Anti-wrinkle firming composition as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of cosmetics, and particularly relates to an anti-wrinkle firming composition as well as a preparation method and application thereof. The composition is prepared from the following raw materials in percentage by mass: 0.1 to 0.2 percent of acetyl hexapeptide-8, 0.3 to 0.5 percent of nostoc sphaeroides polysaccharide extract, 0.051 to 0.069 percent of ethylhexylglycerin, 0.53 to 0.55 percent of phenoxyethanol, 25 to 35 percent of glycerin and the balance of water. The method comprises the following steps: inoculating nostoc sphaeroids kutz into a BG-11 culture medium added with inositol for culturing to obtain nostoc sphaeroids kutz spheroids, inoculating the nostoc sphaeroids kutz spheroids into a BG-11 culture medium added with calcium adenosine diphosphate for culturing to obtain nostoc sphaeroids kutz powder, and finally extracting nostoc sphaeroids kutz polysaccharide by a water extraction and alcohol precipitation method. By improving the cultivation process of the nostoc sphaeroides, the high-quality nostoc sphaeroides powder is obtained, and the yield of the nostoc sphaeroides polysaccharide is further improved. The composition containing the nostoc sphaeroids kutz polysaccharide extract is safe to use and has excellent anti-wrinkle and firming effects.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and particularly relates to an anti-wrinkle and firming composition, a preparation method thereof, and an application thereof. Background Art

[0002] Modern people have a fast-paced life and poor sleep, resulting in lack of skin care. Coupled with ultraviolet rays, electromagnetic radiation, bad living habits (such as smoking and drinking), excessive exercise, mental stress, and excessive intake of highly processed foods and oils, it has jointly caused significant problems such as skin relaxation and aging. Although there are many anti-wrinkle and firming cosmetics on the market, their effects are often unsatisfactory and difficult to meet consumers' urgent needs for skin health and youthfulness.

[0003] Nostoc commune, a precious blue-green alga originating from nature, is famous for its rich active substances such as proteins and polysaccharides, and exhibits excellent physiological activities such as antioxidant, antibacterial, anti-inflammatory, immune-enhancing, and tumor-inhibiting effects. In particular, Nostoc commune is particularly outstanding in antioxidant and moisturizing aspects, providing the possibility for improving skin aging problems. For example, Chinese Patent CN114224819A discloses a Nostoc commune homogenate extract, a preparation method thereof, and an application thereof. Applying this extract to cosmetics can achieve antioxidant, anti-glycation, and moisturizing effects. Chinese Patent CN118490577A discloses a composite algae functional microalgae bead, a preparation method thereof, and an application thereof. The composite algae extract is superimposed and matched with skin care efficacy components with diversified repair and anti-aging effects, such as Nostoc commune extract and Spirulina extract functional microalgae, and has a synergistic effect of soothing repair and anti-aging when applied to skin care products.

[0004] However, the current application of Nostoc commune extract in cosmetics is limited by its extraction process, resulting in uneven quality of the extract, which in turn affects its actual effect in anti-wrinkle and firming. Traditional water extraction and alcohol precipitation methods and other extraction methods can obtain the effective components in Nostoc commune, but have problems such as low extraction rate, many impurities, and poor solubility, and it is difficult to fully exert the full potential of Nostoc commune. Chinese Patent CN110713555A obtained Nostoc commune polysaccharide substances after pre-crushing, enzymatic hydrolysis, acid decolorization, centrifugal filtration, primary alcohol precipitation, redissolution, secondary alcohol precipitation, drying, etc. of Nostoc commune dry products. However, the above extraction methods will cause poor quality of Nostoc commune extract, thus affecting its effect in cosmetics. Therefore, carrying out research and development on Nostoc commune and exploring new methods to improve the yield and quality of Nostoc commune extract can provide an important theoretical basis for the wide application of high-quality Nostoc commune in the cosmetics processing field in China.

[0005] Based on this, the present invention improves the cultivation process of Nostoc commune, provides a Nostoc commune with excellent quality, increases the content of active components such as polysaccharides in Nostoc commune, and further develops an anti-wrinkle and firming composition containing high-quality Nostoc commune extract. Summary of the Invention

[0006] The first object of the present invention is to provide an anti-wrinkle and firming composition, which is safe to use and has excellent anti-wrinkle effects.

