Chitosan-carrageenan blended composite membrane containing sand fruit peel extract and Vc and preparation method of chitosan-carrageenan blended composite membrane

Abstract

The invention discloses a chitosan-carrageenan blended composite membrane containing a sand fruit peel extract and Vc and a preparation method thereof. The preparation method comprises the following steps: preparing the sand fruit peel extract, preparing a chitosan and carrageenan composite membrane forming solution, mixing the sand fruit peel extract and Vc, and finally selecting a proper membrane forming method to prepare the chitosan-carrageenan blended composite membrane. The natural and degradable biological composite membrane with good antioxidant and antibacterial capabilities is prepared. The composite membrane is prepared from the following raw materials in parts by mass: 1 to 3 parts of chitosan, 0.5 to 1.5 parts of carrageenan, 0.1 to 0.5 part of a sand fruit peel extract, 0.1 to 0.3 part of Vc, 0.4 to 1.2 parts of glycerol and 0.01 to 0.5 part of tween-80. The composite film has the characteristics of good mechanical property, strong water retention capacity, obvious antioxidant and antibacterial effects, natural degradability and the like.

Description

Technical Field

[0001] The present invention belongs to the field of food, and specifically relates to a chitosan-carrageenan blend composite film containing crabapple peel extract and Vc and a preparation method thereof. Background Art

[0002] Chitosan is a biopolymer material obtained by deacetylation of chitin, also known as chitamine, deacetylated chitin, etc. It is a high-molecular compound composed of 2-amino-2-deoxy-β-D-glucose units connected by β-1,4 glycosidic bonds. Since there are many hydroxyl groups, N-acetylamino groups, etc. distributed on the molecular chain of chitin, hydrogen bonds are formed within and between molecules, increasing the spatial structure of the molecular chain. Therefore, it is insoluble in water and general solvents, making chitin inactive and severely limiting its application scope. The deacetylation treatment changes the spatial molecular structure of chitin and affects its chemical properties. However, through the deacetylation method, chitosan not only increases its solubility but also exhibits some special properties, such as film-forming property, biocompatibility, and antibacterial property, etc.

[0003] Carrageenan is a highly sulfated polysaccharide extracted from red seaweeds and mainly composed of macromolecular dietary fiber. Due to its properties of stability and gelation, it can be used to increase the viscosity of food and can also be used to make edible films to increase the plasticity and ductility of the films.

[0004] As waste in agricultural production, crabapple peel is rich in active substances such as polyphenols, and its components are mainly flavonoids, flavonols, anthocyanins, etc. Research shows that the content of flavonoids in the outer skin of crabapple is much higher than that in the inner pulp, and the ethanol extract of crabapple peel can reach 10%, which has strong antioxidant and antibacterial effects. As waste in the processing and production of crabapple juice, if crabapple peel can be developed and utilized, it can not only protect the environment and save resources, but also increase the added value of crabapple peel and create good economic and social benefits.

[0005] Therefore, using crabapple peel extract and Vc mixed into chitosan-carrageenan solution to make a natural degradable biofilm with antioxidant and antibacterial effects can not only achieve the purpose of environmental protection but also realize the reuse of waste and create good economic benefits. Summary of the Invention

[0006] The purpose of the present invention is to provide a chitosan-carrageenan bio-composite film with natural antioxidants or antibacterial agents, and this method has significant significance for solving the problems of short storage period, color browning of chilled meat, and low utilization rate of industrial production waste.

[0007] The specific technical solution of the present invention is described as follows:

[0008] A chitosan-carrageenan blend composite film containing sand fruit peel extract and Vc. The composite film is composed of the following raw materials in mass percentages: 1-3 parts of chitosan, 0.5-1.5 parts of carrageenan, 0.1-0.5 parts of sand fruit peel extract, 0.1-0.3 parts of Vc, 0.4-1.2 parts of glycerol, and 0.01-0.5 parts of Tween-80.

[0009] The viscosity of the chitosan is >400 mPa.s, and the degree of deacetylation is 85%-95%

[0010] The carrageenan is food grade.

[0011] The preparation method of the sand fruit peel extract is to use purple sand fruit peel as the raw material, crush it, extract it with 70% ethanol, evaporate the filtrate by vacuum rotation at 50°C, and then obtain the sand fruit peel extract powder by freeze-drying, and store it at -20°C for later use. Before use, grind it and pass through a 200-mesh sieve.

