Intelligence-promoting nutritional formula growth milk powder for children as well as preparation method and application thereof

Abstract

The invention provides intelligence-promoting nutritional formula growth milk powder for children as well as a preparation method and application thereof, and belongs to the technical field of preparation of dairy products. The nutritional formula milk powder for children, provided by the invention, contains various intelligence-promoting components, and docosahexaenoic acid, arachidonic acid, phosphatidylserine and xanthophyll have a synergistic effect, so that brain and vision development of children is comprehensively promoted; a compound protein system is included, so that the protein biological value reaches 90%, the protein absorption rate is increased, and the allergy risk is reduced; the vitamin component and the xanthophyll can generate a coordination effect, so that the free radical scavenging capability is improved, and the anti-fatigue effect is enhanced; meanwhile, the probiotics and the prebiotics can also construct a probiotics-prebiotics symbiotic system, so that the intestinal microecology is improved. According to the invention, the defects of the existing milk powder for children in the aspects of intelligence development, absorption, immunity and palatability are systematically solved through a composite intelligence-promoting component, a protein and probiotic synergistic system and antioxidant chain reaction optimization, and a multi-dimensional competitive advantage is formed.

Description

Technical Field

[0001] The present invention belongs to the technical field of dairy product preparation, and particularly relates to a children's intelligent nutrition formula growth milk powder and its preparation method and application. Background Art

[0002] Childhood is an important period for the growth and development of the human body. At this time, it is in a stage of rapid growth and development. Therefore, at this stage, children have extremely high requirements for the comprehensiveness, balance and functionality of nutrition. Good nutrition and scientific feeding at this stage are the most important guarantees for the short-term and long-term health of children. Among them, nutrition is an important environmental factor. Nutrition and development in childhood have a long-term and crucial impact on physical growth, intellectual development, immune function and other aspects of health in the short term and adulthood. Although existing children's milk powders pay attention to basic nutrition supplementation, there are still deficiencies in terms of intelligence, intestinal health and comprehensive absorption effects. For example, most products only add DHA or a single intelligent ingredient, lacking the synergistic promotion effect on brain development and being difficult to comprehensively support the improvement of cognitive function; the protein source in traditional formulas is single, and some children have lactose intolerance or allergies, resulting in low intestinal absorption efficiency. Therefore, there is an urgent need for a children's milk powder with the characteristics of intelligence, nutrition and easy absorption. Summary of the Invention

[0003] The present invention provides a children's intelligent nutrition formula growth milk powder and its preparation method and application, which comprehensively promote the development of children's brains and vision, improve the intestinal microecology, enhance the protein absorption rate and reduce the allergy risk, and enhance the immunity and anti-fatigue ability.

[0004] The present invention provides a children's nutritional formula milk powder, comprising the following components in parts by mass: 120-135 parts of a composite protein component, 1-8 parts of a prebiotic, 15-25 parts of a sweetener, 0.2-0.3 parts of vitamins, 0.9-1.1 parts of minerals, 0.9-1.5 parts of an intelligent ingredient, 0.5 parts of an emulsifier, 0.1-0.25 parts of a nutritional supplement and 0.03-0.06 parts of a probiotic;

[0005] The intelligent ingredient includes phosphatidylserine, arachidonic acid, docosahexaenoic acid and lutein;

[0006] The composite protein component includes a combination of at least two of the following: whole milk powder, raw milk, skim milk powder, soy protein isolate, lactoferrin and hydrolyzed whey protein;

[0007] The prebiotic is selected from at least one of the following: galactooligosaccharide, isomaltooligosaccharide and fructooligosaccharide;

[0008] The probiotic includes Bifidobacterium animalis subsp. lactis.

[0009] In a preferred embodiment of the present invention, the composite protein component comprises any one of the following combinations: (1) a combination of whole milk powder, raw milk, and soy protein isolate; (2) a combination of whole milk powder, raw milk, soy protein isolate, and lactoferrin; (3) a combination of skim milk powder, whole milk powder, and soy protein isolate; (4) a combination of whole milk powder, raw milk, and hydrolyzed whey protein.

[0010] In a preferred embodiment of the present invention, among the brain-boosting components, the mass ratio of phosphatidylserine, arachidonic acid, docosahexaenoic acid, and lutein is (0.4 - 0.6):(0.25 - 0.35):(0.25 - 0.3):0.041.

[0011] In a preferred embodiment of the present invention, among the prebiotics, the mass ratio of galactooligosaccharide and isomaltooligosaccharide is (0.1 - 1):4, and the mass ratio of galactooligosaccharide and fructooligosaccharide is (0.1 - 0.5):3.

[0012] In a preferred embodiment of the present invention, the vitamins include Compound Vitamin for Nutritional Fortification of Infant Formula A and vitamin C.

[0013] In a preferred embodiment of the present invention, the nutritional supplements include L-carnitine and choline chloride.

[0014] In a preferred embodiment of the present invention, the probiotics include Bifidobacterium animalis subsp. lactis Bb-12 and / or Bifidobacterium animalis subsp. lactis HN019.

[0015] In a preferred embodiment of the present invention, the minerals include Compound Minerals for Nutritional Fortification of Infant Formula A and calcium carbonate; the composition of the Compound Minerals for Nutritional Fortification of Infant Formula A includes ferrous sulfate, zinc sulfate, sodium selenite, and lactose.

[0016] The present invention also provides a method for preparing the above-mentioned infant nutritional formula, comprising the following steps: (1) mixing the composite protein component, prebiotics, emulsifier, part of the vitamins, part of the sweeteners, and part of the minerals with water to form an emulsion;

[0017] (2) homogenizing, concentrating, and drying the emulsion to obtain a dry powder;

[0018] (3) mixing the dry powder with the brain-boosting components, nutritional supplements, probiotics, the remaining part of the vitamins, the remaining part of the sweeteners, and the remaining part of the minerals to obtain the infant nutritional formula.

[0019] The present invention also provides the application of the above-mentioned infant nutritional formula in food processing.

