Nicotinamide microcapsule as well as preparation method and application thereof

Abstract

The invention relates to the technical field of biology, in particular to a nicotinamide microcapsule and a preparation method and application thereof. The preparation method of the nicotinamide microcapsule comprises the following steps: mixing gelatin and water, and heating to obtain a gelatin solution; sequentially dissolving L-alpha-choline alfoscerate, sodium alginate, isomaltooligosacharide, nicotinamide and vitamin C in the gelatin solution under the condition of constant temperature of 38-45 DEG C to obtain a mixed solution; and adjusting the pH value of the mixed solution, and continuously stirring to obtain the nicotinamide microcapsule. The nicotinamide microcapsule is good in embedding effect, high in embedding rate and good in nicotinamide embedding capacity, the absorption efficiency of the nicotinamide can be effectively improved, and the nicotinamide can be converted into more NAD < + >, so that more ATP is generated, and the bioavailability of the nicotinamide is improved.

Description

Technical Field

[0001] The present invention relates to the field of biotechnology, and particularly to a nicotinamide microcapsule, a preparation method thereof, and uses thereof. Background Art

[0002] With the surging demand for anti-aging, cell-level anti-aging has become a trend. Nicotinamide (NAM) is a B vitamin, which has the effects of enhancing skin barrier function, inhibiting melanin production, and reducing inflammatory responses, showing great application potential in the anti-aging field. Research shows that nicotinamide (NAM) is an important precursor of NAD + (nicotinamide adenine dinucleotide), which can be converted into NAD + ,and the increase in NAD + level can effectively promote ATP production, thereby enhancing cellular energy metabolism. However, as a bioactive substance, NAM has poor stability and is easily affected by enzymatic hydrolysis and gastric acid environment during in vivo metabolism, resulting in its decomposition or degradation and loss of activity, leading to poor bioavailability.

[0003] Currently, the prior art mainly uses microencapsulation embedding technology to protect the activity of nicotinamide. This microencapsulation embedding technology prepares by embedding nicotinamide in microcapsules using physical processes such as spray drying and coagulation bath. However, this method has obvious limitations: the embedding materials prepared under high temperature or high pressure environments show instability, which may cause premature release of nicotinamide and reduce its bioavailability.

[0004] Therefore, how to develop a method to improve the stability of nicotinamide has become an urgent technical problem to be solved. Summary of the Invention

[0005] The present invention provides a preparation method of a nicotinamide microcapsule. Through this method, a nicotinamide microcapsule can be prepared. The nicotinamide microcapsule has good embedding effect, high embedding rate, good embedding ability for nicotinamide, can effectively protect the activity of nicotinamide, improve the absorption efficiency of nicotinamide, and enable nicotinamide to be converted into more NAD + ,thus generating more ATP and enhancing the bioavailability of nicotinamide.

[0006] The present invention also provides a nicotinamide microcapsule, which is prepared by the above preparation method. Therefore, the nicotinamide microcapsule has good embedding effect, high embedding rate, good embedding ability for nicotinamide, can effectively protect the activity of nicotinamide, improve the absorption efficiency of nicotinamide, and enable nicotinamide to be converted into more NAD + ,thus generating more ATP and enhancing the bioavailability of nicotinamide.

[0007] The present invention also provides the use of the above-mentioned nicotinamide microcapsules or nicotinamide microcapsules prepared by the preparation method of the above-mentioned nicotinamide microcapsules in the preparation of a preparation for promoting cell energy metabolism, and the preparation for promoting cell energy metabolism is used to increase the ATP content. Through research by the inventor, it is shown that using the nicotinamide microcapsules of the present invention can produce more ATP.

[0008] The first aspect of the present invention provides a preparation method of nicotinamide microcapsules, comprising the following steps:

[0009] Mix gelatin with water and then carry out heat treatment to obtain a gelatin solution;

[0010] At a constant temperature of 38-45 °C, sequentially dissolve L-α-glycerylphosphorylcholine, sodium alginate, isomaltooligosaccharide, nicotinamide and vitamin C in the gelatin solution to obtain a mixed solution;

[0011] Adjust the pH value of the mixed solution and then carry out continuous stirring to obtain the nicotinamide microcapsules.

