Probe and application thereof
By developing lectin-linker-metal chelate probes, combined with mass spectrometry flow and imaging technology, the high-throughput problem of single-cell sugar chain analysis is solved, and efficient identification of multiple sugar chains and detection of sugar chain distribution at the biological tissue level is achieved.
Patent Information
- Application Number
- CN202510466212.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Priority Date
- 2024-04-12
- Filing Date
- 2025-04-14
- Publication Date
- 2025-07-25
AI Technical Summary
The prior art has few reports on high-throughput sugar chain analysis technology at the single-cell level, and the lectin index based on mass spectrometry flow technology is limited. The identified sugar chains overlap, making it impossible to effectively perform high-throughput sugar chain analysis.
A probe was developed with a structure of lectin-linker-metal chelate, which was selected from a variety of types, and metal chelate was a specific element, and high-throughput sugar chain analysis was performed through mass spectrometry flow technology and mass spectrometry imaging technology.
High-throughput, systematic single-cell glycomic identification and sugar chain distribution detection at the biological tissue level are achieved, which can identify multiple sugar chains, reduce interference, and improve detection accuracy and throughput.