Anti-allergy soothing composition as well as preparation method and application thereof

Abstract

The invention discloses an anti-allergy soothing composition and a preparation method and application thereof.The anti-allergy soothing composition is prepared from, by mass, 15%-30% of basic carriers and 70%-85% of plant active ingredients, and the plant active ingredients are prepared from, by mass, 15-30 parts of radix stephaniae tetrandrae, 5-15 parts of radix gentianae, 15-20 parts of Chinese herbaceous peony and 10-15 parts of fructus kochiae; 5 to 10 parts of perilla frutescens; according to the preparation method of the anti-allergy soothing composition, plant active ingredients are extracted in a pressurizing, heating and pressure maintaining extraction mode. According to the composition, the stephania tetrandra, the radix gentianae, the Chinese herbaceous peony, the fructus kochiae and the purple perilla serve as plant active ingredients of the anti-allergy soothing composition, the synergistic effect is achieved in the anti-allergy soothing aspect, the rapid anti-allergy soothing effect is achieved, and meanwhile the basic carrier is matched so that moisturizing and barrier repairing can be enhanced.

Description

Technical Field

[0001] The present invention relates to the field of biomedicine technology, and in particular to an anti-allergic and soothing composition and a preparation method and application thereof. Background Art

[0002] Skin sensitivity is a common skin condition, characterized by symptoms such as redness, stinging, burning, and itching, which is mainly caused by factors such as impaired barrier function, neurovascular hyperreactivity, and immune system abnormalities. Common causes include environmental factors (ultraviolet rays, pollution, temperature and humidity changes), chemical irritation (cosmetic ingredients, preservatives, surfactants), and physiological factors (hormone levels, genetic susceptibility).

[0003] At present, the anti-allergic soothing products on the market mainly include antihistamine / anti-inflammatory ingredients (such as dipotassium glycyrrhizinate, bisabolol), neuromodulatory ingredients (such as 4-tert-butyl cyclohexanol, acetyl dipeptide-1) and physical barrier repair ingredients (ceramide, cholesterol), but these ingredients all have certain limitations. Among them, antihistamine / anti-inflammatory ingredients are effective in the short term and cannot repair the barrier; neuromodulatory ingredients have limited effects on immune sensitivity; physical barrier repair ingredients take a long time to take effect and need to be used for a long time. Summary of the invention

[0004] The present invention provides an anti-allergic soothing composition and a preparation method and application thereof, so as to solve the limitation problems of slow effect and limited effect of current anti-allergic soothing products.

[0005] In order to solve the above technical problems, an embodiment of the present invention provides an anti-allergic soothing composition, which is composed of 15% to 30% of a basic carrier and 70% to 85% of a plant active ingredient by mass percentage, and the plant active ingredient is composed of the following raw materials by mass percentage:

[0006] 15-30 parts of Stephania tetrandra, 5-15 parts of Gentiana scabra, 15-20 parts of Paeonia lactiflora, 10-15 parts of Kochia scoparia, 5-10 parts of Perilla frutescens;

[0007] The preparation method of the anti-allergic soothing composition comprises the following steps:

[0008] Step S1, crushing and sieving Stephania tetrandra, Gentiana scabra, Paeonia lactiflora, Kochia scoparia and Perilla frutescens into a mixed powder, filling the mixed powder into an extraction chamber with a microporous filter outlet, and lightly pressing it into a loose cake shape;

[0009] Step S2, pressurizing and injecting a phosphate buffer solution preheated at 70°C to 80°C as a first extract into the extraction chamber at a pressure of 10MPa to 20MPa, heating the extraction chamber to 85°C to 92°C, and maintaining the pressure for 25min to 40min before releasing the first extract under pressure to obtain a first extract;

[0010] Step S3, inject ethanol aqueous solution as the second extraction liquid into the extraction chamber under pressure, with the pressure ranging from 15 MPa to 30 MPa, heat the extraction chamber to 78°C to 85°C, and keep the pressure for 25 min to 40 min, and then release the second extraction liquid under pressure to obtain the second extract.

[0011] Step S4, mix the first extract and the second extract with the basic carrier, homogenize and adjust the pH to 5.5 to 6.0 to obtain the anti-allergy and soothing composition.

