Livestock and poultry manure fermentation refined organic fertilizer

Abstract

The invention provides a livestock and poultry manure fermentation refined organic fertilizer, and belongs to the technical field of bio-organic fertilizers. A three-stage fermentation process is adopted, firstly, a mixed microbial agent containing microorganisms such as bacillus licheniformis, methanosarcina and bifidobacterium animalis is constructed, high-temperature aerobic fermentation is carried out on the mixed microbial agent and original livestock and poultry manure, and after a primary degradation effect is achieved, the microbial agent is supplemented to carry out normal-temperature aerobic fermentation; on the basis, a fermentation product is diluted, the pH is adjusted, then enzymolysis is conducted, protein is preliminarily hydrolyzed into small molecule peptide, meanwhile, polysaccharide is hydrolyzed into monosaccharide to be utilized by microorganisms, then lactobacillus paracasei, pediococcus pentosaceus, propionibacterium freudenreichii, mucor rouxii and absidia coerulea are inoculated, third-stage mixed fermentation is conducted, and fermentation liquid is obtained; and drying the fermentation product under reduced pressure to obtain a finished product. The fertilizer effectively improves the fertilizer efficiency, can obviously promote the growth of crops, is low in ammonia nitrogen content and sulfide content, and is safe and non-toxic.

Description

Technical Field

[0001] The present invention relates to the technical field of biological organic fertilizers, and specifically to a refined organic fertilizer fermented from livestock and poultry manure sewage. Background Art

[0002] Biological organic fertilizer refers to a type of fertilizer that combines the effects of microbial fertilizers and organic fertilizers, which is composed of specific functional microorganisms and mainly organic materials derived from animal and plant residues (such as livestock and poultry manure, crop straw, etc.), and has undergone harmless treatment and composting.

[0003] The main components of biological organic fertilizers include organic solid waste, such as organic garbage, straw, human, livestock, and poultry manure, cake meal, solid waste generated from agricultural and sideline products and food processing, etc. These components are made through microbial fermentation, deodorization, and complete composting. Its core feature is the addition of specific functional microorganisms (bacterial agents), which can decompose organic matter, release nutrients for plants to absorb and utilize, and at the same time have functions such as disease and pest resistance, and soil improvement.

[0004] The advantages of biological organic fertilizers are that they combine the effects of microbial fertilizers and organic fertilizers. Specifically, the functional classifications of biological organic fertilizers include providing nutrition, disease and pest resistance, and soil improvement. Biological organic fertilizers with the function of providing nutrition mainly include rhizobium fertilizers, nitrogen-fixing bacteria fertilizers, phosphate-solubilizing bacteria fertilizers, etc.; biological organic fertilizers with disease-resistant effects inhibit the reproduction of plant rhizosphere pathogens, improve plant resistance, and reduce the occurrence of diseases through the fermentation of biocontrol bacteria or their combined use; biological organic fertilizers that improve the soil decompose organic matter and release nutrients for plants to absorb and utilize. In the prior art, organic fertilizers prepared from livestock and poultry manure sewage generally have problems such as low fertilizer efficiency, high ammonia nitrogen and sulfide contents. For example, in some commercially available fermented chicken manure fertilizer products, the ammonia nitrogen content is as high as 5.23 mg / g, and the sulfide content is 0.381 µmol / g, which not only has limited fertilizer efficiency but may also have negative impacts on the soil and crops. Summary of the Invention

[0005] The technical problem to be solved by the present invention is that currently, the fertilizer efficiency of organic fertilizers prepared from livestock and poultry manure sewage is generally not high, the promotion effect on crop production is limited, and the ammonia nitrogen content and sulfide content of some products are relatively high, lacking safety guarantee.

