Plant extract composition having skin care effect and use thereof

Abstract

The application discloses a plant extract composition with skin care effect and application thereof, and relates to the technical field of skin care daily chemicals. The plant extract composition with skin care effect provided by the application realizes skin multidimensional care through synergistic compatibility of lipid-soluble components and various aqueous plant extracts. The oily components penetrate the skin to nourish and repair, and the aqueous extracts are used to adjust the skin microenvironment. The two components are compounded to jointly construct an oil control and bacterium inhibition formula; the American ginseng extract enhances the hair follicle activity, and the red clover extract balances the hormone metabolism. The plant extract composition of the application realizes synergistic effects of whitening, antioxidation, barrier repair and microecological regulation while maintaining mildness through the compound compatibility of various substances. Especially, the water-oil phase separation problem of the composition is solved through the nanoemulsion technology, and the bioavailability of active ingredients is improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of skin care daily necessities, in particular to a plant extract composition with skin care effect and application thereof. BACKGROUND

[0002] The scalp, as a special extension of the skin, is the largest organ system of the human body together with the facial and body skin, sharing similar tissue structure and physiological functions, both of which are composed of epidermis, dermis and subcutaneous tissue, and both of which rely on sebaceous glands to secrete sebum to maintain barrier function. However, the density of sebaceous glands on the scalp is significantly higher, about 144-192 sebaceous glands per square centimeter, and this physiological difference makes the scalp more sensitive to environmental stimuli and microbial invasion, and more prone to problems such as imbalance of sebum secretion and dysbiosis. It is worth noting that the health problems on the scalp do not exist in isolation, and once inflammation and other conditions occur, they will often affect the adjacent skin area through complex physiological mechanisms, forming a chain reaction of interrelation between scalp and skin health.

[0003] In the current market products, chemical synthetic anti-dandruff agents (such as ZPT) have the risk of bioaccumulation, and drug preparations such as minoxidil are easy to cause adverse reactions such as contact dermatitis. Although some plant extracts (such as tea tree oil, cypress leaves) have been applied in the field of scalp care, the single active ingredient is often difficult to achieve the synergistic effect of multiple functions such as antioxidant, oil control, repair, etc.

[0004] With the increasing demand of consumers for "natural sources" and "full-effect care", the existing technology faces two major challenges: first, the traditional plant extract compounding system lacks targeted design for scalp microecological regulation; second, most natural ingredients have fragmented mechanisms in the dimensions of oil control, bacteriostasis and barrier repair. Therefore, developing a new plant compounding system to simultaneously regulate skin flora balance, oxidative stress and hair follicle microenvironment through multi-component synergistic effect has become a technical bottleneck that needs to be broken through in the field of skin health. SUMMARY

[0005] The technical problem to be solved by the present application is at least one of the defects mentioned in the background, and the plant extract composition with skin care effect and application thereof are provided, aiming to simultaneously regulate the scalp flora balance, oxidative stress and hair follicle microenvironment through multi-component synergistic effect.

[0006] In order to solve the above problems, the present application proposes the following technical solutions:

[0007] A plant extract composition having skin care effect, comprising at least one of bergamot fruit oil 0.1-1.0 parts, argan kernel oil 0.1-5.0 parts, ginger root oil 0.1-1.0 parts and / or rose oil 0.1-0.5 parts, and at least 5 plant extracts of vanilla extract 0.1-5.0 parts, fig extract 0.1-5.0 parts, chamomile extract 0.1-5.0 parts, fo-ti extract 2.0-5.0 parts, ginger extract 2.0-5.0 parts, cypress leaf extract 2.0-6.0 parts, gardenia flower extract 1.0-5.0 parts, pear flower extract 0.1-1.0 parts, star anise extract 0.1-5.0 parts, tea leaf extract 0.1-1.0 parts, gotu kola leaf extract 3.0-5.0 parts, Japanese late cherry flower extract 3.0-5.0 parts, American ginseng extract 0.1-1.0 parts, red clover extract 1.0-3.0 parts, by weight.

[0008] Further, at least 8 plant extracts of vanilla extract 0.1-5.0 parts, fig extract 0.1-5.0 parts, chamomile extract 0.1-5.0 parts, fo-ti extract 2.0-5.0 parts, ginger extract 2.0-5.0 parts, cypress leaf extract 2.0-6.0 parts, gardenia flower extract 1.0-5.0 parts, pear flower extract 0.1-1.0 parts, star anise extract 0.1-5.0 parts, tea leaf extract 0.1-1.0 parts, gotu kola leaf extract 3.0-5.0 parts, Japanese late cherry flower extract 3.0-5.0 parts, American ginseng extract 0.1-1.0 parts, red clover extract 1.0-3.0 parts are included.

[0009] Further, at least 12 plant extracts of vanilla extract 0.1-5.0 parts, fig extract 0.1-5.0 parts, chamomile extract 0.1-5.0 parts, fo-ti extract 2.0-5.0 parts, ginger extract 2.0-5.0 parts, cypress leaf extract 2.0-6.0 parts, gardenia flower extract 1.0-5.0 parts, pear flower extract 0.1-1.0 parts, star anise extract 0.1-5.0 parts, tea leaf extract 0.1-1.0 parts, gotu kola leaf extract 3.0-5.0 parts, Japanese late cherry flower extract 3.0-5.0 parts, American ginseng extract 0.1-1.0 parts, red clover extract 1.0-3.0 parts are included.

