Device for simulating acute kidney injury

Abstract

The invention discloses a device for simulating acute kidney injury, and relates to the technical field of medical simulation devices.The device comprises a kidney simulation cavity and a control line, and a renal artery tube, a renal vein tube and a ureter are fixedly installed on the outer portion, close to the front portion, of the kidney simulation cavity side by side; one end of the renal artery tube, one end of the renal vein tube and one end of the ureter are fixedly connected with liquid storage bins, electromagnetic valves are fixedly installed at the positions, close to one ends, of the exteriors of the renal artery tube, the renal vein tube and the ureter, a control module is fixedly installed at the top of each liquid storage bin, and a connecting line is arranged at the top of each control module; a glomerulus simulation cavity, a renal interstitial substance simulation cavity and a renal tubule simulation cavity are fixedly mounted in the kidney simulation cavity in sequence. According to the device for simulating the acute kidney injury, a safe experimental platform is provided, and medical students and medical professionals can conveniently simulate development of the acute kidney injury under different conditions and observe various index data.

Description

Technical Field

[0001] The present invention relates to the technical field of medical simulation devices, and particularly relates to a device for simulating acute kidney injury. Background Art

[0002] Acute kidney injury refers to a disease state in which renal function rapidly declines within a short period of time (usually within a few hours to a few days). This injury can be caused by various reasons, including reduced blood supply to the kidneys, damaged renal tubules, abnormal glomerular function, etc.;

[0003] However, existing simulation devices cannot well simulate and observe acute kidney injury under various different etiological conditions, which is not convenient for medical students and medical professionals to deeply understand the development process of acute kidney injury, and it is difficult to deeply observe and understand the drug treatment process under different acute kidney injury conditions, resulting in problems of low practicability and functionality. Summary of the Invention

[0004] The main object of the present invention is to provide a device for simulating acute kidney injury, which can effectively solve the technical problems in the background art.

[0005] To achieve the above object, the technical solution adopted by the present invention is as follows:

[0006] A device for simulating acute kidney injury, comprising a renal simulation cavity and a control line. At a position near the front of the outside of the renal simulation cavity, a renal artery tube, a renal vein tube, and a ureter are fixedly installed in parallel. One end of each of the renal artery tube, the renal vein tube, and the ureter is fixedly connected to a liquid storage chamber. Solenoid valves are fixedly installed at a position near one end of the outside of the renal artery tube, the renal vein tube, and the ureter. A control module is fixedly installed at the top of the liquid storage chamber, and a connection line is arranged at the top of the control module;

[0007] Inside the renal simulation cavity, a glomerular simulation cavity, a renal interstitial simulation cavity, and a renal tubule simulation cavity are fixedly installed in sequence. Connection pipelines are provided between the glomerular simulation cavity and the renal interstitial simulation cavity and between the renal interstitial simulation cavity and the renal tubule simulation cavity. A microfluidic chip is arranged inside each connection pipeline, and the microfluidic chip is communicatively connected to an external computer through a control line. Pressure sensors are fixedly installed on the inner walls of the glomerular simulation cavity, the renal interstitial simulation cavity, the renal tubule simulation cavity, the inner wall of the renal artery tube, the inner wall of the renal vein tube, and the inner wall of the ureter. Flow sensors are arranged on the inner walls of the renal artery tube, the renal vein tube, and the ureter, and the flow sensors are communicatively connected to the control module.

[0008] As a further solution of the present invention, the three solenoid valves are respectively communicatively connected to three control modules, and the connection line is communicatively connected to an external computer.

[0009] As a further solution of the present invention, a liquid inlet pipe is fixedly installed near the bottom outside the liquid storage chamber connected to the renal artery tube, and liquid outlet pipes are fixedly installed near the bottom outside the other two liquid storage chambers. Water pumps are connected to the outside of both the liquid inlet pipe and the liquid outlet pipe.

[0010] As a further solution of the present invention, a water outlet pipe is fixedly installed through the bottom of the glomerular simulation cavity and through the bottom of the renal simulation cavity, and a ball valve is fixedly installed outside the water outlet pipe.

[0011] A method for simulating acute kidney injury, the specific steps are as follows:

[0012] S1. Inject simulated liquid into the liquid storage chamber connected to the renal artery tube through the liquid inlet pipe, control the control module through an external computer, set the reference state of the device, and obtain the reference parameter values.

[0013] S2. Collect medical data, combine with acute kidney injury caused by different etiologies, adjust the internal parameters of the device through an external computer to simulate different acute kidney injury situations, and record the morbidity data.

[0014] S3. Combine the collected medical data, summarize and organize the index data for each period, and combine with the prediction model to predict the development of acute kidney injury in different situations, and at the same time summarize the main index characteristics for different periods.