[0006] To achieve the above technical objectives, the present invention adopts the following technical solutions: A refined organic fertilizer fermented from livestock and poultry manure sewage, and this organic fertilizer is prepared by the following method: 1) Prepare a mixed microbial agent containing the following weight percentages: Bacillus licheniformis 8% - 10%, Bifidobacterium adolescentis 8% - 10%, Methanosarcina 10% - 12%, Lactobacillus rhamnosus 8% - 10%, Streptococcus thermophilus 4% - 6%, Bifidobacterium animalis 4% - 6%, Leuconostoc pentosum 4% - 6%, Lactobacillus listeri 5% - 7%, Macrococcus caseolyticus 2% - 4%, Alcaligenes viscolactis 5% - 7%, Actinopolyspora halophila 5% - 7%, Hansenula anomala 5% - 7%, Rhizopus nigricans 2% - 4%, with the balance being nutrients; the nutrients include the following components in parts by weight: yeast extract 4 - 6 parts, peptone 1 - 2 parts, soybean cake powder 2 - 3 parts, fish meal 0.5 - 1 part, wood vinegar 0.5 - 1 part, whey protein 0.2 - 0.3 part, potato homogenate 0.2 - 0.3 part; 2) Adjust the water content of livestock and poultry manure to 50% - 60% and the carbon-nitrogen ratio to 20:1 - 25:1. Add the mixed microbial agent thereto and mix evenly. The mass ratio of livestock and poultry manure to the mixed microbial agent is 65:1 - 85:1. Heat up to 50 - 60 °C and perform aerobic fermentation for 8 - 10 d; then turn the pile and cool down to 32 - 35 °C, and supplement the mixed microbial agent at 0.2 - 0.4 times the initial dosage, and continue to maintain aerobic fermentation at 32 - 35 °C for 30 d; 3) Dilute the product obtained in step 2) with water to a viable bacteria count of 10 10 ~10 11 cfu / g. Add bromelain at 250 U / g, papain at 460 U / g, arabinofuranosidase at 310 U / g, pectin lyase at 120 U / g, cyclodextrin glucosyltransferase at 400 U / g, hexose oxidase at 150 U / g, ficin at 90 U / g, and adjust the pH to 7.9 to obtain a fermentation substrate; mix the powder of Lactobacillus paracasei, the powder of Pediococcus pentosaceus, the powder of Propionibacterium freudenreichii, the spore powder of Mucor rouxii, and the spore powder of Absidia coerulea to obtain a mixed spore powder. Mix the mixed spore powder and the fermentation substrate evenly at a ratio of 0.1:20 - 0.1:35, and ferment at a temperature of 28 - 30 °C for 2 - 3 d, and then perform vacuum drying to a water content of 35 - 45% to obtain the organic fertilizer.

[0007] Preferably, in step 1), the weight percentages of Bacillus licheniformis, Bifidobacterium adolescentis, Methanosarcina, Lactobacillus rhamnosus, Streptococcus thermophilus, Bifidobacterium animalis, Leuconostoc pentosum, Lactobacillus listeri, Macrococcus caseolyticus, Alcaligenes viscolactis, Actinopolyspora halophila, Hansenula anomala, and Rhizopus nigricans are 9%, 9%, 11%, 9%, 5%, 5%, 5%, 6%, 3%, 6%, 6%, 6%, and 3% respectively.

[0008] Preferably, the nutrient is composed of the following components in parts by weight: 5 parts of yeast extract, 1.5 parts of peptone, 2.5 parts of soybean cake powder, 0.8 parts of fish meal, 0.7 parts of wood vinegar liquid, 0.22 parts of whey protein, and 0.26 parts of potato homogenate; Preferably, in step 2), the water content of the livestock and poultry manure is adjusted to 55%, and the carbon-nitrogen ratio is 22:1.

[0009] Preferably, in step 2), the mass ratio of the livestock and poultry manure to the mixed microbial inoculum is 70:1.

[0010] Preferably, in step 2), during the 8 - 10 d of aerobic fermentation, the oxygen content in the compost pile is maintained at 9% - 12%; during the 30 d of aerobic fermentation, the oxygen content in the compost pile is maintained at 7% - 9%.

[0011] Preferably, in step 2), it is heated to 55 °C for aerobic fermentation for 9 d; then the pile is turned over to cool down to 33 °C, and a mixed microbial inoculum 0.3 times the initial dosage is added, and aerobic fermentation is continued at 34 °C for 30 d while maintaining the compost.