[0010] Further, the bergamot fruit oil, argan kernel oil, ginger root oil and / or rose oil is nanoemulsion, with particle size of 20-200 nm.

[0011] Further, the nanoemulsion has particle size of 20-150 nm, preferably 30-100 nm, more preferably 50-80 nm.

[0012] Further, in the nanoemulsion, the mass ratio of the bergamot fruit oil, the argan kernel oil, the ginger root oil and the damask rose flower oil is 0.1-1:0.5-5:0.1-1:0.1-0.5.

[0013] Further, the butanediol is 3-5 parts, the pentanediol is 0.1-0.8 parts and the deionized water is 20-60 parts.

[0014] The application further provides application of the plant extract composition with the skin care effect in preparation of hair washing and hair care products; the hair washing and hair care products include shampoo, hair conditioner, hair mask and hair care essential oil.

[0015] Further, the addition amount of the plant extract composition with the skin care effect in the hair washing and hair care products is 10-50%wt.

[0016] The application further provides a hair washing product containing the plant extract composition with the skin care effect.

[0017] The application further provides a skin care product containing the plant extract composition with the skin care effect.

[0018] Compared with the prior art, the application can achieve the following technical effects:

[0019] The plant extract composition with the skin care effect provided by the application realizes multi-dimensional care of scalp skin and facial skin through synergistic compatibility of fat-soluble components such as bergamot fruit oil and various aqueous plant extracts. The oily components (such as bergamot fruit oil and argan kernel oil) can penetrate hair follicles to nourish and repair, and the selected aqueous extracts (such as prepared rehmannia root and gotashe grass leaves) can specifically regulate the microenvironment of scalp skin and facial skin. For example, ginger extract is selected to inhibit excessive secretion of sebum, and cypress leaf extract is selected to synergistically inhibit fungal reproduction, thereby building an oil-controlling and bacterium-inhibiting foundation; western ginseng extract enhances hair follicle activity, and red clover extract balances hormone metabolism, thereby forming a hair loss prevention and hair growth support system. The plant extract composition of the application can realize synergistic effects of whitening, antioxidant, barrier repair and microecological regulation while maintaining mildness through the complex compatibility of various substances. In particular, the nanoemulsion technology solves the problem of water-oil phase separation of the composition, and effectively improves the bioavailability of active ingredients. BRIEF DESCRIPTION OF DRAWINGS

[0020] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the following will briefly introduce the drawings needed in the embodiment description. Obviously, the drawings described below are some embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative labor.

[0021] Figure 1 The skin ITA° value test results of the facial essence provided for the use of the application examples 9-11 and the control example 4 are shown in the following table.

[0022] Figure 2 The DPPH scavenging rate test results of the essence and shampoo for the application examples 1-8 and the control examples 1-3 are shown in the following table. DETAILED DESCRIPTION

[0023] The technical solutions in the embodiments of the present application will be described clearly and completely below with reference to the drawings in the embodiments of the present application. Obviously, the embodiments described below are only some of the embodiments of the present application, not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor are within the scope of protection of the present application.

[0024] The plant extract composition provided by the embodiments of the present application has skin care effect and comprises the following ingredients:

[0025] Vanilla extract: obtained from vanilla plants, rich in natural phenolic substances, has the effect of soothing skin sensitivity and regulating microecological balance;

[0026] Fig extract: derived from fig fruits, contains protease and polysaccharide components, can gently clean the skin and enhance the barrier moisturizing capacity;

[0027] Lemon fruit oil extract: extracted from lemon fruits by cold pressing, rich in active ingredients such as limonene, has the effects of oil control, bacteriostasis and sebum secretion regulation;

[0028] Acacia tree kernel oil extract: extracted from acacia tree seeds, contains high concentration of vitamin E and sterols, can deeply nourish hair follicles and repair oxidative damage;

[0029] Golden chamomile extract: obtained from golden chamomile flowers, contains flavonoids, can inhibit the release of inflammatory factors and relieve skin redness, itching and itching;

[0030] Ginger root oil extract: derived from ginger root distillate, improves the nutrition supply of hair follicles by promoting local blood circulation, and assists in preventing hair loss and hair growth;

[0031] Radix Paeoniae Rubra Extract: Extracted from the root of Radix Paeoniae Rubra, it contains flavonoids and volatile oils, which can inhibit the activity of 5α-reductase to reduce excessive oil secretion;

[0032] Ginger Root Extract: Extracted from the root of Zingiber officinale, it is rich in gingerols, which can synergistically inhibit the activity of 5α-reductase to reduce excessive oil secretion;

[0033] Biota Leaf Extract: Extracted from the leaves of Biota orientalis, it contains flavonoids and volatile oils, which have both antifungal activity and promote hair growth;

