within the above range, the obtained product has a more excellent three-dimensional synergistic effect of "anti-inflammatory-repair-metabolism regulation".

[0011] Preferably, based on the total weight of the acne repair composition, the weight ratio of the solanum fruit extract is 20-30%.

[0012] In actual research, the inventors found that when the weight ratio of the solanum fruit extract is within the above range based on the total weight of the acne repair composition, the obtained product has a more excellent three-dimensional synergistic effect of "anti-inflammatory-repair-metabolism regulation".

[0013] Preferably, the preparation method of the nightshade fruit extract comprises the following steps: taking nightshade fruits, drying, pulverizing, and sieving to obtain nightshade fruit coarse powder; then adding an extraction solvent for ultrasonic extraction, filtering by suction, concentrating and drying the extract to obtain the nightshade fruit extract; the extraction solvent is a deep eutectic solvent.

[0014] Preferably, the weight ratio of the extraction solvent to the nightshade fruit coarse powder is 10 - 20:1; and / or, the water content of the deep eutectic solvent is 20 - 40%; and / or, the temperature of the ultrasonic extraction is 30 - 60°C, the time of the ultrasonic extraction is 20 - 60 min, and the number of extraction times is 1 - 3 times.

[0015] Preferably, the deep eutectic solvent is diluted with deionized water to obtain deep eutectic solvents with different water contents.

[0016] Preferably, the hydrogen bond acceptor of the deep eutectic solvent is choline chloride, and the hydrogen bond donor of the deep eutectic solvent is at least one of lactic acid, ethylene glycol, urea, citric acid, and glucose.

[0017] Preferably, the molar ratio of the hydrogen bond acceptor to the hydrogen bond donor is 1:2 - 4.

[0018] The inventors found in actual research that when preparing nightshade fruit extracts by different extraction methods, it will affect the contents of flavonoids and polyphenols in the nightshade fruit extracts, such as anthocyanins and chlorogenic acids, etc. Furthermore, when the nightshade fruit extracts prepared by different extraction methods are added to the acne repair composition, the efficacy of the obtained acne repair composition will be different. When the preparation method is selected as above, the comprehensive effect of the obtained composition is better.

[0019] In addition, the present invention provides the application of the acne repair composition in the preparation of skin care products.

[0020] Furthermore, the present invention provides a skin care product, and the skin care product comprises the acne repair composition. Preferably, the skin care product comprises any one of lotion, emulsion, paste, cream, facial mask, essence, and spray.

[0021] Preferably, the skin care product comprises the following components in mass percentage: 1 - 3% of the acne repair composition, 2 - 40% of the cosmetic matrix, and the balance is water.

[0022] Preferably, the cosmetic matrix comprises at least one of a thickener, a humectant, an emulsifier, a preservative, a fragrance, and a pH regulator.

[0023] Preferably, the skin care product comprises the following components in mass percentages: 1-3% of acne-removing and repairing composition, 0.05-0.5% of thickener, 3-20% of moisturizer, 0.5-10% of emulsifier, 0.2-5% of preservative, 0.01-0.2% of essence, 0.05-0.1% of pH regulator, and the balance is water.

[0024] Exemplarily, the thickener includes at least one of xanthan gum, carbopol 940, AVC, and high molecular cellulose; the moisturizer includes at least one of glycerol, butanediol, and low molecular weight sodium hyaluronate; the emulsifier includes at least one of coco-caprylate / caprate, polydimethylsiloxane, hydrogenated palm kernel oil, cetearyl alcohol, polyglyceryl-6 distearate, and polymethylsilsesquioxane; the preservative includes at least one of 1,3-propanediol, 1,2-hexanediol, and p-hydroxyacetophenone; the pH regulator includes at least one of arginine and EDTA-2Na.