[0012] Preferably, in step 3), the weight ratio of the Lactobacillus paracasei powder, Pediococcus pentosaceus powder, Propionibacterium freudenreichii powder, Mucor rouxii spore powder, and Absidia coerulea spore powder is 11:3:6:1:2.

[0013] Preferably, in step 3), the mixed microbial powder is uniformly mixed with the fermentation substrate at a ratio of 0.1:25, fermented at a temperature of 29 °C for 3 d, and then dried under reduced pressure to a water content of 40%.

[0014] Preferably, in step 3), during the 2 - 3 d of fermentation, the pH of the fermentation system is controlled at 7.5 - 8.0, and the oxygen content is 12% - 15%.

[0015] The above fermentation process can be carried out using the intelligent aerobic fermentation tank (patent number ZL201721507961.7) produced by the applicant, which can effectively shorten the fermentation time, and the fermentation can be quickly completed in 6 - 15 days. Moreover, it has a small floor area and a short aerobic fermentation cycle, and at the same time overcomes the problem of non - escape of malodorous gases.

[0016] The present invention provides a fermented and refined organic fertilizer from livestock and poultry manure. This technical solution adopts a three-stage fermentation process. First, a mixed bacterial agent containing microorganisms such as Bacillus licheniformis, Methanosarcina, and Bifidobacterium animalis is constructed and subjected to high-temperature aerobic fermentation with the original livestock and poultry manure. After achieving a preliminary degradation effect, the bacterial agent is supplemented and then subjected to room-temperature aerobic fermentation. On this basis, the fermentation product is diluted and the pH is adjusted, and then enzymatic hydrolysis is carried out to preliminarily hydrolyze proteins into small-molecule peptides and polysaccharides into monosaccharides for microbial utilization. Subsequently, Lactobacillus paracasei, Pediococcus pentosaceus, Propionibacterium freudenreichii, Mucor rouxii, and Absidia coerulea are inoculated to carry out the third-stage mixed fermentation. The fermentation product is obtained as a finished product after vacuum drying.

[0017] Among them, the first-stage fermentation: Construct a mixed bacterial agent containing microorganisms such as Bacillus licheniformis, Methanosarcina, and Bifidobacterium animalis, and carry out high-temperature aerobic fermentation with the original livestock and poultry manure. The temperature is controlled at 50 - 60 °C, and the fermentation lasts for 8 - 10 days to achieve a preliminary degradation effect. The second-stage fermentation: Supplement the mixed bacterial agent at 0.2 - 0.4 times the initial dosage and carry out room-temperature aerobic fermentation. The temperature is controlled at 32 - 35 °C, and the fermentation lasts for 30 days to further degrade organic substances. The third-stage fermentation: Dilute the fermentation product and adjust the pH, add various enzymes for enzymatic hydrolysis to hydrolyze proteins into small-molecule peptides and polysaccharides into monosaccharides. Subsequently, microorganisms such as Lactobacillus paracasei and Pediococcus pentosaceus are inoculated and subjected to mixed fermentation for 2 - 3 days, and finally a finished product is obtained after vacuum drying.

[0018] The livestock and poultry manure fermented and refined organic fertilizer prepared by the three-stage fermentation process of the present invention has the characteristics of high-efficiency degradation, low ammonia nitrogen, and low sulfide. The present invention effectively improves the fertilizer efficiency, can significantly promote the growth of crops, and has low ammonia nitrogen content and sulfide content, and is safe and non-toxic. Specific Embodiments

[0019] The specific embodiments of the present invention will be described in detail below. To avoid excessive unnecessary details, the well-known structures or functions will not be described in detail in the following embodiments. The approximate language used in the following embodiments can be used for quantitative expressions, indicating that certain changes in quantity are allowed without changing the basic functions. Unless otherwise defined, the technical and scientific terms used in the following embodiments have the same meanings as commonly understood by those skilled in the art to which the present invention belongs.

