Toxicology experiment equipment and method for intelligently simulating water and sand environment

Abstract

The invention belongs to the technical field of toxicological experiments, and discloses toxicological experiment equipment for intelligently simulating a water and sand environment, which comprises a box body, an aquatic ecological area, an automatic liquid preparation device, a semi-permeable membrane living area, a layered net-shaped partition plate, a deposition area, a weighing sensor, a turbine suspension device and a computer, an SPME online detection device is arranged on the right side in the box body, the left side of the box body is connected with an automatic liquid preparation device, an aquatic ecological area is arranged in the middle of the box body, a semi-permeable membrane living area for isolating sand is arranged in the aquatic ecological area, a glass plate is arranged in the middle of the semi-permeable membrane living area, and an underwater camera is installed at the bottom of the glass plate. A layer net-shaped partition plate is arranged between the aquatic ecological area and the deposition area, an adjustable slope device is arranged on the right side of the deposition area, a weighing sensor is arranged at the bottom end of the deposition layer, the left side of the deposition layer is connected with a vacuum pump, and the two sides of the bottom of the box body are each provided with a turbine suspension stretching into the aquatic ecological area. And the computer controls the work of each device in the box body.

Description

Technical Field

[0001] The present invention belongs to the technical field of toxicological experiments, and particularly relates to a toxicological experiment device for intelligently simulating a water-sediment environment. Background Art

[0002] Currently, all standardized aquatic toxicological experiments are carried out in laboratories, which have a large difference from the water-sediment conditions in the natural environment, especially in the water-sediment conditions of sediment-laden waters. The results of toxicological experiments in the laboratory are often inconsistent with the toxicological effects of actual water bodies. Therefore, the present invention intends to develop an experimental device for simulating multi-sediment rivers such as the Yangtze River and the Yellow River in the laboratory. In the device, there are small devices that can isolate and culture model organisms such as chironomid larvae, daphnia magna, and zebrafish in the same water body, and can conduct toxicological experiments for comparative culture in two environments with and without sediment. It also includes a climate incubator function, which can provide conditions such as light, constant temperature, and maintaining oxygen levels. This can not only ensure that the water environment in the laboratory is close to the actual water body conditions but also ensure that the toxicological experiment process meets the national standard requirements. At the same time, it can specifically study the toxicological effects brought by sediment under the condition of ensuring the same concentration of water pollutants, and calculate the toxicity contribution of sediment-bound pollutants. In addition, a real-time monitoring system for detecting basic water condition indicators is set up, which can monitor indicators such as temperature, pH, conductivity, and dissolved oxygen content.

[0003] Through the above analysis, the problems and defects existing in the prior art are as follows:

[0004] (1) Currently, all standardized aquatic toxicological experiments are carried out in laboratories, which have a large difference from the water-sediment conditions in the natural environment, and the experimental results in the laboratory do not match the toxicological effects of actual water bodies.

[0005] (2) There is no device that combines a water-sediment condition simulation device with a toxicological experiment device. There is no device that can separate suspended sediment in the same water environment and ensure the same concentration of dissolved pollutants in the water. Summary of the Invention

[0006] In view of the problems existing in the prior art, the present invention provides a toxicological experiment device for intelligently simulating a water-sediment environment.

[0007] The present invention is implemented as follows. A toxicology experiment device for intelligently simulating a water-sediment environment includes: a box body, an aquatic ecological area, an automatic liquid dispenser, a semi-permeable membrane living area, a layer-shaped grid partition, a sedimentation area, a weighing sensor, a turbine suspension device, and a computer. An SPME on-line detection device is arranged on the right side inside the box body. The left side of the box body is connected to the automatic liquid dispenser. The middle part of the box body is set as the aquatic ecological area. Inside the aquatic ecological area, there is a semi-permeable membrane living area for isolating suspended sediment. A glass plate is arranged in the middle of the semi-permeable membrane living area. An underwater camera is installed at the bottom of the glass plate. A layer-shaped grid partition is arranged between the aquatic ecological area and the sedimentation area. An adjustable slope device is arranged on the right side of the sedimentation area. A weighing sensor is arranged at the bottom end of the sedimentation layer and is connected to a vacuum pump on the left side. On both sides of the bottom of the box body, there is a turbine suspension device extending into the aquatic ecological area. The box body is connected to the computer, and the computer controls the operation of each device inside the box body.

[0008] Furthermore, there are also provided:

[0009] 1. An AI intelligent grasping device:

[0010] An AI intelligent grasping device is installed on the box body. This device includes a high-precision robotic arm, an advanced visual recognition system, and an intelligent control unit.

[0011] The visual recognition system uses a high-resolution camera and combines deep learning algorithms to real-time identify biological samples and sediment conditions in the aquatic ecological area.

[0012] The intelligent control unit guides the robotic arm to perform precise sample grasping operations based on the visual recognition results.

[0013] 2. An integrated intelligent control system:

[0014] Integrate the AI intelligent grasping device with the existing computer control system to achieve unified intelligent management of the entire experimental device.

[0015] The control system adjusts the operation of the turbine suspension device, water flow speed and direction through real-time data analysis to simulate the natural water-sediment environment.

[0016] 3. Data analysis and feedback adjustment:

[0017] Experimental data (including water quality parameters, sediment conditions, biological sample conditions, etc.) are collected and analyzed in real time.

[0018] The control system automatically adjusts the experimental conditions according to the data analysis results to maintain the stability of the experimental environment and the healthy state of biological samples.

[0019] 4. Remote monitoring and operation:

[0020] Through a remote interface, researchers can remotely monitor the experimental process and remotely adjust the experimental settings when needed.

[0021] The real-time images and videos provided by the vision system help remote users understand the experimental situation more intuitively.

