High-density keep-alive transportation method for rotifers

Abstract

The invention relates to a rotifer high-density keep-alive transportation method. The method comprises the following steps: quantifying newly harvested rotifers, and mixing the quantified rotifers with fresh seawater in an original pool in proportion; transferring the mixed rotifers into a double-layer polyethylene plastic bag, sealing seawater ice with a sealing bag, and putting the seawater ice into the mixed live rotifers in proportion for cooling; long-acting oxygenation particles and an oxygenation synergist are put into the double-layer sealing bag for oxygenation, and then the double-layer polyethylene plastic bag is sealed after being filled with pure oxygen; the outer side of the box body is sleeved with a layer of polyethylene plastic bag, the box body is placed in a transportation box, the gap between the box body and the sealed plastic sealing bag is filled with the combination of the seawater ice bottle and the crushed seawater ice, finally, a cover is covered, and the transportation packaging box is sealed through an adhesive tape and then placed in a paper box to be sealed for transportation. The packaging sequence is a double-layer polyethylene plastic bag, a polyethylene plastic bag, a foam box and a paper box; after the rotifers are transported to the destination, the rotifers are put into a temporary rearing pond to be activated and then temporarily rearing, photosynthetic bacteria, EM bacteria and vitamin C are added into the temporary rearing pond, and the survival rate of the rotifers is increased.

Description

Technical Field

[0001] The present invention relates to the technical field of aquaculture, and in particular to a method for high-density live transportation of rotifers. Background Art

[0002] Rotifers are important initial live food organisms for aquaculture seedling rearing. Their application as live food has greatly promoted the development of the seedling production in the aquaculture industry. With the continuous development and growth of the aquaculture industry, the demand for rotifers as high-quality live food has also been increasing. Since the cultivation of rotifers in indoor cement ponds has low cultivation density (generally not exceeding 200 individuals / mL), low yield, long cycle, and requires the prior cultivation of single-celled algae, occupying a large amount of water space for seedling rearing, it cannot meet the needs of seedling production supply. Therefore, at present, rotifers in China are mainly applied by the method of artificial cultivation in outdoor earthen ponds and then transported to the seedling rearing farms. Due to the inconsistent seedling rearing times of different aquatic animals, resulting in a large span of seedling rearing time, and the need to ensure the survival rate of rotifers for subsequent nutritional enrichment, it is necessary to ensure the transportation survival rate of rotifers. However, in the current high-density transportation of rotifers, especially during long-distance transportation, due to improper transportation methods, a large number of rotifers often die during transportation; secondly, due to the large time span, long-distance transportation at higher temperatures in summer is more likely to result in a high transportation mortality rate of rotifers. These unfavorable factors have hindered the development of the aquaculture seedling rearing industry.

[0003] In the prior art, most often use oxygen filling in plastic bags and then putting them into a foam box with ice cubes for temperature reduction to transport live rotifers. However, as the transportation time prolongs, due to reasons such as lack of oxygen and unstable temperature reduction, when using the prior art for high-density transportation of rotifers, problems such as too high rotifer mortality rate and unstable temporary cultivation survival rate (generally lower than 50%) are likely to occur, which not only causes economic losses, but also causes the death of aquatic fry due to insufficient supply of initial food, having an adverse impact on the seedling rearing of aquatic animals. Summary of the Invention

[0004] To solve the above problems, the present invention provides a method for high-density live transportation of rotifers, which includes: preparation of transportation materials, collection of rotifers, quantitative mixing, cooling and oxygen increasing, packaging, activation and temporary cultivation;

[0005] The operation steps are as follows:

[0006] S1 Preparation of transportation materials: including double-layer polyethylene plastic bags, polyethylene plastic bags, polystyrene foam boxes with lids, cardboard boxes, seawater ice, long-acting oxygen-increasing granules, oxygen-increasing synergists and oxygen-filling equipment;

[0007] S2 Collection of rotifers: Select an appropriate time to collect rotifers, collect rotifers through a suspended water pump and a 200-mesh long nylon mesh bag, and temporarily store the live rotifers after filtering out the water for later use;

[0008] S3 Quantitative mixing: After quantifying the rotifers, mix the rotifers with the seawater from the original pond in a volume ratio of 1:1, and then put the mixture into a double-layer polyethylene plastic bag with dimensions of 40 cm × 60 cm and mix well;