[0025] Compared with the prior art, the beneficial effects of the present invention are as follows: In the acne-removing and repairing composition of the present invention, appropriate weight portions of guaiac extract, solanaceous fruit extract, aescin, diosgenin, and asiaticoside are selected and added. The five components can cooperate with each other, so as to effectively improve post-acne scars, reduce sebum secretion, and repair the skin barrier, achieving significant effects on acne and post-acne repair of teenagers. The present invention realizes the three-dimensional cooperation of "anti-inflammatory-repair-metabolic regulation" through the mutual cooperation of the five components of guaiac extract, solanaceous fruit extract, aescin, diosgenin, and asiaticoside, and forms a full-cycle management for acne and post-acne repair of teenagers. Description of the Drawings

[0026] Figure 1 It is the 6h cell migration map of the blank control group, comparative example 1, and example 1 in performance test-1;

[0027] Figure 2 It is the 24h cell migration map of the blank control group, comparative example 1, and example 1 in performance test-1;

[0028] Figure 3 It is the acne improvement map of application example 1 at 0, 3, and 14 days in performance test-3;

[0029] Figure 4 It is the acne improvement map of comparative application example 1 at 0, 3, and 14 days in performance test-3. Detailed Embodiments

[0030] To better illustrate the purpose, technical solution and advantages of the present invention, the present invention will be further described below in conjunction with the accompanying drawings and specific embodiments. The purpose is to understand the content of the present invention in detail, rather than to limit the present invention. All other embodiments obtained by those of ordinary skill in the art without creative efforts fall within the protection scope of the present invention. The experimental reagents and instruments designed in the examples and comparative examples of the present invention are all common ordinary reagents and instruments, unless otherwise specified, and can all be obtained through commercial channels. In the examples and comparative examples, the experimental methods used are all conventional methods unless otherwise specified; and without special instructions, the raw materials used in parallel experiments are from the same batch of raw materials.

[0031] The raw materials of the present invention are described as follows, but are not limited to the following raw materials:

[0032] Guaiac extract: purchased from Shanghai Aladdin Biochemical Technology Co., Ltd.;

[0033] Aescin: purchased from Yuanye Bio-Technology Co., Ltd.;

[0034] Diosgenin: purchased from ANPEL Laboratory Technologies Inc.;

[0035] Madecassoside: purchased from J&K Scientific Ltd.;

[0036] Licorice extract: purchased from Merck Chemical Technology Co., Ltd.;

[0037] Astragaloside: purchased from Jingcui Tiancheng Pharmaceutical Technology Co., Ltd.;

[0038] Asiaticoside: purchased from J&K Scientific Ltd.;

[0039] Smilagenin: purchased from Dest Biotechnology Co., Ltd.;

[0040] Tomato fruit extract - 1: self - made, and the preparation method is as follows: Take the cleaned tomato fruits, dry and crush them in an oven at 60 °C, and pass through a 40 - mesh sieve to obtain tomato fruit coarse powder. Then add the extraction solvent and carry out ultrasonic extraction, filter by suction, concentrate and dry the extractive solution to obtain tomato fruit extract; the extraction solvent is eutectic solvent - 1, and the water content of the eutectic solvent is 30%; the weight ratio of the eutectic solvent to the tomato fruit coarse powder is 15:1; the ultrasonic extraction is carried out for 30 min, the ultrasonic extraction temperature is 40 °C, and the extraction times are 2 times.

[0041] Tomato fruit extract - 2: self - made, and the only difference from the preparation method of tomato fruit extract - 1 is that: the extraction solvent is eutectic solvent - 2.

[0042] Tomato fruit extract - 3: self - made, and the only difference from the preparation method of tomato fruit extract - 1 is that: the extraction solvent is eutectic solvent - 3.

[0043] Solanaceous fruit extract - 4: Self - made. The only difference from the preparation method of Solanaceous fruit extract - 1 is that the extraction solvent is eutectic solvent - 4.

[0044] Solanaceous fruit extract - 5: Self - made. The only difference from the preparation method of Solanaceous fruit extract - 1 is that the extraction solvent is eutectic solvent - 5.

