Microfluidic device based on MMPs-DNAzyme-PMPs compound and detection method

Through the MMPs-DNAzyme-PMPs complex microfluidic device, combined with automated capillary drive, rapid quantitative detection of blood calcium ions without complex instruments is achieved, and the problems of complex operation and unstable storage in the prior art are solved, and are suitable for real-time detection at home and medical sites.

CN120460039APending Publication Date: 2025-08-12GUANGDONG MEDICAL UNIV
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Patent Information

Application Number
CN202510731756.1
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-06-03
Publication Date
2025-08-12

AI Technical Summary

Technical Problem

The existing blood calcium detection methods are complex in operation and require professional instruments and precision optical equipment, so they cannot achieve instant detection in non-laboratory environments. Traditional microfluidic chips are susceptible to the influence of ambient temperature and humidity, resulting in reagent deactivation or microchannel blockage.

Method used

Using a microfluidic device of MMPs-DNAzyme-PMPs complex, DNAzyme releases PMPs in the presence of calcium ions, combined with automated capillary drive, PMPs are captured through the pore size difference at the end of the particle dam to form visual bands, realizing quantitative detection of the naked eye.

Benefits of technology

It realizes rapid blood calcium ion detection without complex instruments, reduces equipment costs, improves storage stability and detection sensitivity, and is suitable for real-time inspections at home and medical sites.

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Abstract

The invention discloses a microfluidic device based on an MMPs-DNAzyme-PMPs compound and a detection method, and belongs to the technical field of biological detection. According to the invention, DNAzyme molecules are combined with magnetic particles (MMPs) and polystyrene particles (PMPs), and the obtained composite structure (MMPs-DNAzyme-PMPs) can trigger a DNAzyme cutting reaction in the presence of calcium ions, so that free PMPs are released. In combination with an automatic capillary tube driving micro-fluidic device, the PMPs are captured and accumulated by utilizing the characteristic that the aperture of the tail end of a particle dam is smaller than the diameter of the free PMPs, a visual length strip is formed, and rapid quantitative detection of the blood calcium ion concentration can be realized through naked eyes or a mobile phone camera. The enrichment and concentration visualization of the calcium ions can be realized only by using a trace amount of blood sample, and the method is suitable for instant detection of families and medical sites.
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