[0020] Beneficial effects: The present invention provides a children's nutritional formula milk powder, which contains a variety of intelligence-enhancing components, and there is a synergistic effect among docosahexaenoic acid (DHA), arachidonic acid (ARA), phosphatidylserine and lutein, comprehensively promoting the development of children's brains and vision; it contains a composite protein system with a biological value (BV) of 90% for protein, higher than that of traditional single milk protein, improving protein absorption rate and reducing the risk of allergy; the vitamin components and lutein can also produce a coordinated effect, enhancing the free radical scavenging ability and the anti-fatigue effect; at the same time, probiotics and prebiotics can also build a probiotic-prebiotic symbiotic system to improve the intestinal microecology. Through aspects such as composite intelligence-enhancing component design, protein-probiotic synergistic system, and antioxidant chain reaction optimization, the present invention systematically solves the defects of existing children's milk powder in terms of intelligence, absorption, immunity and palatability, forming multi-dimensional competitive advantages. Detailed implementation mode

[0021] The present invention provides a children's nutritional formula milk powder, which includes the following components in parts by mass: 120-135 parts of composite protein component, 1-8 parts of prebiotic, 15-25 parts of sweetener, 0.2-0.3 parts of vitamin, 0.9-1.1 parts of mineral, 0.9-1.5 parts of intelligence-enhancing component, 0.5 parts of emulsifier, 0.1-0.25 parts of nutritional supplement and 0.03-0.06 parts of probiotic;

[0022] The intelligence-enhancing component includes phosphatidylserine, arachidonic acid, docosahexaenoic acid and lutein;

[0023] The composite protein component includes a combination of at least two of the following: whole milk powder, raw milk, skim milk powder, soy protein isolate, lactoferrin and hydrolyzed whey protein;

[0024] The prebiotic is selected from at least one of the following: galactooligosaccharide, isomaltooligosaccharide and fructooligosaccharide;

[0025] The probiotic includes Bifidobacterium animalis subsp. lactis.

[0026] The mass fraction of the composite protein component in the present invention is 120-135 parts, such as 120 parts, 121 parts, 122 parts, 123 parts, 124 parts, 125 parts, 126 parts, 127 parts, 128 parts, 129 parts, 130 parts, 131 parts, 132 parts, 133 parts, 134 parts or 135 parts. Of course, it can also be other parts in decimal form, such as 130.8 parts in one embodiment, 130.85 parts in one embodiment, 120.5 parts in one embodiment, and 120.8 parts in one embodiment.

[0027] The composite protein component described in the present invention includes any one of the following combinations: (1) a combination of whole milk powder, raw milk, and soy protein isolate; (2) a combination of whole milk powder, raw milk, soy protein isolate, and lactoferrin; (3) a combination of skim milk powder, whole milk powder, and soy protein isolate; (4) a combination of whole milk powder, raw milk, and hydrolyzed whey protein. And in combination (1), the mass ratio of the whole milk powder, raw milk, and soy protein isolate is (60 - 65):(60 - 65):0.8. For example, in one embodiment, the combination is in a mass ratio of 65:65:0.8, and in another embodiment, the combination is in a mass ratio of 60:60:0.8. In combination (2), the mass ratio of the whole milk powder, raw milk, soy protein isolate, and lactoferrin can be (60 - 70):(65 - 75):(0.5 - 1.0):(0.01 - 0.1). In one embodiment, the combination is in a mass ratio of 60:70:0.8:0.05. In combination (3), the mass ratio of the skim milk powder, whole milk powder, and soy protein isolate can be (60 - 70):(60 - 70):(0.5 - 1.0). In one embodiment, the combination is in a mass ratio of 65:65:0.8. In combination (4), the mass ratio of the whole milk powder, raw milk, and hydrolyzed whey protein can be (50 - 70):(65 - 75):(0.1 - 1). In one embodiment, the combination is in a mass ratio of 50:70:0.5. In the present invention, raw milk provides essential amino acids, and soy protein isolate supplements vegetable protein, reducing the risk of lactose intolerance. After combining raw milk and soy protein isolate, the biological value (BV) of the protein can reach 90%, which is higher than that of traditional single milk protein, thereby reducing the incidence of diarrhea, improving constipation, and promoting intestinal improvement.

[0028] The mass portion of the prebiotic in the present invention is 1 - 8 parts, such as it can be 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, or 8 parts. Of course, it can also be other parts in decimal form. For example, in one embodiment, it is 2.3 parts, in one embodiment, it is 4.1 parts, and in one embodiment, it is 7.5 parts.

[0029] The prebiotic described in the present invention is a composition of at least one of galactooligosaccharide, isomaltooligosaccharide, and fructooligosaccharide. For example, a composition formed by galactooligosaccharide and isomaltooligosaccharide. And in the composition, the mass ratio of galactooligosaccharide and isomaltooligosaccharide can be (0.1 - 1):(2 - 4). For example, in one embodiment, it is 0.1:4. It can also be a composition formed by galactooligosaccharide and fructooligosaccharide, and the mass ratio of galactooligosaccharide and fructooligosaccharide can be 0.1:2. And it can also be a composition formed by galactooligosaccharide, isomaltooligosaccharide, and fructooligosaccharide. In one embodiment, the mass ratio of galactooligosaccharide, isomaltooligosaccharide, and fructooligosaccharide is 0.5:4:3.