[0015] S4. Set a control group of diseased mice, conduct drug treatment on the control group of diseased mice based on the acute kidney injury simulated in the simulation cavity, obtain the filtration data of the renal tubules, glomeruli, and renal interstitium after medication, adjust the internal parameters of the simulation cavity to simulate the drug effect, combine the obtained data, adjust the internal parameters of the simulation cavity to simulate the situation after medication, and record the internal parameters of the renal simulation cavity after adjustment.

[0016] S5. Continuously treat the diseased mice simulating different acute kidney injuries, and based on the treatment results, simulate the overall medication treatment process of acute kidney injury.

[0017] As a further solution of the present invention, the step of injecting simulated liquid into the liquid storage chamber connected to the renal artery tube through the liquid inlet pipe, controlling the control module through an external computer, setting the reference state of the device, and obtaining the reference parameter values includes the following steps:

[0018] S11. Connect the liquid inlet pipe through a water pump and inject simulated liquid into the liquid storage chamber connected to the renal artery tube. Control the control module through a computer to open the solenoid valve, so that the simulated liquid enters the glomerular simulation cavity through the renal artery tube, and after the filtration effect of the connecting pipeline, it is discharged through the renal vein tube and the ureter to simulate the circulation process.

[0019] S12. Through an external computer, adjust the microfluidic chip inside the connecting pipeline, and use the microfluidic chip to restrict the simulated liquid flow between the glomerulus simulation cavity and the renal interstitial simulation cavity, and between the renal interstitial simulation cavity and the renal tubule simulation cavity, including the flow rate and the allowable filtration range, to simulate the work of the glomerulus, renal interstitium, and renal tubules.

[0020] S13. Collect the standard parameters of normal kidney operation. Based on the parameters, set the flow rate and pressure of the renal artery tube, and the control parameters of the microfluidic chip for the connecting pipeline through the computer as the reference operating parameters. And obtain the pressure values of the glomerulus simulation cavity, renal interstitial simulation cavity, and renal tubule simulation cavity through a pressure sensor and use them as the reference pressure values. Collect the reference flow rates of the renal artery tube, renal vein tube, and ureter under the reference state.

[0021] As a further solution of the present invention, for collecting medical data, in combination with acute kidney injury caused by different etiologies, adjust the internal parameters of the device through an external computer, simulate different acute kidney injury situations, and record the morbidity data, including the following steps:

[0022] S21. In combination with acute kidney injury caused by different etiologies, adjust the internal parameters of the device through the control module to simulate different acute kidney injury situations, including the following steps:

[0023] S211. Through an external computer, adjust the microfluidic chip to adjust the filtration range and flow rate between the glomerulus simulation cavity and the renal interstitial simulation cavity, and between the renal interstitial simulation cavity and the renal tubule simulation cavity, and keep the state of injecting the simulated liquid into the renal artery tube unchanged to simulate abnormal glomerular filtration function, impaired renal tubule reabsorption function, and glomerulonephritis.

[0024] Through an external computer, adjust the microfluidic chip to adjust the filtration range and flow rate between the glomerulus simulation cavity and the renal interstitial simulation cavity, and between the renal interstitial simulation cavity and the renal tubule simulation cavity, and reduce the flow rate of the simulated liquid injected into the renal artery tube to simulate the situation of acute renal failure.

[0025] S22. Analyze and record relevant information on the components of the simulated liquid flowing out through the liquid outlet tube during the circulation process, collect the pressure data in the simulation cavity through a pressure sensor, and continuously record relevant data to simulate the relevant information in different periods of the disease development, including the components of the discharged simulated liquid, the pressure in the simulation cavity, the pressure and flow rate of the renal vein tube, and the pressure and flow rate of the ureter.

[0026] As a further solution of the present invention, the combined collection of medical data is used to summarize and organize the index data for each period, and combined with a prediction model to predict the development of acute kidney injury in different situations. At the same time, the main index characteristics in different periods are summarized, including the following steps:

[0027] S31. Sort and classify the collected relevant data, including simulated liquid components, simulated cavity filtration range and flow rate, renal vein tube pressure and flow rate, ureteral pressure and flow rate. Through statistical methods, extract the characteristic parameters in the relevant data and generate a line graph, including glomerular filtration rate, tubular filtration rate, simulated urine volume, simulated liquid proteinuria degree, glomerular simulated cavity pressure, renal interstitial simulated cavity pressure, and tubular simulated cavity pressure;

[0028] S32. Combine the glomerular filtration rate, tubular filtration rate, simulated urine volume, simulated liquid proteinuria degree, glomerular simulated cavity pressure, renal interstitial simulated cavity pressure, and tubular simulated cavity pressure to generate a multiple regression model to predict the subsequent development of acute kidney injury. The specific algorithm formula is:

[0029]

[0030] Among them, θ represents the target variable, that is, the degree of acute kidney injury, β0 represents the intercept of the model, to respectively represent the characteristics of glomerular filtration rate, tubular filtration rate, simulated urine volume, simulated liquid proteinuria degree, glomerular simulated cavity pressure, renal interstitial simulated cavity pressure, and tubular simulated cavity pressure. β1 to β7 respectively represent the influence of the corresponding characteristics on the target variable, and θ is the error term;

[0031] S33. Statistical analysis is used to determine the influence of different indicators on the development of acute kidney injury in different periods and generate charts.

