Composition with moisturizing, soothing and repairing effects and application thereof

Abstract

The invention relates to a composition with moisturizing, soothing and repairing effects and application thereof. The composition with the moisturizing, soothing and repairing effects comprises a pomelo fruit extract, a capparis spinosa fruit extract and hydroxyphenyl propionamide benzoic acid. Three active components, namely the pomelo fruit extract, the capparis spinosa fruit extract and the hydroxyphenyl propionamide benzoic acid, are creatively combined for use, wherein the pomelo fruit extract can inhibit release of inflammatory factors, reduce ultraviolet-induced DNA damage and inflammatory response, neutralize free radicals and reduce oxidative stress damage; wherein the capparis spinosa fruit extract can quickly moisturize, enhance cell viability and promote enhancement of a skin barrier; wherein hydroxyphenyl propionamide benzoic acid can block symptoms such as skin redness and swelling, pruritus and pain caused by the fact that histamine activates a TRPV1 pathway. The three components are matched with one another, have a synergistic effect in the aspects of moisturizing, relieving the skin and repairing the skin barrier, and have an excellent effect when being applied to cosmetics with related effects.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and relates to a composition with moisturizing, soothing and repairing effects and an application thereof, and in particular to a composition with moisturizing, soothing and repairing effects and an application thereof in cosmetics. Background Art

[0002] Human skin is a vital barrier that prevents the loss of water, electrolytes, and other substances from the body and blocks the intrusion of harmful external substances. The integrity of the skin barrier is crucial for maintaining skin health. The skin barrier protects the body from external environmental aggressors, including harmful microorganisms, chemicals, pollutants, and physical damage (such as ultraviolet radiation). The skin barrier helps maintain skin moisture and prevents excessive water loss, thereby maintaining skin suppleness and elasticity.

[0003] With the combined effects of environmental changes, irregular lifestyles, skin damage from medical aesthetics, and the overuse of skincare products, the incidence of skin sensitivity and irritation is increasing year by year. The main causes of sensitive skin are the following three aspects: impaired skin barrier function, which leads to a decrease in the moisture content of the stratum corneum, an increase in transepidermal water loss (TEWL), and increased permeability of the stratum corneum, making it easier for external substances to enter the skin and cause irritation; an aggravated inflammatory response, excessive penetration of external irritants, and the synthesis and release of large amounts of inflammatory mediators by the skin, triggering a cascade of inflammatory reactions; and highly reactive nerve endings. After inflammation and tissue damage, inflammatory agents are released, activating primary sensory neurons to release peptides and neurotransmitters, making them more receptive to pain signals mediated by inflammatory factors.

[0004] Given the prevalence of sensitive skin among modern people, moisturizing, soothing, and repairing ingredients are essential in skincare products. They replenish moisture and nutrients, restore skin's health and vitality, enhance skin comfort and aesthetics, and satisfy people's pursuit of healthy skin. However, existing products with moisturizing, soothing, and repairing properties often have limited efficacy through a single pathway. Therefore, it is crucial to develop skincare products that are suitable for sensitive skin, offering excellent moisturizing, soothing, and repairing effects, while offering multiple pathways to inhibit sensitivity. Summary of the Invention

[0005] In view of the deficiencies of the prior art, the object of the present invention is to provide a composition with moisturizing, soothing and repairing effects and its application, in particular to provide a composition with moisturizing, soothing and repairing effects and its application in cosmetics.

[0006] In order to achieve the purpose of the invention, the present invention adopts the following technical solutions:

[0007] In a first aspect, the present invention provides a composition with moisturizing, soothing and repairing effects, wherein the composition with moisturizing, soothing and repairing effects comprises pomelo fruit extract, caper fruit extract and hydroxyphenylpropionamide benzoic acid.

[0008] The present invention creatively combines three active ingredients: pomelo fruit extract, caper fruit extract, and hydroxyphenylpropionamide benzoic acid. The pomelo fruit extract can inhibit the release of inflammatory factors, regulate inflammatory pathways such as NF-κB, and alleviate inflammatory problems such as skin sensitivity, redness, and acne; it can also reduce UV-induced DNA damage and inflammatory reactions; and it can also neutralize free radicals and reduce oxidative stress damage. The caper fruit extract can effectively increase Capspase14 activity in the short term, accelerate the degradation of filaggrin into natural moisturizing factor, and quickly moisturize; and can inhibit Capspase14 release in the long term, effectively increasing filaggrin reserves, enhancing cell viability, and promoting the strengthening of the skin barrier. Hydroxyphenylpropionamide benzoic acid, also known as dihydroavenous alkaloid D, is an active ingredient derived from oats. Its structure is similar to that of the antihistamine drug trenilast. It can competitively block the binding of histamine to the histamine H1 receptor, thereby blocking the skin redness, itching, pain, and other symptoms caused by histamine activating the TRPV1 pathway. These three components work together to synergistically enhance moisturizing, soothing the skin, and repairing the skin barrier, and have excellent effects when applied to cosmetics with related functions.

[0009] Preferably, the composition with moisturizing, soothing and repairing effects comprises, by weight, 1-5 parts of pomelo fruit extract, 2-10 parts of caper fruit extract and 1-5 parts of hydroxyphenylpropionamide benzoic acid.

[0010] Based on the potential synergistic effects of the three active ingredients, pomelo fruit extract, caper fruit extract and hydroxyphenylpropionamide benzoic acid, in moisturizing, soothing the skin and repairing the skin barrier, the effect will be better when they meet the above-mentioned specific mass ratio.