[0034] Gardenia Flower Extract: Derived from the flower buds of Gardenia jasminoides, it contains iridoid glycosides, which can regulate skin pH and inhibit abnormal keratin proliferation;

[0035] Pear Flower Extract: Extracted from the corolla of Pyrus communis, it contains polyphenols and organic acids, which can gently shrink pores and improve oily skin;

[0036] Freesia Extract: Extracted from the flower part of Freesia hybrida, it contains natural polysaccharides and aromatic substances, which have both moisturizing and soothing effects on skin tightness;

[0037] Tea Leaf Extract: Extracted from the leaves of Camellia sinensis, it contains tea polyphenols and catechins, which can protect the skin from environmental damage by antioxidant and free radical scavenging;

[0038] Centella Asiatica Leaf Extract: Extracted from the whole plant of Centella asiatica, it contains asiaticoside and hydroxy asiatic acid, which can accelerate epidermal repair and strengthen barrier function.

[0039] Japanese Late Cherry Blossom Extract: Derived from the petals of Prunus serrulata, it is rich in sakuranetin and flavonoids, which can improve hair follicle microcirculation and delay scalp aging;

[0040] American Ginseng Extract: Extracted from the roots of Panax quinquefolius, it contains ginsenosides, which can enhance hair papilla cell vitality and prolong hair growth period;

[0041] Red Clover Extract: Extracted from the inflorescences of Trifolium pratense, it contains isoflavones and phenolic acids, which can maintain skin health by regulating hormone metabolism balance;

[0042] Rosa Damascena Flower Oil: Distilled from fresh Rosa damascena flowers, it contains active ingredients such as rose ether, which can improve dry skin and enhance moisturizing and water retention capacity.

[0043] Table 1 provides the plant extract composition formula for each experimental group in the present application, with a total amount of 100wt%;

[0044] Table 1 provides the plant extract composition formula for each experimental group in the present application, with a total amount of 100wt%;

[0045]

[0046] The skin care plant extract compositions of experimental groups 1-5 and comparative group 1 were prepared by conventional mechanical mixing; the skin care plant extract compositions of experimental groups 6-11 were prepared by mixing the nanoemulsion with butylene glycol / pentylene glycol in advance at a ratio, and slowly adding other plant extracts under low-temperature and low-speed (40°C, 500 rpm / 10 min) stirring.

[0047] The oily ingredients (bergamot fruit oil, argan kernel oil, ginger root oil, and damask rose flower oil) were mixed at a predetermined ratio to prepare a nanoemulsion based on the formula of experimental groups 6-11:

[0048] The nanoemulsion of the oily ingredient of experimental group 6 had a particle size of 50 nm±5 nm;

[0049] The formula of experimental group 7 was the same as that of experimental group 3, except that the nanoemulsion of the oily ingredient had a particle size of 20 nm±5 nm;

[0050] The formula of experimental group 8 was the same as that of experimental group 3, except that the nanoemulsion of the oily ingredient had a particle size of 80 nm±5 nm;

[0051] The formula of experimental group 9 was the same as that of experimental group 3, except that the nanoemulsion of the oily ingredient had a particle size of 150 nm±5 nm;

[0052] The formula of experimental group 10 was the same as that of experimental group 4, except that the nanoemulsion of the oily ingredient had a particle size of 80 nm±5 nm;

[0053] The formula of experimental group 11 was the same as that of experimental group 4, except that the nanoemulsion of the oily ingredient had a particle size of 50 nm±5 nm.

[0054] The preparation process of the nanoemulsion is as follows:

[0055] The argan kernel oil was dehydrated at 60°C under vacuum (-0.08 MPa) for 30 min, and filtered to remove suspended impurities (pore size 0.22 μm);

[0056] The bergamot fruit oil, argan kernel oil, ginger root oil, and / or damask rose flower oil were mixed at a ratio in the formula at 65°C, and 0.05% tocopherol was added to prevent oxidation, to obtain an oil phase;

[0057] Deionized water was mixed with butylene glycol and pentylene glycol, the pH was adjusted to 5.4-5.6, and preheated to 65°C; 3-8% emulsifier (sucrose ester: lecithin mass ratio = 2:1) was added to ensure that the HLB value (12-14) matched the polarity of the oil phase, to obtain an aqueous phase;

[0058] The oil phase is slowly added into the water phase, and emulsified at 8000 rpm for 5 min under high shear to obtain a pre-emulsified liquid; the homogenization pressure and times are adjusted to perform multi-stage homogenization to obtain nanolipid with target particle size.

[0059] For example, the first-stage homogenization is 80 MPa x 3 times, and the second-stage homogenization is 120 MPa x 5 times. The homogenization parameters are well known to those skilled in the art, and are not limited in the present application.

[0060] It should be noted that the components mentioned in the above preparation process are not present in the formula, and accordingly, no addition is required in the process.

