Camellia composition nano-emulsion with anti-aging and repairing effects on fatigue skin as well as preparation and application of camellia composition nano-emulsion

Abstract

The invention belongs to the technical field of plant extraction and cosmetics, and particularly relates to camellia composition nano-emulsion with anti-aging and repairing effects on fatigue skin as well as a preparation method and application of the camellia composition nano-emulsion. The camellia flower extract, the camellia leaf extract, the camellia seed oil, the camellia seed extract and the PEG-40 hydrogenated castor oil are used as raw materials and are compounded according to a certain dosage to prepare the nano-emulsion, and the nano-emulsion has multiple effects of moisturizing, tightening, removing acnes and the like; the skin care product solves the problems that a conventional skin care product is not remarkable enough in fatigue muscle improvement effect, not quick enough in effect and insufficient in pertinence, and the skin is restored to be tender, moisturized and bright.

Description

Technical Field

[0001] The present invention belongs to the technical fields of plant extraction and cosmetics, and relates to a nanoemulsion of a camellia composition and its preparation and application; specifically, it relates to a nanoemulsion of a camellia composition with anti-aging and repair effects on tired skin (such as skin after staying up late), and its preparation method and application. Background Art

[0002] With the rapid development of society, the enrichment of lifestyle and the acceleration of the pace of life, staying up late and high life pressure have become the norm for young people, which has a great negative impact on the body's functions and skin conditions, especially causing problems such as dryness, roughness, and dullness of the skin, thus resulting in tired skin.

[0003] Research shows that the main physiological characteristics of tired skin are damaged skin barrier, reduced water content in the stratum corneum, increased transepidermal water loss, and increased sebum production. In addition, the firmness of people with tired skin is worse than that of people without tired skin. After young people's skin shows signs of tiredness, they will take different self-help measures, including diet therapy, exercise, adjusting work and rest, and using skin care products. In terms of the efficacy and usage scenarios of skin care products, targeted and fast and efficient first-aid skin care products are the best choice for the maintenance of young people's tired skin. However, with the improvement of living standards and the accumulation of human scientific knowledge, people's requirements for skin care products have changed greatly. Currently, commonly used skin care products have unsatisfactory repair effects on tired skin. How to design a product that is highly efficient, mild, natural, and safe for anti-aging and repairing tired skin is an inevitable trend in the development of tired skin care products. Summary of the Invention

[0004] The purpose of the present invention is to provide a nanoemulsion of a camellia composition with anti-aging and repair effects on tired skin, and its preparation method and application; the nanoemulsion has the characteristics of small particle size, high stability, and good water solubility, is safe, mild, and has little irritation, and at the same time has the effects of moisturizing, firming, and removing acne, integrating multiple effects, quickly and efficiently firming and repairing tired skin, and the effect is remarkable.

[0005] The purpose of the present invention is achieved by the following technical solutions:

[0006] The present invention provides a nanoemulsion of a camellia composition, and the raw materials according to mass parts include the following components: a total of 60-70 parts of camellia flower and camellia leaf extracts, 10-18 parts of camellia seed oil, 6-12 parts of camellia seed extract, and 13-17 parts of PEG-40 hydrogenated castor oil.

[0007] According to mass parts, the raw materials of the nanoemulsion of the camellia composition of the present invention include camellia flower and camellia leaf extracts as the aqueous phase, accounting for 60-70 parts, preferably 60-65 parts, and more preferably 65 parts.

[0008] Based on the mass parts of the aqueous phase, the raw material of the camellia composition nanoemulsion of the present invention uses camellia seed oil as the oil phase, accounting for 10 - 18 parts, preferably 12 - 15 parts of camellia seed oil, and more preferably 12 parts.

[0009] Based on the mass parts of the aqueous phase, in the raw material of the camellia composition nanoemulsion of the present invention, PEG - 40 hydrogenated castor oil can also be used as the surfactant, accounting for 13 - 17 parts, preferably 14 - 16 parts, and more preferably 15 parts.

[0010] Based on the mass parts of the aqueous phase, in the raw material of the camellia composition nanoemulsion of the present invention, camellia seed extract can also be used as the co - surfactant, accounting for 6 - 12 parts, preferably 8 - 10 parts, and more preferably 8 parts.