[0011] The mass fraction of the pomelo fruit extract can be selected from 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, etc.; the mass fraction of the caper fruit extract can be selected from 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, etc.; the mass fraction of the hydroxyphenylpropionamide benzoic acid can be selected from 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, etc.; other specific point values ​​within the above numerical ranges can be selected, and they will not be repeated here.

[0012] The pomelo fruit extract involved in the present invention is not limited to its specific preparation process. When it is prepared using the following process (i.e., the pomelo fruit raw material is subjected to water extraction, enzymatic hydrolysis and alcohol extraction in sequence), its synergistic effect with the caper fruit extract and hydroxyphenylpropionamide benzoic acid in moisturizing, soothing the skin and repairing the skin barrier is more obvious.

[0013] Preferably, the pomelo fruit extract is prepared by a preparation method comprising the following steps:

[0014] (1) mixing a pomelo fruit raw material with water and performing an extraction process, separating the extract into solid and liquid, and concentrating and drying the supernatant to obtain a pomelo fruit water extract product;

[0015] (2) mixing the pomelo fruit water extract, enzyme, and water for enzymatic hydrolysis, separating the enzymatic hydrolyzate into solid and liquid, and concentrating and drying the supernatant to obtain a pomelo fruit enzymatic hydrolyzate;

[0016] (3) The pomelo fruit enzymatic hydrolysis product is mixed with an ethanol aqueous solution and then subjected to extraction treatment, the extract is separated into solid and liquid, and the supernatant is concentrated and dried to obtain the pomelo fruit extract.

[0017] The pomelo fruit raw material can be either a dried pomelo fruit raw material or a non-dried pomelo fruit raw material, and can be either a crushed state or a non-crushed state.

[0018] Preferably, the mass ratio of the pomelo fruit raw material to water in step (1) is 1:(5-50), for example, 1:5, 1:8, 1:10, 1:15, 1:20, 1:23, 1:25, 1:28, 1:30, 1:35, 1:37, 1:40, 1:45, 1:50, etc.

[0019] Preferably, the extraction treatment in step (1) is carried out at 70-90°C (e.g., 70°C, 73°C, 75°C, 78°C, 80°C, 82°C, 85°C, 90°C, etc.) for 1-5h (e.g., 1h, 1.5h, 2h, 2.5h, 3h, 3.5h, 4h, 4.5h, 5h, etc.).

[0020] Preferably, the enzyme in step (2) is a combination of cellulase, xylanase and pectinase.

[0021] In the enzymatic hydrolysis process of preparing pomelo fruit extract, the combined use of cellulase, xylanase and pectinase can achieve better extraction results, and the three enzymes synergistically enhance the moisturizing, soothing and repairing effects of the product.

[0022] Preferably, the mass ratio of the cellulase, xylanase and pectinase is (4-7):(2-3):1, for example, 4:2:1, 5:2:1, 6:2:1, 7:2:1, 4:3:1, 5:3:1, 6:3:1, 7:3:1, etc.

[0023] Preferably, the added amount of the enzyme is 0.1‰-1‰ of the mass of the pomelo fruit raw material, for example, 0.1‰, 0.2‰, 0.3‰, 0.4‰, 0.5‰, 0.6‰, 0.7‰, 0.8‰, 0.9‰, 1‰, etc.

[0024] Preferably, the mass ratio of the pomelo fruit water extract product to water in step (2) is 1:(5-15), for example, 1:5, 1:6, 1:7, 1:8, 1:9, 1:10, 1:11, 1:12, 1:13, 1:14, 1:15, etc.

[0025] Preferably, the enzymatic hydrolysis treatment in step (2) is carried out at 35-50°C (e.g., 35°C, 38°C, 40°C, 42°C, 45°C, 47°C, 48°C, 50°C, etc.) for 1-3h (e.g., 1h, 1.5h, 2h, 2.5h, 3h, etc.).

[0026] Preferably, the ethanol concentration of the ethanol aqueous solution in step (3) is 50-80%, such as 50%, 55%, 60%, 65%, 70%, 75%, 80%, etc.

[0027] Preferably, the mass ratio of the pomelo fruit enzymatic hydrolysate to the ethanol aqueous solution in step (3) is 1:(10-20), for example, 1:10, 1:11, 1:12, 1:13, 1:14, 1:15, 1:16, 1:17, 1:18, 1:19, 1:20, etc.

[0028] Preferably, the extraction treatment in step (3) adopts reflux extraction for 1-3 hours, such as 1 hour, 1.5 hours, 2 hours, 2.5 hours, 3 hours, etc.

[0029] Other specific point values ​​within the above numerical ranges can be selected and will not be described in detail here.

[0030] The caper fruit extract involved in the present invention is not limited to its specific preparation process. When it is prepared using the following process (i.e., ultrasound-assisted enzymatic hydrolysis treatment), its synergistic effect with pomelo fruit extract and hydroxyphenylpropionamide benzoic acid in moisturizing, soothing the skin, and repairing the skin barrier is more obvious.

[0031] Preferably, the Capparis spinosa fruit extract is prepared by a preparation method comprising the following steps:

[0032] The caper fruit raw material, enzyme and water are mixed, ultrasound-assisted enzymolysis is performed, the enzymolysis liquid is separated into solid and liquid, and the clear liquid is concentrated and dried to obtain the caper fruit extract.

[0033] The caper fruit raw material can be either a dried caper fruit raw material or a non-dried raw material, and can be either in a crushed state or a non-crushed state.

