Anti-fatigue essential oil composition and preparation method thereof

Abstract

The present invention provides an anti-fatigue essential oil composition and a preparation method thereof, belonging to the technical field of compound essential oils. The essential oil composition comprises a compound essential oil and a eutectic composition; the compound essential oil comprises 1 to 25 parts of Torreya grandis aril essential oil, 1 to 20 parts of cinnamon leaf essential oil, 1 to 20 parts of lemon essential oil, and 1 to 15 parts of ginger essential oil; the eutectic composition comprises 1 to 3 parts of borneol and 1 to 10.5 parts of menthol. A potassium citrate solution is added to Torreya grandis arils, a sodium acetate solution is added to cinnamon leaves, a potassium citrate solution is added to fresh lemons, and purified water is added to ginger. The juices are squeezed separately and then distilled to obtain Torreya grandis aril essential oil, cinnamon leaf essential oil, lemon essential oil, and ginger essential oil. The present invention combines the four essential oils in a proportioned mixture to produce a compound essential oil, and adds the eutectic composition to produce an essential oil composition. The essential oil composition has significant fatigue-relieving and mentally refreshing effects, and is not easily tolerated.

Description

Technical Field

[0001] The invention belongs to the technical field of compound essential oils, and in particular relates to an anti-fatigue essential oil composition and a preparation method thereof. Background Art

[0002] Aromatherapy is a traditional natural therapy that involves formulating aromatic drugs into suitable dosage forms and applying them locally or systemically through massage, topical application, moxibustion, fumigation, or internal administration to prevent and treat diseases. It boasts advantages such as ease of use, high safety, and low cost. Essential oils are widely used as aromatherapy drugs. Their inherent volatility, strong permeability, and high bioactivity enable them to achieve significant effects, including rapid efficacy and high bioavailability. They are widely used clinically, particularly for refreshing the mind and combating fatigue.

[0003] Currently, aromatherapy for fatigue is primarily based on highly fragrant single essential oils such as mint, rosemary, and lemon. However, these oils are generally ineffective, slow to take effect, fail to fully regulate bodily functions, and offer incomplete treatment. They can also lead to resistance to the single essential oils. Compound essential oils are made by blending two or more single essential oils in appropriate proportions. The synergistic effects of the individual essential oils allow for greater therapeutic efficacy using a smaller amount.

[0004] However, existing compound essential oils have drawbacks in terms of anti-fatigue efficacy, such as uncertain efficacy and slow onset of effect. Furthermore, existing plant essential oils are produced through methods such as pressing, ultrasonic immersion, microwave immersion, and steam distillation, which have drawbacks such as low extraction rates, easy loss of active ingredients, and susceptibility to deterioration. Summary of the Invention

[0005] In view of this, the present invention aims to provide an anti-fatigue essential oil composition and a preparation method thereof, aiming to solve at least one technical problem among the background technology.

[0006] The present invention is achieved in that:

[0007] A first aspect of the present invention provides an anti-fatigue essential oil composition, the essential oil composition comprising a compound essential oil and a eutectic composition;

[0008] The compound essential oil comprises the following essential oil components in parts by weight:

[0009] 1 to 25 parts of Torreya grandis aril essential oil;

[0010] 1 to 20 parts of cinnamon leaf essential oil;

[0011] 1 to 20 parts of lemon essential oil;

[0012] 1 to 15 parts of ginger essential oil;

[0013] The eutectic composition comprises the following raw materials in parts by weight:

[0014] 1 to 3 parts borneol;

[0015] 1 part to 10.5 parts of menthol;

[0016] The method comprises adding a potassium citrate solution to the torreya aril, squeezing the torreya aril into juice, and then distilling the torreya aril essential oil;

[0017] Adding sodium acetate solution to cinnamon leaves, squeezing into juice, and distilling to obtain the cinnamon leaf essential oil;

[0018] Adding potassium citrate solution to fresh lemons, squeezing the juice, and then distilling to obtain the lemon essential oil;

[0019] Purified water is added to ginger, the ginger is squeezed into juice, and then the ginger essential oil is obtained by distillation;

[0020] The compound essential oil is evenly mixed with the melted eutectic composition to obtain the essential oil composition.

[0021] Preferably, the compound essential oil comprises the following essential oil components in parts by weight:

[0022] 20-25 parts of Torreya grandis aril essential oil;

[0023] 15-20 parts cinnamon leaf essential oil;

[0024] 15-20 parts of lemon essential oil;

[0025] 10-15 parts of ginger essential oil;

[0026] The eutectic composition comprises the following raw materials in parts by weight:

[0027] 2~3 parts borneol;

[0028] 7 to 10.5 parts of menthol.

[0029] Preferably, in the compound essential oil, according to the weight ratio, Torreya grandis aril essential oil: cinnamon leaf essential oil: lemon essential oil: ginger essential oil = (4-5): (3-4): (3-4): 3;

[0030] In the eutectic composition, the weight ratio of borneol to menthol is 1:3-4.

[0031] Preferably, in the essential oil composition, the mass ratio of the eutectic composition is 10% to 15%.

[0032] Preferably, in the compound essential oil, according to the weight ratio, Torreya grandis aril essential oil: cinnamon leaf essential oil: lemon essential oil: ginger essential oil = 5:4:4:3;

[0033] In the eutectic composition, the weight ratio of borneol to menthol is 1:3.5.

[0034] A second aspect of the present invention provides a method for preparing the above-mentioned anti-fatigue essential oil composition, which comprises the following steps:

[0035] The cleaned Torreya grandis aril, cinnamon leaves, fresh lemon and ginger are granulated or sliced ​​respectively;

[0036] The treated Torreya grandis aril is squeezed, and potassium citrate solution is added during the squeezing process to obtain Torreya grandis aril juice;

[0037] The processed cinnamon leaves are squeezed, and sodium acetate solution is added during the squeezing process to obtain cinnamon leaf juice;

[0038] The processed fresh lemons are squeezed, and potassium citrate solution is added during the squeezing process to obtain lemon juice;

[0039] The processed ginger is squeezed, and purified water is added during the squeezing process to obtain ginger juice;

[0040] Torreya grandis aril juice, cinnamon leaf juice, lemon juice, and ginger juice are respectively added to corresponding steam distillation devices and heated, and fractions are taken out and dried to obtain Torreya grandis aril essential oil, cinnamon leaf essential oil, lemon essential oil, and ginger essential oil, respectively;

[0041] Torreya grandis aril essential oil, cinnamon leaf essential oil, lemon essential oil and ginger essential oil are mixed in proportion to obtain a compound essential oil;

[0042] The compound essential oil is evenly mixed with the melted eutectic composition to obtain the essential oil composition.