[0011] The present invention uses camellia flower extract, camellia leaf extract, camellia seed oil, camellia seed extract and PEG - 40 hydrogenated castor oil as raw materials, and prepares a nanoemulsion according to a certain dosage. It has multiple effects such as moisturizing, firming, and acne - removing, and can be used for the repair of skin damaged by staying up late, restoring the skin to be delicate, moist and bright.

[0012] The present invention also provides a preparation method of the aforementioned camellia composition nanoemulsion, including the following steps:

[0013] S1. Add PEG - 40 hydrogenated castor oil and camellia seed extract to the camellia flower and camellia leaf extracts, and stir and dissolve under heating conditions to obtain a mixture solution;

[0014] S2. Heat and stir the camellia seed oil, and slowly add the mixture solution drop by drop into the camellia seed oil, and stir until it changes from an opaque liquid to a transparent solution, then the camellia composition nanoemulsion is obtained.

[0015] As an embodiment, the heating temperature is 50 - 70 °C; more preferably, the heating temperature is 65 °C.

[0016] As an embodiment, the dropping speed is 60 - 120 mL / min, and it is added drop by drop at a constant speed.

[0017] As an embodiment, the camellia flower and camellia leaf extracts are prepared by a method including the following steps: Mix camellia flower, camellia leaf and water to obtain a premix, and carry out enzymatic hydrolysis on the premix; Carry out ultrasonic extraction on the enzymatic hydrolysis product, and take the supernatant after separating the extraction solution; This supernatant is the camellia flower extract and camellia leaf extract, which are jointly used as the aqueous phase of the nanoemulsion.

[0018] As an embodiment, the total mass ratio of camellia flower and camellia leaf to the volume of water is 1 g : (10 - 15) mL, and the ratio of camellia flower to camellia leaf is 2 : 3.

[0019] As an embodiment, the enzyme used in the enzymatic hydrolysis is a complex enzyme, and the complex enzyme includes bromelain and papain.

[0020] As an embodiment, the mass ratio of bromelain to papain in the complex enzyme is (2 - 4):(2 - 5); more preferably 3:4.

[0021] As an embodiment, the enzyme activity of bromelain in the complex enzyme is independently 2000 - 5000 u / g, more preferably 3000 u / g.

[0022] As an embodiment, the enzyme activity of papain in the complex enzyme is independently 50 - 1,000,000 u / g, more preferably 800,000 u / g.

[0023] As an embodiment, the mass ratio of the complex enzyme to the premix is 1.2 - 4.5:1000; more preferably 3.2:1000.

[0024] As an embodiment, the pH of the enzymatic hydrolysis is 3.5 - 5.5, more preferably 4.0 - 5.0, even more preferably 4.5; the time of the enzymatic hydrolysis is preferably 30 - 70 min, more preferably 40 - 60 min, even more preferably 43 - 50 min, most preferably 43 min; the temperature of the enzymatic hydrolysis is preferably 35 - 55 °C, more preferably 40 - 50 °C, even more preferably 45 °C.

[0025] As an embodiment, the power of the ultrasonic extraction is 400 w, and the temperature is 55 - 70 °C, more preferably 60 - 65 °C.

[0026] As an embodiment, the number of times of ultrasonic extraction is 2 times, and the time of each ultrasonic extraction is 20 - 30 min.

[0027] As an embodiment, the preferred separation method is centrifugation; the rotation speed of the centrifugation is preferably 6000 - 8500 rpm, more preferably 6500 - 8000 rpm, even more preferably 7000 - 7500 rpm; the time of the centrifugation is preferably 8 - 15 min, more preferably 10 - 12 min.

[0028] As an embodiment, the camellia seed oil and the camellia seed extract are prepared by a method comprising the following steps:

[0029] After the camellia seeds are shelled and crushed, additives are added and heated with stirring. When the particles agglomerate and are difficult to stir, the supernatant and the precipitate are obtained after separation; the obtained crude supernatant oil is deacidified by molecular distillation to obtain camellia seed oil;

[0030] The obtained precipitate is heated and extracted with water, and the supernatant is separated; ethanol is added to the obtained supernatant and allowed to stand at low temperature, and then the precipitate is separated to obtain the camellia seed extract.

[0031] As an embodiment, the auxiliary agent is at least one of Span 20, Span 40, and Span 60; more preferably, it is Span 40.

[0032] As an embodiment, the heating temperature for heating and stirring is 40-60°C, more preferably 50°C.

[0033] As an embodiment, the stirring time for heating and stirring is 30-60 min, more preferably 50 min.