[0012] For the preparation method of the nicotinamide microcapsules as described above, by mass fraction, the gelatin is 3-6 parts, the L-α-glycerylphosphorylcholine is 2-5 parts, the sodium alginate is 2-5 parts, the isomaltooligosaccharide is 18-25 parts, the nicotinamide is 5-15 parts, and the vitamin C is 0.5-3 parts.

[0013] For the preparation method of the nicotinamide microcapsules as described above, in the gelatin solution, the mass ratio of gelatin to water is 1:(15-20).

[0014] For the preparation method of the nicotinamide microcapsules as described above, the nicotinamide microcapsules are in a spindle-shaped structure.

[0015] For the preparation method of the nicotinamide microcapsules as described above, the particle size of the gelatin ≤ 300 mesh, the particle size of the L-α-glycerylphosphorylcholine ≤ 300 mesh, the particle size of the sodium alginate ≤ 300 mesh, the particle size of the isomaltooligosaccharide ≤ 300 mesh, the particle size of the nicotinamide ≤ 300 mesh, and the particle size of the vitamin C ≤ 300 mesh.

[0016] For the preparation method of the nicotinamide microcapsules as described above, adjusting the pH value of the mixed solution includes:

[0017] Adjust the pH value of the mixed solution to 3.8-4.2 with a pH regulator;

[0018] The pH regulator is citric acid.

[0019] For the preparation method of the nicotinamide microcapsules as described above, the temperature of the heat treatment is 35-40 °C and the time is 3-5 min.

[0020] The method for preparing the nicotinamide microcapsules as described above, wherein the rotation speed of the continuous stirring is 200 - 500 r / min and the time is 20 - 30 min.

[0021] The second aspect of the present invention provides a kind of nicotinamide microcapsules, which are prepared by the method for preparing the nicotinamide microcapsules as described above.

[0022] The third aspect of the present invention provides the use of the nicotinamide microcapsules as described above or the nicotinamide microcapsules prepared by the method for preparing the nicotinamide microcapsules as described above in the preparation of a preparation for promoting cell energy metabolism, and the preparation for promoting cell energy metabolism is used to increase the content of ATP.

[0023] The solution of the present invention has at least the following effects:

[0024] The method for preparing the nicotinamide microcapsules provided by the present invention, through the synergistic effect among nicotinamide, gelatin, L-α-glycerylphosphorylcholine and other substances, can prepare nicotinamide microcapsules with good embedding effect and high embedding rate. It has good embedding ability for nicotinamide, can effectively protect the activity of nicotinamide, improve the absorption efficiency of nicotinamide, and enable nicotinamide to be converted into more nicotinamide adenine dinucleotide (NAD + ), thereby generating more ATP and enhancing the bioavailability of nicotinamide; this method does not need to use traditional physical embedding processes such as spray drying and coagulation bath, avoiding the problem of premature release of nicotinamide caused by high temperature or high pressure environment in the traditional method, and has the advantages of simple process and low cost; this method shortens and simplifies the capsule preparation process, provides a fast and efficient method for the preparation of microcapsules, and is suitable for wide promotion and application. Description of the Drawings

[0025] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required to be used in the description of the embodiments or the prior art. Obviously, the following drawings are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can be obtained according to these drawings.

[0026] Figure 1 It is the scanning electron microscope image of the nicotinamide microcapsules in Example 1 of the present invention;

[0027] Figure 2 It is the scanning electron microscope image of the nicotinamide microcapsules in Comparative Example 1 of the present invention;

[0028] Figure 3 It is the scanning electron microscope image of the nicotinamide microcapsules in Comparative Example 2 of the present invention;

[0029] Figure 4SEM image of the nicotinamide microcapsules in Comparative Example 3 of the present invention;

[0030] Figure 5 SEM image of the nicotinamide microcapsules in Comparative Example 4 of the present invention;

[0031] Figure 6 Particle size diagram of the nicotinamide microcapsules in Example 1 of the present invention. Detailed implementation manners

[0032] To make the objectives, technical solutions and advantages of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts fall within the scope of protection of the present invention.

[0033] The raw materials and reagents used in the following embodiments can be obtained from commercial channels without special instructions; the processes used, without special instructions, are conventional processes in the art.

