Insecticidal compound composition containing propyl dihydrojasmonate and application thereof
Through the complex composition of propyl dihydrojasmonate and methylammonium benzoate, acetaminamid and cyanida bromide, the plant defense network is activated, and the problem of difficulty in preventing and controlling thrips and chemical pesticide resistance is solved, and efficient and environmentally friendly insecticidal effects and plant growth promotion are achieved.
Patent Information
- Application Number
- CN202510532247.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-04-24
- Publication Date
- 2025-08-01
AI Technical Summary
In the existing technology, the difficulty of preventing and controlling thrips is increased, the resistance of chemical pesticides is serious, the cost of biological and physical control is high, the timeliness is poor, and the lack of efficient and environmentally friendly prevention and control technology.
The complex composition of propyl dihydrojasmonate, methylammonicin benzoate, acetaminamidine, and cyanidaminamide is used to activate the plant defense network by simulating jasmonic acid signals, enhance plant insect resistance, and perform multiple action mechanisms with tobacco thrips.
It significantly improves the prevention and control effect of tobacco thrips, reduces pesticide use, reduces pesticide residues, protects the ecological environment, and promotes plant growth and enhances stress resistance.
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Abstract
Description
Technical Field
[0001] The present invention belongs to the technical field of agriculture, and particularly relates to a synergistic compound composition containing propyl dihydrojasmonate insecticide and its application. Background Art
[0002] Thrips tabaci Western flower thrips ), as a global agricultural pest, is widely distributed in temperate and subtropical regions, posing a serious threat to economic crops such as Solanaceae (such as tomatoes, peppers), Cucurbitaceae (such as cucumbers, watermelons), Liliaceae (such as onions, garlic), as well as cotton and tobacco. Its damage methods mainly include directly feeding on plant leaves, flowers and fruits, and indirectly causing crop yield reduction or even crop failure by spreading plant viruses (such as Tomato spotted wilt virus TSWV). The global agricultural economic losses caused by Thrips tabaci exceed $2 billion annually. In pest-prone areas, the crop yield loss can reach 30%-50%. In recent years, with the aggravation of climate change, the expansion of protected agriculture and the problem of pesticide resistance, the control difficulty of Thrips tabaci has been significantly improved, and there is an urgent need to develop new, efficient and environmentally friendly prevention and control technologies.
[0003] At present, chemical pesticides are still the main means for controlling Thrips tabaci, but the long-term reliance on insecticides with a single mode of action (such as neonicotinoids, pyrethroids) has led to the widespread development of resistance in Thrips tabaci populations. Biological control (such as releasing Neoseiulus barkeri, Neoseiulus barkeri ), and physical control (such as blue sticky traps) have certain effects, but they often face problems such as high cost and poor timeliness in field applications.
[0004] Propyl dihydrojasmonate (PDJ) is a jasmonic acid derivative developed by Zeon Corporation of Japan, mainly used for coloring grapes, apples, winter jujubes, tomatoes, etc. Its chemical structural formula is shown below.
[0005]
[0006] Emamectin benzoate is a highly effective, low-toxic and broad-spectrum semi-synthetic antibiotic insecticide, which is synthesized by chemically modifying avermectin B1 and has a dual mode of action of stomach toxicity and contact toxicity. It activates the γ-aminobutyric acid receptor of pests, promotes a large amount of chloride ions to enter nerve cells, blocks the transmission of motor nerve signals, and causes pests to become paralyzed, stop feeding and die. Its application scope covers crops such as vegetables, fruit trees, cotton, rice and corn, mainly controlling Lepidoptera pests (such as Helicoverpa armigera, Spodoptera exigua, Plutella xylostella, Cnaphalocrocis medinalis), Coleoptera pests (such as Ostrinia furnacalis, Scarabaeidae), Diptera pests (such as Liriomyza sativae), as well as thrips and mites, etc. Its chemical structural formula is shown below.
[0007]
[0008] Acetamiprid is a new type of pyridine insecticide, belonging to nitro-methylene heterocyclic compounds. It acts on the nicotinic acetylcholine receptor at the synaptic site of the insect nervous system, interfering with the stimulation conduction of the insect nervous system, causing obstruction of the nervous system pathway, resulting in the accumulation of the neurotransmitter acetylcholine at the synaptic site, thereby causing insect paralysis and ultimately death. It has contact and stomach toxicity effects, and at the same time has a strong penetration effect, with good quick-acting property and long-lasting effect. Acetamiprid can be used to control aphids, planthoppers, thrips and Lepidoptera pests on rice, vegetables, fruit trees and tea trees. Its chemical structural formula is shown as follows.