[0043] Preferably, the compound essential oil is uniformly mixed with the melted eutectic composition to obtain the essential oil composition, which is specifically as follows:

[0044] Mixing borneol and menthol in a container, heating to ≤50°C to completely melt them, and stirring for more than 20 minutes to obtain a eutectic composition;

[0045] Torreya grandis aril essential oil, cinnamon leaf essential oil, lemon essential oil and ginger essential oil are added to the eutectic composition, and the mixture is stirred and mixed evenly.

[0046] Preferably, when preparing the Torreya grandis aril juice, the ratio of Torreya grandis aril to potassium citrate solution is 10:6-8 by weight, and the pH of the potassium citrate solution is 7.5-8.5;

[0047] When preparing the cinnamon leaf juice, the ratio of cinnamon leaf to sodium acetate solution is 10:10-12, and the pH value of the sodium acetate solution is 7.5-8.5;

[0048] When preparing the lemon juice, the ratio of fresh lemon to potassium citrate solution is 10:4-6, and the pH value of the potassium citrate solution is 8-9;

[0049] When preparing the ginger juice, the ratio of ginger to purified water is 10:4-6.

[0050] Preferably, when preparing the Torreya grandis aril essential oil, the heating temperature in the steam distillation apparatus is 90° C. to 100° C., the pressure is 300 mbar to 500 mbar, and the heating time is 2 h to 4 h;

[0051] When preparing the cinnamon leaf essential oil, the heating temperature in the steam distillation apparatus is 70° C. to 100° C., the pressure is 300 mbar to 500 mbar, and the heating time is 3 h to 5 h;

[0052] When preparing the lemon essential oil, the heating temperature in the steam distillation apparatus is 90° C. to 100° C., the pressure is 300 mbar to 500 mbar, and the heating time is 2 h to 4 h;

[0053] When preparing the ginger essential oil, the heating temperature in the steam distillation device is 80° C. to 100° C., the pressure is 300 mbar to 500 mbar, and the heating time is 2 h to 4 h.

[0054] Torreya grandis aril essential oil: A volatile aromatic substance extracted from the aril of Torreya grandis of the Taxaceae family. It has antibacterial and anti-inflammatory effects, promotes skin repair, and relieves muscle pain. Its active ingredients such as phenols and terpenes give it antioxidant, moisturizing and skin barrier regulating effects. It can also be used for insect repellent, mood improvement and other scenarios.

[0055] Cinnamon leaf essential oil: An aromatic oil distilled from cinnamon leaves, its primary active ingredient is cinnamaldehyde. Its core benefits include antibacterial, anti-inflammatory, and blood circulation promoting properties. It's commonly used for relieving muscle pain and skincare.

[0056] Lemon essential oil: A volatile substance extracted from lemons, it has the function of purifying the air, sterilizing and disinfecting, improving the indoor environment, and can also fade acne scars, shrink pores, and has multiple functions such as antibacterial, enhancing immunity, improving mood, and skin care.

[0057] Ginger essential oil: A natural essential oil extracted from ginger that can promote blood circulation, improve cold hands and feet, relieve colds, and play a role in dispelling colds. It also has the effects of aiding digestion, enhancing immunity, and relieving fatigue.

[0058] Compared to existing technologies, the present invention's compound essential oil, prepared by blending Torreya grandis aril essential oil, cinnamon leaf essential oil, lemon essential oil, and ginger essential oil in appropriate proportions, exhibits significant fatigue-relieving and mentally refreshing effects, while being less easily tolerated. Blending the compound essential oil with a eutectic composition slows the release rate of the essential oil's active ingredients, preventing irritation caused by sudden local concentration increases while maintaining a long-lasting effect.

[0059] The present invention adds a small amount of specific solvent during the process of squeezing and preparing the single essential oil, and its beneficial effects include:

[0060] 1. Add potassium citrate solution (pH=7.5~8.5) when pressing the Torreya grandis aril, which will neutralize the acidic substances in the Torreya grandis aril, slow down the decay and oxidation rate of the Torreya grandis aril, reduce the corrupt odor in the essential oil, and make the essential oil dissolve better.

[0061] 2. When cinnamon leaves are squeezed, sodium acetate solution (pH=7.5~8.5) neutralizes the acidic substances in the cinnamon leaves, slows down their decay and oxidation rate, reduces the corrupt smell in the essential oil, and makes the essential oil dissolve better.

[0062] 3. Potassium citrate solution (pH=8~9) is added during the squeezing process of fresh lemons, which neutralizes the citric acid and other acidic substances in the raw materials, slows down their decay and oxidation rate, and increases the content of limonene in the essential oil.

[0063] 4. Adding purified water during the ginger squeezing process increases the dissolution of essential oils.