[0012] Stephania tetrandra S. Moore involved in the present invention is a herbaceous vine of the genus Stephania in the Menispermaceae family. According to "Flora of China", its "fleshy main root is used as medicine, called Stephania tetrandra S. Moore, bitter and pungent in taste, cold in nature, dispelling wind and dampness, promoting diuresis and relieving stranguria. It contains various alkaloids". Modern medical research has found that the alkaloids tetrandrine, fangchinoline and cepharanthine extracted from the root have antipyretic, analgesic, anti-inflammatory and blood pressure-lowering effects.

[0013] Gentiana scabra Bunge involved in the present invention is a perennial herb of the genus Gentiana in the Gentianaceae family. Gentiana scabra Bunge is bitter in taste and cold in nature; it belongs to the liver and gallbladder meridians; it has the effects of clearing heat and drying dampness, purging liver and gallbladder fire. Gentiana scabra Bunge also has effects such as antifungal, antibacterial, antimalarial, anti-inflammatory, liver protection and sedation.

[0014] Paeonia lactiflora Pall. involved in the present invention is a perennial herb of the genus Paeonia in the Paeoniaceae family. The root of Paeonia lactiflora Pall. can be used as medicine, called "white peony root", which has the effects of analgesia, spasmolysis, removing stasis and dredging menstruation.

[0015] Fructus Kochiae involved in the present invention is the dried ripe fruit of Kochia scoparia (L.) Schrad. of the Chenopodiaceae family. Fructus Kochiae is pungent and bitter in taste and cold in nature; it belongs to the kidney and bladder meridians; it has the effects of clearing heat and promoting diuresis, dispelling wind and relieving itching; it can be used for dysuria, pudendal itching with leukorrhea, rubella, eczema and skin pruritus.

[0016] Perilla frutescens (L.) Britt. involved in the present invention is an annual erect herb of the genus Perilla in the Lamiaceae family. It is recorded in "Huahui Yanyu" that Perilla frutescens (L.) Britt. has the effects of relieving exterior syndrome and dispelling cold, regulating qi and widening the middle, preventing miscarriage, etc., and is mainly used for treating symptoms such as wind-cold cold, chest distress and vomiting, fetal movement restlessness, etc.

[0017] In some of the embodiments, the plant active ingredients are composed of the following raw materials by mass: 18 parts of Stephania tetrandra S. Moore, 12 parts of Gentiana scabra Bunge, 20 parts of Paeonia lactiflora Pall., 13 parts of Fructus Kochiae and 8 parts of Perilla frutescens (L.) Britt.

[0018] In some of the embodiments, the basic carrier includes one or more of ceramide, squalane, hyaluronic acid and panthenol.

[0019] In some of the embodiments, the basic carrier is composed of the following raw materials in a mass ratio: panthenol: hyaluronic acid: squalane = 5:2:8.

[0020] In some of these embodiments, the particle size of the mixed powder is 300 mesh to 450 mesh.

[0021] In some of these embodiments, the pore size of the microporous filter screen is 15 μm to 20 μm.

[0022] In some of these embodiments, in step S2, the pH of the phosphate buffer solution is 6.0. To pressurize and release the first extract, the liquid outlet of the microporous filter screen is opened, and air is pressurized and injected into the extraction chamber for 30 s to 120 s, and then the phosphate buffer solution is pressurized and injected into the extraction chamber for 30 s to 120 s.

[0023] In some of these embodiments, in step S3, the concentration of the aqueous ethanol solution is 30%. To pressurize and release the second extract, the liquid outlet of the microporous filter screen is opened, and air is pressurized and injected into the extraction chamber for 30 s to 120 s, and then the aqueous ethanol solution is pressurized and injected into the extraction chamber for 30 s to 120 s.

[0024] An embodiment of the present invention also provides an application of an anti-allergy and soothing composition in the preparation of an anti-allergy and soothing product, and the anti-allergy and soothing product includes a facial mask, essence, lotion, gel, aqueous solution or cream.

[0025] Compared with the prior art, the present invention has the following beneficial effects:

[0026] The composition of the present invention uses Stephania tetrandra, Gentiana scabra, Paeonia lactiflora, Kochia scoparia and Perilla frutescens as the plant active ingredients of the anti-allergy and soothing composition, which can reduce allergic reactions, reduce skin erythema and stinging sensations, soothe neurogenic inflammation, and repair the skin barrier, achieving a rapid anti-allergy and soothing effect; combined with a basic carrier, it can further enhance moisturization and barrier repair.

