Medicinal and edible composition and application thereof in enhancing alcohol metabolism

Abstract

The invention discloses a medicinal and edible composition which is characterized by comprising the following components in parts by weight: 15-20 parts of a hovenia dulcis thunb extract, 10-15 parts of a turmeric extract, 8-12 parts of a schisandra chinensis extract, 5-8 parts of a globe artichoke extract, 6-10 parts of a ganoderma lucidum spore powder extract and 0.5-1 part of a silibinin extract. According to the composite composition prepared by the invention, ethanol metabolism key enzyme (ADH / ALDH) is activated by multiple targets, acetaldehyde removal is accelerated, and a hepatocyte repairing function is supplemented, so that alcohol metabolism after drinking is accelerated, and damage of alcohol to a human body is reduced.

Description

Technical Field

[0001] The invention relates to the technical field of functional food preparation, and in particular to a composite composition with a medicine-food homologous component as a core and an application thereof in enhancing alcohol metabolism. Background Art

[0002] Existing alcohol-relief products mostly rely on B vitamins (such as metadoxine) to relieve symptoms in the short term, but may increase the burden on the liver and kidneys. There are a wide variety of alcohol-relief products on the market, but there are still certain limitations. CN101874857B discloses a liver-protecting liquor and its production process for resisting alcoholic liver damage. It uses kudzu root, wolfberry, hawthorn, papaya, etc. as main raw materials, and protects the liver through flavonoids to exert antioxidant effects, but lacks direct activation ingredients for ADH / ALDH, and does not specifically solve the problem of acetaldehyde accumulation. CN119488152A discloses a composition and its preparation method and application. Although it proposes a combination of kudzu root and Hovenia dulcis, it does not introduce Nrf2 pathway regulatory ingredients to enhance ALDH activity. CN118845987A discloses a plant carbon point alcohol-relief health care composition, which proposes to use plant carbon points to adsorb alcohol metabolites, but relies on non-medicinal and edible homologous ingredients (such as scorpion grass), and its long-term safety is questionable. CN117502650A discloses a composition, a preparation method and an application thereof, which enhances the detoxification ability by compounding artichoke extract with rattan tea, but does not involve the regulation of ADH / ALDH gene expression.

[0003] It can be determined from the above prior art that the current hangover-relieving compositions usually intervene in a single metabolic pathway, only activating ADH or adsorbing acetaldehyde, lacking multi-target synergistic effects. In addition, the integrated design of liver cell repair and anti-oxidative stress is also neglected. Summary of the invention

[0004] The main purpose of the present invention is to provide a composition that can activate key enzymes of ethanol metabolism (ADH / ALDH) through multiple targets, accelerate the removal of acetaldehyde, and assist with liver cell repair function, so as to accelerate alcohol metabolism after drinking and reduce the damage of alcohol to the human body.

[0005] A medicinal and edible composition consists of the following components in parts by weight: 15-20 parts of Hovenia dulcis fruit extract, 10-15 parts of Curcuma longa extract, 8-12 parts of Schisandra chinensis extract, 5-8 parts of Artichoke extract, 6-10 parts of Ganoderma lucidum spore powder extract and 0.5-1 part of Silybin extract.

[0006] As another object of the present invention, the present invention also provides a method for preparing the above-mentioned medicine-food composition, comprising the following steps:

[0007] (1) Mix the extracts of Hovenia dulcis, Curcuma longa, Schisandra chinensis, Cynara scolymus, Ganoderma lucidum spores and silybin;

[0008] (2) Spray-dry and granulate the mixture obtained in step (1) using water as a binder to obtain the food-medicinal homology composition.

[0009] Preferably, the process conditions for the spray-drying granulation in step (2) are: inlet air temperature 60°C - 90°C, chamber temperature 40 - 70°C, fan frequency 18 - 28 Hz, flow rate 180 - 220 mL / min, atomization pressure 0.2 - 0.5 MPa.

[0010] As another object of the present invention, the present invention also provides the application of the above composition or the food-medicinal homology composition prepared by the above preparation method in the preparation of products for enhancing alcohol metabolism.