[0009] S4 Cooling and oxygenation: Put 2000 - 2500 parts of crushed seawater ice into a plastic sealed bag, seal it, and then put it into a double-layer polyethylene plastic bag containing 5 - 10 L of rotifers to cool; put in 50 - 100 parts of long-acting oxygenation granules and 50 - 100 parts of oxygenation synergist, and then fill it with oxygen and seal it;

[0010] S5 Packaging: Pack in the order of double-layer polyethylene plastic bag - polyethylene plastic bag - foam box - cardboard box. Among them, use a combination of seawater ice bottles and crushed seawater ice to fill the gap between the box body and the sealed plastic bag at the mouth, cover the lid, seal it with tape, and then put it into a cardboard box and seal it. The sizes of the plastic box and the cardboard box are 30 L;

[0011] S6 Activation and temporary cultivation: After the rotifers arrive at the destination, remove the packaging box, take out the crushed ice in the double-layer polyethylene plastic bag, reseal it, and then put it into the temporary cultivation pond to slowly warm up. The water temperature of the temporary cultivation pond is 20 - 25 °C, and the salinity is 20 - 30%; when the temperature difference between the temperature inside the bag and the water temperature of the stocking pond does not exceed 5 °C, the rotifers can be put into the pond for activation, with a density of 1 - 2 kg / m 3 , before the rotifers enter the pond, sprinkle photosynthetic bacteria, EM bacteria and vitamin C to reduce the stress response of the rotifers and improve the survival rate.

[0012] The rotifer harvesting time in S2 is preferably during the aquaculture seedling breeding period from March to May or from September to November, preferably in the morning or afternoon, avoiding the time with higher temperature at noon.

[0013] The raw material seawater salinity of the crushed seawater ice in S4 is 20 - 32%.

[0014] The water temperature of the temporary cultivation pond in S6 is 23 - 25 °C, and the salinity is 20 - 30%.

[0015] The concentration of photosynthetic bacteria in the temporary cultivation pond in S6 is 50 - 100 μL / L, the concentration of EM bacteria is 15 - 30 μL / L, and the concentration of vitamin C is 5 - 10 mg / L.

[0016] Sprinkle photosynthetic bacteria, EM bacteria and vitamin C 1 - 12 h before the rotifers enter the pond in S6.

[0017] The long-acting oxygenation granule is the granular oxygen of Tianjin Yikang Runze Biotechnology Co., Ltd.

[0018] The preparation method of the oxygenation synergist is:

[0019] K1: By weight, add 20 - 30 parts of sodium percarbonate, 4 - 7 parts of 6,6'-diamino trehalose, 0.4 - 0.9 parts of glucose oxidase, 2 - 6 parts of epoxidized soybean oil, 2 - 4 parts of sodium bicarbonate, and 200 - 300 parts of deionized water, and stir and mix for 120 - 150 minutes at 50 - 60 °C and 250 - 350 revolutions per minute;

[0020] K2: Then add 0.5 - 2 parts of sodium dodecyl sulfate and 5 - 10 parts of diatomite, and continue to stir at 50 - 60 °C for 30 - 40 minutes; stir for 10 - 15 minutes at 300 - 500 revolutions per minute to form a mixed solution;

[0021] K3: Post - forming treatment step: Transfer the mixed solution to a mold, let it stand at room temperature for 1 - 2 hours to form; take out the formed product and dry it for 3 - 5 hours to obtain the oxygen - increasing and efficiency - enhancing agent.

[0022] Reaction mechanism

[0023] Principle for improving the slow - release oxygen effect of sodium percarbonate:

[0024] Physical encapsulation effect: The product formed by the ring - opening reaction of 6,6'-diamino trehalose and epoxidized soybean oil encapsulates sodium percarbonate. In the process of sodium percarbonate contacting water and releasing oxygen, it needs to first pass through these encapsulation structures, thereby slowing down the contact speed between sodium percarbonate and water and achieving the slow release of oxygen.

[0025] Chemical reaction regulation: Hydrogen peroxide catalyzed by glucose oxidase, and there is an adjustment of the redox balance between hydrogen peroxide and sodium percarbonate, making the decomposition rate of sodium percarbonate in the system become more gentle, thus achieving the effect of slow - release oxygen.