[0061] The stability of the skin care plant extract compositions prepared in the above experimental groups 1-11 is tested, and the test method is as follows:

[0062] The samples are placed in a 40℃ constant temperature box for 90 days, and the particle size of the nanolipid in the system is detected by a laser particle size analyzer at 0th day, 30th day, 60th day and 90th day, respectively. The results are shown in Table 2:

[0063] Table 2 Stability results

[0064]

[0065] As can be seen from the results in Table 2, the skin care plant extract composition of the present application contains oil phase material and water phase material. The simple way of conventional mechanical mixing occurs delamination within 30 days of storage in a 40℃ constant temperature box, and the system is extremely unstable. However, after the oil phase material is wrapped in the form of nanolipid and mixed with the water phase material, no delamination phenomenon is observed within 90 days of the test period, indicating that wrapping the oil phase material with nanolipid helps to improve the stability of the system.

[0066] Further, it is found that the particle size of the nanolipid in experimental group 7 (20 nm) increases by 100% in 90 days, and the particle size of the nanolipid in experimental group 9 (150 nm) increases by 20%. However, the particle size of the nanolipid in the 50-80 nm group (experimental groups 6 / 8 / 10) increases by ≤5%, proving that the stability of the nanolipid with a particle size of 50-80 nm is optimal. The change in the particle size of the nanolipid in experimental group 7 and experimental group 9 is more than 15%. Obviously, the particle size of the nanolipid is too small (20 nm ± 5 nm) or too large (150 nm ± 5 nm), which will have a negative impact on the stability of the system. In combination with the process parameters, the particle size of the nanolipid is best at 50-80 nm.

[0067] The retention of the main active ingredients in the plant extract compositions of experimental groups 6, 8, 10 and comparative group 1 stored in a 40℃ constant temperature box for 90 days is detected. The formula of the comparative group 1 is the same as that of experimental group 6, except that it does not contain the oil phase component. The results are shown in Table 3:

[0068] Table 3 Active ingredient retention rate of experimental groups 6, 8, 10 and comparative group 1

[0069]

[0070] Active ingredient retention rate = active ingredient content on day 90 / active ingredient content on day 0 * 100%

[0071] It can be seen that the skin care plant extract composition of the present application nano-wraps the oil phase material, and then compounding the water phase material, the system can be stable for a long time, and the retention rate of the active ingredient is above 85%.

[0072] Application Example 1

[0073] A scalp care serum has the following formula in Table 4, and the preparation process is as follows: dissolve ethanol, phenoxyethanol and daily fragrance completely, then add the skin care plant extract composition and water to dissolve completely;

[0074] Table 4 Formula of serum examples 1-4 and control example 1 (wt%)

[0075]

[0076] The skin care plant extract composition used in serum examples 1-4 is the skin care plant extract composition of experimental group 8.

[0077] Application Example 2

[0078] A scalp care shampoo has the following formula in Table 5, and the preparation process is as follows:

[0079] S1. Add EDTA disodium, hydroxypropyl guar hydroxypropyltrimonium chloride, polyquaternium-10, citric acid into water, stir to disperse uniformly, and heat to 85°C;

[0080] S2. Add sodium laureth sulfate, cocamidopropyl betaine, cocoglycoside into the reaction kettle, 0.1-1% PEG-150 pentaerythrityl tetraisostearate, PPG-2 hydroxyethyl cocamide, cocamide methyl MEA, and stir for 20 minutes;

[0081] S2. After cooling to 45°C, add the plant extract composition, glycerol, phenoxyethanol, sodium benzoate, PPG-3 cetyl ether, and make up the water content;

[0082] S3. Stand still to defoam, and the scalp care shampoo is obtained after inspection;

[0083] Table 5 Formula of shampoo examples 5-8 and control examples 2-3 (wt%)

[0084]

[0085] The skin care plant extract composition used in shampoo examples 5-8 is the skin care plant extract composition of experimental group 6.

[0086] Application example 3

[0087] A facial serum is prepared according to the following table 6, the preparation process is: dissolving glycerin, butylene glycol, betaine, nicotinamide, allantoin and hydroxybenzoic acid ester completely, then adding the skin care plant extract composition and dissolving completely in water;

[0088] Table 6 Formulation of facial serum examples 9-11 and control example 4 (wt%)

[0089]

[0090] The skin care plant extract composition used in facial serum examples 9-11 is composed of 0.8% bergamot fruit oil, 0.5% musk rose flower oil, 2.5% vanilla extract, 3.0% golden yarrow extract, 2.0% gardenia flower extract, 0.6% pear flower extract, 1.5% star aniseed extract, 3.8% gotu kola leaf extract, 2.5% red clover extract, 4.0% butylene glycol, 0.5% pentylene glycol, and the balance is deionized water, by mass percentage. Among them, the bergamot fruit oil and the musk rose flower oil are nanoemulsions with a particle size of 50 nm ± 5 nm.

