Feed containing glycerol monolaurate and preparation method thereof

Abstract

The invention relates to the technical field of feeds, in particular to a feed containing glycerol monolaurate and a preparation method of the feed. Comprising the following components: 55-65 parts of corn, 1-10 parts of corn distillers' grains, 10-15 parts of wheat, 12-18 parts of soybean meal, 1-3 parts of coconut oil, 0.5-1.5 parts of fish oil, 3-5 parts of a plant extract, 0.1-0.5 part of glycerol monolaurate, 1-2 parts of a functional additive, 0.5-1 part of a premix and 1-1.5 parts of amino acid. According to the invention, the glycerol monolaurate, the modified montmorillonite-sodium butyrate compound, the plant extract and the amino acid are combined in the basal feed to construct a multi-level intestinal health regulation and control system, and the feed system shows the effects of remarkably reducing the incidence rate of diarrhea and improving the daily gain in a pig feeding experiment.

Description

Technical Field

[0001] The present invention relates to the technical field of feeds, and particularly to a feed containing monolaurin and a preparation method thereof. Background Art

[0002] As an important input in pig production, pig feed not only needs to meet the basic nutritional needs of pigs, but also ensure the healthy growth of pigs and the best production performance. With the continuous expansion of the intensive farming scale, the stress of pigs increases and the intestinal health problems are prominent, which directly affect the growth performance of pigs and the breeding efficiency. High-quality pig feed needs to comprehensively consider the balance of nutrients such as energy, protein, essential amino acids, minerals, and vitamins, and at the same time should have multiple functions such as improving intestinal health, enhancing immunity, and reducing disease occurrence. With the full implementation of the feed additive antibiotic ban policy, the development of safe, efficient, and green functional feeds has become an important direction in current pig feed research.

[0003] CN202211304435.6 discloses a probiotic feed for improving intestinal health and removing odors, and its composition includes: 50-55 parts of corn flour, 11-20 parts of fish meal, 25-31 parts of soybean meal, 10-15 parts of brown sugar, 5-7 parts of probiotic bacteria, 1-2 parts of enzyme preparation, 6-9 parts of vitamin-mineral premix, and 0.2-0.6 parts of additive; among them, the probiotic bacteria include Enterococcus faecalis, Bacillus subtilis, Bacillus licheniformis, and Saccharomyces cerevisiae, and the additive includes components such as mulberry leaves, Coptis chinensis, betaine, and saponin. Although this technology can promote the reproduction of beneficial bacteria and inhibit the activities of harmful bacteria to a certain extent, there are still obvious deficiencies: First, its effect of regulating the flora is relatively single, lacking a direct mechanism for repairing the intestinal mucosal barrier; Second, the composition of the additive is complex and the dosage is large, resulting in high costs and complex production processes; Third, some components in the additive such as Coptis chinensis have poor stability during processing and are easily inactivated by high temperature; In addition, this technology focuses more on reducing the odor of pig manure and the emission of harmful gases, and is insufficient in improving feed conversion rate and intestinal absorption function, and it is difficult to comprehensively improve the intestinal health level and growth performance of pigs. Therefore, there is an urgent need to develop a more efficient, economical, and stable feed formula to comprehensively improve the intestinal health of pigs. Summary of the Invention

[0004] In view of this, the present invention proposes a feed containing monolaurin and a preparation method thereof to solve the comprehensive technical problems of single flora regulation means, poor thermal stability of additives, and insufficient intestinal barrier repair mechanism in existing pig feeds.

[0005] The technical solution of the present invention is achieved as follows: The present invention provides a feed containing monolaurin, which comprises the following components by weight: 55-65 parts of corn, 1-10 parts of corn lees, 10-15 parts of wheat, 12-18 parts of soybean meal, 1-3 parts of coconut oil, 0.5-1.5 parts of fish oil, 3-5 parts of plant extract, 0.1-0.5 parts of monolaurin, 1-2 parts of functional additives, 0.5-1 parts of premix, and 1-1.5 parts of amino acids.