[0007] The second object of the present invention is to provide a preparation method of an anti-wrinkle and firming composition.

[0008] The third object of the present invention is to provide an application of an anti-wrinkle and firming composition, which has broad application prospects.

[0009] In order to achieve the above objects, the technical solution adopted by the present invention is as follows:

[0010] An anti-wrinkle and firming composition is composed of the following raw materials in mass percentage: acetyl hexapeptide-8 0.1-0.2%, nostoc commune polysaccharide extract 0.3-0.5%, ethylhexylglycerin 0.051-0.069%, phenoxyethanol 0.53-0.55%, glycerol 25-35%, and the balance is water;

[0011] The preparation process of the nostoc commune polysaccharide extract is as follows:

[0012] (1) Add inositol to the BG-11 medium, and after sterilization treatment, inoculate the nostoc commune algal species into the BG-11 medium for cultivation to obtain nostoc commune spheroids;

[0013] (2) Inoculate the nostoc commune spheroids obtained in step (1) into the BG-11 medium supplemented with calcium adenosine diphosphate, and continue cultivation to obtain nostoc commune. The nostoc commune is dried and pulverized to obtain nostoc commune powder;

[0014] (3) Add the nostoc commune powder obtained in step (2) to water, then add a complex enzyme for enzymatic hydrolysis reaction. After the enzymatic hydrolysis reaction is completed, inactivate the enzyme, filter the enzymatic hydrolysate to obtain a supernatant; add ethanol to the supernatant, and after standing and filtering, obtain a precipitate; dry the precipitate to obtain the nostoc commune polysaccharide extract.

[0015] Preferably, in step (1), the content of inositol in the BG-11 medium is 0.1-0.3 mg / L; the inoculation amount of the nostoc commune algal species is 18-22 g / L; the cultivation conditions are: temperature is 15-20 °C, time is 20-25 d, and light intensity is 100-120 μmol·m -2 ·s -1 .

[0016] Preferably, in step (2), the content of calcium adenosine diphosphate in the BG-11 medium is 1-5 mg / L; the inoculation amount of Nostoc sphaeroides is 18-22 g / L; the culture conditions are as follows: the temperature is 15-20 °C, the time is 5-10 d, and the light intensity is 60-70 μmol·m -2 ·s -1 .

[0017] Preferably, in step (3), the dosage ratio of the Nostoc sphaeroides powder to the complex enzyme is 1 g:(4000-6000) U; the complex enzyme is composed of papain, cellulase, pectinase and neutral protease according to the mass ratio of 1:(1-3):(1-2):(0.5-2.5); the temperature of the enzymatic hydrolysis reaction is 40-45 °C, the time is 7-8 h; the standing time is 4-6 h.

[0018] The preparation method of the above anti-wrinkle and firming composition includes the following steps: mixing the raw materials evenly according to the above mass percentage ratio to obtain the composition.

[0019] The application of the above anti-wrinkle and firming composition is in the preparation of cosmetics.

[0020] Preferably, based on the total mass of the cosmetics, the mass percentage content of the anti-wrinkle and firming composition is 1-5%.

[0021] Preferably, the cosmetics also include the following raw materials with the following mass percentage contents: 4-6% of a moisturizer, 0.01-0.1% of a thickener, and the balance is water.

[0022] Preferably, the moisturizer is selected from one of sodium hyaluronate and 1,3-propanediol; the thickener is selected from one of carbomer, cellulose and xanthan gum.