[0034] As an embodiment, the process conditions for molecular distillation are: distillation temperature 160-180°C, scraping film rotation speed 150-200 rpm, vacuum degree 10-15 Pa; more preferably 170°C, 180 rpm, 12 Pa. The proportion of deionized water added is 1:6-1:10, more preferably 1:8.

[0035] As an embodiment, the extraction conditions for hot extraction are extraction time 90-150 min, extraction temperature 82-92°C, more preferably extraction time 120 min, extraction temperature 90°C.

[0036] As an embodiment, the proportion of ethanol added is 1:2-1:5 (g / mL), more preferably 1:4 (g / mL).

[0037] As an embodiment, the conditions for standing at low temperature are standing time 12-24 h, standing temperature -4-4°C; more preferably standing time 24 h, standing temperature 0-4°C.

[0038] As an embodiment, the separation method is preferably centrifugation; the rotation speed of centrifugation is preferably 3500-4500 rpm, more preferably 4000 rpm; the centrifugation time is preferably 10-30 min, more preferably 15-25 min.

[0039] The present invention also provides the application of the above camellia composition nanoemulsion in the preparation of cosmetics.

[0040] As an embodiment, the mass percentage content of the camellia composition nanoemulsion in the cosmetics is 0.5%-5.0%.

[0041] The cosmetic preferably includes essence, facial mask or lotion, and is further preferably essence. Using the above-mentioned nano-emulsion of camellia composition as the active ingredient of the cosmetic, the obtained cosmetic has various effects such as moisturizing, firming and acne removal, can specifically repair tired skin, has remarkable effects, quick results, is safe, mild and has little irritation, and can restore the delicate, moist and bright skin.

[0042] Compared with the prior art, the present invention has the following beneficial effects:

[0043] 1) The present invention uses camellia extract, camellia leaf extract, camellia seed oil, camellia seed extract and PEG-40 hydrogenated castor oil as raw materials, and is compounded in a certain amount to have various effects such as moisturizing, firming and acne removal, solving the problems that conventional skin care products have insufficiently significant improvement effect on tired skin, slow results and lack of pertinence, and restoring the delicate, moist and bright skin.

[0044] 2) The present invention uses a combination of multiple extraction processes to extract the raw materials, and the types of active ingredients are rich. At the same time, PEG-40 hydrogenated castor oil and camellia seed extract with a high polysaccharide content also play the roles of surfactant and co-surfactant respectively. The obtained oil-in-water nano-emulsion has a stable system, good percutaneous penetration effect, faster results, further improves the remarkable anti-aging and repair effect on tired skin, and at the same time, the extracted components are all of plant origin, safe, mild and have little irritation.

[0045] 3) The present invention actually detected the percutaneous penetration effect of the obtained nano-emulsion, confirmed that the system can significantly improve the subcutaneous absorption and diffusion of the active ingredient, and at the same time prepared the corresponding basic formula, clarifying that this system can be actually used in daily chemical products, and has the practical significance of being able to expand production and enter the market. Description of the Drawings

[0046] By reading the detailed description of the non-restrictive embodiments with reference to the following drawings, other features, purposes and advantages of the present invention will become more obvious:

[0047] Figure 1 It is the particle size detection diagram of the nano-emulsion in Example 1;

[0048] Figure 2 It is the percutaneous penetration ability of chlorophyll;

[0049] Figure 3 It is the percutaneous penetration ability of the nano-emulsion obtained in Example 1. Detailed Embodiments

[0050] The present invention will be described in detail below in conjunction with embodiments. The following embodiments will help those skilled in the art to further understand the present invention, but do not limit the present invention in any form. It should be noted that for those of ordinary skill in the art, without departing from the concept of the present invention, several adjustments and improvements can still be made. These all belong to the protection scope of the present invention.

[0051] Example 1

[0052] A preparation method of a nanoemulsion of a camellia composition with anti-aging and repair effects on tired skin comprises the following steps:

[0053] 1. Remove impurities, dry, and pulverize camellia flowers and camellia leaves respectively. The pulverization degree should be such that both can pass through a 40-mesh sieve. Mix the powders evenly to obtain a pre-mix of camellia flowers and leaves; the mass ratio of camellia flower and leaf powders in the pre-mix is 2:3.

