Anti-aging agilawood composition and preparation method thereof

Abstract

The invention discloses an anti-aging agilawood composition and a preparation method thereof, and belongs to the technical field of compositions. Comprising the following steps: taking agilawood powder, sequentially carrying out enzymolysis on the agilawood powder through cellulase, pectinase and alkaline protease, and collecting a product to obtain agilawood essential oil; taking the agilawood essential oil, the Terminalia arjuna extract, the pistachio seed oil, the benzoin resin extract, the rhinacanthus nasutus extract, the spirulina platensis extract, the aloe barbadensis flower extract, the sanguinea villosa flower extract, the stachyurus sikokii flower extract, the acanthopanax senticosus extract, deionized water and absolute ethyl alcohol; the agilawood composition is prepared by mixing the agilawood and the disodium lauryl polyoxyethylene ether sulfosuccinate, and uniformly stirring the mixture, so that the anti-aging agilawood composition is obtained. And the anti-aging effect is achieved through compatibility and synergism of multiple components.

Description

Technical Field

[0001] The present invention belongs to the technical field of compositions, and particularly relates to an anti-aging agarwood composition and a preparation method thereof. Background Art

[0002] Skin aging is a complex physiological process, which is a skin aging phenomenon caused by the combined action of various natural and non-natural factors. This phenomenon is mainly manifested in the skin as dry skin, a gradual decline in the barrier function, rough skin, a decrease in elastic fibers resulting in reduced skin elasticity, the appearance of wrinkles, and irregular pigmentation. The reasons for skin aging are manifold. In addition to the inevitable natural aging process, they also include external environmental factors such as long-term exposure to ultraviolet rays, lack of skin nutrition, excessive production of oxygen free radicals, internal emotional stress, hormonal imbalance, and insufficient sleep due to long-term staying up late. With the development of society and the improvement of people's living standards, the awareness of skin care has gradually increased, and more and more people have begun to pay attention to the problems of "anti-aging" and "anti-initial aging", and the demand for anti-aging products has shown a significant growth trend. Therefore, in order to meet the market demand, the research on delaying skin aging has become increasingly important. At present, developing an anti-aging composition that can comprehensively promote skin repair and effectively slow down the aging process has become an urgent technical problem to be solved. Summary of the Invention

[0003] The purpose of the present invention is to provide an anti-aging agarwood composition and a preparation method thereof, which are used to solve the problems of skin aging, promoting skin repair, and slowing down the aging process.

[0004] The purpose of the present invention can be achieved through the following technical solutions: A preparation method of an anti-aging agarwood composition includes the following steps: Take agarwood powder and enzymatically hydrolyze it successively with cellulase, pectinase, and alkaline protease, and collect the product to obtain agarwood essential oil; Take the agarwood essential oil, Terminalia arjuna extract, Pistacia vera seed oil, Styrax benzoin resin extract, Rhinacanthus nasutus extract, Spirulina platensis extract, Aloe barbadensis Miller flower extract, Abelmoschus manihot flower extract, Stachyurus himalaicus extract, Acanthopanax senticosus extract, deionized water, anhydrous ethanol, and disodium laureth sulfosuccinate, mix them and stir evenly to obtain the anti-aging agarwood composition.

[0005] As a preferred technical solution of the present invention, the mass ratio of the agarwood essential oil, Terminalia arjuna extract, pistachio seed oil, benzoin resin extract, Rhinacanthus nasutus extract, Spirulina platensis extract, Aloe vera flower extract, Calotropis procera flower extract, Stachyurus himalaicus extract, Eleutherococcus senticosus extract, deionized water, absolute ethanol, and disodium laureth sulfosuccinate is 5-10: 0.7-0.9: 0.3-0.5: 0.4-0.6: 0.2-0.3: 0.5-0.7: 0.4-0.6: 0.5-0.9: 0.1-0.3: 0.2-0.5: 20-30: 15-25: 0.004-0.006.

[0006] As a preferred technical solution of the present invention, the dosage ratio of the cellulase, pectinase, and alkaline protease is 3:3:5.

[0007] As a preferred technical solution of the present invention, the enzymolysis time of the cellulase is 3 h, the enzymolysis temperature is 40 °C, and the enzymolysis pH is 4.5.