[0011] The present invention designs each component using a multi-pathway synergistic mechanism, with food-medicinal homology components as the core, and for the first time combines the activation of the Nrf2 pathway (curcumin) with the regulation of the ADH gene (Schisandra chinensis) to achieve a triple mechanism of "accelerated metabolism - enhanced detoxification - liver repair". The prepared composition can be used in products that can accelerate alcohol metabolism after drinking and reduce alcohol damage to the human body. Description of the Drawings

[0012] Figure 1 It is a schematic diagram of the synergistic action mechanism of each component of the composition of the present invention;

[0013] Figure 2 It is a schematic diagram of the experimental results of alcohol damage in the examples and comparative examples. Detailed Embodiments

[0014] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0015] Unless otherwise defined, all technical terms and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the technical field to which the embodiments of the present invention belong. If the definitions stated in this part are contrary to or inconsistent with the definitions stated in the patents, patent applications, published patent applications and other publications incorporated herein by reference, the definitions listed in this part shall prevail over the definitions incorporated herein by reference.

[0016] Unless otherwise specified, the methods used in the following examples are conventional methods. The materials, reagents, and instruments used, unless otherwise specified, are conventional materials, reagents, and instruments in this field, and those skilled in the art can obtain them through commercial channels. When an equivalent, concentration, or other value or parameter is expressed as a range, a preferred range, or a range defined by a series of upper preferred values and lower preferred values, it should be understood that all ranges formed by any pairing of any range upper limit or preferred value with any range lower limit or preferred value are specifically disclosed, regardless of whether the range is separately disclosed. For example, when the range "1 to 5" is disclosed, the described range should be interpreted as including the ranges "1 to 4", "1 to 3", "1 to 2", "1 to 2 and 4 to 5", "1 to 3 and 5", etc. When a numerical range is described in this application specification and claims, unless otherwise stated, this range is intended to include its end values and all integers and fractions within this range. In the specification and claims of this application, range limitations can be combined and / or interchanged, and if not otherwise stated, these ranges include all sub-ranges contained therein.

[0017] The synergistic mechanism of each component of the present invention is as Figure 1 shown. Among them, Hovenia dulcis cooperates with turmeric to play a core role in accelerating alcohol metabolism. Hovenia saponin in Hovenia dulcis reduces the blood acetaldehyde concentration by accelerating the binding of acetaldehyde-glutathione. Curcumin in turmeric activates the Nrf2 / ARE pathway and increases the expression level of ALDH. Schisandra chinensis and artichoke extract play a role in strengthening enzyme activity. Lignans in Schisandra chinensis up-regulate the expression of ADH mRNA, and Cynarin in artichoke extract promotes bile secretion and accelerates ethanol decomposition. Ganoderma lucidum spore powder and silybin play a role in liver protection. β-glucan in Ganoderma lucidum spore powder inhibits hepatocyte apoptosis, and silybin extract reduces the ALT / AST level.

[0018] In the specific embodiments of the present invention, the preparation methods of each component extract are carried out according to the following methods.

[0019] Extraction method of Hovenia dulcis:

[0020] a. Pretreatment: Shelling and pulverizing: After shelling Hovenia dulcis, it is pulverized to a particle size ≤ 0.5 mm. Water infiltration: The material-liquid ratio is 1:10 (water), and it is soaked for 30 minutes.

[0021] b. Enzymolysis-ultrasonic combined extraction: Enzymolysis conditions: Enzyme type: Cellulase + pectinase (mixed at 3:1); Enzyme addition amount: 2.5% (w / w raw material); Temperature: 50 °C, pH 4.5 (citric acid-phosphate buffer); Time: 2 hours.

[0022] c. Ultrasonic enhancement: Power: 500 W, frequency 40 kHz; solid-liquid ratio: 1:20 (water), temperature 55°C ± 2°C, time 40 minutes.

[0023] d. Separation and purification: Centrifugation: 4000 rpm, 15 minutes, take the supernatant.

[0024] Purification with macroporous resin: Dynamic adsorption with AB-8 resin (flow rate 2 BV / h), elution with 85% ethanol.

[0025] Concentration and drying: Vacuum concentration to a solid content ≥ 30%, spray drying (inlet air temperature 160°C, outlet air temperature 80°C).

[0026] Extraction method of Curcuma longa:

[0027] a. Pretreatment: Drying and pulverization: Cut the rhizomes of Curcuma longa into slices, dry at 50°C at low temperature and then pulverize through a 40-mesh sieve.