[0022] Application advantages:

[0023] Improve experimental accuracy: The AI intelligent grasping device can accurately grasp and manipulate samples, reducing human error.

[0024] Automated operation: The degree of automation in the experimental process is increased, reducing the manpower requirement and operation complexity.

[0025] Data-driven decision-making: Based on real-time data analysis, the experimental conditions are intelligently adjusted to ensure the effectiveness and accuracy of the experiment.

[0026] Remote operation and monitoring: It provides greater flexibility and convenience, especially in the case where long-term monitoring of the experiment is required.

[0027] Based on the above solutions, this intelligent toxicology experiment device for simulating the water-sediment environment with an AI intelligent grasping device will greatly improve the automation level and scientific research efficiency of the experiment.

[0028] Furthermore, the SPME device is used for on-line detection of the concentration of persistent pollutants; the aquatic ecological area is a living area for large aquatic independent model organisms and contains suspended sediment.

[0029] Furthermore, the semi-permeable membrane living area is composed of a semi-permeable membrane, which can block sediment from entering the semi-permeable membrane living area and is used as a comparison environment; the semi-permeable membrane living area is used to ensure that there are only differences in suspended sediment between the internal and external environments, and to study the toxicity effects of pollutants under the same other conditions.

[0030] Furthermore, the pore size of the layer-mesh partition can be replaced to separate benthic organisms and plankton; the vacuum pump can extract sediment samples in the sedimentation area or conduct sand and water drainage.

[0031] Furthermore, the underwater camera can take videos to observe and calculate the biological movement inhibition rate; it can also take photos for statistics of biological lethality.

[0032] Furthermore, the two turbine suspension devices can control the operation of a single turbine to form a small-slope sand bed and an upper-layer suspended sediment water area that can be restored; they can also operate the two turbines simultaneously to form a uniform suspended sediment water area.

[0033] Furthermore, the slope-adjustable device specifically includes:

[0034] The seepage separation tank is arranged at the central position inside the water tank. There is a water inlet forebay connected to the front end of the seepage separation tank. The water inlet end of the water inlet pipe is connected to the water supply tank, and the water outlet end of the water inlet pipe extends into the water inlet forebay. At the rear end of the water tank, there is a seepage outflow channel. At the rear end of the seepage separation tank, there is a river outflow channel, and the seepage outflow channel is arranged directly below the river outflow channel. The sediment settling bucket is arranged below the outlet of the river outflow channel, and the collection tank is arranged below the seepage outflow channel. The circulation pipeline connects the lower part of the collection tank with the drainage chamber. The front end of the bottom of the water tank is hinged to the front end of the second support frame, and a slope adjusting device is arranged at the rear end of the bottom of the water tank. Inside the experimental shed at the top of the water tank, there are a blower, a spray pipe, a lighting pipe, and an image collector.

[0035] Furthermore, the automatic liquid dispenser specifically includes: a container, which serves as a vessel for preparing the solution and holds the finally prepared solution; a volume determination device, which is located in the container, used to measure the volume of the added reagent, and input the detection result into the control unit; a liquid adding device, which includes multiple reagent bottles for holding reagents and the matching metering pumps, and can accurately add multiple groups of reagents into the container under the control of the control unit; a control unit, which is used to automate the operation of the solution preparation system; a delivery pipe, which is provided with a valve and can transport the prepared solution to the aquatic ecological area through the pipeline.

[0036] Furthermore, an underwater robot is used to track the growth state of aquatic organisms, capture suspended organisms or benthic organisms, and collect biological samples according to the toxicological experiment design. Specifically, it includes: a housing, inside which a drive shaft is installed through a bearing. One end of the drive shaft is fixedly connected to a drive cover. A drive angle adjustment mechanism is arranged inside the housing. A spindleless drive mechanism is arranged inside the drive cover. A cleaning mechanism is arranged on the housing. A camera cover is fixedly connected to the housing. A supplementary light is arranged on the outside of the housing. A support is fixedly connected to the housing. An end cover is fixedly connected to the outside of the housing. The other end of the drive shaft is fixedly connected to a first spur gear. A motor is fixedly connected inside the housing. A second spur gear is fixedly connected to the output shaft of the motor. The first spur gear meshes with the second spur gear. A robotic arm and a camera are arranged outside the housing. The robotic arm is used for collecting biological samples, and the camera is used for collecting images and monitoring the growth state of aquatic organisms.

[0037] Another object of the present invention is to provide a method for conducting a toxicity effect experiment using a toxicological experiment device that intelligently simulates the water and sediment environment, which is characterized by including the following steps:

[0038] Use an SPME online detection device to conduct real-time concentration monitoring of persistent pollutants in the aquatic ecological area;

[0039] Utilize a semi-permeable membrane living area to isolate suspended sediment to create experimental control groups under different environments;

[0040] Take videos and photos through an underwater camera to monitor and count the activity inhibition rate and lethality rate of organisms in the aquatic ecosystem;

[0041] Use a vacuum pump to extract sediment samples from the sediment area and analyze the distribution and concentration of pollutants in the sediment;

[0042] Control the turbine suspension device by computer to simulate the flow and deposition processes in the natural water-sediment environment and observe the responses of organisms to different environmental conditions.

[0043] Furthermore, it includes the following steps:

[0044] Use an AI intelligent grasping device to accurately grasp and manipulate biological samples in the aquatic ecosystem based on the recognition results of the vision system;

[0045] The vision system uses a deep learning algorithm to analyze the captured images to achieve efficient recognition of the characteristics of biological samples;

[0046] The intelligent control unit calculates the motion trajectory and operation strategy of the robotic arm based on the recognition results of the vision system to achieve accurate grasping and manipulation of biological samples;

[0047] Use the replaceable aperture of the layer mesh partition to adjust the separation degree of water and sediment according to experimental requirements;

[0048] Use a double turbine suspension device to form a uniform suspended sediment water area to simulate the sediment suspension state in the natural environment.