[0045] Solanaceous fruit extract - 6: Self - made. The only difference from the preparation method of Solanaceous fruit extract - 1 is that the extraction solvent is eutectic solvent - 6.

[0046] Solanaceous fruit extract - 7: Self - made. The only difference from the preparation method of Solanaceous fruit extract - 1 is that the extraction solvent is eutectic solvent - 7.

[0047] Solanaceous fruit extract - 8: Self - made. The only difference from the preparation method of Solanaceous fruit extract - 1 is that the extraction solvent is eutectic solvent - 8.

[0048] Solanaceous fruit extract - 9: Self - made. The only difference from the preparation method of Solanaceous fruit extract - 1 is that the extraction solvent is eutectic solvent - 9.

[0049] Solanaceous fruit extract - 10: Self - made. The only difference from the preparation method of Solanaceous fruit extract - 1 is that the extraction solvent is an aqueous ethanol solution with a volume fraction of 60%.

[0050] The components and their mass ratio of the eutectic solvent are selected as shown in Table 1. The preparation method of the eutectic solvent is as follows: Place the hydrogen - bond acceptor and hydrogen - bond donor in an 80 °C constant - temperature water bath and mix evenly. After cooling to room temperature, dilute with deionized water to different water contents to obtain the eutectic solvent.

[0051] Table 1

[0052]

[0053] Examples 1 - 14 and Comparative Examples 1 - 11

[0054] The examples and comparative examples of the present invention provide an acne - removing and repairing composition. The components and their parts by weight of the composition are selected as shown in Tables 2 - 3. The preparation method of the composition includes the following steps: Mix each component evenly to obtain the acne - removing and repairing composition.

[0055] Table 2

[0056]

[0057]

[0058] Table 3

[0059]

[0060]

[0061] Application Examples and Comparative Application Examples

[0062] Application Examples 1 - 15, Comparative Application Examples 1 - 11, and Blank Application Example of the present invention provide emulsions containing an anti - acne repair composition. The components (mass percentage) of the emulsions are shown in Table 4; among them, the anti - acne repair compositions in Application Examples 1 - 14 are respectively the anti - acne repair compositions prepared in Examples 1 - 14, and the anti - acne repair compositions in Comparative Application Examples 1 - 11 are respectively the anti - acne repair compositions prepared in Comparative Examples 1 - 11; for example, the anti - acne repair composition used in Application Example 1 is the anti - acne repair composition prepared in Example 1, the anti - acne repair composition used in Application Example 2 is the anti - acne repair composition prepared in Example 2, the anti - acne repair composition used in Application Example 15 is the anti - acne repair composition prepared in Example 1, the anti - acne repair composition used in Comparative Application Example 1 is the anti - acne repair composition prepared in Comparative Example 1, and so on.

[0063] Table 4

[0064]

[0065]

[0066] The preparation method of the emulsion provided in Application Example 1 is as follows:

[0067] (1) Mix the Phase A with water and stir. After heating to 80 °C, homogenize at a speed of 1200 rpm for 5 min. After the homogenization is completed, keep it warm for standby to obtain the pre - prepared Phase A;

[0068] (2) Mix the Phase B, heat to 80 °C, then homogenize at a speed of 1200 rpm for 5 min. After the homogenization is completed, keep it warm for standby to obtain the pre - prepared Phase B;

[0069] (3) Mix the Phase C, heat to 60 °C to melt, to obtain the pre - prepared Phase C;

[0070] (4) Heat the pre - prepared Phase A to 80 °C, add the pre - prepared Phase B at a speed of 300 rpm, stir and mix. Then cool down to 60 °C, add the pre - prepared Phase C at a speed of 300 rpm and stir and mix. Subsequently, cool down to below 45 °C, add the anti - acne repair composition and essence and continue to stir for 5 min. Finally, add arginine to adjust the pH to 5.0 - 6.5, then stop stirring, discharge, to obtain the emulsion.

