Dietary fiber anti-obesity and lactone-reducing composition as well as preparation method and application thereof

Abstract

The invention provides a dietary fiber anti-obesity and internal fat-reducing composition and a preparation method and application thereof, and the dietary fiber anti-obesity and internal fat-reducing composition comprises the following components in percentage by mass: a metabolic regulation formula which comprises 0.8-1.2% of mulberry leaf extract, 0.3-0.5% of potato extract, 0.2-0.4% of gamma-aminobutyric acid microcapsules and 4.0-6.0% of isomaltooligosacharide; the water management formula comprises 0.5 to 1.0 percent of opuntia ficus-indica powder and 0.2 to 0.4 percent of fructus gardeniae powder; the auxiliary regulation formula comprises the following components in percentage by weight: 2.0 to 4.0 percent of fructo-oligosaccharide, 0.05 to 0.15 percent of sucralose, 0.10 to 0.30 percent of xanthan gum and 0.05 to 0.15 percent of sodium citrate; and the nutrition fortification formula comprises 0.1-0.3% of taurine and 0.5-1.0% of a compound nutrition fortifier, and the compound nutrition fortifier contains vitamin B6 / B12, magnesium, iron and zinc. By constructing a fiber barrier-enzymolysis network-targeted metabolism three-in-one system, accurate reduction and control of visceral fat are achieved, fat oxidation combustion can be promoted, fat decomposition can be accelerated, fat accumulation can be reduced, and the method can be used for preparation of liquid beverages, preparation of powdery electuary and preparation of far infrared bagged powder solid beverages and has good application prospects. And the application scenarios are greatly increased.

Description

Technical Field

[0001] The present invention relates to the technical field of beverages, in particular to a dietary fiber anti-obesity and fat-reducing composition, a preparation method and application thereof. Background Art

[0002] Exercise for weight loss, medication for weight loss, and meal replacement for fat loss are three popular ways to lose fat and slim down in modern times, and they are becoming increasingly popular.

[0003] As the global incidence of obesity and related metabolic syndrome continues to rise year by year, intervention methods targeting visceral fat accumulation have become a research hotspot. Existing commercially available fat-reducing compositions have the following major technical deficiencies: Dietary fiber has a single function: Conventional soluble dietary fiber (such as inulin and oligofructose) can increase satiety in the short term, but it can easily cause intestinal gas production and cannot form a long-term gastrointestinal barrier; Inefficient fat metabolism: The fat decomposition mechanism that relies on a single ingredient (such as L-carnitine) has a mitochondrial transport bottleneck. Clinical trials have shown that its fat oxidation rate is only 12.5±1.8 mg / h. Insufficient targeting of visceral fat: Traditional fat-reducing ingredients lack the synergistic regulation of white adipose tissue browning and beige fat activation, resulting in a visceral fat reduction rate of less than 20%; Metabolic regulation imbalance: The multi-dimensional regulatory network of "fat decomposition-energy metabolism-intestinal flora" has not been established, which can easily lead to ketone body accumulation and intestinal microecological disorder.

[0004] Although the existing technology has introduced far-infrared activation and diglyceride emulsion technology, it still has the following limitations: It does not combine with dietary fiber matrix to build a physical barrier to the gastrointestinal tract; it lacks a dual inhibitory mechanism for α-amylase and lipase; and it does not achieve targeted release of visceral fat and precise regulation of metabolic pathways. Summary of the Invention

[0005] In order to solve the above technical problems, the present invention proposes a dietary fiber anti-obesity and lactone-reducing composition, a preparation method and application thereof, and realizes precise reduction and control of visceral fat by constructing a "fiber barrier-enzymatic network-targeted metabolism" three-in-one system.

[0006] In order to solve the above technical problems, the technical solutions of the present invention are as follows: In a first aspect, a dietary fiber anti-obesity and lactone-reducing composition comprises, by mass percentage,: Metabolic regulation formula, including mulberry leaf extract 0.8-1.2%, potato extract 0.3-0.5%, γ-aminobutyric acid microcapsules 0.2-0.4%, and isomaltooligosaccharide 4.0-6.0%; Moisture management formula, including 0.5-1.0% prickly pear cactus powder and 0.2-0.4% gardenia powder; Auxiliary regulating formula, including fructooligosaccharide 2.0-4.0%, sucralose 0.05-0.15%, xanthan gum 0.10-0.30%, sodium citrate 0.05-0.15%; Nutritional fortification formula, including taurine 0.1-0.3%, compound nutritional fortifier 0.5-1.0%, and nutritional fortifier containing vitamin B6 / B12, magnesium, iron and zinc; Dietary fiber matrix formula, including resistant dextrin 6.5-8.5%, oat beta-glucan 3.5-4.5%, konjac glucomannan 2.0-3.0%; Fat-targeted decomposition formula, including 3.0-3.5% diglyceride emulsion and 0.5-0.8% L-carnitine tartrate; Visceral fat suppression formula, including green tea EGCG nanoliposomes 1.2-1.8% and white kidney bean α-AI extract 0.4-0.6%.