[0032] As a further solution of the present invention, a control group of diseased mice is set up. Based on the simulated acute kidney injury in the simulated cavity, drug treatment is carried out on the control group of diseased mice, and the filtration data of the renal tubules, glomeruli, and renal interstitium after medication are obtained, and the internal parameters of the simulated cavity are adjusted to simulate the drug effect, including the following steps:

[0033] S41. Set up a control group of diseased mice, and use drug induction and surgical operations to make the mice simulate acute kidney injury. At the same time, collect the internal information of the diseased mice's kidneys, including glomerular filtration rate and tubular filtration rate;

[0034] S42. Adjust the microfluidic chip according to the internal information of the kidneys of diseased mice, and adjust the filtration range and flow rate between the glomerulus simulation chamber and the renal interstitial simulation chamber, and between the renal interstitial simulation chamber and the renal tubule simulation chamber;

[0035] S43. Treat the acute kidney injury of diseased mice with drugs, and record the relevant parameters during the treatment process, including the glomerular filtration rate and the renal tubule filtration rate. Based on the relevant parameters during the treatment process, adjust the microfluidic chip through an external computer, and record the pressure changes in the simulation chamber, renal artery tube, renal vein tube, and ureter, as well as the parameters of the discharged simulated liquid.

[0036] As a further solution of the present invention, continuously treat the diseased mice simulating different acute kidney injuries, and based on the treatment results, simulate the overall medication treatment process of acute kidney injury. The specific steps are as follows:

[0037] S51. Continuously collect the drug usage data of diseased mice in different simulation situations to obtain the filtration data of renal tubules, glomeruli, and renal interstitium at different medication stages;

[0038] S52. Generate a data line graph based on the filtration data of renal tubules, glomeruli, and renal interstitium at different medication stages;

[0039] S53. Based on the data line graph, adjust the relevant parameters through the microfluidic chip and the control module to simulate the overall medication treatment process of acute kidney injury.

[0040] The beneficial effects of the present invention are as follows:

[0041] By setting up each simulation chamber and adjusting the filtration range and flow rate of each simulation chamber through an external computer, it is possible to simulate acute kidney injury caused by different situations, which is convenient for medical teaching and research on acute kidney injury, helps medical students and medical professionals understand the occurrence mechanism and pathophysiological changes of acute kidney injury, and enhances the practicality of the device;

[0042] By summarizing and organizing the index data in each period during the simulation process and generating a prediction model to predict the subsequent development process of acute kidney injury, it is possible to help identify the risk factors and development trends of acute kidney injury patients, enabling medical staff to identify, intervene, and treat patients at an early stage, thereby reducing the possibility of the condition worsening and improving the medical value of the device;

[0043] By combining the data of the control group of diseased mice, a line graph of treatment parameters is generated. By combining the line graph and adjusting the internal parameters of the device through an external computer, during the treatment process of acute kidney injury under different conditions, it is convenient for medical students and medical professionals to observe the changes in treatment effects, thereby enhancing practical operation capabilities.

[0044] The simulation device provides a safe experimental platform, facilitating medical students and medical professionals to perform practical operations in a simulated environment, improving their ability to handle the treatment of acute kidney injury. Moreover, the simulation device can be used both as a tool for medical teaching and for scientific research purposes, enhancing the functionality of the device. Brief Description of the Drawings

[0045] Figure 1 It is a schematic diagram of the overall structure of a device for simulating acute kidney injury according to the present invention;

[0046] Figure 2 It is a schematic diagram of a partial section of a device for simulating acute kidney injury according to the present invention;

[0047] Figure 3 It is a flowchart of a method for simulating acute kidney injury according to the present invention.

[0048] In the figure: 1, renal simulation cavity; 2, renal artery tube; 3, renal vein tube; 4, ureter; 5, liquid storage bin; 6, liquid inlet tube; 7, liquid outlet tube; 8, solenoid valve; 9, control module; 10, connection line; 11, glomerulus simulation cavity; 12, renal interstitial simulation cavity; 13, renal tubule simulation cavity; 14, connecting pipeline; 15, pressure sensor; 16, control line. Detailed Embodiments

[0049] To make the technical means, creative features, achieved purposes, and effects of the present invention easy to understand, the present invention will be further described below in conjunction with specific embodiments.