[0071] The preparation methods of the emulsions provided in the remaining Application Examples, Comparative Application Examples, and Blank Application Example are the same as that of Application Example 1. If there are no relevant components, just do not add them.

[0072] Performance Test - 1 Skin Cell Repair Test

[0073] Explore the efficacy of the compositions prepared in Examples 1 - 14 and Comparative Examples 1 - 11 as follows:

[0074] On monolayer adherent cells cultured in vitro in petri dishes or plates, use a pipette tip or other hard object to draw a line in the central area where the cells grow, remove the cells in the central part, and then continue to culture the cells until the time set in the experiment. According to the ability of the cells at the scratch edge to gradually enter the blank area to make the "scratch" heal, judge the cell growth and migration ability. It can simulate the wound healing process and is used to evaluate the migration ability of cells at the wound site.

[0075] Experimental materials: Cell line: HaCaT (human immortalized keratinocytes), P17, purchased from Beina Biological. Fetal bovine serum: Gibco, DMEM basal medium, phosphate buffer solution (PBS), double antibody (penicillin (100 U / mL), streptomycin (100 μg / mL)), 0.25% trypsin, cell culture flasks and multi-well cell culture plates, 0.22 μm microporous filter membrane, wound healing 2-well insert (ibidi, REF: 80209).

[0076] Experimental procedure: Take out a 12-well plate, use sterile forceps to fix a 2-well wound healing insert in the middle of the corresponding well plate, and gently press the healing insert to fix it in the well plate (wound healing 2-well insert ibidi, purchased from Shanghai Huayasi Chuang Biotechnology Co., Ltd., product number 80209). Inoculate the cells at a density of 4.0×10 5 cells / 70 μL / well into the 2-well wound healing insert, with 3 replicates for each group, and return to the incubator for culture until the cells are confluent. Take out the culture plate, remove the 2-well wound insert to create a "wound". Wash with PBS to remove the detached cells. Replace the blank group with a culture medium containing 0.1% fetal bovine serum, and replace the test substance group with a culture medium containing 0.1% fetal bovine serum and the test substance. Image acquisition, collect images at fixed positions, and record the cell migration conditions at 0 h, 6 h, and 24 h respectively.

[0077] Data analysis: Observe the scratch areas of the cell control wells and each sample well at 0 h - 6 h - 24 h. Use imageJ software for image analysis, measure the scratch area using Image J software, and calculate the migration status of the cells in the scratch area; take the scratch area at 0 h as 100%, and calculate the relative size of the scratch area at each time point for each group;

[0078] Cell migration rate = (scratch area at 0 h - scratch area after culture) / scratch area at 0 h × 100%.

[0079] The results are shown in Table 5.

[0080] Table 5

[0081] Group Cell migration rate at 6h (%) Cell migration rate at 24h (%) Blank control group 15.13 40.30 Example 1 34.27 85.22 Example 2 28.79 78.58 Example 3 28.11 77.11 Example 4 27.68 76.65 Example 5 27.04 75.37 Example 6 33.60 83.90 Example 7 26.01 73.11 Example 8 26.05 73.13 Example 9 32.94 83.08 Example 10 25.26 70.86 Example 11 34.25 85.20 Example 12 30.17 77.58 Example 13 30.21 77.30 Example 14 32.89 81.11 Comparative example 1 17.84 49.27 Comparative example 2 17.97 49.25 Comparative example 3 18.67 51.81 Comparative example 4 18.56 51.79 Comparative example 5 18.88 51.76 Comparative example 6 18.80 51.85 Comparative example 7 19.68 52.76 Comparative example 8 21.80 53.23 Comparative example 9 22.70 54.35 Comparative example 10 22.68 54.31 Comparative example 11 22.62 54.28

[0082] As can be seen from the above table, the composition prepared in the embodiment of the present invention has a good effect of promoting the repair of skin cells, with a fast repair speed and a good repair effect. The cell migration rate reaches more than 25% at 6h and more than 70% at 24h. Figure 1 Cell migration diagrams at 6h for the blank control group, Comparative Example 1 and Example 1;

[0083] Figure 2 Cell migration diagrams at 24h for the blank control group, Comparative Example 1 and Example 1 in Performance Test - 1. As can be seen from the figures, the composition prepared in the present invention has a good effect of promoting the repair of skin cells, with a fast repair speed and a good repair effect.