[0009]
[0010] Cyantraniliprole is a second-generation ryanodine receptor inhibitor insecticide developed by DuPont Company of the United States. It belongs to benzamide compounds and has both contact, stomach toxicity and strong systemic activity. Its mechanism of action is to activate the ryanodine receptor in pests, triggering the irreversible release of calcium ions in striated muscle and smooth muscle cells, resulting in continuous contraction, paralysis and death of pests. Cyantraniliprole can control piercing-sucking mouthpart pests such as Lepidoptera (Helicoverpa armigera, Spodoptera exigua, Cnaphalocrocis medinalis), Hemiptera (aphids, whiteflies, Bemisia tabaci), Diptera (Liriomyza sativae, Drosophila melanogaster), Coleoptera (white grubs, beetles) and thrips, and is suitable for crops such as vegetables, fruit trees, cotton, rice, corn, etc. Its chemical structural formula is shown as follows.
[0011]
[0012] Chemical control is prone to the risk of drug resistance during the process of disease control, and the compounding of pesticide ingredients is an important means to delay the generation of drug resistance. Compounding different pesticide ingredients may produce some unexpected technical effects, which is also one of the effective methods for controlling Thrips tabaci. However, in the prior art, the compounding effects of the vast majority of pesticides are limited to additive effects, and there are few cases of pesticide compounding with significant synergistic effects. Moreover, so far, there is no report on the compound composition of propyl dihydrojasmonate with acetamiprid, emamectin benzoate and cyantraniliprole.
[0013] The information disclosed in this background art section is only intended to enhance the understanding of the overall background of the present invention and should not be regarded as an admission or any form of implication that this information constitutes the prior art already known to those of ordinary skill in the art. Summary of the Invention
[0014] The present invention aims to address the deficiencies of the existing technology by providing a synergistic compound composition containing propyl dihydrojasmonate insecticide and its application. The present invention provides a propyl dihydrojasmonate synergist, which is compounded with emamectin benzoate, acetamiprid, and cyantraniliprole, which are widely used to control thrips. The synergistic effect of propyl dihydrojasmonate on these three agents is measured, and a compounding technology system suitable for the control of tobacco thrips is established, providing an innovative solution to address chemical pesticide resistance and ecological risks. To address the above problems, the present invention provides the following technical solutions: A compound insecticide composition containing propyl dihydrojasmonate, wherein the main active ingredients of the compound insecticide composition include propyl dihydrojasmonate and an insecticide.
[0015] Propyl dihydrojasmonate (PDJ) is a synthetic, highly bioactive jasmonic acid derivative. Its molecular structure is highly similar to that of natural jasmonic acid. It possesses stronger permeability, greater stability, and greater bioactivity than jasmonic acid and its methyl ester, making it more readily absorbed by plants and less susceptible to degradation. Propyl dihydrojasmonate can enter plants through foliar application or root absorption, mimicking jasmonic acid signals and activating defense signaling networks, including the MYC2 transcription factor, thereby systemically enhancing plant resistance to insects. Compared to natural jasmonic acid, PDJ exhibits a more sustained induction effect and significantly reduces its inhibitory effect on plant growth.
[0016] In the insecticide compound composition containing propyl dihydrojasmonate as described above, the insecticide is selected from any one of emamectin benzoate, acetamiprid, and cyantraniliprole.
[0017] In the insecticide compound composition containing propyl dihydrojasmonate as described above, the mass ratio of the active ingredient of the propyl dihydrojasmonate to the insecticide is (56-60):(100-1.25). Preferably, the mass ratio of the active ingredient of the propyl dihydrojasmonate to the insecticide is 60:(100-1.25). Optionally, the mass ratio of the active ingredient of the propyl dihydrojasmonate to the insecticide is 60:100, 60:80, 60:50, 60:25, 60:20, 60:12.5, 60:6.25, 60:3.125, 60:1.5625, or 60:1.25.
