DEHP degrading bacterium with nitrogen and phosphorus removal effect and application of DEHP degrading bacterium

Abstract

The invention discloses a DEHP degrading bacterium with nitrogen and phosphorus removal effects and application of the DEHP degrading bacterium. According to the invention, an Acinetobacter pittii 25S0261-6 strain is separated from estuary sediments of wastewater, the strain is preserved in Guangdong Microbial Culture Collection Center on April 7, 2025, and the preservation number is GDMCC No: 66104. The invention also discloses a method for preparing the Acinetobacter pittii 25S0261-6 strain. Researches show that the 25S0261-6 strain has the effect of efficiently degrading DEHP, can synchronously remove nitrogen and phosphorus while degrading DEHP, also has certain salt tolerance, has the DEHP degradation rate of 80% or above under high salt concentration, and can be applied to nitrogen and phosphorus removal in coastal sewage and restoration of plasticizer pollution. The strain 25S0261-6 enriches the strain resources of DEHP degrading bacteria, and provides more efficient strains and remediation methods for degradation, dephosphorization and denitrification of DEHP in coastal polluted water areas.

Description

Technical Field

[0001] The present invention belongs to the technical field of microbial degradation and pollution environment treatment, and more specifically relates to a DEHP-degrading bacterium with denitrification and dephosphorization functions and its application. Background Art

[0002] With the continuous development of industry, the discharge of industrial wastewater and untreated sewage into the natural and domestic environments through water bodies has caused a series of environmental problems, resulting in serious water pollution. On the one hand, some persistent pollutants, such as di(2-ethylhexyl) phthalate (DEHP) in plastic products, are easily transferred into the environment, causing environmental pollution such as water, soil, and air pollution. Once DEHP enters the environment, it is difficult to degrade and can persist for a long time, causing adverse effects on the environment and organisms. It can also harm humans through food, breathing, and skin contact, accumulating through the food chain and ultimately enriching in the human body, threatening intergenerational health. On the other hand, groundwater pollution and nitrogen and phosphorus compounds can cause eutrophication of water bodies, leading to excessive algae growth, a sudden drop in dissolved oxygen, and the suffocation and death of fish, causing serious environmental pollution. Therefore, it is extremely necessary to find a method to effectively remove or purify pollutants in water.

[0003] Among existing methods for remediating environmental pollutants, physical remediation is difficult to implement on a large scale due to its enormous workload, consuming significant manpower, material, and financial resources. Chemical remediation is simple to operate, but its economic cost is high and it may generate other harmful substances, which are prone to secondary pollution and pose potential environmental risks, requiring further treatment. Microbial remediation can completely mineralize pollutants without causing secondary pollution, and is economical, costing only 30-50% of the cost of traditional chemical and physical remediation. It is also simple to operate, safe, friendly, and has minimal impact on the environment, making it the most important technology for removing pollutants.

[0004] At present, the degradation of DEHP by microorganisms mainly focuses on the isolation, identification and degradation characteristics of degrading bacteria. In recent years, many researchers have screened and isolated a variety of microplastic and plasticizer-degrading bacteria from soil, sediments, riverbed sludge, wetlands and other environments, such as Pseudomonas aeruginosa ( Pseudomonas ), Gordonia ( Gordonia ), Arthrobacter ( Arthrobacter ), Rhodococcus ( Rhodococcus ), and Achromobacterium ( Achromobacter ) etc. Studies have also found that microorganisms with denitrification and phosphorus removal functions, such as Azovibrio ( Azoarcus ), Bacillus subtilis ( Bacillus subtilis ) and are widely used in the restoration of large-scale polluted waters.