[0050] As Figures 1-3 shown, a device for simulating acute kidney injury includes a renal simulation cavity 1 and a control line 16. The renal artery tube 2, renal vein tube 3, and ureter 4 are fixedly installed in parallel near the front position outside the renal simulation cavity 1. One end of each of the renal artery tube 2, renal vein tube 3, and ureter 4 is fixedly connected to a liquid storage bin 5. Solenoid valves 8 are fixedly installed near one end outside the renal artery tube 2, renal vein tube 3, and ureter 4. A control module 9 is fixedly installed on the top of the liquid storage bin 5, and a connection line 10 is arranged on the top of the control module 9;

[0051] Inside the renal simulation cavity 1, a glomerulus simulation cavity 11, a renal interstitial simulation cavity 12, and a renal tubule simulation cavity 13 are successively and fixedly installed. Connecting pipelines 14 are provided between the glomerulus simulation cavity 11 and the renal interstitial simulation cavity 12, and between the renal interstitial simulation cavity 12 and the renal tubule simulation cavity 13. A microfluidic chip is arranged inside each connecting pipeline 14, and the microfluidic chip is communicatively connected to an external computer through a control line 16. Pressure sensors 15 are fixedly installed on the inner walls of the glomerulus simulation cavity 11, the renal interstitial simulation cavity 12, the renal tubule simulation cavity 13, as well as the inner walls of the renal artery tube 2, the renal vein tube 3, and the ureter 4. Flow sensors are arranged on the inner walls of the renal artery tube 2, the renal vein tube 3, and the ureter 4, and the flow sensors are communicatively connected to a control module 9.

[0052] In this embodiment, three electromagnetic valves 8 are respectively communicatively connected to three control modules 9, and a connecting line 10 is communicatively connected to an external computer.

[0053] The three electromagnetic valves 8 are respectively communicatively connected to the three control modules 9. The control module 9 can be controlled through an external computer to control the electromagnetic valves 8, which is convenient for simulating the process of acute kidney injury.

[0054] In this embodiment, a liquid inlet pipe 6 is fixedly installed near the bottom outside the liquid storage bin 5 connected to the renal artery tube 2, and liquid outlet pipes 7 are fixedly installed near the bottom outside the other two liquid storage bins 5. Water pumps are connected to the outside of the liquid inlet pipe 6 and the liquid outlet pipes 7.

[0055] The simulated liquid enters the renal artery tube 2 through the liquid inlet pipe, and enters the glomerulus simulation cavity 11 through the electromagnetic valve 8. After the simulated filtration, the remaining simulated liquid is discharged through the renal vein tube and the ureter.

[0056] In this embodiment, a water outlet pipe is fixedly installed through the bottom of the glomerulus simulation cavity 11 and the bottom of the renal simulation cavity 1, and a ball valve is fixedly installed outside the water outlet pipe.

[0057] It should be noted that the experimenter controls the water pump through an external computer to send the simulated liquid into the liquid storage bin 5 through the liquid inlet pipe 6, controls the electromagnetic valve 8 to open through the control module 9, and sets the microfluidic chip inside the connecting pipeline 14 through the external computer to set the filtration range and flow rate for simulating acute kidney injury. The simulated liquid first enters the glomerulus simulation cavity 11, is filtered into the renal interstitial simulation cavity 12, and finally filtered into the renal tubule simulation cavity 13. The simulated liquid in the renal interstitial simulation cavity 12 and the renal tubule simulation cavity 13 will enter the corresponding liquid storage bin 5 through the renal vein tube 3 and the ureter 4, and the data is recorded through the flow sensors and pressure sensors;

[0058] By adjusting the flow rate, flow volume of the simulated liquid, as well as the filtration range and flow rate, the situation of acute kidney injury under different conditions is simulated. After the simulation is completed, by opening the ball valve of the water outlet pipe, the residual liquid in the glomerular simulation cavity 11 is discharged to facilitate subsequent simulations.

[0059] A method for simulating acute kidney injury, comprising the following steps:

[0060] S1. Inject the simulated liquid into the liquid storage bin 5 connected to the renal artery tube 2 through the liquid inlet pipe 6. Control the control module 9 through an external computer to set the reference state of the device and obtain the reference parameter values;

[0061] S11. Connect the water pump to the liquid inlet pipe 6 and inject the simulated liquid into the liquid storage bin 5 connected to the renal artery tube 2. Control the control module 9 through the computer to open the solenoid valve 8, so that the simulated liquid enters the glomerular simulation cavity 11 through the renal artery tube 2, and after passing through the filtration function of the connecting pipeline 14, it is discharged through the renal vein tube 3 and the ureter 4 to simulate the circulation process;

