Organic fertilizer based on biomass fermentation and preparation method and application thereof

Abstract

The application discloses a kind of organic fertilizer based on biomass fermentation and its preparation method and application, belong to biological organic fertilizer technical field.The organic fertilizer based on biomass fermentation of the application includes the following raw materials: chicken manure 40-50 parts, rapeseed cake 20-30 parts, seaweed powder 15-20 parts, corn straw 10-15 parts, EM bacterial liquid 10-15 parts, compound microbial inoculant 6-10 parts, Rhizoma Cyperi 8-16 parts, mulberry branch 4-8 parts, water hyacinth 12-18 parts, mushroom residue 5-8 parts, wherein the compound microbial inoculant is composed of jelly-like bacillus, sand on flavobacterium, rhizosphere oligotrophic single-cell bacteria, which can quickly decompose organic matter, accelerate the production of small molecules, and be directly absorbed by plants and produce a variety of active substances during fermentation.The organic fertilizer of the application has a synergistic effect of multiple substances, significantly promotes photosynthesis of plants, improves nutrient production and transportation, effectively promotes growth of Chinese cabbage and improves quality of Chinese cabbage.

Description

Technical Field

[0001] This invention belongs to the field of bio-organic fertilizer technology, specifically relating to an organic fertilizer based on biomass fermentation, its preparation method, and its application. Background Technology

[0002] Bok choy (Brassica campestris L.ssp.chinensis Makino) is an annual or biennial herbaceous plant belonging to the Brassicaceae family and the Brassica genus. It prefers cool climates, but its seedlings are adaptable to a wide range of temperatures, making it an important leafy green vegetable available year-round. Bok choy is rich in nutrients, containing protein, fat, dietary fiber, and various vitamins and trace elements, with a relatively high calcium content.

[0003] Agricultural waste mainly includes crop straw and livestock manure. Due to limitations in economic conditions, utilization methods, and other related factors, most of this waste is discarded indiscriminately, wasting resources and causing significant environmental pollution. Most agricultural waste contains abundant organic matter and relatively high levels of nutrients such as nitrogen, phosphorus, and potassium required by crops, making it an excellent source for producing organic fertilizer. Organic fertilizer prepared using proper composting techniques can enhance microbial activity, increase nutrient availability, improve soil fertility and enzyme activity, and improve the quality of agricultural products while eliminating toxic and harmful substances from the waste and increasing its utilization rate.

[0004] Chinese patent CN102351596B discloses an organic compound fertilizer for bok choy. This fertilizer mixes various micronutrients required by plants with inorganic and organic fertilizers, and adds additives to improve plant absorption and utilization rates and enhance slow-release properties. It also incorporates effective microbial flora and, through appropriate fermentation and processing, produces a special granular compound fertilizer to meet the growth needs of bok choy. However, this invention does not improve the quality of bok choy and no longer meets current consumer demands for organic vegetables. Therefore, developing an organic fertilizer based on biomass fermentation to effectively promote bok choy growth and improve its quality is of great significance for bok choy cultivation. Summary of the Invention

[0005] The purpose of this invention is to provide an organic fertilizer based on biomass fermentation that effectively promotes the growth of bok choy and improves its quality.

[0006] To achieve the above-mentioned technical objectives, the technical solution adopted by the present invention is as follows:

[0007] An organic fertilizer based on biomass fermentation comprises the following raw materials in parts by weight: 40-50 parts chicken manure, 20-30 parts rapeseed cake, 15-20 parts seaweed powder, 10-15 parts corn stalks, 10-15 parts EM bacterial solution, 6-10 parts compound microbial agent, 8-16 parts Cyperus rotundus, 4-8 parts mulberry branches, 12-18 parts water hyacinth, and 5-8 parts mushroom residue.

[0008] Furthermore, the compound microbial agent consists of Bacillus jellyiformis, Flavobacterium sandae, and Oligotrophic radiculi in a mass ratio of 1:1:1.