[0023] Compared with the prior art, the beneficial effects of the present invention mainly lie in:

[0024] The present invention improves the cultivation process of Nostoc sphaeroides. Specifically, in the first cultivation stage of Nostoc sphaeroides, inositol is added to the medium. Inositol can provide nutrients for the growth of Nostoc sphaeroides and promote the stable growth of Nostoc sphaeroides during the cultivation process; in the second cultivation stage, calcium adenosine diphosphate is added to the medium. Calcium adenosine diphosphate is an important bioactive substance, thereby accelerating the growth and development of Nostoc sphaeroides and increasing the polysaccharide content in Nostoc sphaeroides. The properties of Nostoc sphaeroides and the Nostoc sphaeroides polysaccharide extract obtained by the extraction method of the present invention are detected. The results show that the addition of inositol and calcium adenosine diphosphate can significantly improve the hardness and dry-wet weight ratio of Nostoc sphaeroides, making Nostoc sphaeroides have excellent quality, and the purity of the Nostoc sphaeroides polysaccharide extract can reach more than 97.6%, and the yield can reach more than 57.7%.

[0025] The present invention combines the extracted nostoc commune polysaccharide with ingredients such as acetyl hexapeptide-8 to form a composition, which is applied to cosmetics, and the application effect of the cosmetics added with this composition is detected. The results show that after using the cosmetics added with this composition, the wrinkle condition and skin elasticity of the subjects' faces are significantly improved. This result also corroborates that the composition provided by the present invention indeed has good anti-wrinkle and firming effects. Detailed Embodiments

[0026] The following further describes the technical solutions of the present invention in conjunction with specific embodiments. However, those skilled in the art should understand that the following examples are only used to illustrate the present invention and should not be regarded as limiting the present invention. The specific conditions not specified in the examples are carried out according to conventional conditions or the conditions recommended by the manufacturer. The reagents or instruments used, unless otherwise specified, are conventional products obtained through commercial channels.

[0027] 1. Examples

[0028] Example 1

[0029] An anti-wrinkle and firming composition is composed of the following raw materials in mass percentage: 0.15% of acetyl hexapeptide-8, 0.4% of nostoc commune polysaccharide extract, 0.056% of ethylhexylglycerin, 0.54% of phenoxyethanol, 27% of glycerol, and the balance is water.

[0030] Among them, the preparation process of the nostoc commune polysaccharide extract is as follows:

[0031] (1) Add inositol to the BG-11 medium, and the content of inositol in the BG-11 medium is 0.2 mg / L. After sterilization, inoculate the nostoc commune algal species into the above medium at an inoculation amount of 20 g / L, and culture at a temperature of 18°C and a light intensity of 110 μmol·m -2 ·s -1 under an aeration environment for 22 days, and replace the medium every 10 days during the culture process to obtain nostoc commune spheroids.

[0032] (2) Add calcium adenosine diphosphate to the BG-11 medium, and the content of calcium adenosine diphosphate in the BG-11 medium is 3 mg / L. Inoculate the nostoc commune spheroids in step (1) into the BG-11 medium added with calcium adenosine diphosphate at an inoculation amount of 20 g / L, and continue to culture at a temperature of 18°C and a light intensity of 65 μmol·m -2 ·s -1 under an aeration environment for 8 days to obtain nostoc commune. The nostoc commune is dried and pulverized to obtain nostoc commune powder.

[0033] (3) According to the dosage ratio of Nostoc flagelliforme powder to the complex enzyme of 1 g: 5000 U, add the Nostoc flagelliforme powder in step (2) to water with a mass 30 times that of the powder, and then add a complex enzyme composed of papain, cellulase, pectinase and neutral protease in a mass ratio of 1:2:1.5:1. Carry out enzymatic hydrolysis at 42 °C for 7.5 h. After the enzymatic hydrolysis reaction is completed, inactivate the enzyme at 95 °C for 30 min, cool to room temperature, filter the enzymatic hydrolysate to obtain the supernatant. Add ethanol with a volume 3 times that of the supernatant to the supernatant, let it stand for 5 h, filter to obtain the precipitate. Vacuum-dry the above precipitate at 55 °C to prepare the Nostoc flagelliforme polysaccharide extract.

[0034] The preparation method of the above anti-wrinkle and firming composition is as follows:

[0035] Mix the raw materials evenly according to the above mass percentage ratio to obtain the product.

[0036] Example 2

[0037] An anti-wrinkle and firming composition is composed of the following raw materials in mass percentage: acetyl hexapeptide-8 0.1%, Nostoc flagelliforme polysaccharide extract 0.3%, ethylhexylglycerin 0.051%, phenoxyethanol 0.53%, glycerol 25%, and the balance is water.