[0030] The mass portion of the intelligence-enhancing component in the present invention is 0.9 to 1.5 portions, which can be 0.9 portion, 1 portion, 1.1 portions, 1.2 portions, 1.3 portions, 1.4 portions or 1.5 portions. Of course, it can also be other portions in decimal form, such as 0.941 portion in one embodiment, 1.241 portion in one embodiment, and 1.141 portion in one embodiment. The intelligence-enhancing component in the present invention is a quaternary composition, including phosphatidylserine, arachidonic acid (ARA), docosahexaenoic acid (DHA) and lutein, and the mass ratio of phosphatidylserine, ARA, DHA and lutein is (0.4 to 0.6):(0.25 to 0.35):(0.25 to 0.3):0.041, which is 0.4:0.25:0.25:0.041 in one embodiment, 0.5:0.3:0.3:0.041 in one embodiment, 0.4:0.35:0.35:0.041 in one embodiment, 0.6:0.25:0.25:0.041 in one embodiment, and 0.6:0.35:0.25:0.041 in one embodiment. The intelligence-enhancing component in the present invention, through the synergistic effects of DHA (neuron growth), ARA (brain cell membrane synthesis), phosphatidylserine (memory enhancement) and lutein (visual protection), covers the entire link of brain development and can play a synergistic effect, comprehensively improving children's cognitive ability; phosphatidylserine promotes neurotransmitter transmission, and lutein protects the retina, enhancing the neuroprotective ability and avoiding the limitations of traditional formulations that only rely on DHA. The mass ratio of DHA and ARA in the present invention meets the requirements of the fatty acid composition of children's brain tissue, avoiding the metabolic burden caused by excessive single components.

[0031] The mass portion of the probiotic in the present invention is 0.03 to 0.06 portions, which can be 0.03 portion, 0.04 portion, 0.05 portion and 0.06 portion. The probiotic in the present invention belongs to Bifidobacterium animalis subsp. lactis, such as it can be Bifidobacterium animalis subsp. lactis Bb-12 and / or HN019. In some embodiments, it only contains Bb-12, and its mass portions are 0.03 portion and 0.05 portion. In one embodiment, it contains Bb-12 and HN019, and their mass portions are 0.03 portion and 0.03 portion respectively. The probiotic in the present invention can form a symbiotic system with prebiotics, such as the probiotic can be combined with prebiotics to promote the proliferation of Bifidobacterium.

[0032] The mass parts of the sweetener in the present invention are 15 to 25 parts, such as 15 parts, 16 parts, 17 parts, 18 parts, 19 parts, 20 parts, 21 parts, 22 parts, 23 parts, 24 parts and 25 parts. Of course, it can also be other parts in decimal form, such as the weight parts in the examples are 15 parts, 16 parts, 18.5 parts and 25 parts respectively. The sweetener in the present invention is selected from at least one of the following: crystalline fructose, corn syrup and maltodextrin. In some embodiments, it is a combination of crystalline fructose and corn syrup, and the mass ratio of the crystalline fructose and the corn syrup can be (5 to 6):(10 to 12.5), such as 6:12.5, 6:10, 5:10 and 6:12.5; in one embodiment, it is a combination of maltodextrin and corn syrup, and its mass ratio is 10:15. In the present invention, crystalline fructose and corn syrup are compounded, and the sweet taste is soft. At the same time, the compounding of crystalline fructose (low GI) and solid corn syrup (viscosity adjustment) can balance solubility and taste, make the brewing time ≤ 15 seconds, and there is no caking.

[0033] The mass parts of the vitamin in the present invention are 0.235 parts, and the vitamin includes a compounded vitamin A for fortified compounded children's formula milk powder and vitamin C, and the mass ratio of the two is 0.15:0.085. The addition of the vitamin C in the present invention can directly scavenge free radicals, lutein protects cell membranes from oxidative damage, vitamin C and lutein produce a synergistic effect, synergistically enhance the antioxidant chain reaction, improve the free radical scavenging ability, and enhance the anti-fatigue effect and immunity.

[0034] The compounded vitamin A for fortified compounded children's formula milk powder in the present invention, calculated according to the dilution ratio of the diluent: vitamin A (325,000 IU) (vitamin A (retinyl acetate) 11.50%, white granulated sugar 30%, corn starch 36.50%, arabic gum 20%, dl-α-tocopherol 1.50%, tricalcium phosphate 0.50%); vitamin D3 (100,000 IU) (vitamin D3 (cholecalciferol) 0.25%, white granulated sugar 40%, arabic gum 30%, corn starch 18.15%, medium-chain triglycerides 11%, tricalcium phosphate 0.5%, dl-α-tocopherol 0.10%); vitamin E (50%) (vitamin E (dl-α-tocopheryl acetate 50%, sodium starch octenyl succinate 50.0%); vitamin K1 (5%) (phytomenadione 5%, citric acid fatty acid glyceride 20.00%, maltodextrin 75.00%); vitamin B1 (thiamine hydrochloride 100%); vitamin B2 (10%) (riboflavin 10.00%, citric acid 0.19%, sodium citrate 2.650%, maltodextrin 87.160%); vitamin B6 (5%) (pyridoxine hydrochloride 5.00%, citric acid 0.27%, sodium citrate 2.530%, maltodextrin 92.200%); vitamin B 12(1%) (cyanocobalamin 1.00%, citric acid 0.79%, sodium citrate 1.740%, maltodextrin 96.470%); biotin (1%) (D-biotin 1%, citric acid 0.45%, sodium citrate 2.25%, maltodextrin 96.30%); niacinamide (niacinamide 100%); folic acid (10%) (folic acid 10.0%, citric acid 0.19%, sodium citrate 2.65%, maltodextrin 87.16%); calcium D-pantothenate (calcium D-pantothenate 100%); taurine (taurine 100%); lactose (lactose 100%) are compounded. In the embodiment of the present invention, the compounded vitamin A for fortified children's formula milk powder used is purchased from Beijing Jinkangpu Food Technology Co., Ltd.

[0035] The mass portion of the mineral in the present invention is 0.9 - 1.1 portions, such as 0.9 portion, 1.0 portion and 1.1 portion. The mineral in the present invention is a complex of the compounded mineral A for fortified children's formula milk powder and calcium carbonate, and the mass ratio of the compounded mineral A for fortified children's formula milk powder to calcium carbonate is 0.1:(0.8 - 1.0), such as 0.1:0.8 in some embodiments and 0.1:1 in other embodiments. The composition of the compounded mineral A for fortified children's formula milk powder in the present invention includes ferrous sulfate, zinc sulfate, sodium selenite and lactose. In one embodiment, calculated by the dilution ratio, the compounded children's complex mineral A is compounded from the following components by mass percentage: ferrous sulfate 79.2%, zinc sulfate (zinc sulfate monohydrate 100%), sodium selenite 0.5% and lactose (lactose 100%), wherein ferrous sulfate is composed of 90.5% (m / m) of dry ferrous sulfate and 9.5% (m / m) of citric acid; zinc sulfate is zinc sulfate monohydrate 100%; sodium selenite is composed of 0.50% (m / m) of sodium selenite, 0.003% (m / m) of citric acid and 99.497% (m / m) of maltodextrin. The compounded mineral A for fortified children's formula milk powder used in the embodiment of the present invention is purchased from Beijing Jinkangpu Food Technology Co., Ltd.