[0008] As a preferred technical solution of the present invention, the enzymolysis time of the pectinase is 3 h, the enzymolysis temperature is 40 °C, and the enzymolysis pH is 4.0.

[0009] As a preferred technical solution of the present invention, the enzymolysis time of the alkaline protease is 2 h, the enzymolysis temperature is 45 °C, and the enzymolysis pH is 9.5.

[0010] As a preferred technical solution of the present invention, the agarwood essential oil includes (+)-3-carene, linalool, germacrene B, 2-phenylethyl-4H-benzopyran-4-one, 6-methoxy-2-(2-phenylethyl)chromone, 6,7-dimethoxy-2-(2-phenylethyl)chromone.

[0011] As a preferred technical solution of the present invention, the mass ratio of the (+)-3-carene, linalool, germacrene B, 2-phenylethyl-4H-benzopyran-4-one, 6-methoxy-2-(2-phenylethyl)chromone, 6,7-dimethoxy-2-(2-phenylethyl)chromone is 3.03:3.68:3.14:4.02:7.13:12.04.

[0012] The anti-aging agarwood composition prepared by the above preparation method.

[0013] The beneficial effects of the present invention: (1) An anti-aging agarwood composition and its preparation method disclosed by the present invention obtain agarwood essential oil by enzymatically hydrolyzing agarwood powder. Through the method of composite enzymatic hydrolysis, high contents of (+)-3-carene, linalool, germacrene B, 2-phenylethyl-4H-benzopyran-4-one, 6-methoxy-2-(2-phenylethyl)chromone, and 6,7-dimethoxy-2-(2-phenylethyl)chromone are obtained, which directly scavenge free radicals, activate the intracellular antioxidant enzyme system, enhance the body's own antioxidant capacity, reduce oxidative stress damage, stimulate fibroblasts to synthesize collagen, improve skin elasticity, reduce wrinkle formation, improve fine lines, and reduce inflammatory pigmentation.

[0014] (2) An anti-aging agarwood composition and its preparation method disclosed by the present invention further strengthen the antioxidant system, promote collagen synthesis, and inhibit melanin production through the synergistic effect among agarwood essential oil, Terminalia arjuna extract, pistachio seed oil, benzoin resin extract, Rhinacanthus nasutus extract, Spirulina platensis extract, Aloe vera flower extract, Abelmoschus manihot extract, Stachyurus himalaicus extract, and Eleutherococcus senticosus extract, and can better cope with the complex mechanism of skin aging compared with single components. Specific Embodiments

[0015] To further elaborate on the technical means and effects adopted by the present invention to achieve the predetermined invention purpose, the following examples are provided to illustrate in detail the specific embodiments, structures, features, and their effects according to the present invention. Examples

[0016] An anti-aging agarwood composition and its preparation method include the following steps: S1. Take 100 g of agarwood powder and disperse it in 500 mL of deionized water. Adjust the pH to 4.5 using a citric acid-sodium citrate buffer solution. Add 60,000 U of cellulase and enzymatically hydrolyze at 40°C for 3 h. Centrifuge and separate, collect the liquid phase, add one-quarter volume of anhydrous ether for static extraction, collect the upper layer liquid, repeat three times. Add an excessive amount of anhydrous sodium sulfate to the collected upper layer liquid to remove moisture. After standing and filtering, concentrate by rotary evaporation to obtain agarwood essential oil A; S2. Collect the solid phase after centrifugation and separation in S1, wash it with distilled water until the pH value is neutral, disperse it in 500 mL of deionized water, adjust the pH to 4.0 using a citric acid-sodium citrate buffer solution, add 60,000 U of pectinase, enzymatically hydrolyze at 40°C for 3 h. Centrifuge and separate, collect the liquid phase, add one-quarter volume of anhydrous ether for static extraction, collect the upper layer liquid, repeat three times. Add an excessive amount of anhydrous sodium sulfate to the collected upper layer liquid to remove moisture. After standing and filtering, concentrate by rotary evaporation to obtain agarwood essential oil B; S3. Collect the solid phase after centrifugation in S2 and wash it with distilled water until the pH value is neutral. Disperse it in 500 mL of deionized water, adjust the pH to 9.5 using a sodium phosphate - disodium hydrogen phosphate buffer solution, add 100,000 U of alkaline protease, enzymatically hydrolyze at 45 °C for 2 h, perform centrifugal separation, collect the liquid phase, add one - quarter volume of anhydrous ether for static extraction, collect the upper layer liquid, repeat three times, add excessive anhydrous sodium sulfate to the collected upper layer liquid to remove moisture, after standing and filtering, concentrate by rotary evaporation to obtain agarwood essential oil C; S4. Mix agarwood essential oil A, agarwood essential oil B, and agarwood essential oil C to obtain agarwood essential oil; the agarwood essential oil includes (+)-3 - carene, linalool, germacrene B, 2 - phenylethyl - 4H - benzopyran - 4 - one, 6 - methoxy - 2-(2 - phenylethyl)chromone, 6,7 - dimethoxy - 2-(2 - phenylethyl)chromone; the mass ratio of (+)-3 - carene, linalool, germacrene B, 2 - phenylethyl - 4H - benzopyran - 4 - one, 6 - methoxy - 2-(2 - phenylethyl)chromone, 6,7 - dimethoxy - 2-(2 - phenylethyl)chromone is 3.03:3.68:3.14:4.02:7.13:12.04.

