Electromagnetic wave sludge reduction community structure regulation and control method for improving microbial adhesion

Abstract

The invention relates to the technical field of sewage treatment, in particular to an electromagnetic wave sludge reduction community structure regulation and control method for improving microorganism adhesion. The method comprises the following steps: 1) treating sewage by adopting an A / A / O reactor, and stably operating for a period of time; 2) introducing part of the sludge obtained in the secondary sedimentation tank as return sludge into the anaerobic tank, and continuously exciting the return sludge by electromagnetic waves to improve the surface performance of the sludge in the anaerobic tank, the anoxic tank and the aerobic tank; 3, part of the nitrification liquid in the aerobic tank serves as backflow nitrification liquid to be introduced into the anoxic tank, and the backflow nitrification liquid is continuously excited by electromagnetic waves so as to improve the surface performance of sludge in the anaerobic tank, the anoxic tank and the aerobic tank. By loading the electromagnetic waves, the adhesion characteristic of microorganisms can be improved, and the sludge reduction effect can be improved.

Description

Technical Field

[0001] The present invention relates to the technical field of sewage treatment, and in particular to an electromagnetic wave sludge reduction community structure regulation method for improving microbial adhesion. Background Art

[0002] During the biological treatment of wastewater, the formation and stability of functional microbial aggregates are key factors in determining the system's treatment performance. High levels of microbial aggregation not only enhance metabolic synergy among microbial communities, improving the system's robustness and stability, but also effectively promote the growth and reproduction of functional microorganisms, thereby enhancing the overall efficiency of the treatment system. Previous studies have shown that the formation of functional aggregates is primarily influenced by the adhesion between microorganisms and between microorganisms and non-biological components. Therefore, microbial adhesion has become a core parameter for regulating the treatment efficiency of sludge systems. Its enhancement can effectively promote the enrichment and stabilization of dominant bacterial communities, providing important support for achieving sludge reduction.

[0003] Among the numerous technologies that influence microbial adhesion, electromagnetic wave loading has garnered significant attention in recent years due to its significant advantages, including non-contact nature, highly controllable parameters, rapid response, and low energy consumption. This technology can effectively manipulate microbial adhesion behavior and surface properties, thereby influencing the structure and functional expression of aggregates, without significantly disrupting existing bioprocesses. This technology demonstrates excellent process compatibility and promising application prospects.

[0004] Therefore, developing sludge adhesion performance control technology based on electromagnetic wave loading and constructing an efficient, stable and low-carbon sludge reduction treatment process has become one of the important research directions that urgently need breakthroughs in the field of sewage treatment. Summary of the Invention

[0005] The technical problem to be solved by the present invention is to address the deficiencies of the above-mentioned prior art and provide a method for regulating the community structure of sludge reduction by electromagnetic waves to enhance the adhesion of microorganisms.

[0006] The technical solution of the present invention to solve the above technical problems is as follows:

[0007] An electromagnetic wave sludge reduction community structure control system for improving microbial adhesion comprises: an A / A / O reactor comprising an anaerobic tank (1), an anoxic tank (2) and an aerobic tank (3) connected in sequence;

[0008] A water inlet pipe connected to the anaerobic tank (1);

[0009] A secondary sedimentation tank (4) connected to the aerobic tank (3), wherein the secondary sedimentation tank (4) has a mud outlet;

[0010] A first electromagnetic wave loading unit (5) having a first flow channel, wherein both ends of the first flow channel are connected to the water inlet pipeline and the mud outlet via a mud return pipe;

[0011] A second electromagnetic wave loading unit (6) having a second flow channel, wherein both ends of the second flow channel are connected to the anoxic tank (2) and the aerobic tank (3) via nitrification liquid return pipes;

[0012] and an original return sludge pipe (7) communicating with the sludge outlet and the water inlet pipeline.

[0013] Based on the above technical solution:

[0014] The first electromagnetic wave loading unit can continuously excite the returned sludge with electromagnetic waves, which can improve the surface properties of the sludge in the anaerobic tank, anoxic tank and aerobic tank;

[0015] The second electromagnetic wave loading unit can continuously excite the refluxed nitrification liquid with electromagnetic waves, which can further improve the surface properties of the sludge in the anaerobic tank, anoxic tank and aerobic tank;

[0016] The stimulated return sludge and stimulated return nitrification liquid work synergistically in the A / A / O reactor, which can significantly improve the surface properties of sludge in anaerobic tanks, anoxic tanks and aerobic tanks, enhance the adhesion of microorganisms and promote sludge reduction.

[0017] Furthermore, a microporous aeration disk is provided in the aerobic tank (3), and the microporous aeration disk is connected to an air pump.

[0018] Furthermore, a peristaltic pump is provided in communication between the first electromagnetic wave loading unit (5) and the water inlet pipeline.

[0019] Furthermore, a peristaltic pump is provided in communication between the second electromagnetic wave loading unit (6) and the anoxic tank (2).

[0020] The present invention also provides an electromagnetic wave sludge reduction community structure control method for improving microbial adhesion, which uses the electromagnetic wave sludge reduction community structure control system for improving microbial adhesion, comprising the following steps:

[0021] 1) Use A / A / O reactor to treat sewage and operate stably for a period of time;

[0022] 2) A portion of the sludge obtained from the secondary sedimentation tank is introduced into the anaerobic tank as return sludge. The return sludge is continuously excited by electromagnetic waves to improve the surface properties of the sludge in the anaerobic tank, anoxic tank, and aerobic tank and reduce the abundance of flagellar synthesis genes;

[0023] 3) Part of the nitrification liquid from the aerobic tank is passed into the anoxic tank as return nitrification liquid. The return nitrification liquid is continuously excited by electromagnetic waves to improve the surface properties of the sludge in the anaerobic tank, anoxic tank, and aerobic tank, and to reduce the abundance of flagellar synthesis genes and increase the abundance of fimbriae synthesis genes.