[0009] Furthermore, the gelatinous Bacillus was purchased from the China Center for Type Culture Collection (CCTCC) with accession number CCTCC AB 2019184 and original accession date of June 21, 2019; the Flavobacterium sarsinis was purchased from the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC 1.12366 and original accession date of October 15, 2012; and the Stenotrophomonas radiata was purchased from the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC 1.15515 and original accession date of January 25, 2016.

[0010] A method for preparing organic fertilizer based on biomass fermentation includes the following steps:

[0011] (1) Preparation of compound microbial inoculants:

[0012] Bacillus jellyoides, Flavobacterium sandae, and Oligotrophic rhizogenes were cultured in seed culture media at 28℃, 30℃, and 30℃ for 48 h, respectively. They were then inoculated into LB medium at a 1% inoculum and cultured until the bacterial concentration reached OD500. 600 =3.0 Three bacterial solutions were obtained. The three bacterial solutions were mixed evenly in a mass ratio of 1:1:1 and freeze-dried into freeze-dried powder to obtain a compound microbial agent.

[0013] (2) Preparation of fermented products of Cyperus rotundus, mulberry twigs, and water hyacinth:

[0014] After drying Cyperus rotundus, water hyacinth, and mulberry branches, the mixture is pulverized to obtain a mixed powder, which is then passed through an 80-mesh sieve and microwaved for 5 minutes. A complex hydrolytic enzyme solution containing cellulase, xylanase, and β-glucosidase is added, and the mixture is enzymatically hydrolyzed at 45-55℃ for 6-8 hours. After high-temperature inactivation, the mixture is cooled to room temperature and set aside. A complex microbial agent is then added to the mixture, and fermentation is carried out at 30℃ for 24-48 hours.

[0015] (3) Fermentation and composting:

[0016] Chicken manure, rapeseed cake, seaweed powder, corn stalks and mushroom residue are mixed and dried in a ventilated manner until the moisture content is <5%. They are then crushed and passed through an 80-mesh sieve, stirred and mixed evenly, and added to a fermentation tank. The moisture content is adjusted to 40-50%. While stirring the fermentation substrate, EM bacterial solution is sprayed in so that the EM bacterial solution is evenly sprayed into the fermentation substrate. The fermentation is carried out in a ventilated manner for 48 hours. Then, the fermented products of Cyperus rotundus, mulberry branches and water hyacinth prepared in step (2) are added and stirred and mixed evenly. The fermentation tank is sealed with plastic film and the top layer is compacted with soil. During this period, the pile is turned over every 5 days until the fermentation is completed. The plastic film is then opened to obtain the fermented organic matter.

[0017] (4) Granulation:

[0018] The fermented organic matter obtained in step (3) is dried in a ventilator until the moisture content is ≤20%. It is then granulated using a disc granulator to control the particle size to 3-5 mm. The granules are then dried in a dryer until the moisture content is ≤8%. The mixture is then allowed to cool naturally to room temperature to obtain the final product, organic fertilizer based on biomass fermentation.

[0019] Furthermore, in step (2), the mass-to-volume ratio of the mixed powder of Cyperus rotundus, water hyacinth, and mulberry twig to the compound hydrolytic enzyme is 1 kg: (1.5-2.0) L.

[0020] Furthermore, the complex hydrolase contains 16-18 g / L of cellulase, 8-12 g / L of xylanase, and 14-22 g / L of β-glucosidase.

[0021] This invention also provides the application of the aforementioned biomass fermentation-based organic fertilizer in promoting vegetable growth and improving quality.

[0022] Furthermore, the vegetable in question is bok choy.

[0023] All raw materials used in this invention are commercially available.

[0024] Beneficial effects

[0025] This invention is based on organic fertilizer produced through biomass fermentation, with added ingredients such as Cyperus rotundus, water hyacinth, and mulberry branches. First, the cell wall structure of Cyperus rotundus, water hyacinth, and mulberry branches is enzymatically broken down to effectively release nutrients. Then, a compound microbial agent composed of Bacillus spp., Flavobacterium sarcodactylis, and Oligotrophic bacilli is added for fermentation. These microorganisms rapidly decompose organic matter, accelerating the production of small molecules that are easily absorbed by vegetables. Furthermore, the fermentation process generates various active substances that effectively promote the growth of bok choy.