[0026] Technical effects

[0027] A method for high - density live transportation of rotifers according to the present invention has the following remarkable effects compared with the prior art:

[0028] 1. The present invention provides a method for high-density live transportation of rotifers, including steps such as material preparation, rotifer harvesting, mixing and quantification, cooling and oxygenation, packaging and transportation, activation and temporary cultivation, etc. The transportation materials are common commodities in the market, with low prices and can be reused, which can reduce costs; treating rotifers by cooling can reduce their physiological metabolism, and oxygen can be supplied sufficiently during long-distance transportation by means of oxygenation, long-acting oxygenation granules, oxygenation synergists, etc. to prevent rotifers from dying due to oxygen deficiency; during the packaging process, the packaging method of double-layer polyethylene plastic bags - polyethylene plastic bags - foam boxes - cardboard boxes can slow down the cooling as long as possible and ensure oxygen supply, improving the survival rate of rotifer transportation; after the rotifers arrive at the destination, they are activated by adding photosynthetic bacteria, EM bacteria, vitamin C, etc., further improving the survival rate of rotifers. Through the above series of treatment work, the survival rate of marine rotifers can be effectively improved, and the survival time can be extended to achieve high-density, long-distance, and long-time transportation of rotifers.

[0029] 2. The present invention conducts live transportation of rotifers under low temperature and sufficient oxygen conditions by cooling and oxygenation, alleviating the stress response of live transportation of rotifers, extending the transportation time, significantly reducing the transportation cost, and increasing the survival rate of rotifers during transportation.

[0030] 3. The present invention proposes a method for high-density live transportation of rotifers, which can significantly reduce the transportation mortality rate of rotifers and extend the transportation time; and during the temporary cultivation stage, the survival rate of rotifer activation is increased by adding probiotics, etc., meeting the requirements of high-density live transportation of rotifers and ensuring the effective supply of live rotifer biological bait for aquaculture animal seedling raising.

[0031] 4. Prolong the oxygenation time: By improving the slow-release oxygen effect of sodium percarbonate, during the aquaculture transportation process, it can continuously provide oxygen for the water body for a long time. Compared with the direct use of ordinary sodium percarbonate, this kind of long-acting oxygenation granule with epoxy groups can stably release oxygen for several hours or even several days, meeting the oxygen demand of aquaculture animals during transportation and reducing the death of aquaculture animals caused by oxygen deficiency.

[0032] 5. Improve transportation safety: The stable oxygen supply keeps the dissolved oxygen level in the water body within a relatively appropriate range, avoiding the stress response caused to aquaculture animals by excessive fluctuations in dissolved oxygen concentration. This helps to increase the survival rate of aquaculture animals during transportation, reduce transportation risks, and ensure the economic benefits of the aquaculture transportation industry.

[0033] 6. Optimize the water quality environment: The continuous oxygenation process helps to maintain the activity of aerobic microbial communities in the water body, promote the decomposition and transformation of organic matter in the water body, and reduce the accumulation of harmful substances such as ammonia nitrogen and nitrite. This not only improves the living environment of aquaculture animals, but also improves the quality of aquaculture animals after transportation, reducing the spread of diseases caused by water quality deterioration. Description of the Drawings

[0034] Figure 1 For harvesting rotifers;

[0035] Figure 2 For quantitative mixing;

[0036] Figure 3 For cooling, oxygen increasing and packaging;

[0037] Figure 4 For activation and temporary cultivation. Detailed Embodiments

[0038] To further illustrate the technical means and effects adopted by the present invention to achieve the predetermined invention purpose, the following will, in conjunction with the drawings and preferred embodiments, describe in detail the specific embodiments, structures, features and their effects according to the present invention as follows.

[0039] Live rotifers were transported from the Caofeidian area in Hebei to the Jinzhou area in Dalian, Liaoning from late April to mid-May. Each box contained 10 kg of rotifers, and the transportation time was 30 h. The survival rate was tested, and the test results are shown in Table 1.