[0064] The present invention adopts a pressing + steam distillation method when preparing essential oils. The pressing can be carried out at the place of production, which can solve the problems of oxidation, corruption, collision and seepage of raw materials during processing and transportation, improve the quality of single essential oils, and increase the output of essential oils; since only juice is transported, transportation costs are reduced; the juice can be stacked in ton barrels, reducing storage costs; only juice is extracted, which improves extraction efficiency and reduces extraction energy consumption. BRIEF DESCRIPTION OF THE DRAWINGS

[0065] Figure 1 This is a GC-MS total ion current chromatogram of the Torreya grandis aril essential oil prepared in Example 1 of the present invention;

[0066] Figure 2 This is a GC-MS total ion current chromatogram of the cinnamon leaf essential oil prepared in Example 1 of the present invention;

[0067] Figure 3 This is a GC-MS total ion current chromatogram of the lemon essential oil prepared in Example 1 of the present invention;

[0068] Figure 4 GC-MS total ion current chromatogram of ginger essential oil prepared in Example 1 of the present invention;

[0069] Figure 5 This is a GC-MS total ion current chromatogram of the Torreya grandis aril essential oil prepared in Comparative Example 1 of the present invention;

[0070] Figure 6 This is a GC-MS total ion current chromatogram of the cinnamon leaf essential oil prepared in Comparative Example 2 of the present invention;

[0071] Figure 7 This is a GC-MS total ion current chromatogram of the lemon essential oil prepared in Comparative Example 3 of the present invention;

[0072] Figure 8 This is a GC-MS total ion current chromatogram of the ginger essential oil prepared in Comparative Example 4 of the present invention;

[0073] Figure 9 This is a comparison chart of the total movement distance of mice in the open field test of Example 5 of the present invention, the control group A, the positive drug group B, the aerosol test groups C1 to C4, and the non-aerosol test group D;

[0074] Figure 10 This is a comparison chart of the average speeds of mice in the control group A, the positive drug group B, the aerosol test groups C1 to C4, and the non-aerosol test group D in the open field test of Example 5 of the present invention;

[0075] Figure 11 This is a comparison chart of the rest time of mice in the open field test of Example 5 of the present invention, the control group A, the positive drug group B, the aerosol test groups C1 to C4, and the non-aerosol test group D;

[0076] Figure 12 This is a comparison chart of the total movement distance of mice in the open field test of Example 5 of the present invention, the control group A, the positive drug group B, the nebulization test group C1, and the nebulization comparison groups E1 to E6;

[0077] Figure 13 This is a comparison chart of the average speeds of mice in the open field test of Example 5 of the present invention, the control group A, the positive drug group B, the aerosol test group C1, and the aerosol comparison groups E1 to E6;

[0078] Figure 14 This is a comparison chart of the rest time of mice in the open field test of Example 5 of the present invention, the control group A, the positive drug group B, the nebulization test group C1, and the nebulization comparison groups E1 to E6;

[0079] Figure 15 This is a comparison chart of the sleep latency of mice in the control group A, the positive drug group B, the nebulized test group C1, and the non-nebulized test group D in the amobarbital sodium suprathreshold sleep induction test of Example 5 of the present invention;

[0080] Figure 16 This is a comparison chart of the sleep time of mice in the control group A, the positive drug group B, the nebulized test group C1, and the non-nebulized test group D in the amobarbital sodium suprathreshold sleep induction test of Example 5 of the present invention;

[0081] Figure 17 This is a comparison chart of the sleep latency of mice in the control group A, the positive drug group B, the nebulization test group C1, and the nebulization comparison groups E1 to E6 in the suprathreshold sleep induction test of amobarbital sodium in Example 5 of the present invention;

[0082] Figure 18 This is a comparison chart of the sleep time of mice in the control group A, the positive drug group B, the nebulization test group C1, and the nebulization comparison groups E1 to E6 in the amobarbital sodium suprathreshold sleep induction test of Example 5 of the present invention;

[0083] Figure 19 This is a comparison chart of the swimming rest time of mice in the control group A, the positive drug group B, the aerosol test group C1, and the non-aerosol test group D in the forced swimming test of Example 5 of the present invention;

[0084] Figure 20 This is a comparison chart of the swimming rest time of mice in the control group A, the positive drug group B, the nebulization test group C1, and the nebulization comparison groups E1 to E6 in the forced swimming test of Example 5 of the present invention;

[0085] Figure 21 This is a comparison chart of the total movement distance of mice in the control group A, the positive drug group B, the aerosol test group C1, and the non-aerosol test group D in the multiple-dose open field test of Example 5 of the present invention;

[0086] Figure 22 This is a comparison chart of the average speeds of mice in the control group A, the positive drug group B, the aerosol test group C1, and the non-aerosol test group D in the multiple-dose open field test of Example 5 of the present invention;

[0087] Figure 23 This is a comparison chart of the rest time of mice in the control group A, the positive drug group B, the aerosol test group C1, and the non-aerosol test group D in the multiple-dose open field test of Example 5 of the present invention;

[0088] Figure 24 This is a comparison chart of the average speeds of mice in the control group A, the positive drug group B, the aerosol test group C1, and the aerosol comparison groups E1 to E6 in the multiple-dose open field test of Example 5 of the present invention;

[0089] Figure 25This is a comparison chart of the total movement distance of mice in the control group A, the positive drug group B, the nebulization test group C1, and the nebulization comparison groups E1 to E6 in the multiple-dose open field test of Example 5 of the present invention;

[0090] Figure 26 This is a comparison chart of the rest time of mice in the control group A, the positive drug group B, the nebulization test group C1, and the nebulization comparison groups E1 to E6 in the multiple-dose open field test of Example 5 of the present invention. DETAILED DESCRIPTION

[0091] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the embodiments. It should be understood that the specific implementation cases described herein are only used to explain the present invention and are not intended to limit the present invention.

[0092] The invention provides an anti-fatigue essential oil composition, which comprises compound essential oil and a eutectic composition.

[0093] Specifically, the compound essential oil contains the following essential oil components by weight: 1-25 parts of Torreya grandis aril essential oil; 1-20 parts of cinnamon leaf essential oil; 1-20 parts of lemon essential oil; and 1-15 parts of ginger essential oil. Preferably, the essential oil comprises 20-25 parts of Torreya grandis aril essential oil, 15-20 parts of cinnamon leaf essential oil, 15-20 parts of lemon essential oil, and 10-15 parts of ginger essential oil. In the compound essential oil, the weight ratio of Torreya grandis aril essential oil: cinnamon leaf essential oil: lemon essential oil: ginger essential oil is (4-5): (3-4): (3-4): 3, preferably 5:4:4:3.