[0091] Head skin and facial skin care efficacy test

[0092] Head skin care efficacy test: 220 healthy male or female subjects with fine and soft hair, more hair loss and mild hair thinning are recruited to use the test samples (examples 1-8 and control examples 1-3), 20 people for each sample, and the head skin water loss amount (TEWL) is determined, the head skin oil content, the head skin moisture content, the consumer self-evaluation, and the comprehensive evaluation are carried out by instrument detection and self-evaluation questionnaire methods after continuous use of the test samples for 4 weeks, and the average value is taken;

[0093] Facial skin care efficacy test: 120 healthy male or female subjects aged 25-35 are recruited to use the test samples (examples 9-11 and control example 4), 30 people for each sample, and the facial skin moisture content, the water loss amount (TEWL), and the skin ITA° value are determined by instrument detection method after continuous use of the test samples for 4 weeks, and the average value is taken;

[0094] The results of the skin moisture content determination are shown in Table 7, which shows that the facial serum water added with the skin care plant extract composition of the present application has excellent moisturizing effect after four weeks of use, and the moisturizing effect is positively correlated with the added amount;

[0095] Table 7 Moisture content results of facial serum water examples 9-11 and control examples 4

[0096]

[0097] Measurement of the amount of water loss (TEWL) of scalp skin and facial skin

[0098] The amount of water loss (TEWL) of scalp skin and facial skin is an important parameter for evaluating the water protection layer function of scalp skin and facial skin, and can be used to evaluate the strength of the barrier function of scalp skin and facial skin. If the barrier function of scalp skin or facial skin is damaged, TEWL will increase, and symptoms such as dryness, desquamation, sensitivity, etc. will occur in both scalp skin and facial skin. If the protection layer of scalp skin and facial skin is better, the moisture content is higher, and the value of water loss TEWL is lower. The unit of TEWL is: g / hm 2 ;

[0099] Table 8 is the TEWL test results of application examples 1-11 and control examples 1-4

[0100]

[0101] As can be seen from Table 8, the scalp care serum prepared by the present application has a good repairing effect on the barrier function of scalp skin. After 4 weeks of use by consumers, the scalp serum and shampoo of the present application have a small amount of water loss of the skin, indicating that the synergistic effect of the compounding of the components can enhance the barrier function of the scalp. In addition, the TEWL improvement rate of application example 4 (25% addition amount) (-25.92%) is significantly higher than that of application example 3 (10% addition amount, -15.66%), indicating that the barrier repair effect is better when the addition amount of the plant extract composition in the product is ≥25%.

[0102] At the same time, the facial serum water provided by the present application also has a good barrier function repairing effect on facial skin, and the higher the addition amount of the plant extract composition in the product, the less the transdermal water loss, indicating that the barrier function of the skin is better.

[0103] Figure 1The results of the skin ITA° value test of the facial essence provided by the examples 9-11 of the present application and the control example 4 show that the skin ITA° value of the consumer is greater when the essence added with the skin care composition is used than when the control example 4 product without the added composition is used, indicating that the facial care product added with the plant extract composition of the present application has excellent whitening effect.

[0104] Sebum content test of the scalp

[0105] Table 9 is the sebum content test results of the essence and shampoo of the examples 1-8 and the control examples 1-3

[0106]

[0107] After continuous use of the scalp care essence and the scalp care shampoo test samples for 4 weeks, the sebum content of the scalp of the subjects is significantly reduced compared with the base value, and the scalp care composition has obvious oil control effect compared with the control group.

[0108] Consumer self-evaluation

[0109] The self-evaluation is mainly based on the following dimensions by using the scoring system;

[0110] 1. After 4 weeks of sample use, the scalp is more comfortable (refreshing / not tight / reduced oil secretion);

[0111] 2. After 4 weeks of sample use, hair loss is reduced when washing and combing the hair;

[0112] 3. After 4 weeks of sample use, the scalp feels relaxed and not tight;

[0113] 4. After 4 weeks of sample use, the dandruff condition is improved;

[0114] 5. After 4 weeks of sample use, the scalp dry itching is relieved;

[0115] 6. The feeling of increased hair volume and improved hair volume is improved;

[0116] 7. The sample is mild and non-irritating;

[0117] 8. The overall satisfaction with the sample.

[0118] Among them, 1 point means very dissatisfied; 2 points means not satisfied; 3 points means general; 4 points means satisfied; 5 points means very satisfied.

[0119] Scores of 4-5 points are considered satisfactory, and the results in Table 10 are the proportion of satisfied people;

[0120] Table 10 is the consumer evaluation results of the essence and shampoo of the examples 1-8 and the control examples 1-3

[0121]

[0122] It can be seen that the overall satisfaction of consumers is more, up to 100%, by adding the scalp care serum and the scalp care shampoo of the plant extract composition of the present application. Compared with the control example, it has better effects on dandruff improvement, scalp soothing, scalp itching relief, and hair loss prevention, etc.

[0123] In vitro bacteriostatic efficacy determination

[0124] In order to investigate the anti-dandruff effect of the scalp care serum and the shampoo of the present application, the in vitro bacteriostatic efficacy determination was carried out on the serum of the control example 1 and the shampoo of the control example 2 without adding the composition, the control example 3 without adding the composition but adding the anti-dandruff agent piroctone olamine salt. The standard strain of Malassezia furfur was selected, and the bacteriostatic effect was detected by the bacteriostatic ring method. If the diameter of the bacteriostatic ring is greater than 7 mm, the product has bacteriostatic effect. If the diameter of the bacteriostatic ring is not greater than 7 mm, the product has no bacteriostatic effect. The bacteriostatic results are as follows:

[0125] Table 11 is the bacteriostatic results of the serum and the shampoo of the application examples 1-8 and the control examples 1-3

[0126]

[0127] The results show that the plant extract composition of the present application applied in the scalp care serum and the scalp care shampoo has excellent inhibition effect on Malassezia furfur, and its bacteriostatic effect exceeds that of the same shampoo system adding 0.3% of the anti-dandruff agent piroctone olamine salt.