[0028] Supercritical CO2 degreasing: Pressure 10 MPa, temperature 40°C, time 2 hours (to remove oil interference).

[0029] b. Supercritical CO2 extraction:

[0030] Pressure 35 MPa, temperature 40°C, CO2 flow rate 30 L / h, entrainer 95% ethanol (1 mL / g raw material).

[0031] Time 180 minutes, first separation kettle: Separation conditions 60°C / 7 MPa.

[0032] c. Post-treatment:

[0033] The extract is concentrated under reduced pressure (-0.08 MPa, 50°C) to a solid content ≥ 30%, and then spray dried (inlet air temperature 160°C, outlet air temperature 80°C) to obtain a powder.

[0034] Extraction method of Schisandra chinensis:

[0035] a. Pretreatment: Drying and pulverization: Dry the fruits of Schisandra chinensis and then pulverize through a 60-mesh sieve.

[0036] Premixing with buffer solution: Acetic acid-sodium acetate buffer solution with pH 5.0, solid-liquid ratio 1:15.

[0037] b. Composite enzymatic hydrolysis-ultrasonic extraction: Enzymatic hydrolysis conditions: Enzyme type: Cellulase + β-glucanase (mixed at 2:1). Enzyme addition amount: 3.0% (w / w raw material), temperature 55°C, time 2.5 hours. Ultrasonic assistance: Power 600 W, solid-liquid ratio 1:25 (70% ethanol), temperature 60°C ± 2°C, time 50 minutes.

[0038] c. Purification: Centrifugation at 4000 rpm for 15 minutes, and take the supernatant.

[0039] Silica gel column chromatography: The eluent is chloroform - methanol (gradient from 9:1 to 7:3).

[0040] Concentration and drying: Rotary evaporation (50 °C, -0.08 MPa), followed by vacuum drying.

[0041] Extraction method of artichoke (Cynara scolymus):

[0042] a. Pretreatment: Leaf drying: Dry artichoke leaves at 50 °C until the moisture content ≤ 8%. Crushing and sieving: Pass through a 40 - mesh sieve.

[0043] b. Ethanol reflux extraction: The ratio of material to liquid is 1:15 (70% ethanol), temperature 80 °C, time 3 hours. Repeat extraction 2 times and combine the extracts.

[0044] c. Purification: Centrifugation at 3000 rpm for 10 minutes.

[0045] Membrane filtration: Remove impurities with a 0.45 - μm microporous membrane.

[0046] Concentration and drying: Concentrate under reduced pressure to an extract, and then spray - dry (inlet air temperature 150 °C).

[0047] Extraction method of Ganoderma lucidum spore powder:

[0048] a. Wall - breaking treatment: Ultrafine grinding: Grind by low - temperature air - flow to a particle size ≤ 5 μm.

[0049] Enzyme - assisted wall - breaking: Add cellulase (1.5% w / w), at 45 °C, pH 5.0, and continue to treat for 2 hours.

[0050] b. Polysaccharide extraction: Hot - water extraction: The ratio of material to liquid is 1:20, temperature 90 °C, time 4 hours. Centrifugation at 4000 rpm for 20 minutes, and take the supernatant.

[0051] c. Triterpenoid extraction: Ethanol reflux: The ratio of material to liquid is 1:15 (95% ethanol), temperature 75 °C, time 2 hours.

[0052] d. Composite drying: Spray - drying: Inlet air temperature 160 °C, outlet air temperature 80 °C, add maltodextrin (10% w / w) to prevent moisture absorption.

[0053] Extraction method of silybin (Silybum marianum):

[0054] a. Seed pretreatment: Degreasing: Extraction with n-hexane (material-liquid ratio 1:10), ultrasonic treatment at 40°C for 30 minutes to remove grease.

[0055] b. Acid hydrolysis extraction: Solvent: 0.5% hydrochloric acid - ethanol (70%), material-liquid ratio 1:15. Temperature: 60°C, time: 3 hours.

[0056] c. Purification: Adjust pH to neutral: Adjust to pH 7.0 with NaOH solution.

[0057] Column chromatography: Adsorption with polyamide resin, elution with 70% ethanol.

[0058] Crystallization: Static at low temperature of 4°C for 24 hours to precipitate crystals.