[0040] Example 1

[0041] A method for high-density live transportation of rotifers, which includes: preparation of transportation materials, harvesting of rotifers, quantitative mixing, cooling and oxygen increasing, packaging, activation and temporary cultivation; the operation steps are as follows:

[0042] S1 Preparation of transportation materials: including double-layer polyethylene plastic bags, polyethylene plastic bags, polystyrene foam boxes with lids, cardboard boxes, seawater ice, long-acting oxygen increasing granules, oxygen increasing synergists and oxygen filling equipment;

[0043] S2 Harvesting of rotifers: Select an appropriate time to harvest rotifers, and harvest rotifers through a suspended water pump and a 200-mesh long nylon mesh bag. After the live rotifers are filtered and dried, they are temporarily stored for later use;

[0044] S3 Quantitative mixing: After the rotifers are quantified, they are mixed with the original pond seawater at a volume ratio of 1:1, and then put into a double-layer polyethylene plastic bag with a size of 40 cm × 60 cm and mixed evenly;

[0045] S4 Cooling and oxygen increasing: Put 2000 g of crushed seawater ice into a plastic sealed bag, seal it, and then put it into a double-layer polyethylene plastic bag containing 5 L of rotifers for cooling; put 50 g of long-acting oxygen increasing granules and 50 g of oxygen increasing synergists, and then fill it with oxygen and seal it;

[0046] S5 Packaging: Pack in the order of double-layer polyethylene plastic bag - polyethylene plastic bag - foam box - carton. Among them, use a combination of seawater ice bottles and crushed seawater ice to fill the gap between the box body and the sealed plastic bag at the opening. Close the lid and seal it with tape, and then put it into the carton and seal it. The sizes of the plastic box and the carton are 30L.

[0047] S6 Activation and temporary cultivation: After the rotifers arrive at the destination, remove the packaging box, take out the crushed ice in the double-layer polyethylene plastic bag, reseal it, and put it into the temporary cultivation pool to slowly warm up. The water temperature of the temporary cultivation pool is 20°C and the salinity is 20%. When the temperature difference between the temperature inside the bag and the temperature of the released pond water does not exceed 5°C, the rotifers can be put into the pond for activation, with a density of 1 kg / m 3 , Before the rotifers enter the pond, sprinkle photosynthetic bacteria, EM bacteria and vitamin C to reduce the stress response of the rotifers and improve the survival rate.

[0048] The rotifer collection time in S2 is preferably during the aquatic seedling breeding period from March to May or from September to November, preferably in the morning or afternoon, avoiding the time with higher temperature at noon.

[0049] The raw material seawater salinity of the crushed seawater ice in S4 is 20%.

[0050] The water temperature of the temporary cultivation pool in S6 is 23°C and the salinity is 20%.

[0051] The concentration of photosynthetic bacteria in the temporary cultivation pool in S6 is 50 μL / L, the concentration of EM bacteria is 15 μL / L, and the concentration of vitamin C is 5 mg / L.

[0052] Sprinkle photosynthetic bacteria, EM bacteria and vitamin C 1 hour before the rotifers enter the pond in S6.

[0053] The long-acting oxygen-increasing granule is the granular oxygen of Tianjin Yikang Runze Biotechnology Co., Ltd.

[0054] The preparation method of the oxygen-increasing and efficiency-enhancing agent is as follows:

[0055] K1: Mix 20 g of sodium percarbonate, 4 g of 6,6'-diamino trehalose, 0.4 g of glucose oxidase, 2 g of epoxidized soybean oil, 2 g of sodium bicarbonate, and 200 g of deionized water under the conditions of 50°C and 250 revolutions per minute, and stir and mix for 120 minutes;

[0056] K2: Then add 0.5 g of sodium dodecyl sulfate and 5 g of diatomite, and continue to stir at 50°C for 30 minutes; stir for 10 minutes under the condition of 300 revolutions per minute to form a mixed solution;

[0057] K3: Post-forming treatment step: Transfer the mixed solution to a mold, let it stand at room temperature for 1 hour to form; take out the formed product and dry it for 3 hours to obtain the oxygen-increasing and efficiency-enhancing agent.

[0058] Example 2

[0059] A method for high-density live transportation of rotifers, which includes: preparation of transportation materials, collection of rotifers, quantitative mixing, cooling and oxygenation, packaging, and activation and temporary cultivation; the operation steps are as follows:

[0060] S1 Preparation of transportation materials: including double-layer polyethylene plastic bags, polyethylene plastic bags, polystyrene foam boxes with lids, cardboard boxes, seawater ice, long-acting oxygenation granules, oxygenation enhancer, and oxygen-filling equipment;

[0061] S2 Collection of rotifers: Select an appropriate time to collect rotifers. Collect rotifers through a suspended water pump and a 200-mesh long nylon mesh bag. After filtering and draining the water from the live rotifers, store them temporarily for later use;