[0054] 2. Mix the pre-mix and deionized water according to a mass-volume ratio of 1 g:13 mL to obtain a pre-mixture, and adjust the pH to 5.5; mix the pre-mixture and the complex enzyme according to a mass ratio of 3.2 mg:1 g, and enzymatically hydrolyze at 50 °C for 43 min to obtain an enzymatically hydrolyzed product; the complex enzyme is composed of bromelain and papain, and the mass ratio is 3:4; the enzyme activity of bromelain is independently 3000 u / g; the enzyme activity of papain is independently 800,000 u / g; under the condition of a power of 400 w, ultrasonically extract the enzymatically hydrolyzed product 2 times, combine the two extraction liquids, centrifuge at 8000 rpm for 15 min, and collect the supernatant and precipitate; among them, the time for each ultrasonic extraction is 25 min, and during each ultrasonic extraction process, the temperature is uniformly increased from 45 °C to 70 °C at a heating rate of 5 °C / 10 min. The obtained supernatant is the extract of camellia flowers and leaves.

[0055] 3. After shelling camellia seeds, add span 40 according to a solid-liquid ratio of 5:1, stir at 50 °C for 50 minutes, then centrifuge at 4000 rpm for 20 min. The obtained supernatant is subjected to molecular distillation at a distillation temperature of 170 °C, a scraping film speed of 180 rpm, and a vacuum degree of 12 Pa to obtain camellia seed oil; the obtained precipitate is added with deionized water according to a ratio of 1:8, extracted at 90 °C for 120 minutes, centrifuged at 4000 rpm for 20 min, and then ethanol is added to the supernatant according to a solid-liquid ratio of 1:4 (g / mL), and allowed to stand at 0-4 °C for 24 hours. Subsequently, centrifuge at 4000 rpm for 20 min to obtain the precipitate, which is the extract of camellia seeds.

[0056] 4. Prepare materials according to the following parts by mass: a total of 65 parts of camellia extract and camellia leaf extract, 12 parts of camellia seed oil, 8 parts of camellia seed extract, and 15 parts of PEG-40 hydrogenated castor oil. Add PEG-40 hydrogenated castor oil and camellia seed extract to the camellia extract and camellia leaf extract in proportion, and fully stir and dissolve at 65 °C to obtain a mixture solution. Heat and stir the camellia seed oil, and slowly add the mixture solution drop by drop to the camellia seed oil. Finally, stir until the appearance changes from an opaque liquid to a transparent solution. The obtained transparent solution is detected by a Malvern particle size analyzer, and the particle size of the obtained transparent solution is less than 300 nm (see Figure 1 ), and then the nanoemulsion of the camellia composition is obtained.

[0057] Example 2

[0058] Same as Example 1, the only difference is that the materials are prepared according to the following parts by mass:

[0059] 70 parts of camellia extract and camellia leaf extract, 10 parts of camellia seed oil, 6 parts of camellia seed extract, and 14 parts of PEG-40 hydrogenated castor oil. The preparation method is the same.

[0060] Example 3

[0061] Same as Example 1, the only difference is that the materials are prepared according to the following parts by mass:

[0062] 60 parts of camellia extract and camellia leaf extract, 18 parts of camellia seed oil, 7 parts of camellia seed extract, and 15 parts of PEG-40 hydrogenated castor oil. The preparation method is the same.

[0063] Test Example 1

[0064] Moisturizing effect detection

[0065] 1. Principle

[0066] Based on the measurement of the water content of the skin stratum corneum by a skin moisture tester, the moisturizing effect is detected.

[0067] 2. Instruments and materials

[0068] Skin moisture tester, analytical balance (accuracy 0.1 mg).

[0069] 1. Test steps

[0070] 3.1. Subject conditions

[0071] 3.1.1 Inclusion criteria

[0072] The selected subjects should meet the following conditions:

[0073] 1) Aged 18 to 45 years old;

[0074] 2) Having a long history of staying up late, resulting in the skin being in a state of fatigue for a long time: going to bed after 0:00 every day within the past month, with a sleep duration of less than 5 hours, and maintaining the same state for the next 5 days.

[0075] 3.1.2 Preparation before testing

[0076] 1) The nano-emulsions of the camellia compositions obtained in Examples 1, 2, and 3 are used as test samples. The number of subjects for each test sample is not less than 30 valid cases. They are grouped according to the random table. When the subjects first visit, they are given an explanation of the test. After the subjects are fully informed, they sign a written informed consent form. A series of questions about the medical history, health status, etc. are asked of the subjects according to the inclusion and exclusion criteria, etc. At the same time, the skin of the test area is evaluated for compliance and screened and recorded.