[0049] Combined with the above technical solutions and the solved technical problems, the advantages and positive effects of the technical solution to be protected by the present invention are as follows:

[0050] First, the present invention is also a climate incubator, which can take underwater photos, monitor in real time whether organisms survive and their movement conditions, and calculate the movement inhibition rate in real time, and conduct real-time monitoring of the water environment, including the detection of parameters such as water temperature, pH, conductivity, and dissolved oxygen content.

[0051] The present invention can perform inductive weighing, observe the increase and decrease of sediment, automatically prepare solutions, and control the concentration of water pollutants. And conduct on-line analysis and monitoring of the pollutant concentration of the SPME device, and adopt turbine and slope technologies to simulate the actual river bed.

[0052] The present invention can be used for simultaneous experiments on a variety of aquatic organisms. There is an ecological system with coexistence of benthic organisms and plankton, and food chain ecological analysis can be carried out. Benthic organisms feed on bottom mud, Daphnia magna feeds on nutrients in water, and fish feed on Daphnia magna.

[0053] Second, the technical solution provided by the present invention involves multiple technologies. The most core ones are the semi-permeable membrane isolation suspended sediment device, the AI intelligent monitoring and grabbing device, the online weighing system, and the SPME online detection system.

[0054] The present invention can significantly improve the equivalence between the toxicological experiment results in the laboratory and the toxicological effects in the natural environment outside the laboratory.

[0055] The present invention can greatly shorten the concentration detection time of pollutants in water bodies and significantly reduce the usage amount of toxic and harmful reagents.

[0056] The present invention can accurately grab the dead and inhibited aquatic animals, process the toxicological data in a timely manner, and there will be no misjudgment where an animal that is taken out as dead is actually found to be alive.

[0057] The present invention can control the concentration of water body pollutants in real time. For some volatile organic pollutants, during the toxicological experiment, due to factors such as volatilization or biodegradation, the concentration decreases. Through the online detection system and the solution configuration system, the pollutants can be replenished in real time to maintain the stability of the pollutant concentration in the water body and make the toxicological data more accurate.

[0058] The present invention can change the water and sediment conditions in real time. Through the monitoring of the sediment discharge port and the weighing sensor, the setting of the bottom sediment content can be achieved. By setting the rotation speeds of the two turbines, the content of suspended sediment can be changed, and the concentration change of suspended sediment corresponds to the setting of the turbine rotation speed. This is beneficial for the different water and sediment environmental conditions set in the experimental design.

[0059] Third, the technical solution of the present invention fills the technical gaps in the domestic and international industries:

[0060] There is no device that combines the water and sediment simulation environment and the aquatic toxicological experiment environment at home and abroad.

[0061] The technical solution of the present invention solves the technical problems that people have been eager to solve but have never succeeded in: the toxicological experiment results in the laboratory and the toxicological effects in the natural environment often have large differences. This device will make up for this deficiency. In the toxicological environment that meets national standards in the laboratory, it integrates the water and sediment environment under natural conditions, and at the same time adds intelligent tracking, online monitoring, and online detection systems to simulate and display the scenario of aquatic organisms under the natural environment in real time.

[0062] The technical solution of the present invention overcomes the technical prejudice: the personnel doing aquatic toxicological experiments have never been able to combine the toxicological experiment in the laboratory with the natural environment condition simulation device to complete the standardized toxicological experiment that is closest to the basin environment. This invention makes up for this defect.

[0063] Fourth, the toxicological experiment equipment implemented in the present invention has brought remarkable technological progress in the toxicological research of simulating the water-sediment environment, which is mainly reflected in the following aspects:

[0064] 1) Real-time pollutant monitoring:

[0065] The introduction of the SPME on-line detection device enables the concentration of persistent pollutants to be monitored on-line in real time, improving the timeliness and accuracy of data acquisition, and helping to more accurately evaluate the pollution status in the environment.

[0066] 2) High degree of simulation of the natural environment:

[0067] The combined use of the aquatic ecological area and the semi-permeable membrane living area in the equipment can simulate the real water-sediment environment, providing a more accurate experimental platform for studying the behavior and impact of pollutants in a similar natural environment.

[0068] 3) Precise environmental control:

[0069] The design of the semi-permeable membrane living area allows researchers to change only the suspended sediment conditions while keeping other conditions the same, so as to more accurately evaluate the impact of sediment on the toxic effects of pollutants.

[0070] 4) Intuitive observation of biological activities:

[0071] The installation of the underwater camera enables the behavior of organisms in the experimental environment to be directly observed and recorded, thus effectively evaluating the impact of pollutants on biological activities.

[0072] 5) Flexibility in sediment treatment:

[0073] The application of the layer mesh partition board and the vacuum pump makes the treatment of sediment (including sampling or sediment and water drainage) more flexible and efficient, helping to more comprehensively understand the behavior of pollutants in the sediment environment.

[0074] 6) Comprehensive data analysis ability:

[0075] Through connection with the computer, experimental data can be collected and processed, providing data support for in-depth analysis of the toxic effects of pollutants.

[0076] The design and function of the toxicological experiment equipment provided by the present invention provide a highly integrated experimental platform that simulates the natural environment, which is of great significance for research in the fields of environmental science and ecological toxicology, especially in the research on evaluating the survival risk and toxic effects of pollutants on aquatic organisms. Description of the Drawings

[0077] To more clearly illustrate the technical solutions of the embodiments of the present invention, the following will briefly introduce the drawings required for use in the embodiments of the present invention. Obviously, the following described drawings are only some embodiments of the present invention. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts.