[0038] Among them, the preparation process of the Nostoc flagelliforme polysaccharide extract is as follows:

[0039] (1) Add inositol to the BG-11 medium, and the content of inositol in the BG-11 medium is 0.3 mg / L. After sterilization, inoculate the Nostoc flagelliforme algal species into the above medium at an inoculation amount of 18 g / L, and carry out culturing at a temperature of 15 °C and a light intensity of 100 μmol·m -2 ·s -1 under an aeration environment for 15 d, and change the medium every 20 d during the culturing process to obtain Nostoc flagelliforme spheroids.

[0040] (2) Add calcium adenosine diphosphate to the BG-11 medium, and the content of calcium adenosine diphosphate in the BG-11 medium is 1 mg / L. Inoculate the Nostoc flagelliforme spheroids in step (1) into the BG-11 medium added with calcium adenosine diphosphate at an inoculation amount of 18 g / L, and continue to culture at a temperature of 15 °C and a light intensity of 60 μmol·m -2 ·s -1 under an aeration environment for 5 d to obtain Nostoc flagelliforme. The Nostoc flagelliforme is dried and pulverized to obtain Nostoc flagelliforme powder.

[0041] (3) According to the dosage ratio of Nostoc commune powder to the composite enzyme of 1 g : 4000 U, add the Nostoc commune powder in step (2) to water with a mass 30 times that of the powder, and then add a composite enzyme composed of papain, cellulase, pectinase, and neutral protease in a mass ratio of 1:1:1:0.5. Carry out enzymatic hydrolysis at 45°C for 8 h. After the enzymatic hydrolysis reaction ends, inactivate the enzyme at 95°C for 30 min, cool to room temperature, filter the enzymatic hydrolysate to obtain the supernatant. Add ethanol with a volume 3 times that of the supernatant to the supernatant, let it stand for 4 h, filter to obtain the precipitate. Dry the precipitate under vacuum at 55°C to prepare the Nostoc commune polysaccharide extract.

[0042] The preparation method of the above anti-wrinkle and firming composition is as follows:

[0043] Mix the raw materials evenly according to the above mass percentage ratio to obtain the composition.

[0044] Example 3

[0045] An anti-wrinkle and firming composition is composed of the following raw materials in mass percentage: acetyl hexapeptide-8 0.2%, Nostoc commune polysaccharide extract 0.5%, ethylhexylglycerin 0.069%, phenoxyethanol 0.55%, glycerol 35%, and the balance is water.

[0046] Among them, the preparation process of the Nostoc commune polysaccharide extract is as follows:

[0047] (1) Add inositol to the BG-11 medium, with the content of inositol in the BG-11 medium being 0.1 mg / L. After sterilization, inoculate the Nostoc commune algal species into the above medium at an inoculation amount of 22 g / L, and culture at a temperature of 20°C and a light intensity of 120 μmol·m -2 ·s -1 under an aeration environment for 25 d, and change the medium every 10 d during the culture process to obtain Nostoc commune spheroids.

[0048] (2) Add calcium adenosine diphosphate to the BG-11 medium, with the content of calcium adenosine diphosphate in the BG-11 medium being 5 mg / L. Inoculate the Nostoc commune spheroids in step (1) into the BG-11 medium added with calcium adenosine diphosphate at an inoculation amount of 22 g / L, and continue to culture at a temperature of 20°C and a light intensity of 70 μmol·m -2 ·s -1 under an aeration environment for 10 d to obtain Nostoc commune. After drying and pulverizing the Nostoc commune, obtain Nostoc commune powder.

[0049] (3) According to the dosage ratio of Nostoc commune powder to the composite enzyme of 1 g: 6000 U, add the Nostoc commune powder in step (2) to water with a mass 30 times that of the powder, and then add a composite enzyme composed of papain, cellulase, pectinase, and neutral protease in a mass ratio of 1:3:2:2.5. Carry out enzymatic hydrolysis at 40 °C for 7 h. After the enzymatic hydrolysis reaction ends, inactivate the enzyme at 95 °C for 30 min, cool to room temperature, filter the enzymatic hydrolysate to obtain the supernatant. Add ethanol with a volume 3 times that of the supernatant to the supernatant, let it stand for 6 h, filter to obtain the precipitate. Dry the precipitate under vacuum at 55 °C to prepare the Nostoc commune polysaccharide extract.