[0094] Specifically, the eutectic composition comprises the following raw materials in parts by weight: 1 to 3 parts borneol; 1 to 10.5 parts menthol; preferably, 2 to 3 parts borneol; 7 to 10.5 parts menthol. The weight ratio of borneol to menthol is 1:3 to 4, preferably 1:3.5.

[0095] Specifically, in the essential oil composition, the mass proportion of the eutectic composition is 10% to 15%.

[0096] A potassium citrate solution is added to Torreya grandis aril, a sodium acetate solution is added to cinnamon leaves, a potassium citrate solution is added to fresh lemons, and purified water is added to ginger; the products are squeezed into juice respectively, and then distilled to obtain the Torreya grandis aril essential oil, cinnamon leaf essential oil, lemon essential oil, and ginger essential oil.

[0097] Borneol and menthol are mixed in a container, heated to ≤50° C. to completely melt, and stirred for more than 20 minutes to obtain a eutectic composition.

[0098] The compound essential oil is evenly mixed with the melted eutectic composition to obtain the essential oil composition.

[0099] Example 1

[0100] An anti-fatigue essential oil composition comprises compound essential oil and a eutectic composition.

[0101] Calculated by weight, the compound essential oil includes: 25 parts of Torreya grandis aril essential oil; 20 parts of cinnamon leaf essential oil; 20 parts of lemon essential oil; and 15 parts of ginger essential oil.

[0102] In parts by weight, the eutectic composition includes: 3 parts of borneol and 10.5 parts of menthol.

[0103] 1. The production method of Torreya grandis aril essential oil is as follows:

[0104] (1) Wash the fresh Torreya grandis aril and crush it into coarse particles;

[0105] (2) Add 70 g of potassium citrate solution with a pH of 8 to 100 g of coarse Torreya grandis aril particles and squeeze to obtain Torreya grandis aril juice;

[0106] (3) The Torreya grandis aril juice was added to a steam distillation apparatus and heated at a temperature of 90°C to 100°C, a pressure of 400 mbar, and a heating time of 3 h. The fraction was taken out and dried to obtain Torreya grandis aril essential oil.

[0107] The essential oil of Torreya grandis aril was injected into GC-MS for analysis and detection, and the total ion current chromatogram was as follows: Figure 1 shown.

[0108] 2. The production method of cinnamon leaf essential oil is as follows:

[0109] (1) Wash the cinnamon leaves and crush them into coarse particles;

[0110] (2) Add 110 g of sodium acetate solution with a pH of 8 to 100 g of cinnamon leaf coarse particles and squeeze to obtain cinnamon leaf juice;

[0111] (3) The cinnamon leaf juice was added to a steam distillation apparatus and heated at a temperature of 70°C to 100°C, a pressure of 400 mbar, and a heating time of 4 h. The fraction was taken out and dried to obtain cinnamon leaf essential oil.

[0112] The prepared cinnamon leaf essential oil was injected into GC-MS for analysis and detection, and its total ion current chromatogram was as follows: Figure 2 shown.

[0113] 3. The production method of lemon essential oil is as follows:

[0114] (1) Wash the fresh lemon and cut it into small slices;

[0115] (2) Add 50 g of potassium citrate solution with pH = 9 to 100 g of fresh lemon slices and squeeze to obtain lemon juice;

[0116] (3) The lemon juice is added to a steam distillation apparatus and heated at a temperature of 90°C to 100°C, a pressure of 400 mbar, and a heating time of 3 h. The distillate is taken out and dried to obtain lemon essential oil.

[0117] The prepared lemon essential oil was injected into GC-MS for analysis and detection, and its total ion current chromatogram was as follows: Figure 3 shown.

[0118] 4. The production method of ginger essential oil is as follows:

[0119] (1) Wash the ginger and cut into small pieces;

[0120] (2) Add 50 g of purified water to 100 g of ginger slices and squeeze to obtain ginger juice;

[0121] (3) The ginger juice was added to a steam distillation apparatus and heated at a temperature of 80°C to 100°C, a pressure of 400 mbar, and a heating time of 3 h. The distillate was taken out and dried to obtain ginger essential oil.

[0122] The prepared ginger essential oil was injected into GC-MS for analysis and detection, and its total ion current chromatogram was as follows: Figure 4 shown.

[0123] 5. A method for preparing a eutectic composition, comprising the following steps:

[0124] Borneol and menthol were mixed in a container, heated to about 45° C. to completely melt, and stirred for more than 20 minutes to obtain a eutectic composition.

[0125] 6. A method for preparing an anti-fatigue essential oil composition, comprising the following steps:

[0126] According to the proportion of the components, Torreya grandis aril essential oil, cinnamon leaf essential oil, lemon essential oil and ginger essential oil are added to the eutectic composition, and the mixture is stirred and evenly mixed to obtain the product.

[0127] The anti-fatigue essential oil composition is an inhalation dosage form for administration by sniffing.

[0128] Example 2

[0129] An anti-fatigue essential oil composition comprises a compound essential oil and a eutectic composition. The preparation method thereof is the same as that of Example 1, the difference being only in the components in the compound essential oil and the eutectic composition.

[0130] Calculated by weight, the compound essential oil includes: 20 parts of Torreya grandis aril essential oil; 20 parts of cinnamon leaf essential oil; 20 parts of lemon essential oil; and 15 parts of ginger essential oil.

[0131] In parts by weight, the eutectic composition includes: 2.5 parts of borneol and 8.8 parts of menthol.

[0132] Example 3

[0133] An anti-fatigue essential oil composition comprises a compound essential oil and a eutectic composition. The preparation method thereof is the same as that of Example 1, the difference being only in the components in the compound essential oil and the eutectic composition.

[0134] Calculated by weight, the compound essential oil includes: 20 parts of Torreya grandis aril essential oil; 15 parts of cinnamon leaf essential oil; 20 parts of lemon essential oil; and 15 parts of ginger essential oil.

[0135] Calculated by weight, the eutectic composition includes: 2 parts of borneol and 7 parts of menthol.