[0018] The insecticidal compound composition containing propyl dihydrojasmonate as described above, wherein the insecticide is selected from emamectin benzoate, and the mass content ratio of the active ingredients of propyl dihydrojasmonate and emamectin benzoate is (56 - 60):(100 - 1.5625). Preferably, the mass content ratio of the active ingredients of propyl dihydrojasmonate and emamectin benzoate is 60:(100 - 1.5625). Optionally, the mass content ratio of the active ingredients of propyl dihydrojasmonate and emamectin benzoate is 60:100, 60:25, 60:6.25 or 60:1.5625.
[0019] The insecticidal compound composition containing propyl dihydrojasmonate as described above, wherein the insecticide is selected from acetamiprid, and the mass content ratio of the active ingredients of propyl dihydrojasmonate and acetamiprid is (56 - 60):(50 - 3.125). Preferably, the mass content ratio of the active ingredients of propyl dihydrojasmonate and acetamiprid is 60:(50 - 3.125). Optionally, the mass content ratio of the active ingredients of propyl dihydrojasmonate and acetamiprid is 60:50, 60:25, 60:12.5, 60:6.25 or 60:3.125.
[0020] The insecticidal compound composition containing propyl dihydrojasmonate as described above, wherein the insecticide is selected from cyantraniliprole, and the mass content ratio of the active ingredients of propyl dihydrojasmonate and cyantraniliprole is (56 - 60):(80 - 1.25). Preferably, the mass content ratio of the active ingredients of propyl dihydrojasmonate and cyantraniliprole is 60:(5 - 1.25). Optionally, the mass content ratio of the active ingredients of propyl dihydrojasmonate and cyantraniliprole is 60:5 or 60:1.25.
[0021] The insecticidal compound composition containing propyl dihydrojasmonate as described above can be prepared into any agriculturally acceptable preparation dosage form.
[0022] Based on the same inventive concept, the present invention provides an application of the insecticidal compound composition as described above in controlling plant pests in agriculture, forestry and horticulture. The plant is any one of rape, kidney bean, tomato, pepper, cucumber, watermelon, onion, garlic, scallion, cowpea, cotton, tobacco; the pest is any one of thrips, whitefly, aphid, mite, etc. Preferably, the plant is rape or kidney bean, and the pest is thrips. Most preferably, the plant is rape, and the pest is Thrips tabaci.
[0023] The application of the insecticidal compound composition as described above in controlling plant pests in agriculture, forestry and horticulture, wherein the insecticidal compound composition is applied to the pests to be controlled or their growth medium at an effective dose.
[0024] Based on the same inventive concept, the present invention provides an application of the pesticidal compound composition as described above in controlling Thrips tabaci, and the pesticidal compound composition is used for preventing and / or controlling Thrips tabaci pests and their larvae and eggs.
[0025] Compared with the existing technology, the effects and advantages of the present invention are as follows: 1. For the synergistic compound composition containing propyl dihydrojasmonate pesticide provided by the present invention, through indoor toxicity tests, it is found that after propyl dihydrojasmonate is compounded with any one of emamectin benzoate, acetamiprid, and cyantraniliprole, there is an obvious synergistic effect, and propyl dihydrojasmonate can significantly improve the control effect on Thrips tabaci.
[0026] 2. The synergistic compound composition containing propyl dihydrojasmonate pesticide provided by the present invention not only improves the insecticidal efficiency of the pesticide, but also reduces the dosage of the pesticide, which helps to reduce pesticide residues and protect the ecological environment.
[0027] 3. As a plant endogenous hormone, propyl dihydrojasmonate pesticide also has biological activities such as promoting plant growth and enhancing plant stress resistance, which helps to improve the yield and quality of crops. Specific Embodiments
[0028] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the content in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without making creative efforts shall fall within the protection scope of the present invention.
[0029] Unless otherwise defined, all technical and scientific terms used in this specification have the same meaning as commonly understood by those skilled in the technical field to which the present invention belongs. The terms used in the specification of the present invention are only for the purpose of describing specific embodiments and are not used to limit the present invention. The term "and / or" used in this specification includes any and all combinations of one or more of the related listed items.
[0030] The experimental methods used in the following embodiments are all conventional methods unless otherwise specified.
[0031] The materials, reagents, etc. used in the following embodiments can be obtained from commercial channels unless otherwise specified.
[0032] Example 1 Toxicity Test of Propyl Dihydrojasmonate on Thrips tabaci 1. Experimental Raw Materials and Methods: Test insect source: The tobacco thrips population was collected in the greenhouse of Yifengdian Town, Jimo in 2024. It was raised indoors with rapeseed, kidney beans and other vegetables, and the adult thrips were used for the experiment.