[0005] To date, numerous studies have reported on strains capable of either single-use DEHP degradation or nitrogen and phosphorus removal. However, relatively few bacteria can simultaneously remove both DEHP and nitrogen and phosphorus from polluted water. Given the coexistence of DEHP and nitrogen and phosphorus in estuaries, effective simultaneous removal of these two pollutants has become a key focus. Therefore, identifying bacteria with both DEHP removal and DEHP performance, and applying them to domestic and industrial wastewater discharge outlets at estuaries, will effectively reduce the toxic effects of both on the environment and provide scientific support for the ongoing coordinated land-sea pollution prevention and control efforts to control the total amount of pollutants entering the sea, as well as for ecological and environmental protection. Summary of the Invention

[0006] The technical problem to be solved by the present invention is to overcome the shortcomings of existing degradation bacteria that can simultaneously remove DEHP and nitrogen and phosphorus in polluted water, and to provide a DEHP degradation bacteria with denitrification and dephosphorization functions and its application.

[0007] The first object of the present invention is to provide a strain of Acinetobacter pituitus ( Acinetobacter pittii )25S0261-6 strain.

[0008] The second object of the present invention is to provide the use of the Acinetobacter pitei 25S0261-6 strain and / or bacterial liquid.

[0009] The third object of the present invention is to provide a DEHP degradation agent or a phosphorus and nitrogen removal preparation.

[0010] The fourth object of the present invention is to provide a method for degrading DEHP and / or removing phosphorus and nitrogen.

[0011] The fifth object of the present invention is to provide a method for repairing polluted coastal waters.

[0012] The above-mentioned purpose of the present invention is achieved through the following technical solutions: The present invention provides a strain of Acinetobacter pituitus ( Acinetobacter pittii Strain 25S0261-6 was deposited with the Guangdong Provincial Center for Microbiological Culture Collection on April 7, 2025, under the GDMCC No. 66104. The address of the collection is 5th Floor, Building 59, No. 100, Xianlie Middle Road, Guangzhou. Colonies of strain 25S0261-6 are yellow, opaque, round, with a raised surface, distinct edges, and a moist, glossy appearance. They are Gram-negative and oxidase-negative, but positive for catalase and catalase. Suitable culture conditions for strain 25S0261-6 include a pH range of 6-9, a temperature range of 20-40°C, and a salt concentration range of 2%-8%.

[0013] Research has shown that strain 25S0261-6 can remove phosphorus and nitrogen, and degrade DEHP. The strain's degradation rate reached 95.4%, and even reached 92.97%-80.87% at salinity levels between 2% and 6%. The strain also exhibits a degree of salt tolerance, making it suitable for use in salt-stressed environments. Furthermore, after 72 hours, strain 25S0261-6 achieved nitrite removal rates of 95.4%, nitrate removal rates of 96.6%, ammonia removal rates of 95.7%, and phosphorus removal rates of 94.75%-96%, demonstrating its high efficiency in removing ammonium, nitrate, nitrite, and phosphorus from polluted waters. Furthermore, under highly saline and alkaline conditions, the strain can promote the degradation of DEHP and phosphorus and nitrogen removal in polluted coastal waters, providing a method and scientific basis for the simultaneous removal of phosphorus and nitrogen from DEHP in these areas.

[0014] Therefore, the present invention provides the use of the 25S0261-6 strain in degrading DEHP, or removing phosphorus and nitrogen, or simultaneously degrading DEHP and removing phosphorus and nitrogen.

[0015] The present invention provides the use of the 25S0261-6 strain and / or bacterial liquid in degrading DEHP or removing phosphorus and nitrogen in a high-salt environment.

[0016] Preferably, the high salt environment has a salt concentration of 2-8%.

[0017] The present invention provides use of the 25S0261-6 strain and / or bacterial liquid in preparing a DEHP degrading agent or in preparing a dephosphorus and denitrification preparation.

[0018] The present invention provides the use of the 25S0261-6 strain and / or bacterial liquid in repairing polluted coastal water bodies.

[0019] Preferably, the coastal pollution refers to DEHP pollution and / or nitrogen and phosphorus pollution.

[0020] The present invention provides the use of the 25S0261-6 strain and / or bacterial liquid in removing phosphorus and nitrogen from coastal polluted water bodies, or in degrading DEHP in coastal polluted water bodies.