[0012] The antioxidant is Vc.

[0013] The plasticizer is glycerol.

[0014] The surfactant is Tween-80.

[0015] The chitosan-carrageenan blend composite film containing sand fruit peel extract and Vc and its preparation method are composed of the following raw materials in mass percentages: 1-3 parts of chitosan, 0.5-1.5 parts of carrageenan, 0.1-0.5 parts of sand fruit peel extract, 0.1-0.3 parts of Vc, 0.4-1.2 parts of glycerol, and 0.01-0.5 parts of Tween-80.

[0016] The preparation method of the chitosan-carrageenan blend composite film containing sand fruit peel extract and Vc is realized by the following steps:

[0017] (1) Dissolve chitosan in 100 mL of 1% acetic acid solution, and stir in a 60°C water bath until the chitosan is completely dissolved;

[0018] (2) Add carrageenan, glycerol, and Tween-80, and stir and mix evenly to obtain a mixed solution;

[0019] (3) Then add an aqueous solution of sand fruit peel extract and Vc, stir evenly, and then perform vacuum degassing to obtain a composite film-forming solution;

[0020] (4) Take 20 mL of the composite film-forming solution and spread it evenly in a 15×15 cm plexiglass box, put it into a dryer, and dry it at 60°C for more than 4 hours to form a film;

[0021] (5) Immerse the composite film in a 1% sodium hydroxide solution. After the film detaches from the plexiglass box, wash it with distilled water and finally air-dry it to obtain the chitosan-carrageenan blend composite film with antioxidant and antibacterial effects.

[0022] The chitosan-carrageenan composite film based on apple peel extract and Vc prepared by the said method is applied in the field of food packaging. Specific embodiments

[0022] The following further elaborates on the present invention in conjunction with embodiments, but the scope of protection required by the present invention is not limited to the scope shown in the embodiments.

[0023] Embodiment 1

[0024] A chitosan-carrageenan blend composite film containing apple peel extract and Vc has the following formulation: 1 part of chitosan, 1.5 parts of carrageenan, 0.1 part of apple peel extract, 0.1 part of Vc, 0.4 part of glycerol, 0.01 part of Tween-80;

[0025] A chitosan-carrageenan blend composite film containing apple peel extract and Vc and its preparation method:

[0026] The preparation method of the apple peel extract uses purple apple peel as the raw material, which is crushed and then extracted with 70% ethanol. The filtrate is rotary-evaporated under vacuum at 50 °C, and then freeze-dried to obtain the apple peel extract powder, which is stored at -20 °C for later use. Before use, it is ground and passed through a 200-mesh sieve.

[0027] The preparation method of a chitosan-carrageenan blend composite film containing apple peel extract and Vc is achieved by the following steps:

[0028] (1) Dissolve chitosan in 100 mL of 1% acetic acid solution and stir in a 60 °C water bath until the chitosan is completely dissolved;

[0029] (2) Add carrageenan, glycerol and Tween-80, and stir and mix evenly to obtain a mixed solution;

[0030] (3) Then add the aqueous solutions of apple peel extract and Vc, stir evenly, and then degas under vacuum to obtain a composite film-forming solution;

[0031] (4) Take 20 mL of the said composite film-forming solution and spread it evenly in a 15×15 cm plexiglass box, put it in a dryer, and dry it at 60 °C for more than 4 hours to form a film;

[0032] (5) Immerse the composite film in a 1% sodium hydroxide solution. After the film detaches from the plexiglass box, wash it with distilled water and finally air-dry it to obtain the chitosan-carrageenan blend composite film with antioxidant and antibacterial effects.

[0033] Example 2

[0034] A chitosan-carrageenan blend composite film containing sand peel extract and Vc is composed of the following raw materials in mass percentages: 2 parts of chitosan, 0.5 part of carrageenan, 0.3 part of sand peel extract, 0.2 part of Vc, 1 part of glycerol, and 0.5 part of Tween-80.

[0035] A chitosan-carrageenan blend composite film containing sand peel extract and Vc and its preparation method are realized by the following steps:

[0036] The preparation method of the sand peel extract uses purple sand peel as the raw material. After being crushed, it is extracted with 70% ethanol. The filtrate is rotary evaporated under vacuum at 50°C, and then freeze-dried to obtain the sand peel extract powder, which is stored at -20°C for later use. Before use, it is ground and passed through a 200-mesh sieve.