[0136] Example 4

[0137] An anti-fatigue essential oil composition comprises a compound essential oil and a eutectic composition. The preparation method thereof is the same as that of Example 1, the difference being only in the components in the compound essential oil and the eutectic composition.

[0138] Calculated by weight, the compound essential oil includes: 20 parts of Torreya grandis aril essential oil; 20 parts of cinnamon leaf essential oil; 15 parts of lemon essential oil; and 15 parts of ginger essential oil.

[0139] Calculated by weight, the eutectic composition includes: 2 parts of borneol and 7 parts of menthol.

[0140] Comparative Example 1

[0141] Compared with Example 1, when preparing the Torreya grandis aril essential oil in this comparative example, no potassium citrate solution was added in the pressing step.

[0142] The production method of Torreya grandis aril essential oil is as follows:

[0143] (1) Wash the fresh Torreya grandis aril and crush it into coarse particles;

[0144] (2) 100 g of coarse Torreya grandis aril particles were squeezed to obtain Torreya grandis aril juice;

[0145] (3) The Torreya grandis aril juice was added to a steam distillation apparatus and heated at a temperature of 90°C to 100°C, a pressure of 400 mbar, and a heating time of 3 h. The fraction was taken out and dried to obtain Torreya grandis aril essential oil.

[0146] The essential oil of Torreya grandis aril prepared in this comparative example was injected into GC-MS for analysis and detection, and the total ion current chromatogram thereof was as follows: Figure 5 shown.

[0147] Depend on Figure 1and Figure 5 Comparison shows that the content of essential oil components of Torreya grandis aril in Example 1 is significantly higher than that of essential oil components of Torreya grandis aril in Comparative Example 1, indicating that the addition of potassium citrate solution during squeezing helps to dissolve the essential oil components of Torreya grandis aril and improve the quality of essential oil.

[0148] Comparative Example 2

[0149] Compared with Example 1, when preparing cinnamon leaf essential oil in this comparative example, no sodium acetate solution was added in the pressing step.

[0150] The steps for making cinnamon leaf essential oil are as follows:

[0151] (1) Wash the cinnamon leaves and crush them into coarse particles;

[0152] (2) 100 g of cinnamon leaf coarse particles were squeezed to obtain cinnamon leaf juice;

[0153] (3) The cinnamon leaf juice was added to a steam distillation apparatus and heated at a temperature of 70°C to 100°C, a pressure of 400 mbar, and a heating time of 4 h. The fraction was taken out and dried to obtain cinnamon leaf essential oil.

[0154] The cinnamon leaf essential oil prepared in this comparative example was injected into GC-MS for analysis and detection, and its total ion current chromatogram was as follows: Figure 6 shown.

[0155] Depend on Figure 2 and Figure 6 Comparison shows that the content of cinnamon leaf essential oil components in Example 1 is significantly higher than that in Comparative Example 2, indicating that the addition of sodium acetate solution during squeezing helps dissolve the cinnamon leaf essential oil components and improves the quality of the essential oil.

[0156] Comparative Example 3

[0157] Compared with Example 1, when preparing lemon essential oil in this comparative example, potassium citrate solution was not added in the pressing step.

[0158] The steps for making lemon essential oil are as follows:

[0159] (1) Wash the fresh lemon and cut it into small slices;

[0160] (2) 100g of fresh lemon slices were squeezed to obtain lemon juice;

[0161] (3) The lemon juice is added to a steam distillation apparatus and heated at a temperature of 90°C to 100°C, a pressure of 400 mbar, and a heating time of 3 h. The distillate is taken out and dried to obtain lemon essential oil.

[0162] The lemon essential oil prepared in this comparative example was injected into GC-MS for analysis and detection, and its total ion current chromatogram was as follows: Figure 7 shown.

[0163] Depend on Figure 3 and Figure 7 Comparison shows that the content of lemon essential oil components in Example 1 is significantly higher than that in Comparative Example 3, indicating that the addition of potassium citrate solution during squeezing helps dissolve the lemon essential oil components and improves the quality of the essential oil.

[0164] Comparative Example 4

[0165] Compared with Example 1, when preparing ginger essential oil in this comparative example, purified water was not added in the pressing step.

[0166] The production method of ginger essential oil is as follows:

[0167] (1) Wash the ginger and cut into small pieces;

[0168] (2) 100 g of ginger slices were squeezed to obtain ginger juice;

[0169] (3) The ginger juice was added to a steam distillation apparatus and heated at a temperature of 80°C to 100°C, a pressure of 400 mbar, and a heating time of 3 h. The distillate was taken out and dried to obtain ginger essential oil.

[0170] The ginger essential oil prepared in this comparative example was injected into GC-MS for analysis and detection, and its total ion current chromatogram was as follows: Figure 8 shown.

[0171] Depend on Figure 4 and Figure 8 Comparison shows that the content of ginger essential oil components in Example 1 is significantly higher than that in Comparative Example 4, indicating that adding purified water during squeezing helps dissolve the ginger essential oil components and improves the quality of the essential oil.

[0172] The yields of the essential oils obtained in Example 1 and Comparative Examples 1 to 4 were measured, and the results are shown in Table 1.

[0173] In addition, it is difficult to guarantee the freshness of raw materials 100% in actual implementation. For example, Torreya grandis arils and lemons are very easy to oxidize and rot during the harvesting, processing and transportation process, which makes the original Torreya grandis fragrance and lemon fragrance turn into sourness. Cinnamon leaves and ginger are subjected to collision and temperature increase during the harvesting, processing and transportation process, which will produce a large amount of liquid flow, resulting in the loss of effective ingredients, reducing the production of essential oils, and easily breeding mold, affecting the quality of essential oils. If the essential oils are directly squeezed without solvents during preparation, the quality of the essential oils is very likely to deteriorate. In the present invention, potassium citrate and sodium acetate are used as solvents to improve the degree of corruption of essential oils.

[0174] In Example 1 of the present invention and Comparative Examples 1 to 4, approximately 20 wt % of the raw materials were slightly rotten (the rotten area was 10% to 15% of the total area). The taste of the essential oil was tested, and the results are shown in Table 1.