[0034] Preferably, the enzyme is a combination of cellulase, macease and papain.

[0035] Preferably, the mass ratio of the cellulase, macerate and papain is (1-5):(1-5):(0.1-2).

[0036] The specific point values ​​in (1-5) can be 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, etc.; the specific point values ​​in (0.1-2) can be 0.1, 0.2, 0.4, 0.5, 0.6, 0.8, 1, 1.2, 1.4, 1.5, 1.6, 1.8, 2, etc.

[0037] Preferably, the added amount of the enzyme is 0.1‰-1‰ of the mass of the caper fruit raw material, for example, 0.1‰, 0.2‰, 0.3‰, 0.4‰, 0.5‰, 0.6‰, 0.7‰, 0.8‰, 0.9‰, 1‰, etc.

[0038] Preferably, the mass ratio of the caper fruit raw material to water is 1:(5-40), for example, 1:5, 1:8, 1:10, 1:15, 1:18, 1:20, 1:22, 1:25, 1:30, 1:35, 1:40, etc.

[0039] Preferably, the enzymatic hydrolysis treatment is carried out at 45-55°C (eg, 45°C, 47°C, 48°C, 50°C, 55°C, etc.) for 1-3h (eg, 1h, 1.5h, 2h, 2.5h, 3h, etc.).

[0040] Preferably, the power of the ultrasound is 150-450W, for example, 150W, 180W, 200W, 220W, 240W, 260W, 280W, 300W, 320W, 350W, 380W, 400W, 450W, etc.

[0041] Other specific point values ​​within the above numerical ranges can be selected and will not be described in detail here.

[0042] In a second aspect, the present invention provides use of the composition with moisturizing, soothing and repairing effects according to the first aspect in the preparation of cosmetics.

[0043] Preferably, the cosmetics include lotions, emulsions, creams, essences, facial masks, sunscreen products, makeup removers, and cleansing products.

[0044] In a third aspect, the present invention provides a cosmetic having moisturizing, soothing and repairing effects, the cosmetic comprising the composition of the first aspect, a polyol, an emollient, an emulsifier, a skin conditioner, a thickener, an antioxidant and a solvent.

[0045] Preferably, the skin conditioning agent comprises a combination of rice bran oil and shea butter.

[0046] The present invention also creatively discovered that adding rice bran oil and avocado butter to cosmetics containing the aforementioned composition can further enhance the product's moisturizing, skin soothing, and skin barrier repairing effects, and that rice bran oil and avocado butter synergistically enhance the above-mentioned enhanced effects.

[0047] Preferably, the polyol includes any one of glycerol, butanediol, 1,2-hexanediol or 1,2-pentanediol, or a combination of at least two thereof.

[0048] Preferably, the emollient comprises any one of behenyl alcohol, pentaerythritol tetra(ethylhexanoate), cetearyl alcohol or polydimethylsiloxane, or a combination of at least two thereof.

[0049] Preferably, the emulsifier includes any one of cetearyl olivate, sorbitan olivate, cetearyl glucoside or cetearyl alcohol, or a combination of at least two thereof.

[0050] Preferably, the thickener comprises carbomer and / or ammonium acryloyldimethyltaurate / VP copolymer.

[0051] Preferably, the antioxidant comprises p-hydroxyacetophenone.

[0052] Preferably, the cosmetic further comprises a pH adjuster and / or a preservative.

[0053] Compared with the prior art, the present invention has the following beneficial effects:

[0054] This invention creatively combines three active ingredients: pomelo extract, caper fruit extract, and hydroxyphenylpropionamide benzoic acid. The pomelo extract inhibits the release of inflammatory factors, reduces UV-induced DNA damage and inflammatory responses, neutralizes free radicals, and reduces oxidative stress. The caper fruit extract rapidly moisturizes, enhances cell viability, and promotes skin barrier strengthening. The hydroxyphenylpropionamide benzoic acid blocks histamine-activated TRPV1 pathway, which can cause skin redness, itching, and pain. These three ingredients work together to synergistically enhance moisturizing, soothing, and skin barrier repair, resulting in excellent results when applied to cosmetics with these benefits. DETAILED DESCRIPTION

[0055] In order to further illustrate the technical means and effects adopted by the present invention, the technical solutions of the present invention are further described below in conjunction with the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.

[0056] The sources of the effective ingredients contained in the products involved in the following embodiments, comparative examples, application examples, and comparative application examples are as follows (only the effective ingredients are reflected, and other necessary auxiliary ingredients contained in commercially available raw materials are not repeated).

[0057]

[0058]

[0059] Preparation Example 1-1

[0060] This preparation example provides a pomelo fruit extract, which is prepared by the following method:

[0061] (1) The pomelo fruit was dried and crushed, mixed with 15 times the amount of water, and subjected to extraction treatment at 80°C for 3 hours. The extract was filtered, and the filtrate was concentrated and dried to obtain a pomelo fruit water extract product;

[0062] (2) The pomelo fruit water extract, enzyme, and water were mixed and subjected to enzymatic hydrolysis at 40°C for 2 h. The enzymatic hydrolyzate was filtered, and the filtrate was concentrated and dried to obtain a pomelo fruit enzymatic hydrolyzate; wherein the enzyme was a combination of cellulase, xylanase, and pectinase in a mass ratio of 5:2:1; the amount of enzyme added was 0.5‰ of the pomelo fruit water extract; and the amount of water used was 10 times the mass of the pomelo fruit water extract;

[0063] (3) The pomelo fruit enzymatic hydrolysate was mixed with 15 times the mass of 65% ethanol aqueous solution and subjected to reflux extraction for 2 hours. The extract was filtered, and the filtrate was concentrated and dried to obtain the pomelo fruit extract.