[0078] Figure 1 It is a structural diagram of a toxicological experiment device for an intelligent simulated water and sediment environment provided by an embodiment of the present invention;

[0079] Figure 2 It is a schematic diagram of the shooting effect provided by an embodiment of the present invention;

[0080] Figure 3 It is a schematic diagram of an SPME on-line detection device provided by an embodiment of the present invention;

[0081] Figure 4 It is a schematic diagram of a sampling device of an SPME on-line detection device provided by an embodiment of the present invention;

[0082] Figure 5 It is a schematic diagram of a turbine suspension device provided by an embodiment of the present invention;

[0083] Figure 6 It is a schematic diagram of an adjustable slope device provided by an embodiment of the present invention;

[0084] Figure 7 It is a schematic diagram of an automatic liquid dispenser provided by an embodiment of the present invention;

[0085] Figure 8 It is a structural diagram of an underwater robot provided by an embodiment of the present invention;

[0086] Figure 9 It is a schematic diagram of the semi-permeable membrane principle of the semi-permeable membrane living area provided by an embodiment of the present invention;

[0087] In the figure: 1. Box body; 2. SPME on-line detection device; 3. Aquatic ecological area; 4. Underwater camera; 5. Computer; 6. Adjustable slope device; 7. Weighing sensor; 8. Deposition area; 9. Vacuum pump; 10: Layered mesh partition; 11. Turbine suspension device; 12. Glass plate; 13. Semi-permeable membrane living area; 14. Automatic liquid dispenser; 15. Bed base; 16. SPME sampling needle; 17. Pollutant bottle; 18. Solvent barrel; 19. Quantitative liquid sampler; 20. Mixing device; 21. AI camera; 22. Automatic grasping arm. Specific embodiments

[0088] In order to make the objectives, technical solutions and advantages of the present invention more clear and understandable, the present invention will be further described in detail below in conjunction with embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0089] Aiming at the problems existing in the prior art, the present invention provides a toxicological experiment device for intelligently simulating a water-sediment environment. The present invention will be described in detail below in conjunction with the accompanying drawings.

[0090] As Figure 1 shown, the toxicological experiment device for intelligently simulating a water-sediment environment provided by an embodiment of the present invention includes: a box body 1, an aquatic ecological area 3, an automatic liquid dispenser 14, a semi-permeable membrane living area 13, a layer net-shaped partition 10, a sedimentation area 8, a weighing sensor 7, a turbine suspension device 11, and a computer 5; an SPME on-line detection device 2 is arranged on the right side inside the box body 1, the left side of the box body 1 is connected to the automatic liquid dispenser 14, the middle part of the box body 1 is set as the aquatic ecological area 3, a semi-permeable membrane living area 13 for isolating sediment is arranged inside the aquatic ecological area 3, a glass plate 12 is arranged in the middle of the semi-permeable membrane living area 13, an underwater camera 4 is installed at the bottom of the glass plate 12, a layer net-shaped partition 10 is arranged between the aquatic ecological area 3 and the sedimentation area 8, an adjustable slope device 6 is arranged on the right side of the sedimentation area 8, a weighing sensor 7 is arranged at the bottom end of the sediment layer and is connected to a vacuum pump 9 on the left side, a turbine suspension device 11 extending into the aquatic ecological area 3 is arranged on each side at the bottom of the box body 1, and the box body 1 is connected to the computer 5, and the computer 5 controls the operation of each device inside the box body 1.

[0091] The SPME device 2 is used for on-line detection of the concentration of persistent pollutants; the aquatic ecological area 3 is a living area for large aquatic independent mode organisms and contains suspended sediment.

[0092] The semi-permeable membrane living area 13 is composed of a semi-permeable membrane. The semi-permeable membrane can block sediment from entering the semi-permeable membrane living area 13 and is used as a comparison environment; the semi-permeable membrane living area 13 is used to ensure that there are only differences in suspended sediment between the internal and external environments, and to study the toxic effects of pollutants under the condition that other conditions are the same.

[0093] The layer net-shaped partition 10 has replaceable pore sizes to separate water and sediment; the vacuum pump 9 can extract sediment samples in the sedimentation area 8 or drain sand and water.

[0094] The underwater camera can take videos to observe the biological activity inhibition rate; it can take photos to count the biological lethality rate, Figure 2 which is a schematic diagram of the shooting effect.

[0095] Based on the described toxicological experiment device for intelligently simulating a water-sediment environment, introducing an AI intelligent grasping device can further improve the automation degree and accuracy of the experiment. The following is a new scheme including the AI intelligent grasping device:

[0096] 1. AI Intelligent Grabbing Device:

[0097] Install the AI intelligent grabbing device at a suitable position in the box. This device includes a high-precision robotic arm, an advanced visual recognition system, and an intelligent control unit.

[0098] The visual recognition system uses a high-resolution camera and combines deep learning algorithms to identify biological samples and sediment conditions in the aquatic ecological area in real time.

[0099] The intelligent control unit guides the robotic arm to perform precise sample grabbing operations based on the visual recognition results.

[0100] 2. Integrated Intelligent Control System:

[0101] Integrate the AI intelligent grabbing device with the existing computer control system to achieve unified intelligent management of the entire experimental equipment.

[0102] The control system adjusts the operation of the turbine suspension device, water flow speed, and direction through real-time data analysis to simulate the natural water and sediment environment.

[0103] 3. Data Analysis and Feedback Adjustment:

[0104] Experimental data (including water quality parameters, sediment conditions, biological sample conditions, etc.) are collected and analyzed in real time.

[0105] The control system automatically adjusts the experimental conditions according to the data analysis results to maintain the stability of the experimental environment and the healthy state of biological samples.