[0062] S12. Through an external computer, adjust the microfluidic chip inside the connecting pipeline 14, and limit the flow of the simulated liquid between the glomerular simulation cavity 11 and the renal interstitial simulation cavity 12, and between the renal interstitial simulation cavity 12 and the renal tubule simulation cavity 13 through the microfluidic chip, including the flow rate and the allowable filtration range, to simulate the work of the glomerulus, renal interstitium, and renal tubules;

[0063] S13. Collect the standard parameters of normal kidney operation, set the flow rate, pressure of the renal artery tube 2, and the control parameters of the microfluidic chip for the connecting pipeline 14 based on the parameters through the computer as the reference operation parameters, and obtain the pressure values of the glomerular simulation cavity 11, the renal interstitial simulation cavity 12, and the renal tubule simulation cavity 13 through the pressure sensor and use them as the reference pressure values, and collect the reference flow rates of the renal artery tube 2, the renal vein tube 3, and the ureter 4 under the reference state.

[0064] It should be noted that by setting the reference parameters, the subsequent parameters can be adjusted based on the reference parameters to achieve the simulation of different acute kidney injuries and monitor and compare the indicators in the simulation experiment state.

[0065] In this embodiment, S2. Collect medical data, combine acute kidney injuries caused by different etiologies, adjust the internal parameters of the device through an external computer to simulate different acute kidney injury situations, and record the morbidity data;

[0066] S21. Combine acute kidney injuries caused by different etiologies and adjust the internal parameters of the device through the control module 9 to simulate different acute kidney injury situations, including the following steps:

[0067] S211. Adjust the microfluidic chip through an external computer to adjust the filtration range and flow rate between the glomerulus simulation chamber 11 and the renal interstitial simulation chamber 12, and between the renal interstitial simulation chamber 12 and the renal tubule simulation chamber 13, and keep the state of injecting the simulated liquid into the renal artery tube 2 unchanged to simulate abnormal glomerular filtration function, impaired renal tubule reabsorption function, and glomerulonephritis;

[0068] Adjust the microfluidic chip through an external computer to adjust the filtration range and flow rate between the glomerulus simulation chamber 11 and the renal interstitial simulation chamber 12, and between the renal interstitial simulation chamber 12 and the renal tubule simulation chamber 13, and reduce the flow rate of the simulated liquid injected into the renal artery tube 2 to simulate the situation of acute renal failure;

[0069] S22. Analyze and record relevant information on the components of the simulated liquid flowing out through the liquid outlet tube during the circulation process, collect the pressure data in the simulation chamber through the pressure sensor 15, and continuously record relevant data to simulate the relevant information in different periods of the disease development, including the components of the discharged simulated liquid, the pressure in the simulation chamber, the pressure and flow rate of the renal vein tube, and the pressure and flow rate of the ureter.

[0070] It should be noted that: reducing the filtration range between the glomerulus simulation chamber 11 and the renal interstitial simulation chamber 12 can simulate the situation of glomerular filtration disorder, simulating the decline of glomerular filtration function caused by glomerular diseases, vascular lesions or other factors. Adjusting to reduce the filtration range between the renal interstitial simulation chamber 12 and the renal tubule simulation chamber 13 can simulate the situation of impaired renal tubule reabsorption function, including the decline of the renal tubule's reabsorption ability of water and electrolytes caused by renal tubule diseases, drug toxicity or other reasons. Adjusting to increase the filtration range between the renal interstitial simulation chamber 12 and the renal tubule simulation chamber 13 will lead to an increase in renal interstitial fluid, thereby increasing the renal interstitial pressure, simulating acute kidney injury in some cases, including renal ischemia-reperfusion injury or local renal ischemia caused by local renal vascular occlusion.

[0071] S3. Combine the collected medical data, summarize and organize the index data for each period, and combine with the prediction model to predict the development of acute kidney injury in different situations, and at the same time summarize the main index characteristics for different periods;

[0072] S31. Sort and classify the collected relevant data, including the components of the simulated liquid, the filtration range and flow rate of the simulation chamber, the pressure and flow rate of the renal vein tube, and the pressure and flow rate of the ureter. Extract the characteristic parameters from the relevant data through statistical methods and generate line charts, including glomerular filtration rate, renal tubule filtration rate, simulated urine volume, degree of simulated liquid proteinuria, pressure in the glomerulus simulation chamber 11, pressure in the renal interstitial simulation chamber 12, and pressure in the renal tubule simulation chamber 13;

[0073] S32. Combine the glomerular filtration rate, tubular filtration rate, simulated urine volume, simulated degree of liquid proteinuria, pressure in the glomerular simulation chamber 11, pressure in the renal interstitial simulation chamber 12, and pressure in the tubular simulation chamber 13 to generate a multiple regression model for predicting the subsequent development of acute kidney injury. The specific algorithm formula is as follows:

[0074]

[0075] Wherein, represents the target variable, that is, the degree of acute kidney injury, β0 represents the intercept of the model, to respectively represent the characteristics of glomerular filtration rate, tubular filtration rate, simulated urine volume, simulated degree of liquid proteinuria, pressure in the glomerular simulation chamber 11, pressure in the renal interstitial simulation chamber 12, and pressure in the tubular simulation chamber 13. β1 to β7 respectively represent the influence of the corresponding characteristics on the target variable, and θ is the error term;

[0076] S33. Statistical analysis is used to determine the influence of different indicators on the development of acute kidney injury at different times and generate charts.