[0006] Specifically, the components in this feed formula work synergistically to promote the intestinal health and growth performance of pigs. Corn and wheat, as the main energy sources, provide carbohydrates and part of the protein required for feed; corn alcohol grains are rich in high-quality protein and soluble fiber, which can promote intestinal fermentation; soybean meal provides high-quality plant protein to meet the essential amino acid needs of pigs. Coconut oil is rich in medium-chain fatty acids, especially lauric acid, which synergizes with the added monolaurin; fish oil provides essential polyunsaturated fatty acids, improves immune function and promotes intestinal anti-inflammation. Monolaurin not only has a broad-spectrum antibacterial effect, but also optimizes the structure of intestinal flora, reduces the colonization of pathogenic bacteria, and acts as an emulsifier to improve the absorption and utilization rate of fat-soluble nutrients. The modified montmorillonite-sodium butyrate complex is prepared by a special process to achieve the sustained release of sodium butyrate in the intestine, directly provide energy for intestinal epithelial cells, and repair damaged intestinal mucosa. At the same time, the modified montmorillonite has the ability to selectively adsorb toxins and harmful substances. The premix provides essential vitamins and trace minerals to maintain normal physiological metabolism; amino acids specifically regulate intestinal immune function and barrier integrity. This feed has established a technical system for comprehensive regulation of pig intestinal health through multiple mechanisms of action, achieving comprehensive regulation of the intestine: monolaurin and functional additives work synergistically to regulate the balance of flora and inhibit the growth of harmful bacteria on the one hand, and provide nutrients required by intestinal epithelial cells and repair the intestinal mucosal barrier on the other hand; plant extracts and amino acids provide antioxidant, anti-inflammatory and immune regulation guarantees, and strengthen the intestinal defense function; basic nutritional components ensure the energy and protein levels of the feed and optimize the nutrient absorption environment. This multi-level, multi-target synergistic effect not only significantly improves the intestinal health of pigs, reduces the incidence of intestinal inflammation and diarrhea, but also improves feed conversion efficiency and growth performance, and reduces breeding costs. At the same time, due to the optimization of intestinal function and nutrient absorption, it also reduces the emission of harmful substances such as ammonia nitrogen. It has significant economic and environmental value and provides innovative solutions for pig feed technology in the context of antibiotic substitution.

[0007] On the basis of the above technical scheme, preferably, the plant extract includes Acanthopanax senticosus root extract, Hippophae rhamnoides leaf polyphenols and Eucommia ulmoides male flower extract, and the mass ratio of Acanthopanax senticosus root extract, Hippophae rhamnoides leaf polyphenols and Eucommia ulmoides male flower extract is 2:1:1.5-2.

[0008] Specifically, the Acanthopanax senticosus root extract contains bioactive ingredients such as eleutheroside and betulinol, which can enhance the resistance of pigs to environmental stress, and at the same time improve intestinal microcirculation and oxygen supply to epithelial cells, providing a basic guarantee for the normal function of the intestinal barrier; Seabuckthorn leaf polyphenols are rich in strong antioxidant substances such as flavonoids and proanthocyanidins, which can maintain intestinal immune balance; Eucommia ulmoides male flower extract contains unique active substances such as Eucommia ulmoides polysaccharide and chlorogenic acid, which can regulate the intestinal flora structure and promote the production of short-chain fatty acids. At the same time, the plant extract can form a "trinity" intestinal protection network with monolaurin and modified montmorillonite-sodium butyrate complex in the feed. Monolaurin regulates the microbial balance, the modified montmorillonite-sodium butyrate complex repairs the barrier structure and function, and the specific ratio of the plant extract provides comprehensive protection from the aspects of regulating immune response and antioxidant defense. The three work together to achieve all-round and multi-level regulation of pig intestinal health, fundamentally solving the technical bottleneck that it is difficult for a single additive to comprehensively improve intestinal health.

[0009] Based on the above technical solutions, preferably, the amino acids include tryptophan, lysine and arginine, and the mass ratio of tryptophan, lysine and arginine is 1-1.5∶1∶2.

[0010] Based on the above technical solutions, preferably, the premix includes two or more of vitamin A, vitamin C, vitamin D and vitamin E. More preferably, based on the weight of the premix, vitamin A is 9000-10000 IU / kg, vitamin C is 1-2 mg / kg, vitamin D is 600-750 IU / kg, and vitamin E is 60-80 IU / kg.