[0062] S3 Quantitative mixing: After quantifying the rotifers, mix the rotifers with the original pond seawater in a ratio of 1:1 by volume, and then put them into a double-layer polyethylene plastic bag with dimensions of 40 cm × 60 cm and mix well;

[0063] S4 Cooling and oxygenation: Put 2100 g of crushed seawater ice into a plastic sealed bag, seal it, and then put it into a double-layer polyethylene plastic bag containing 7 L of rotifers for cooling; put 70 g of long-acting oxygenation granules and 70 g of oxygenation enhancer, and then fill it with oxygen and seal it;

[0064] S5 Packaging: Pack in the order of double-layer polyethylene plastic bag - polyethylene plastic bag - foam box - cardboard box. Among them, use a combination of seawater ice bottles and crushed seawater ice to fill the gap between the box body and the sealed plastic bag at the mouth. Cover the lid and seal it with tape, and then put it into a cardboard box and seal it. The sizes of the plastic box and the cardboard box are 30 L;

[0065] S6 Activation and temporary cultivation: After the rotifers arrive at the destination, remove the packaging box, take out the crushed ice in the double-layer polyethylene plastic bag, re-seal it, and put it into the temporary cultivation pond to slowly warm up. The water temperature of the temporary cultivation pond is 22°C, and the salinity is 25%; when the temperature difference between the temperature inside the bag and the water temperature of the stocking pond does not exceed 5°C, the rotifers can enter the pond for activation, with a density of 1.5 kg / m 3 , before the rotifers enter the pond, sprinkle photosynthetic bacteria, EM bacteria, and vitamin C to reduce the stress response of the rotifers and improve the survival rate.

[0066] The time for collecting rotifers in S2 is preferably during the breeding period of aquatic fry from March to May or from September to November, preferably in the morning or afternoon, avoiding the time with higher temperature at noon.

[0067] The raw seawater salinity of the crushed seawater ice in S4 is 25%.

[0068] The water temperature of the temporary cultivation pond in S6 is 24°C, and the salinity is 25%.

[0069] The concentration of photosynthetic bacteria in the temporary rearing pond of S6 is 60 μL / L, the concentration of EM bacteria is 20 μL / L, and the concentration of vitamin C is 6 mg / L.

[0070] Before the rotifers of S6 enter the pond, photosynthetic bacteria, EM bacteria and vitamin C are sprinkled 5 hours in advance.

[0071] The long-acting oxygen-increasing granule is the granular oxygen of Tianjin Yikang Runze Biotechnology Co., Ltd.

[0072] The preparation method of the oxygen-increasing and efficiency-enhancing agent is as follows:

[0073] K1: Mix 23 g of sodium percarbonate, 5 g of 6,6'-diamino trehalose, 0.6 g of glucose oxidase, 3 g of epoxy soybean oil, 3 g of sodium bicarbonate and 240 g of deionized water under the conditions of 55 °C and 280 revolutions per minute for 130 minutes;

[0074] K2: Then add 1 g of sodium dodecyl sulfate and 6 g of diatomite, and continue to stir at 55 °C for 35 minutes; stir for 12 minutes under the condition of 350 revolutions per minute to form a mixed solution;

[0075] K3: Post-forming treatment step: Transfer the mixed solution to a mold and let it stand at room temperature for 1.5 hours to form; take out the formed product and dry it for 3.5 hours to obtain the oxygen-increasing and efficiency-enhancing agent.

[0076] Example 3

[0077] A method for high-density live transportation of rotifers, which includes: preparation of transportation materials, collection of rotifers, quantitative mixing, cooling and oxygenation, packaging, activation and temporary rearing; the operation steps are as follows:

[0078] S1 Preparation of transportation materials: including double-layer polyethylene plastic bags, polyethylene plastic bags, polystyrene foam boxes with lids, cardboard boxes, seawater ice, long-acting oxygen-increasing granules, oxygen-increasing and efficiency-enhancing agents and oxygen-filling equipment;

[0079] S2 Collection of rotifers: Select an appropriate time to collect rotifers, collect rotifers through a suspended water pump and a 200-mesh long nylon mesh bag, and temporarily store the live rotifers after draining the water for later use;

[0080] S3 Quantitative mixing: After the rotifers are quantified, mix the rotifers with the original pond seawater in a ratio of 1:1 by volume, and then put them into a double-layer polyethylene plastic bag with a size of 40 cm × 60 cm and mix well;