[0059] d. Drying: Vacuum drying: 40°C, -0.08 MPa, time: 12 hours.

[0060] The component ratios of each example and comparative example are in parts by weight and are shown in the following table:

[0061] Table 1 Component ratios of examples and comparative examples

[0062]

[0063] In Comparative Example 1, except for not adding Hovenia dulcis extract, the contents of other components are the same as those in Example 1. In Comparative Example 2, except for not adding Curcuma longa extract, the contents of other components are the same as those in Example 2. In Comparative Example 3, except for not adding Cynara scolymus extract, the contents of other components are the same as those in Example 3.

[0064] Experimental Example 1 Alcohol-induced AML12 cell injury experiment

[0065] Seed AML12 cells in a 96-well plate and administer drugs after overnight adherence. The samples are tested in batches. Weigh and dissolve them with complete medium. The stock solution concentration is 20 mg / ml. After ultrasonic dissolution, filter through a 0.22 μm filter membrane for standby. The model is established with 2.5% absolute ethanol, which co-intervenes with the cells together with the drugs. The administration concentrations are set at 500 μg / mL, 100 μg / mL, and 50 μg / mL. After 24 hours of drug administration for modeling, add 10% CCK-8 and incubate for a certain time, then detect the absorbance value with an enzyme-labeled instrument, and calculate the cell viability with the cell viability of the blank control well as 100%.

[0066] Normalize the experimental results. The cell viability of the model group is calculated as 70%, and the results are as Figure 2As shown, the cell viability of 1 (Comparative Example 1) (100 μg / ml), 3 (Example 1) (500 μg / ml, 100 μg / ml, 50 μg / ml), 4 (Example 2) (500 μg / ml, 100 μg / ml, 50 μg / ml), 5 (Comparative Example 3) (500 μg / ml, 100 μg / ml), and 6 (Example 3) (500 μg / ml, 100 μg / ml, 50 μg / ml) was significantly higher than that of the model group, indicating that they have a protective effect on alcoholic liver injury in vitro. It can also be seen from the experimental results that compared with the corresponding comparative examples, the cell viability in the examples was significantly improved, indicating that the various raw materials in the examples can play a synergistic effect.

[0067] Experimental Example 2 Alcohol Metabolism Experiment in Mice

[0068] The test substances were prepared by dissolving the compositions of each example and comparative example in 42-degree alcohol at a ratio of 1 g / 100 ml. Male BALB / c mice were used as experimental subjects. After 16 hours of fasting without water deprivation, the test substances with a volume of 0.15 mL / 10 g.bw were administered by oral gavage at one time. Blood was collected from the inner canthus of the eye at different time points (0.5, 1 h, 2 h, 4 h, 6 h) after drinking. 50 μl of the blood was taken with a pipette and mixed with 250 μl of ultrapure water. The collected blood was centrifuged in sequence, and the samples were collected and stored. Finally, the serum ethanol content was detected by a biochemical analyzer. The samples need to be frozen and filtered through a 0.45 μm filter before the experiment. Blood was collected from the orbital venous plexus of anesthetized mice in each group at different time points, centrifuged at a speed of 4500 r / min at low temperature for 15 minutes, and the supernatant was carefully collected. If precipitation occurs during storage, it should be centrifuged again. The centrifuged supernatant was used for the detection of the biochemical serum ethanol content, and the results are shown in the following table.

[0069] Table 2 Experimental Detection Results of Experimental Example 2

[0070]

[0071] Blank control group: No extract was added; Comparative Example 1: Lack of Hovenia dulcis extract; Comparative Example 2: Lack of Curcuma longa extract; Comparative Example 3: Lack of Cynara scolymus extract; Examples 1-3: Complete formula; Significance annotation: *p < 0.05 vs blank group; **p < 0.01 vs blank group; #p < 0.05 vs corresponding missing comparative example.

[0072] The ethanol content at each point and the area under the serum ethanol concentration-time curve (AUC) in the example groups were extremely significantly lower than those in the blank control group and also significantly lower than those in the corresponding comparative examples. This indicates that the alcohol metabolism in the examples was faster than that in the blank control group and the comparative examples. The ethanol content at each point and the area under the serum ethanol concentration-time curve (AUC) in the comparative example groups were between those in the blank group and the example groups, indicating that the composition of the examples was more reasonable and had better effects.