[0077] It should be noted that predicting the development of acute kidney injury under different conditions and analyzing the differences and associations between the predicted results and the actual observed results can help trainees deeply understand the pathophysiological process and development mechanism of acute kidney injury.

[0078] S4. Set up a control group of diseased mice. Based on the simulated acute kidney injury in the simulation chamber, drug treatment is carried out on the control group of diseased mice, and the filtration data of the renal tubules, glomeruli, and renal interstitium after drug administration are obtained. The internal parameters of the simulation chamber are adjusted to simulate the drug effect. Combining the obtained data, the internal parameters of the simulation chamber are adjusted to simulate the situation after drug administration, and the adjusted internal parameters of the renal simulation chamber are recorded;

[0079] S41. Set up a control group of diseased mice, and use drug induction and surgical operations to make the mice simulate acute kidney injury. At the same time, collect the internal information of the kidneys of the diseased mice, including glomerular filtration rate and tubular filtration rate;

[0080] S42. According to the internal information of the kidneys of the diseased mice, adjust the microfluidic chip, and adjust the filtration range and flow rate between the glomerular simulation chamber 11 and the renal interstitial simulation chamber 12, and between the renal interstitial simulation chamber 12 and the tubular simulation chamber 13;

[0081] S43. Treat the acute kidney injury of diseased mice with drugs, and record the relevant parameters during the treatment process, including the glomerular filtration rate and the tubular filtration rate. Based on the relevant parameters during the treatment process, adjust the microfluidic chip through an external computer, and record the pressure changes in the simulation chamber, renal artery tube, renal vein tube, and ureter, as well as the parameters of the discharged simulated liquid.

[0082] S5. Continuously treat diseased mice simulating different acute kidney injuries, and based on the treatment results, simulate the overall drug treatment process for acute kidney injury.

[0083] S51. Continuously collect the drug usage data of diseased mice in different simulation situations to obtain the filtration data of the renal tubules, glomeruli, and renal interstitium at different drug treatment stages.

[0084] S52. Generate a data line graph based on the filtration data of the renal tubules, glomeruli, and renal interstitium at different drug treatment stages.

[0085] S53. Based on the data line graph, adjust the relevant parameters through the microfluidic chip and the control module 9 to simulate the overall drug treatment process for acute kidney injury.

[0086] It should be noted that by combining the drug usage of the diseased mouse control group and simulating the treatment result data, the treatment schemes for different acute kidney injuries can be simulated by this device, which is convenient for trainees to learn and summarize the treatment of acute kidney injury.

[0087] The above shows and describes the basic principles, main features, and advantages of the present invention. Those skilled in the art of this industry should understand that the present invention is not limited by the above embodiments. What is described in the above embodiments and the specification only illustrates the principle of the present invention. Without departing from the spirit and scope of the present invention, the present invention will have various changes and improvements, and these changes and improvements all fall within the scope of the present invention claimed.

Claims

1. An apparatus for simulating acute kidney injury, comprising a kidney simulation cavity (1) and a control line (16), characterized in that, Outside the kidney simulation cavity (1) and near the front position, a renal artery tube (2), a renal vein tube (3), and a ureter (4) are fixedly installed in parallel. One end of each of the renal artery tube (2), the renal vein tube (3), and the ureter (4) is fixedly connected to a liquid storage chamber (5). Solenoid valves (8) are fixedly installed near one end of the outside of the renal artery tube (2), the renal vein tube (3), and the ureter (4). A control module (9) is fixedly installed on the top of the liquid storage chamber (5), and a connecting line (10) is arranged on the top of the control module (9); Inside the kidney simulation cavity (1), a glomerulus simulation cavity (11), a renal interstitial simulation cavity (12), and a renal tubule simulation cavity (13) are fixedly installed in sequence. Connecting pipelines (14) are provided between the glomerulus simulation cavity (11) and the renal interstitial simulation cavity (12) and between the renal interstitial simulation cavity (12) and the renal tubule simulation cavity (13). A microfluidic chip is arranged inside each connecting pipeline (14), and the microfluidic chip is communicatively connected to an external computer through a control line (16). Pressure sensors (15) are fixedly installed on the inner walls of the glomerulus simulation cavity (11), the renal interstitial simulation cavity (12), the renal tubule simulation cavity (13), the inner wall of the renal artery tube (2), the inner wall of the renal vein tube (3), and the inner wall of the ureter (4). Flow sensors are arranged on the inner walls of the renal artery tube (2), the renal vein tube (3), and the ureter (4), and the flow sensors are communicatively connected to the control module (9).