[0081] S4 Cooling and oxygenation: Put 2400 g of crushed seawater ice into a plastic seal bag and seal it, then put it into a double-layer polyethylene plastic bag containing 8 L of rotifers for cooling; put 80 g of long-acting oxygen-increasing granules and 80 g of oxygen-increasing and efficiency-enhancing agents, and then fill it with oxygen and seal it;

[0082] S5 Packaging: Pack in the order of double-layer polyethylene plastic bag - polyethylene plastic bag - foam box - cardboard box. Fill the gap between the box body and the sealed plastic bag with a combination of seawater ice bottles and crushed seawater ice. Cover the lid and seal it with tape, then put it into the cardboard box and seal it. The sizes of the plastic box and the cardboard box are 30L.

[0083] S6 Activation and temporary cultivation: After the rotifers arrive at the destination, remove the packaging box, take out the crushed ice in the double-layer polyethylene plastic bag, reseal it, and put it into the temporary cultivation pool to slowly warm up. The water temperature of the temporary cultivation pool is 24°C and the salinity is 28%. When the temperature difference between the temperature inside the bag and the water temperature of the stocking pool does not exceed 5°C, the rotifers can be put into the pool for activation, with a density of 1.5 kg / m 3 , before the rotifers enter the pool, sprinkle photosynthetic bacteria, EM bacteria and vitamin C to reduce the stress response of the rotifers and improve the survival rate.

[0084] The rotifers in S2 are preferably caught during the period of aquatic fry breeding from March to May or from September to November, preferably in the morning or afternoon, avoiding the time with higher temperature at noon.

[0085] The raw material seawater salinity of the crushed seawater ice in S4 is 30%.

[0086] The water temperature of the temporary cultivation pool in S6 is 24°C and the salinity is 28%.

[0087] The concentration of photosynthetic bacteria in the temporary cultivation pool in S6 is 90 μL / L, the concentration of EM bacteria is 25 μL / L, and the concentration of vitamin C is 8 mg / L.

[0088] Sprinkle photosynthetic bacteria, EM bacteria and vitamin C 10 h before the rotifers enter the pool in S6.

[0089] The long-acting oxygen-increasing granule is the granular oxygen of Tianjin Yikang Runze Biotechnology Co., Ltd.

[0090] The preparation method of the oxygen-increasing and efficiency-enhancing agent is as follows:

[0091] K1: Mix 28 g of sodium percarbonate, 6 g of 6,6'-diamino trehalose, 0.8 g of glucose oxidase, 5 g of epoxy soybean oil, 3 g of sodium bicarbonate, and 280 g of deionized water under the conditions of 55°C and 300 rpm for 140 minutes;

[0092] K2: Then add 1.5 g of sodium dodecyl sulfate and 8 g of diatomaceous earth, and continue to stir at 55°C for 35 minutes; stir at 450 rpm for 14 minutes to form a mixed solution;

[0093] K3: Post-forming treatment step: Transfer the mixed solution to a mold, let it stand at room temperature for 1.5 hours to form; take out the formed product and dry it for 4.5 hours to obtain the oxygen-increasing and efficiency-enhancing agent.

[0094] Example 4

[0095] A method for high-density live transportation of rotifers, which includes: preparation of transportation materials, collection of rotifers, quantitative mixing, cooling and oxygenation, packaging, and activation and temporary cultivation; the operation steps are as follows:

[0096] S1 Preparation of transportation materials: It includes double-layer polyethylene plastic bags, polyethylene plastic bags, polystyrene foam boxes with lids, cardboard boxes, seawater ice, long-acting oxygenation granules, oxygenation enhancer, and oxygenation equipment;

[0097] S2 Collection of rotifers: Select an appropriate time to collect rotifers, and collect rotifers through a suspended water pump and a 200-mesh long nylon mesh bag. After the live rotifers are drained of water, they are temporarily stored for later use;

[0098] S3 Quantitative mixing: After the rotifers are quantified, they are mixed with the original pond seawater in a ratio of 1:1 by volume, and then filled into a double-layer polyethylene plastic bag with dimensions of 40 cm × 60 cm and mixed evenly;

[0099] S4 Cooling and oxygenation: Put 2500 g of crushed seawater ice into a plastic sealed bag and seal it, then put it into a double-layer polyethylene plastic bag containing 10 L of rotifers for cooling; put 100 g of long-acting oxygenation granules and 100 g of oxygenation enhancer, and then fill it with oxygen and seal it;