[0175] Table 1

[0176]

[0177] It can be seen from the data in Table 1 that without adding potassium citrate solution (pH=8), the yield of Torreya grandis aril essential oil in Comparative Example 1 is reduced by about 15% compared with that in Example 1; and the Torreya grandis aril essential oil in Comparative Example 1 has a slight sour odor;

[0178] Without adding sodium acetate solution (pH=8), the yield of cinnamon leaf essential oil in Comparative Example 2 was about 12% lower than that in Example 1; and the cinnamon leaf essential oil in Comparative Example 2 had a slightly sour odor;

[0179] Without adding potassium citrate solution (pH=9), the yield of lemon essential oil in Comparative Example 3 was reduced by about 11% compared with that in Example 1; and the lemon aroma of the lemon essential oil in Comparative Example 3 was slightly weaker;

[0180] Without adding purified water, the ginger essential oil yield in Comparative Example 4 was reduced by about 18% compared with that in Example 1.

[0181] Example 5

[0182] 1. Open field test

[0183] Experimental Animals: 78 male Kunming mice of similar health status were randomly divided into 13 groups, with 6 mice in each group. The experimental groups were as follows:

[0184] Control group (Group A): Mice were placed in a nebulizer chamber and atomized 10 mL of distilled water using an aromatherapy machine. The mice in this group inhaled the nebulizer for 15 minutes daily.

[0185] Positive drug group (Group B): mice were gavaged with caffeine daily at a dose of 57 mg / kg body weight;

[0186] Nebulization test groups (Groups C1 to C4): Mice were placed in a nebulization chamber and a nebulizer was used to atomize the essential oil compositions prepared in Examples 1 to 4 (10 mL) for 15 minutes daily. To increase the nebulization efficiency of the essential oils, a small amount of distilled water was used to disperse the essential oils.

[0187] Non-atomization test group (Group D): Mice were placed in an atomization chamber, and 10 mL of the essential oil composition prepared in Example 1 was placed in an open vial and fixed in the atomization chamber. The essential oil composition was allowed to evaporate freely, and the mice in this group inhaled it for 15 minutes daily.

[0188] Nebulization comparison groups (E1-E6): Mice were placed in a nebulization chamber and a nebulizer was used to atomize the essential oil composition (essential oil composition numbered 1-6 in Table 2, with a dosage of 10 mL). Each group of mice inhaled the composition for 15 minutes daily. To increase the efficiency of essential oil atomization, a small amount of distilled water was used to disperse the essential oil.

[0189] Table 2

[0190]

[0191] Experimental process: The drug was administered for 3 consecutive days. Every day, the control group, the nebulized test group, the nebulized comparison group, and the non-nebulized test group inhaled distilled water or essential oil for 15 minutes through nebulization or non-nebulization, and then rested for 15 minutes before the open field test. The positive drug group was gavaged with caffeine for 30 minutes before the open field test. After 3 minutes of familiarization with the environment, behavioral parameters within 5 minutes were recorded, namely the total movement distance (cm) and average speed (cm / s). The results are as follows: Figures 9 to 14 As shown, * indicates P < 0.05, ** indicates P < 0.01, and # indicates P < 0.05.

[0192] like Figure 9 、 Figure 10 、 Figure 11 As shown, after one day of administration, compared with the control group A, the movement distance of mice in the positive drug group B, the nebulized test groups C1-C4, and the non-nebulized test group D all increased significantly (P < 0.01), the average speed increased significantly (P < 0.01), and the rest time was significantly shortened (P < 0.01). This indicates that the essential oil compositions of Examples 1 to 4 of the present invention can significantly improve the autonomous movement ability of normal mice and have an invigorating effect, and the nebulized test effect is superior to the non-nebulized test effect. After two days of administration, the test results were basically consistent with those on the first day. After three days of administration, compared with the control group, the total movement distance of mice in the positive drug group was significantly increased (P < 0.01), while the average speed and rest time did not differ significantly. The movement distance of mice in the nebulized test groups C1-C4 and the non-nebulized test group D all increased significantly (P < 0.01), the average speed increased significantly (P < 0.01), and the rest time was significantly shortened (P < 0.01). The results showed that the positive drug group B developed a certain resistance on the third day and the drug efficacy decreased. Compared with the positive drug group, the essential oil compositions of Examples 1 to 4 can continuously promote the autonomous activities of mice, and the atomization test effect of the essential oil composition of Example 1 is better than that of the non-atomization test effect.

[0193] In the nebulization comparison group, groups E1 to E4 refer to the compound essential oils lacking Torreya grandis aril essential oil, cinnamon leaf essential oil, lemon essential oil or ginger essential oil, respectively; Figure 12 、 Figure 13 、 Figure 14As shown, compared with the control group A, the movement distance and average speed of mice in the nebulized comparison groups E1 to E6 were significantly increased (P<0.05, P<0.01), and the rest time was significantly shortened (P<0.01). Compared with the nebulized test group C1, the movement distance and average speed of mice in groups E1 to E6 were reduced (P<0.05), and the rest time was increased (P<0.05). This indicates that the lack of any essential oil in the essential oil composition and the lack of borneol or menthol in the eutectic composition significantly reduce the promoting effect on the autonomous movement of mice, and that the promoting effect of the essential oil composition of the present invention is the result of the synergistic effect of its components.

[0194] 2. Amobarbital sodium suprathreshold sleep induction test

[0195] The experimental animals and experimental groups were the same as those in the open field test.

[0196] Experimental process: Control group A, nebulized test group C1, nebulized comparison group E1 to E6, and non-nebulized test group D were inhaled distilled water or essential oil for 15 minutes, followed by a 15-minute rest and intraperitoneal injection of 137.5 mg / kg of amobarbital sodium to induce sleep. Positive drug group B was gavaged with caffeine for 30 minutes and then intraperitoneally injected with 137.5 mg / kg of amobarbital sodium to induce sleep. The sleep latency and sleep time of mice in each group were recorded. The results are as follows: Figures 15 to 18 As shown, * indicates P < 0.05, ** indicates P < 0.01, and # indicates P < 0.05.