[0020] Example 1 A fermented and refined organic fertilizer from livestock and poultry manure, which is prepared by the following method: 1) Prepare a mixed microbial agent containing the following weight percentages: Bacillus licheniformis 8% - 10%, Bifidobacterium adolescentis 8% - 10%, Methanosarcina 10% - 12%, Lactobacillus rhamnosus 8% - 10%, Streptococcus thermophilus 4% - 6%, Bifidobacterium animalis 4% - 6%, Leuconostoc pentosum 4% - 6%, Lactobacillus listeri 5% - 7%, Macrococcus caseolyticus 2% - 4%, Alcaligenes viscolactis 5% - 7%, Actinopolyspora halophila 5% - 7%, Hansenula anomala 5% - 7%, Rhizopus nigricans 2% - 4%, and the balance is nutrients; the nutrients include the following components in parts by weight: yeast extract 4 - 6 parts, peptone 1 - 2 parts, soybean cake powder 2 - 3 parts, fish meal 0.5 - 1 part, wood vinegar 0.5 - 1 part, whey protein 0.2 - 0.3 part, potato homogenate 0.2 - 0.3 part; 2) Adjust the water content of livestock and poultry manure to 50% - 60% and the carbon-nitrogen ratio to 20:1 - 25:1. Add the above-mentioned mixed microbial agent and mix evenly. The mass ratio of livestock and poultry manure to the mixed microbial agent is 65:1 - 85:1. Heat up to 50 - 60 °C for aerobic fermentation for 8 - 10 d; then turn the pile and cool down to 32 - 35 °C, add a mixed microbial agent 0.2 - 0.4 times the initial dosage, and continue to maintain aerobic fermentation at 32 - 35 °C for 30 d; 3) Dilute the product obtained in step 2) with water to a viable bacteria count of 10 10 ~10 11 cfu / g. Add bromelain at 250 U / g, papain at 460 U / g, arabinofuranosidase at 310 U / g, pectin lyase at 120 U / g, cyclodextrin glucosyltransferase at 400 U / g, hexose oxidase at 150 U / g, ficin at 90 U / g, and adjust the pH to 7.9 to obtain a fermentation substrate; mix Lactobacillus paracasei powder, Pediococcus pentosaceus powder, Propionibacterium freudenreichii powder, Mucor rouxii spore powder, and Absidia coerulea spore powder to obtain a mixed spore powder. Mix the mixed spore powder with the fermentation substrate in a ratio of 0.1:20 - 0.1:35, and ferment at a temperature of 28 - 30 °C for 2 - 3 d, and then vacuum dry to a water content of 35% - 45% to obtain the organic fertilizer.

[0021] Example 2 An organic fertilizer refined by fermenting livestock and poultry manure sewage, which is prepared by the following method: 1) Prepare a mixed bacterial agent containing the following weight percentages: Bacillus licheniformis 8% - 10%, Bifidobacterium adolescentis 8% - 10%, Methanosarcina 10% - 12%, Lactobacillus rhamnosus 8% - 10%, Streptococcus thermophilus 4% - 6%, Bifidobacterium animalis 4% - 6%, Leuconostoc pentosum 4% - 6%, Lactobacillus leichmannii 5% - 7%, Megasphaera caseolyticus 2% - 4%, Alcaligenes viscolactis 5% - 7%, Halophilic actinomycetes 5% - 7%, Hansenula 5% - 7%, Rhizopus nigricans 2% - 4%, and the balance is nutrients; the nutrients include the following components in parts by weight: yeast extract 4 - 6 parts, peptone 1 - 2 parts, soybean cake powder 2 - 3 parts, fish meal 0.5 - 1 part, wood vinegar 0.5 - 1 part, whey protein 0.2 - 0.3 part, potato homogenate 0.2 - 0.3 part; 2) Adjust the water content of livestock and poultry manure to 50% - 60% and the carbon-nitrogen ratio to 20:1 - 25:1. Add the mixed bacterial agent thereto and mix evenly. The mass ratio of livestock and poultry manure to the mixed bacterial agent is 65:1 - 85:1. Heat up to 50 - 60 °C for aerobic fermentation for 8 - 10 d; then turn the pile and cool down to 32 - 35 °C, and add a mixed bacterial agent with 0.2 - 0.4 times the initial dosage, and continue to maintain aerobic fermentation at 32 - 35 °C for 30 d; 3) Dilute the product obtained in step 2) with water to a viable bacteria count of 10 10 ~10 11 cfu / g, add bromelain at 250 U / g, papain at 460 U / g, arabinofuranosidase at 310 U / g, pectin lyase at 120 U / g, cyclodextrin glucosyltransferase at 400 U / g, hexose oxidase at 150 U / g, ficin at 90 U / g, and adjust the pH to 7.9 to obtain a fermentation substrate; mix the powder of Lactobacillus paracasei, the powder of Pediococcus pentosaceus, the powder of Propionibacterium freudenreichii, the spore powder of Mucor rouxii, and the spore powder of Absidia coerulea to obtain a mixed spore powder. Mix the mixed spore powder and the fermentation substrate evenly at a ratio of 0.1:20 - 0.1:35, and ferment at a temperature of 28 - 30 °C for 2 - 3 d, and then reduce the pressure and dry to a water content of 35% - 45% to obtain the organic fertilizer.