[0106] 4. Remote Monitoring and Operation:

[0107] Through the remote interface, researchers can remotely monitor the experimental process and remotely adjust the experimental settings when needed.

[0108] The real-time images and videos provided by the visual system help remote users more intuitively understand the experimental situation.

[0109] Application Advantages:

[0110] Improve experimental accuracy: The AI intelligent grabbing device can precisely grab and manipulate samples, reducing human errors.

[0111] Automated operation: The degree of automation in the experimental process is increased, reducing the manpower requirements and operation complexity.

[0112] Data-driven decision-making: Based on real-time data analysis, the experimental conditions are intelligently adjusted to ensure the effectiveness and accuracy of the experiment.

[0113] Remote operation and monitoring: It provides greater flexibility and convenience, especially in cases where long-term monitoring of experiments is required.

[0114] The toxicology experiment equipment for intelligent simulation of water and sediment environment containing AI intelligent capture device will greatly improve the automation level and scientific research efficiency of the experiment.

[0115] The two turbine suspension devices 11 can control the operation of a single turbine to form a sloping sand bed and an upper suspended sediment water area; or the two turbines can operate simultaneously to form a uniform suspended sediment water area. The slope adjustment device specifically includes:

[0116] The seepage separation tank is arranged at the central position in the water tank. A water inlet forebay connected to it is provided at the front end of the seepage separation tank. The water inlet end of the water inlet pipe is connected to the water supply tank, and the water outlet end of the water inlet pipe extends into the water inlet forebay; a seepage outlet channel is arranged at the rear end of the water tank, and a river outlet channel is provided at the rear end of the seepage separation tank. The seepage outlet channel is arranged directly below the river outlet channel; a sediment bucket is arranged below the outlet of the river outlet channel, and a collection tank is arranged below the seepage outlet channel; a circulation pipeline connects the lower part of the collection tank to the drainage chamber; the front end of the bottom of the water tank is hinged to the front end of the second support frame, and a slope adjustment device is provided at the rear end of the bottom of the water tank; inside the experimental shed at the top of the water tank, there are a blower, a spray pipe, a lighting pipe, and an image collector.

[0117] The automatic liquid dispenser 14 specifically includes: a container, which serves as a vessel for preparing the solution and holds the finally prepared solution; a volume fixing device, located in the container, used to measure the volume of the added reagent and input the detection result into the control unit; a liquid adding device, including multiple reagent bottles for holding reagents and the supporting metering pumps, which can accurately add multiple groups of reagents into the container under the control of the control unit; a control unit, used to automate the operation of the solution preparation system; a delivery pipe, provided with a valve, which can transport the prepared solution to the aquatic ecological area 3 through the pipeline.

[0118] An underwater robot is used to track the growth status of aquatic organisms, capture suspended organisms or benthic organisms, and collect biological samples according to the toxicology experiment design. Specifically, it includes: a housing, inside which a drive shaft is installed through a bearing. One end of the drive shaft is fixedly connected to a drive cover. A drive angle adjustment mechanism is arranged inside the housing. A spindleless drive mechanism is arranged inside the drive cover. A cleaning mechanism is arranged on the housing. A camera cover is fixedly connected to the housing. A supplementary light is arranged on the outside of the housing. A support is fixedly connected to the housing. An end cover is fixedly connected to the outside of the housing. The other end of the drive shaft is fixedly connected to a first spur gear. A motor is fixedly connected inside the housing. A second spur gear is fixedly connected to the output shaft of the motor. The first spur gear meshes with the second spur gear. A robotic arm and a camera are arranged outside the housing. The robotic arm is used for collecting biological samples, and the camera is used for collecting images and monitoring the growth status of aquatic organisms.

[0119] Preferably, the toxicological experiment device for intelligently simulating the water and sediment environment provided by the embodiment of the present invention is an overall biochemical incubator, which has the functions of a biochemical incubator and can provide conditions such as illumination and constant temperature.

[0120] There is a glass water tank inside, and the water tank simulates the natural state of sediment rivers such as the Yellow River and the Yangtze River through turbine stirring.

[0121] In the entire water environment, an aquatic organism culture device is set. Benthic organisms (such as chironomid larvae) can be cultured in the sediment, and the suspended organism culture device in the water body is composed of a stainless steel frame covered with a semi-permeable membrane. In the semi-permeable membrane device, model organisms such as daphnia magna and zebrafish are standardized cultured, and toxicological experiments with two environments of with sediment and without sediment can be carried out. In this way, it can not only ensure that the water environment is close to the reality, but also ensure that the growth environment of the toxicological model organisms meets the requirements of the national standard for the toxicological experiment environment.

[0122] The organisms in the two environments of with sediment and without sediment can have the same temperature and pollutant concentration, and it is very easy to calculate the different toxicological contributions brought by the sediment through targeted research.

[0123] At the same time, real-time monitoring of the basic detection indicators of the water body is also set, including temperature, pH, conductivity, dissolved oxygen content, etc.

[0124] A waterproof microscopic photographing machine is set in the internal device, which can take pictures of the survival situation of daphnia magna and calculate the movement inhibition rate. The movement inhibition rate of daphnia magna refers to the loss of activity ability within 15 s. Based on this, 24hLC50 or 48hEC50 can be calculated. It can be externally connected to a computer for real-time monitoring and counting.

[0125] The toxicological experiment device of the present invention is mainly used to simulate the water and sediment environment and study the toxic effects of pollutants in a similar natural environment. The following is the detailed working principle of the device:

[0126] 1. Setting of the box body and components

[0127] Box body 1: As the main structure of the entire experimental device, it contains multiple functional areas inside.

[0128] SPME on-line detection device 2: Installed on the right side of the box body, used for on-line detection of the concentration of persistent pollutants.