[0034] It should be noted that the descriptions involving "first", "second", "third", "fourth", etc. in the present invention are used to distinguish similar objects, rather than to describe a specific order or sequence, and therefore should not be construed as a limitation to the present invention.

[0035] The first aspect of the present invention provides a method for preparing nicotinamide microcapsules, which includes the following steps:

[0036] Mix gelatin with water and then perform heat treatment to obtain a gelatin solution;

[0037] At a constant temperature of 38 - 45 °C, sequentially dissolve L-α-glycerylphosphorylcholine, sodium alginate, isomaltooligosaccharide, nicotinamide, and vitamin C in the gelatin solution to obtain a mixed solution;

[0038] Adjust the pH value of the mixed solution and then continuously stir to obtain nicotinamide microcapsules.

[0039] The present invention does not particularly limit the specific sources of the above raw materials, which can be purchased from commercial channels.

[0040] The above-mentioned nicotinamide (NAM) is a B vitamin, which is a precursor of NAD+ (nicotinamide adenine dinucleotide), and has functions such as enhancing the skin barrier function, inhibiting melanin production, and reducing inflammatory responses; nicotinamide can be converted into NAD + , NAD + The increase in the level of NAD can effectively promote ATP production, thereby enhancing cell energy metabolism.

[0041] In the present invention, the above-mentioned nicotinamide microcapsules are in a liquid state.

[0042] First, gelatin is heat-treated to form a gelatin solution. The heat treatment is to convert gelatin into a liquid state to form a uniform gelatin solution for subsequent mixing with other components, providing a basis for the formation of subsequent nicotinamide microcapsules. Under the constant temperature condition of 38 - 45 °C, first, L-α-glycerylphosphorylcholine is dissolved in the gelatin solution to obtain a first mixed solution, then sodium alginate is dissolved in the first mixed solution to obtain a second mixed solution, then isomaltooligosaccharide is dissolved in the second mixed solution to obtain a third mixed solution, then nicotinamide is dissolved in the third mixed solution to obtain a fourth mixed solution, and finally, vitamin C is dissolved in the fourth mixed solution to obtain a mixed solution. The pH value of the mixed solution is adjusted, and after continuous stirring, nicotinamide microcapsules are obtained. The purpose of continuous stirring after adjusting the pH value of the mixed solution is to make the wall materials (gelatin, sodium alginate, isomaltooligosaccharide, and vitamin C) fully stretch their molecular network structures under the action of electric charges. Then, under the action of continuous stirring (providing shear mechanical force), the core materials (nicotinamide and L-α-glycerylphosphorylcholine) are synergistically embedded in the stretched molecular network structures of the wall materials, enabling the stretched molecular network structures to fully capture the core materials (nicotinamide and L-α-glycerylphosphorylcholine), thereby forming nicotinamide microcapsules.

[0043] Through the above preparation method, the present invention can prepare nicotinamide microcapsules with good embedding effect and high embedding rate. It has good embedding ability for nicotinamide, can effectively protect the activity of nicotinamide, improve the absorption efficiency of nicotinamide, and enable nicotinamide to be converted into more NAD + , thereby generating more ATP and enhancing the bioavailability of nicotinamide. This method does not require traditional physical embedding processes such as spray drying and coagulation bath, avoiding the problem of premature release of nicotinamide caused by high-temperature or high-pressure environments in traditional methods. It has the advantages of simple process and low cost. This method shortens and simplifies the capsule preparation process, providing a fast and efficient method for the preparation of microcapsules, which is suitable for wide promotion and application.

[0044] In a specific embodiment, by mass, 3 - 6 parts of gelatin, 2 - 5 parts of L-α-glycerylphosphorylcholine, 2 - 5 parts of sodium alginate, 18 - 25 parts of isomaltooligosaccharide, 5 - 15 parts of nicotinamide, and 0.5 - 3 parts of vitamin C.

[0045] When the mass parameters of gelatin, L-α-glycerylphosphorylcholine, sodium alginate, isomaltooligosaccharide, nicotinamide, and vitamin C are within the above ranges respectively, the components can effectively play a synergistic role, thereby preparing nicotinamide microcapsules with good embedding effect and high embedding rate.