[0064] Preparation Example 1-2

[0065] This preparation example provides a pomelo fruit extract, which is prepared by the following method:

[0066] (1) The pomelo fruit was dried and crushed, mixed with 20 times water, and subjected to extraction treatment at 85°C for 2.5 hours. The extract was filtered, and the filtrate was concentrated and dried to obtain a pomelo fruit water extract product;

[0067] (2) The pomelo fruit water extract, enzyme, and water were mixed and subjected to enzymatic hydrolysis at 35°C for 3 h. The enzymatic hydrolyzate was filtered, and the filtrate was concentrated and dried to obtain a pomelo fruit enzymatic hydrolyzate; wherein the enzyme was a combination of cellulase, xylanase, and pectinase in a mass ratio of 6:3:1; the amount of enzyme added was 0.5‰ of the pomelo fruit water extract; and the amount of water used was 12 times the mass of the pomelo fruit water extract;

[0068] (3) The pomelo fruit enzymatic hydrolysate was mixed with 10 times the mass of 75% ethanol aqueous solution and subjected to reflux extraction for 1.5 hours. The extract was filtered, and the filtrate was concentrated and dried to obtain the pomelo fruit extract.

[0069] Preparation Examples 1-3

[0070] This preparation example provides a pomelo fruit extract, which is prepared by the following method:

[0071] (1) The pomelo fruit was dried and crushed, mixed with 10 times the amount of water, and subjected to extraction treatment at 75°C for 4 hours. The extract was filtered, and the filtrate was concentrated and dried to obtain a pomelo fruit water extract product;

[0072] (2) The pomelo fruit water extract, enzyme, and water were mixed and subjected to enzymatic hydrolysis at 45°C for 1.5 h. The enzymatic hydrolyzate was filtered, and the filtrate was concentrated and dried to obtain a pomelo fruit enzymatic hydrolyzate; wherein the enzyme was a combination of cellulase, xylanase, and pectinase in a mass ratio of 7:3:1; the amount of enzyme added was 0.5‰ of the pomelo fruit water extract; and the amount of water used was 12 times the mass of the pomelo fruit water extract;

[0073] (3) The pomelo fruit enzymatic hydrolysate was mixed with 10 times the mass of 60% ethanol aqueous solution and subjected to reflux extraction for 2.5 hours. The extract was filtered, and the filtrate was concentrated and dried to obtain the pomelo fruit extract.

[0074] Preparation Examples 1-4

[0075] This preparation example provides a pomelo fruit extract, the preparation method of which differs from that of Preparation Example 1-1 only in that the hydrolysis treatment in step (1) is missing:

[0076] (1) The dried and crushed pomelo fruit, enzyme, and water were mixed and subjected to enzymatic hydrolysis at 40°C for 2 h. The enzymatic hydrolyzate was filtered, and the filtrate was concentrated and dried to obtain a pomelo fruit enzymatic hydrolyzate; wherein the enzyme was a combination of cellulase, xylanase, and pectinase in a mass ratio of 5:2:1; the amount of enzyme added was 0.8‰ of the dried and crushed pomelo fruit; and the amount of water used was 10 times the mass of the dried and crushed pomelo fruit;

[0077] (2) The pomelo fruit enzymatic hydrolysate was mixed with 15 times the mass of a 65% ethanol aqueous solution and subjected to reflux extraction for 2 hours. The extract was filtered, and the filtrate was concentrated and dried to obtain the pomelo fruit extract.

[0078] Preparation Examples 1-5

[0079] This preparation example provides a pomelo fruit extract, the preparation method of which differs from that of Preparation Example 1-1 only in that the alcohol extraction treatment in step (3) is missing:

[0080] (1) The pomelo fruit was dried and crushed, mixed with 15 times the amount of water, and subjected to extraction treatment at 80°C for 3 hours. The extract was filtered, and the filtrate was concentrated and dried to obtain a pomelo fruit water extract product;

[0081] (2) The pomelo fruit water extract, enzyme and water are mixed, and enzymatic hydrolysis is performed at 40°C for 2 hours. The enzymatic hydrolysis solution is filtered, and the filtrate is concentrated and dried to obtain the pomelo fruit extract; wherein the enzyme is a combination of cellulase, xylanase and pectinase in a mass ratio of 5:2:1; the amount of enzyme added is 0.5‰ of the pomelo fruit water extract; and the amount of water used is 10 times the mass of the pomelo fruit water extract.

[0082] Preparation Examples 1-6

[0083] This preparation example provides a pomelo fruit extract, the preparation method of which differs from that of Preparation Example 1-1 only in that the enzyme in step (2) is a combination of cellulase and xylanase in a mass ratio of 5:2, and the total amount of enzymes and other conditions remain unchanged.

[0084] Preparation Examples 1-7

[0085] This preparation example provides a pomelo fruit extract, the preparation method of which differs from that of Preparation Example 1-1 only in that the enzyme in step (2) is a combination of cellulase and pectinase in a mass ratio of 5:1, and the total amount of enzymes and other conditions remain unchanged.

[0086] Preparation Examples 1-8

[0087] This preparation example provides a pomelo fruit extract, the preparation method of which differs from that of Preparation Example 1-1 only in that the enzyme in step (2) is a combination of xylanase and pectinase in a mass ratio of 2:1, and the total amount of enzymes and other conditions remain unchanged.