[0100] S5 Packaging: Package in the order of double-layer polyethylene plastic bag - polyethylene plastic bag - foam box - cardboard box. Among them, the combination of seawater ice bottles and crushed seawater ice is used to fill the gap between the box body and the sealed plastic bag at the opening. Cover the lid and seal it with tape, and then put it into a cardboard box and seal it. The sizes of the plastic box and the cardboard box are 30 L;

[0101] S6 Activation and temporary cultivation: After the rotifers arrive at the destination, remove the packaging box, take out the crushed ice in the double-layer polyethylene plastic bag, re-seal it and put it into the temporary cultivation pond to slowly warm up. The water temperature of the temporary cultivation pond is 25 °C, and the salinity is 30%; when the temperature in the bag does not exceed 5 °C from the temperature of the stocking pond water, the rotifers can be put into the pond for activation, with a density of 2 kg / m 3 , before the rotifers enter the pond, sprinkle photosynthetic bacteria, EM bacteria, and vitamin C to reduce the stress response of the rotifers and improve the survival rate.

[0102] The time for collecting rotifers in S2 is preferably during the breeding period of aquatic fry from March to May or from September to November, preferably in the morning or afternoon, avoiding the time with higher temperature at noon.

[0103] The raw seawater salinity of the crushed seawater ice in S4 is 32%.

[0104] The water temperature of the temporary cultivation pond in S6 is 25 °C, and the salinity is 30%.

[0105] In the temporary rearing pond of S6, the concentration of photosynthetic bacteria is 100 μL / L, the concentration of EM bacteria is 30 μL / L, and the concentration of vitamin C is 10 mg / L.

[0106] Before the rotifers of S6 enter the pond, photosynthetic bacteria, EM bacteria, and vitamin C are sprinkled 12 hours in advance.

[0107] The long-acting oxygen-increasing granule is the granular oxygen of Tianjin Yikang Runze Biotechnology Co., Ltd.

[0108] The preparation method of the oxygen-increasing synergist is as follows:

[0109] K1: Mix 30 g of sodium percarbonate, 7 g of 6,6'-diamino trehalose, 0.9 g of glucose oxidase, 6 g of epoxy soybean oil, 4 g of sodium bicarbonate, and 300 g of deionized water under the conditions of 60 °C and 350 revolutions per minute, and stir and mix for 150 minutes;

[0110] K2: Then add 2 g of sodium dodecyl sulfate and 10 g of diatomite, and continue to stir at 60 °C for 40 minutes; stir at 500 revolutions per minute for 15 minutes to form a mixed solution;

[0111] K3: Post-forming treatment step: Transfer the mixed solution to a mold, and let it stand at room temperature for 2 hours to form; take out the formed product and dry it for 5 hours to obtain the oxygen-increasing synergist.

[0112] Comparative Example 1

[0113] Do not add the long-acting oxygen-increasing granule and the oxygen-increasing synergist, and the others are the same as in Example 1.

[0114] Comparative Example 2

[0115] Do not add the oxygen-increasing synergist, and the others are the same as in Example 1.

[0116] Comparative Example 3

[0117] Do not add the long-acting oxygen-increasing granule, and the others are the same as in Example 1.

[0118] Table 1

[0119]

[0120]

[0121] Through the data analysis of the above examples and comparative examples, the method for high-density live transportation of rotifers of the present invention has a relatively high survival rate.

[0122] The above are only the preferred embodiments of the present invention, and there is no limitation to the present invention in any form. Although the present invention has been disclosed above with the preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can make some changes or modifications to equivalent embodiments with equivalent changes within the scope of the technical solution of the present invention. However, as long as it does not depart from the content of the technical solution of the present invention, any brief modifications, equivalent changes and modifications made to the above embodiments according to the technical essence of the present invention still fall within the scope of the technical solution of the present invention.

