Whitening composition and preparation method thereof

Abstract

The invention discloses a whitening composition and a preparation method thereof, and belongs to the technical field of functional food, the whitening composition comprises the following components by mass: 20.7-40.7 parts of astaxanthin oil, 45-65 parts of grape seed oil, 5-7 parts of gamma-aminobutyric acid, 4-6 parts of purified water, 1.4-1.8 parts of a grape seed extract (resveratrol), 0.1-0.3 part of a white tomato extract, 0.6-1 part of a polygonatum odoratum extract, and 0.6-0.8 part of a mulberry leaf extract. The whitening composition disclosed by the invention has a good melanin inhibition effect.

Description

Technical Field

[0001] The present invention belongs to the technical field of functional foods, and specifically relates to a whitening composition and a preparation method thereof. Background Art

[0002] With the development of society, and people's yearning and pursuit of a better life, beauty-loving has become an increasingly important pursuit in life, and whitening is an important goal of becoming beautiful.

[0003] Tyrosinase plays an important role in the formation of melanin. Tyrosinase (TYR) is a copper-containing metalloenzyme with a highly conserved binuclear copper-binding domain. Each copper ion is connected to three histidine residues through coordination bonds to form an active site with a specific structure. The binding of dioxygen to the two copper ions enables TYR to exist in three different states: oxidized, reduced, and deoxygenated, thereby exerting a catalytic effect. TYR plays an important role in pigment production. It is the rate-limiting enzyme for synthesizing melanin, and inhibiting the activity of TYR is important for the adjuvant treatment of melanoma and most pigmentations, which has also promoted the development of whitening cosmetics.

[0004] In the field of whitening cosmetics, the commonly used ingredients that can inhibit the activity of tyrosinase mainly include kojic acid, arbutin, vitamin C, tropolone, hydroquinone, etc., such as kojic acid, arbutin, and hydroquinone. However, most of these whitening additives have certain side effects. For example, arbutin may be a precursor of hydroquinone, which is toxic and side-effect to the skin; although kojic acid has a strong inhibitory effect on tyrosinase, it is unstable and may be carcinogenic after long-term use; the skin absorbability of vitamin C and its derivatives is poor, and it is only effective at high concentrations, etc. Summary of the Invention

[0005] In order to overcome the problems existing in the prior art, the present invention provides a whitening composition and a preparation method thereof.

[0006] To achieve the above object, the present invention is realized by the following technical solutions: A whitening composition comprising the following components in parts by mass: 20.7 - 40.7 parts of astaxanthin oil, 45 - 65 parts of grape seed oil, 5 - 7 parts of γ-aminobutyric acid, 4 - 6 parts of purified water, 1.4 - 1.8 parts of grape seed extract, 0.1 - 0.3 parts of white tomato extract, 0.6 - 1 part of polygonatum odoratum extract, and 0.6 - 0.8 part of mulberry leaf extract.

[0007] Further, it comprises the following components in parts by mass: 29.7 parts of astaxanthin oil, 55 parts of grape seed oil, 6 parts of γ-aminobutyric acid, 5 parts of purified water, 1.6 parts of grape seed extract, 0.2 part of white tomato extract, 0.8 part of polygonatum odoratum extract, and 0.7 part of mulberry leaf extract.

[0008] Furthermore, it contains components with the following mass fractions: 20.7 parts of astaxanthin oil, 45 parts of grape seed oil, 5 parts of γ-aminobutyric acid, 4 parts of purified water, 1.4 parts of grape seed extract, 0.1 part of white tomato extract, 0.6 part of polygonatum odoratum extract, and 0.6 part of mulberry leaf extract.

[0009] Furthermore, 40.7 parts of astaxanthin oil, 65 parts of grape seed oil, 7 parts of γ-aminobutyric acid, 6 parts of purified water, 1.8 parts of grape seed extract, 0.3 part of white tomato extract, 1 part of polygonatum odoratum extract, and 0.8 part of mulberry leaf extract.

[0010] The present invention also provides a preparation method of the above-mentioned whitening composition, which comprises the following steps: S1, After mixing astaxanthin oil and grape seed oil, stir evenly to obtain an oil phase; S2, Add γ-aminobutyric acid, grape seed extract, white tomato extract, polygonatum odoratum extract, and mulberry leaf extract to purified water and stir to disperse evenly to obtain an aqueous phase; S3, Mix the aqueous phase and the oil phase, stir and disperse evenly to obtain a suspension. The suspension is first treated with a high-speed dispersion homogenizer and then with an ultrasonic cell disrupter; When using the high-speed dispersion homogenizer: at 10000r / min - 12000r / min, perform high-speed shear dispersion for 9 - 15min; When using the ultrasonic cell disrupter: at a power of 100W, perform ultrasonic dispersion for 2min, and then at a power of 200W, perform ultrasonic dispersion for 4min, and select the mode of working for 1s and intermittent for 1s to obtain the whitening composition.