[0046] Further, by mass parts, 5 parts of gelatin, 3 parts of L-α-glycerylphosphorylcholine, 3 parts of sodium alginate, 20 parts of isomaltooligosaccharide, 10 parts of niacinamide, and 1.5 parts of vitamin C.

[0047] When the mass parameters of gelatin, L-α-glycerylphosphorylcholine, sodium alginate, isomaltooligosaccharide, niacinamide, and vitamin C are each within the above values, it is possible to make the components play a more effective synergistic role, thereby preparing nicotinamide microcapsules with better embedding effect and higher embedding rate.

[0048] In a specific embodiment, in the above gelatin solution, the mass ratio of gelatin to water is 1:(15 - 20), for example, the mass ratio of gelatin to water is 1:15, 1:16, 1:17, 1:18, 1:19, 1:20, etc.

[0049] When the mass ratio of gelatin to water in the gelatin solution is within the above range, it is beneficial to the uniform mixing of the gelatin solution with components such as L-α-glycerylphosphorylcholine, sodium alginate, isomaltooligosaccharide, niacinamide, and vitamin C, and local gelation is not likely to occur under the condition of the pH value, which is beneficial to preparing nicotinamide microcapsules with good embedding effect and high embedding rate subsequently.

[0050] In a specific embodiment, the above nicotinamide microcapsules have a spindle-shaped structure.

[0051] In the present invention, the nicotinamide microcapsules have a spindle-shaped structure, and the spindle-shaped structure has a large specific surface area, which can more effectively protect nicotinamide.

[0052] In a specific embodiment, the particle size of the above gelatin ≤ 300 mesh, the particle size of the above L-α-glycerylphosphorylcholine ≤ 300 mesh, the particle size of the above sodium alginate ≤ 300 mesh, the particle size of the above isomaltooligosaccharide ≤ 300 mesh, the particle size of the above niacinamide ≤ 300 mesh, and the particle size of the above vitamin C ≤ 300 mesh.

[0053] In the present invention, the particle size ≤ 300 mesh means a positive number with a particle size ≤ 300 mesh.

[0054] When the particle sizes of gelatin, L-α-glycerylphosphorylcholine, sodium alginate, isomaltooligosaccharide, niacinamide, and vitamin C are all ≤ 300 mesh, the particle sizes of the components can be controlled to be uniform, which is convenient for the components to be mixed evenly and come into full contact, and prevent the phenomenon of caking or aggregation. Among them, gelatin, sodium alginate, isomaltooligosaccharide, and vitamin C are used as wall materials, and niacinamide and L-α-glycerylphosphorylcholine are used as core materials.

[0055] In a specific embodiment, adjusting the pH value of the mixed solution includes: adjusting the pH value of the mixed solution to 3.8 - 4.2 with a pH regulator.

[0056] The pH value of the mixed solution is adjusted to 3.8 - 4.2 by the pH regulator of the present invention, which can cause the molecular network structure of the wall material (gelatin, sodium alginate, isomaltooligosaccharide, and vitamin C) to stretch to the greatest extent under the action of charge, facilitating the capture (embedding) of more core materials (niacinamide and L-α-glycerylphosphorylcholine), thereby preparing nicotinamide microcapsules with good embedding effect and high embedding rate.

[0057] In a specific embodiment, the above pH regulator is citric acid.

[0058] When the pH regulator is citric acid, it can cause the molecular network structure of the wall material (gelatin, sodium alginate, isomaltooligosaccharide, and vitamin C) to stretch to the greatest extent under the action of charge, facilitating an increase in the contact surface with the core materials (niacinamide and L-α-glycerylphosphorylcholine), capturing (embedding) more core materials (niacinamide and L-α-glycerylphosphorylcholine), thereby preparing nicotinamide microcapsules with good embedding effect and high embedding rate.

[0059] In a specific embodiment, the temperature of the above heat treatment is 35 - 40 °C and the time is 3 - 5 min.

[0060] When the parameters of the temperature and time of the heat treatment are within the above ranges respectively, it can completely dissolve the gelatin, avoiding local overheating or insufficient dissolution.