[0129] Automatic dispenser 14: Located on the left side of the box body, responsible for automatically dispensing the liquids required for the experiment.

[0130] Aquatic ecological area 3: Located in the middle of the box body, it is a large aquatic independent simulated biological living area, containing suspended sediment, and is used to simulate the natural water environment.

[0131] Semi-permeable membrane living area 13: Composed of semi-permeable membrane, located inside the aquatic ecological area, blocking sediment, serving as a comparison environment.

[0132] Glass plate 12 and underwater camera 4: The camera is located at the bottom of the glass plate, used to take videos and photos, observe and record biological activities.

[0133] Layered mesh partition 10: Located between the aquatic ecological area and the sedimentation area, with replaceable pore sizes, used to separate water and sediment.

[0134] Sedimentation area 8 and adjustable slope device 6: Used to simulate the sedimentation environment, and the adjustable slope device is used to adjust the slope of the sediment layer.

[0135] Load cell 7: Located at the bottom end of the sediment layer, used to measure the weight of the entire water environment. Since the weighing device is at the bottom, the mass of the entire system is measured, and then the sediment mass, suspended sediment concentration, pollutant concentration, etc. are calculated based on information such as the initial added sediment mass and water volume.

[0136] Vacuum pump 9: Located on the left side of the sedimentation area, used to extract sediment samples or remove excess sediment and water.

[0137] 2. Working principle

[0138] Pollutant concentration monitoring:

[0139] The SPME on-line detection device continuously monitors the concentration of pollutants in the water body to evaluate the pollution status in the water environment.

[0140] Ecological area simulation and comparison experiment:

[0141] The aquatic ecological area simulates the natural water environment, providing an almost natural living space for aquatic organisms.

[0142] The semi-permeable membrane living area serves as a comparison environment to ensure that other conditions are the same as those in the aquatic ecological area except for suspended sediment, so as to study the impact of sediment on the toxic effects of pollutants.

[0143] Observation of biological activities:

[0144] The underwater camera records the activities of organisms under different conditions. The videos can be used to observe the survival status of organisms, and the photos and videos are used to count the biological activity inhibition rate and biological lethality rate.

[0145] Sediment treatment:

[0146] The layered mesh partition is used for physically isolating water and sediment, and the vacuum pump can be used to extract samples in the sedimentation area or remove sediment and water.

[0147] The load cell monitors the weight change of the sediment.

[0148] Data processing and analysis: All monitored and recorded data are collected and processed by computer 5 for analyzing the toxic effects of pollutants and the physiological responses of organisms.

[0149] Through this device, the behavior of pollutants in the water-sediment environment and their impacts on aquatic organisms can be more accurately simulated and studied, providing a scientific basis for environmental protection and pollution control.

[0150] Product performance features:

[0151] 1. Microcomputer automatic control; LCD display, touch switch, easy to operate (optional touch screen display).

[0152] 2. Programmable control mode; 30 groups of multi-segment programming parameter values can be set (light intensity control range: 0 - 100%, unit 100 levels adjustable).

[0153] 3. Equal light intensity control technology per unit area to ensure uniform light exposure of the culture.

[0154] 4. With power failure memory function, when powered on again, it continues to run from the power-off point according to the original set value; has continuous operation and timing functions.

[0155] 5. Intelligent long-term refrigeration anti-icing technology and PID heating technology; taking into account the accuracy of temperature and humidity control, as well as energy conservation and environmental protection.

[0156] 6. With over-temperature, under-temperature or sensor abnormality protection function to ensure the safety of experimental samples and instruments.

[0157] 7. Advanced circulating air duct design, duct-type ventilation structure and equal air return technology.

[0158] 8. The air speed in the working chamber is gentle, and the temperature and humidity are uniform. The air speed of the circulating fan is adjustable optionally.

[0159] 9. Adopts Springspectrum full-spectrum cold light source for plant lighting; ensures efficient light source supply.

[0160] 10. Stainless steel inner tank; adjustable shelf height; plastic-sprayed anti-static cold-rolled steel plate shell.

[0161] 11. Adopts Jiangnan patented core control system, and core components all use well-known brands.

[0162] 12. Selects well-known brand compressors and circulating fans, and cooperates with strict and professional refrigeration technology to ensure the long-term reliability of the system quality, making users worry-free.

[0163] The extraction process of the SPME sampling probe is as follows: the incubation time before extraction is 2 minutes, the incubation temperature is 60 °C; the incubation oscillation speed is 250 revolutions per minute; the aging temperature of the extraction head is 270 °C, and the aging time is 5 minutes; the extraction time is 30 minutes; the desorption time is 5 minutes.

[0164] The load cell can quickly and safely convert storage tanks, hoppers, and conveyors into scales. Built-in functions ensure the maintenance of safety, performance, and accuracy.

[0165] Schematic diagram of the semi-permeable membrane in the semi-permeable membrane living area, which can ensure that there is no sediment inside the membrane and there is sediment outside the membrane.

[0166] Example 1 - Artificial climate incubator with a water-sand environment

[0167] The present invention provides an incubator with a simulated water-sand environment. Other incubators can only be used as toolboxes for single breeding functions to carry out standardized breeding of model organisms, and cannot conduct toxicological experiments on aquatic animals, let alone simulate the natural water-sand environment. This invention moves the entire water-sand simulation environment into the laboratory and sets it as a large toxicological experiment system as a whole, combining the natural water-sand conditions outside the laboratory with the toxicological experiments inside the laboratory into a system, and can predict the survival status of aquatic model organisms in the wild environment inside the laboratory. This is the most important innovation point.