[0011] The present invention also provides a capsule of the whitening composition. The inclusion of the capsule is the above-mentioned whitening composition, and the capsule wall of the capsule contains components with the following mass fractions: 38.6% of gelatin, 38.6% of purified water, and 22.8% of glycerol Through the above technical solutions, the present invention can at least achieve the following beneficial effects: The whitening composition of the present invention has a good melanin inhibition effect. Detailed Embodiments

[0012] Unless otherwise specified, the materials and reagents used in the present invention are commercially available.

[0013] In the present invention: Astaxanthin oil and purified water are self-extracted / prepared by our company (Yunnan Green A Bio-Engineering Co., Ltd.), and the astaxanthin oil contains 5% of astaxanthin.

[0014] Grape seed oil is purchased from Heze Zhonghe Jianyuan Biotechnology Co., Ltd.; γ-aminobutyric acid is purchased from Shandong Kangyou Biotechnology Co., Ltd. and passes through an 80-mesh sieve; The grape seed extract was purchased from Bozhou Xier Chinese Herbal Medicine Co., Ltd.; The white tomato extract was purchased from Glide Technology (Shanghai) Co., Ltd.; The polygonatum odoratum extract and the mulberry leaf extract were purchased from Shanyang Lianfeng Biotechnology Co., Ltd. (both are water extracts with a specification of 20:1); The gelatin was purchased from Kunming Sigui Economic and Trade Co., Ltd.; The glycerol was purchased from Jiangxi Ipsen Pharmaceutical Co., Ltd. Example 1

[0015] A whitening composition, comprising the following components in parts by mass: 29.7 parts of astaxanthin oil, 55 parts of grape seed oil, 6 parts of γ-aminobutyric acid, 5 parts of purified water, 1.6 parts of grape seed extract, 0.2 part of white tomato extract, 0.8 part of polygonatum odoratum extract, and 0.7 part of mulberry leaf extract.

[0016] The preparation method of the above whitening composition comprises the following steps: S1, After mixing the astaxanthin oil and the grape seed oil, stir evenly to obtain an oil phase; S2, Add γ-aminobutyric acid, grape seed extract, white tomato extract, polygonatum odoratum extract, and mulberry leaf extract to the purified water and stir to disperse evenly to obtain an aqueous phase; S3, Mix the aqueous phase and the oil phase, stir and disperse evenly to obtain a suspension. The suspension is first treated with a high-speed dispersion homogenizer and then with an ultrasonic cell disruptor; When using the high-speed dispersion homogenizer: At 11000 r / min, perform high-speed shear dispersion for 12 min; When using the ultrasonic cell disruptor: At a power of 100 W, perform ultrasonic dispersion for 2 min, and then at a power of 200 W, perform ultrasonic dispersion for 4 min, and select the mode of working for 1 s and intermittent for 1 s to obtain the whitening composition. Example 2

[0017] A whitening composition, comprising the following components in mass fractions: 20.7 parts of astaxanthin oil, 45 parts of grape seed oil, 5 parts of γ-aminobutyric acid, 4 parts of purified water, 1.4 parts of grape seed extract, 0.1 part of white tomato extract, 0.6 part of polygonatum odoratum extract, and 0.6 part of mulberry leaf extract.

[0018] The preparation method of the above whitening composition comprises the following steps: S1, After mixing the astaxanthin oil and the grape seed oil, stir evenly to obtain an oil phase; S2, Add γ-aminobutyric acid, grape seed extract, white tomato extract, polygonatum odoratum extract, and mulberry leaf extract to the purified water and stir to disperse evenly to obtain an aqueous phase; S3. Mix the aqueous phase and the oil phase, stir and disperse them evenly to obtain a suspension. First, process the suspension with a high-speed dispersing homogenizer, and then process it with an ultrasonic cell disruptor. When using the high-speed dispersing homogenizer: at 10,000 r / min, perform high-speed shear dispersion for 9 min. When using the ultrasonic cell disruptor: at a power of 100 W, perform ultrasonic dispersion for 2 min, and then at a power of 200 W, perform ultrasonic dispersion for 4 min, and select the mode of working for 1 s and intermittent for 1 s to obtain the whitening composition. Example 3

[0019] A whitening composition, comprising the following components in parts by mass: 40.7 parts of astaxanthin oil, 65 parts of grape seed oil, 7 parts of γ-aminobutyric acid, 6 parts of purified water, 1.8 parts of grape seed extract, 0.3 part of white tomato extract, 1 part of polygonatum odoratum extract, and 0.8 part of mulberry leaf extract.