[0011] Based on the above technical solutions, preferably, the functional additive is a modified montmorillonite-sodium butyrate complex.

[0012] Based on the above technical solutions, preferably, the preparation method of the modified montmorillonite-sodium butyrate complex is as follows:

[0013] Disperse sodium-based montmorillonite in an acidic solution of 2-3 mol / L, and stir at a constant temperature of 70-80 °C for 1-2 h to obtain modified montmorillonite; disperse the modified montmorillonite and sodium butyrate in deionized water, and stir at a constant temperature of 40-50 °C for 4-6 h to obtain a modified montmorillonite-sodium butyrate complex.

[0014] Based on the above technical solutions, preferably, the solid-liquid ratio of the sodium-based montmorillonite to the acidic solution is 1:9-10; the acidic solution is hydrochloric acid or nitric acid; the mass ratio of the modified montmorillonite to the sodium butyrate is 2.5-3.5:1.

[0015] First, sodium-based montmorillonite is acidified to increase its specific surface area and porosity, enhancing its loading capacity and slow-release characteristics for sodium butyrate. The modified montmorillonite and sodium butyrate are heated and stirred to promote the uniform distribution of sodium butyrate between the layers and on the surface of the modified montmorillonite. On the one hand, the modified montmorillonite partially has the ability to selectively adsorb intestinal toxins, heavy metal ions, and harmful bacterial toxins, removing harmful substances from the intestine. More importantly, the complex can achieve the precise slow release of sodium butyrate in an environment with changing intestinal pH gradients, enabling butyric acid to directly act on colon epithelial cells, providing them with a preferred energy source, and effectively repairing the damaged intestinal mucosal barrier. In addition, butyric acid can also play an anti-inflammatory and immune regulatory function by regulating G protein-coupled receptor 43 (GPR43) and histone deacetylation. Compared with the traditional direct addition of sodium butyrate, this complex not only solves the problem of the bad smell of butyric acid and improves the palatability of the feed, but more crucially, greatly enhances the stability and bioavailability of sodium butyrate.

[0016] Based on the above technical solution, preferably, the feed further comprises 0.5 - 1 part of probiotic preparation.

[0017] Based on the above technical solution, preferably, the probiotic preparation includes one or more of Lactobacillus acidophilus, Bifidobacterium longum, and Lactobacillus plantarum, and the carrier is maltodextrin; more preferably, in the probiotic preparation, Lactobacillus acidophilus ≥ 5.0×10 9 CFU / g; Bifidobacterium longum ≥ 1.0×10 8 CFU / g; Lactobacillus plantarum ≥ 2.0×10 7 CFU / g.

[0018] The present invention provides a method for preparing a feed containing monolaurin, comprising the following steps:

[0019] S1: Put corn, corn distillers grains, wheat, and soybean meal into a crusher according to a set ratio, and pass through a 30-mesh sieve;

[0020] S2: Put the crushed raw materials into a mixer, and add coconut oil, fish oil, plant extract, monolaurin, functional additive, premix, amino acid, and probiotic preparation according to a set ratio, and mix for 10 - 15 min;

[0021] S3: Put the mixed raw materials into the conditioner of a granulator, and the granulation temperature is 70 - 78 °C;

[0022] S4: After cooling and air-drying the granulated materials, sieve them to remove small particles to obtain the finished feed.

[0023] The feed containing monolaurin and its preparation method of the present invention have the following beneficial effects compared with the prior art:

[0024] (1) A multi-level intestinal health regulation system was constructed by combining glycerol monolaurate, modified montmorillonite-sodium butyrate complex, plant extracts, and amino acids in the basal diet. This feed system showed significant effects of reducing the diarrhea incidence rate and increasing the daily weight gain in pig feeding experiments. Its mechanism of action is as follows: Glycerol monolaurate regulates the balance of intestinal flora, selectively inhibits harmful bacteria, and promotes the proliferation of beneficial bacteria; the plant extract complex provides antioxidant and anti-inflammatory protection; the modified montmorillonite-sodium butyrate complex repairs the tight junction structure of the intestinal epithelium; specific ratios of amino acids optimize the immune response. This multi-target synergistic action mode solves the technical problem of limited effects of single additives and establishes a systematic intestinal health management plan, which is significantly superior to the existing microecological feed technology;