2. The device for simulating acute kidney injury according to claim 1, wherein: The three solenoid valves (8) are respectively communicatively connected to the three control modules (9), and the connecting line (10) is communicatively connected to an external computer.

3. The device for simulating acute kidney injury according to claim 1, wherein: A liquid inlet pipe (6) is fixedly installed near the bottom of the outside of the liquid storage chamber (5) connected to the renal artery tube (2), and liquid outlet pipes (7) are fixedly installed near the bottom of the outside of the other two liquid storage chambers (5). Water pumps are connected to the outside of the liquid inlet pipe (6) and the liquid outlet pipes (7).

4. The device for simulating acute kidney injury according to claim 1, wherein: A water outlet pipe is fixedly installed through the bottom of the glomerulus simulation cavity (11) and the bottom of the kidney simulation cavity (1), and a ball valve is fixedly installed on the outside of the water outlet pipe.

5. A method for simulating acute kidney injury, characterized in that, This method uses a device for simulating acute kidney injury as described in any one of claims 1-4, and the specific steps are as follows: S1. Inject simulated liquid into the liquid storage chamber (5) connected to the renal artery tube (2) through the liquid inlet pipe (6). Control the control module (9) through an external computer, set the reference state of the device, and obtain the reference parameter values; S2. Collect medical data, combine acute kidney injuries caused by different etiologies, adjust the internal parameters of the device through an external computer to simulate different acute kidney injury situations, and record the morbidity data; S3. Combine the collected medical data, summarize and organize the index data for each period, and combine with a prediction model to predict the development of acute kidney injury in different situations, and summarize the main index characteristics in different periods; S4. Set up a control group of diseased mice. Based on the simulated acute kidney injury in the simulation chamber, perform drug treatment on the control group of diseased mice, and obtain the filtration data of renal tubules, glomeruli, and renal interstitium after drug administration. Adjust the internal parameters of the simulation chamber to simulate the drug effect. Combine the obtained data to adjust the internal parameters of the simulation chamber, simulate the situation after drug administration, and record the adjusted internal parameters of the renal simulation chamber. S5. Continuously treat the diseased mice simulating different acute kidney injuries, and based on the treatment results, simulate the overall drug treatment process of acute kidney injury.

6. A method for simulating acute kidney injury according to claim 5, characterized in that: Inject simulated liquid into the liquid storage chamber (5) connected to the renal artery tube (2) through the liquid inlet tube (6). Control the control module (9) through an external computer to set the reference state of the device and obtain the reference parameter values, including the following steps: S11. Connect the liquid inlet tube (6) through a water pump and inject simulated liquid into the liquid storage chamber (5) connected to the renal artery tube (2). Open the solenoid valve (8) through the computer to control the control module (9), so that the simulated liquid enters the glomerular simulation chamber (11) through the renal artery tube (2), and after passing through the filtration effect of the connecting pipeline (14), it is discharged through the renal vein tube (3) and the ureter (4) to simulate the circulation process. S12. Adjust the microfluidic chip inside the connecting pipeline (14) through an external computer. Use the microfluidic chip to limit the flow of simulated liquid between the glomerular simulation chamber (11) and the renal interstitial simulation chamber (12), and between the renal interstitial simulation chamber (12) and the renal tubule simulation chamber (13), including the flow rate and the allowable filtration range, to simulate the work of glomeruli, renal interstitium, and renal tubules. S13. Collect the standard parameters of normal kidney operation. Based on the parameters, set the flow rate and pressure of the renal artery tube (2) through the computer, as well as the control parameters of the microfluidic chip for the connecting pipeline (14) as the reference operation parameters. Obtain the pressure values of the glomerular simulation chamber (11), the renal interstitial simulation chamber (12), and the renal tubule simulation chamber (13) through the pressure sensor and use them as the reference pressure values. Collect the reference flow rates of the renal artery tube (2), the renal vein tube (3), and the ureter (4) under the reference state.