[0050] The preparation method of the above anti-wrinkle and firming composition is as follows:

[0051] Mix the raw materials evenly according to the above mass percentage ratio to obtain the product.

[0052] 2. Comparative examples

[0053] Comparative example 1

[0054] The difference between comparative example 1 and example 1 is that calcium phosphate is used instead of calcium adenosine diphosphate in the preparation process of the Nostoc commune polysaccharide extract, and the rest is the same as example 1.

[0055] Comparative example 2

[0056] The difference between comparative example 2 and example 1 is that adenosine is used instead of calcium adenosine diphosphate in the preparation process of the Nostoc commune polysaccharide extract, and the rest is the same as example 1.

[0057] Comparative example 3

[0058] The difference between comparative example 3 and example 1 is that inositol is not added to the culture medium in preparation step (1) during the preparation process of the Nostoc commune polysaccharide extract, and the rest is the same as example 1.

[0059] Comparative example 4

[0060] The difference between comparative example 4 and example 1 is that calcium adenosine diphosphate is not added to the culture medium in preparation step (2) during the preparation process of the Nostoc commune polysaccharide extract, and the rest is the same as example 1.

[0061] Comparative example 5

[0062] The difference between comparative example 5 and example 1 is that inositol is not added to the culture medium in preparation step (1) and calcium adenosine diphosphate is not added to the culture medium in preparation step (2) during the preparation process of the Nostoc commune polysaccharide extract, and the rest is the same as example 1.

[0063] 3. Experimental examples

[0064] Experimental example 1

[0065] During the preparation process of the Nostoc flagelliforme polysaccharide extracts obtained in Examples 1-3 and Comparative Examples 1-5, the hardness and the dry-wet weight ratio of the Nostoc flagelliforme obtained in step (2) were detected.

[0066] The detection method for hardness is as follows:

[0067] Randomly select 10 Nostoc flagelliforme with the same diameter, dry them with blotting paper, then place the Nostoc flagelliforme bodies on a glass plate, and use a three-scale push-pull force gauge (SF-10N) to measure the pressure required to break the Nostoc flagelliforme. The hardness data is the average value of the pressure required to break 10 Nostoc flagelliforme. The results are shown in Table 1.

[0068] The detection method for the dry-wet weight ratio is as follows:

[0069] Take 10 g of Nostoc flagelliforme, weigh it after drying the surface moisture, which is recorded as the wet weight; after weighing, transfer it to an oven and dry it to a constant weight at 105 °C, record the dry weight values of each group. The calculation formula for the dry-wet weight ratio is: dry-wet weight ratio (%) = dry weight / wet weight × 100%. The results are shown in Table 1.

[0070] Table 1

[0071] Grouping Ratio of dry weight to wet weight (%) Hardness (N) Example 1 1.77±0.02 1.82±0.14 Example 2 1.71±0.03 1.79±0.30 Example 3 1.60±0.03 1.73±0.22 Comparative Example 1 1.39±0.05 1.50±0.51 Comparative Example 2 1.32±0.06 1.41±0.43 Comparative Example 3 0.78±0.07 0.94±0.45 Comparative Example 4 1.04±0.07 1.12±0.62 Comparative Example 5 0.56±0.05 0.75±0.37

[0072] It can be seen from observing Table 1 that the dry-wet weight ratio and hardness of the Nostoc flagelliforme in Examples 1-3 are higher than those in Comparative Examples 1-2. In Comparative Example 1, calcium phosphate was used instead of calcium adenosine diphosphate during the preparation process of the Nostoc flagelliforme extract, and in Comparative Example 2, adenosine was used instead of calcium adenosine diphosphate during the preparation process of the Nostoc flagelliforme extract.