[0025] (2) In the plant extracts of the present invention: Eleutherococcus senticosus root extract provides basic anti-stress ability and the effect of improving microcirculation, Seabuckthorn leaf polyphenols play a precise antioxidant role and anti-inflammatory effect, and Eucommia ulmoides male flower extract promotes the development of intestinal villi and the regulation of the flora structure. This specific ratio enables the three extracts to form a complementary protection network, which can simultaneously regulate intestinal oxidative stress, inflammatory response, and barrier function, solving the technical defect of single and limited targets of single plant extracts and providing an all-round protection barrier for intestinal health.

[0026] (3) The modified montmorillonite-sodium butyrate complex realizes the precise delivery and sustained release of butyric acid in the intestine. This complex has high stability in the gastric environment and can achieve continuous sustained release in the intestinal pH environment, ensuring that butyric acid exerts the best effect in the colon. Its mechanism of action includes: providing a preferential energy source for intestinal epithelial cells, promoting intestinal epithelial repair and the expression of tight junction proteins; playing anti-inflammatory and immunomodulatory functions through specific receptors and epigenetic regulation; at the same time, modified montmorillonite has the ability to selectively adsorb intestinal toxins. This dual functionality solves the technical problems of poor stability and uncontrollable release of traditional sodium butyrate products, and ensures the uniformity and batch stability of the product through precise control of the solid-liquid ratio and mass ratio. Detailed implementation mode

[0027] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative work fall within the scope of protection of the present invention.

[0028] Example 1

[0029] This embodiment provides a feed containing monolaurin and its preparation method. By weight, it includes the following components: 60 kg of corn, 5 kg of corn distillers grains, 13 kg of wheat, 15 kg of soybean meal, 2 kg of coconut oil, 1.0 kg of fish oil, 4 kg of plant extract, 0.3 kg of monolaurin, 1.5 kg of modified montmorillonite-sodium butyrate complex, 0.8 kg of premix, 1.3 kg of amino acids, and 0.8 kg of probiotic preparation. Among them, the mass ratio of acanthopanax root extract, seabuckthorn leaf polyphenol, and eucommia male flower extract in the plant extract is 2:1:1.8; the amino acids include tryptophan, lysine, and arginine, and the mass ratio of tryptophan, lysine, and arginine is 1.3:1:2; the premix includes 9500 IU / kg of vitamin A, 1.5 mg / kg of vitamin C, 700 IU / kg of vitamin D, and 70 IU / kg of vitamin E; in the probiotic preparation, Lactobacillus acidophilus is 6.0×10 9 CFU / g; Bifidobacterium longum is 2.0×10 8 CFU / g; Lactobacillus plantarum is 3.0×10 7 CFU / g, and the carrier is maltodextrin.

[0030] Among them, the preparation method of the modified montmorillonite-sodium butyrate complex is as follows:

[0031] Disperse 1 kg of sodium-based montmorillonite in 9.5 L of 2.5 mol / L hydrochloric acid solution, stir at a constant temperature of 75 °C for 1.5 h, with a stirring speed of 300 r / min, then centrifuge and separate, collect the solid, wash it 3 times with deionized water until the pH reaches 5.0 - 6.0, and dry it at 60 °C for 12 h to obtain modified montmorillonite; disperse 3.0 kg of modified montmorillonite and 1 kg of sodium butyrate in 10 L of deionized water, stir at a constant temperature of 45 °C for 5 h, with a stirring speed of 250 r / min, then filter and collect the solid, dry it at 70 °C for 24 h, and grind it through a 100-mesh sieve to obtain the modified montmorillonite-sodium butyrate complex.

[0032] The preparation method of the feed is as follows:

[0033] S1. Put corn, corn distillers grains, wheat, and soybean meal into a crusher according to the set ratio, and pass through a 30-mesh sieve;

[0034] S2. Put the crushed raw materials into a mixer, and then add the premix, amino acids, modified montmorillonite-sodium butyrate complex, probiotic agent, and plant extract respectively according to the set ratio, and continue to mix for 5 min; then pre-mix coconut oil and fish oil with monolaurin evenly at 60 °C to form an oil mixture, and spray it evenly into the mixer through a spray system, and mix for 5 min;

[0035] S3. Put the mixed raw materials into a granulator, and the granulation temperature is 75 °C;

[0036] S4. After cooling, air-drying, and sieving the granulated material to remove small particles, the finished feed is obtained.