[0088] Preparation Example 2-1

[0089] This preparation example provides a Capparis spinosa fruit extract, which is prepared by the following method:

[0090] (1) The caper fruit was dried and crushed, mixed with enzyme and water, and subjected to ultrasonic-assisted enzymatic hydrolysis at 50°C for 1.5 hours. The enzymatic hydrolyzate was filtered and the filtrate was collected; wherein the enzyme was a combination of cellulase, cleavage enzyme and papain in a mass ratio of 4:3:1; the amount of enzyme added was 0.5‰ of the caper fruit raw material; the amount of water used was 20 times the mass of the caper fruit raw material; wherein the power of the ultrasound was 300W;

[0091] (2) Repeat the above steps once with the filter residue obtained in step (1), collect the combined filtrate, concentrate and dry to obtain the capparis spinosa fruit extract.

[0092] Preparation Example 2-2

[0093] This preparation example provides a Capparis spinosa fruit extract, which is prepared by the following method:

[0094] (1) The caper fruit was dried and crushed, then mixed with enzyme and water, and subjected to ultrasonic-assisted enzymatic hydrolysis at 45°C for 1.5 hours. The enzymatic hydrolyzate was filtered and the filtrate was collected; wherein the enzyme was a combination of cellulase, cleavage enzyme and papain in a mass ratio of 3:2:2; the amount of enzyme added was 0.5‰ of the caper fruit raw material; the amount of water used was 15 times the mass of the caper fruit raw material; wherein the power of the ultrasound was 400W;

[0095] (2) Repeat the above steps once with the filter residue obtained in step (1), collect the combined filtrate, concentrate and dry to obtain the capparis spinosa fruit extract.

[0096] Preparation Example 2-3

[0097] This preparation example provides a Capparis spinosa fruit extract, which is prepared by the following method:

[0098] (1) The caper fruit was dried and crushed, then mixed with enzyme and water, and subjected to ultrasonic-assisted enzymatic hydrolysis at 55°C for 1 hour. The enzymatic hydrolyzate was filtered and the filtrate was collected; wherein the enzyme was a combination of cellulase, cleavage enzyme and papain in a mass ratio of 2:2:0.5; the amount of enzyme added was 0.5‰ of the caper fruit raw material; the amount of water used was 15 times the mass of the caper fruit raw material; wherein the power of the ultrasound was 250W;

[0099] (2) Repeat the above steps once with the filter residue obtained in step (1), collect the combined filtrate, concentrate and dry to obtain the capparis spinosa fruit extract.

[0100] Example 1

[0101] This embodiment provides a composition with moisturizing, soothing and repairing effects, which is composed of 3 parts of pomelo fruit extract (Preparation Example 1-1), 5 parts of caper fruit extract (Preparation Example 2-1) and 2 parts of hydroxyphenylpropionamide benzoic acid, in parts by mass.

[0102] Example 2

[0103] This embodiment provides a composition with moisturizing, soothing and repairing effects, which is composed of 5 parts of pomelo fruit extract (Preparation Example 1-2), 2 parts of caper fruit extract (Preparation Example 2-2) and 1 part of hydroxyphenylpropionamide benzoic acid, in parts by mass.

[0104] Example 3

[0105] This embodiment provides a composition with moisturizing, soothing and repairing effects, which is composed of 2 parts of pomelo fruit extract (Preparation Example 1-3), 8 parts of caper fruit extract (Preparation Example 2-3) and 5 parts of hydroxyphenylpropionamide benzoic acid, in parts by mass.

[0106] Example 4

[0107] This embodiment provides a composition with moisturizing, soothing and repairing effects, which is composed, by mass, of 3 parts of pomelo fruit extract (commercially available), 5 parts of caper fruit extract (commercially available) and 2 parts of hydroxyphenylpropionamide benzoic acid.

[0108] Examples 5-9

[0109] Five compositions with moisturizing, soothing and repairing effects are provided. The components thereof differ from those of Example 1 only in that the pomelo fruit extract of Preparation Example 1-1 is replaced by the pomelo fruit extracts of Preparation Examples 1-4 to 1-8 in equal parts by weight, and the other components and parts by weight remain unchanged.

[0110] Comparative Example 1

[0111] This comparative example provides a composition, the components of which differ from those of Example 1 only in that the pomelo fruit extract is missing, and the reduced mass fraction thereof is proportionally distributed to the mass fractions of the capparis spinosa fruit extract and hydroxyphenylpropionamidobenzoic acid.

[0112] Comparative Example 2

[0113] This comparative example provides a composition, the components of which differ from those of Example 1 only in that the Capparis spinosa fruit extract is missing, and the reduced mass fraction thereof is proportionally distributed to the mass fractions of the pomelo fruit extract and hydroxyphenylpropionamide benzoic acid.

[0114] Comparative Example 3

[0115] This comparative example provides a composition, the components of which differ from those of Example 1 only in the lack of hydroxyphenylpropionamide benzoic acid, and the reduced mass fraction thereof is proportionally distributed to the mass fractions of pomelo fruit extract and capparis spinosa fruit extract.

[0116] Application Example 1

[0117] This application example provides a cosmetic with moisturizing, soothing and repairing effects, the formula of which is as follows:

[0118]

[0119] The preparation method is as follows:

[0120] (1) Put phase A into oil pan at 80℃ and dissolve and mix evenly; dissolve phase B at 80℃ and mix evenly; dissolve phase C and phase D at 60℃ in advance and cool to room temperature for later use;

[0121] (2) Add phase A to phase B while stirring and homogenize at 3000 rpm for 5 min;

[0122] (3) Lower the temperature to 40°C, add phases C and D in sequence, and stir evenly until the temperature reaches room temperature.