[0168] Example 2 - AI intelligent object-taking robot

[0169] The present invention provides an intelligent device that can obtain the target organism at any time. There is a miniature AI grabbing device in the water body, which is equipped with an intelligent monitoring system (camera) and is connected to an external computer. It can be used alone in other scenarios or as one of the modules in the present invention. The device has a 360° rotating camera underwater, which can track the survival movement state of aquatic organisms and grab suspended organisms or benthic organisms to complete the collection of biological samples.

[0170] Example 3 - Automatic liquid dispenser

[0171] The present invention provides a device for automatically preparing solutions that can be connected to a water-sand simulation box. It has an automatic dilution function. After filling the dilution solvent, the standard sample of the target pollutant is diluted according to the dilution ratio calculated by the computer, and the diluted pollutant solution is pushed into the water tank of the device of the present invention through a syringe-like push rod.

[0172] Example 4 - SPME passive sampling on-line detector:

[0173] The present invention provides an SPME sampling film or an SPME sampling probe, which can be connected to a gas chromatography-mass spectrometer or other large-scale detection instruments for use, so as to realize the on-line detection of various water pollutants such as polycyclic aromatic hydrocarbons and perfluorinated compounds. It can also be used alone as a sampler. Only a very small amount of water sample in the microliter level needs to be consumed, and then it can be taken out for the detection of water pollutants.

[0174] Example 5 - Underwater camera in sediment-free environment:

[0175] The present invention provides a device specifically for photographing sediment-free environments. It is specifically used for photographing the environment and organisms inside the semi-permeable membrane. The toxicological index: inhibition rate of movement can be calculated according to the movement track of Daphnia magna.

[0176] Example 6 Inductive weighing equipment

[0177] The present invention provides for grasping the weight of the entire water-sediment system through sensors. When water samples need to be taken out, aquatic animals need to be captured, and sediment needs to be discharged, weighing can be carried out in real time to master the sampling amount and used to calculate the amount of pollutant standard solution, aqueous solution, sediment, etc. that need to be supplemented.

[0178] For the study of the toxicity of hot-spot pollutants such as heavy metals, persistent organic pollutants, and new pollutants in water to aquatic organisms under the water-sediment environment, the entire device provides an environmental condition that can meet the requirements of standard toxicological experiments and an experimental condition close to the actual water-sediment environment. In the past, the results of toxicological experiments in laboratories often did not conform to the toxicological effects in the actual environment, and this device will maximize the compensation for this deficiency.

[0179] Application Example 1: Study on the impact of river pollutants

[0180] 1) Box setting:

[0181] An aquatic ecological area simulating the river environment is established, including natural water flow and suspended sediment.

[0182] An SPME on-line detection device is installed in the box to monitor the pollutant concentration in the water body, such as heavy metals or organic pollutants.

[0183] 2) Experimental operation:

[0184] Pollutants with a specific concentration are injected into the aquatic ecological area.

[0185] The sediment is isolated using the semi-permeable membrane living area to create a control group to study the influence of bottom sediment and suspended sediment on the toxic effect of pollutants.

[0186] An underwater camera is used to record the survival activities of aquatic organisms in different environments.

[0187] 3) Data collection and analysis:

[0188] Monitor the change of pollutant concentration in real time.

[0189] Observe and record the behavioral differences of aquatic organisms under the conditions of with and without sediment, and evaluate the biological effects of pollutants.

[0190] Use a computer to perform data analysis and evaluate the ecological toxicity of pollutants.

[0191] Application Example 2: Evaluation of the impact of industrial wastewater discharge

[0192] 1) Box configuration:

[0193] Set up an aquatic ecological area to simulate the water environment near the industrial area, including the suspended particulate matter commonly found in industrial wastewater.

[0194] Install an SPME on-line detection device to monitor the concentration of specific chemical pollutants, such as benzene, phenolic compounds, etc.

[0195] 2) Experimental operation:

[0196] Simulate the injection of industrial wastewater into the aquatic ecological area to create an experimental group.

[0197] Use a semi-permeable membrane living area to isolate the solid particles in industrial wastewater to study their contribution to the toxicity of pollutants.

[0198] Use an underwater camera to monitor and record the physiological reactions of organisms in a polluted environment.

[0199] 2) Experimental operation:

[0200] Simulate the injection of a pollutant solution with a known concentration automatically configured into the aquatic ecological area to create an experimental group.

[0201] Use a semi-permeable membrane living area to isolate the solid particles in the water body containing pollutants at a specific concentration to study their contribution to the toxicity of pollutants.

[0202] Use an underwater camera to monitor and record the physiological reactions of organisms in a polluted environment.

[0203] 3) Sample collection and processing:

[0204] Collect sediment samples in the deposition area through a layer of mesh partition and a vacuum pump.

[0205] Analyze the concentration and type of pollutants in the sediment samples.

[0206] 4) Data analysis:

[0207] Track and record the change of pollutant concentration in real time.

[0208] Compare the behaviors and physiological responses of organisms with and without sediment to evaluate the impact of industrial wastewater on the aquatic ecosystem.

[0209] Use a computer for data processing and toxicological effect analysis.

[0210] From these two embodiments, it can be seen that the toxicological experimental equipment of the present invention can be widely applied to the ecological toxicity research of various water environment pollutants, including river pollution and industrial wastewater discharge. The multifunctional design of the equipment and its precise data monitoring ability make it an effective tool for evaluating the impact of water environmental pollution.

[0211] In the description of the present invention, unless otherwise specified, "a plurality of" means two or more; the orientation or positional relationship indicated by terms such as "upper", "lower", "left", "right", "inner", "outer", "front end", "rear end", "head", "tail", etc. is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be construed as a limitation of the present invention. In addition, terms such as "first", "second", "third", etc. are only used for descriptive purposes and cannot be construed as indicating or implying relative importance.

[0212] The above is only the specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present invention, any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention shall be covered by the protection scope of the present invention.