[0007] Furthermore, the diglyceride emulsion adopts a double-layer phospholipid coating structure, the inner layer is diglyceride oil, the purity of the diglyceride oil is ≥95%, and the outer layer is a composite wall material of gellan gum or gum arabic, and triggers colon-targeted release when the pH is ≤5.5; The L-carnitine tartrate is loaded via a sustained-release microsphere carrier, the microsphere particle size is 200-500 nm, and the embedding rate is ≥85%.

[0008] Furthermore, the resistant dextrin is an enzymatically modified product with an average degree of polymerization DP of 12 to 18 and a water holding capacity of ≥8 g / g; The oat beta-glucan has a molecular weight of 800,000 to 1.2 million Daltons and a water solubility of ≥98%.

[0009] Furthermore, the green tea EGCG nanoliposomes have a particle size of 50-100 nm and an encapsulation efficiency of ≥90%, and are prepared by an ethanol injection method; The white kidney bean α-AI extract is extracted and purified by gradient cooling, the gradient cooling range is -10°C→25°C, and the α-amylase inhibition activity unit is ≥2000GU / g.

[0010] Furthermore, the konjac glucomannan is a high-viscosity improved type with a viscosity of ≥20,000 mPa·s (2% aqueous solution); The γ-aminobutyric acid microcapsules use a composite wall material, which is gelatin or gum arabic, and the sustained-release period is ≥8h.

[0011] In a second aspect, a method for preparing a dietary fiber anti-obesity and lactone-reducing composition comprises the following steps: S1: Diglyceride oil and gellan gum / gum arabic were mixed in a mass ratio of 3:1 and subjected to high-pressure homogenization to prepare double-layer microcapsules. The high-pressure homogenization pressure was 120 MPa and the number of cycles was 3; S2: Disperse L-carnitine tartrate in maltodextrin solution and spray dry to prepare sustained-release microspheres; S3: Resistant dextrin, oat β-glucan and inulin are mixed in proportion and dispersed with the aid of ultrasound to form a fiber network, wherein the ultrasound frequency of the ultrasound-assisted dispersion is 40 kHz and the power is 200 W; S4: Mulberry leaf extract, potato extract, green tea EGCG and phosphatidylcholine were mixed in a mass ratio of 2:1:1:4 and nanoliposomes were prepared by ethanol injection method; S5: mixing the white kidney bean extract and the konjac glucomannan solution in a volume ratio of 3:1, and filtering and purifying the mixture through a ceramic membrane, wherein the pore size of the ceramic membrane is 0.2 μm; S6: After mixing the above components with taurine, compound nutritional enhancers and other auxiliary materials in proportion, spray drying or freeze drying is carried out to prepare a composition.

[0012] Furthermore, the high-pressure homogenization temperature in step S1 is controlled at 45-55° C. to avoid oxidation of diglycerides.

[0013] Furthermore, the ethanol concentration in step S4 is 75% (v / v), and the injection speed is 5 mL / min.

[0014] In a third aspect, a dietary fiber anti-obesity and lactone-reducing composition is used in any one or more of powdered granules, liquid beverages, and far-infrared bagged powdered solid beverages.

[0015] The above solution of the present invention includes at least the following beneficial effects: The present invention combines a diglyceride emulsion with L-carnitine tartrate to form a "fat decomposition engine," achieving a fat oxidation rate of 28.7±3.1 mg / h (p<0.01), a 129% improvement over existing technologies. The visceral fat-targeted formulation reduces visceral fat area by 34.2±4.7% (6 weeks), outperforming the 8-week effect of the traditional regimen. The resistant dextrin-oat β-glucan composite fiber network forms a gastric gel barrier with a water-holding capacity of ≥8g / g and a sustained feeling of fullness of 6.8±1.1 hours. The modified konjac glucomannan has a viscosity of ≥20,000 mPa·s, extending intestinal residence time by 2 times and reducing postprandial blood glucose response by 35%. GABA microcapsules and isomaltooligosaccharides synergistically activate the AMPK pathway, increasing fat decomposition efficiency by 40% while inhibiting gluconeogenesis. The far-infrared activated bottle promotes the proliferation of intestinal probiotics and improves intestinal permeability. The three-stage encapsulation process improves the stability of sensitive ingredients by 40%, ensuring a sustained-release period of ≥8 hours.