[0033] Test agent: Propyl dihydrojasmonate solution with an active ingredient content of 15% (SL, purchased from Qingdao Qingheyuan Agricultural Technology Co., Ltd.
[0034] Chemical treatment: Dihydrojasmonate solution was diluted with sterile water into five concentration gradients (2000 mg / L, 1000 mg / L, 500 mg / L, 250 mg / L, 125 mg / L). Five dihydrojasmonate treatment groups with gradient concentrations and a water control group (CK) were set up.
[0035] Experimental Method: The pesticide was evenly sprayed onto rapeseed plants at a rate of 5 mL per plant. After the leaves dried, they were placed in insectary jars and fed with tobacco thrips. Three replicates were set up for each treatment group, with 15 individuals per replicate, and each insectary jar constituted one replicate. After 72 hours, the mortality of tobacco thrips was counted. Thrips were gently touched with a brush; if they did not move, they were considered dead.
[0036] The mortality rate and adjusted mortality rate were calculated according to the following formula: Mortality rate (%) = (number of dead insects / total number of insects) × 100; Adjusted mortality rate (%) = (mortality rate of treatment group - mortality rate of control group) / (1 - mortality rate of control group) × 100.
[0037] The results are shown in Table 1.
[0038] 2. Experimental results and analysis: Table 1 shows that propyl dihydrojasmonate (PDJ) with an active ingredient content ranging from 500 to 2000 mg / L exhibited very low toxicity against Thrips tabaci. At a PDJ concentration of 2000 mg / L, the mortality rate reached a peak of 26.67%, with a corrected mortality rate of 21.43%. At a PDJ concentration of 500 mg / L, the mortality rate was 8.33%, with a corrected mortality rate of 1.79%. At concentrations of 250 mg / L and 125 mg / L, the mortality rate was no higher than that of the water control. These results indicate that PDJ exhibits a toxic effect against Thrips tabaci only at concentrations of 500 mg / L and above; concentrations below 250 mg / L have no toxic effect. Therefore, in the following examples, a PDJ concentration of 60 mg / L had no toxic effect against Thrips tabaci.
[0039] Table 1 Toxic effect of different concentrations of propyl dihydrojasmonate on Thrips tabaci
[0040] Example 2 Toxicity effect of propyl dihydrojasmonate and emamectin benzoate mixture against Thrips tabaci 1. Experimental raw materials and methods: Test insect source: Thrips tabaci population collected from the greenhouse in Yifengdian Town, Jimo in 2024. They were reared indoors with vegetables such as rape and kidney beans, and adult thrips were used for the experiment.
[0041] Test agents: 5% emamectin benzoate microemulsion (ME, purchased from Huizhou Yinnong Technology Co., Ltd.), 15% propyl dihydrojasmonate solution (SL, purchased from Qingdao Qingheyuan Agricultural Technology Co., Ltd.).
[0042] Agent treatment: Dilute the emamectin benzoate soluble concentrate with water into 4 gradient concentrations (100mg / L, 25mg / L, 6.25mg / L, 1.5625mg / L), and set 4 gradient concentration single-agent treatment groups of emamectin benzoate; at the same time, propyl dihydrojasmonate and 4 gradient concentrations of emamectin benzoate were compounded at the mass content ratio of active ingredients of 60:100, 60:25, 60:6.25, 60:1.5625 respectively, and set 4 treatment groups compounded with propyl dihydrojasmonate with the same gradient concentrations as the single-agent treatment groups of emamectin benzoate. In addition, set 1 clear water control group (CK).
[0043] Experimental method: Spray the agents evenly on rape plants at a dosage of 5 mL / plant. After the leaves are dried, put them into insect-raising bottles to feed Thrips tabaci. Set 3 replicates for each treatment group, with 15 Thrips tabaci in each replicate, and each insect-raising bottle is a replicate. After 72 hours, count the death situation of Thrips tabaci. Gently touch the thrips with a writing brush, and those that do not move are considered dead.
[0044] Calculate the mortality rate, corrected mortality rate and synergistic ratio according to the following formulas: Mortality rate (%) = (number of dead insects / total number of insects) × 100; Corrected mortality rate (%) = (mortality rate of treatment group - mortality rate of clear water control group) / (1 - mortality rate of clear water control group) × 100.
