3-ketosteroid-delta1-dehydrogenase mutant and application thereof in catalyzing dehydrogenation of steroid compound
Through directional evolution and optimization of the catalytic reaction system, an efficient 3-sterone-Δ1-dehydrogenase mutant was obtained, which solved the problem of low catalytic efficiency caused by substrate insolubleness and achieved a high conversion rate of C1 and 2-position dehydrogenation reaction.
Patent Information
- Application Number
- CN202510513848.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-04-23
- Publication Date
- 2025-07-25
- Estimated Expiration
- 2045-04-23
AI Technical Summary
The prior art is difficult to efficiently catalyze the dehydrogenation reaction of (16α)-methylandrost-4,9-diene-3,17-dione at the C1,2 position, and the substrate is difficult to dissolve in water, resulting in low biocatalytic efficiency.
PrKstD, a mutant 3-sterone-Δ1-dehydrogenase, was obtained through directed evolution, and a recombinant expression vector was constructed and the catalytic reaction system was optimized. The cosolvent Tween-80 was added to improve substrate solubility, and the induction conditions were optimized to improve catalytic efficiency.
The conversion rate of (16α)-methylandrost-4,9-diene-3,17-dione was achieved to reach 99.9%, solving the problem of substrate insolubleness, and providing a green, environmentally friendly, safe and efficient biocatalytic path.