[0128] Mildness test:

[0129] This test takes advantage of the characteristics of the complete, clear and transparent blood vessel system of the mid-stage chorioallantoic membrane of the hatched chicken embryo. A certain amount of the test substance is directly contacted with the chorioallantoic membrane of the chicken embryo, and after a period of action, the changes of the toxicity effect indicators (such as bleeding, coagulation and vascular lysis) of the chorioallantoic membrane are observed. These indicators reflect the changes of the morphological structure, color and permeability of the blood vessels and vascular network, as well as the phenomena and the degree of damage of the protein denaturation of the chorioallantoic membrane. Finally, a score is obtained for the evaluation of the eye irritation of the test substance. The experimental results are shown in Table 12.

[0130] Experimental steps:

[0131] S1. Preparation of the test substance

[0132] S1.1. Liquid test substance

[0133] The test shall be performed as a reaction time test with the undiluted original test item; non-transparent test items (turbid and coloured suspensions) shall be tested as a endpoint evaluation test; non-transparent turbid test items can be dissolved / diluted with an appropriate solvent to a clear solution at the highest concentration and tested as a reaction time test. The test was performed as a endpoint evaluation test;

[0134] S1.2. Controls and preparation

[0135] S1.2.1 Negative control: A 0.9% sodium chloride solution is usually chosen as a negative control for rinsing and as a negative control during the test;

[0136] S1.2.2 Positive control: A known eye irritant shall be used as a positive control for each test. If the test is intended to identify corrosive or severe irritant substances, the positive control shall be a substance which produces a severe reaction, e.g. 1% SDS, 0.1 mol / L sodium hydroxide. If the test is intended to assess the severity of eye irritation of the test item, a positive control which produces a less severe reaction can be used, e.g. acetic acid at different concentrations;

[0137] S1.2.3 Solvent control: If the test item is diluted with a solvent, a solvent control shall be included in the test;

[0138] S1.2.4 Reference control: Mainly used to demonstrate the effectiveness of the test method, in particular to check the reactivity of each batch of chicken embryos, to test turbid / strange / exceptional eye irritant reactions of test items, or to assess the relative eye irritant potential of an eye irritant. Reference control substances shall have the following properties: stable and reliable source, chemical class similar to the test item, known physical / chemical properties, known effect in the rabbit eye test in vivo, known extent of the expected reaction.

[0139] S2. Preparation of CAM

[0140] The 9-day old embryos are examined for infertility, lack of activity, defects, and thin shell. The air sacs are marked with a pen and opened with a mini-cutter. The remaining shell is removed from the air sacs along the marked opening. Care is taken not to damage the integrity of the chorioallantoic membrane. The membrane is moistened with a pipette and the inner membrane is removed with forceps, taking care not to damage the blood vessels. The blood vessel system is again observed for integrity and, if intact, the next step of the test is performed.

[0141] S3. Observation

[0142] For testing of solid and turbid liquid test substances in the form of fine particles, granules, pastes, etc. Take 0.3 mL of the extruded solid, fine particles or granules (already ground to fine particles) and apply directly to the CAM, ensuring that at least 50% of the CAM surface is covered with the test substance. After 3 minutes, rinse the test substance from the CAM membrane with physiological saline and observe the results within 30 seconds after rinsing is complete. Observe the extent of each of the toxic effects. If the observations show at least one reaction with a score of moderate or greater in all 6 embryos, the test should be repeated.

[0143] S4. Observation of the results

[0144] S4.1 Bleeding

[0145] Bleeding refers to the escape of blood from the blood vessels and / or capillaries of the CAM. Bleeding can take various forms, such as cauliflower-like, smooth, diffuse gauze-like or punctiform bleeding (due to selective escape of blood from different areas of the vascular membrane); it is graded and scored according to the severity.

[0146] a) No bleeding (0 points);

[0147] b) Mild bleeding (1 point): only fine vessels are seen to bleed and there is a small amount of bleeding (e.g. 0.5% Texapon ASV for 5 minutes);

[0148] c) Moderate bleeding (2 points): small and large vessels are seen to bleed and there is a significant amount of bleeding (e.g. 1.0% Texapon ASV for 5 minutes);

[0149] d) Severe bleeding (3 points): almost all the vessels are seen to bleed and there is a large amount of bleeding (e.g. 5% Texapon ASV for 5 minutes). It should be noted that the bleeding can be transient and the large amount of bleeding observed in the first 30 seconds can cover the subsequent bleeding reaction.

