Temperature-sensitive material for wound repair and application

Abstract

The invention belongs to the technical field of trauma repair, and particularly relates to a temperature-sensitive material for wound repair and application, the temperature-sensitive material for wound repair comprises a temperature-sensitive repair base material and a drug component for wound inflammation diminishing; the weight percentage ratio of the temperature-sensitive repairing base material to the medicine component is (60%-99.8%): (0.2%-40%), so that the weight percentage of the temperature-sensitive material is 100%. According to the technical scheme, the 3D exosome is added into the temperature-sensitive hydrogel to prepare the temperature-sensitive repair base material, the anti-inflammatory drug component is added into the temperature-sensitive repair base material, and the temperature-sensitive repair base material not only has the characteristics of the conventional temperature-sensitive hydrogel, but also adheres to the surface of a wound, provides stable drug supply for the surface of the wound, and has the performance of rapidly repairing the wound.

Description

Technical Field

[0001] The present invention belongs to the technical field of wound repair, and in particular relates to a temperature-sensitive material for wound repair and its application. Background Art

[0002] Clinical operations in relevant departments of current hospitals, such as obstetrics and gynecology, plastic surgery, and general surgery, all involve cutting the skin. After the operation is completed, drugs need to be used on the skin wound to avoid infection and improve the healing effect.

[0003] However, for some parts of the skin, because they are in a moist environment, the adhesion of the drugs is poor, resulting in a longer recovery time. For example, the recovery time for cervical surgery generally takes 4-6 weeks. Conventional gynecological products are easy to flow and shift away from the lesion site after being applied to the gynecological cervix or vagina. Moreover, if the patient moves upright after use, the product solution will flow out, which will not achieve the expected effect and cause a lot of trouble in life.

[0004] For external skin, the use of drugs is prone to poor adhesion due to friction and other reasons, and basically requires the use of bandages to avoid this. However, most of the drugs adhere to the gauze, and the amount of drugs that actually act on the wound is insufficient, resulting in poor wound recovery effect. Summary of the Invention

[0005] The purpose of the present invention is to provide a temperature-sensitive material and application for wound repair, which has good mucosal adhesion, will not cause drug leakage due to upright movement, and is not easy to adhere to other surfaces, thereby ensuring the amount of drug at the wound and solving the problems existing in the above-mentioned background technology.

[0006] A temperature-sensitive material for wound repair comprises a temperature-sensitive repair base material and a drug component for wound anti-inflammation; the weight percentages of the temperature-sensitive repair base material and the drug component are 60%-99.8%:0.2%-40%, so that the weight percentage composition of the temperature-sensitive material is 100%.

[0007] Furthermore, the weight percentage of the temperature-sensitive repair base material and the drug component is 80%-99%:1%-20%, so that the weight percentage composition of the temperature-sensitive material is 100%.

[0008] Furthermore, the thermosensitive material also includes a drug for increasing the speed of wound repair, which accounts for 1-10% of the thermosensitive material by weight.

[0009] Furthermore, the thermosensitive repair matrix includes a thermosensitive hydrogel and 3D exosomes, and the 3D exosomes are prepared by using 2D cells in a 3D culture system.

[0010] Furthermore, the thermosensitive repair base comprises 75%-90% of the thermosensitive hydrogel and the remainder of the 3D exosomes by weight.

[0011] Furthermore, the temperature-sensitive hydrogel is one or a combination of α-ketoglutaric acid, carboxyl chitin, hydroxypropyl chitin, hydroxybutyl chitosan, and hydroxypentyl chitosan.

[0012] An application, comprising the application of any of the above-mentioned thermosensitive materials for wound repair in wound repair.

[0013] The beneficial effects of the present invention are:

[0014] This technical solution adds 3D exosomes to a thermosensitive hydrogel to prepare a thermosensitive repair base and adds anti-inflammatory drug components. In addition to having the characteristics of conventional thermosensitive hydrogels, the thermosensitive repair base adheres to the wound surface, providing a stable supply of drugs to the wound surface and having the ability to quickly repair the wound. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 Schematic diagram of the initial model of Bama pigs, with a skin wound diameter of 2 cm, full-thickness skin lesions, and twice-weekly use.