[0073] Experimental Example 3: Experiment on the Degree of Drunkenness in Mice

[0074] The test substances were prepared by dissolving the compositions of each example and comparative example in 42-degree alcohol at a ratio of 1 g / 100 ml. After the experimental animals were purchased, they were adaptively fed for 3 - 5 days before the experiment. The gavage dose of the 42-degree liquor was determined to be 0.15 ml / 10 g. The animals were fasted for 12 - 16 h before the experiment and allowed free access to water. They were randomly grouped by body weight and marked with neutral red. Gavage was performed in sequence, and the time was recorded. The drunkenness of the animals within 4.5 h - 5.0 h after administration was observed respectively. Record the administration time, the disappearance time of the righting reflex of the mice, and the recovery time of the righting reflex; and calculate the drunken latency period and the intoxication period. Note: The disappearance time of the righting reflex: taking 30 seconds with the mouse's back down as the standard; the recovery time of the righting reflex (sobering index): taking the mouse being able to move freely, flexibly, being energetic, and having smooth fur as the standard; drunken latency period = disappearance time of the righting reflex - administration time; drunken intoxication period = recovery time of the righting reflex - disappearance time of the righting reflex; sobering time = recovery time of the righting reflex - administration time (the sobering time is a comprehensive reflection of the drunken latency period and the intoxication period).

[0075] Table 3: Experimental Test Results of Experimental Example 3

[0076] Name Weight g Incubation period min Intoxication period min Blank control group 20.54±0.69 8.10±1.20 188.45±24.38 Comparative example 1 20.26±0.73 10.50±1.85* 165.30±20.21* Comparative example 2 20.69±0.43 12.80±2.10* 152.42±18.87* Comparative example 3 20.64±0.63 11.20±1.95* 158.15±19.05* Example 1 20.61±0.84 18.50±3.21**## 120.80±15.44**## Example 2 20.44±0.35 21.00±3.88**## 105.45±14.32**## Example 3 20.39±0.55 24.30±4.54**## 85.18±12.89**##

[0077] Note: Blank control group: without adding any extracts; Comparative Example 1: lacking Hovenia dulcis Thunb. extract; Comparative Example 2: lacking Curcuma longa L. extract; Comparative Example 3: lacking Cynara scolymus L. extract; Examples 1 - 3: complete formula; Significance annotation: compared with the blank group: *p < 0.05, **p < 0.01; compared with the corresponding missing comparative example: #p < 0.05, ##p < 0.01.

[0078] Compared with the blank control group, the latency periods of the 3 examples were extremely significantly prolonged, and the intoxication periods were extremely significantly shortened, indicating that the examples could accelerate the alcohol metabolism of the experimental animals, making the experimental animals get drunk slowly and sober up quickly. Compared with the comparative examples, the latency periods of the 3 examples were significantly prolonged, and the intoxication periods were significantly shortened, indicating that the examples had better effects than the comparative examples, and also indicating that the compositions of the examples were more reasonably matched and had better effects.

[0079] The above is only the preferred embodiment of the present invention and is not intended to limit the present invention. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A medicine and food homologous composition, characterized in that, It consists of the following components in parts by weight: 15-20 parts of Hovenia dulcis extract, 10-15 parts of turmeric extract, 8-12 parts of Schisandra chinensis extract, 5-8 parts of artichoke extract, 6-10 parts of ganoderma spore powder extract, and 0.5-1 part of silybin extract.

2. A preparation method of the medicine and food homologous composition according to claim 1, characterized in that, It includes the following steps: (1) Mix the Hovenia dulcis extract, turmeric extract, Schisandra chinensis extract, artichoke extract, ganoderma spore powder extract, and silybin extract. (2) Spray-dry and granulate the mixture obtained in step (1) using water as a binder to obtain the food-drug homologous composition.

3. The method according to claim 2, wherein The process conditions for the spray-drying granulation in step (2) are as follows: inlet air temperature 60°C - 90°C, chamber temperature 40 - 70°C, fan frequency 18 - 28 Hz, flow rate 180 - 220 mL / min, atomization pressure 0.2 - 0.5 MPa.

4. Use of the composition according to claim 1 or the food-drug homologous composition prepared by the preparation method according to any one of claims 2-3 in the preparation of a product for enhancing alcohol metabolism.

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