[0123] Application Example 2-9

[0124] This application example provides eight cosmetics, the formulas of which differ from those of Application Example 1 only in that the composition prepared in Example 1 is replaced with the compositions prepared in Examples 2-9 in equal amounts, and the other ingredients and contents remain unchanged. The preparation method refers to that of Application Example 1.

[0125] Application Example 10

[0126] This application example provides a cosmetic, the formula of which differs from that of Application Example 1 only in that it does not contain rice bran oil, and a reduced amount of it is distributed to avocado tree butter. Other ingredients and contents remain unchanged. The preparation method refers to Application Example 1.

[0127] Application Example 11

[0128] This application example provides a cosmetic, the formula of which differs from that of Application Example 1 only in that it does not contain avocado tree butter, and its reduced amount is distributed to rice bran oil. Other ingredients and contents remain unchanged. The preparation method refers to Application Example 1.

[0129] Comparative Application Examples 1-3

[0130] This comparative application example provides three cosmetics, the formulas of which differ from those of Application Example 1 only in that the composition prepared in Example 1 is replaced with the compositions prepared in Comparative Examples 1-3 in equal amounts, and the other ingredients and contents remain unchanged. The preparation method refers to that of Application Example 1.

[0131] Test Example 1

[0132] Soothing effect evaluation:

[0133] (1) Preparation of test samples: The products prepared in Examples 1-9 and Comparative Examples 1-3 were prepared into test samples with a concentration of 1% for later use.

[0134] (2) Experimental procedure: Test tubes A and B (0.5 mL of sample solution and 0.5 mL of hyaluronidase (500 U / mL)), test tubes C and D (0.5 mL of distilled water and 0.5 mL of acetic acid buffer solution), incubated at 37°C for 20 min; 0.1 mL (2.5 mmol / L) of CaCl2 solution was added, and incubated at 37°C for 20 min; 0.5 mL of sodium hyaluronate (0.5 mg / mL) was added to test tubes A and C, and 0.5 mL of acetic acid buffer solution was added to test tubes B and D, and incubated at 37°C for 40 min, and 0.5 mL of acetylacetone solution and 0.1 mL of acetic acid buffer solution were added. NaOH solution (5 mol / L) and 0.5 mL of distilled water were added and boiled in a water bath for 15 min. Immediately cooled in ice water for 5 min, 1 mL of P-DAB colorimetric reagent was added, and after sufficient shaking, 3.5 mL of anhydrous ethanol was added. The mixture was allowed to stand for 30 min for color development, and the OD values ​​of the four test tubes A, B, C, and D were measured at 530 nm, respectively.

[0135] The calculation formula for the hyaluronidase activity inhibition rate is:

[0136] Hyaluronidase inhibition rate (%) = [(CD) - (AB)] / (CD) × 100

[0137] (3) The results are shown in Table 1:

[0138] Table 1

[0139]

[0140]

[0141] As shown in Table 1, compared to the products of Comparative Examples 1-3, the present invention utilizes a combination of pomelo fruit extract, capparis spinosa fruit extract, and hydroxyphenylpropionamide benzoic acid, resulting in a synergistic effect in enhancing the soothing efficacy of the product, resulting in superior results. Furthermore, a comparison of the results of Example 1 with those of Examples 4-9 shows that the preparation processes of the pomelo fruit extract and capparis spinosa fruit extract also affect the soothing efficacy of the product to a certain extent.

[0142] Test Example 2

[0143] Evaluation of inflammatory factor inhibition:

[0144] This test example uses an LPS-induced mouse macrophage (RAW264.7) inflammation model to determine the inhibitory effect of the sample on tumor necrosis factor-α (TNF-α).

[0145] (1) The MTT method was used to determine the effect of sample solutions obtained by diluting the product of Example 1 at different concentrations on the cell viability of mouse macrophages (RAW264.7). According to the test results, when the sample solution concentration was ≤1.5%, the cell viability was greater than 95%. Therefore, 1.00% was selected as a safe concentration for subsequent experiments.

[0146] (2) Preparation of test samples: The products prepared in Examples 1-9 and Comparative Examples 1-3 were prepared into test samples with a concentration of 1% for later use.

[0147] (3) Adjust the density of RAW264.7 cells to 5×10 4 Cells were seeded into 96-well plates at a volume of 200 μL per well and placed back into the incubator for incubation (37° C., 5% CO 2 ).

[0148] (4) Grouping: blank control group (no LPS stimulation and no sample intervention, given 180 μL of basal culture medium), negative control group (1 μg / mL LPS stimulation but no sample intervention), experimental groups 1-12 (1 μg / mL LPS stimulation and 180 μL of 1% sample solution of Examples 1-9 and Comparative Examples 1-3 intervention), and 3 cell-free wells as zeroing groups, and plate layout according to 3 replicate wells per group.

[0149] (5) Each group was first added with basal culture medium or sample solution, and then returned to the incubator for incubation (37°C, 5% CO2); after 1 hour, the 96-well plate was removed, 20 μL of basal culture medium was added to each well of the zero well and blank control group, and 20 μL of LPS (1 μg / mL) was added to each well of the negative control group and experimental groups 1-12, and then returned to the incubator for incubation.