[0073] The dry-wet weight ratio and hardness of the Nostoc flagelliforme in Examples 1-3 are significantly higher than those in Comparative Examples 3-5. In Comparative Example 3, inositol was not added to the culture medium in preparation step (1), in Comparative Example 4, calcium adenosine diphosphate was not added to the culture medium in preparation step (2), and in Comparative Example 5, inositol was not added to the culture medium in preparation step (1) and calcium adenosine diphosphate was not added to the culture medium in preparation step (2). In summary, increasing the dosage of inositol in the first culture stage and increasing the dosage of calcium adenosine diphosphate in the second culture stage can significantly improve the quality of Nostoc flagelliforme.

[0074] Experimental Example 2

[0075] The Nostoc flagelliforme polysaccharide extracts prepared in Examples 1-3 and Comparative Examples 1-5 were detected by the phenol-sulfuric acid method, and the purity of the Nostoc flagelliforme polysaccharide was determined by high performance liquid chromatography. The results are shown in Table 2.

[0076] Table 2

[0077] Grouping Yield of Nostoc commune polysaccharide extract (%) Purity of Nostoc commune polysaccharide extract (%) Example 1 58.6 98.5 Example 2 58.1 97.9 Example 3 57.7 97.6 Comparative Example 1 51.5 97.0 Comparative Example 2 50.8 96.8 Comparative Example 3 47.2 96.1 Comparative Example 4 48.3 96.5 Comparative Example 5 45.1 95.4

[0078] As can be seen from Table 2, the purity of the Nostoc commune polysaccharide extract obtained in the present invention reaches over 97.6%, and the yield reaches over 57.7%.

[0079] Compared with Examples 1-3, the yield of the Nostoc commune polysaccharide extract obtained in Comparative Examples 1-5 is significantly reduced. As can be seen from the above, increasing the inositol dosage in the first cultivation stage and increasing the calcium adenosine diphosphate dosage in the second cultivation stage can significantly improve the quality of Nostoc commune, and thus increase the yield of the Nostoc commune polysaccharide extract.

[0080] 4. Application Examples

[0081] The compositions of Examples 1-3 are applied to the preparation of cosmetics. The following cosmetics are taken as examples in the present invention to illustrate the technical effects that the compositions of the present invention can achieve.

[0082] A serum is composed of the following raw materials in mass percentage: 3% anti-wrinkle and firming composition, 5% 1,3-propanediol, 0.03% carbomer, and the balance is water.

[0083] Application Example 1

[0084] The safety of the serum composed of the compositions of Examples 1-3 is detected by using the human skin patch test. The detection process is as follows:

[0085] A total of 80 subjects with healthy skin, aged 18-30 years old, are randomly selected and randomly divided into 4 groups, with the male-female ratio of 1:1 in each group.

[0086] Patch method: By using the closed patch test method, 0.025 mL of the serum composed of the compositions of Examples 1-3 and water (control group) are placed in the patch tester, and the patch tester is externally applied to the flexor side of the forearm of the subject with a low-sensitization tape. After 24 h, the test substances are removed, and the skin reactions are observed at 0.5 h, 24 h, and 48 h after removal respectively. The results are recorded according to the skin reaction grading standard in the "Technical Specifications for Cosmetics Safety" (2015 Edition), and the results are shown in Table 3.

[0087] Table 3

[0088]

[0089] As can be seen from Table 3, the results of the human skin patch test show that 0 cases of skin adverse reactions occur among 20 people. The above results indicate that the composition of the present invention is safe to use.

[0090] Application Example 2

[0091] Thirty healthy female subjects aged 35-50 years old with similar facial wrinkle degrees are selected and randomly divided into 3 groups.

[0092] Before the experiment, the Antera 3D skin analyzer was used to count the number and area of wrinkles in the wrinkle area of the subjects' cheeks, and the MPA580 skin elasticity tester was used to measure the skin elasticity of the subjects' faces and record it. The essence composed of the compositions of Examples 1-3 was taken and evenly applied to the wrinkle area of the subjects' cheeks (the dosage was 1-2 mL, with a thin layer applied to the cheeks), and it was used once in the morning and once in the evening every day for 4 consecutive weeks. The Antera 3D skin analyzer was used again to count the number and area of wrinkles in the wrinkle area of the subjects' cheeks, and the MPA580 skin elasticity tester was used again to measure the skin elasticity of the subjects' faces and record it. The reduction degree of the number and area of wrinkles in the wrinkle area of each group of subjects and the change in skin elasticity were analyzed. The experimental data were the average values of each group, and the results are shown in Table 4.