[0197] like Figure 15 、 Figure 16 As shown, compared with the control group A, the essential oil compositions of the positive drug group B, the nebulization test group C1 and the non-nebulization test group D had no significant effect on the sleep latency of mice (P>0.05), while the sleep time was significantly shortened (P<0.05, P<0.01), indicating that the essential oil composition of Example 1 has a good wake-up effect when inhaled.

[0198] like Figure 17 、 Figure 18 As shown, compared with the atomization test group C1, groups E1 to E6 had no significant effect on the sleep latency of mice (P>0.05), while the sleep time was significantly increased (P<0.05), indicating that the lack of any essential oil in the essential oil composition and the lack of borneol or menthol in the low-melting composition would significantly reduce the wakefulness-promoting effect, and that the promoting effect of the essential oil composition of the present invention is the result of the synergistic effect of each component therein.

[0199] 3. Forced swim test

[0200] The experimental animals and experimental groups were the same as those in the open field test.

[0201] Experimental process: The control group A, the nebulized test group C1, the nebulized comparison group E1 to E6, and the non-nebulized test group D were subjected to a forced swim test after 15 minutes of nebulized or non-nebulized inhalation of distilled water or essential oil. The test was then completed after a 15-minute rest. The positive drug group (caffeine) was subjected to a forced swim test 30 minutes after oral administration. The mice were familiarized with the environment for 2 minutes, and the cumulative swimming rest time of the mice was recorded within 8 minutes. When the mice floated on the water surface and their hind legs did not move, they were in a static state. The test lasted for a total of 10 minutes. The results are as follows: Figure 19 and Figure 20 As shown, * indicates P < 0.05, ** indicates P < 0.01, and # indicates P < 0.05.

[0202] The forced swimming test is an effective method for creating a physical fatigue model. The forced swimming test was used to observe and record the swimming rest time of mice and investigate the effect of inhaling the essential oil composition on alleviating physical fatigue in mice. Figure 19 As shown, compared with the control group A, the positive drug group B, the nebulized test group C1, and the non-nebulized test group D significantly shortened the swimming rest time of mice (P<0.05), indicating that the essential oil composition and caffeine of Example 1 of the present invention can promote the autonomous activity of physically fatigued mice and improve physical fatigue.

[0203] like Figure 20 As shown, compared with the nebulization test group C1, the swimming rest time of mice in groups E1 to E6 was significantly increased (P<0.05), indicating that the lack of any essential oil in the essential oil composition and the lack of borneol or menthol in the low eutectic composition would significantly reduce the wakefulness-promoting effect, and it was shown that the promoting effect of the essential oil composition was the result of the synergistic effect of each component therein.

[0204] 4. Multiple-dose open field test

[0205] After the forced swimming test, the mice were given the drug for 5 days. On the fifth day of the test, the open field test was performed. The control group A, the nebulized test group C1, the nebulized comparison group E1 to E6, and the non-nebulized test group D inhaled distilled water or essential oil for 15 minutes each day. After taking the drug for 15 minutes, they rested for 15 minutes on the fifth day and then performed the open field test. The positive drug group was gavaged with 57 mg / kg of caffeine every day. On the fifth day, the open field test was performed 30 minutes after gavage. The mice were familiarized with the environment for 3 minutes, and the behavioral parameters within 5 minutes were recorded: total movement distance (cm) and average speed (cm / s). The results are as follows: Figures 21 to 26 As shown, * indicates P < 0.05, ** indicates P < 0.01, and # indicates P < 0.05.

[0206] like Figure 21 、 Figure 22 、 Figure 23 As shown, compared with the control group A, the positive drug group B had no significant effect on the movement distance and average speed of the mice (P>0.05), and the nebulization test group C1 and the non-nebulization test group D could significantly increase the movement distance and average speed of the mice (P<0.05), and shorten the rest time (P<0.05), indicating that the essential oil composition of Example 1 of the present invention can provide long-lasting stimulation when inhaled, is not easily drug-tolerant, and has a better effect than caffeine.

[0207] like Figure 24 、 Figure 25 、 Figure 26 As shown, compared with the aerosol test group C1, the movement distance and average speed of mice in groups E1 to E6 were reduced (P < 0.05), and the rest time was increased (P < 0.05). This indicates that the lack of any essential oil in the essential oil composition, and the lack of borneol or menthol in the eutectic composition, significantly reduced the promoting effect on the autonomous movement of mice, and that the promoting effect of the essential oil composition of the present invention is the result of the synergistic effect of its components.

[0208] The open field test is a commonly used test method for evaluating an animal's autonomous activity ability, ability to explore unfamiliar environments, and level of tension; movement distance, average speed, and rest time are commonly used criteria for evaluating the nerve impulses, excitability, and activity of mice; the forced swim test utilizes the mouse's fear of water. In this environment, the animal is always in a state of escape but unable to escape. It is often used to evaluate the effectiveness of drug treatment for depression, and also reflects the degree of animal excitability and physical fatigue.

[0209] The present invention investigated the refreshing effect of the essential oil composition of the present invention on normal mice after inhalation using open field tests, forced swim tests, and sodium amobarbital sleep-induction tests. The results of the open field tests showed that single or repeated administration of the essential oil composition of the present invention significantly improved the mice's ability to move autonomously, as demonstrated by increased movement distance and average speed. Furthermore, compared with the positive drug (caffeine), the essential oil composition of the present invention exhibited a more pronounced and sustained effect in improving the mice's ability to move autonomously. The results of a suprathreshold dose of sodium amobarbital sleep-induction test showed that the essential oil composition of the present invention significantly shortened the mice's sleep duration, demonstrating a wakefulness-promoting effect.

[0210] The above test results show that inhaling the essential oil composition of the present invention can effectively promote the autonomous activity of normal mice, shorten the sleep-inducing time of sodium amobarbital, and promote wakefulness. It can also relieve physical fatigue, exert a refreshing effect, and is not easily tolerated.