[0016] The invention can be used in the preparation of liquid beverages, and can also be used in the preparation of powdered granules and far-infrared bagged powdered solid beverages, which greatly increases the application scenarios and is convenient for users to carry and use. DETAILED DESCRIPTION

[0017] Example 1: The formula was adjusted to (mass percentage): diglyceride emulsion 3.2%, L-carnitine tartrate 0.8%, resistant dextrin 8.0%, oat β-glucan 4.0%, konjac glucomannan 2.5%, green tea EGCG nanoliposomes 1.5%, white kidney bean α-AI extract 0.5%, γ-aminobutyric acid microcapsules 0.3%, isomaltooligosaccharide 5.0% The preparation process is as follows: diglyceride emulsion: double-layer phospholipid coating (gellan gum / gum arabic 3:1), pH-responsive release (5.5); L-carnitine sustained-release microspheres: 200nm particle size, entrapment rate 88%; far-infrared activation: 8-14μm wavelength irradiation for 30min.

[0018] Test results: fat oxidation rate: 28.7±3.1 mg / h; visceral fat reduction rate (6 weeks): 34.2±4.7%; duration of satiety: 6.8±1.1 h.

[0019] Example 2: Formula (mass percentage): resistant dextrin 12%, oat β-glucan 5%, konjac glucomannan 3.5%; other ingredients are the same as in Example 1.

[0020] Test results: Intestinal residence time: extended to 12.5±1.8 h; postprandial blood glucose response (AUC): 1234±156mmol·min / L; constipation improvement rate: 92% (n=60).

[0021] Example 3: The formula was adjusted to: 0.5% γ-aminobutyric acid microcapsules and 7% isomaltooligosaccharide; other ingredients were the same as in Example 1. Test results: AMPK pathway activation rate: increased by 3.2 times; ACC key enzyme inhibition rate: 78±6%; continuous drinking for 8 weeks: body fat percentage decreased by 5.7±1.2%.

[0022] Example 4: The preparation process was adjusted as follows: the nanoliposome particle size was optimized to 75 nm; the purity of the white kidney bean extract was ≥98% (α-AI activity 2200 GU / g); and the rest was the same as in Example 1. Test results: visceral fat targeting rate: 89±5%; α-amylase inhibition rate: 72±4%; triglyceride reduction rate: 41±5%.

[0023] Example 5: The thickness of the outer pectin coating was increased to 85 μm, and the porosity of the sustained-release layer was 58%; other conditions were the same as in Example 1.

[0024] Test results: Menthol sustained release time: 12±1.5 h; volatile sulfide inhibition rate: 74±3%; oral odor improvement rate: 89% (n=30).

[0025] Example 6: Process parameters: homogenization temperature 45°C, spray drying air inlet temperature 185°C; other parameters are the same as in Example 1.

[0026] Test results: EGCG retention rate: 93.5±2.1%; degradation rate of heat-sensitive components: <3%; product shelf life: 24 months (25°C).

[0027] Example 7: The pore size distribution of the porous carrier is as follows: 1-5 μm micropores account for 68%; the loading rate is 120±8% (relative to the theoretical value); other aspects are the same as those of Example 1.

[0028] Test results: Solubility of hydrophobic components: 95±3%; Release rate in simulated intestinal fluid (4h): 89±4%; Carrier biocompatibility: Cytotoxicity level 0.

[0029] Comparative Examples 1-3 Comparative Example 1: Triglyceride replaces diglyceride, and there is no far-infrared activation process.

[0030] Test results: Fat oxidation rate: 12.5±1.8 mg / h (p<0.01); visceral fat reduction rate (8 weeks): 18.6±2.4%; increased inflammatory factor IL-6: 23±4 pg / mL.

[0031] Comparative Example 2: Contains resistant dextrin only (8%); no beta-glucan / konjac synergist.

[0032] Test results: duration of satiety: 3.2±0.6 h; blood glucose response curve (AUC): 1892±214 mmol·min / L; intestinal gas production: 2.1±0.3 L / 24h.

[0033] Comparative Example 3: Process differences: Nanoliposomes are not embedded, and white kidney bean extract is directly added.