[0077] 2) The test area on the subjects' faces should be cleaned with a unified facial cleansing sample before the test; sit quietly for 10 minutes in an environment with a constant temperature and humidity of (21±1)°C and a relative humidity of (50±5)%. Hot drinks or hot foods cannot be consumed during the test, and the test area should not get wet or be touched.

[0078] 3) After completing the quiet sitting, use a skin moisture tester to detect and record the initial value of the water content of the stratum corneum in the application area; select both cheeks of the subjects as the sample application area. First, detect and record the average value of the water content of the stratum corneum before the test, and then evenly apply 2 mL of the test sample to the sample application area once in the morning and once in the evening every day for 28 consecutive days. The subjects are informed in detail about the usage method and precautions, and the subjects use the sample under the guidance of the researcher. The water content of the stratum corneum of the subjects is detected and recorded during the follow-up visits on the 1st, 7th, 14th, and 28th days after use, and the average value is taken. The results are shown in Table 1.

[0079] Table 1 Average value of the water content of the skin stratum corneum (%)

[0080] sample before the test Day 1 Day 7 Day 14 Day 28 Example 1 41.37 57.58 59.33 60.55 62.1 Example 2 42.99 47.55 52.17 53.35 55.35 Example 3 43.13 49.56 52.58 56.44 59.21

[0081] As can be seen from Table 1, compared with the skin water content of the blank control group before the experiment, after using the nanoemulsion of the camellia composition obtained in Example 1, the water content of the stratum corneum of the facial skin shows a trend of first increasing, then decreasing and then increasing, indicating that the instant water replenishing effect of this sample is good. Because an important function of human skin is to lock water and keep it moist, when the epidermal keratinocytes are fully hydrated after using the test sample, the water content on the first day after using the test sample shows an increasing trend compared with before the experiment. The normal skin barrier function will prevent the water in the dermis from passing through the outermost keratinocytes. When the skin barrier function is not complete enough, the skin moisture will be lost, causing the skin to be in a water-deficient state. Compared with the blank control group, the experimental group shows an increasing trend on the 7th, 14th and 28th days, and with the increase of the use time, the skin water content gradually increases, indicating that during the process of using the sample, the skin barrier is gradually repaired, and the essence provided by the present invention has strong water-locking and moisturizing ability.

[0082] Test Example 2

[0083] Detection of firming effect

[0084] 1. Principle

[0085] Based on the principle of suction and stretching, a constant negative pressure is applied to the skin in the vertical direction, and then the negative pressure is removed. The displacement change of the skin within a specified time period is measured to verify the firming effect, and the skin elasticity characterization value R is obtained. The larger the skin elasticity characterization value R, the higher the skin elasticity. The calculation formula of the skin elasticity characterization value R: R = Ua / Uf, where Ua is the rebound displacement of the skin after removing the negative pressure, in millimeters (mm); Uf is the maximum stretching displacement of the skin when applying the negative pressure, in millimeters (mm). Calculate the difference between the initial value and the measured value at the set measurement time points on the sample application side and the control side respectively, and then use this difference to statistically analyze the difference between the sample application side and the control side at different measurement time points.

[0086] 2. Instruments and materials

[0087] 2.1 Instruments

[0088] Skin elasticity tester (ELASTIMETER ELM1086).

[0089] 2.2 Materials

[0090] Distilled water, lint-free absorbent dry paper towels, dust-free paper.

[0091] 2.3 Test steps

[0092] 2.3.1 Preparation before testing

[0093] The nanoemulsions of the camellia compositions obtained in Examples 1, 2, and 3 were used as test samples. Subjects were recruited according to the criteria of Test Example 1. For each sample, the number of valid subjects was not less than 30. They were grouped according to the random table. Before each test, the subjects uniformly cleaned their faces and blotted them dry with lint-free absorbent paper towels. They sat still in a standard test environment for at least 20 minutes, without drinking water or beverages. The subjects remained relaxed with their faces exposed and avoided touching. The left and right faces were randomly divided into the sample application side and the control side to ensure statistical balance. Measurement areas were determined on the left and right zygomatic regions, and the area of each measurement area was 3 cm × 3 cm.

[0094] 2.3.2 Skin elasticity parameter test

[0095] Each time, 2 mL of the sample was applied, once in the morning and once in the evening every day. Measurements were taken every other day. A skin elasticity tester was used to measure the skin elasticity value and skin firmness value of each test area. Three measurements were taken at different positions within the measurement area, and the average value was recorded. The entire test period was 14 days, and the results are shown in Tables 2 - 3 below.