[0026] The organic fertilizer of this invention contains a variety of substances that work synergistically to significantly promote photosynthesis in plants, improve the production and transport of nutrients, effectively promote the growth of bok choy and improve its quality.

[0027] This invention relates to an organic fertilizer based on biomass fermentation that utilizes a large amount of agricultural waste, which is widely available, low in cost, simple to prepare, convenient to use, and easy to promote. Detailed Implementation

[0028] The technical solution of the present invention will be further described below with reference to specific embodiments, but it is not limited thereto.

[0029] Example 1

[0030] An organic fertilizer based on biomass fermentation comprises the following raw materials in parts by weight: 40 parts chicken manure, 20 parts rapeseed cake, 15 parts seaweed powder, 10 parts corn stalks, 10 parts EM bacterial solution, 6 parts compound microbial agent, 8 parts Cyperus rotundus, 4 parts mulberry branches, 12 parts water hyacinth, and 5 parts mushroom residue.

[0031] The compound microbial agent consists of Bacillus jellyiformis, Flavobacterium sandae, and Oligotrophic bacillus radiata in a mass ratio of 1:1:1.

[0032] The preservation number of the gelatinous Bacillus is CCTCC AB 2019184; the preservation number of the Flavobacterium sarcodactylis is CGMCC 1.12366; and the preservation number of the Oligotrophomonas radiata is CGMCC 1.15515.

[0033] A method for preparing organic fertilizer based on biomass fermentation includes the following steps:

[0034] (1) Preparation of compound microbial inoculants:

[0035] Bacillus jellyoides, Flavobacterium sandae, and Oligotrophic rhizogenes were cultured in seed culture media at 28℃, 30℃, and 30℃ for 48 h, respectively. They were then inoculated into LB medium at a 1% inoculum and cultured until the bacterial concentration reached OD500. 600 =3.0 Three bacterial solutions were obtained. The three bacterial solutions were mixed evenly in a mass ratio of 1:1:1 and freeze-dried into freeze-dried powder to obtain a compound microbial agent.

[0036] (2) Preparation of fermented products of Cyperus rotundus, mulberry twigs, and water hyacinth:

[0037] After drying Cyperus rotundus, water hyacinth, and mulberry branches, the mixture is pulverized to obtain a mixed powder, which is then passed through an 80-mesh sieve and microwaved for 5 minutes. A complex hydrolytic enzyme solution containing cellulase, xylanase, and β-glucosidase is added, and the mixture is enzymatically hydrolyzed at 45-55℃ for 6-8 hours. After high-temperature inactivation, the mixture is cooled to room temperature and set aside. A complex microbial agent is then added to the mixture, and fermentation is carried out at 30℃ for 24-48 hours.

[0038] (3) Fermentation and composting:

[0039] Chicken manure, rapeseed cake, seaweed powder, corn stalks and mushroom residue are mixed and dried in a ventilated manner until the moisture content is <5%. They are then crushed and passed through an 80-mesh sieve, stirred and mixed evenly, and added to a fermentation tank. The moisture content is adjusted to 40-50%. While stirring the fermentation substrate, EM bacterial solution is sprayed in so that the EM bacterial solution is evenly sprayed into the fermentation substrate. The fermentation is carried out in a ventilated manner for 48 hours. Then, the fermented products of Cyperus rotundus, mulberry branches and water hyacinth prepared in step (2) are added and stirred and mixed evenly. The fermentation tank is sealed with plastic film and the top layer is compacted with soil. During this period, the pile is turned over every 5 days until the fermentation is completed. The plastic film is then opened to obtain the fermented organic matter.

[0040] (4) Granulation:

[0041] The fermented organic matter obtained in step (3) is dried in a ventilator until the moisture content is ≤20%. It is then granulated using a disc granulator to control the particle size to 3-5 mm. The granules are then dried in a dryer until the moisture content is ≤8%. The mixture is then allowed to cool naturally to room temperature to obtain the final product, organic fertilizer based on biomass fermentation.