[0021] Preferably, the polluted coastal water body is a high saline-alkali environment.

[0022] The invention provides a DEHP degradation agent or a dephosphorus and denitrification preparation, wherein the preparation contains a 25S0261-6 bacterial strain and / or a bacterial liquid.

[0023] Preferably, the concentration of 25S0261-6 strain and / or bacterial solution in the preparation is not less than 1×10 8 cfu / mL.

[0024] The invention provides a method for degrading DEHP and / or removing phosphorus and nitrogen, which adopts 25S0261-6 bacterial strain and / or bacterial liquid for treatment.

[0025] The present invention also provides a method for repairing polluted coastal waters, which uses the 25S0261-6 strain and / or bacterial liquid, or the above-mentioned preparation to treat the polluted waters.

[0026] The present invention has the following beneficial effects: The present invention isolated a strain of Acinetobacter pituitus that has the functions of degrading DEHP and removing phosphorus and nitrogen. Acinetobacter pittii The 25S0261-6 strain, which degrades DEHP and also exhibits aerobic phosphorus and nitrogen removal properties, can degrade DEHP by over 80% at salinity levels of 2-8%. It can simultaneously remove phosphorus and nitrogen and degrade DEHP in highly saline and alkaline coastal polluted waters, making it particularly suitable for denitrification and phosphorus removal in coastal wastewater and for preventing and controlling plasticizer pollution. The 25S0261-6 strain provided by the present invention provides additional methods for degrading DEHP and removing phosphorus and nitrogen from polluted coastal waters, and also provides scientific support for the ongoing coordinated land-sea pollution prevention and control efforts to control the total amount of pollutants entering the sea, as well as for ecological and environmental protection. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 This is the morphological characteristic diagram of the 25S0261-6 strain.

[0028] Figure 2 This is the phylogenetic tree of strain 25S0261-6.

[0029] Figure 3 This is the result diagram of the optimal growth conditions of the 25S0261-6 strain (where a is the effect of pH on strain growth; b is the effect of temperature on strain growth; c is the effect of salinity on strain growth; d is the effect of inoculation size on strain growth).

[0030] Figure 4 Figure 2 is the DEHP degradation rate of strain 25S0261-6 and the DEHP degradation diagram under different salinities (where a is the DEHP degradation rate at different times; b is the DEHP degradation rate at different salinities).

[0031] Figure 5 This is a graph of the phosphorus removal and nitrogen removal degradation rates of the 25S0261-6 strain (where a is nitrite nitrogen and phosphorus removal rate; b is nitrate nitrogen and phosphorus removal rate; c is ammonia nitrogen and phosphorus removal rate).

[0032] Figure 6 It is the performance of 25S0261-6 strain in simultaneous nitrogen and phosphorus removal under DEHP pollution conditions. DETAILED DESCRIPTION

[0033] The present invention will be further described below with reference to the accompanying drawings and specific examples, but the examples do not limit the present invention in any way. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in the art.

[0034] Unless otherwise specified, all reagents and materials used in the following examples were commercially available.

[0035] The culture medium formulations used in the following examples are as follows: Enrichment medium: (NH4)2SO4 1.0 g, KH2PO4 0.2 g, MgSO4·7H2O 1.6 g, NaCl 8 g, FeSO4·7H2O 0.01 g, CaCl·2H2O 0.02 g, pH = 7.0 ± 0.2.

[0036] LB medium: peptone 10.0 g, NaCl 5.0 g, yeast extract 10.0 g, solid medium plus agar 20 g, pH = 7.0 ± 0.2.

[0037] HNM medium: C6H5Na3O7·2H2O 5.0 g, (NH4)2SO4 0.05 g, NaCl 0.01 g, K2HPO4 0.2 g, MgSO4·7H2O 0.05 g, FeSO4·7H2O 0.01 g, MnSO4 0.01 g, pH = 7.0 ± 0.2, trace element solution 1 ml / L.