[0150] S4.2 Coagulation

[0151] Coagulation refers to the denaturation of proteins inside and outside the blood vessels, usually only seen in large and medium-sized vessels, excluding changes in capillaries. Thrombosis: this is the intravascular coagulation, which is the interruption of blood flow inside the blood vessels due to different causes, such as changes in blood vessel pressure, swelling of the vessel wall, etc., and is manifested as dark coagulation points inside the blood vessels. Extravascular coagulation: this can be manifested as dark coagulation points outside the blood vessels; it can also be manifested as turbidity (opacity) that appears on all or part of the membrane, which can be similar to a milky gauze or opalescent. Care should be taken not to confuse coagulation with changes in the physicochemical properties of the test substance in aqueous solution (e.g. formation of gels, precipitates, etc.). Coagulation is graded and scored according to the severity.

[0152] a) No coagulation (0 points);

[0153] b) mild coagulation (1 point): mild coagulation in blood vessels and / or in blood vessels outside, and / or mild turbidity of CAM membrane (mild coagulation such as 0.2% sodium hydroxide for 5 min, mild turbidity such as 0.3% acetic acid for 5 min);

[0154] c) moderate coagulation (2 points): moderate coagulation in blood vessels and / or in blood vessels outside, and / or moderate turbidity of CAM membrane (moderate coagulation such as 0.3% sodium hydroxide for 5 min, moderate turbidity such as 3% acetic acid for 5 min);

[0155] d) severe coagulation (3 points): severe coagulation in blood vessels and / or in blood vessels outside, and / or severe turbidity of CAM membrane (severe coagulation such as 0.5% sodium hydroxide for 5 min, severe turbidity such as 30% acetic acid for 5 min).

[0156] S4.3 Vessel resolution

[0157] Vessel resolution refers to the disappearance of blood vessels on the CAM membrane, which can be caused by various factors such as bleeding, changes in blood vessel wall tension, etc. Vessel resolution is classified and scored according to the severity.

[0158] a) no vessel resolution (0 point);

[0159] b) mild vessel resolution (1 point): only small blood vessels are resolved (such as 0.5% Texapon ASV for 5 min);

[0160] c) moderate vessel resolution (2 points): small blood vessels and large blood vessels are resolved (such as 1% Texapon ASV for 5 min);

[0161] d) severe vessel resolution (3 points): large blood vessels and all blood vessel trees are resolved (such as 5% Texapon ASV for 5 min).

[0162] S5. Endpoint score method (ES)

[0163] The test using the endpoint evaluation method is calculated as the endpoint score (ES), and the results are kept to two decimal places:

[0164] ES = the sum of the degree of bleeding, coagulation, and vessel resolution observed in 6 chicken embryos, and the eye irritation of the test substance is classified according to the ES value.

[0165] Endpoint score irritation classification:

[0166] ES≤12 indicates no / mild irritation;

[0167] 12 < ES < 16 indicates moderate irritation;

[0168] ES≥16 indicates strong irritant / corrosive properties.

[0169] Table 12 shows the mildness test results of the serums and shampoos used in Examples 1-8 and Comparative Examples 1-3.

[0170]

[0171] It can be seen that the mildness test results of the essence and shampoo prepared by this invention are ES≤12, which means they are non-irritating / mildly irritating and have excellent mildness.

[0172] DPPH free radical scavenging test:

[0173] In this experiment, the DPPH free radical scavenging tests were conducted on the serums and shampoos used in Examples 1-8 and Control Examples 1-3.

[0174] The testing principle is as follows: DPPH is a stable organic free radical with its strongest absorption peak at 519 nm. When this free radical is added to an ethanol solution, it turns a deep purple color. When a certain amount of antioxidant is added, the absorbance value at 519 nm can be measured after the antioxidant removes DPPH, causing the color to lighten. Quantitative analysis using spectrophotometry is employed to detect the free radical scavenging effect, thereby evaluating the ability of the active ingredient with antioxidant properties in the product to scavenge free radicals. A higher scavenging rate indicates a stronger antioxidant capacity. The blank sample had a DPPH scavenging rate of 0. Finally, the test results of the DPPH free radical scavenging ability of the serums and shampoos in Examples 1-8 and Control Examples 1-3 were obtained, as follows: Figure 2 As shown.

[0175] Depend on Figure 2 The data show that the scalp care serums and shampoos used in Examples 1-8 all have a strong ability to scavenge free radicals. Among them, the serum in Example 4, which added 25% of the scalp care composition, has a better advantage in scavenging free radicals, while the shampoos in Examples 7 and 8, which added 20% and 17% of the scalp care composition respectively, have better free radical scavenging effects.

[0176] Understandably, the synergistic effect of small-particle nanoemulsions with other components helps plant extracts penetrate the skin surface to exert their effects.

[0177] In the above embodiments, the descriptions of each embodiment have different focuses. For parts that are not described in detail in a certain embodiment, please refer to the relevant descriptions in other embodiments.

[0178] The above is a specific embodiment of the present application, but the protection scope of the present application is not limited thereto, and any person skilled in the art can easily think of various equivalent modifications or replacements within the technical scope disclosed by the present application, and these modifications or replacements should be covered within the protection scope of the present application. Therefore, the protection scope of the present application should be subject to the protection scope of the claims.