[0045] Synergistic ratio = LC 50 value of emamectin benzoate single agent / LC 50 value of emamectin benzoate single agent after compounding with propyl dihydrojasmonate. A synergistic ratio greater than 1 represents a synergistic effect.
[0046] Data processing: Use POLO Plus and Excel software to calculate the virulence regression equation, correlation coefficient, LC 50 value and 95% confidence interval.
[0047] The results are shown in Tables 2, 3, and 4.
[0048] 2. Experimental results and analysis: Tables 2 and 3 show that the corrected mortality rates of each treatment group of emamectin benzoate compounded with propyl dihydrojasmonate against Thrips tabaci are higher than those of the emamectin benzoate single-agent treatment group. After compounding with propyl dihydrojasmonate, the corrected mortality rates of emamectin benzoate at each concentration against Thrips tabaci are significantly increased.
[0049] Table 2 Toxicity effects of emamectin benzoate at different concentrations on Thrips tabaci
[0050] Table 3 Toxicity effects of emamectin benzoate at different concentrations on Thrips tabaci after compounding with propyl dihydrojasmonate
[0051] Table 4 shows that propyl dihydrojasmonate has an obvious synergistic effect on the control of Thrips tabaci by emamectin benzoate, and the synergistic ratio is 4.543; after compounding with propyl dihydrojasmonate, the LC 50 value of the emamectin benzoate single-agent decreases from 12.895 mg / L to 2.838 mg / L.
[0052] Table 4 Synergistic effect of propyl dihydrojasmonate on the control of Thrips tabaci by emamectin benzoate
[0053] Example 3 Toxicity effects of the compound of propyl dihydrojasmonate and acetamiprid on Thrips tabaci 1. Experimental raw materials and methods: Test insect source: The Thrips tabaci population collected from the greenhouse in Yifengdian Town, Jimo in 2024 was reared indoors with vegetables such as rape and kidney beans, and adult thrips were used for the experiment.
[0054] Test agents: Acetamiprid soluble concentrate (SL) with an active ingredient content of 20% (purchased from Jiangxi Zhongxun Agrochemical Co., Ltd.), and propyl dihydrojasmonate liquid agent (SL) with an active ingredient content of 15% (purchased from Qingdao Qingheyuan Agricultural Technology Co., Ltd.).
[0055] Agent treatment: The soluble concentrate of acetamiprid was diluted with clear water into 5 gradient concentrations (50 mg / L, 25 mg / L, 12.5 mg / L, 6.25 mg / L, 3.125 mg / L), and 5 treatment groups of acetamiprid single agent with 5 gradient concentrations were set; at the same time, propyl dihydrojasmonate and acetamiprid with 5 gradient concentrations were compounded at the mass content ratio of active ingredients of 60:50, 60:25, 60:12.5, 60:6.25, 60:3.125 respectively, and 5 treatment groups compounded with propyl dihydrojasmonate and having the same 5 gradient concentrations as the acetamiprid single agent treatment groups were set. In addition, 1 clear water control group (CK) was set.
[0056] Experimental method: The agent was evenly sprayed on the rape plants, and the spraying dosage was 5 mL / plant. After the leaves were dried, they were put into an insect-raising bottle to feed Thrips tabaci. 3 replicates were set for each treatment group, with 15 Thrips tabaci in each replicate, and each insect-raising bottle was a replicate. After 72 hours, the death situation of Thrips tabaci was counted. The thrips were gently touched with a writing brush, and if they did not move, they were regarded as dead.
[0057] The mortality rate, corrected mortality rate and synergistic ratio were calculated according to the following formulas: Mortality rate (%) = (number of dead insects / total number of insects) × 100; Corrected mortality rate (%) = (mortality rate of treatment group - mortality rate of clear water control group) / (1 - mortality rate of clear water control group) × 100.
[0058] Synergistic ratio = LC 50 value of acetamiprid single agent / LC 50 value of acetamiprid single agent after compounding with propyl dihydrojasmonate. A synergistic ratio greater than 1 represents a synergistic effect.
[0059] Data processing: The POLO Plus and Excel software were used to calculate the virulence regression equation, correlation coefficient, LC 50 value and 95% confidence interval.
[0060] The results are shown in Table 5, Table 6 and Table 7.