[0016] Figure 2 This is a schematic diagram of the first wound healing experiment in Bama pigs. The diameter of the back skin wound is 1 cm and the wound depth is 0.5 cm.

[0017] Figure 3 This is a schematic diagram of the second use of the Bama pig wound healing experiment. DETAILED DESCRIPTION

[0018] The technical solutions of the present invention are described in detail below through examples. The following examples are merely exemplary and can only be used to explain and illustrate the technical solutions of the present invention, and cannot be interpreted as limiting the technical solutions of the present invention.

[0019] The materials involved in this application can all be obtained through commercial channels, and the sources of the materials are not described here.

[0020] In this application, the 3D exosomes of this application are prepared by using 2D cells in a 3D culture system, comprising the following steps:

[0021] This culture process uses Huaxin Bio 3D FloTrix ® The miniSpin exosome bioreactor has the following specific steps:

[0022] The bioreactor was wiped clean with 75% lees and placed in a CO2 incubator (5% CO2, 37°C) and connected to the power supply of the bioreactor.

[0023] Move four sterile 500 mL spinner flasks to the laminar flow hood.

[0024] The 2D cultured cells were photographed to confirm that the cell morphology was long spindle-shaped and the confluence was above 80%. The 2D cultured cells were digested, and after digestion, the cells were counted and the cell viability was detected.

[0025] Add 800 mg of microcarriers to each spinner flask, then add 50 mL of 3D cell culture medium to each spinner flask and gently shake the spinner flask to disperse the microcarriers.

[0026] After all the microslides were evenly suspended in the culture medium, 2.0×10 7 cells (i.e. 5×10 5 cells / 20mg microslide).

[0027] Each spinner flask was supplemented with culture medium to 150 mL, and then placed on the bioreactor and set to a rotation speed of 30 rpm for 5 min. The spinner flask was then left to stand for 1 h as one cycle, and a total of 24 cycles were performed.

[0028] 3D culture system observation (first day):

[0029] Move the spinner flask to the 3D FloTrix in the laminar flow hood. ® On the miniSpin bioreactor, the rotation speed was set to 40 rpm. After the microtissue was evenly suspended in the entire culture system, an electric pipette was used to sample from the side arm.

[0030] Take 10 μL of cell culture supernatant, add it dropwise to the glucose test strip, and read the glucose content.

[0031] 100 μL of microtissue suspension was placed in a 96-well plate, the culture supernatant was discarded, 100 μL of live-dead fluorescent staining working solution was added, and incubated at room temperature in the dark for 30 min; the staining solution was discarded and 100 μL of PBS was added, and the plate was observed under a fluorescence microscope and photographed.

[0032] Take three 1 mL microtissue suspensions, discard 900 μL of supernatant, add 900 μL of 1 mg / mL lysis buffer, and place in a 37°C water bath for lysis for 30 min; mix the cell suspension, mix the cell suspension with 0.4% trypan blue dye in a 1:1 ratio, take 20 μL, add it to a cell counting plate, and count the cells.

[0033] Then, 350 mL of 3D cell culture medium was added to each spinner flask, and the rotation speed was adjusted to a constant speed of 40 rpm.

[0034] 3D culture system observation (day 4):

[0035] The steps of sample connection, glucose measurement, live cell fluorescence staining, microcarrier lysis and counting, and rehydration were the same as before.

[0036] 3D culture system observation (day 5):

[0037] The steps of sample connection, glucose measurement, live cell fluorescence staining, and microcarrier lysis and counting were the same as before. 3L of cell culture supernatant was harvested and analyzed by 3D FloTRIX ® The vivaEXO exosome harvesting system produces 3D exosomes.

[0038] Example 1

[0039] A temperature-sensitive material for wound repair comprises 60% of a temperature-sensitive repair base material and 40% of a drug component. In the various embodiments of the present application, no detailed description of the drug component is given. All drugs or drug compositions that can be used for wound repair and have an anti-inflammatory effect can be used in the present application. Those skilled in the art can select the drug component for wound anti-inflammatory according to actual needs, which does not affect the implementation of the technical solution of the present application. In this embodiment and the following embodiments, the selection of the drug component is handled accordingly.