[0042] In step (2), the mass-to-volume ratio of the mixed powder of Cyperus rotundus, water hyacinth, and mulberry twig to the compound hydrolytic enzyme is 1 kg: 1.5 L.

[0043] The complex hydrolase contains 16 g / L cellulase, 8 g / L xylanase, and 14 g / L β-glucosidase.

[0044] Example 2

[0045] An organic fertilizer based on biomass fermentation comprises the following raw materials in parts by weight: 45 parts chicken manure, 25 parts rapeseed cake, 18 parts seaweed powder, 13 parts corn stalks, 12 parts EM bacterial solution, 8 parts compound microbial agent, 12 parts Cyperus rotundus, 6 parts mulberry branches, 15 parts water hyacinth, and 7 parts mushroom residue.

[0046] The compound microbial agent consists of Bacillus jellyiformis, Flavobacterium sandae, and Oligotrophic bacillus radiata in a mass ratio of 1:1:1.

[0047] The preservation number of the gelatinous Bacillus is CCTCC AB 2019184; the preservation number of the Flavobacterium sarcodactylis is CGMCC 1.12366; and the preservation number of the Oligotrophomonas radiata is CGMCC 1.15515.

[0048] A method for preparing organic fertilizer based on biomass fermentation includes the following steps:

[0049] (1) Preparation of compound microbial inoculants:

[0050] Bacillus jellyoides, Flavobacterium sandae, and Oligotrophic rhizogenes were cultured in seed culture media at 28℃, 30℃, and 30℃ for 48 h, respectively. They were then inoculated into LB medium at a 1% inoculum and cultured until the bacterial concentration reached OD500. 600 =3.0 Three bacterial solutions were obtained. The three bacterial solutions were mixed evenly in a mass ratio of 1:1:1 and freeze-dried into freeze-dried powder to obtain a compound microbial agent.

[0051] (2) Preparation of fermented products of Cyperus rotundus, mulberry twigs, and water hyacinth:

[0052] After drying Cyperus rotundus, water hyacinth, and mulberry branches, the mixture is pulverized to obtain a mixed powder, which is then passed through an 80-mesh sieve and microwaved for 5 minutes. A complex hydrolytic enzyme solution containing cellulase, xylanase, and β-glucosidase is added, and the mixture is enzymatically hydrolyzed at 45-55℃ for 6-8 hours. After high-temperature inactivation, the mixture is cooled to room temperature and set aside. A complex microbial agent is then added to the mixture, and fermentation is carried out at 30℃ for 24-48 hours.

[0053] (3) Fermentation and composting:

[0054] Chicken manure, rapeseed cake, seaweed powder, corn stalks and mushroom residue are mixed and dried in a ventilated manner until the moisture content is <5%. They are then crushed and passed through an 80-mesh sieve, stirred and mixed evenly, and added to a fermentation tank. The moisture content is adjusted to 40-50%. While stirring the fermentation substrate, EM bacterial solution is sprayed in so that the EM bacterial solution is evenly sprayed into the fermentation substrate. The fermentation is carried out in a ventilated manner for 48 hours. Then, the fermented products of Cyperus rotundus, mulberry branches and water hyacinth prepared in step (2) are added and stirred and mixed evenly. The fermentation tank is sealed with plastic film and the top layer is compacted with soil. During this period, the pile is turned over every 5 days until the fermentation is completed. The plastic film is then opened to obtain the fermented organic matter.

[0055] (4) Granulation:

[0056] The fermented organic matter obtained in step (3) is dried in a ventilator until the moisture content is ≤20%. It is then granulated using a disc granulator to control the particle size to 3-5 mm. The granules are then dried in a dryer until the moisture content is ≤8%. The mixture is then allowed to cool naturally to room temperature to obtain the final product, organic fertilizer based on biomass fermentation.

[0057] In step (2), the mass-to-volume ratio of the mixed powder of Cyperus rotundus, water hyacinth, and mulberry twig to the compound hydrolytic enzyme is 1 kg: 2 L.