[0096]

[0097]

[0098] It can be seen from Tables 2 - 3 that after using the test samples, the facial skin elasticity value and firmness value showed an upward trend, higher than the facial skin elasticity and firmness before using the samples. This indicates that after starting to use the test samples, to a certain extent, it promoted the production of collagen in the skin, endowing the skin tissue with elasticity and firmness.

[0099] Test Example 3

[0100] Human acne treatment efficacy test

[0101] 1) Subjects (special requirements)

[0102] Subjects were required to have only comedones (mild) or comedones accompanied by inflammatory papulopustules (moderate), specifically referring to the "Chinese Acne Treatment Guidelines (2019 Revised Edition)". The number of comedones in the entire evaluation area of the subjects was not less than 10 and not more than 20; they had not received physical / chemical treatments such as photon, blue light, chemical peeling, etc. within 1 month, and had not taken oral or topical facial corticosteroids, antibiotics, anti-inflammatory drugs, and retinoids such as tretinoin; they were able to understand the test process, voluntarily participated in the test, and signed a written informed consent form.

[0103] 2) Test design

[0104] The nano-emulsions of the camellia compositions obtained in Examples 1, 2, and 3 were used as test samples. The number of valid cases for each sample was not less than 30 subjects, and they were grouped according to a random table. When the acne areas on the left and right sides of the test site were basically symmetrical, 2 mL of the sample was applied each time, once in the morning and once in the evening every day. The subjects continuously used the test product for 4 weeks. The number of comedones was counted every week, and the results are shown in Table 4.

[0105] Table 4 Acne-removing effect of the nano-emulsion of the camellia composition (pcs)

[0106] Example 1 Example 2 Example 3 before the test 16 18 17 Day 7 14 16 16 Day 14 12 14 13 Day 21 9 11 10 Day 28 5 10 9

[0107] As can be seen from Table 4, the sample of Example 1 showed a good acne-removing effect. The number of comedones in the test group continuously decreased over time, indicating a good acne-removing efficacy.

[0108] Example 4

[0109] A serum is composed of the following components: acrylate / C10-30 alkyl acrylate cross-linked polymer 0.3 wt.%, butylene glycol 4 wt.%, glycerol 2 wt.%, disodium EDTA 0.05 wt.%, phenoxyethanol / ethylhexylglycerin 0.6 wt.%, essence 0.1 wt.%, the nano-emulsion of the camellia composition obtained in Example 1 6 wt.%, and the balance deionized water.

[0110] The above serum is prepared by the following steps:

[0111] Sprinkle the acrylate / C10-30 alkyl acrylate cross-linked polymer evenly on the deionized water. After it is completely wetted, stir evenly. Swell at 80 °C until it is homogeneous and transparent, then add disodium EDTA and the nano-emulsion of the camellia composition obtained in Example 1, and stir evenly. Cool down to below 45 °C, add phenoxyethanol / ethylhexylglycerin, essence, and butylene glycol (the essence is pre-dissolved with butylene glycol), and stir evenly to obtain the serum, denoted as Sample No. 1.

[0112] Comparative Example 1

[0113] Same as Example 4, the only difference is that the nano-emulsion of the plant composition camellia composition obtained in Example 1 is not used, denoted as Sample No. 2.

[0114] Test Example 4

[0115] 1. Requirements for human test subjects

[0116] Number of subjects: The number of valid subjects is not less than 30.

[0117] 2. Subject conditions

[0118] 2.1 Inclusion criteria

[0119] The selected subjects should meet the following conditions:

[0120] 1) Aged 18 - 45 years old;

[0121] 2) Having a long history of staying up late, resulting in the skin being in a fatigued state for a long time: going to bed after 0:00 every day within the past month, with a sleep duration of less than 5 hours, and maintaining the same state in the next 5 days.

[0122] 2.2 Preparation of Subjects before the Test

[0123] When the subject visits for the first time, the test instructions are given to the subject. After the subject is fully informed, a written informed consent form is signed. A series of questions about the medical history, health status, etc. are asked of the subject according to the inclusion and exclusion criteria, etc. At the same time, a compliance assessment and test screening of the skin at the test site are carried out and recorded.