[0022] In step 1), the weight percentages of Bacillus licheniformis, Bifidobacterium adolescentis, Methanosarcina, Lactobacillus rhamnosus, Streptococcus thermophilus, Bifidobacterium animalis, Leuconostoc pentosum, Lactobacillus listeri, Megasphaera caseolyticus, Alcaligenes viscolactis, Halophilic actinomycetes, Hansenula anomala, and Rhizopus nigricans are 9%, 9%, 11%, 9%, 5%, 5%, 5%, 6%, 3%, 6%, 6%, 6%, and 3% respectively. The nutrient is composed of the following components in parts by weight: 5 parts of yeast extract, 1.5 parts of peptone, 2.5 parts of soybean cake powder, 0.8 part of fish meal, 0.7 part of wood vinegar liquid, 0.22 part of whey protein, and 0.26 part of potato homogenate; in step 2), the moisture content of livestock and poultry manure is adjusted to 55%, and the carbon-nitrogen ratio is 22:1. In step 2), the mass ratio of livestock and poultry manure to the mixed bacterial agent is 70:1. In step 2), during the 8 - 10 d of aerobic fermentation, the oxygen content in the pile body is maintained at 9% - 12%; during the 30 d of aerobic fermentation, the oxygen content in the pile body is maintained at 7% - 9%. In step 2), the temperature is raised to 55 °C for aerobic fermentation for 9 d; then the pile is turned over to cool down to 33 °C, and 0.3 times the initial dosage of the mixed bacterial agent is added, and aerobic fermentation is continued at 34 °C for 30 d. In step 3), the weight ratio of Lactobacillus paracasei powder, Pediococcus pentosaceus powder, Propionibacterium freudenreichii powder, Mucor rouxii spore powder, and Absidia coerulea spore powder is 11:3:6:1:2. In step 3), the mixed bacterial powder is evenly mixed with the fermentation substrate at a ratio of 0.1:25, and fermented at a temperature of 29 °C for 3 d, and then dried under reduced pressure to a water content of 40%. In step 3), during the 2 - 3 d of fermentation, the pH of the fermentation system is controlled at 7.5 - 8.0, and the oxygen content is 12% - 15%.

[0023] Comparative Example 1 Fermented chicken manure fertilizer product produced by Hebei Rundong Fertilizer Co., Ltd.; product model KJ121, the main component is fermented chicken manure.

[0024] Comparative Example 2 Fermented chicken manure fertilizer product produced by Dalian Yonglv Bio-Fertilizer Co., Ltd.; product model AEC - 226, the main component is fermented chicken manure.

[0025] Detection of partial toxic components For the products provided in Example 1, Example 2, Comparative Example 1, and Comparative Example 2, the ammonia nitrogen content and sulfide content in them are respectively detected, and the detection methods are implemented according to the standards of GB / T 7479 - 1987 and GB / T 16489 - 1996. The detection results are shown in Table 1 below.