[0038] SDM medium: C6H5Na3O7·2H2O 5.0 g, KNO2 0.025 g, NaCl 0.01 g, K2HPO4 0.2 g, MgSO4·7H2O 0.05 g, FeSO4·7H2O 0.01 g, MnSO4 0.01 g, pH = 7.0 ± 0.2, trace element solution 1 ml / L.

[0039] DM culture medium: C6H5Na3O7·2H2O 5.0 g, NaNO3 0.08 g, NaCl 0.01 g, K2HPO4 0.2 g, MgSO4·7H2O 0.05 g, FeSO4·7H2O 0.01 g, MnSO4 0.01 g, pH = 7.0±0.2, trace element solution 1 ml / L.

[0040] Example 1 Isolation and characterization of strains 1. Isolation and purification of strains The sample was obtained from wastewater estuary sediments through enrichment, separation and purification, which specifically included the following steps: (1) Enrichment of strains: Accurately weigh 10g of soil sample and place it in a 250mL Erlenmeyer flask containing 90mL of sterile water (with glass beads). Shake on a shaker for 30min to fully mix the sample with water, disperse the cells, and form a uniform bacterial suspension. Let it stand for 20-30s, then pipette 2mL of supernatant into an inorganic salt medium containing 100mg / L DEHP (with DEHP as the only nitrogen source) and culture in the dark at 30℃ and 180rpm. After culturing for 5 days, inoculate again with 2% inoculum into an inorganic salt medium containing 200mg / L DEHP and culture under the same conditions. Repeat this process until the DEHP concentration in the inorganic salt medium reaches 500mg / L. Each enrichment culture has a blank control without adding bacterial solution.

[0041] (2) Separation and purification: The enriched culture fluid was washed with sterile water at 10 -1 , 10 -2 , 10 -3 , gradient dilution, and then evenly spread the dilutions of different gradients on the solid culture medium containing DEHP (100 mg / L), place them in a constant temperature incubator at 30℃ in the dark, observe the morphological characteristics of the colonies, pick single bacteria with different morphologies and further separate and purify them on the solid culture medium, and obtain pure strains after multiple streaking purification cultures.

[0042] 2. Screening of degradation strains A certain amount of the acclimated bacterial suspension was inoculated in an inorganic salt culture medium serving as a single carbon source and cultured at 30°C with shaking at 200 rpm. Every 8 hours, the DEHP content before and after incubation was determined using ultra-performance liquid chromatography (UPLC), and the degradation rate was calculated. A blank control (no inoculation of the acclimated strain into the inorganic salt culture medium) was also established to eliminate the effects of physical and chemical factors. Preliminary screening identified the acclimated strain with the highest degradation rate as a highly efficient DEHP-degrading strain, designated 25S0261-6.

[0043] 3. Identification of strain characteristics (1) Morphological characteristics of the strain: The purified 25S0261-6 strain was streaked on LB solid medium and cultured at 30°C for 24 h. The colonies were yellow, opaque, round, with a convex surface, clear edges, moist and shiny. Figure 1 shown.

[0044] (2) Physiological and biochemical characteristics of the strain: Physiological and biochemical characteristics showed that the 25S0261-6 strain was Gram-negative and oxidase-negative, but positive for catalase and catalase. (3) Molecular biological characteristics: Total bacterial DNA was extracted using a kit, and 16S rDNA was amplified by PCR using universal primers 27F and 1492R. A clear band appeared around 1400 bp. The PCR amplification product was recovered and sequenced. The obtained DNA sequence was then subjected to Blast analysis on the NCBI website (http: / / www.ncbi.nlm.nih.gov), and a phylogenetic tree was constructed using MEGA software (version: 11.0).