[0037] Example 2

[0038] This example provides a feed containing monoglyceride laurate and its preparation method. By weight, it includes the following components: 55 kg of corn, 1 kg of corn distillers grains, 10 kg of wheat, 12 kg of soybean meal, 1 kg of coconut oil, 0.5 kg of fish oil, 3 kg of plant extract, 0.1 kg of monoglyceride laurate, 1 kg of modified montmorillonite-sodium butyrate complex, 0.5 kg of premix, 1 kg of amino acids, and 0.5 kg of probiotic preparation. Among them, the mass ratio of acanthopanax root extract, seabuckthorn leaf polyphenols, and eucommia male flower extract is 2:1:1.5; the amino acids include tryptophan, lysine, and arginine, and the mass ratio of tryptophan, lysine, and arginine is 1:1:2; the premix includes 9000 IU / kg of vitamin A and 1 mg / kg of vitamin C; in the probiotic preparation, Lactobacillus acidophilus is 5.0×10 9 CFU / g; Bifidobacterium longum is 1.0×10 8 CFU / g, and the carrier is maltodextrin.

[0039] Among them, the preparation method of the modified montmorillonite-sodium butyrate complex is as follows:

[0040] Disperse 1 kg of sodium-based montmorillonite in 9 L of 2 mol / L hydrochloric acid solution, stir at a constant temperature of 70°C for 2 h, with a stirring speed of 300 r / min, then centrifuge and separate, collect the solid, wash it with deionized water 3 times until the pH reaches 5.0 - 6.0, and dry it at 60°C for 12 h to obtain modified montmorillonite; disperse 2.5 kg of modified montmorillonite and 1 kg of sodium butyrate in 10 L of deionized water, stir at a constant temperature of 40°C for 6 h, with a stirring speed of 250 r / min, then filter and collect the solid, dry it at 70°C for 24 h, and grind it through a 100-mesh sieve to obtain the modified montmorillonite-sodium butyrate complex.

[0041] The preparation method of the feed is as follows:

[0042] S1. Put corn, corn distillers grains, wheat, and soybean meal into a crusher according to the set ratio and pass through a 30-mesh sieve;

[0043] S2. Put the crushed raw materials into a mixer, and then add the premix, amino acids, modified montmorillonite-sodium butyrate complex, probiotic agent, and plant extract respectively according to the set ratio, and continue to mix for 8 min; then pre-mix coconut oil and fish oil with monoglyceride laurate at 60°C to form an oil mixture, and evenly spray it into the mixer through a spray system and mix for 5 min;

[0044] S3. Feed the mixed raw materials into a granulator, with the granulation temperature being 70°C;

[0045] S4. After cooling and air-drying the granulated materials, sieve them to remove small particles to obtain the finished feed.

[0046] Example 3

[0047] This example provides a feed containing monolaurin and its preparation method. By weight, it includes the following components: 65 kg of corn, 10 kg of corn distillers grains, 15 kg of wheat, 18 kg of soybean meal, 3 kg of coconut oil, 1.5 kg of fish oil, 5 kg of plant extract, 0.5 kg of monolaurin, 2 kg of modified montmorillonite-sodium butyrate complex, 1 kg of premix, 1.5 kg of amino acids, and 1 kg of probiotic preparation, where the mass ratio of acanthopanax root extract, seabuckthorn leaf polyphenol, and eucommia male flower extract is 2:1:2; the amino acids include tryptophan, lysine, and arginine, and the mass ratio of tryptophan, lysine, and arginine is 1.5:1:2; the premix includes 750 IU / kg of vitamin D and 80 IU / kg of vitamin E; in the probiotic preparation, Lactobacillus acidophilus is 8.0×10 9 CFU / g, and the carrier is maltodextrin.