[0150] (6) After 24 h, the 96-well plate was removed and the cell supernatant corresponding to each sample group was collected into a centrifuge tube. The supernatant was centrifuged at 1000 rpm for 10 min. The supernatant was collected and placed into a 1.5 mL centrifuge tube. The TNF-α concentration in the cell supernatant was determined using an ELISA kit. The results are shown in Table 2.

[0151] Table 2

[0152]

[0153]

[0154] As shown in Table 2, compared to the products of Comparative Examples 1-3, the present invention utilizes a combination of pomelo fruit extract, capparis spinosa fruit extract, and hydroxyphenylpropionamide benzoic acid, resulting in a synergistic effect in enhancing the anti-inflammatory efficacy of the product, resulting in superior results. Furthermore, a comparison of the results of Example 1 with those of Examples 4-9 shows that the preparation processes of the pomelo fruit extract and capparis spinosa fruit extract also affect the anti-inflammatory efficacy of the product to a certain extent.

[0155] Test Example 3

[0156] Moisturizing effect evaluation:

[0157] (1) Preparation of test samples: The products prepared in Application Examples 1-11 and Comparative Application Examples 1-3 are used as test samples.

[0158] (2) Volunteers (140 people, 14 groups, 10 people each) did not use any products (cosmetics or topical medications) on the test area for 7 days before the test and did not come into contact with water for 3 hours. They cleaned the inner forearms of both hands and then balanced in the test environment for 30 minutes.

[0159] (3) Inject 5% sodium lauryl sulfate solution into the patch test chamber to soak the filter paper; apply the patch tester after administration to the curved side of the left and right forearms of the subject, 3 on each side, with the distance between the edges of adjacent patch test chambers not less than 1 cm, and press lightly with the palm of your hand to evenly apply it on the skin for 24 hours. After removing the patch tester, the subject balances in the test environment for 30 minutes, and uses a stratum corneum hydration meter to measure the water content of the center of each patch area. Each area is measured three times in parallel, and the measurement results are expressed as the average value of the patch area detection value as the initial value. After the initial value measurement is completed, take each group of samples (5mg / cm 2 ) were applied to the test area. One hour after application of the sample, the test area was measured using a stratum corneum hydration meter, and the change rate (%) of the water content of the skin stratum corneum of each group was calculated, as shown in Table 3.

[0160] Table 3

[0161]

[0162] As shown in Table 3, compared to the products in Comparative Application Examples 1-3, the present invention utilizes a combination of pomelo fruit extract, caper fruit extract, and hydroxyphenylpropionamide benzoic acid, resulting in a synergistic effect in enhancing the moisturizing and repairing efficacy of the product, resulting in superior results. Further, by comparing the results of Application Example 1 with those of Application Examples 4-11, it can be seen that the preparation processes of the pomelo fruit extract and caper fruit extract, as well as the choice of skin conditioning agent, also affect the moisturizing and repairing efficacy of the product to a certain extent.

[0163] Test Example 4

[0164] Barrier repair efficacy evaluation:

[0165] (1) Preparation of test samples: The products prepared in Application Example 1, Application Examples 10-11, and Comparative Application Examples 1-3 are prepared as test samples.

[0166] (2) Volunteers (70 people, 7 groups, 10 people in each group, with skin conditions of redness due to allergies, sunburn or inflammation, aged 20-40 years old), of which Group 1 was the control group and did not use the functional product; Groups 2-7 tried the products prepared in Application Example 1, Application Examples 10-11 and Comparative Application Examples 1-3, respectively. Each person used the same amount of product once a day, morning and evening. The transepidermal water loss value (skin water loss tester) and skin redness value (skin color test probe) were tested before use and after 6 weeks of use. The test results are shown in Table 4.

[0167] Table 4

[0168] Group Transepidermal water loss reduction Skin redness reduction Application Example 1 9.56 1.42 Application Example 10 9.14 1.14 Application Example 11 9.63 1.38 Comparative Application Example 1 6.84 0.87 Comparative Application Example 2 7.24 0.93 Comparative Application Example 3 7.55 0.72 control group 0.38 0.15

[0169] As shown in Table 4, compared to the products of Comparative Application Examples 1-3, the present invention, by combining pomelo fruit extract, caper fruit extract, and hydroxyphenylpropionamide benzoic acid, achieves a synergistic effect in enhancing the skin barrier repair efficacy of the product, resulting in superior results. Further, by comparing the results of Application Example 1 with those of Application Examples 10-11, it can be seen that the addition of rice bran oil and avocado butter to cosmetics containing the aforementioned composition can further enhance the product's skin soothing and skin barrier repair efficacy, and that rice bran oil and avocado butter synergize in these enhanced efficacy.

[0170] Test Example 5

[0171] Safety evaluation:

[0172] The volunteers were the same as those in Test Example 3.

[0173] Place each product group into a patch test chamber, leaving the control well untreated. Apply the patch test chamber containing the test substance to the inner forearm of the subject using hypoallergenic tape. Gently press the test chamber with the palm of your hand to evenly adhere the test substance to the skin for 24 hours. Observe the skin for any reactions according to the criteria listed in the table below, 30 minutes and 24 hours after removing the test substance patch.

[0174]

[0175] The results showed that the products of Application Examples 1-11 of the present invention and Comparative Application Examples 1-3 all had negative reactions after trial use, indicating that the products of the present invention are safe and mild and will not cause adverse reactions such as skin irritation and sensitization.