[0027] The preparation method of the present invention adopts a pressurized heating and pressure-holding extraction method, uses a phosphate buffer solution instead of pure water to effectively extract water-soluble components, uses an aqueous ethanol solution to effectively extract lipophilic components, high pressure promotes the rupture of cell walls to improve extraction efficiency, temperature control ensures that volatile components such as perillaldehyde can be effectively retained, and pressure-holding extraction can enrich small molecule active substances, realizing the effective extraction of plant active ingredients. Detailed Embodiments

[0028] In order to make the above objects, features and advantages of the present invention more obvious and understandable, the following detailed description of the specific embodiments of the present invention is given. Many specific details are set forth in the following description in order to fully understand the present invention. However, the present invention can be implemented in many other ways different from those described herein, and those skilled in the art can make similar improvements without departing from the connotation of the present invention. Therefore, the present invention is not limited by the specific embodiments disclosed below.

[0029] As used herein, the term "prepared from" is synonymous with "comprising". The terms "comprising", "including", "having", "containing" or any other variation thereof as used herein are intended to cover non-exclusive inclusion. For example, a composition, step, method, article or apparatus comprising the listed elements need not be limited to those elements, but may include other elements not expressly listed or elements inherent to such composition, step, method, article or apparatus.

[0030] When an equivalent, concentration, or other value or parameter is expressed as a range, a preferred range, or a range defined by a series of upper preferred values and lower preferred values, this should be understood to specifically disclose all ranges formed by any pairing of any upper range limit or preferred value with any lower range limit or preferred value, whether or not the ranges are separately disclosed. For example, when the range "1 to 5" is disclosed, the described range should be interpreted to include the ranges "1 to 4", "1 to 3", "1 to 2", "1 to 2 and 4 to 5", "1 to 3 and 5", etc. When a numerical range is described herein, unless otherwise stated, the range is intended to include its end values and all integers and fractions within the range.

[0031] Furthermore, the indefinite articles "a" and "an" before an element or component of the present invention do not limit the quantity requirement (i.e., the number of occurrences) of the element or component. Therefore, "a" or "an" should be interpreted to include one or at least one, and the singular form of an element or component also includes the plural form, unless the quantity clearly refers to the singular form.

[0032] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the technical field to which this invention belongs. The terms used in the description of the present invention herein are for the purpose of describing specific embodiments only and are not intended to limit the present invention. The term "and / or" used herein includes any and all combinations of one or more of the related listed items.

[0033] Example 1

[0034] An anti-allergy and soothing composition, the anti-allergy and soothing composition is composed of 15% of a base carrier and 85% of a plant active ingredient by mass percentage, and the base carrier is composed of raw materials in the following mass ratio: panthenol: hyaluronic acid: squalane = 5:2:8; the plant active ingredient is composed of the following raw materials by mass parts:

[0035] Stephania tetrandra 15 parts, Gentiana scabra 5 parts, Paeonia lactiflora 15 parts, Kochia scoparia 10 parts, Perilla frutescens 5 parts;

[0036] The preparation method of the anti-allergy and soothing composition comprises the following steps:

[0037] Step S1: After pulverizing Stephania tetrandra, Gentiana scabra, Paeonia lactiflora, Kochia scoparia, and Perilla frutescens and passing them through a sieve to mix them into a 300-mesh mixed powder, fill the extraction chamber with a microporous filter screen outlet with the mixed powder and gently press it into a loose cake shape. The aperture of the microporous filter screen is 20 μm.

[0038] Step S2: Pressurize and inject a pH phosphate buffer solution with a preheating temperature of 70°C into the extraction chamber as the first extraction liquid, with a pressurization pressure of 10 MPa. Then raise the temperature of the extraction chamber to 85°C, keep the pressure for 40 min, then open the outlet of the microporous filter screen, inject air into the extraction chamber under pressure for 30 s, and then inject the phosphate buffer solution into the extraction chamber under pressure for 120 s to obtain the first extraction liquid.

[0039] Step S3: Pressurize and inject a 30% ethanol aqueous solution into the extraction chamber as the second extraction liquid, with a pressurization pressure of 15 MPa. Then raise the temperature of the extraction chamber to 78°C, keep the pressure for 40 min, then open the outlet of the microporous filter screen, inject air into the extraction chamber under pressure for 30 s, and then inject the ethanol aqueous solution into the extraction chamber under pressure for 120 s to obtain the second extraction liquid.