[0036] The mass portion of the emulsifier in the present invention is 0.5 portion, and the emulsifier can be mono- and diglycerol fatty acid esters.

[0037] The mass fraction of the nutritional supplement in the present invention is 0.11 to 0.21 parts, such as 0.11 part, 0.12 parts, 0.13 parts, 0.14 parts, 0.15 parts, 0.16 parts, 0.17 parts, 0.18 parts, 0.19 parts, 0.20 parts and 0.21 parts. The nutritional supplement in the present invention includes L-carnitine and choline chloride, and the mass ratio of L-carnitine to choline chloride is (0.01 - 0.1):(0.1 - 0.2), such as 0.01:0.1 in one embodiment, 0.01:0.15 in one embodiment, 0.01:0.2 in one embodiment, and 0.05:0.1 in one embodiment. The L-carnitine and choline chloride in the present invention, as nutritional supplements, respectively provide carnitine and choline.

[0038] The addition of various raw materials in the formula of the present invention needs to follow various standards and be added within the standard range.

[0039] Table 1 Sources, functions and addition bases of raw materials in the formula of the present invention

[0040]

[0041]

[0042] The present invention also provides a preparation method of the above-mentioned children's nutritional formula milk powder, which includes the following steps: (1) Mix the composite protein component, prebiotic, emulsifier, part of vitamins, part of sweeteners and part of minerals with water to form an emulsion;

[0043] (2) Homogenize, concentrate and dry the emulsion to obtain dry powder;

[0044] (3) Mix the dry powder with the intelligence-enhancing component, nutritional supplement, probiotic, the remaining part of vitamins, the remaining part of sweeteners and the remaining part of minerals to obtain the children's total nutritional formula milk powder.

[0045] The preparation method of the present invention is a dry-wet composite production process. First, a composite protein component, prebiotics, emulsifiers, some vitamins, some sweeteners, and some minerals are mixed with water to form an emulsion. For example, in one embodiment, whole milk powder, skim milk powder, raw cow milk, and soy protein isolate are mixed with water by a powder-water mixer to obtain a whole milk powder mixture, and the water used is purified water at 65°C. In addition, the compound vitamins are separately dissolved in water. The present invention adds weighed galactooligosaccharides, crystalline fructose, and isomaltooligosaccharides to the already mixed whole milk powder mixture, and shears to obtain Emulsion 1. Separately dissolved (dissolution temperature 40°C) children's compound vitamin A and children's compound mineral A are added to Emulsion 1 and sheared for 15 minutes to obtain Emulsion 2. The sheared Emulsion 2 is made up to a powder-water mixing ratio of 1:5 with pure water at 65°C, homogenized through a homogenizer, then sterilized at high temperature, and then concentrated and spray-dried to obtain dry powder. In one embodiment of the present invention, the parameters of the spray drying mainly include the following: the negative pressure in the tower is about -30 to -150 Pa. The exhaust air temperature reaches 85 to 95°C, the inlet air temperature is 160 to 200°C, the spray gun is turned on to control the tower temperature between 85 and 95°C, the pressure of the high-pressure pipeline is adjusted between 160 and 220 bar, and the exhaust air temperature is slightly adjusted according to the moisture content of the powder after powder discharge. The material enters the tower in a mist state through the spray gun, exchanges heat with hot air, quickly evaporates water, becomes powdery, and falls to the fixed fluidized bed at the bottom of the tower. The inlet air temperature of the fixed fluidized bed is controlled at 55 to 70°C. When powder is discharged from the tower, the powder moves forward evenly in a suspended state, and the powder is further dried and cooled during the movement. The main process parameters are as follows: the inlet air temperature of the first stage of the vibrating fluidized bed: 30 to 50°C; the inlet air temperature of the second stage of the vibrating fluidized bed: 15 to 30°C; the inlet air temperature of the third stage of the vibrating fluidized bed: 13 to 18°C; the powder temperature ≤ 35°C.

[0046] The present invention mixes the dry powder, phosphatidylserine, compound vitamin C nutritional fortifier, arachidonic acid oil powder (10%), docosahexaenoic acid oil powder (fermentation method, 10%), compound lutein (0.5%) nutritional fortifier, and Bifidobacterium animalis subsp. lactis to obtain the product. When mixing, 10% of the spray-dried dry powder is used for primary mixing, the mixing time is 15 minutes, and the mixing frequency is 40 HZ. 80% of the spray-dried dry powder is used to mix with the powder obtained from the primary mixing for secondary mixing, the mixing time is 15 minutes, and the mixing frequency is 40 HZ. The powder obtained from the secondary mixing is mixed with the remaining spray-dried dry powder, the mixing time is 20 minutes, and the mixing frequency is 40 HZ.

[0047] The present invention also provides the application of the above-mentioned children's nutritional formula milk powder in food processing.

[0048] To further illustrate the present invention, a children's intelligent nutritional formula growth milk powder provided by the present invention, its preparation method and application will be described in detail below in conjunction with embodiments, but they cannot be construed as limiting the protection scope of the present invention.