[0017] S5. Take the agarwood essential oil, Terminalia arjuna extract, Pistacia vera seed oil, Styrax benzoin extract, Rhinacanthus nasutus extract, Spirulina platensis extract, Aloe vera flower extract, Abelmoschus manihot flower extract, Stachyurus himalaicus extract, Eleutherococcus senticosus extract, deionized water, anhydrous ethanol, and disodium laureth sulfosuccinate, mix them and stir for 20 min to obtain the anti - aging agarwood composition; The mass ratio of the agarwood essential oil, Terminalia arjuna extract, Pistacia vera seed oil, Styrax benzoin extract, Rhinacanthus nasutus extract, Spirulina platensis extract, Aloe vera flower extract, Abelmoschus manihot flower extract, Stachyurus himalaicus extract, Eleutherococcus senticosus extract, deionized water, anhydrous ethanol, and disodium laureth sulfosuccinate is 5:0.7:0.3:0.4:0.2:0.5:0.4:0.5:0.1:0.2:20:15:0.004. Example

[0018] The difference from Example 1 is that the mass ratio of the agarwood essential oil, Terminalia arjuna extract, Pistacia vera seed oil, Styrax benzoin extract, Rhinacanthus nasutus extract, Spirulina platensis extract, Aloe vera flower extract, Abelmoschus manihot flower extract, Stachyurus himalaicus extract, Eleutherococcus senticosus extract, deionized water, anhydrous ethanol, and disodium laureth sulfosuccinate is 7:0.8:0.4:0.5:0.25:0.6:0.5:0.7:0.2:0.3:25:20:0.005. Example

[0019] The difference from Example 1 is that the mass ratio of agarwood essential oil, Terminalia arjuna extract, pistachio seed oil, benzoin resin extract, Rhinacanthus nasutus extract, Spirulina platensis extract, Aloe vera flower extract, Calotropis procera flower extract, Stachyurus himalaicus extract, Eleutherococcus senticosus extract, deionized water, absolute ethanol, and disodium laureth sulfosuccinate is 10:0.9:0.5:0.6:0.3:0.7:0.6:0.9:0.3:0.5:30:25:0.006.

[0020] Comparative Example 1 The difference from Example 2 is that the mass ratio of agarwood essential oil, Terminalia arjuna extract, pistachio seed oil, benzoin resin extract, Rhinacanthus nasutus extract, Spirulina platensis extract, Aloe vera flower extract, Calotropis procera flower extract, Stachyurus himalaicus extract, Eleutherococcus senticosus extract, deionized water, absolute ethanol, and disodium laureth sulfosuccinate is 7:0:1.2:0.5:0.25:0.6:0.5:0.7:0.2:0.3:25:20:0.005.

[0021] Comparative Example 2 The difference from Example 2 is that the mass ratio of agarwood essential oil, Terminalia arjuna extract, pistachio seed oil, benzoin resin extract, Rhinacanthus nasutus extract, Spirulina platensis extract, Aloe vera flower extract, Calotropis procera flower extract, Stachyurus himalaicus extract, Eleutherococcus senticosus extract, deionized water, absolute ethanol, and disodium laureth sulfosuccinate is 7:0.8:0:0.9:0.25:0.6:0.5:0.7:0.2:0.3:25:20:0.005.