[0093] Table 4

[0094]

[0095]

[0096] It can be seen from observing Table 4 that after using the essence added with the compositions of Examples 1-3, the wrinkle condition and skin elasticity of the subjects' faces were significantly improved. That is, the composition can significantly improve the wrinkle condition and skin elasticity of the face.

[0097] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit them. The basic principles and main features of the present invention have been described with specific implementation schemes above. On the basis of the present invention, some modifications or replacements can be made, but these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the present invention claimed.

Claims

1. An anti-wrinkle and firming composition, characterized in that, It is composed of raw materials with the following mass percentages: acetyl hexapeptide-8 0.1-0.2%, nostoc commune polysaccharide extract 0.3-0.5%, ethylhexylglycerin 0.051-0.069%, phenoxyethanol 0.53-0.55%, glycerol 25-35%, and the balance is water; The preparation process of the nostoc commune polysaccharide extract is as follows: (1) Add inositol to the BG-11 medium, inoculate the nostoc commune algal species into the BG-11 medium after sterilization treatment, and culture to obtain nostoc commune spheroids; (2) Inoculate the nostoc commune spheroids in step (1) into the BG-11 medium supplemented with calcium adenosine diphosphate, continue to culture to obtain nostoc commune, dry and crush the nostoc commune to obtain nostoc commune powder; (3) Add the nostoc commune powder in step (2) to water, then add a complex enzyme for enzymatic hydrolysis reaction. After the enzymatic hydrolysis reaction is completed, inactivate the enzyme, filter the enzymatic hydrolysate to obtain a supernatant; add ethanol to the supernatant, stand and filter to obtain a precipitate; dry the precipitate to obtain the nostoc commune polysaccharide extract.

2. The anti-wrinkle and firming composition according to claim 1, wherein In the BG-11 medium described in step (1), the content of inositol is 0.1-0.3 mg / L; the inoculation amount of the Nostoc commune var. flagelliforme algal species is 18-22 g / L; the culture conditions: the temperature is 15-20 °C, the time is 20-25 d, and the light intensity is 100-120 μmol·m -2 ·s -1 .

3. The anti-wrinkle and firming composition according to claim 1, wherein In the BG-11 medium described in step (2), the content of calcium adenosine diphosphate is 1-5 mg / L; the inoculation amount of Nostoc sphaeroides is 18-22 g / L; the culture conditions are as follows: the temperature is 15-20 °C, the time is 5-10 d, and the light intensity is 60-70 μmol·m -2 ·s -1 .

4. The anti-wrinkle and firming composition according to claim 1, wherein In step (3), the dosage ratio of the nostoc commune powder to the complex enzyme is 1 g:(4000-6000) U; the complex enzyme is composed of papain, cellulase, pectinase and neutral protease in a mass ratio of 1:(1-3):(1-2):(0.5-2.5); the temperature of the enzymatic hydrolysis reaction is 40-45 °C, and the time is 7-8 h; the standing time is 4-6 h.

5. The preparation method of the anti-wrinkle and firming composition according to any one of claims 1-4, characterized in that, It includes the following steps: Mix the raw materials evenly according to the above mass percentage ratio to obtain the product.

6. Use of the anti-wrinkle and firming composition according to any one of claims 1-4, characterized in that, Application in the preparation of cosmetics.

7. Use of the anti-wrinkle and firming composition according to claim 6, characterized in that, Based on the total mass of the cosmetics, the mass percentage of the anti-wrinkle and firming composition is 1-5%.

8. Use of the anti-wrinkle and firming composition according to claim 6, characterized in that, The cosmetics also include raw materials with the following mass percentages: humectant 4-6%, thickener 0.01-0.1%, and the balance is water.

9. Use of the anti-wrinkle and firming composition according to claim 6, characterized in that, The humectant is selected from one of sodium hyaluronate and 1,3-propanediol; the thickener is selected from one of carbomer, cellulose and xanthan gum.

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