Method for constructing trichosanthes kirilowii maxim medicinal material fingerprint spectrum and determining content of trichosanthes kirilowii maxim index components

By constructing the fingerprint map and quantitative analysis method of the Trichosanthes medicinal materials, and using LC-MS technology, the problem of difficulty in consistent evaluation of the quality of Trichosanthes medicinal materials in the existing technology was solved, and a comprehensive and controllable evaluation and analysis of the quality of Trichosanthes medicinal materials was achieved.

CN120214169APending Publication Date: 2025-06-27MACAU UNIV OF SCI & TECH
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Patent Information

Application Number
CN202510521466.4
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2023-03-28
Publication Date
2025-06-27

AI Technical Summary

Technical Problem

The prior art lacks effective methods to evaluate the consistency of the quality of Trichosanthes medicinal materials, which makes it difficult to control the overall quality of Trichosanthes medicinal materials.

Method used

By constructing the fingerprint map of the Trichosanthes medicinal materials, using liquid chromatography-mass spectrometry (LC-MS) technology, the index components in Trichosanthes kirily are isolated and identified, and quantitative analysis methods are established to achieve a comprehensive evaluation of the quality of Trichosanthes medicinal materials.

Benefits of technology

This method realizes the effective separation and quantitative analysis of the index components of Trichosanthes medicinal materials, establishes stable evaluation standards, and ensures the controllability of the quality of the medicinal materials and the effectiveness of clinical use.

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Abstract

The invention relates to a method for constructing a trichosanthes kirilowii maxim medicinal material fingerprint spectrum and determining the content of trichosanthes kirilowii maxim index components. The method comprises the following steps: taking a trichosanthes kirilowii maxim medicinal material to prepare a test solution; taking rutin, chrysoeriol and cucurbitacine D to prepare a reference substance solution; respectively injecting the test solution and the reference solution into a liquid chromatograph-mass spectrometer, obtaining a fingerprint spectrum of the trichosanthes kirilowii maxim medicinal material according to the liquid chromatograph-mass spectrometer, establishing a UHPLC-QQQ-MS quantitative detection method, and determining the content of the index components in the trichosanthes kirilowii maxim medicinal material in an MRM mode. According to the method for constructing the snakegourd fruit medicinal material fingerprint spectrum and measuring the content, provided by the invention, the evaluation standard of snakegourd fruits is comprehensively improved, and the effectiveness of the snakegourd fruit medicinal material in clinical use is ensured.
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