[0040] Step S4: Mix the first extraction liquid, the second extraction liquid with the basic carrier, homogenize, and adjust the pH to 5.5 - 6.0 to obtain the anti-allergy and soothing composition.

[0041] Example 2

[0042] An anti-allergy and soothing composition, the anti-allergy and soothing composition consists of 30% of a basic carrier and 70% of a plant active ingredient by mass percentage. The basic carrier consists of raw materials in the following mass ratio: panthenol: hyaluronic acid: squalane = 5:2:8; the plant active ingredient consists of the following raw materials by mass parts:

[0043] 30 parts of Stephania tetrandra, 15 parts of Gentiana scabra, 20 parts of Paeonia lactiflora, 15 parts of Kochia scoparia, 10 parts of Perilla frutescens;

[0044] The preparation method of the anti-allergy and soothing composition includes the following steps:

[0045] Step S1: After pulverizing Stephania tetrandra, Gentiana scabra, Paeonia lactiflora, Kochia scoparia, and Perilla frutescens and passing them through a sieve to mix them into a 450-mesh mixed powder, fill the extraction chamber with a microporous filter screen outlet with the mixed powder and gently press it into a loose cake shape. The aperture of the microporous filter screen is 15 μm.

[0046] Step S2: Pressurize and inject a pH phosphate buffer solution with a preheating temperature of 80°C into the extraction chamber as the first extraction liquid, with a pressurization pressure of 20 MPa. Then raise the temperature of the extraction chamber to 92°C, keep the pressure for 25 min, then open the outlet of the microporous filter screen, inject air into the extraction chamber under pressure for 120 s, and then inject the phosphate buffer solution into the extraction chamber under pressure for 30 s to obtain the first extraction liquid.

[0047] Step S3: Inject 30% ethanol aqueous solution as the second extraction liquid into the extraction chamber under pressure. The pressure is 30 MPa. Heat the extraction chamber to 85 °C, keep the pressure for 25 min, then open the liquid outlet of the microporous filter screen, inject air into the extraction chamber under pressure for 120 s, and then inject ethanol aqueous solution into the extraction chamber under pressure for 30 s to obtain the second extraction liquid.

[0048] Step S4: Mix the first extraction liquid, the second extraction liquid and the basic carrier, homogenize and adjust the pH to 5.5 - 6.0 to obtain the anti-allergy and soothing composition.

[0049] Example 3

[0050] An anti-allergy and soothing composition, which is composed of 18% basic carrier and 82% plant active ingredients by mass percentage. The basic carrier is composed of raw materials with the following mass ratio: panthenol: hyaluronic acid: squalane = 5:2:8; the plant active ingredients are composed of the following raw materials by mass parts:

[0051] 18 parts of Stephania tetrandra, 12 parts of Gentiana scabra, 20 parts of Paeonia lactiflora, 13 parts of Kochia scoparia, 8 parts of Perilla frutescens;

[0052] The preparation method of the anti-allergy and soothing composition includes the following steps:

[0053] Step S1: After pulverizing, sieving and mixing Stephania tetrandra, Gentiana scabra, Paeonia lactiflora, Kochia scoparia and Perilla frutescens into a 400-mesh mixed powder, fill the extraction chamber with a microporous filter screen liquid outlet with the mixed powder and gently press it into a loose cake shape. The aperture of the microporous filter screen is 20 μm;

[0054] Step S2: Inject pH phosphate buffer solution with a preheating temperature of 80 °C as the first extraction liquid into the extraction chamber under pressure. The pressure is 15 MPa. Heat the extraction chamber to 90 °C, keep the pressure for 30 min, then open the liquid outlet of the microporous filter screen, inject air into the extraction chamber under pressure for 100 s, and then inject phosphate buffer solution into the extraction chamber under pressure for 40 s to obtain the first extraction liquid;

[0055] Step S3: Inject 30% ethanol aqueous solution as the second extraction liquid into the extraction chamber under pressure. The pressure is 25 MPa. Heat the extraction chamber to 82 °C, keep the pressure for 30 min, then open the liquid outlet of the microporous filter screen, inject air into the extraction chamber under pressure for 100 s, and then inject ethanol aqueous solution into the extraction chamber under pressure for 40 s to obtain the second extraction liquid;

[0056] Step S4: Mix the first extraction liquid, the second extraction liquid and the basic carrier, homogenize and adjust the pH to 5.5 - 6.0 to obtain the anti-allergy and soothing composition.