[0058] The complex hydrolytic enzyme contains 17 g / L cellulase, 10 g / L xylanase, and 17 g / L β-glucosidase.

[0059] Example 3

[0060] An organic fertilizer based on biomass fermentation comprises the following raw materials in parts by weight: 50 parts chicken manure, 30 parts rapeseed cake, 20 parts seaweed powder, 15 parts corn stalks, 15 parts EM bacterial solution, 10 parts compound microbial agent, 16 parts Cyperus rotundus, 8 parts mulberry branches, 18 parts water hyacinth, and 8 parts mushroom residue.

[0061] The compound microbial agent consists of Bacillus jellyiformis, Flavobacterium sandae, and Oligotrophic bacillus radiata in a mass ratio of 1:1:1.

[0062] The preservation number of the gelatinous Bacillus is CCTCC AB 2019184; the preservation number of the Flavobacterium sarcodactylis is CGMCC 1.12366; and the preservation number of the Oligotrophomonas radiata is CGMCC 1.15515.

[0063] A method for preparing organic fertilizer based on biomass fermentation includes the following steps:

[0064] (1) Preparation of compound microbial inoculants:

[0065] Bacillus jellyoides, Flavobacterium sandae, and Oligotrophic rhizogenes were cultured in seed culture media at 28℃, 30℃, and 30℃ for 48 h, respectively. They were then inoculated into LB medium at a 1% inoculum and cultured until the bacterial concentration reached OD500. 600 =3.0 Three bacterial solutions were obtained. The three bacterial solutions were mixed evenly in a mass ratio of 1:1:1 and freeze-dried into freeze-dried powder to obtain a compound microbial agent.

[0066] (2) Preparation of fermented products of Cyperus rotundus, mulberry twigs, and water hyacinth:

[0067] After drying Cyperus rotundus, water hyacinth, and mulberry branches, the mixture is pulverized to obtain a mixed powder, which is then passed through an 80-mesh sieve and microwaved for 5 minutes. A complex hydrolytic enzyme solution containing cellulase, xylanase, and β-glucosidase is added, and the mixture is enzymatically hydrolyzed at 45-55℃ for 6-8 hours. After high-temperature inactivation, the mixture is cooled to room temperature and set aside. A complex microbial agent is then added to the mixture, and fermentation is carried out at 30℃ for 24-48 hours.

[0068] (3) Fermentation and composting:

[0069] Chicken manure, rapeseed cake, seaweed powder, corn stalks and mushroom residue are mixed and dried in a ventilated manner until the moisture content is <5%. They are then crushed and passed through an 80-mesh sieve, stirred and mixed evenly, and added to a fermentation tank. The moisture content is adjusted to 40-50%. While stirring the fermentation substrate, EM bacterial solution is sprayed in so that the EM bacterial solution is evenly sprayed into the fermentation substrate. The fermentation is carried out in a ventilated manner for 48 hours. Then, the fermented products of Cyperus rotundus, mulberry branches and water hyacinth prepared in step (2) are added and stirred and mixed evenly. The fermentation tank is sealed with plastic film and the top layer is compacted with soil. During this period, the pile is turned over every 5 days until the fermentation is completed. The plastic film is then opened to obtain the fermented organic matter.

[0070] (4) Granulation:

[0071] The fermented organic matter obtained in step (3) is dried in a ventilator until the moisture content is ≤20%. It is then granulated using a disc granulator to control the particle size to 3-5 mm. The granules are then dried in a dryer until the moisture content is ≤8%. The mixture is then allowed to cool naturally to room temperature to obtain the final product, organic fertilizer based on biomass fermentation.

[0072] In step (2), the mass-to-volume ratio of the mixed powder of Cyperus rotundus, water hyacinth, and mulberry twig to the compound hydrolytic enzyme is 1 kg: 2.0 L.

[0073] The complex hydrolytic enzyme contains 18 g / L cellulase, 12 g / L xylanase, and 22 g / L β-glucosidase.