[0026] Table 1 Detection results of ammonia nitrogen content and sulfide content As shown in Table 1, the organic fertilizers of the present invention (Example 1 and Example 2) are comprehensively lower than the commercially available fermented chicken manure fertilizers (Comparative Example 1 and Comparative Example 2) in terms of both ammonia nitrogen content and sulfide content, proving that the process method of the present invention can significantly reduce the ammonia nitrogen content and sulfide content in livestock and poultry manure, thereby reducing the impact of toxic components on crops to a certain extent and ensuring the safety of cultivated land.

[0027] Fertilizer efficiency detection For the products provided in Example 1, Example 2, Comparative Example 1, and Comparative Example 2, the total nitrogen, total phosphorus, total potassium, and total organic matter contents were respectively detected. The detection method was implemented according to the standard of NYT525-2021, and the detection results are shown in Table 2 below.

[0028] Table 2 Detection results of nutrient content Field planting experiments were carried out on the products provided in Example 1, Example 2, Comparative Example 1, and Comparative Example 2: Tomatoes were cultivated in a vegetable garden. Sowing and seedling raising were carried out on April 2, 2024, and transplanting was carried out on May 14. When transplanting, 500 kg of the corresponding organic fertilizer was applied per mu, and conventional fertilization and routine daily management were carried out; The harvest ended on July 19, and the yield was counted. Among them, the tomato yields of applying the organic fertilizers of Comparative Example 1 and Comparative Example 2 were 2320 jin / mu and 2448 jin / mu respectively, and the tomato yields of applying the organic fertilizers of Example 1 and Example 2 were 5214 jin / mu and 5573 jin / mu respectively, with a yield increase of more than 100%. Thus, it can be seen that the organic fertilizer of the present invention has outstanding technical advantages both in terms of nutrient components and in the actual promotion effect on crop growth.

[0029] Conclusion: Through a three-stage fermentation process, combined with a variety of microorganisms and enzymatic hydrolysis technologies, the present invention successfully prepared a highly efficient and low-toxic fermented refined organic fertilizer from livestock and poultry manure. The experimental results show that the ammonia nitrogen and sulfide contents of this organic fertilizer are significantly lower than those of the received products, and the fertilizer efficiency is significantly improved, which can significantly promote the growth of crops and has broad application prospects.

[0030] The above has described the embodiments of the present invention in detail, but the content described is only the preferred embodiments of the present invention and is not used to limit the present invention. Any modifications, equivalent replacements, and improvements made within the scope of the application of the present invention shall be included within the protection scope of the present invention.

Claims

1. A fermented and refined organic fertilizer for livestock and poultry manure, characterized in that, The organic fertilizer is prepared by the following method: 1) Prepare a mixed bacterial agent containing the following weight percentages: Bacillus licheniformis 8% - 10%, Bifidobacterium adolescentis 8% - 10%, Methanosarcina 10% - 12%, Lactobacillus rhamnosus 8% - 10%, Streptococcus thermophilus 4% - 6%, Bifidobacterium animalis 4% - 6%, Leuconostoc pentosum 4% - 6%, Lactobacillus listeri 5% - 7%, Macrococcus caseolyticus 2% - 4%, Alcaligenes viscolactis 5% - 7%, Actinopolyspora halophila 5% - 7%, Hansenula anomala 5% - 7%, Rhizopus nigricans 2% - 4%, and the balance is nutrients; the nutrients include the following components in parts by weight: yeast extract 4 - 6 parts, peptone 1 - 2 parts, soybean cake powder 2 - 3 parts, fish meal 0.5 - 1 part, wood vinegar 0.5 - 1 part, whey protein 0.2 - 0.3 part, potato homogenate 0.2 - 0.3 part; 2) Adjust the water content of livestock and poultry manure to 50% - 60% and the carbon-nitrogen ratio to 20:1 - 25:1, add the mixed bacterial agent thereto and mix evenly, the mass ratio of livestock and poultry manure to the mixed bacterial agent is 65:1 - 85:1, heat up to 50 - 60 °C and carry out aerobic fermentation for 8 - 10 d; then turn the pile and cool down to 32 - 35 °C, add a mixed bacterial agent 0.2 - 0.4 times the initial dosage, and continue to maintain aerobic fermentation at 32 - 35 °C for 30 d; 3) Dilute the product obtained in step 2) with water to a viable bacteria count of 10 10 ~10 11 cfu / g, add 250 U / g of bromelain, 460 U / g of papain, 310 U / g of arabinofuranosidase, 120 U / g of pectin lyase, 400 U / g of cyclodextrin glucosyltransferase, 150 U / g of hexose oxidase, 90 U / g of ficin thereto, adjust the pH to 7.9 to obtain a fermentation substrate; mix the powder of Lactobacillus paracasei, the powder of Pediococcus pentosaceus, the powder of Propionibacterium freudenreichii, the spore powder of Mucor rouxii, and the spore powder of Absidia coerulea to obtain a mixed spore powder, mix the mixed spore powder and the fermentation substrate evenly at a ratio of 0.1:20 to 0.1:35, ferment at a temperature of 28-30 °C for 2-3 d, and then dry under reduced pressure to a water content of 35-45% to obtain the organic fertilizer.