Claims

1. A method for high-density live preservation and transportation of rotifers, comprising: Transport material preparation, rotifer capture, quantitative mixing, cooling and oxygenation enhancement, packaging, activation and temporary cultivation; The operation steps are as follows: S1 Transport material preparation: including double-layer polyethylene plastic bags, polyethylene plastic bags, polystyrene foam boxes with lids, cartons, seawater ice, long-acting oxygenation granules, oxygenation enhancer and oxygen filling equipment; S2 Rotifer capture: Select an appropriate time to capture rotifers. Capture rotifers through a suspended water pump and a 200-mesh long nylon mesh bag. After the live rotifers are filtered and dried, they are temporarily stored for later use; S3 Quantitative mixing: After quantifying the rotifers, mix the rotifers with the original pond seawater in a ratio of 1:1 by volume, and then put them into a double-layer polyethylene plastic bag with dimensions of 40 cm × 60 cm and mix well; S4 Cooling and oxygenation enhancement: Put 2000 - 2500 parts of crushed seawater ice into a plastic seal bag and seal it, then put it into a double-layer polyethylene plastic bag containing 5 - 10 L of rotifers for cooling; put 50 - 100 parts of long-acting oxygenation granules and 50 - 100 parts of oxygenation enhancer, and then fill it with oxygen and seal it; S5 Packaging: Pack in the order of double-layer polyethylene plastic bag - polyethylene plastic bag - foam box - carton. Among them, a combination of seawater ice bottles and crushed seawater ice is used to fill the gap between the box body and the sealed plastic bag. Cover the lid and seal it with tape, and then put it into the carton and seal it. The sizes of the plastic box and the carton are 30 L; Activation and temporary rearing of S6: After the rotifers arrive at the destination, remove the packing box, take out the crushed ice in the double-layer polyethylene plastic bag, reseal it, and put it into the temporary rearing pond to slowly warm up. The water temperature of the temporary rearing pond is 20-25°C, and the salinity is 20-30‰; when the temperature difference between the temperature inside the bag and the water temperature of the stocking pond does not exceed 5°C, the rotifers can be put into the pond for activation, with a density of 1-2 kg / m 3 , before the rotifers enter the pond, sprinkle photosynthetic bacteria, EM bacteria and vitamin C to reduce the stress response of the rotifers and improve the survival rate; The oxygenation enhancer is prepared from sodium percarbonate, 6,6'-diamino trehalose, and epoxy soybean oil.

2. The method for high-density live preservation and transportation of rotifers according to claim 1, characterized in that: The rotifer capture time in S2 is preferably during the breeding period of aquatic fry from March to May or from September to November, preferably in the morning or afternoon, avoiding the time with higher temperature at noon.

3. A method for high-density live transportation of rotifers according to claim 1, characterized in that: The raw material seawater salinity of the crushed seawater ice in S4 is 20 - 32%.

4. A method for high-density live preservation and transportation of rotifers according to claim 1, characterized in that: The water temperature of the temporary cultivation pond in S6 is 23 - 25°C, and the salinity is 20 - 30%.

5. A method for high-density live transportation of rotifers according to claim 1, characterized in that: The concentration of photosynthetic bacteria in the temporary cultivation pond in S6 is 50 - 100 μL / L, the concentration of EM bacteria is 15 - 30 μL / L, and the concentration of vitamin C is 5 - 10 mg / L.

6. A method for high-density live preservation and transportation of rotifers according to claim 1, characterized in that: Sprinkle photosynthetic bacteria, EM bacteria, and vitamin C 1 - 12 h before the rotifers enter the pond in S6.

7. A method for high-density live transportation of rotifers according to claim 1, characterized in that: The long-acting oxygenation granules are the granular oxygen of Tianjin Yikang Runze Biotechnology Co., Ltd.

8. A method for high-density live transportation of rotifers according to claim 1, characterized in that: The preparation method of the oxygenation enhancer is as follows: K1: By weight, mix 20 - 30 parts of sodium percarbonate, 4 - 7 parts of 6,6'-diamino trehalose, 0.4 - 0.9 parts of glucose oxidase, 2 - 6 parts of epoxy soybean oil, 2 - 4 parts of sodium bicarbonate, and 200 - 300 parts of deionized water, and stir and mix for 120 - 150 minutes at 50 - 60°C and 250 - 350 revolutions per minute; K2: Then add 0.5 - 2 parts of sodium dodecyl sulfate and 5 - 10 parts of diatomite, and continue to stir at 50 - 60°C for 30 - 40 minutes; stir at 300 - 500 revolutions per minute for 10 - 15 minutes to form a mixed solution; K3: Post-forming treatment step: Transfer the mixed solution to a mold, and let it stand at room temperature for 1 - 2 hours to form; take out the formed product and dry it for 3 - 5 hours to obtain the oxygenation enhancer.

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