[0020] The preparation method of the above-mentioned whitening composition comprises the following steps: S1. After mixing the astaxanthin oil and the grape seed oil, stir evenly to obtain an oil phase; S2. Add γ-aminobutyric acid, grape seed extract, white tomato extract, polygonatum odoratum extract, and mulberry leaf extract to the purified water, stir and disperse evenly to obtain an aqueous phase; S3. Mix the aqueous phase and the oil phase, stir and disperse evenly to obtain a suspension. First, process the suspension with a high-speed dispersing homogenizer, and then process it with an ultrasonic cell disruptor. When using the high-speed dispersing homogenizer: at 12,000 r / min, perform high-speed shear dispersion for 152 min. When using the ultrasonic cell disruptor: at a power of 100 W, perform ultrasonic dispersion for 2 min, and then at a power of 200 W, perform ultrasonic dispersion for 4 min, and select the mode of working for 1 s and intermittent for 1 s to obtain the whitening composition. Example 4

[0021] A capsule of a whitening composition, the content of which is the whitening composition of Example 1. The capsule wall of the capsule comprises the following components in mass fractions: 38.6% of gelatin, 38.6% of purified water, and 22.8% of glycerol.

[0022] The preparation method of the above-mentioned capsule comprises the following steps: S1. After preparing the whitening composition, perform vacuum defoaming to obtain the capsule content; S2. Heat the purified water to 80°C in the sol tank, add glycerol, mix well, then heat to 80°C again. Open the vacuum pump, evacuate the air, stir at a speed of 140 rpm / min, suck gelatin into the sol tank with the feed pipe. After completion, close the feed valve and the vacuum pump, and continue stirring for 20 minutes. Open the vacuum pump, evacuate the air to remove bubbles, continue stirring at a speed of 140 rpm / min. When the temperature drops below 60°C, stop the vacuum. Heat the mixture to 62°C for standby to obtain the wall material of the capsule. S3. Encapsulate the core material and the wall material of the capsule with a soft capsule encapsulating machine, and control the wall thickness of the capsule to be 0.7 - 0.9 mm. Comparative Example 1

[0023] The difference between Comparative Example 1 and Example 1 is that it does not contain polygonatum odoratum extract. Comparative Example 2

[0024] The difference between Comparative Example 2 and Example 1 is that it does not contain morus alba leaf extract. Comparative Example 3

[0025] The difference between Comparative Example 3 and Example 1 is that it does not contain polygonatum odoratum extract and morus alba leaf extract. Test Example

[0026] 1. Melanin Inhibition Test Detect according to the "Cosmetics Melanin Inhibition Test - Zebrafish Embryo Test Method".

[0027] Dilute the whitening compositions of Example 1 - 3 and Comparative Examples 1 - 3 with purified water and a co - solvent (dimethyl sulfoxide) to 100 mg / L to obtain the test sample solution; the total content of DMSO is 0.08 wt% of the test sample solution.

[0028] Fish embryo culture medium: Prepared by dissolving 2940 mg of anhydrous calcium chloride, 1240 mg of magnesium sulfate heptahydrate, 630 mg of sodium bicarbonate, and 55 mg of potassium chloride in 10 L of water.

[0029] Test group: Expose 24 8 - hour - old zebrafish embryos to the test sample solution. Blank group: Expose 24 8 - hour - old zebrafish embryos to the fish embryo culture medium. Positive control group: Expose 24 8 - hour - old zebrafish embryos to a 30 mg / L phenylthiourea aqueous solution. After exposing in a constant - temperature incubator at 28°C for 48 hours, detect the melanin signal intensity of the fish embryos and conduct statistical analysis. Make 3 parallel samples for each. The calculation formula for the melanin inhibition rate is as follows: Melanin inhibition rate = (average value of "average signal intensity" in blank control group - average value of "average signal intensity" in sample solution) / (average value of "average signal intensity" in blank control group - average value of "average signal intensity" in positive control group) × 100%.

[0030] The statistical results are shown in Table 1 below.

[0031] Table 1 Statistical table of melanin inhibition rate of samples in Examples 1 - 3 and Comparative Examples 1 - 3 Sample Melanin inhibition rate of the test group / % Example 1 26.84 Example 2 25.39 Example 3 27.05 Comparative Example 1 21.17 Comparative Example 2 22.56 Comparative Example 3 19.87 As can be seen from Table 1 above, compared with Comparative Examples 1 - 3, the melanin inhibition rate of the whitening compositions in Examples 1 - 3 is higher.