[0074] Comparative Example 1

[0075] In this comparative example, compared with Example 3, except that only gelatinous Bacillus was used in the compound microbial agent, all other raw materials and steps were the same as in Example 3.

[0076] Comparative Example 2

[0077] In this comparative example, compared with Example 3, except that only Flavobacterium sandae was used in the compound microbial agent, all other raw materials and steps were the same as in Example 3.

[0078] Comparative Example 3

[0079] In this comparative example, compared with Example 3, except that only Oligotrophomonas rhizophila was used in the compound microbial agent, all other raw materials and steps were the same as in Example 3.

[0080] Comparative Example 4

[0081] Compared with Example 3, this comparative example is identical to Example 3 except that no Cyperus rotundus is added. All other raw materials and steps are the same as in Example 3.

[0082] Comparative Example 5

[0083] Compared with Example 3, this comparative example is identical to Example 3 except that water hyacinth is not added.

[0084] Comparative Example 6

[0085] Compared with Example 3, this comparative example is identical to Example 3 except that mulberry branches are not added.

[0086] Performance testing

[0087] Pot experiment:

[0088] The bok choy variety tested was "Four Seasons Bok Choy" from Shouhe Seed Industry.

[0089] The experiment consisted of 10 treatment groups: a blank control (CK, no fertilizer) and treatment groups T1-T9, which were treated with the organic fertilizers described in Examples 1-3 and Comparative Examples 1-6 of this invention. Only basal fertilizer was applied during the growth stage of the pakchoi. Organic fertilizer was applied at a mass ratio of 1:20 to soil. 3 kg of soil was weighed per pot, and the fertilizer and soil were mixed evenly before being placed in the pots. Ten pots were used for each treatment. Germinated pakchoi seeds were sown on the soil surface and watered. Thinning was performed when the seeds germinated and had three true leaves, leaving four pakchoi plants per pot. Uniform management measures were adopted during the growth period of the pakchoi.

[0090] Plant collection and determination of basic indicators

[0091] SPAD value determination: 20 days after setting the value, 6 Chinese cabbage plants were randomly selected from each treatment group, and the chlorophyll content was measured using a handheld chlorophyll meter (BERC-502).

[0092] At harvest, 10 Chinese cabbage plants with similar growth in each treatment group were selected to measure their plant height, leaf length, and leaf width, and their dry and fresh weight was also measured. The above-ground and underground parts of the Chinese cabbage were weighed separately using an electronic balance, and they were placed in a 105℃ oven for blanching for 15 minutes. The temperature was then adjusted to 70℃ and dried until constant weight was achieved, and the weight was then measured.

[0093] Quality index determination: Nitrate content of Chinese cabbage was determined by salicylic acid colorimetric method, vitamin C content was determined by 2,6-dichlorophenolindophenol titration method, soluble sugar content was determined by saccharimeter, and soluble solids content was determined by refractometer method.

[0094] Table 1 Growth indicators of pak choi under different treatments

[0095] Plant height (cm) Leaf length (cm) Leaf width (cm) SPAD value Fresh weight (g) CK 22.4 8.6 6.2 38 7.6 Example 1 32.2 14.7 11.2 55 20.7 Example 2 32.7 14.9 11.5 57 21.9 Example 3 33.1 15.2 11.7 58 22.5 Comparative Example 1 28.6 9.8 9.7 49 14.7 Comparative Example 2 27.9 10.5 8.5 45 12.4 Comparative Example 3 25.8 10.4 9.2 42 9.8 Comparative Example 4 31.5 12.7 9.6 55 19.2 Comparative Example 5 31.3 12.4 10.5 52 17.8 Comparative Example 6 30.8 11.8 10.1 51 16.3

[0096] As shown in Table 1, compared with the blank control, the plant height, leaf length, and leaf width of pakchoi increased to varying degrees after applying the organic fertilizer prepared in Examples 1-3 of this invention, and the fresh weight of pakchoi also increased significantly, indicating that the organic fertilizer of this invention can significantly promote the growth of pakchoi and increase biomass. Furthermore, compared with the blank control, the chlorophyll content in the leaves of pakchoi increased significantly after applying the organic fertilizer of this invention. However, after applying comparative examples 1-6 with altered components of this invention, all growth indicators of pakchoi decreased to varying degrees compared with the blank control, indicating that the effectiveness of the organic fertilizer of this invention is weakened if any component is missing.