[0084] From the comparison of Examples 1 - 10, it can be seen that when tomato fruit extracts prepared by different extraction methods are added to the acne - removing and repair composition, there will be differences in the efficacy of the obtained acne - removing and repair composition. In particular, the choice of extraction solvent in the extraction process has a greater impact. When the deep eutectic solvent with choline chloride as the hydrogen bond acceptor and lactic acid as the hydrogen bond donor is selected, the effect of promoting the repair of skin cells is better.

[0085] From the comparison of Example 1, Examples 11 - 14, it can be seen that the component weight parts in the composition will affect the performance of the product. When the acne - removing and repair composition includes the following components in parts by weight: guaiac extract 8 - 10 parts, tomato fruit extract 2 - 4 parts, aescin 0.003 - 0.005 parts, diosgenin 0.02 - 0.05 parts, asiaticoside 0.05 - 0.08 parts, the effect of promoting the repair of skin cells is better.

[0086] From the comparison of Example 1, Comparative Examples 1 - 11, it can be seen that when one or two of the components are not added, or when the mass part of one component is added to other components when it is not added, the effects of the present invention cannot be achieved.

[0087] Compared with Example 1, in Comparative Examples 8 - 11, when other components are used to replace the components in the present invention, although the cell migration rate at 6h can reach more than 20%, the cell migration rate at 24h has a large gap with Example 1, and the effect of promoting the repair of skin cells is also not good. The five components in the present invention cooperate with each other and are indispensable.

[0088] Performance Test - 2 Anti - inflammatory Effect Test

[0089] To explore the efficacy of the compositions prepared in Examples 1 - 14 and Comparative Examples 1 - 11, specifically as follows:

[0090] Experimental materials: The cell line used was mouse macrophage RAW264.7, purchased from the Cell Resource Center of the Institute of Basic Medicine, Chinese Academy of Medical Sciences, fetal bovine serum (FBS, Gibco), high-glucose DMEM medium (DMEM, Gibco), dimethyl sulfoxide (DMSO, Sigma), phosphate buffer (PBS, Gibco), thiazolyl blue (MTT, Sigma), mouse tumor necrosis factor-α enzyme-linked immunosorbent assay kit (Mouse TNF-α ELISA kit, Boster), and mouse interleukin-1β enzyme-linked immunosorbent assay kit (Mouse IL-1β ELISA kit, Boster).

[0091] The experimental groups were set as shown in Table 6. The specific operations were as follows:

[0092] (1) Seeding: The cells were seeded into 24-well plates and incubated in a 37°C, 5% CO2 incubator for 24 h;

[0093] (2) Preparation of solutions: Prepare the test substances and positive controls according to Table 6;

[0094] (3) Administration of samples: According to the experimental grouping and concentration settings in Table 6, after the cells in the 24-well plates had grown for 24 h, group administration of samples was carried out. Each treatment group had 3 replicate wells. The blank control and negative control groups were both added with cell culture medium, and the sample groups were added with cell culture medium containing the corresponding concentration of samples. They were then continued to be cultured in a 37°C, 5% CO2 incubator for 24 h;

[0095] (4) LPS induction: After culturing for 24 h, the culture medium in the plates was aspirated, and all were gently washed once with PBS. The blank control group was added with cell culture medium, and the negative control group, sample groups, and positive control group were added with cell culture medium containing LPS. After continuing to culture in a 37°C, 5% CO2 incubator for 24 h, the supernatant was collected;

[0096] (5) Detection of inflammatory factor content: Take the cell supernatant of each well and detect the content of cell inflammatory factors according to the operation instructions of the ELISA kit; Inflammatory factor inhibition rate (%) = (Average concentration of the negative control group - Average concentration of the sample group) / Average concentration of the negative control group × 100%.