[0040] In this embodiment, the temperature-sensitive repair base includes 75% hydroxybutyl chitosan and the remainder 3D exosomes. The 3D exosomes in this embodiment are prepared by using 2D cells in a 3D culture system.

[0041] Example 2

[0042] A thermosensitive material for wound repair comprises 99.8% of a thermosensitive repair base and 0.2% of a drug component. The thermosensitive repair base comprises 90% hydroxybutyl chitosan and the remainder 3D exosomes. The 3D exosomes of this embodiment are prepared from 2D cells via a 3D culture system.

[0043] Example 3

[0044] A thermosensitive material for wound repair comprises 80% of a thermosensitive repair base and 20% of a drug component. The thermosensitive repair base comprises 80% hydroxybutyl chitosan and the remainder 3D exosomes. The 3D exosomes of this embodiment are prepared from 2D cells via a 3D culture system.

[0045] Example 4

[0046] A thermosensitive material for wound repair comprises 90% of a thermosensitive repair base and 10% of a drug component. The thermosensitive repair base comprises 85% hydroxybutyl chitosan and the remainder 3D exosomes. The 3D exosomes of this embodiment are prepared from 2D cells via a 3D culture system.

[0047] Example 5

[0048] A thermosensitive material for wound repair comprises 85% of a thermosensitive repair base and 15% of a drug component. The thermosensitive repair base comprises 90% hydroxybutyl chitosan and hydroxypropyl chitin, and the remainder is 3D exosomes. The weight ratio of hydroxybutyl chitosan to hydroxypropyl chitin is 3:1. The 3D exosomes of this embodiment are prepared by using 2D cells in a 3D culture system.

[0049] Example 6

[0050] A thermosensitive material for wound repair comprises 95% of a thermosensitive repair base and 5% of a drug component. The thermosensitive repair base comprises 88% hydroxypentyl chitosan and the remainder 3D exosomes. The 3D exosomes of this embodiment are prepared by using 2D cells in a 3D culture system.

[0051] Example 7

[0052] A thermosensitive material for wound repair comprises 75% of a thermosensitive repair base and 25% of a drug component. The thermosensitive repair base comprises 82% of hydroxypentyl chitosan and carboxyl chitin, and the remainder is 3D exosomes. The weight ratio of hydroxypentyl chitosan to carboxyl chitin is 1:1. The 3D exosomes of this embodiment are prepared by using 2D cells in a 3D culture system.

[0053] In the above embodiments of the present application, if there are two or more thermosensitive hydrogels in the thermosensitive repair base, the weight ratio of the two is usually between 1:1 and 5:1.

[0054] The temperature-sensitive materials of the above embodiments can all be used for wound repair. Figures 1 to 3 shown.

[0055] The above are only preferred embodiments of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A thermosensitive material for wound repair, characterized in that: It includes a temperature-sensitive repair base material and a drug component for wound anti-inflammation; the weight percentage of the temperature-sensitive repair base material and the drug component is 60%-99.8%: 0.2%-40%, so that the weight percentage composition of the temperature-sensitive material is 100%.

2. The thermosensitive material for wound repair according to claim 1, characterized in that: The weight percentage of the temperature-sensitive repair base material and the drug component is 80%-99%:1%-20%, so that the weight percentage composition of the temperature-sensitive material is 100%.

3. The thermosensitive material for wound repair according to claim 1, characterized in that: The thermosensitive material also includes drugs for increasing the speed of wound repair, which account for 1-10% of the thermosensitive material by weight.

4. The thermosensitive material for wound repair according to claim 1, characterized in that: The thermosensitive repair base includes a thermosensitive hydrogel and 3D exosomes, and the 3D exosomes are prepared by using 2D cells through a 3D culture system.

5. The thermosensitive material for wound repair according to claim 4, characterized in that: The thermosensitive repair base comprises 75%-90% of the thermosensitive hydrogel and the remainder of the 3D exosomes, based on weight percentage.

6. The thermosensitive material for wound repair according to claim 4, characterized in that: The temperature-sensitive hydrogel is one or a combination of α-ketoglutaric acid, carboxyl chitin, hydroxypropyl chitin, hydroxybutyl chitosan, and hydroxypentyl chitosan.

7. An application, characterized in that: Use of the thermosensitive material for wound repair according to any one of claims 1 to 6 in wound repair.

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