[0176] The applicant declares that the present invention is illustrated by the above-described embodiments, but the present invention is not limited to the above-described embodiments. This does not mean that the present invention must rely on the above-described embodiments in order to be implemented. Those skilled in the art should understand that any improvements to the present invention, equivalent replacements for raw materials in the present invention, additions of auxiliary ingredients, and selection of specific methods, etc., fall within the scope of protection and disclosure of the present invention.

[0177] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, various simple modifications can be made to the technical solution of the present invention, and these simple modifications all fall within the scope of protection of the present invention.

[0178] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any appropriate manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

Claims

1. A composition with moisturizing, soothing and repairing effects, characterized in that: The composition with moisturizing, soothing and repairing effects comprises pomelo fruit extract, caper fruit extract and hydroxyphenylpropionamide benzoic acid.

2. The composition with moisturizing, soothing and repairing effects according to claim 1, characterized in that: The composition with moisturizing, soothing and repairing effects comprises, by weight, 1-5 parts of pomelo fruit extract, 2-10 parts of caper fruit extract and 1-5 parts of hydroxyphenylpropionamide benzoic acid.

3. The composition with moisturizing, soothing and repairing effects according to claim 1 or 2, characterized in that: The pomelo fruit extract is prepared by a preparation method comprising the following steps: (1) mixing a pomelo fruit raw material with water and performing an extraction process, separating the extract into solid and liquid, and concentrating and drying the supernatant to obtain a pomelo fruit water extract product; (2) mixing the pomelo fruit water extract, enzyme, and water for enzymatic hydrolysis, separating the enzymatic hydrolyzate into solid and liquid, and concentrating and drying the supernatant to obtain a pomelo fruit enzymatic hydrolyzate; (3) The pomelo fruit enzymatic hydrolysis product is mixed with an ethanol aqueous solution and then subjected to extraction treatment, the extract is separated into solid and liquid, and the supernatant is concentrated and dried to obtain the pomelo fruit extract.

4. The composition with moisturizing, soothing and repairing effects according to claim 3, characterized in that: The mass ratio of the pomelo fruit raw material to water in step (1) is 1:(5-50); Preferably, the extraction treatment in step (1) is carried out at 70-90° C. for 1-5 hours; Preferably, the enzyme in step (2) is a combination of cellulase, xylanase and pectinase; Preferably, the mass ratio of the cellulase, xylanase and pectinase is (4-7):(2-3):1; Preferably, the added amount of the enzyme is 0.1‰-1‰ of the mass of the pomelo fruit raw material; Preferably, the mass ratio of the pomelo fruit water extract to water in step (2) is 1:(5-15); Preferably, the enzymatic hydrolysis treatment in step (2) is carried out at 35-50° C. for 1-3 h; Preferably, the ethanol concentration of the ethanol aqueous solution in step (3) is 50-80%; Preferably, the mass ratio of the pomelo fruit enzymatic hydrolysate to the ethanol aqueous solution in step (3) is 1:(10-20); Preferably, the extraction treatment in step (3) adopts reflux extraction for 1-3 hours.

5. The composition with moisturizing, soothing and repairing effects according to any one of claims 1 to 4, characterized in that: The capparis spinosa fruit extract is prepared by a preparation method comprising the following steps: The caper fruit raw material, enzyme and water are mixed, ultrasound-assisted enzymolysis is performed, the enzymolysis liquid is separated into solid and liquid, and the clear liquid is concentrated and dried to obtain the caper fruit extract.

6. The composition with moisturizing, soothing and repairing effects according to claim 5, characterized in that: The enzyme is a combination of cellulase, malonase and papain; Preferably, the mass ratio of the cellulase, macerate and papain is (1-5):(1-5):(0.1-2); Preferably, the added amount of the enzyme is 0.1‰-1‰ of the mass of the raw material of Capparis spinosa fruit; Preferably, the mass ratio of the caper fruit raw material to water is 1:(5-40); Preferably, the enzymatic hydrolysis treatment is carried out at 45-55°C for 1-3h; Preferably, the power of the ultrasound is 150-450W.

7. Use of the composition with moisturizing, soothing and repairing effects according to any one of claims 1 to 6 in the preparation of cosmetics; Preferably, the cosmetics include lotions, emulsions, creams, essences, facial masks, sunscreen products, makeup removers, and cleansing products.

8. A cosmetic with moisturizing, soothing and repairing effects, characterized in that: The cosmetic comprises the composition of any one of claims 1 to 6, a polyol, an emollient, an emulsifier, a skin conditioner, a thickener, an antioxidant, and a solvent.

9. The cosmetic with moisturizing, soothing and repairing effects according to claim 8, characterized in that: The skin conditioning agent includes a combination of rice bran oil and shea butter; Preferably, the polyol comprises any one or a combination of at least two of glycerol, butanediol, 1,2-hexanediol or 1,2-pentanediol; Preferably, the emollient comprises any one or a combination of at least two of behenyl alcohol, pentaerythritol tetra(ethylhexanoate), cetearyl alcohol or polydimethylsiloxane; Preferably, the emulsifier comprises any one of cetearyl olivate, sorbitan olivate, cetearyl glucoside or cetearyl alcohol, or a combination of at least two thereof; Preferably, the thickener comprises carbomer and / or ammonium acryloyldimethyltaurate / VP copolymer; Preferably, the antioxidant comprises p-hydroxyacetophenone.

10. The cosmetic with moisturizing, soothing and repairing effects according to claim 8, characterized in that: The cosmetic may further comprise a pH adjuster and / or a preservative.

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