[0061] 2. Experimental results and analysis: Table 5 and Table 6 show that the corrected mortality rates of each treatment group of the compound of acetamiprid and propyl dihydrojasmonate against Thrips tabaci are higher than those of the acetamiprid single agent treatment group. After compounding with propyl dihydrojasmonate, the corrected mortality rate of acetamiprid at a concentration of 50 mg / L against Thrips tabaci increased from 97.56% to 100.00%. As the concentration of acetamiprid decreased, the synergistic effect of propyl dihydrojasmonate became more obvious. After compounding with propyl dihydrojasmonate, the corrected mortality rate of acetamiprid at a concentration of 3.125 mg / L against Thrips tabaci increased from 7.32% to 15.38%.
[0062] Table 5 Toxicity effect of acetamiprid single agent with different concentrations on Thrips tabaci
[0063] Toxicity effect of acetamiprid at different concentrations after compounding with propyl dihydrojasmonate on Thrips tabaci
[0064] Table 7 shows that propyl dihydrojasmonate has a synergistic effect on the control of Thrips tabaci by acetamiprid, and the synergistic ratio is 1.208; after compounding with propyl dihydrojasmonate, the LC 50 value of acetamiprid single agent decreased from 7.541 mg / L to 6.243 mg / L.
[0065] Table 7 Synergistic effect of propyl dihydrojasmonate on the control of Thrips tabaci by acetamiprid
[0066] Example 4 Toxicity effect of the compounding of propyl dihydrojasmonate and cyantraniliprole on Thrips tabaci 1. Experimental raw materials and methods: Test insect source: Thrips tabaci population collected from the greenhouse in Yifengdian Town, Jimo in 2024, reared indoors with vegetables such as rape and kidney beans, and adult thrips were used for the test.
[0067] Test agents: Cyantraniliprole dispersible oil suspension (OD) with an active ingredient content of 10% (purchased from FMC Corporation, USA), and propyl dihydrojasmonate liquid agent (SL) with an active ingredient content of 15% (purchased from Qingdao Qingheyuan Agricultural Technology Co., Ltd.).
[0068] Agent treatment: Dilute the cyantraniliprole dispersible oil suspension with water to 4 concentration gradients (80 mg / L, 20 mg / L, 5 mg / L, 1.25 mg / L), and set 4 gradient concentration single-agent treatment groups of cyantraniliprole; at the same time, compound propyl dihydrojasmonate with 4 gradient concentrations of cyantraniliprole at the mass ratio of active ingredients of 60:80, 60:20, 60:5, 60:1.25 respectively, set 4 treatment groups compounded with propyl dihydrojasmonate with the same gradient concentrations as the single-agent treatment groups of cyantraniliprole, and set another 1 water control group (CK).
[0069] Experimental method: Spray the agent evenly on rape plants, with a spraying dosage of 5 mL / plant. After the leaves are dried, put them into the insect-raising bottle to feed Thrips tabaci. Each treatment group is set with 3 replicates, 15 Thrips tabaci in each replicate, and each insect-raising bottle is a replicate. After 72 hours, count the death situation of Thrips tabaci. Gently touch the thrips with a writing brush, and if it doesn't move, it is regarded as dead.
[0070] Calculate the mortality rate, corrected mortality rate and synergistic ratio according to the following formulas: Mortality rate (%) = (number of dead insects / total number of insects) × 100; Corrected mortality rate (%) = (mortality rate of treatment group - mortality rate of clear water control group) / (1 - mortality rate of clear water control group) × 100.
[0071] Synergistic ratio = LC 50 value of the single agent of cyantraniliprole / LC 50 value of the single agent of cyantraniliprole after being compounded with propyl dihydrojasmonate. A synergistic ratio greater than 1 represents a synergistic effect.
[0072] Data processing: Use POLO Plus and Excel software to calculate the virulence regression equation, correlation coefficient, LC 50 value and 95% confidence interval.
[0073] The results are shown in Table 8, Table 9, and Table 10.