2. The fermented and refined organic fertilizer for livestock and poultry manure according to claim 1, characterized in that, In step 1), the weight percentages of Bacillus licheniformis, Bifidobacterium adolescentis, Methanosarcina, Lactobacillus rhamnosus, Streptococcus thermophilus, Bifidobacterium animalis, Leuconostoc pentosum, Lactobacillus listeri, Macrococcus caseolyticus, Alcaligenes viscolactis, Actinopolyspora halophila, Hansenula anomala, and Rhizopus nigricans are 9%, 9%, 11%, 9%, 5%, 5%, 5%, 6%, 3%, 6%, 6%, 6%, and 3% respectively.

3. The fermented and refined organic fertilizer for livestock and poultry manure according to claim 1, characterized in that, The nutrients are composed of the following components in parts by weight: yeast extract 5 parts, peptone 1.5 parts, soybean cake powder 2.5 parts, fish meal 0.8 part, wood vinegar 0.7 part, whey protein 0.22 part, potato homogenate 0.26 part.

4. The fermented and refined organic fertilizer for livestock and poultry manure according to claim 1, characterized in that, In step 2), adjust the water content of livestock and poultry manure to 55% and the carbon-nitrogen ratio to 22:

1.

5. A fermented and refined organic fertilizer for livestock and poultry manure according to claim 1, characterized in that, In step 2), the mass ratio of livestock and poultry manure to the mixed bacterial agent is 70:

1.

6. The fermented and refined organic fertilizer for livestock and poultry manure according to claim 1, wherein In step 2), during the 8 - 10 d of aerobic fermentation, maintain the oxygen content in the pile body at 9% - 12%; during the 30 d of aerobic fermentation, maintain the oxygen content in the pile body at 7% - 9%.

7. The fermented and refined organic fertilizer for livestock and poultry manure according to claim 1, wherein In step 2), heat up to 55 °C and carry out aerobic fermentation for 9 d; then turn the pile and cool down to 33 °C, add a mixed bacterial agent 0.3 times the initial dosage, and continue to maintain aerobic fermentation at 34 °C for 30 d.

8. A fermented and refined organic fertilizer for livestock and poultry manure according to claim 1, characterized in that, In step 3), the weight ratio of Lactobacillus paracasei powder, Pediococcus pentosaceus powder, Propionibacterium freudenreichii powder, Mucor rouxii spore powder, and Absidia coerulea spore powder is 11:3:6:1:

2.

9. The fermented and refined organic fertilizer for livestock and poultry manure according to claim 1, characterized in that, In step 3), mix the mixed bacterial powder and the fermentation substrate evenly at a ratio of 0.1:25, ferment at a temperature of 29 °C for 3 d, and then carry out vacuum drying until the water content is 40%.

10. The fermented and refined organic fertilizer for livestock and poultry manure according to claim 1, characterized in that In step 3), during the 2 - 3 d of fermentation, control the pH of the fermentation system at 7.5 - 8.0 and the oxygen content at 12% - 15%.

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