[0022] Comparative Example 3 The difference from Example 2 is that the mass ratio of agarwood essential oil, Terminalia arjuna extract, pistachio seed oil, benzoin resin extract, Rhinacanthus nasutus extract, Spirulina platensis extract, Aloe vera flower extract, Calotropis procera flower extract, Stachyurus himalaicus extract, Eleutherococcus senticosus extract, deionized water, absolute ethanol, and disodium laureth sulfosuccinate is 7:0.8:0.4:0:0.75:0.6:0.5:0.7:0.2:0.3:25:20:0.005.

[0023] Comparative Example 4 The difference from Example 2 lies in that the mass ratio of the agarwood essential oil, Terminalia arjuna extract, pistachio seed oil, benzoin resin extract, Rhinacanthus nasutus extract, Spirulina platensis extract, Aloe vera flower extract, Abelmoschus manihot flower extract, Stachyurus himalaicus extract, Eleutherococcus senticosus extract, deionized water, absolute ethanol, and disodium laureth sulfosuccinate is 7:0.8:0.4:0.5:0:0.85:0.5:0.7:0.2:0.3:25:20:0.005.

[0024] Comparative Example 5 The difference from Example 2 lies in that the mass ratio of the agarwood essential oil, Terminalia arjuna extract, pistachio seed oil, benzoin resin extract, Rhinacanthus nasutus extract, Spirulina platensis extract, Aloe vera flower extract, Abelmoschus manihot flower extract, Stachyurus himalaicus extract, Eleutherococcus senticosus extract, deionized water, absolute ethanol, and disodium laureth sulfosuccinate is 7:0.8:0.4:0.5:0.25:0:1.1:0.7:0.2:0.3:25:20:0.005.

[0025] Comparative Example 6 The difference from Example 2 lies in that the mass ratio of the agarwood essential oil, Terminalia arjuna extract, pistachio seed oil, benzoin resin extract, Rhinacanthus nasutus extract, Spirulina platensis extract, Aloe vera flower extract, Abelmoschus manihot flower extract, Stachyurus himalaicus extract, Eleutherococcus senticosus extract, deionized water, absolute ethanol, and disodium laureth sulfosuccinate is 7:0.8:0.4:0.5:0.25:0.6:0:1.2:0.2:0.3:25:20:0.005.

[0026] Comparative Example 7 The difference from Example 2 lies in that the mass ratio of the agarwood essential oil, Terminalia arjuna extract, pistachio seed oil, benzoin resin extract, Rhinacanthus nasutus extract, Spirulina platensis extract, Aloe vera flower extract, Abelmoschus manihot flower extract, Stachyurus himalaicus extract, Eleutherococcus senticosus extract, deionized water, absolute ethanol, and disodium laureth sulfosuccinate is 7:0.8:0.4:0.5:0.25:0.6:0.5:0:0.9:0.3:25:20:0.005.

[0027] Comparative Example 8 The difference from Example 2 lies in that the mass ratio of the agarwood essential oil, Terminalia arjuna extract, pistachio seed oil, benzoin resin extract, Rhinacanthus nasutus extract, Spirulina platensis extract, Aloe vera flower extract, Calotropis gigantea flower extract, Stachyurus himalaicus extract, Eleutherococcus senticosus extract, deionized water, absolute ethanol, and disodium laureth sulfosuccinate is 7:0.8:0.4:0.5:0.25:0.6:0.5:0.7:0:0.5:25:20:0.005.

[0028] Comparative Example 9 The difference from Example 2 lies in that the mass ratio of the agarwood essential oil, Terminalia arjuna extract, pistachio seed oil, benzoin resin extract, Rhinacanthus nasutus extract, Spirulina platensis extract, Aloe vera flower extract, Calotropis gigantea flower extract, Stachyurus himalaicus extract, Eleutherococcus senticosus extract, deionized water, absolute ethanol, and disodium laureth sulfosuccinate is 7:0.8:0.7:0.5:0.25:0.6:0.5:0.7:0.2:0:25:20:0.005.