7. A method for simulating acute kidney injury according to claim 6, characterized in that: The collection of medical data, combined with acute kidney injury caused by different etiologies, adjusts the internal parameters of the device through an external computer to simulate different acute kidney injury situations and records the morbidity data, including the following steps: S21. Combine acute kidney injury caused by different etiologies and adjust the internal parameters of the device through the control module (9) to simulate different acute kidney injury situations, including the following steps: S211. Adjust the microfluidic chip through an external computer to adjust the filtration range and flow rate between the glomerular simulation chamber (11) and the renal interstitial simulation chamber (12), and between the renal interstitial simulation chamber (12) and the renal tubule simulation chamber (13), and keep the state of injecting simulated liquid into the renal artery tube (2) unchanged to simulate abnormal glomerular filtration function, impaired renal tubule reabsorption function, and glomerulonephritis. Adjust the microfluidic chip through an external computer to adjust the filtration range and flow rate between the glomerular simulation cavity (11) and the renal interstitial simulation cavity (12), and between the renal interstitial simulation cavity (12) and the renal tubule simulation cavity (13), and reduce the flow rate of the simulated liquid injected into the renal artery tube (2) to simulate the situation of acute renal failure; S22. Analyze and record relevant information on the components of the simulated liquid flowing out through the liquid outlet tube during the circulation process, collect the pressure data in the simulation cavity through the pressure sensor (15), and continuously record relevant data to simulate the relevant information in different periods of the disease development, including the components of the discharged simulated liquid, the pressure in the simulation cavity, the pressure and flow rate of the renal vein tube, and the pressure and flow rate of the ureter.

8. A method for simulating acute kidney injury according to claim 6, characterized in that: Combined with the collection of medical data, summarize and organize the index data for each period, and combined with the prediction model, predict the development of acute kidney injury in different situations, and summarize the main index characteristics in different periods, including the following steps: S31. Sort and classify the collected relevant data, including the components of the simulated liquid, the filtration range and flow rate of the simulation cavity, the pressure and flow rate of the renal vein tube, and the pressure and flow rate of the ureter. Extract the characteristic parameters from the relevant data through statistical methods and generate a line chart, including the glomerular filtration rate, the renal tubule filtration rate, the simulated urine volume, the degree of proteinuria in the simulated liquid, the pressure in the glomerular simulation cavity (11), the pressure in the renal interstitial simulation cavity (12), and the pressure in the renal tubule simulation cavity (13); S32. Combine the glomerular filtration rate, the renal tubule filtration rate, the simulated urine volume, the degree of proteinuria in the simulated liquid, the pressure in the glomerular simulation cavity (11), the pressure in the renal interstitial simulation cavity (12), and the pressure in the renal tubule simulation cavity (13) to generate a multiple regression model to predict the subsequent development of acute kidney injury. The specific algorithm formula is: Among them, θ represents the target variable, that is, the degree of acute kidney injury, β0 represents the intercept of the model, to respectively represent the glomerular filtration rate, the tubular filtration rate, the simulated urine volume, the degree of simulated liquid proteinuria, the pressure in the glomerular simulation cavity (11), the pressure in the renal interstitial simulation cavity (12), the pressure characteristics in the tubular simulation cavity (13), β1 to β7 respectively represent the influence of the corresponding characteristics on the target variable, and θ is the error term; S33. Statistically analyze and determine the influence of different indicators on the development of acute kidney injury in different periods and generate a chart.

9. A method for simulating acute kidney injury according to claim 5, characterized in that: Set up a control group of diseased mice, treat the acute kidney injury simulated in the simulation cavity for the control group of diseased mice with drugs, and obtain the filtration data of the renal tubules, glomeruli, and renal interstitium after drug administration, and adjust the internal parameters of the simulation cavity to simulate the drug effect, including the following steps: S41. Set up a control group of diseased mice, and use drug induction and surgical operations to make the mice simulate acute kidney injury. At the same time, collect the internal information of the kidneys of the diseased mice, including the glomerular filtration rate and the renal tubule filtration rate; S42. Adjust the microfluidic chip according to the internal information of the kidneys of the diseased mice, and adjust the filtration range and flow rate between the glomerular simulation cavity (11) and the renal interstitial simulation cavity (12), and between the renal interstitial simulation cavity (12) and the renal tubule simulation cavity (13); S43. Treat the acute kidney injury of diseased mice with drugs, and record the relevant parameters during the treatment, including the glomerular filtration rate and the tubular filtration rate. Based on the relevant parameters during the treatment, adjust the microfluidic chip through an external computer, and record the pressure changes in the simulation chamber, renal artery tube, renal vein tube, and ureter, as well as the parameters of the discharged simulated liquid.

10. A method for simulating acute kidney injury according to claim 5, characterized in that: Continuously treat the diseased mice simulating different acute kidney injuries, and based on the treatment results, simulate the overall drug treatment process for acute kidney injury. The specific steps are as follows: S51. Continuously collect the drug usage data of the diseased mice in different simulation situations to obtain the filtration data of the renal tubules, glomeruli, and renal interstitium at different drug usage stages; S52. Generate a data line graph based on the filtration data of the renal tubules, glomeruli, and renal interstitium at different drug usage stages; S53. Based on the data line graph, adjust the relevant parameters through the microfluidic chip and the control module (9) to simulate the overall drug treatment process for acute kidney injury.

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