[0061] Exemplarily, the temperature of the heat treatment can be any one of 35 °C, 36 °C, 37 °C, 38 °C, 39 °C, 40 °C or the range composed of any two of them;

[0062] The time can be any one of 3 min, 4 min, 5 min or the range composed of any two of them.

[0063] In a specific embodiment, the rotation speed of the above continuous stirring is 200 - 500 r / min and the time is 20 - 30 min.

[0064] Exemplarily, the rotation speed of the continuous stirring is any one of 200 r / min, 250 r / min, 300 r / min, 350 r / min, 400 r / min, 450 r / min, 500 r / min or the range composed of any two of them;

[0065] The time can be any one of 20 min, 21 min, 22 min, 23 min, 24 min, 25 min, 26 min, 27 min, 28 min, 29 min, 30 min or the range composed of any two of them.

[0066] When the parameters of the rotation speed and time of continuous stirring are within the above ranges respectively, the nicotinamide and L-α-glycerylphosphorylcholine in the mixed solution can be embedded in the stretched molecular network structure of the wall material under the action of shear mechanical force, promoting the formation of nicotinamide microcapsules. If the continuous stirring time is too short (less than 20 min), the wall material will fail to effectively encapsulate the core material; if the continuous stirring time is too long (more than 30 min), the microcapsule structure will be damaged.

[0067] The second aspect of the present invention provides a nicotinamide microcapsule, which is prepared by the preparation method of the above nicotinamide microcapsule. Therefore, this nicotinamide microcapsule has good embedding effect, high embedding rate, good embedding ability for nicotinamide, can effectively protect the activity of nicotinamide, improve the absorption efficiency of nicotinamide, and enable nicotinamide to be converted into more NAD + , thereby generating more ATP and enhancing the bioavailability of nicotinamide.

[0068] The third aspect of the present invention provides the use of the above nicotinamide microcapsule or the nicotinamide microcapsule prepared by the preparation method of the above nicotinamide microcapsule in the preparation of a preparation for promoting cell energy metabolism, and this preparation for promoting cell energy metabolism is used to increase the content of ATP.

[0069] Hereinafter, the present invention will be further introduced through specific examples.

[0070] Example 1

[0071] This example provides a preparation method of nicotinamide microcapsules, which includes the following steps:

[0072] (1) Respectively screen gelatin, L-α-glycerylphosphorylcholine, sodium alginate, isomaltooligosaccharide, nicotinamide, and vitamin C with a 300-mesh sieve to obtain gelatin with a particle size ≤ 300 mesh, L-α-glycerylphosphorylcholine with a particle size ≤ 300 mesh, sodium alginate with a particle size ≤ 300 mesh, isomaltooligosaccharide with a particle size ≤ 300 mesh, nicotinamide with a particle size ≤ 300 mesh, and vitamin C with a particle size ≤ 300 mesh;

[0073] (2) By mass, mix 5 parts of gelatin with a particle size ≤ 300 mesh and 90 parts of water, and heat-treat at 40 °C for 5 min to prepare a gelatin solution;

[0074] (3) Under the condition of constant temperature at 40 °C, sequentially dissolve 3 parts of L-α-glycerylphosphorylcholine with a particle size ≤ 300 mesh, 3 parts of sodium alginate with a particle size ≤ 300 mesh, 20 parts of isomaltooligosaccharide with a particle size ≤ 300 mesh, 10 parts of nicotinamide with a particle size ≤ 300 mesh, and 1.5 parts of vitamin C with a particle size ≤ 300 mesh in the gelatin solution to obtain a mixed solution;

[0075] (4) After adjusting the pH value of the mixed solution to 4 with citric acid, continuously stir at 300 r / min for 20 min to obtain nicotinamide microcapsules.

[0076] Example 2

[0077] The preparation method of nicotinamide microcapsules provided in this example is basically the same as that in Example 1, except that:

[0078] (4) After adjusting the pH value of the mixed solution to 3.8 with citric acid.

[0079] Example 3

[0080] The preparation method of nicotinamide microcapsules provided in this example is basically the same as that in Example 1, except that:

[0081] (4) After adjusting the pH value of the mixed solution to 4.2 with citric acid.