[0097] (6) Data processing: All data obtained in the experiment were processed and graphed using Excel software, and the results are shown in Table 7.

[0098] Table 6

[0099]

[0100] Table 7

[0101]

[0102]

[0103] As can be seen from the above table, the composition prepared in the embodiment of the present invention has a good anti-inflammatory effect, and the inhibition rate of the inflammatory factor IL-1β reaches more than 50%.

[0104] From the comparison of Examples 1-10, it can be seen that when the nightshade fruit extract prepared by different extraction methods is added to the acne repair composition, the efficacy of the obtained acne repair composition will be different. In particular, the selection of the extraction solvent in the extraction process has a greater impact. When the deep eutectic solvent with choline chloride as the hydrogen bond acceptor and lactic acid as the hydrogen bond donor is selected, the anti-inflammatory effect is better.

[0105] From the comparison of Example 1 and Examples 11-14, it can be seen that the component weight parts in the composition will affect the performance of the product. When the acne repair composition includes the following components in parts by weight: guaiac extract 8-10 parts, nightshade fruit extract 2-4 parts, aescin 0.003-0.005 parts, diosgenin 0.02-0.05 parts, asiaticoside 0.05-0.08 parts, the anti-inflammatory effect is better.

[0106] From the comparison of Example 1 and Comparative Examples 1-11, it can be seen that when one or two of the components are not added, or when the mass part of one component is added to other components when one component is not added, the effects of the present invention cannot be achieved; and when other components are used to replace the components in the present invention, the anti-inflammatory effect is also not good. The five components in the present invention cooperate with each other and are indispensable.

[0107] Performance Test - 3 Human Efficacy Test

[0108] Explore the efficacy of the lotions containing the acne repair composition provided by Application Examples 1-15, Comparative Application Examples 1-11 and Blank Application Example, as follows:

[0109] This effect example tested the effects of the lotions prepared by the application examples, comparative application examples and blank application example on the skin TEWL, acne count, oil content and brightness improvement of the human body.

[0110] The specific test method is as follows:

[0111] Select sensitive skin volunteers aged 18 - 50 years old (lactic acid stinging score > 3) according to the "Technical Specifications for Cosmetics Safety" (2015). Randomly divide them into 27 groups with 5 people in each group. Volunteers should have at least 2 - 3 obvious pimples. Under normal circumstances, volunteers continuously use the product on the whole face for 14 days. After cleansing and moisturizing the face in the morning and evening, volunteers take an appropriate amount of lotion and apply it on the face, and massage until absorbed. The pimple area can be overlaid. Use it once in the morning and once in the evening on the whole face. Make a follow-up visit on the 3rd day and the 14th day. On the day when the volunteer arrives, after washing the test area with clean water, do not apply any product, and sit quietly in an air-conditioned room with a temperature of 21 ± 1°C and a humidity of 50 ± 10% for 20 minutes.

[0112] Use instruments to quantitatively analyze and photograph the improvement of facial acne indicators, including acne count (evaluated by VISIA and doctors respectively), Tewameter TM Hex test for trans-epidermal water loss TEWL, Sebumeter SM 815 test for oil content, and Skin-Colorimeter CL400 test for brightness. The improvement rate before and after use = |mean value before use - mean value after use| / mean value before use × 100%; The calculation results are shown in Table 8.

[0113] Table 8

[0114]

[0115]

[0116]

[0117] As can be seen from the above table, the lotion prepared in the application example of the present invention has good three-dimensional synergy of "anti-inflammatory - repair - metabolic regulation". Figure 3 It is the acne improvement diagrams at 0, 3, and 14 days of Application Example 1; Figure 4 It is the acne improvement diagrams at 0, 3, and 14 days of Comparative Application Example 1. By comparison, it can be seen that the lotion prepared by the present invention has a good effect on improving acne.