[0029] Comparative Example 10 The difference from Example 2 lies in that the preparation method of the agarwood essential oil includes the following steps: S1. Take 100 g of agarwood wood powder and disperse it in 500 mL of deionized water. Adjust the pH to 4.5 using a citric acid - sodium citrate buffer solution. Add 220,000 U of cellulase and enzymolyze at 40 °C for 8 h. Centrifuge and separate to collect the liquid phase. Add one - quarter volume of absolute ether for static extraction, collect the upper layer liquid, repeat three times. Add an excessive amount of anhydrous sodium sulfate to the collected upper layer liquid to remove water. After standing and filtering, concentrate by rotary evaporation to obtain the agarwood essential oil.

[0030] Comparative Example 11 The difference from Example 2 lies in that the preparation method of the agarwood essential oil includes the following steps: Take 100 g of agarwood wood powder and disperse it in 500 mL of deionized water. Adjust the pH to 4.0 using a citric acid - sodium citrate buffer solution. Add 220,000 U of pectinase and enzymolyze at 40 °C for 8 h. Centrifuge and separate to collect the liquid phase. Add one - quarter volume of absolute ether for static extraction, collect the upper layer liquid, repeat three times. Add an excessive amount of anhydrous sodium sulfate to the collected upper layer liquid to remove water. After standing and filtering, concentrate by rotary evaporation to obtain the agarwood essential oil.

[0031] Comparative Example 12 The difference from Example 2 lies in that the preparation method of the agarwood essential oil includes the following steps: S1. Disperse 100 g of agarwood powder in 500 mL of deionized water, adjust the pH to 9.5 using a sodium phosphate - disodium hydrogen phosphate buffer solution, add 220,000 U of alkaline protease, enzymatically hydrolyze at 45 °C for 8 h, perform centrifugal separation, collect the liquid phase, add one - quarter volume of anhydrous ether for static extraction, collect the upper layer liquid, repeat three times, add an excessive amount of anhydrous sodium sulfate to the collected upper layer liquid to remove water, after standing and filtering, concentrate by rotary evaporation to obtain agarwood essential oil.

[0032] Comparative Example 13 The difference from Example 2 is that the preparation method of the agarwood essential oil includes the following steps: S1. Disperse 100 g of agarwood powder in 500 mL of deionized water, adjust the pH to 4.5 using a citric acid - sodium citrate buffer solution, add 110,000 U of cellulase, enzymatically hydrolyze at 40 °C for 4 h, perform centrifugal separation, collect the liquid phase, add one - quarter volume of anhydrous ether for static extraction, collect the upper layer liquid, repeat three times, add an excessive amount of anhydrous sodium sulfate to the collected upper layer liquid to remove water, after standing and filtering, concentrate by rotary evaporation to obtain agarwood essential oil A; S2. Collect the solid phase after centrifugal separation in S1 and wash it with distilled water until the pH value is neutral, disperse it in 500 mL of deionized water, adjust the pH to 4.0 using a citric acid - sodium citrate buffer solution, add 110,000 U of pectinase, enzymatically hydrolyze at 40 °C for 4 h, perform centrifugal separation, collect the liquid phase, add one - quarter volume of anhydrous ether for static extraction, collect the upper layer liquid, repeat three times, add an excessive amount of anhydrous sodium sulfate to the collected upper layer liquid to remove water, after standing and filtering, concentrate by rotary evaporation to obtain agarwood essential oil B; S3. Mix agarwood essential oil A and agarwood essential oil B to obtain agarwood essential oil.

[0033] Comparative Example 14 The difference from Example 2 is that the preparation method of the agarwood essential oil includes the following steps: S1. Disperse 100 g of agarwood powder in 500 mL of deionized water, adjust the pH to 4.0 using a citric acid - sodium citrate buffer solution, add 90,000 U of cellulase, enzymatically hydrolyze at 40 °C for 4.5 h, perform centrifugal separation, collect the liquid phase, add one - quarter volume of anhydrous ether for static extraction, collect the upper layer liquid, repeat three times, add an excessive amount of anhydrous sodium sulfate to the collected upper layer liquid to remove water, after standing and filtering, concentrate by rotary evaporation to obtain agarwood essential oil A; S2. Collect the solid phase after centrifugation in S1 and wash it with distilled water until the pH value is neutral. Disperse it in 500 mL of deionized water, adjust the pH to 9.5 using sodium phosphate - disodium hydrogen phosphate buffer, add 130,000 U of alkaline protease, enzymatically hydrolyze at 45 °C for 3.5 h, perform centrifugation separation, collect the liquid phase, add one - quarter volume of anhydrous ether for static extraction, collect the upper - layer liquid, repeat three times, add excessive anhydrous sodium sulfate to the collected upper - layer liquid to remove water, let it stand and filter, and then concentrate by rotary evaporation to obtain agarwood essential oil C; S4. Mix agarwood essential oil A and agarwood essential oil C to obtain agarwood essential oil.