[0211] The above-described embodiments merely illustrate several implementations of the present invention, and while their descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the present invention. It should be noted that a person skilled in the art would be able to make numerous variations and improvements without departing from the spirit of the present invention, all of which fall within the scope of protection of the present invention. Therefore, the scope of protection of the present invention shall be determined by the appended claims.

Claims

1. An anti-fatigue essential oil composition, characterized in that, The essential oil composition comprises a compound essential oil and a eutectic composition; The compound essential oil comprises the following essential oil components in parts by weight: 1 to 25 parts of Torreya grandis aril essential oil; 1 to 20 parts of cinnamon leaf essential oil; 1 to 20 parts of lemon essential oil; 1 to 15 parts of ginger essential oil; The eutectic composition comprises the following raw materials in parts by weight: 1 to 3 parts borneol; 1 part to 10.5 parts of menthol; The method comprises adding a potassium citrate solution to the torreya aril, squeezing the torreya aril into juice, and then distilling the torreya aril essential oil; Adding sodium acetate solution to cinnamon leaves, squeezing into juice, and distilling to obtain the cinnamon leaf essential oil; Adding potassium citrate solution to fresh lemons, squeezing the juice, and then distilling to obtain the lemon essential oil; Purified water is added to ginger, the ginger is squeezed into juice, and then the ginger essential oil is obtained by distillation; The compound essential oil is evenly mixed with the melted eutectic composition to obtain the essential oil composition.

2. An anti-fatigue essential oil composition according to claim 1, characterized in that, In the essential oil composition, the compound essential oil comprises the following essential oil components in parts by weight: 20-25 parts of Torreya grandis aril essential oil; 15-20 parts cinnamon leaf essential oil; 15-20 parts of lemon essential oil; 10-15 parts of ginger essential oil; The eutectic composition comprises the following raw materials in parts by weight: 2~3 parts borneol; 7 to 10.5 parts of menthol.

3. An anti-fatigue essential oil composition according to claim 1, characterized in that, In the compound essential oil, according to the weight ratio, Torreya grandis aril essential oil: cinnamon leaf essential oil: lemon essential oil: ginger essential oil = (4-5): (3-4): (3-4): 3; In the eutectic composition, the weight ratio of borneol to menthol is 1:3-4.

4. An anti-fatigue essential oil composition according to claim 1, characterized in that, In the essential oil composition, the mass ratio of the eutectic composition is 10% to 15%.

5. An anti-fatigue essential oil composition according to claim 3, characterized in that, In the compound essential oil, according to the weight ratio, Torreya grandis aril essential oil: cinnamon leaf essential oil: lemon essential oil: ginger essential oil = 5:4:4:3; In the eutectic composition, the weight ratio of borneol to menthol is 1:3.

5.

6. The method for preparing the anti-fatigue essential oil composition according to any one of claims 1 to 5, characterized in that: The preparation method comprises the following steps: The cleaned Torreya grandis aril, cinnamon leaves, fresh lemon and ginger are granulated or sliced ​​respectively; The treated Torreya grandis aril is squeezed, and potassium citrate solution is added during the squeezing process to obtain Torreya grandis aril juice; The processed cinnamon leaves are squeezed, and sodium acetate solution is added during the squeezing process to obtain cinnamon leaf juice; The processed fresh lemons are squeezed, and potassium citrate solution is added during the squeezing process to obtain lemon juice; The processed ginger is squeezed, and purified water is added during the squeezing process to obtain ginger juice; Torreya grandis aril juice, cinnamon leaf juice, lemon juice, and ginger juice are respectively added to corresponding steam distillation devices and heated, and fractions are taken out and dried to obtain Torreya grandis aril essential oil, cinnamon leaf essential oil, lemon essential oil, and ginger essential oil, respectively; Torreya grandis aril essential oil, cinnamon leaf essential oil, lemon essential oil and ginger essential oil are mixed in proportion to obtain a compound essential oil; The compound essential oil is evenly mixed with the melted eutectic composition to obtain the essential oil composition.

7. The method for preparing an anti-fatigue essential oil composition according to claim 6, wherein The steps of uniformly mixing the compound essential oil with the melted eutectic composition to obtain the essential oil composition are as follows: Mixing borneol and menthol in a container, heating to ≤50°C to completely melt them, and stirring for more than 20 minutes to obtain a eutectic composition; Torreya grandis aril essential oil, cinnamon leaf essential oil, lemon essential oil and ginger essential oil are added to the eutectic composition, and the mixture is stirred and mixed evenly.

8. The method for preparing an anti-fatigue essential oil composition according to claim 6, wherein In the preparation of the Torreya grandis aril juice, the ratio of Torreya grandis aril to potassium citrate solution is 10:6-8 by weight, and the pH of the potassium citrate solution is 7.5-8.5; When preparing the cinnamon leaf juice, the ratio of cinnamon leaf to sodium acetate solution is 10:10-12, and the pH value of the sodium acetate solution is 7.5-8.5; When preparing the lemon juice, the ratio of fresh lemon to potassium citrate solution is 10:4-6, and the pH value of the potassium citrate solution is 8-9; When preparing the ginger juice, the ratio of ginger to purified water is 10:4-6.

9. The method for preparing an anti-fatigue essential oil composition according to claim 6, wherein When preparing the Torreya grandis aril essential oil, the heating temperature in the steam distillation apparatus is 90° C. to 100° C., the pressure is 300 mbar to 500 mbar, and the heating time is 2 h to 4 h; When preparing the cinnamon leaf essential oil, the heating temperature in the steam distillation apparatus is 70° C. to 100° C., the pressure is 300 mbar to 500 mbar, and the heating time is 3 h to 5 h; When preparing the lemon essential oil, the heating temperature in the steam distillation apparatus is 90° C. to 100° C., the pressure is 300 mbar to 500 mbar, and the heating time is 2 h to 4 h; When preparing the ginger essential oil, the heating temperature in the steam distillation device is 80° C. to 100° C., the pressure is 300 mbar to 500 mbar, and the heating time is 2 h to 4 h.

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