Claims

1. A plant extract composition with skin-care properties, characterized in that, Based on weight, it includes at least one of the following oily components: 0.1-1.0 parts of bergamot fruit oil, 0.1-5.0 parts of Argan kernel oil, 0.1-1.0 parts of ginger root oil and / or 0.1-0.5 parts of Rosa damascena flower oil; At least five of the following water-based plant extracts: vanilla extract 0.1-5.0 parts, fig extract 0.1-5.0 parts, golden chamomile extract 0.1-5.0 parts, processed Polygonum multiflorum extract 2.0-5.0 parts, ginger root extract 2.0-5.0 parts, Platycladus orientalis leaf extract 2.0-6.0 parts, gardenia flower extract 1.0-5.0 parts, pear blossom extract 0.1-1.0 parts, freesia extract 0.1-5.0 parts, tea extract 0.1-1.0 parts, centella asiatica leaf extract 3.0-5.0 parts, Japanese cherry blossom extract 3.0-5.0 parts, American ginseng extract 0.1-1.0 parts, and red clover extract 1.0-3.0 parts; The oily component is in the form of a nanoemulsion with a particle size of 50-80 nm. The preparation process of nanoemulsions is as follows: Argan kernel oil was dehydrated at 60℃ under vacuum and -0.08MPa for 30 min, and suspended impurities were removed by filtration with a pore size of 0.22μm. According to the proportions in the formula, bergamot fruit oil, argan kernel oil, ginger root oil and / or Rosa damascena flower oil are mixed at 65°C, and 0.05% tocopherol is added to prevent oxidation to obtain the oil phase; Deionized water is mixed with butanediol and pentanediol, and the pH is adjusted to 5.4-5.

6. The mixture is then preheated to 65°C. 3-8% emulsifier is added, with a sucrose ester:lecithin mass ratio of 2:1, ensuring that the HLB value is within the range of 12-14 to match the polarity of the oil phase, thus obtaining the aqueous phase. The oil phase was slowly added to the aqueous phase and emulsified under high-speed shear at 8000 rpm for 5 min to obtain a pre-emulsified liquid; the homogenization pressure and number of homogenization were adjusted to perform multi-stage homogenization to obtain nanoemulsions with the target particle size. If any of the components mentioned in the above preparation process are not present in the formulation, they do not need to be added in the process.

2. The plant extract composition with skin-care effects as described in claim 1, characterized in that, It includes at least eight plant extracts: vanilla extract (0.1-5.0 parts), fig extract (0.1-5.0 parts), golden chamomile extract (0.1-5.0 parts), processed Polygonum multiflorum extract (2.0-5.0 parts), ginger root extract (2.0-5.0 parts), Platycladus orientalis leaf extract (2.0-6.0 parts), gardenia flower extract (1.0-5.0 parts), pear blossom extract (0.1-1.0 parts), freesia extract (0.1-5.0 parts), tea extract (0.1-1.0 parts), centella asiatica leaf extract (3.0-5.0 parts), Japanese cherry blossom extract (3.0-5.0 parts), American ginseng extract (0.1-1.0 parts), and red clover extract (1.0-3.0 parts).

3. The plant extract composition with skin-care effects as described in claim 1, characterized in that, It includes at least 12 plant extracts: vanilla extract 0.1-5.0 parts, fig extract 0.1-5.0 parts, golden chamomile extract 0.1-5.0 parts, processed Polygonum multiflorum extract 2.0-5.0 parts, ginger root extract 2.0-5.0 parts, Platycladus orientalis leaf extract 2.0-6.0 parts, gardenia flower extract 1.0-5.0 parts, pear blossom extract 0.1-1.0 parts, freesia extract 0.1-5.0 parts, tea extract 0.1-1.0 parts, centella asiatica leaf extract 3.0-5.0 parts, Japanese cherry blossom extract 3.0-5.0 parts, American ginseng extract 0.1-1.0 parts, and red clover extract 1.0-3.0 parts.

4. The plant extract composition with skin-care effects as described in claim 1, characterized in that, In the nanoemulsion, the mass ratio of bergamot fruit oil, argan kernel oil, ginger root oil, and Rosa damascena flower oil is 0.1-1:0.5-5:0.1-1:0.1-0.

5.

5. The plant extract composition with skin-care effects as described in claim 1, characterized in that, It also includes 3-5 parts of butanediol, 0.1-0.8 parts of pentanediol, and 20-60 parts of deionized water.

6. The application of the plant extract composition with skin care function as described in any one of claims 1-5 in the preparation of shampoo, hair care and skin care products; wherein the shampoo and hair care products include shampoo, conditioner, hair mask and hair oil, and the skin care products include water, lotion, cream and serum.

7. The application as described in claim 6, characterized in that, The plant extract composition with skin-care properties is added to shampoo and hair care products at a rate of 10-50%wt.

8. A shampoo product, characterized in that, The composition includes plant extracts with skin-care effects as described in any one of claims 1-5.

9. A skincare product, characterized in that, The composition includes plant extracts with skin-care effects as described in any one of claims 1-5.

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