[0034] Comparative Example 15 The difference from Example 2 is that the preparation method of the agarwood essential oil includes the following steps: S1. Take 100 g of agarwood wood powder and disperse it in 500 mL of deionized water, adjust the pH to 4.0 using citric acid - sodium citrate buffer, add 90,000 U of pectinase, enzymatically hydrolyze at 40 °C for 4.5 h, perform centrifugation separation, collect the liquid phase, add one - quarter volume of anhydrous ether for static extraction, collect the upper - layer liquid, repeat three times, add excessive anhydrous sodium sulfate to the collected upper - layer liquid to remove water, let it stand and filter, and then concentrate by rotary evaporation to obtain agarwood essential oil B; S2. Collect the solid phase after centrifugation in S1 and wash it with distilled water until the pH value is neutral. Disperse it in 500 mL of deionized water, adjust the pH to 9.5 using sodium phosphate - disodium hydrogen phosphate buffer, add 130,000 U of alkaline protease, enzymatically hydrolyze at 45 °C for 3.5 h, perform centrifugation separation, collect the liquid phase, add one - quarter volume of anhydrous ether for static extraction, collect the upper - layer liquid, repeat three times, add excessive anhydrous sodium sulfate to the collected upper - layer liquid to remove water, let it stand and filter, and then concentrate by rotary evaporation to obtain agarwood essential oil C; S4. Mix agarwood essential oil B and agarwood essential oil C to obtain agarwood essential oil.

[0035] Performance Test Test Example 1 DPPH Inhibition Rate Test Judge the ability of the sample to scavenge free radicals through DPPH. The higher the inhibition rate of DPPH, the better the antioxidant effect of the sample. The test results are shown in Table 1: Table 1

[0037] Test Example 2 Anti - Aging Detection Volunteers aged 35 - 55 years old were selected. The skin of the volunteers was healthy without damage, had wrinkles, and had similar skin textures. The volunteers were grouped, with 10 people in each group. After facial cleansing in the morning and evening for each group of volunteers, the fixed half-face comparison test method was adopted. The agarwood compositions prepared in the examples and comparative examples were respectively applied to the corresponding same side face, and the other side face was washed with clean water.

[0038] A skin elasticity tester was used to generate a negative pressure on the surface of the tested skin to suck the skin into a specific test probe. The depth at which the skin was sucked into the test probe was measured by a non-contact optical test system. The test probe included a light emitter and a receiver. The ratio of light (the ratio of emitted light to received light) was proportional to the depth of the skin sucked in. Then, through MPA software analysis, the elastic properties of the skin were determined. The skin elasticity index of the forehead area of the volunteers was measured before using the agarwood composition and 8 weeks after use. Each group was tested in parallel three times, and the test results were averaged. Then, the relative change in the skin elasticity index after 8 weeks was calculated; The skin chromaticity of the left and right cheeks was measured by a skin colorimeter, and in combination with the skin analysis software CM - SA, the difference in skin melanin content (MI value) was calculated; The test results are shown in Table 2 below: Table 2

[0039] As can be seen from Tables 1 - 3, the agarwood compositions prepared in Examples 1 - 3 of the present application have good effects of anti-aging, removing melanin, repairing wrinkles and tightening the skin; in Comparative Examples 1 - 9, the lack of the synergistic effect between agarwood essential oil and Terminalia arjuna extract, Pistacia vera seed oil, Styrax benzoin resin extract, Rhinacanthus nasutus extract, Spirulina platensis extract, Aloe vera flower extract, Abelmoschus manihot extract, Stachyurus himalaicus extract, and Eleutherococcus senticosus extract led to a decline in the anti-aging effect; in Comparative Examples 10 - 15, due to different degrees of enzymatic hydrolysis, the contents of key anti-aging components (+)-3-carene, linalool, germacrene B, 2-phenylethyl-4H-chromen-4-one, 6-methoxy-2-(2-phenylethyl)chromone, and 6,7-dimethoxy-2-(2-phenylethyl)chromone in agarwood essential oil decreased, resulting in a significant decline in the anti-aging effect.