[0045] The results are as follows Figure 2 As shown, it was found that the 16S rDNA sequence of the 25S0261-6 strain isolated by the present invention was similar to that of Acinetobacter pittii strain FI1-01 (MK396586.1) has a high homology, and the nucleotide sequence of 16S rDNA of strain 25S0261-6 is shown in SEQ ID NO: 1. Combined with the above morphological characteristics, physiological and biochemical characteristics, and 16SrDNA sequence results, strain 25S0261-6 was taxonomically classified as Acinetobacter pituitarius ( Acinetobacter pittii ), named as strain 25S0261-6, and deposited in Guangdong Provincial Microbiological Culture Collection on April 7, 2025. The strain collection number is GDMCC No: 66104, and the storage address is 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou.

[0046] Example 2 Test of the optimal growth conditions of strain 25S0261-6 1. Effect of pH on strain growth The seed solution of 25S0261-6 was heated to (OD 600 =0.8) was inoculated into LB medium at a 1% inoculum size, and the pH was set to 5, 6, 7, 8, 9, and 10, respectively. Three replicates were set, and the culture was shaken at 200 r / min and 28°C. After culturing for 24 h, samples were taken to determine the growth of the strain (OD 600 ).

[0047] 2. Effect of temperature on strain growth The seed solution of 25S0261-6 was heated to (OD 600=0.8) was inoculated into LB medium at a 1% inoculum size, and the temperatures were set at 10°C, 20°C, 30°C, 40°C, and 50°C, respectively. Three replicates were set, and the culture was shaken at 200 r / min at different temperatures. After culturing for 24 h, samples were taken to determine the growth of the strain (OD 600 ).

[0048] 3. Effect of salinity on strain growth The seed solution of 25S0261-6 was heated to (OD 600 =0.8) was inoculated into LB medium at a 1% inoculum size, and the salinity was set to 2%, 4%, 6%, 8%, and 10%, respectively. Three replicates were set, and the culture was shaken at 200 r / min and 28°C under constant temperature. After culturing for 24 h, samples were taken to determine the growth of the strain (OD 600 ).

[0049] 4. Effect of inoculum size on strain growth The seed solution of 25S0261-6 was heated to (OD 600 =0.8) were inoculated into LB medium at 1%, 3%, 5%, 7%, and 9% inoculum, with 3 replicates, and cultured at 200 r / min and 28°C in a constant temperature shaking culture. After culturing for 24 h, samples were taken to determine the growth of the strain (OD 600 ).

[0050] The statistical results of the growth conditions of the above strains are as follows Figure 3 As shown, the suitable culture conditions for strain 25S0261-6 are: pH range 6-9 ( Figure 3 (a)), temperature range 20-40℃ ( Figure 3 (b)), salt concentration range 2%-8% ( Figure 3 (c)), the inoculation range is 1-10% ( Figure 3 (d)).

[0051] Example 3 Effect of application of strain 25S0261-6 in coastal polluted waters 1. Preparation of 25S0261-6 bacterial agent 50 μL of 25S0261-6 bacterial suspension was added to 150 mL of sterilized LB liquid medium and cultured at 200 rpm and 28°C for 12 h. 1% inoculum was inoculated into 150 mL of LB medium and cultured at 30°C and 180 rpm for 1 day. The culture was then centrifuged at 8000 rpm for 10 min, the cells were suspended in sterile water, centrifuged twice to wash away the culture medium, and the suspension was resuspended in sterile water to a concentration of 1×10 8 cfu / mL of bacterial agent.

[0052] 2. Degradation effect of strains on DEHP in coastal polluted waters In order to observe the degradation effect of Acinetobacter pitei 25S0261-6 on DEHP in coastal polluted waters, two treatment groups were set up in this experiment: (1) blank control: no addition of bacterial agent 25S0261-6; (2) 25S0261-6 treatment: addition of 100 mL 1×10 8 The strain 25S0261-6 was cultured at a concentration of 100 cfu / mL, with three replicates per treatment. The DEHP removal capacity of strain 25S0261-6 in polluted coastal waters was evaluated by varying DEHP concentrations. Ultra-performance liquid chromatography (UPLC) was used to determine the DEHP content in the culture and calculate the degradation rate.