[0048] Among them, the preparation method of the modified montmorillonite-sodium butyrate complex is as follows:

[0049] Disperse 1 kg of sodium-based montmorillonite in 10 L of 3 mol / L hydrochloric acid solution, stir at a constant temperature of 80°C for 1 h, with the stirring speed being 300 r / min, then centrifuge and separate, collect the solid, wash it 3 times with deionized water until the pH reaches 5.0 - 6.0, and dry it at 60°C for 12 h to obtain modified montmorillonite; disperse 3.5 kg of modified montmorillonite and 1 kg of sodium butyrate in 10 L of deionized water, stir at a constant temperature of 50°C for 4 h, with the stirring speed being 250 r / min, then filter and collect the solid, dry it at 70°C for 24 h, and grind it through a 100-mesh sieve to obtain the modified montmorillonite-sodium butyrate complex.

[0050] The preparation method of the feed is as follows:

[0051] S1. Feed corn, corn distillers grains, wheat, and soybean meal into a crusher according to the set ratio and sieve through a 30-mesh sieve;

[0052] S2. Feed the crushed raw materials into a mixer, then add the premix, amino acids, modified montmorillonite-sodium butyrate complex, probiotic agent, and plant extract respectively according to the set ratio, and continue to mix for 10 min; pre-mix coconut oil and fish oil with monolaurin evenly at 60°C to form an oil mixture, and spray it evenly into the mixer through a spraying system and mix for 5 min;

[0053] S3. Feed the mixed raw materials into a granulator, with the granulation temperature being 78 °C;

[0054] S4. After cooling and air-drying the granulated materials, sieve them to remove small particles to obtain the finished feed.

[0055] Comparative Example 1

[0056] This comparative example provides a feed containing monolaurin and its preparation method. The feed composition and preparation method are the same as those in Example 1, except that: the mass ratio of eleutherococcus senticosus root extract to seabuckthorn leaf polyphenols in the plant extract is 2:1.

[0057] Comparative Example 2

[0058] This comparative example provides a feed containing monolaurin and its preparation method. The feed composition and preparation method are the same as those in Example 1, except that: the mass ratio of seabuckthorn leaf polyphenols to eucommia ulmoides male flower extract in the plant extract is 1:1.8.

[0059] Comparative Example 3

[0060] This comparative example provides a feed containing monolaurin and its preparation method. The feed composition and preparation method are the same as those in Example 1, except that: the mass ratio of seabuckthorn leaf polyphenols to eucommia ulmoides male flower extract in the plant extract is 2:1.8.

[0061] Comparative Example 4

[0062] This comparative example provides a feed containing monolaurin and its preparation method. The feed composition and preparation method are the same as those in Example 1, except that: the functional additive is a montmorillonite-sodium butyrate complex, and the preparation method of the montmorillonite-sodium butyrate complex is as follows:

[0063] Disperse 3.0 kg of montmorillonite and 1 kg of sodium butyrate in 10 L of deionized water, stir at a constant temperature of 45 °C for 5 h, with the stirring speed being 250 r / min, then filter to collect the solid, dry at 70 °C for 24 h, and grind through a 100-mesh sieve to obtain the modified montmorillonite-sodium butyrate complex.

[0064] Comparative Example 5

[0065] This comparative example provides a feed and its preparation method. The feed composition and preparation method are the same as those in Example 1, except that it does not contain monolaurin.

[0066] Performance Testing

[0067] The feed of the present invention was applied in the experiment on healthy piglets with similar weights. The piglets were divided into experimental groups 1 - 3 and control groups 1 - 5, with 20 piglets in each group. Among them, experimental groups 1 - 3 were fed with the feeds prepared in Examples 1 - 3 respectively, and the control groups were fed with the feeds prepared from the traditional pig feed of Comparative Examples 1 - 5 respectively. The piglets in each group were fed 3 times a day, the test period was 2 months, and they had free access to food during the test period. At the same time, sufficient drinking water was provided. Other daily management measures were carried out according to the conventional management of the farm, and immunization and deworming were carried out regularly. After 2 months, the daily weight gain, daily feed intake, and the number of Escherichia coli in the feces of the piglets in each group were detected. At the same time, the incidence of disease in the pigs in experimental groups 1 - 3 and control groups 1 - 5 during the test period was counted, and the diarrhea rate and mortality rate of the pigs in each group were calculated. The performance test results are shown in Table 1.