[0037] Preparation method of a chitosan-carrageenan blend composite film containing sand peel extract and Vc:

[0038] (1) Dissolve chitosan in 100 mL of 1% acetic acid solution and stir in a 60°C water bath until the chitosan is completely dissolved;

[0039] (2) Add carrageenan, glycerol, and Tween-80, stir and mix evenly to obtain a mixed solution;

[0040] (3) Then add an aqueous solution of sand peel extract and Vc, stir and mix evenly, and then degas under vacuum to obtain a composite film-forming solution;

[0041] (4) Take 20 mL of the composite film-forming solution and spread it evenly in a 15×15 cm plexiglass box, put it in a dryer, and dry at 60°C for more than 4 hours to form a film;

[0042] (5) Immerse the film in a 1% sodium hydroxide solution. After the film falls off from the plexiglass box, wash it with distilled water, and finally air-dry it to obtain the chitosan-carrageenan blend composite film with antioxidant and antibacterial effects.

Claims

1. A chitosan-carrageenan blend composite film containing sand peel extract and Vc, characterized in that, Using chitosan as the film-forming material, carrageenan as the auxiliary film-forming material, the extract of sand apple peel as the bacteriostatic agent or antioxidant, Vc as the antioxidant, adding a certain amount of plasticizer and surfactant, and finally selecting a suitable film-forming method to prepare a natural degradable bio-composite film with good antioxidant and bacteriostatic abilities.

2. The chitosan-carrageenan blend composite film containing sand peel extract and Vc as described in claim 1, characterized in that: The viscosity of the chitosan is >400 mPa·s, and the degree of deacetylation is 85%-95%.

3. The chitosan-carrageenan blend composite film containing sand peel extract and Vc as described in claim 1 is characterized in that: The carrageenan is food grade.

4. The chitosan-carrageenan blend composite film containing sand pericarp extract and Vc as described in claim 1 is characterized in that: The preparation method of the extract of sand apple peel is using purple sand apple peel as the raw material, crushing it, extracting it with 70% ethanol, rotary evaporating the filtrate under vacuum at 50 °C, then freeze-drying to obtain the extract powder of sand apple peel, and storing it at -20 °C for later use. Grind it and pass through a 200-mesh sieve before use.

5. The chitosan-carrageenan blend composite film containing sandy pericarp extract and Vc as described in claim 1, characterized in that: The antioxidant is Vc.

6. The chitosan-carrageenan blend composite film containing sand peel extract and Vc as described in claim 1, characterized in that: The plasticizer is glycerol.

7. The chitosan-carrageenan blend composite film containing sand peel extract and Vc as claimed in claim 1, characterized in that: The surfactant is Tween-80.

8. The chitosan-carrageenan blend composite film containing sand peel extract and Vc as described in claim 1 is characterized in that: It is composed of the following raw materials in mass percentages: 1-3 parts of chitosan, 0.5-1.5 parts of carrageenan, 0.1-0.5 parts of the extract of sand apple peel, 0.1-0.3 parts of Vc, 0.4-1.2 parts of glycerol, 0.01-0.5 parts of Tween-80.

9. The preparation method of the chitosan-carrageenan blend composite film containing sand peel extract and Vc according to any one of claims 1 to 8, characterized in that: It includes the following steps: (1) Dissolve chitosan in 100 mL of 1% acetic acid solution, and stir it in a water bath at 60 °C until the chitosan is completely dissolved; (2) Add carrageenan, glycerol and Tween-80, and stir and mix evenly to obtain a mixed solution; (3) Then add the aqueous solution of the extract of sand apple peel and Vc, stir and mix evenly, and then degas under vacuum to obtain a composite film-forming solution; (4) Take 20 mL of the composite film-forming solution and spread it evenly in a 15×15 cm plexiglass box, put it into a dryer, and dry it at 60 °C for more than 4 hours to form a film; (5) Immerse the composite film in 1% sodium hydroxide solution, wait until the film falls off from the plexiglass box, then wash it with distilled water, and finally air-dry it to obtain the chitosan-carrageenan blend composite film containing the extract of sand apple peel and Vc.

10. The application of the chitosan-carrageenan blend composite film containing the extract of sand apple peel and Vc described in any one of claims 1 to 8 or the chitosan-carrageenan composite film based on the extract of sand apple peel and Vc prepared by the preparation method described in claim 9 in the field of food packaging.