[0082] Comparative Example 1

[0083] The preparation method of nicotinamide microcapsules provided in this comparative example is basically the same as that in Example 1, except that:

[0084] Replace gelatin with soy protein isolate.

[0085] Comparative Example 2 (without adding gelatin)

[0086] The preparation method of nicotinamide microcapsules provided in this comparative example is basically the same as that in Example 1, except that:

[0087] (1) Respectively sieve L-α-glycerylphosphorylcholine, sodium alginate, isomaltooligosaccharide, nicotinamide, and vitamin C through a 300-mesh sieve to obtain L-α-glycerylphosphorylcholine with a particle size ≤ 300 mesh, sodium alginate with a particle size ≤ 300 mesh, isomaltooligosaccharide with a particle size ≤ 300 mesh, nicotinamide with a particle size ≤ 300 mesh, and vitamin C with a particle size ≤ 300 mesh;

[0088] (2) By mass, mix 3 parts of sodium alginate with a particle size ≤ 300 mesh and 90 parts of water, and heat-treat at 40 °C for 5 min to prepare a sodium alginate solution;

[0089] (3) Under the condition of constant temperature at 40 °C, successively dissolve 3 parts of L-α-glycerylphosphorylcholine with a particle size ≤ 300 mesh, 20 parts of isomaltooligosaccharide with a particle size ≤ 300 mesh, 10 parts of nicotinamide with a particle size ≤ 300 mesh, and 1.5 parts of vitamin C with a particle size ≤ 300 mesh in the sodium alginate solution to obtain a mixed solution;

[0090] (4) After adjusting the pH value of the mixed solution to 4 with citric acid, continuously stir at 300 r / min for 20 min to obtain nicotinamide microcapsules.

[0091] Comparative Example 3 (without adding sodium alginate)

[0092] The preparation method of the nicotinamide microcapsules provided in this comparative example is basically the same as that of Example 1, except that:

[0093] (3) Under the condition of constant temperature at 40 °C, 3 parts of L-α-glycerylphosphorylcholine with a particle size ≤ 300 mesh, 20 parts of isomaltooligosaccharide with a particle size ≤ 300 mesh, 10 parts of nicotinamide with a particle size ≤ 300 mesh, and 1.5 parts of vitamin C with a particle size ≤ 300 mesh were successively dissolved in the gelatin solution to obtain a mixed solution.

[0094] Comparative Example 4 (without adding L-α-glycerylphosphorylcholine)

[0095] The preparation method of the nicotinamide microcapsules provided in this comparative example is basically the same as that of Example 1, except that:

[0096] (3) Under the condition of constant temperature at 40 °C, 3 parts of sodium alginate with a particle size ≤ 300 mesh, 20 parts of isomaltooligosaccharide with a particle size ≤ 300 mesh, 10 parts of nicotinamide with a particle size ≤ 300 mesh, and 1.5 parts of vitamin C with a particle size ≤ 300 mesh were successively dissolved in the gelatin solution to obtain a mixed solution.

[0097] Result description

[0098] 1. Scanning electron microscopy test

[0099] The nicotinamide microcapsules in Example 1 and Comparative Examples 1-4 of the present invention were respectively subjected to scanning electron microscopy test. Figure 1 This is the scanning electron micrograph of the nicotinamide microcapsules in Example 1 of the present invention. Figure 2 This is the scanning electron micrograph of the nicotinamide microcapsules in Comparative Example 1 of the present invention. Figure 3 This is the scanning electron micrograph of the nicotinamide microcapsules in Comparative Example 2 of the present invention. Figure 4 This is the scanning electron micrograph of the nicotinamide microcapsules in Comparative Example 3 of the present invention. Figure 5 This is the scanning electron micrograph of the nicotinamide microcapsules in Comparative Example 4 of the present invention.

[0100] It can be seen from Figure 1 that the nicotinamide microcapsules in Example 1 of the present invention have an obvious microcapsule structure, which is a spindle-shaped structure. The spindle-shaped structure has a large specific surface area and can more effectively protect nicotinamide.

[0101] It can be seen from Figure 2 that there is no obvious microcapsule structure in the nicotinamide microcapsules in Comparative Example 1 of the present invention.

[0102] It can be seen from Figure 3 that there is no obvious microcapsule structure in the nicotinamide microcapsules in Comparative Example 2 of the present invention.