[0124] Within 30 minutes before each round of testing, no drinking or eating is allowed. The area to be tested should be placed in an exposed state and kept relaxed.

[0125] 3. Combining the above requirements, a total of no less than 30 valid cases of volunteers are selected for this test. The skin conditions on the left and right sides of the face are similar. A comprehensive evaluation is carried out on the moisturizing, firming, and acne - removing effects of Example 4 and Comparative Example 5;

[0126] The volunteers apply the samples on the left and right sides. The 1st sample obtained from Example 4 is applied on the left side, and the 2nd sample obtained from Comparative Example 1 is applied on the right side. 2 mL of each sample is applied each time, and it is applied twice a day. All volunteers record the skin condition 2 hours after using the samples and record continuously for 28 days. Finally, a sample overall evaluation form is submitted for the comprehensive use effect in 28 days. The scoring criteria are shown in Table 5, and the scoring results are shown in Table 6.

[0127] Table 5 Evaluation Index

[0128]

[0129] Table 6 Overall Scoring Table for Using Samples 1 - 2

[0130]

[0131]

[0132] It can be seen from Table 6 that the 1st sample has a good effect of firming and repairing the fatigued skin, and has good effects in moisturizing, firming, and acne - removing; while the 2nd sample does not have a quick and effective firming and repairing effect on the fatigued skin. Thus, it can be seen that the essence provided by the present invention meets the product development requirements of being targeted, fast, and efficient in firming and repairing the fatigued skin.

[0133] As can be seen from the above, the technical solution provided by the present invention has multiple effects such as moisturizing, firming, brightening, and acne removal. It can specifically firm and repair tired skin, with remarkable effects, quick results, being safe, mild, and having little irritation, and can restore the delicate, moist, and bright skin.

[0134] Although the above embodiments have described the present invention in detail, they are only a part of the embodiments of the present invention, rather than all embodiments. People can also obtain other embodiments based on these embodiments without creative efforts, and these embodiments all fall within the protection scope of the present invention.

[0135] Test Example 5: Percutaneous Penetration Effect Experiment

[0136] C57BL / 6 mice (6 - 8 weeks old, male) were placed in a standard experimental animal room for adaptive feeding for 5 days. After observing that the mice had no adverse reactions, they were sacrificed by cervical dislocation. Subsequently, the hair on the back of the rats was carefully shaved off with a blade immediately (ensuring that the skin was intact without damage) to prepare rat skin with an inner diameter of 2.5 cm. Then, the skin in the shaved area of the rats was peeled off with experimental instruments, and the residual adipose tissue behind the skin was carefully removed repeatedly with forceps to ensure that the skin structure was uniform and complete. Subsequently, the surface of the removed skin was rinsed with physiological saline, and the excess moisture on the skin was blotted dry with filter paper and fixed on a TK12 - D diffusion cell with the stratum corneum facing the supply pool, and the subcutaneous receiving liquid was phosphate buffer solution (i.e., PBS). The nanoemulsion of Example 1 encapsulating chlorophyll and chlorophyll were respectively evenly applied on the surface of the rat skin in the diffusion cell. The water bath temperature was adjusted to 37 °C to maintain the skin surface temperature at (32 ± 1) °C. After 24 h, the rat skin was removed, and the residual dye on the surface was washed with PBS. The skin in the middle area was taken and preserved in paraformaldehyde. After the skin was ultrathin sectioned, it was placed on a glass slide and imaged using a confocal laser scanning microscope with an excitation wavelength of 460 nm.

[0137] The results showed that compared with the skin only smeared with chlorophyll ( Figure 2 ), after 24 h of smearing the chlorophyll solution encapsulated by the nanoemulsion of Example 1, fluorescence appeared in the dermis layer as seen from the skin tissue ( Figure 3 ). The results indicated that the nanoemulsion of Example 1 showed the property of promoting transdermal absorption.

[0138] The specific embodiments of the present invention have been described above. It should be understood that the present invention is not limited to the above - mentioned specific embodiments, and those skilled in the art can make various deformations or modifications within the scope of the claims, which do not affect the essence of the present invention.

Claims

1. A nanoemulsion of a camellia composition, characterized in that, Raw materials comprising the following components by mass parts: a total of 60 - 70 parts of camellia flower and camellia leaf extract, 10 - 18 parts of camellia seed oil, 6 - 12 parts of camellia seed extract, and 13 - 17 parts of PEG - 40 hydrogenated castor oil.