[0068] Table 1 Performance Test

[0069]

[0070]

[0071] As can be seen from Table 1, the technical solution of the present invention significantly improves the growth performance and intestinal health of piglets through the synergistic effect of multiple components. The daily weight gain of the piglets in the experimental group reached 465 ± 18 g, the diarrhea rate was only 5%, and there was no death. In contrast, in control groups 1 - 5, due to the adjustment of ingredients or processes (such as imbalance in the proportion of plant extracts, unmodified montmorillonite, or lack of glycerol monolaurate), the daily weight gain decreased (the lowest was 385 ± 15 g), the diarrhea rate increased (the highest reached 25%), and even the mortality rate increased. Among them, in control group 5 (without glycerol monolaurate), the number of Escherichia coli increased sharply, further confirming the key role of this ingredient in regulating the intestinal flora.

[0072] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. A feed containing monoglyceride laurate, characterized in that, By weight parts, it includes the following components: 55 - 65 parts of corn, 1 - 10 parts of corn distillers grains, 10 - 15 parts of wheat, 12 - 18 parts of soybean meal, 1 - 3 parts of coconut oil, 0.5 - 1.5 parts of fish oil, 3 - 5 parts of plant extract, 0.1 - 0.5 parts of monoglyceride laurate, 1 - 2 parts of functional additive, 0.5 - 1 part of premix, and 1 - 1.5 parts of amino acid.

2. The feed containing glycerol monolaurate according to claim 1, characterized in that: The plant extract includes acanthopanax root extract, seabuckthorn leaf polyphenol, and eucommia male flower extract, and the mass ratio of acanthopanax root extract, seabuckthorn leaf polyphenol, and eucommia male flower extract is 2:1:1.5 - 2.

3. The feed containing glycerol monolaurate according to claim 1, characterized in that: The amino acid includes tryptophan, lysine, and arginine, and the mass ratio of tryptophan, lysine, and arginine is 1 - 1.5:1:

2.

4. A feed containing glycerol monolaurate as described in claim 1, characterized in that: The premix includes two or more of vitamin A, vitamin C, vitamin D, and vitamin E.

5. A feed containing glycerol monolaurate as described in claim 1, characterized in that: The functional additive is a modified montmorillonite - sodium butyrate complex.

6. A feed containing monoglyceryl laurate according to claim 5, characterized in that: The preparation method of the modified montmorillonite - sodium butyrate complex is as follows: Disperse sodium - based montmorillonite in an acidic solution of 2 - 3 mol / L, and stir at a constant temperature of 70 - 80 °C for 1 - 2 h to obtain modified montmorillonite; Disperse the modified montmorillonite and sodium butyrate in deionized water, and stir at a constant temperature of 40 - 50 °C for 4 - 6 h to obtain the modified montmorillonite - sodium butyrate complex.

7. A feed containing glycerol monolaurate according to claim 6, characterized in that: The solid - liquid ratio of the sodium - based montmorillonite to the acidic solution is 1:9 - 10; the acidic solution is hydrochloric acid or nitric acid; the mass ratio of the modified montmorillonite to sodium butyrate is 2.5 - 3.5:

1.

8. A feed containing glycerol monolaurate according to claim 1, characterized in that: The feed also includes 0.5 - 1 part of probiotic preparation.

9. A feed containing glycerol monolaurate according to claim 8, characterized in that: The probiotic preparation includes one or more of lactobacillus acidophilus, bifidobacterium longum, and lactobacillus plantarum, and the carrier is maltodextrin.

10. The preparation method of a feed containing glycerol monolaurate according to any one of claims 1-9, characterized in that: It includes the following steps: S1. Put corn, corn distillers grains, wheat, and soybean meal into a crusher according to the set ratio, and pass through a 30 - mesh sieve; S2. Put the crushed raw materials into a mixer, and add coconut oil, fish oil, plant extract, monoglyceride laurate, functional additive, premix, and amino acid according to the set ratio, and mix for 10 - 15 min; S3. Put the mixed raw materials into the conditioner of a granulator, and the granulation temperature is 70 - 78 °C; S4. After cooling and air - drying the granulated materials, pass through a sieve to remove small particles to obtain the finished feed.

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