[0103] As can be seen from Figure 4 Figure 3, the nicotinamide microcapsules in Comparative Example 3 of the present invention have an obvious microcapsule structure, which is an irregular flat structure and has an unstable structure.

[0104] As can be seen from Figure 5 Figure 4, the nicotinamide microcapsules in Comparative Example 4 of the present invention have an obvious microcapsule structure, which is an irregular circular structure.

[0105] 2. Particle size test

[0106] After confirming the existence of the microcapsule structure of the nicotinamide microcapsules in Example 1 of the present invention, the particle size of the nicotinamide microcapsules in Example 1 was tested; Figure 6 Figure 5 is the particle size diagram of the nicotinamide microcapsules in Example 1 of the present invention.

[0107] As can be seen from Figure 6 Figure 5, the particle size of the nicotinamide microcapsules in Example 1 of the present invention is about 1000 nm, showing good uniformity.

[0108] 3. Encapsulation efficiency test

[0109] The encapsulation efficiency of nicotinamide in the nicotinamide microcapsules of Examples 1-3 and Comparative Examples 1-4 of the present invention was tested by high performance liquid chromatography (HPLC) method, and the test results are shown in Table 1:

[0110] Table 1 Test results

[0111]

[0112]

[0113] As can be seen from Table 1, by comparing Example 1 with Comparative Examples 1-4, it is found that the nicotinamide microcapsules in Example 1 of the present invention have the best encapsulation effect, a high encapsulation efficiency, and good encapsulation ability for nicotinamide.

[0114] 4. Determination of intracellular ATP content

[0115] Using the in vitro simulated human gastrointestinal digestion technology, the ATP content of the nicotinamide microcapsules in Example 1 and Comparative Examples 1-4 of the present invention was measured respectively. The specific method is as follows:

[0116] Undigested sample: Weigh 100 mg of the sample, dissolve it in 50 mL of 0.9% NaCl, and add 0.5 mL of 1 mol / L HCl to obtain an undigested solution.

[0117] Digested sample: The in vitro simulated digestion process lasted for 4 h and was divided into two stages: ① The first stage was gastric digestion: 100 mg of the sample was weighed, dissolved in 50 mL of 0.9% NaCl, 0.5 mL of 1 mol / L HCl was added, and then 150 mg of pepsin was added to obtain a mixed solution. The mixed solution was placed in a constant temperature shaking incubator and incubated at 37 °C in the dark with shaking for 2 h to obtain the solution after gastric digestion; ② The second stage was intestinal digestion: 10 mL of 0.5 mol / L NaHCO3 solution was added dropwise to the solution after gastric digestion, and then 18 mL of the mixture (containing 2 mg / mL of trypsin solution and 12 mg / mL of cholate, V:V = 12:6) was added, and it was incubated at 37 °C in the dark with shaking for 2 h to obtain the solution after intestinal digestion.

[0118] The nicotinamide microcapsules in Example 1 and Comparative Examples 1-4 of the present invention were used as samples respectively, and the undigested solutions, solutions after gastric digestion, and solutions after intestinal digestion of Example 1 and each comparative example were obtained respectively according to the above method.

[0119] Samples were taken from the undigested solutions, solutions after gastric digestion, and solutions after intestinal digestion of Example 1 and each comparative example respectively, and then 1 mL of the extraction solution (the extraction solution in the ATP content assay kit (Shanghai Fuda Testing Technology Group Co., Ltd.)) was added, and ice bath homogenization was carried out. Centrifugation was carried out at 12000 rpm and 4 °C for 10 min, and the supernatant was taken. The ATP content was measured according to the instruction manual of the ATP content kit (Shanghai Fuda Testing Technology Group Co., Ltd.). The ATP content measurement results are shown in Table 2.