[0057] Comparative Example 1

[0058] Compared with Example 1, Comparative Example 1 does not contain Stephania tetrandra.

[0059] Comparative Example 2

[0060] Compared with Example 1, Comparative Example 2 does not contain Gentiana scabra.

[0061] Comparative Example 3

[0062] Compared with Example 1, Comparative Example 3 does not contain Paeonia lactiflora.

[0063] Comparative Example 4

[0064] Compared with Example 1, Comparative Example 4 does not contain Kochia scoparia.

[0065] Comparative Example 5

[0066] Compared with Example 1, Comparative Example 5 does not contain Perilla frutescens.

[0067] Comparative Example 6

[0068] Compared with Example 1, Comparative Example 6 does not contain the basic carrier.

[0069] Comparative Example 7

[0070] Compared with Example 1, Comparative Example 7 uses the traditional alcohol extraction method (reflux extraction with 70% ethanol) to replace Steps S1 to S3.

[0071] 1. Hyaluronidase inhibition test:

[0072] Test principle: Hyaluronidase is a participant in allergic reactions, and it has a strong correlation with inflammation and allergy. Many anti-allergy drugs act by inhibiting the activity of hyaluronidase. Therefore, testing the inhibition rate of hyaluronidase activity can characterize the anti-allergy effect of the sample.

[0073] Test method: Hyaluronidase hydrolyzes hyaluronic acid to produce β-N-acetylglucosamine. β-N-acetylglucosamine condenses with acetylacetone under alkaline conditions to form 2-methyl-3-diacetylpyrrole derivatives. The 2-methyl-3-diacetylpyrrole derivatives react with p-dimethylaminobenzaldehyde in a concentrated hydrochloric acid ethanol solution. The reactant has a characteristic absorption at 530 nm, and the change in absorbance value is linearly correlated with the degree of inhibition of hyaluronidase activity. By measuring the change in absorbance value, the hyaluronidase inhibition rate can be calculated, thereby analyzing the anti-allergy activity of the sample.

[0074] Take 0.1 mL of 0.25 mmol / L calcium chloride solution and 0.5 mL of hyaluronidase solution, incubate at 37 °C for 20 min; add 0.5 mL of plant composition, continue to incubate at 37 °C for 20 min; add 0.5 mL of sodium hyaluronate solution, incubate at 37 °C for 30 min, and let stand at room temperature for 5 min; add 0.1 mL of 0.4 mol / L sodium hydroxide solution and 0.5 mL of acetylacetone solution, heat in a boiling water bath for 15 min, and then immediately cool with ice water for 5 min; add 1.0 mL of Ehrlich reagent and dilute with 3.0 mL of absolute ethanol, let stand for 20 min for color development, and measure its absorbance value (ABS value) with a spectrophotometer. The calculation formula for the hyaluronidase inhibition rate is as follows:

[0075]

[0076] A is the ABS value of the control solution; B is the ABS value of the control blank solution; C is the ABS value of the sample solution; D is the ABS value of the sample blank solution. The hyaluronidase inhibition rates of Examples 1-3 and Comparative Examples 1-7 are shown in Table 1.

[0077] 2. Inhibitory test of cellular inflammatory factors:

[0078] Test principle: TNF-α is an inflammatory cytokine found in the tumor inflammatory microenvironment, mainly secreted by monocytes / macrophages, and can initiate chronic inflammatory responses. TNF-α regulates inflammatory responses by activating two inflammatory signaling pathways, namely nuclear transcription factor κB (NF-κB) and activator protein 1 (AP-1). It is an important inflammatory factor and is involved in the pathological damage of certain autoimmune diseases. Bacterial lipopolysaccharide (LPS) binds to the antigen recognition receptor on the surface of macrophages, inducing macrophages to secrete various cytokines such as TNF-α. TNF-α can activate three signaling pathways, namely Caspase protease, JNK, and transcription factor NF-κB, to achieve its biological functions such as cytotoxicity, antiviral, immunomodulation, and apoptosis, and is one of the important inflammatory factors.