[0074] 2. Experimental results and analysis: Tables 8 and 9 show that when the concentration of cyantraniliprole is 80 mg / L and 20 mg / L, the corrected mortality rate of the compound treatment group of cyantraniliprole and propyl dihydrojasmonate against Thrips tabaci is lower than that of the single agent treatment group of cyantraniliprole; while when the concentration of cyantraniliprole is 5 mg / L and 1.25 mg / L, the corrected mortality rate of the compound treatment group of cyantraniliprole and propyl dihydrojasmonate against Thrips tabaci is higher than that of the single agent treatment group of cyantraniliprole. Especially when the concentration of cyantraniliprole is 1.25 mg / L, the corrected mortality rate of the compound treatment group of cyantraniliprole and propyl dihydrojasmonate against Thrips tabaci is increased by 24.39% compared with the single agent treatment group of cyantraniliprole.
[0075] Table 8 Toxicity effects of single agents of cyantraniliprole at different concentrations against Thrips tabaci
[0076] Table 9 Toxicity effects of cyantraniliprole at different concentrations after being compounded with propyl dihydrojasmonate against Thrips tabaci
[0077] Table 10 shows that propyl dihydrojasmonate has a synergistic effect on the control of Thrips tabaci by cyantraniliprole, and the synergistic ratio is 1.171; after being compounded with propyl dihydrojasmonate, the LC 50 value of the single agent of cyantraniliprole drops from 5.957 mg / L to 5.088 mg / L.
[0078] Table 10 Synergistic effect of propyl dihydrojasmonate on the control of Thrips tabaci by cyantraniliprole
[0079] In summary, as can be seen from the above embodiments, after the propyl dihydrojasmonate (PDJ) insecticidal synergist of the present invention is compounded with various insecticides, the insecticidal effect on Thrips tabaci can be improved. This synergist not only has a broad-spectrum synergistic effect, but also can reduce the dosage of pesticides, lower pesticide residues, and protect the ecological environment. Therefore, the present invention has broad application prospects in the field of agricultural pest control.
[0080] It should be noted that the specific embodiments are only relatively representative examples of the present invention. Obviously, the technical solutions of the present invention are not limited to the above embodiments and there can be many variations. Those of ordinary skill in the art who obtain the present invention clearly disclosed or without any doubt according to the written description of the document shall be considered as the scope to be protected by this patent.
Claims
1. An insecticidal compound composition containing propyl dihydrojasmonate, characterized in that, The main active ingredients of the insecticidal compound composition include propyl dihydrojasmonate and an insecticide.
2. The insecticidal compound composition containing propyl dihydrojasmonate according to claim 1, characterized in that, The insecticide is selected from any one of emamectin benzoate, acetamiprid, and cyantraniliprole.
3. The insecticidal compound composition containing propyl dihydrojasmonate according to claim 1, characterized in that, The mass content ratio of the active ingredients of the propyl dihydrojasmonate and the insecticide is (56-60):(100-1.25).
4. The insecticidal compound composition containing propyl dihydrojasmonate according to claim 2, wherein, The insecticide is selected from emamectin benzoate, and the mass content ratio of the active ingredients of the propyl dihydrojasmonate and emamectin benzoate is (56-60):(100-1.5625).
5. The insecticidal compound composition containing propyl dihydrojasmonate according to claim 2, characterized in that, The insecticide is selected from acetamiprid, and the mass content ratio of the active ingredients of the propyl dihydrojasmonate and acetamiprid is (56-60):(50-3.125).
6. The insecticidal compound composition containing propyl dihydrojasmonate according to claim 2, characterized in that, The insecticide is selected from cyantraniliprole, and the mass content ratio of the active ingredients of the propyl dihydrojasmonate and cyantraniliprole is (56-60):(80-1.25).
7. The insecticidal compound composition containing propyl dihydrojasmonate according to claim 1, wherein the insecticidal compound composition can be prepared into any agriculturally acceptable preparation dosage form.
8. Use of the insecticidal compound composition according to any one of claims 1-7 in controlling plant pests in agriculture, forestry and horticulture, characterized in that, The plant is any one of rape, kidney bean, tomato, chili pepper, cucumber, watermelon, onion, garlic, scallion, cowpea, cotton, and tobacco; the pest is any one of thrips, whitefly, aphid, and mite.
9. Use of the insecticidal compound composition according to claim 8 in controlling plant pests in agriculture, forestry and horticulture, characterized in that, The insecticidal compound composition is applied to the pest to be controlled or its growth medium at an effective dose.
10. Use of the insecticidal compound composition according to any one of claims 1-7 in controlling Thrips tabaci, characterized in that, Use of the insecticidal compound composition for preventing and / or controlling tobacco thrips pests and their larvae and eggs.