[0120] Table 2 ATP content measurement results

[0121] Item ATP content (μmol / mL) Undigested solution (Example 1) 0.730 Solution after gastric digestion (Example 1) 0.927 Solution after intestinal digestion (Example 1) 0.610 Undigested solution (Comparative Example 1) 0.683 Solution after gastric digestion (Comparative Example 1) 0.772 Solution after intestinal digestion (Comparative Example 1) 0.516 Undigested solution (Comparative Example 2) 0.604 Solution after gastric digestion (Comparative Example 2) 0.781 Solution after intestinal digestion (Comparative Example 2) 0.508 Undigested solution (Comparative Example 3) 0.682 Solution after gastric digestion (Comparative Example 3) 0.789 Solution after intestinal digestion (Comparative Example 3) 0.598 Undigested solution (Comparative Example 4) 0.634 Solution after gastric digestion (Comparative Example 4) 0.788 Solution after intestinal digestion (Comparative Example 4) 0.523

[0122] During the gastrointestinal digestion and absorption process, the absorption of many nutrients is carried out by active transport, which requires ATP to provide energy. A high ATP content means that the cells have a strong active transport ability, which can transport nutrients in the intestine against the concentration gradient into the cells, thereby improving the absorption efficiency of nutrients. As can be seen from Table 2, by comparing Example 1 with Comparative Examples 1-4, it was found that the ATP contents of the nicotinamide microcapsules in Example 1 before digestion, after gastric digestion, and after gastric and intestinal digestion were all higher than those of Comparative Examples 1-4, indicating that the nicotinamide microcapsules prepared by the method of Example of the present invention can effectively improve the absorption efficiency of nicotinamide, enabling nicotinamide to be converted into more NAD + and thus generating more ATP and enhancing the bioavailability of nicotinamide.

[0123] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that they can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements on some or all of the technical features; and these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A method for preparing nicotinamide microcapsules, characterized in that, It includes the following steps: Mix gelatin with water and then conduct heat treatment to obtain a gelatin solution; At a constant temperature of 38 - 45 °C, sequentially dissolve L-α-glycerylphosphorylcholine, sodium alginate, isomaltooligosaccharide, niacinamide, and vitamin C in the gelatin solution to obtain a mixed solution; Adjust the pH value of the mixed solution and then conduct continuous stirring to obtain the niacinamide microcapsules.

2. The preparation method of the nicotinamide microcapsules according to claim 1, wherein, By mass fraction, 3 - 6 parts of gelatin, 2 - 5 parts of L-α-glycerylphosphorylcholine, 2 - 5 parts of sodium alginate, 18 - 25 parts of isomaltooligosaccharide, 5 - 15 parts of niacinamide, and 0.5 - 3 parts of vitamin C.

3. The preparation method of the nicotinamide microcapsules according to claim 2, characterized in that, In the gelatin solution, the mass ratio of gelatin to water is 1:(15 - 20).

4. The preparation method of the nicotinamide microcapsules according to claim 1, characterized in that, The niacinamide microcapsules are in a spindle-shaped structure.

5. The preparation method of the nicotinamide microcapsules according to claim 1, characterized in that, The particle size of the gelatin ≤ 300 mesh, the particle size of the L-α-glycerylphosphorylcholine ≤ 300 mesh, the particle size of the sodium alginate ≤ 300 mesh, the particle size of the isomaltooligosaccharide ≤ 300 mesh, the particle size of the niacinamide ≤ 300 mesh, and the particle size of the vitamin C ≤ 300 mesh.

6. The preparation method of the nicotinamide microcapsules according to claim 1, wherein Adjusting the pH value of the mixed solution includes: Use a pH regulator to adjust the pH value of the mixed solution to 3.8 - 4.2; The pH regulator is citric acid.

7. The preparation method of the nicotinamide microcapsules according to claim 1, wherein, The temperature of the heat treatment is 35 - 40 °C and the time is 3 - 5 min.

8. The preparation method of the nicotinamide microcapsules according to claim 1, characterized in that, The rotation speed of the continuous stirring is 200 - 500 r / min and the time is 20 - 30 min.

9. A nicotinamide microcapsule, characterized in that, It is prepared by the preparation method of the niacinamide microcapsules according to any one of claims 1 - 8.

10. Use of the nicotinamide microcapsule according to claim 9 or the nicotinamide microcapsule prepared by the preparation method of the nicotinamide microcapsule according to any one of claims 1-8 in the preparation of a preparation for promoting cell energy metabolism, characterized in that, The preparation for promoting cell energy metabolism is used to increase the ATP content.

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