Claims

1. An intelligent toxicological experiment method for simulating water and sediment environment, characterized by comprising the following steps: Use an SPME online detection device to monitor the real-time concentration of persistent pollutants in the aquatic ecological area; Isolate suspended sediment using a semi-permeable membrane living area to create experimental control groups under different environments; Monitor and count the activity inhibition rate and lethality of organisms in the aquatic ecological area by taking videos and photos with an underwater camera; Use a vacuum pump to extract sediment samples from the sediment area and analyze the distribution and concentration of pollutants in the sediment; Control the turbine suspension mixer through a computer to simulate the flow and deposition processes in the natural water and sediment environment, and observe the responses of organisms to different environmental conditions.

2. The method according to claim 1, wherein An SPME online detection device is arranged on the right side inside the box body. The left side of the box body is connected to an automatic liquid dispenser. The middle part of the box body is set as an aquatic ecological area. Inside the aquatic ecological area, there is a semi-permeable membrane living area for isolating sand. A glass plate is arranged in the middle of the semi-permeable membrane living area. An underwater camera is installed at the bottom of the glass plate. A layer of mesh partition is arranged between the aquatic ecological area and the sediment area. An adjustable slope device is arranged on the right side of the sediment area. A weighing sensor is arranged at the bottom end of the sediment layer and is connected to the vacuum pump on the left side. On both sides of the bottom of the box body, there is a turbine suspension mixer extending into the aquatic ecological area. The box body is connected to a computer, and the computer controls the operation of each device inside the box body.

3. The method according to claim 1, wherein An AI intelligent grasping device, including a high-precision robotic arm, a vision recognition system, and an intelligent control unit, is arranged in the box body and is used to accurately grasp and manipulate biological samples in the aquatic ecological area according to the recognition results of the vision system; Among them, the image processing module of the vision system uses a deep learning algorithm to analyze the captured images to identify the characteristics of biological samples; the intelligent control unit of the AI intelligent grasping device calculates the movement trajectory and operation strategy of the robotic arm based on the recognition results of the vision system to achieve accurate grasping and manipulation of biological samples.

4. The method according to claim 1, wherein The SPME online detection device is used for online detection of the concentration of persistent pollutants; the aquatic ecological area is a large aquatic independent mode biological living area containing suspended sediment; The semi-permeable membrane living area is composed of a semi-permeable membrane. The semi-permeable membrane can block the entry of sediment into the semi-permeable membrane living area and is used as a comparison environment; the semi-permeable membrane living area is used to ensure that there are only differences in suspended sediment between the internal and external environments, and to study the toxicity effects of pollutants under the condition that other conditions are the same.

5. The method according to claim 1, characterized in that, The layer of mesh partition can replace the pore size to separate water and sediment; the vacuum pump can extract sediment samples from the sediment area or drain sand and water; the underwater camera can take videos to observe the activity inhibition rate of organisms; it can take photos to count the lethality of organisms; the two turbine suspension mixers can control the operation of a single turbine to form a sloping sand bed and an upper suspended sediment water area; or the two turbines can operate simultaneously to form a uniform suspended sediment water area.

6. The method according to claim 1, wherein The adjustable slope device specifically includes: The seepage separation tank is arranged at the central position in the water tank. An inlet forebay connected to it is provided at the front end of the seepage separation tank. The water inlet end of the water inlet pipe is connected to the water supply tank, and the water outlet end of the water inlet pipe extends into the inlet forebay. A seepage outlet channel is arranged at the rear end of the water tank, and a river outlet channel is provided at the rear end of the seepage separation tank. The seepage outlet channel is arranged directly below the river outlet channel. The sediment passing bucket is arranged below the outlet of the river outlet channel, and the collection tank is arranged below the seepage outlet channel. The circulation pipeline connects the lower part of the collection tank with the drainage chamber. The front end of the bottom of the water tank is hinged to the front end of the second support frame, and a slope adjustment device is provided at the rear end of the bottom of the water tank. Inside the experimental shed at the top of the water tank, there are a blower, a spray pipe, a lighting pipe, and an image collector.

7. The method according to claim 1, wherein The automatic liquid dispenser specifically includes: a container, which serves as a utensil for preparing the solution and holds the finally prepared solution; a volume fixing device, which is located in the container, used to measure the volume of the added reagent and input the detection result into the control unit; a liquid adding device, which includes multiple reagent bottles for holding reagents and the supporting metering pumps, and can accurately add multiple groups of reagents into the container under the control of the control unit; a control unit, which is used to automate the operation of the solution preparation system; a delivery pipe, which is provided with a valve and can transport the prepared solution to the aquatic ecosystem area through the pipeline.

8. The method according to claim 1, characterized in that An underwater robot is used to track the growth status of aquatic organisms, capture suspended organisms or benthic organisms, and collect biological samples according to the toxicological experiment design. Specifically, it includes: a housing, inside which a drive shaft is installed through a bearing. One end of the drive shaft is fixedly connected to a drive cover. A drive angle adjustment mechanism is arranged inside the housing. A spindleless drive mechanism is arranged inside the drive cover. A cleaning mechanism is arranged on the housing. A camera cover is fixedly connected to the housing. A supplementary light is arranged on the outside of the housing. A support is fixedly connected to the housing.

9. The method according to claim 8, characterized in that, An end cover is fixedly connected to the outside of the housing. The other end of the drive shaft is fixedly connected to a first spur gear. A motor is fixedly connected inside the housing. A second spur gear is fixedly connected to the output shaft of the motor. The first spur gear meshes with the second spur gear. A robotic arm and a camera are arranged outside the housing. The robotic arm is used to collect biological samples, and the camera is used to collect images and monitor the survival status of aquatic organisms.

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