[0049] Example 1

[0050] A children's intelligent nutritional formula growth milk powder, the raw materials of which include the following components in percentage by weight: 65 parts of whole milk powder, 65 parts of raw cow milk, 0.8 part of soy protein isolate, 0.1 part of galactooligosaccharide, 6 parts of crystalline fructose, 4 parts of isomaltooligosaccharide, 0.15 part of compound children's formula milk powder nutritional fortifier A compound vitamin, 0.1 part of compound children's formula milk powder nutritional fortifier A compound mineral, 0.4 part of phosphatidylserine, 0.085 part of compound vitamin C nutritional fortifier, 0.8 part of calcium carbonate, 0.5 part of mono- and diglycerol fatty acid esters, 0.25 part of arachidonic acid oil powder (10%), 12.5 parts of solid corn syrup, 0.25 part of docosahexaenoic acid oil powder (fermentation method, 10%), 0.01 part of L-carnitine, 0.1 part of choline chloride, 0.041 part of compound lutein (0.5%) nutritional fortifier, 0.03 part of Bifidobacterium animalis subsp. lactis Bb-12.

[0051] Example 2

[0052] A children's intelligent nutritional formula growth milk powder, the raw materials of which include the following components in percentage by weight: 60 parts of whole milk powder, 70 parts of raw cow milk, 0.8 part of soy protein isolate, 0.5 part of galactooligosaccharide, 3 parts of oligofructose, 6 parts of crystalline fructose, 4 parts of isomaltooligosaccharide, 0.15 part of compound children's formula milk powder nutritional fortifier A compound vitamin, 0.1 part of compound children's formula milk powder nutritional fortifier A compound mineral, 0.5 part of phosphatidylserine, 0.08 part of compound vitamin C nutritional fortifier, 0.8 part of calcium carbonate, 0.5 part of mono- and diglycerol fatty acid esters, 0.3 part of arachidonic acid oil powder (10%), 10 parts of solid corn syrup, 0.3 part of docosahexaenoic acid oil powder (fermentation method, 10%), 0.05 part of lactoferrin, 0.01 part of L-carnitine, 0.15 part of choline chloride, 0.041 part of compound lutein (0.5%) nutritional fortifier, 0.05 part of Bifidobacterium animalis subsp. lactis Bb-12.

[0053] Example 3

[0054] A growth milk powder with a children's intellectual development and nutrition formula, the raw materials of which, by weight percentage, include the following components: 65 parts of skim milk powder, 65 parts of whole milk powder, 0.8 part of soy protein isolate, 0.3 part of galactooligosaccharide, 5 parts of crystalline fructose, 2 parts of fructooligosaccharide, 0.15 part of compound vitamin for children's formula milk powder nutritional fortifier A, 0.1 part of compound mineral for children's formula milk powder nutritional fortifier A, 0.4 part of phosphatidylserine, 0.085 part of compound vitamin C nutritional fortifier, 0.8 part of calcium carbonate, 0.5 part of mono- and diglycerol fatty acid esters, 0.35 part of arachidonic acid oil powder (10%), 10 parts of solid corn syrup, 0.35 part of docosahexaenoic acid oil powder (fermentation method, 10%), 0.01 part of L-carnitine, 0.1 part of choline chloride, 0.041 part of compound lutein (0.5%) nutritional fortifier, 0.03 part of Bifidobacterium animalis subsp. lactis Bb-12, 0.03 part of Bifidobacterium animalis subsp. lactis HN019.

[0055] Example 4

[0056] A growth milk powder with a children's intellectual development and nutrition formula, the raw materials of which, by weight percentage, include the following components: 50 parts of whole milk powder, 70 parts of raw cow milk, 5.0 parts of hydrolyzed whey protein powder, 0.1 part of galactooligosaccharide, 6 parts of crystalline fructose, 4 parts of isomaltooligosaccharide, 0.15 part of compound vitamin for children's formula milk powder nutritional fortifier A, 0.1 part of compound mineral for children's formula milk powder nutritional fortifier A, 0.6 part of phosphatidylserine, 0.085 part of compound vitamin C nutritional fortifier, 1.0 part of calcium carbonate, 0.5 part of mono- and diglycerol fatty acid esters, 0.25 part of arachidonic acid oil powder (10%), 12.5 parts of solid corn syrup, 0.25 part of docosahexaenoic acid oil powder (fermentation method, 10%), 0.01 part of L-carnitine, 0.2 part of choline chloride, 0.041 part of compound lutein (0.5%) nutritional fortifier, 0.03 part of Bifidobacterium animalis subsp. lactis Bb-12.

[0057] Example 5

[0058] A children's intelligent nutritional formula growth milk powder, the raw materials of which include the following components in weight percentages: 60 parts of whole milk powder, 60 parts of raw milk, 0.8 part of soy protein isolate, 0.1 part of galactooligosaccharide, 10 parts of maltodextrin, 4 parts of isomaltooligosaccharide, 0.15 part of compound vitamin for children's formula milk powder nutritional fortifier A, 0.1 part of compound mineral for children's formula milk powder nutritional fortifier A, 0.6 part of phosphatidylserine, 0.085 part of compound vitamin C nutritional fortifier, 1.0 part of calcium carbonate, 0.5 part of mono- and diglycerol fatty acid esters, 0.35 part of arachidonic acid oil powder (10%), 15 parts of solid corn syrup, 0.25 part of docosahexaenoic acid oil powder (fermentation method, 10%), 0.05 part of L-carnitine, 0.1 part of choline chloride, 0.041 part of compound lutein (0.5%) nutritional fortifier, 0.03 part of Bifidobacterium animalis subsp. lactis Bb-12.

[0059] The preparation of the children's intelligent nutritional formula growth milk powder in Examples 1-5 all adopts the dry-wet composite process, which specifically includes the following steps:

[0060] (1) Raw material weighing: Raw materials such as whole milk powder, skim milk powder, raw milk, hydrolyzed whey protein powder, soy protein isolate, galactooligosaccharide, crystalline fructose, isomaltooligosaccharide, compound vitamin for children's formula milk powder nutritional fortifier A, compound mineral for children's formula milk powder nutritional fortifier A, etc.

[0061] (2) Raw material mixing: Mix whole milk powder, skim milk powder, raw milk, and soy protein isolate with water through a powder-water mixer to obtain a whole milk powder mixture. The water used is purified water at 65°C. In addition, the compound vitamin is dissolved separately in water.