[0118] By comparing Application Examples 1 - 10, it can be seen that when preparing the nightshade fruit extract by different extraction methods and adding it to the acne-removing and repair composition, the efficacy of the obtained acne-removing and repair composition will be different. In particular, the choice of extraction solvent in the extraction process has a greater impact. When choosing a deep eutectic solvent with choline chloride as the hydrogen bond acceptor and lactic acid as the hydrogen bond donor, the three-dimensional synergy effect of "anti-inflammatory - repair - metabolic regulation" is better.

[0119] From the comparison between Application Example 1 and Application Examples 11 - 14, it can be seen that the parts by weight of the components in the composition will affect the performance of the product. When the acne treatment and repair composition includes the following components in parts by weight: guaiac extract 8 - 10 parts, solanaceous fruit extract 2 - 4 parts, aescin 0.003 - 0.005 parts, diosgenin 0.02 - 0.05 parts, asiaticoside 0.05 - 0.08 parts, the "three - dimensional synergy of anti - inflammation - repair - metabolic regulation" is better.

[0120] From the comparison between Application Example 1 and Comparative Application Examples 1 - 11, it can be seen that when one or two of the components are not added, or when the mass parts of one component are added to other components when it is not added, the effects of the present invention cannot be achieved; and when other components are used to replace the components in the present invention, the "three - dimensional synergy of anti - inflammation - repair - metabolic regulation" is not good either. The five components in the present invention synergize with each other and are all indispensable.

[0121] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit the protection scope of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that the technical solutions of the present invention can be modified or equivalently replaced without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. An acne-removing and repairing composition, characterized in that: The invention comprises the following ingredients in parts by weight: 5-12 parts of Guaiac wood extract, 1-6 parts of Solanum lycopersicum extract, 0.001-0.01 parts of aescin, 0.01-0.1 parts of diosgenin and 0.01-0.1 parts of madecassoside.

2. The acne-removing and repairing composition according to claim 1, characterized in that: The invention comprises the following ingredients in parts by weight: 8-10 parts of Guaiac wood extract, 2-4 parts of Solanum lycopersicum extract, 0.003-0.005 parts of aescin, 0.02-0.05 parts of diosgenin and 0.05-0.08 parts of madecassoside.

3. The acne-removing and repairing composition according to claim 1, characterized in that: Based on the total weight of the acne-repairing composition, the weight of the Solanum fruit extract accounts for 20-30%.

4. The acne-removing and repairing composition according to claim 1, characterized in that: The preparation method of the eggplant fruit extract comprises the following steps: taking the eggplant fruit, drying, crushing and sieving to obtain eggplant fruit coarse powder; then adding an extraction solvent for ultrasonic extraction, filtering, concentrating and drying the extract to obtain the eggplant fruit extract; the extraction solvent is a low eutectic solvent.

5. The acne-removing and repairing composition according to claim 4, characterized in that: The weight ratio of the extraction solvent to the eggplant coarse powder is 10-20:1; and / or the water content of the low eutectic solvent is 20-40%; and / or the temperature of the ultrasonic extraction is 30-60°C, the time of the ultrasonic extraction is 20-60min, and the number of extractions is 1-3 times.

6. The acne-removing and repairing composition according to claim 4, characterized in that: The hydrogen bond acceptor of the low eutectic solvent is choline chloride, and the hydrogen bond donor of the low eutectic solvent is at least one of lactic acid, ethylene glycol, urea, citric acid, and glucose.

7. The acne-repairing composition according to claim 6, characterized in that: The molar ratio of the hydrogen bond acceptor to the hydrogen bond donor is 1:2-4.

8. Use of the acne-repairing composition according to any one of claims 1 to 7 in the preparation of skin products.

9. A skin care product, characterized in that: The skin product comprises the acne-repairing composition according to any one of claims 1 to 7.

10. The skin care product according to claim 9, wherein: The skin product comprises the following components in percentage by mass: 1-3% of acne-repairing composition, 2-40% of cosmetic base, and the balance of water.

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