[0053] 3. Performance test of 25S0261-6 strain in degrading DEHP under different salinity conditions Aspirate 25S0261-6 bacterial solution (OD 600 =0.8) was inoculated into inorganic salt ion culture medium at a 3% inoculum size. The salinity was set to 2%, 4%, 6%, 8%, and 10%, respectively. 0% was set as the blank control. Three replicates were set. The culture was shaken at 200 r / min and 28°C. After 48 h, 200 μL of the bacterial solution was collected and the DEHP content in the culture was determined by ultra-performance liquid chromatography (UPLC) and the degradation amount was calculated.

[0054] The results of the test are as follows Figure 4 As shown in (a), after 48 hours, the degradation rate of DEHP by strain 25S0261-6 was 95.4%. Figure 4 As shown in (b), strain 25S0261-6 showed the best DEHP degradation, with degradation rates ranging from 92.97% to 80.87% at salinity levels of 2% to 6%. When salinity levels increased to 8% and 10%, the degradation rates reached 60.03% and 42.57%, respectively. This indicates that strain 25S0261-6 is capable of degrading DEHP under high-salinity conditions and can be used to degrade DEHP in coastal saline soils.

[0055] Example 4 Phosphorus and Nitrogen Removal Performance Test of Strain 25S0261-6 Aspirate 25S0261-6 bacterial solution (OD 600 =0.8) were inoculated into 150 mL of HNM medium, SDM medium, and DM medium at a 3% inoculum size, with the initial TN concentration set at 150 mg / L and the initial TP concentration set at 80 mg / L. The culture was then incubated at 200 r / min and 28°C for 72 h. The NH4 - N, NO3 - N, NO2 -N and PO4 - The residual content of P was calculated by taking the culture medium of uninoculated strain as blank control and setting 3 replicates for each experiment. - N, NO3 - N, NO2 - N and PO4 - P removal rate.

[0056] The results of the test are as follows Figure 5 As shown in (a), after the strain was cultured at constant temperature for 72 hours, the removal rate of nitrite nitrogen was 95.4% and the removal rate of phosphorus was 96%. Figure 5 As shown in (b), the removal rate of nitrate nitrogen is 96.6%, and the removal rate of phosphorus is 94.75%. Figure 5 As shown in (c), the ammonia nitrogen removal rate was 95.7%, and the phosphorus removal rate was 95.1%, indicating that the 25S0261-6 strain had good phosphorus and nitrogen removal performance and could efficiently remove ammonium nitrogen, nitrate nitrogen, nitrite nitrogen, and phosphorus.

[0057] Example 5 Simultaneous DEHP and Phosphorus and Nitrogen Removal Performance Test of Strain 25S0261-6 Absorb the seed solution of 25S0261-6 strain (OD 600 =0.8) was inoculated at a 3% rate into synthetic wastewater with initial concentrations of 100 mg / L for DEHP and 100 mg / L for TN and 40 mg / L for TP. The culture was incubated at 200 rpm and 28°C for 72 h. The residual contents of DEHP, TN, and TP in the culture medium were determined every 12 h. The culture medium without the inoculated strain was used as the blank control. Three replicates were set for each experiment, and the removal efficiencies of DEHP, TN, and TP were calculated.

[0058] The results are as follows Figure 6 As shown, after constant temperature culture for 72 hours, the 25S0261-6 strain had a DEHP removal rate of 87.6%, a TN removal rate of 80.6%, and a TP removal rate of 81.3%, which showed that it had the effect of efficiently degrading DEHP and removing nitrogen and phosphorus.