[0034] Test results: α-amylase inhibition rate: 45±3%; visceral fat targeting rate: 32±6%; gastric discomfort incidence: 18% (n=40).

[0035] Comparative analysis of the effects of the embodiments and comparative examples: Comparison of fat oxidation efficiency: Example 1: Under the synergistic effect of diglyceride emulsion (3.2%) + L-carnitine tartrate (0.8%), the fat oxidation rate reached 28.7±3.1 mg / h (p<0.01).

[0036] Technical mechanism: Double-layer phospholipid coating achieves colon-targeted release, and L-carnitine sustained-release microspheres improve mitochondrial transport efficiency.

[0037] Comparative Example 1 (traditional triglycerides): The fat oxidation rate was only 12.5±1.8 mg / h, and the inflammatory factor IL-6 increased to 23±4 pg / mL.

[0038] Comparative Example 1 has the following defects: triglycerides cannot activate the fat decomposition pathway and easily cause metabolic burden.

[0039] Comparative Example 3 (non-targeted release): The fat oxidation rate was 21.4±2.8 mg / h, but the incidence of gastric discomfort was 18% (n=40).

[0040] Comparative Example 3 has the following defects: direct addition of white kidney bean extract leads to gastric acid damage and low utilization rate of active ingredients.

[0041] Comparison of visceral fat reduction rate: Example 1: Visceral fat area was reduced by 34.2±4.7% in 6 weeks, which was significantly better than the traditional regimen.

[0042] Technical mechanism: Green tea EGCG nanoliposomes (1.5%) + white kidney bean α-AI (0.5%) form a visceral fat-specific targeted decomposition network.

[0043] Comparative Example 1: The visceral fat reduction rate within 8 weeks was only 18.6±2.4%, accompanied by blood lipid rebound.

[0044] Comparative Example 1 has the following defects: lack of metabolic coordination and low fat decomposition efficiency.

[0045] Comparative Example 2 (single fiber formula): The visceral fat reduction rate was 22.5±3.1% within 6 weeks, but the intestinal gas production was as high as 2.1±0.3 L / 24h.

[0046] Comparative Example 2 defect: the single use of resistant dextrin causes intestinal flora imbalance.

[0047] Satiety and blood sugar regulation: Example 1: The duration of satiety was 6.8±1.1 hours (commercially available products ≤4 hours); postprandial blood glucose AUC was 1234±156 mmol·min / L (a decrease of 35%).

[0048] Technical mechanism: The resistant dextrin-oat β-glucan composite fiber network delays gastric emptying, and konjac glucomannan (viscosity ≥20,000 mPa·s) inhibits glucose absorption.

[0049] Comparative Example 2: Postprandial blood glucose AUC1892±214 mmol·min / L, and satiety lasted only 3.2±0.6 hours.

[0050] Defects of Comparative Example 2: A single fiber cannot form a long-lasting barrier and can easily cause blood sugar fluctuations.

[0051] In summary, the present invention achieves a 64% reduction in inflammatory factors and a 124% increase in the abundance of probiotics through far-infrared activation of the bottle (emissivity ≥ 0.92) and the composite fiber matrix; Comparative Example 1 relies on triglycerides, resulting in increased intestinal permeability (FITC-dextran permeability ↑52%); Comparative Example 3 has no sustained-release process, and the active ingredients are rapidly released in the stomach, causing discomfort; the nanoliposomes (50-100nm) of the embodiment of the present invention enable the targeted release rate of EGCG in the intestine to reach 89±5%; three-stage gradient crushing (200-300 mesh) increases the solubility of ginsenoside Rg3 by 37.2%; low-temperature spray drying (inlet air temperature 185-195°C) enables the EGCG retention rate to be ≥93.5%.

[0052] Through the three-dimensional system of "fiber matrix + targeted enzymatic hydrolysis + intelligent release", the present invention can achieve a 129% increase in fat oxidation rate (p<0.01); an 83% increase in visceral fat reduction rate compared to traditional solutions; a 112% extension of the duration of satiety; and a 4-fold increase in metabolic safety (inflammatory factors reduced by 64%).

[0053] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as within the scope of protection of the present invention.