[0062] (3) Shearing: Add the weighed galactooligosaccharide, crystalline fructose, and isomaltooligosaccharide to the already mixed whole milk powder mixture, shear to obtain Emulsion 1. In addition, add the compound vitamin for children's formula milk powder nutritional fortifier A and the compound mineral for children's formula milk powder nutritional fortifier A that are separately dissolved (dissolution temperature 40°C) to Emulsion 1, and shear for 15 minutes to obtain Emulsion 2.

[0063] (4) Homogenization and concentration: Make the sheared Emulsion 2 up to a powder-water mixing ratio of 1:5 with pure water at 65°C, homogenize the emulsion through a homogenizer, then sterilize it at high temperature, and then concentrate.

[0064] (5) Obtain dry powder by spray drying: The negative pressure inside the tower is around -30 to -150 Pa. The exhaust air temperature reaches 85 to 95 °C, the inlet air temperature is 160 to 200 °C. Turn on the spray gun to control the tower temperature between 85 and 95 °C, adjust the pressure of the high-pressure pipeline between 160 and 220 bar, and make fine adjustments to the exhaust air temperature according to the moisture content of the powder after the powder is discharged. The material enters the tower in a mist form through the spray gun, undergoes heat exchange after contacting the hot air, quickly evaporates the moisture, becomes powdery, and falls to the fixed fluidized bed at the bottom of the tower. The inlet air temperature of the fixed fluidized bed is controlled between 55 and 70 °C. When the powder is discharged from the tower, the powder moves forward evenly in a suspended state, and the powder is further dried and cooled during the movement. The main process parameters are as follows: the inlet air temperature of the first stage of the vibrating fluidized bed: 30 to 50 °C; the inlet air temperature of the second stage of the vibrating fluidized bed: 15 to 30 °C; the inlet air temperature of the third stage of the vibrating fluidized bed: 13 to 18 °C; the powder temperature ≤ 35 °C.

[0065] (6) Mix the dry powder, phosphatidylserine, compound vitamin C nutritional fortifier, arachidonic acid oil powder (10%), docosahexaenoic acid oil powder (fermentation method, 10%), compound lutein (0.5%) nutritional fortifier, and Bifidobacterium animalis subsp. lactis to obtain the product. When mixing, use 10% of the spray-dried dry powder for primary mixing, the mixing time is 15 minutes, and the mixing frequency is 40 HZ. Use 80% of the spray-dried dry powder to mix with the powder from the primary mixing for secondary mixing, the mixing time is 15 minutes, and the mixing frequency is 40 HZ. Use the powder from the secondary mixing to mix with the remaining spray-dried dry powder, the mixing time is 20 minutes, and the mixing frequency is 40 HZ.

[0066] Comparative Example 1: The difference from Example 1 is that only DHA + ARA is retained, and phosphatidylserine and lutein are deleted.

[0067] Compare the effects of Example 1 and Comparative Document 1:

[0068] 1. Conduct behavioral tests according to the reference (Wang Li, Guo Yueyi, Zhao Jian, etc. Study on the improvement of memory function in mice by whey protein powder rich in phospholipids [J]. Journal of Food Safety and Quality, 2022, 13(14): 9.). The new object recognition index of Example 1 is 79.4%, that of Comparative Example 1 is 58.3%, and the memory improvement amplitude decreases by 35% (P>0.01).

[0069] 2. Molecular mechanism: Western Blot analysis was performed according to the reference (Guo Yueyi, Guo Yu, Zhao Jian, et al. Study on the improvement of memory function in mice by compound whey protein powder [J]. China Dairy Industry, 2022, 50(12): 10-16. DOI: 10.19827 / j.issn1001-2230.2022.12.002.). The expression level of PSD95 protein in the hippocampus of Comparative Example 1 was 48% lower than that of Example 1, the lutein content in the retina was not detected, and the oxidative damage index (MDA) increased by 37%.

[0070] Comparative Example 2: The difference from Example 2 is that lactoferrin was deleted, the probiotic content was reduced to 0.2 parts, and the galactooligosaccharide content was reduced to 0.1 part.

[0071] According to the method described in the reference (Jiang Hui, Du Guangcai. Effects of the combined application of astaxanthin, docosahexaenoic acid, and phosphatidylserine on memory and immunity in a mouse aging model [J]. Light Industry Science and Technology, 2020(3): 3. DOI: CNKI:SUN:GXQG.0.2020-03-045.), the effects of Example 2 and Comparative Example 2 were compared:

[0072] 1. Immune index: After ELISA detection, the serum IgA level was 42% lower than that of Example 2; after fecal detection, the intestinal sIgA secretion decreased by 55%.

[0073] 2. Bacterial colonization: After 16S rRNA sequencing, the abundance of Bifidobacterium was only 30% of that of Example 2.

[0074] Comparative Example 3: The difference from Example 3 is that the dual probiotics were adjusted to only 0.03 parts of Bifidobacterium animalis subsp. lactis Bb-12, 2.0 parts of fructooligosaccharide were deleted, and at the same time, the crystalline fructose was increased to 7 parts.

[0075] According to the method described in the reference (Jiang Hui, Du Guangcai. Effects of the combined application of astaxanthin, docosahexaenoic acid, and phosphatidylserine on memory and immunity in a mouse aging model [J]. Light Industry Science and Technology, 2020(3): 3. DOI: CNKI:SUN:GXQG.0.2020-03-045.), the effects of Example 3 and Comparative Example 3 were compared:

[0076] 1. Cognitive correlation: The correct rate of the Y maze in Example 3 was 85.6%, while that in the comparative example was only 68.5%.

[0077] 2. Intestinal function: The intestinal permeability (serum DAO activity) in Comparative Example 3 was 2.8 times higher than that in Example 3. After GC-MS detection, the total amount of short-chain fatty acids (SCFA) decreased by 62%.

[0078] Comparative Example 4: The difference from Example 4 is that the hydrolyzed whey protein is adjusted to ordinary whey protein, and at the same time, the total amount of DHA and ARA is reduced from 0.35 parts to 0.25 parts. When the addition amount is 0.35 parts, there is an obvious fishy smell, and the product flavor can be improved after the reduction.