[0032] 2. Tyrosinase activity inhibition test Prepare tyrosinase solution with pH 6.8, 50 mmol / L PBS buffer solution, and prepare 8 g / L composition solution with 40% (v / v) ethanol aqueous solution; in a 96 - well plate, successively add 40 μL of 0.1 mg / ml tyrosine, 80 μL of PBS buffer solution, 50 μL of composition solution, and 50 μL of 60 U / ml tyrosinase solution. After incubating at 37 °C for 30 min, detect with a microplate reader at a wavelength of 475 nm. Using PBS buffer solution as the blank group, each sample is made into 3 parallel samples. The calculation formula for the inhibition rate of tyrosinase activity is as follows: Tyrosinase activity inhibition rate (%) = [(A1 - A2) - (A3 - A4)] / (A1 - A2) × 100%; In the formula, A1 is the absorbance after incubation of the blank solution, A2 is the absorbance before incubation of the blank solution, A3 is the absorbance after incubation of the composition solution, and A4 is the absorbance before incubation of the composition solution. The detection results are shown in Table 2 below.

[0033] Table 2 Statistical table of inhibition rate of tyrosinase activity of samples in Examples 1 - 3 and Comparative Examples 1 - 3 Sample Inhibition rate of tyrosinase activity / % Example 1 85.34 Example 2 84.65 Example 3 85.71 Comparative Example 1 76.25 Comparative Example 2 79.58 Comparative Example 3 71.24 As can be seen from Table 2 above, the whitening composition of the present application has a good inhibitory effect on tyrosinase activity.

[0034] Finally, it should be noted that the above - mentioned preferred embodiments are only used to illustrate the technical solutions of the present invention and are not restrictive. Although the present invention has been described in detail through the above - mentioned preferred embodiments, those skilled in the art should understand that various changes can be made in form and details without departing from the scope defined by the claims of the present invention.

Claims

1. A whitening composition, characterized in that: It contains components in the following parts by mass: 20.7 - 40.7 parts of astaxanthin oil, 45 - 65 parts of grape seed oil, 5 - 7 parts of γ-aminobutyric acid, 4 - 6 parts of purified water, 1.4 - 1.8 parts of grape seed extract, 0.1 - 0.3 parts of white tomato extract, 0.6 - 1 part of polygonatum extract, 0.6 - 0.8 parts of mulberry leaf extract.

2. The whitening composition according to claim 1, characterized in that: It contains components in the following parts by mass: 29.7 parts of astaxanthin oil, 55 parts of grape seed oil, 6 parts of γ-aminobutyric acid, 5 parts of purified water, 1.6 parts of grape seed extract, 0.2 parts of white tomato extract, 0.8 parts of polygonatum extract, 0.7 parts of mulberry leaf extract.

3. The whitening composition according to claim 1, wherein: It contains components in the following parts by mass: 20.7 parts of astaxanthin oil, 45 parts of grape seed oil, 5 parts of γ-aminobutyric acid, 4 parts of purified water, 1.4 parts of grape seed extract, 0.1 parts of white tomato extract, 0.6 parts of polygonatum extract, 0.6 parts of mulberry leaf extract.

4. A whitening composition according to claim 1, characterized in that: It contains components in the following parts by mass: 40.7 parts of astaxanthin oil, 65 parts of grape seed oil, 7 parts of γ-aminobutyric acid, 6 parts of purified water, 1.8 parts of grape seed extract, 0.3 parts of white tomato extract, 1 part of polygonatum extract, 0.8 parts of mulberry leaf extract.

5. A method for preparing a whitening composition according to any one of claims 1 or 4, characterized in that: It includes the following steps: S1. After mixing astaxanthin oil and grape seed oil, stir evenly to obtain an oil phase. S2. Add γ-aminobutyric acid, grape seed extract, white tomato extract, polygonatum extract and mulberry leaf extract into purified water and stir to disperse evenly to obtain an aqueous phase. S3. Mix the aqueous phase and the oil phase, stir and disperse evenly to obtain a suspension. The suspension is first treated with a high-speed dispersion homogenizer and then with an ultrasonic cell disruptor. When using the high-speed dispersion homogenizer: at 10000 r / min - 12000 r / min, perform high-speed shearing dispersion for 9 - 15 min. When using the ultrasonic cell disruptor: at a power of 100 W, perform ultrasonic dispersion for 2 min, and then at a power of 200 W, perform ultrasonic dispersion for 4 min, and select the mode of working for 1 s and intermittent for 1 s to obtain the whitening composition.

6. A whitening composition in capsule form, characterized in that: The inclusion of the capsule is the whitening composition according to any one of claims 1 - 4. The capsule wall of the capsule contains components in the following parts by mass: 38.6% of gelatin, 38.6% of purified water and 22.8% of glycerol.