[0059] Example 6: DEHP, phosphorus and nitrogen removal performance test of strain 25S0261-6 at the actual estuary Due to its excellent DEHP removal and nitrogen and phosphorus removal capabilities, strain 25S0261-6 was used to remediate estuarine sediment-contaminated water bodies high in nitrate nitrogen and DEHP. The contaminated water used in this example was collected from the sea near the Nanliu River estuary in Zhanjiang City, Guangdong Province. The contaminated water had the following properties: pH 8.3, salinity 3.7%, TN content 61.6 mg / L, TP concentration 24.5 mg / L, and DEHP concentration 90 mg / L. A 3% inoculum of strain 25S0261-6 seed solution (OD600 = 0.8) was inoculated into 100 mL of estuarine water contaminated with high salinity, nitrate nitrogen, and DEHP. A blank control was also established, and the DEHP, TN, and TP removal rates were calculated.

[0060] The test results are shown in Table 1. After 48 hours, the TN removal rate of strain 25S0261-6 in the polluted water of the estuary was 65.75%, the TP removal rate was 70.36%, and the DEHP degradation rate was 80.42%.

[0061] Table 1 Treatment of polluted water bodies in estuaries with and without inoculation

[0062] In summary, the present invention isolated a strain of Acinetobacter pituitarius ( Acinetobacter pittii The 25S0261-6 strain is highly effective in degrading DEHP and exhibits aerobic phosphorus and nitrogen removal. At salt concentrations of 2-8%, it can degrade DEHP by over 80%, demonstrating excellent salt tolerance and suitability for DEHP degradation in salt-stressed environments. The 25S0261-6 strain has been used in highly saline and alkaline coastal polluted waters, simultaneously removing phosphorus and nitrogen and degrading DEHP. Its TN removal rate in polluted estuaries was 65.75%, TP removal was 70.36%, and DEHP degradation was 80.42%. This strain is well-suited for nitrogen and phosphorus removal in coastal wastewater and for preventing and controlling plasticizer pollution. Using the 25S0261-6 strain to prepare a degrading agent facilitates scale-up and expansion, enriching the pool of DEHP-degrading strains. This provides additional methods for DEHP degradation, phosphorus removal, and nitrogen removal in polluted coastal waters, and provides scientific support for coordinated land-sea pollution prevention and control, as well as ecological and environmental protection, to control the total amount of pollutants entering the sea.

[0063] The above embodiments are preferred implementation modes of the present invention, but the implementation modes of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications that do not deviate from the spirit and principles of the present invention should be considered as equivalent replacement methods and are included in the scope of protection of the present invention.

Claims

1. A strain of Acinetobacter pituitii ( Acinetobacter pittii ) 25S0261-6 strain, characterized in that The strain was deposited in Guangdong Provincial Microbiological Culture Collection Center on April 7, 2025, with the collection number GDMCC No: 66104.

2. Use of the strain according to claim 1 in degrading DEHP and / or removing phosphorus and nitrogen.

3. Use of the 25S0261-6 strain and / or bacterial solution according to claim 1 in preparing a DEHP degrading agent or in preparing a phosphorus and nitrogen removal preparation.

4. Use of the 25S0261-6 strain and / or bacterial solution described in claim 1 in remediation of polluted coastal waters.

5. The application according to claim 4, characterized in that: The coastal pollution refers to DEHP pollution and / or nitrogen-containing wastewater pollution.

6. Use of the 25S0261-6 strain and / or its bacterial solution according to claim 1 in removing phosphorus and nitrogen from polluted coastal waters.

7. A DEHP degradation agent or a dephosphorization and denitrification preparation, characterized in that: The preparation contains the 25S0261-6 strain and / or bacterial liquid according to claim 1.

8. The preparation according to claim 7, characterized in that The concentration of the 25S0261-6 strain and / or bacterial solution in the preparation is not less than 1×10 8 cfu / mL.

9. A method for degrading DEHP and / or removing phosphorus and nitrogen, characterized in that: The treatment is carried out using the 25S0261-6 strain and / or bacterial solution described in claim 1.

10. A method for repairing polluted coastal waters, characterized in that: The preparation according to claim 7 or 8 is used to treat polluted waters.

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