[0079] Compare the effects of Example 4 and Comparative Example 4:

[0080] 1. Growth index: The growth rate of the body weight of the young mice is 25% lower than that of Example 4 (P<0.05), and the femoral length is reduced by 8% (Micro-CT scan).

[0081] 2. Protein absorption: The total amount of plasma essential amino acids (EAA) decreases by 33% (HPLC detection).

[0082] Comparative Example 5: The difference from Example 5 is that the content of L-carnitine is reduced to 0.01 part, and at the same time, maltodextrin is replaced with glucose syrup.

[0083] Compare the effects of Example 5 and Comparative Example 5:

[0084] 1. Energy metabolism: The fluctuation range of postprandial blood glucose is 40% higher than that of Example 5 (area under the OGTT curve), and the fat oxidation rate decreases by 28% (indirect calorimetry).

[0085] 2. Physical performance: The exercise endurance (treadmill time) is only 65% of that of Example 5 (P<0.01).

[0086] Effect Example 1 Sensory Evaluation

[0087] (1) Color and luster: Take 20 g each of the children's intelligent whole-nutrition formula growth milk powder obtained in Examples 1 to 5 and Comparative Examples 1 to 5 and the commercially available product A and place them on weighing paper or a white porcelain plate, observe their color and luster, tissue state, and smell their flavor.

[0088] (2) Tissue state: Under the incandescent light source in the sensory evaluation laboratory, take 40 g each of the children's intelligent whole-nutrition formula growth milk powder obtained in Examples 1 - 5 and Comparative Examples 1 - 5 and the commercially available product A as the samples to be tested and place them on weighing paper or a white porcelain plate. Observe the tissue state of the milk powder with the naked eye to see if there are lumps, scorched powder, or foreign objects.

[0089] (3) Reconstitution effect: Take 30.5 g each of the children's intelligent whole-nutrition formula growth milk powder obtained in Examples 1 to 5 and Comparative Examples 1 to 5 and the commercially available product A as the samples and pour them into a beaker containing 210 mL of distilled water at 45°C ± 2°C. Stir manually with a glass rod 15 circles to the left and 15 circles to the right, and then let it stand for 3 minutes. Observe the state of the reconstituted liquid. The results of Effect Example 1 are shown in Table 2. Compared with the comparative examples and the commercially available product A, the children's intelligent nutrition formula growth milk powder obtained in Examples 1 to 5 has good sensory evaluation, good solubility, and no white spots or lumps.

[0090] Table 2 Sensory Evaluation Results Table

[0091]

[0092]

[0093] Meanwhile, 23 healthy people aged 3 - 14 years old were randomly selected to score and evaluate Examples 1 - 5, Comparative Examples 1 - 5 and commercially available product A in terms of appearance, fragrance, flavor, sweetness, milk flavor, taste, aftertaste and overall in the form of blind samples. The evaluation results are shown in Table 3, and the overall preference evaluations of Examples 1 - 5 are higher than those of commercially available product A and Comparative Examples 1 - 5.

[0094] Table 3 Scoring Evaluation Table

[0095] Appearance Fragrance Flavor Sweetness Milk flavor Texture Aftertaste Overall Example 1 7.25 8.15 7.25 7.05 7.05 7.85 7.15 7.75 Example 2 7.00 7.00 6.15 6.80 7.20 7.15 7.00 7.35 Example 3 7.30 7.20 7.10 6.80 6.65 7.30 7.25 7.25 Example 4 7.05 8.10 7.05 7.00 7.35 7.85 7.10 7.05 Example 5 7.00 7.10 6.35 6.50 7.00 7.15 7.20 7.25 Comparative Example 1 6.30 6.30 7.00 6.60 6.95 7.10 7.15 7.00 Comparative Example 2 6.35 7.75 7.00 6.15 6.35 6.05 7.00 7.05 Comparative Example 3 7.00 6.50 6.15 6.80 7.20 7.15 7.00 6.75 Comparative Example 4 7.30 7.20 7.10 6.80 6.65 7.30 7.25 6.55 Comparative Example 5 7.05 7.15 7.05 7.05 6.35 6.85 7.10 7.05 Product A 7.00 6.80 6.35 6.50 6.25 7.15 7.20 6.70

[0096] Study on the Memory Ability and Nutrition of Mice in Effect Example 2

[0097] 80 Kunming white mice were selected and purchased from Shandong Pabeile White Mouse Breeding Cooperative. They were adaptively fed for 1 week with free diet and water. Then, they were randomly divided into 8 groups with 10 mice in each group (20 ± 2 g). The 8 groups of mice were the normal group, Example 1 group, Example 2 group, Example 3 group, Comparative Example 3 group, Comparative Example 4 group, Comparative Example 5 group, and commercially available product A group. Examples 1 - 3 were fed with the milk powders of Examples 1 - 3, Comparative Examples 3 - 5 were fed with the milk powders of Comparative Examples 3 - 5, the normal group was fed with conventional feed, and the commercially available product A group was fed with the milk powder of commercially available product A. The 8 groups of white mice were continuously fed with the milk powder at a brewing concentration of 12.5%. The test dose for the white mice was 5 mL / time. The groups of Examples 1 - 3, Comparative Examples 3 - 5 and commercially available product A group were fed with the corresponding milk powders in the morning, noon and evening, and continuously gavaged for 30 days with free drinking water; the normal group was gavaged with the same dose of pure water. After the last feeding, each group was weighed, and the step - down test and mouse nutrition research experiment were respectively carried out.

[0098] Step - down test: The white mice were placed in the reaction box to freely explore the environment for 3 min to get familiar with the environment. Subsequently, a 36V alternating current was applied for stimulation. When the mice's four limbs touched the bottom fence, they received an electric shock and instinctively jumped onto the insulating platform. Record the time from being placed in the reaction box to the first time jumping off the platform (latency), the number of times of jumping off the platform within 5 min (each time the four limbs touch the fence is counted as 1 error), and the number of animals jumping off the platform in each group (memory acquisition). After 24 h, the test was repeated, and record the latency of the first time jumping off the platform, the number of errors of jumping off the platform within 3 min, and the number of animals jumping off the platform in each group (memory consolidation). If the mice did not jump off the platform within 5 min, the latency was recorded as 300 seconds and the number of errors was 0.