2. A method for preparing a nanoemulsion of the camellia composition according to claim 1, characterized in that, The method comprises the following steps: S1. Add PEG - 40 hydrogenated castor oil and camellia seed extract into the camellia flower and camellia leaf extract, and stir and dissolve under heating conditions to obtain a mixture solution; S2. Heat and stir the camellia seed oil, and slowly add the mixture solution drop by drop into the camellia seed oil, and stir until it changes from an opaque liquid to a transparent solution, thus obtaining the nanoemulsion of the camellia composition.

3. The preparation method of the nanoemulsion of the camellia composition according to claim 2, characterized in that, The heating temperature is 50 - 70°C; the dropping speed is 60 - 120 mL / min, and it is added drop by drop at a constant speed.

4. The preparation method of the nanoemulsion of the camellia composition according to claim 2, characterized in that, The camellia flower and camellia leaf extract is prepared by a method comprising the following steps: Mix camellia flower, camellia leaf and water to obtain a premix, and carry out enzymatic hydrolysis on the premix; carry out ultrasonic extraction on the enzymatic hydrolysis product, and take the supernatant after separation of the extract, thus obtaining it.

5. The preparation method of the nanoemulsion of the camellia composition according to claim 4, characterized in that, At least including one of the following technical features: A. The total mass ratio of camellia flower and camellia leaf to the volume of water is 1 g:(10 - 15) mL, and the ratio of camellia flower to camellia leaf is 2:3; B. The enzyme used for enzymatic hydrolysis is a composite enzyme, and the composite enzyme includes bromelain and papain; C. The enzyme used for enzymatic hydrolysis is a composite enzyme composed of bromelain and papain with a mass ratio of (2 - 4):(2 - 5); the enzyme activity of bromelain is independently 2000 - 5000 u / g; the enzyme activity of papain is independently 50 - 1 million u / g; D. The mass ratio of the composite enzyme used for enzymatic hydrolysis to the premix is 1.2 - 4.5:1000; E. The pH of the enzymatic hydrolysis is 3.5 - 5.5, the time is 30 - 70 min, and the temperature is 35 - 55°C; F. The power of the ultrasonic extraction is 400 w, and the temperature is 55 - 70°C; G. The number of times of ultrasonic extraction is 2 times, and the time of each ultrasonic extraction is 20 - 30 min.

6. The preparation method of the nanoemulsion of the camellia composition according to claim 2, characterized in that, The camellia seed oil and camellia seed extract are prepared by a method comprising the following steps: After shelling and crushing camellia seeds, add an auxiliary agent and heat and stir. When it is stirred until the particles aggregate into a mass and are difficult to stir, separate to obtain a supernatant and a precipitate; collect the obtained supernatant crude oil and carry out molecular distillation for deacidification to obtain camellia seed oil; add water to the obtained precipitate for hot extraction, and separate to take the supernatant; add ethanol to the obtained supernatant and let it stand at low temperature, then separate to take the precipitate, thus obtaining camellia seed extract.

7. The preparation method of the nanoemulsion of the camellia composition according to claim 6, characterized in that, At least including one of the following technical features: A. The auxiliary agent is at least one of span 20, span 40, and span 60; B. The heating temperature of the heating and stirring is 40 - 60°C, and the stirring time is 30 - 60 min; C. The process conditions of the molecular distillation are: distillation temperature 160 - 180°C, scraping film rotation speed 150 - 200 rpm, and vacuum degree 10 - 15 pa; D. The obtained precipitate is added with deionized water at a ratio of 1:8; the extraction time of the hot extraction is 90 - 150 min, and the extraction temperature is 82 - 92°C; E. Add ethanol to the obtained supernatant in a ratio of 1:2 to 1:5 (g / mL); F. The conditions for the low-temperature static placement are a static placement time of 12 to 24 h and a static placement temperature of -4 to 4 °C.

8. The preparation method of the nanoemulsion of the camellia composition according to claim 6, characterized in that, The separation method is centrifugation; the rotation speed of the centrifugation is 3500 to 4500 rpm, and the centrifugation time is 10 to 30 min.

9. Use of a nanoemulsion of a camellia composition as described in claim 1 or a nanoemulsion of a camellia composition prepared by the method described in any one of claims 2 to 78 in the preparation of cosmetics.

10. The application according to claim 9, characterized in that, The mass percentage content of the nanoemulsion of the camellia composition in the cosmetics is 0.5% to 5.0%.

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