[0097] Table 2 Quality Indicators of Bok Choy under Different Treatments

[0098]

[0099] As shown in Table 2, compared with the blank control, the content of soluble sugar, vitamin C and soluble solids increased after applying the organic fertilizer of the present invention. In addition, the nitrate content of the bok choy decreased, indicating that the application of the organic fertilizer of the present invention can improve the quality of bok choy.

[0100] It should be noted that the above embodiments are merely some preferred embodiments of the present invention, and not all embodiments. Obviously, based on the above embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort should fall within the scope of protection of the present invention.

Claims

1. An organic fertilizer based on biomass fermentation, characterized by, The preparation method of the organic fertilizer based on biomass fermentation comprises the following steps: (1) preparation of the compound microbial agent: (2) preparation of the fermented products of Cyperus rotundus, mulberry branches and water hyacinth: The Paenibacillus jellisii, Flavobacterium indicum and Stenotrophomonas rhizophila were respectively cultured in seed culture medium at temperatures of 28℃, 30℃ and 30℃ for 48 hours, and then inoculated into LB culture medium at a ratio of 1%, and cultured until the OD 600 = 3.0, and then mixed uniformly at a mass ratio of 1:1:1 to obtain a composite microbial inoculant. The Cyperus rotundus, water hyacinth and mulberry branches are dried and crushed to obtain a mixed powder, which is sieved through an 80-mesh screen, subjected to microwave treatment for 5 minutes, and subjected to enzymatic hydrolysis at 45-55 DEG C for 6-8 hours by adding a compound hydrolytic enzyme solution containing cellulase, xylanase and beta-glucosidase, and then inactivated at high temperature, and cooled to room temperature, and the mixture is prepared; the compound microbial agent is added to the mixture, and fermentation is carried out at 30 DEG C for 24-48 hours; (3) fermentation and maturation: The chicken manure, rapeseed cake, seaweed powder, corn straw and mushroom residue are mixed, dried in a ventilated manner until the water content is less than 5%, crushed through an 80-mesh screen, uniformly mixed by stirring, added into a fermentation tank, and adjusted to have a water content of 40-50%; while the fermentation substrate is stirred, the EM bacterial liquid is sprayed thereon so as to uniformly spray the EM bacterial liquid on the fermentation substrate, and then the fermentation culture is ventilated for 48 hours; the fermented products of Cyperus rotundus, mulberry branches and water hyacinth prepared in step (2) are added, uniformly mixed by stirring, and the fermentation tank is sealed with a plastic film; the upper layer is compacted with soil; the fermentation tank is turned over every 5 days during the period until the fermentation is completed; the plastic film is opened to obtain the fermented organic matter; (4) granulation: The fermented organic matter obtained in step (3) is dried in a ventilated manner until the water content is less than or equal to 20%, granulated by using a disc granulator, and controlled to have a particle size of 3-5 mm; the granules are dried by using a drying machine until the water content is less than or equal to 8%; the granules are naturally cooled to room temperature to obtain the final product, i.e., the organic fertilizer based on biomass fermentation. The organic fertilizer based on biomass fermentation is used for promoting the growth of Chinese cabbage and improving the quality of Chinese cabbage. In step (2) of the preparation method of the organic fertilizer based on biomass fermentation, the mass-volume ratio of the mixed powder of Cyperus rotundus, water hyacinth and mulberry branches to the compound hydrolytic enzyme is 1 kg: (1.5-2.0) L.

2. The organic fertilizer based on biomass fermentation according to claim 1, characterized by, The compound hydrolytic enzyme contains cellulase 16-18 g / L, xylanase 8-12 g / L and beta-glucosidase 14-22 g / L.

3. The organic fertilizer based on biomass fermentation according to claim 2, characterized by, ​

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