[0099] Mouse nutrition research experiment: After the step-down test was completed, the mice were anesthetized, and blood was taken by eye enucleation to detect the activities of SOD and CAT enzymes in the serum. The liver, spleen, and kidneys were dissected and weighed, and the organ-body ratio was calculated. The test results are shown in Tables 4 and 5 respectively.

[0100] Table 4 Mouse memory ability

[0101]

[0102] Memory acquisition: The latency periods of the mice in Example Groups 1-3 were shortened by about 16-23% compared with the normal group and by about 10% compared with Group A of the commercially available product. The number of errors decreased by about 18-28% compared with the normal group and by about 9% compared with Group A of the commercially available product.

[0103] Memory consolidation: The latency period of the Example Group was extended to 3 times that of the normal group, and the number of errors decreased by 82%, which was better than that of the Comparative Example Group and Group A of the commercially available product. According to the results of the mouse step-down test, it can be seen that feeding the children's intelligent full-nutrition formula growth milk powder of the present invention can significantly improve the memory acquisition and memory consolidation abilities of mice. Based on this, it is inferred that the milk powder of the present invention can promote the brain development of mice and improve the memory of mice;

[0104] After removing the intelligent ingredients, the memory acquisition and memory consolidation abilities of the mice were close to those of the normal group.

[0105] Table 5 Mouse nutrition research results

[0106]

[0107]

[0108] Note: Different letters in the same column indicate significant statistical differences between the corresponding groups, p < 0.05.

[0109] As can be seen from Table 5, the formulas of Examples 1-3 of the present invention can improve the organ-body ratios of the liver, spleen, and kidneys of mice and have the effect of enhancing immunity. In addition, the determination results of the activities of SOD and CAT enzymes in the serum of mice show that the formula powder of the Examples of the present invention can well scavenge the peroxide free radicals in the body and has obvious antioxidant and anti-fatigue effects compared with the model group.

[0110] Although the above embodiments have described the present invention in detail, they are only a part of the embodiments of the present invention, not all of them. People can also obtain other embodiments based on these embodiments without creative efforts, and these embodiments all belong to the protection scope of the present invention.

Claims

1. A children's nutritional formula milk powder, characterized in that, Comprising the following components in parts by mass: 120 - 135 parts of composite protein component, 1 - 8 parts of prebiotic, 15 - 25 parts of sweetener, 0.2 - 0.3 parts of vitamin, 0.9 - 1.1 parts of mineral, 0.9 - 1.5 parts of brain-boosting ingredient, 0.5 parts of emulsifier, 0.1 - 0.25 parts of nutritional supplement, and 0.03 - 0.06 parts of probiotic; The brain-boosting ingredient includes phosphatidylserine, arachidonic acid, docosahexaenoic acid, and lutein; The composite protein component includes a combination of at least two of the following: whole milk powder, raw cow milk, skim milk powder, soy protein isolate, lactoferrin, and hydrolyzed whey protein; The prebiotic is selected from at least one of the following: galactooligosaccharide, isomaltooligosaccharide, and fructooligosaccharide; The probiotic includes Bifidobacterium animalis subsp. lactis.

2. The children's nutritional formula milk powder according to claim 1, wherein The composite protein component includes any one of the following combinations: (1) a combination of whole milk powder, raw cow milk, and soy protein isolate; (2) a combination of whole milk powder, raw cow milk, soy protein isolate, and lactoferrin; (3) a combination of skim milk powder, whole milk powder, and soy protein isolate; (4) a combination of whole milk powder, raw cow milk, and hydrolyzed whey protein.

3. The children's nutritional formula milk powder according to claim 1, characterized in that Among the brain-boosting ingredients, the mass ratio of phosphatidylserine, arachidonic acid, docosahexaenoic acid, and lutein is (0.4 - 0.6) : (0.25 - 0.35) : (0.25 - 0.3) : 0.

041.

4. The children's nutritional formula milk powder according to claim 1, wherein Among the prebiotics, the mass ratio of galactooligosaccharide and isomaltooligosaccharide is (0.1 - 1) : 4, and the mass ratio of galactooligosaccharide and fructooligosaccharide is (0.1 - 0.5) :

3.

5. The children's nutritional formula milk powder according to claim 1, wherein The vitamin includes compound children's formula milk powder nutritional fortifier A compound vitamin and vitamin C.

6. The children's nutritional formula milk powder according to claim 1, wherein The nutritional supplement includes L-carnitine and choline chloride.

7. The children's nutritional formula milk powder according to claim 1, characterized in that, The probiotic includes Bifidobacterium animalis subsp. lactis Bb-12 and / or Bifidobacterium animalis subsp. lactis HN019.

8. The children's nutritional formula milk powder according to claim 1, characterized in that The mineral includes compound children's formula milk powder nutritional fortifier A compound mineral and calcium carbonate; The composition of the compound children's formula milk powder nutritional fortifier A compound mineral includes ferrous sulfate, zinc sulfate, sodium selenite, and lactose.

9. The preparation method of the children's total-nutrition formula milk powder according to any one of claims 1 to 8, characterized in that, Including the following steps: (1) Mix the composite protein component, prebiotic, emulsifier, part of the vitamin, part of the sweetener, and part of the mineral with water to form an emulsion; (2) Homogenize, concentrate, and dry the emulsion to obtain a dry powder; (3) Mix the dry powder with the brain-boosting ingredient, nutritional supplement, probiotic, the remaining part of the vitamin, the remaining part of the sweetener, and the remaining part of the mineral to obtain the children's nutritional formula milk powder.

10. Use of the children's nutritional formula milk powder according to any one of claims 1 - 8 in food processing.

Images