[0040] The above are only the preferred embodiments of the present invention and do not impose any formal limitations on the present invention. Although the present invention has been disclosed above in its preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can make some changes or modifications to equivalent embodiments by using the disclosed technical content without departing from the technical solution of the present invention. However, as long as it does not depart from the technical solution content of the present invention, any brief modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention still fall within the scope of the technical solution of the present invention.

Claims

1. A preparation method of an anti-aging agarwood composition, characterized in that, It includes the following steps: Take agarwood powder and subject it to enzymatic hydrolysis with cellulase, pectinase, and alkaline protease in sequence, collect the product to obtain agarwood essential oil; Take the obtained agarwood essential oil, Terminalia arjuna extract, pistachio seed oil, benzoin resin extract, Rhinacanthus nasutus extract, Spirulina platensis extract, Aloe vera flower extract, Calotropis procera flower extract, Stachyurus himalaicus extract, Eleutherococcus senticosus extract, deionized water, absolute ethanol, and disodium laureth sulfosuccinate, mix them evenly, and then the anti-aging agarwood composition is obtained.

2. The preparation method of an anti-aging agarwood composition according to claim 1, wherein The mass ratio of the agarwood essential oil, Terminalia arjuna extract, pistachio seed oil, benzoin resin extract, Rhinacanthus nasutus extract, Spirulina platensis extract, Aloe vera flower extract, Calotropis procera flower extract, Stachyurus himalaicus extract, Eleutherococcus senticosus extract, deionized water, absolute ethanol, and disodium laureth sulfosuccinate is 5 - 10: 0.7 - 0.9: 0.3 - 0.5: 0.4 - 0.6: 0.2 - 0.3: 0.5 - 0.7: 0.4 - 0.6: 0.5 - 0.9: 0.1 - 0.3: 0.2 - 0.5: 20 - 30: 15 - 25: 0.004 - 0.

006.

3. The preparation method of an anti-aging agarwood composition according to claim 1, wherein, The dosage ratio of the cellulase, pectinase, and alkaline protease is 3: 3:

5.

4. The preparation method of an anti-aging agarwood composition according to claim 1, characterized in that, The enzymatic hydrolysis time of the cellulase is 3 h, the enzymatic hydrolysis temperature is 40 °C, and the enzymatic hydrolysis pH is 4.

5.

5. The preparation method of an anti-aging agarwood composition according to claim 1, wherein, The enzymatic hydrolysis time of the pectinase is 3 h, the enzymatic hydrolysis temperature is 40 °C, and the enzymatic hydrolysis pH is 4.

0.

6. The preparation method of an anti-aging agarwood composition according to claim 1, characterized in that, The enzymatic hydrolysis time of the alkaline protease is 2 h, the enzymatic hydrolysis temperature is 45 °C, and the enzymatic hydrolysis pH is 9.

5.

7. The preparation method of an anti-aging agarwood composition according to claim 1, characterized in that, The agarwood essential oil includes (+)-3-carene, linalool, germacrene B, 2-phenylethyl-4H-benzopyran-4-one, 6-methoxy-2-(2-phenylethyl)chromone, 6,7-dimethoxy-2-(2-phenylethyl)chromone.

8. The preparation method of an anti-aging agarwood composition according to claim 7, characterized in that, The mass ratio of the (+)-3-carene, linalool, germacrene B, 2-phenylethyl-4H-benzopyran-4-one, 6-methoxy-2-(2-phenylethyl)chromone, 6,7-dimethoxy-2-(2-phenylethyl)chromone is 3.03: 3.68: 3.14: 4.02: 7.13: 12.

04.

9. An anti-aging agarwood composition prepared by the preparation method according to any one of claims 1 - 8.

Images