Claims

1. A dietary fiber anti-obesity and fat-reducing composition, characterized in that: Calculated by mass percentage, it contains: Metabolic regulation formula, including mulberry leaf extract 0.8-1.2%, potato extract 0.3-0.5%, γ-aminobutyric acid microcapsules 0.2-0.4%, and isomaltooligosaccharide 4.0-6.0%; Moisture management formula, including 0.5-1.0% prickly pear cactus powder and 0.2-0.4% gardenia powder; Auxiliary regulating formula, including fructooligosaccharide 2.0-4.0%, sucralose 0.05-0.15%, xanthan gum 0.10-0.30%, sodium citrate 0.05-0.15%; Nutritional fortification formula, including taurine 0.1-0.3%, compound nutritional fortifier 0.5-1.0%, and nutritional fortifier containing vitamin B6 / B12, magnesium, iron and zinc; Dietary fiber matrix formula, including resistant dextrin 6.5-8.5%, oat beta-glucan 3.5-4.5%, konjac glucomannan 2.0-3.0%; Fat-targeted decomposition formula, including 3.0-3.5% diglyceride emulsion and 0.5-0.8% L-carnitine tartrate; Visceral fat suppression formula, including green tea EGCG nanoliposomes 1.2-1.8% and white kidney bean α-AI extract 0.4-0.6%.

2. A dietary fiber anti-obesity and lactone-reducing composition according to claim 1, characterized in that: The diglyceride emulsion adopts a double-layer phospholipid coating structure, the inner layer is diglyceride oil with a purity of ≥95%, and the outer layer is a composite wall material of gellan gum or gum arabic, and triggers colon-targeted release when the pH is ≤5.5; The L-carnitine tartrate is loaded via a sustained-release microsphere carrier, the microsphere particle size is 200-500 nm, and the embedding rate is ≥85%.

3. A dietary fiber anti-obesity and lactone-reducing composition according to claim 2, characterized in that: The resistant dextrin is an enzymatically modified product with an average degree of polymerization (DP) of 12 to 18 and a water holding capacity of ≥8 g / g; The oat beta-glucan has a molecular weight of 800,000 to 1.2 million Daltons and a water solubility of ≥98%.

4. A dietary fiber anti-obesity and anti-lactone composition according to claim 3, characterized in that: The green tea EGCG nanoliposomes have a particle size of 50-100 nm and an encapsulation efficiency of ≥90%, and are prepared by an ethanol injection method; The white kidney bean α-AI extract is extracted and purified by gradient cooling, the gradient cooling range is -10°C→25°C, and the α-amylase inhibition activity unit is ≥2000GU / g.

5. A dietary fiber anti-obesity and lactone-reducing composition according to claim 4, characterized in that: The konjac glucomannan is a high-viscosity improved type with a viscosity of ≥20,000 mPa·s (2% aqueous solution); The γ-aminobutyric acid microcapsules use a composite wall material, which is gelatin or gum arabic, and the sustained-release period is ≥8h.

6. A method for preparing the dietary fiber anti-obesity and anti-lactone composition according to any one of claims 1 to 5, characterized in that: include: S1: Diglyceride oil and gellan gum / gum arabic were mixed in a mass ratio of 3:1 and subjected to high-pressure homogenization to prepare double-layer microcapsules. The high-pressure homogenization pressure was 120 MPa and the number of cycles was 3; S2: Disperse L-carnitine tartrate in maltodextrin solution and spray dry to prepare sustained-release microspheres; S3: Resistant dextrin, oat β-glucan and inulin are mixed in proportion and dispersed with the aid of ultrasound to form a fiber network, wherein the ultrasound frequency of the ultrasound-assisted dispersion is 40 kHz and the power is 200 W; S4: Mulberry leaf extract, potato extract, green tea EGCG and phosphatidylcholine were mixed in a mass ratio of 2:1:1:4 and nanoliposomes were prepared by ethanol injection method; S5: mixing the white kidney bean extract and the konjac glucomannan solution in a volume ratio of 3:1, and filtering and purifying the mixture through a ceramic membrane, wherein the pore size of the ceramic membrane is 0.2 μm; S6: After mixing the above components with taurine, compound nutritional enhancers and other auxiliary materials in proportion, spray drying or freeze drying is carried out to prepare a composition.

7. The method for preparing the dietary fiber anti-obesity and lactone-reducing composition according to claim 6, characterized in that: The high-pressure homogenization temperature in step S1 is controlled at 45-55° C. to avoid oxidation of diglycerides.

8. The method for preparing the dietary fiber anti-obesity and lactone-reducing composition according to claim 6, characterized in that: The ethanol concentration in step S4 is 75% (v / v), and the injection speed is 5 mL / min.

9. An application of the dietary fiber anti-obesity and anti-lactone composition according to any one of claims 1 to 5, characterized in that: It is applicable to any one or more of powdered granules, liquid beverages, and far-infrared bagged powdered solid beverages.