[0079] Test method: LPS-induced RAW264.7 is a classic cell model for studying inflammatory factors. By comparing the differences in the content of TNF-α secreted by RAW264.7 after administration of the negative control and the test substance, the effect of the test substance on inhibiting TNF-α secretion is evaluated. The determination of the TNF-α content uses the enzyme-linked immunosorbent assay (ELISA). Specifically: Dilute macrophages with cell culture medium to the inoculation density (the confluence reaches 45%-60% 24 hours after inoculation), inoculate into a 96-well plate, with 200 μL of liquid in each well, and place it in a CO2 incubator for 24 ± 2 hours. Then discard the culture medium in the 96-well plate, add the culture medium containing a certain concentration of the sample and 1 μg / mL LPS to the wells, add the cell culture medium containing LPS to the negative control wells, and add the cell culture medium to the blank control wells, 200 μL in each well, and continue to culture in the CO2 incubator for 24 ± 2 hours. After the incubation culture is completed, collect 200 μL of the cell culture supernatant into a 1.5 mL sterile centrifuge tube and store it frozen in a -80°C ultra-low temperature refrigerator. According to the operation manual of the TNF-α ELISA detection kit, the samples are detected. The inhibition rates of cell inflammatory factors in Examples 1 to 3 and Comparative Examples 1 to 7 are shown in Table 1.

[0080] Table 1 Hyaluronidase inhibition rate and cell inflammatory factor inhibition rate

[0081] Sample Hyaluronidase inhibition rate (%) Cell inflammatory factor inhibition rate (%) Example 1 79.6 63.46 Example 2 77.1 62.75 Example 3 83.2 66.94 Comparative Example 1 22.6 15.38 Comparative Example 2 26.7 17.27 Comparative Example 3 27.4 18.98 Comparative Example 4 29.5 22.16 Comparative Example 5 32.9 26.13 Comparative Example 6 65.8 51.72 Comparative Example 7 56.3 42.27

[0082] As can be seen from Table 1, the hyaluronidase inhibition rates of Examples 1 to 3 are all greater than 77%, and the inhibition rates of cell inflammatory factors are all greater than 62%. Compared with Comparative Examples 1 to 5, Examples 1 to 3 using Stephania tetrandra, Gentiana scabra, Paeonia lactiflora, Kochia scoparia, and Perilla frutescens as plant active ingredients have a synergistic effect on the hyaluronidase inhibition rate and the cell inflammatory factor inhibition rate; compared with Comparative Example 6, adding a basic carrier in Examples 1 to 3 can effectively improve the anti-allergic effect of the composition; compared with Comparative Example 7, using the method of the present invention in Examples 1 to 3 can more effectively extract the active ingredients of plants.

[0083] 3. Volunteer test

[0084] Test subjects: Volunteers with discomfort problems such as facial skin flushing, itching, and stinging, a total of 100 people, 10 people in each group, and each sample is a group.

[0085] Test method: Prepare the samples obtained in Examples 1 to 3 and Comparative Examples 1 to 7 into a conventional emulsion at an addition amount of 3%; evenly apply the emulsion on the facial skin of the volunteers, once in the morning and once in the evening after washing the face with clean water, and observe the results after continuous use for 28 days. The statistical results of the effective personnel are shown in Table 2.

[0086] The validity statistical criteria are as follows: significant effect - the discomfort phenomenon completely disappears; obvious effect - the discomfort phenomenon significantly reduces; effective - the discomfort phenomenon reduces; ineffective - the discomfort phenomenon does not reduce or gets worse.

[0087] Table 2 Statistical Results Table of Valid Personnel

[0088] Significant effect Obvious effect Effective Ineffective Obvious effectiveness Example 1 9 1 0 0 100％ Example 2 9 1 0 0 100％ Example 3 10 0 0 0 100％ Comparative Example 1 0 1 3 6 10％ Comparative Example 2 0 1 4 5 10％ Comparative Example 3 0 1 5 4 10％ Comparative Example 4 0 2 4 4 10％ Comparative Example 5 0 2 5 3 10％ Comparative Example 6 6 2 2 0 80％ Comparative Example 7 5 2 3 0 70％

[0089] As can be seen from Table 2, the anti-allergic and soothing compositions prepared in Examples 1 to 3 of the present invention have excellent soothing effects compared to Comparative Examples 1 to 5, and also have better soothing effects compared to Comparative Examples 6 and 7.

[0090] The technical features of the above-described embodiments can be combined arbitrarily. For the sake of concise description, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, it should be considered as the scope described in this specification.

[0091] The above-described specific embodiments further elaborate on the purpose, technical solutions, and beneficial effects of the present invention. It should be understood that the above is only the specific embodiments of the present invention and is not used to limit the protection scope of the present invention. In particular, for those skilled in the art, any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention shall be included within the protection scope of the present invention.

Claims

1. An anti-allergy and soothing composition, characterized in that, The anti-allergy and soothing composition is composed of 15% - 30% of a base carrier and 70% - 85% of plant active ingredients by mass percentage. The plant active ingredients are composed of the following raw materials by mass parts: 15 - 30 parts of Stephania tetrandra, 5 - 15 parts of Gentiana scabra, 15 - 20 parts of Paeonia lactiflora, 10 - 15 parts of Kochia scoparia, 5 - 10 parts of Perilla frutescens; The preparation method of the anti-allergy and soothing composition includes the following steps: Step S1: After crushing, sieving and mixing Stephania tetrandra, Gentiana scabra, Paeonia lactiflora, Kochia scoparia and Perilla frutescens into a mixed powder, fill the mixed powder into an extraction chamber with a microporous filter screen outlet, and gently press it into a loose cake shape; Step S2: Pressurize and inject a phosphate buffer solution with a preheating temperature of 70°C - 80°C as the first extraction liquid into the extraction chamber, with a pressurization pressure of 10 MPa - 20 MPa, raise the temperature of the extraction chamber to 85°C - 92°C, and keep the pressure for 25 min - 40 min, and then pressurize and release the first extraction liquid to obtain the first extract; Step S3: Pressurize and inject an ethanol aqueous solution as the second extraction liquid into the extraction chamber, with a pressurization pressure of 15 MPa - 30 MPa, raise the temperature of the extraction chamber to 78°C - 85°C, and keep the pressure for 25 min - 40 min, and then pressurize and release the second extraction liquid to obtain the second extract; Step S4: Mix the first extract, the second extract and the base carrier, homogenize and adjust the pH to 5.5 - 6.0 to obtain the anti-allergy and soothing composition.

2. The anti-allergy and soothing composition according to claim 1, wherein The plant active ingredients are composed of the following raw materials by mass parts: 18 parts of Stephania tetrandra, 12 parts of Gentiana scabra, 20 parts of Paeonia lactiflora, 13 parts of Kochia scoparia, 8 parts of Perilla frutescens.

3. The anti-allergy and soothing composition according to claim 1, characterized in that, The base carrier includes one or more of ceramide, squalane, hyaluronic acid and panthenol.

4. The anti-allergy and soothing composition according to claim 3, characterized in that, The base carrier is composed of the following raw materials by mass ratio: panthenol:hyaluronic acid:squalane = 5:2:

8.

5. The anti-allergy and soothing composition according to claim 1, characterized in that, The particle size of the mixed powder is 300 mesh - 450 mesh.

6. The anti-allergy and soothing composition according to claim 1, characterized in that, The pore size of the microporous filter screen is 15 μm - 20 μm.

7. The anti-allergy and soothing composition according to claim 1, wherein In step S2, the pH of the phosphate buffer solution is 6.

0. Pressurize and release the first extraction liquid by opening the microporous filter screen outlet, and inject air into the extraction chamber under pressure for 30 s - 120 s, and then inject the phosphate buffer solution into the extraction chamber under pressure for 30 s - 120 s.

8. The anti-allergy and soothing composition according to claim 1, wherein In step S3, the concentration of the ethanol aqueous solution is 30%. Pressurize and release the second extraction liquid by opening the microporous filter screen outlet, and inject air into the extraction chamber under pressure for 30 s - 120 s, and then inject the ethanol aqueous solution into the extraction chamber under pressure for 30 s - 120 s.

9. Use of an anti-allergy and soothing composition according to any one of claims 1 to 8 in the preparation of an anti-allergy and soothing product, characterized in that, The anti-allergy and soothing product includes cream emulsion, gel, aqueous solution, essence or facial mask.

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