[0024] Based on the above technical solution:

[0025] The first electromagnetic wave loading unit can continuously excite the returned sludge with electromagnetic waves, which can improve the surface properties of the sludge in the anaerobic tank, anoxic tank and aerobic tank;

[0026] The second electromagnetic wave loading unit can continuously excite the refluxed nitrification liquid with electromagnetic waves, which can further improve the surface properties of the sludge in the anaerobic tank, anoxic tank and aerobic tank;

[0027] The stimulated return sludge and stimulated return nitrification liquid work synergistically in the A / A / O reactor, which can significantly improve the surface properties of sludge in anaerobic tanks, anoxic tanks and aerobic tanks, enhance the adhesion of microorganisms and promote sludge reduction.

[0028] Specifically:

[0029] The power of continuous electromagnetic wave excitation for return sludge is 265-285 W;

[0030] The continuous excitation time of electromagnetic waves on the return sludge is 45-55 s;

[0031] Return sludge accounts for 20-30% of the total sludge;

[0032] The frequency of continuous excitation of electromagnetic waves for return sludge is 2400-2500 MHz.

[0033] Specifically:

[0034] The power of continuous electromagnetic excitation of the reflux nitrification solution is 410-430 W;

[0035] The time of continuous excitation of electromagnetic waves on the reflux nitrification solution is 65-75 s;

[0036] The reflux nitrification liquid accounts for 10-20% of the total nitrification liquid;

[0037] The frequency of the continuous excitation of electromagnetic waves on the reflux nitrification liquid is 2400-2500 MHz.

[0038] Specifically:

[0039] After the A / A / O reactor has been running stably for 25-35 days, continuous electromagnetic wave excitation is performed;

[0040] Working temperature is 20-30 ℃;

[0041] The dissolved oxygen in the aerobic tank is 1.90-2.10 mg / L;

[0042] The sludge return ratio is 65-75%;

[0043] The reflux ratio of nitrification liquid is 240-260%;

[0044] The sludge retention time is 14-16 days.

[0045] Specifically: the surface properties of the sludge include any one or more of the following:

[0046] The contact angle of anaerobic tanks is improved by 24-25%, that of anoxic tanks by 42-43%, and that of aerobic tanks by 32-33%;

[0047] Reduce the α-helix / (β-sheet + random coil) value;

[0048] Surface roughness: anaerobic tanks can be improved by up to 195-200%, anoxic tanks can be improved by up to 105-110%, and aerobic tanks can be improved by up to 275-285%.

[0049] Specifically, in step 2), the abundance of flagellar synthesis genes in the anaerobic, anoxic, and aerobic tanks decreased by 9-10%.

[0050] Specifically, in step 3), the abundance of flagellar synthesis genes in the anaerobic, anoxic, and aerobic tanks decreased by 27-28%, and the abundance of fimbriae synthesis genes increased by 13-14%.

[0051] The beneficial effects of the present invention are:

[0052] 1. The present invention uses electromagnetic wave collaborative loading technology to treat external return sludge and internal return nitrification liquid. By increasing the sludge contact angle, changing the protein secondary structure and improving the sludge surface roughness, it effectively improves the interaction force and hydrophobicity of the sludge surface, creating a more favorable interface environment for microbial adhesion and aggregation.

[0053] 2. The present invention uses electromagnetic wave synergistic loading to directionally regulate the expression of attachment genes in functional bacterial communities, thereby downregulating the expression of genes related to flagellar movement and movement and upregulating the expression of fimbriae genes, thereby enhancing the attachment ability of microorganisms.

[0054] 3. This invention uses purely physical control methods, effectively avoiding the problem of drug residue caused by traditional chemical conditioning methods, and there is no risk of secondary contamination. The electromagnetic wave parameters can be precisely controlled, the operation is simple, and it is easy to achieve automated control. BRIEF DESCRIPTION OF THE DRAWINGS

[0055] Figure 1This is a schematic structural diagram of the electromagnetic wave sludge reduction community structure control system for improving microbial adhesion according to the present invention.

[0056] In the accompanying drawings, the components represented by the reference numerals are as follows:

[0057] 1. Anaerobic tank; 2. Anoxic tank; 3. Aerobic tank; 4. Secondary sedimentation tank; 5. First electromagnetic wave loading unit; 6. Second electromagnetic wave loading unit; 7. Original return sludge pipe. DETAILED DESCRIPTION

[0058] The principles and features of the present invention are described below with reference to the accompanying drawings. The examples given are only used to explain the present invention and are not used to limit the scope of the present invention.

[0059] Unless otherwise specified, the test methods in the examples are conventional methods; the materials and reagents used are commercially available unless otherwise specified.

[0060] The sewage water quality indicators are: COD: 240~250mg / L, TN: 35~40mg / L, TP: 3.5~4mg / L, pH: 6~8.

[0061] During the reactor startup phase, the return sludge from the Wuhan Longwangzui Wastewater Treatment Plant was used as inoculated sludge.

[0062] In a specific embodiment, Figure 1 As shown, the electromagnetic wave sludge reduction community structure control system for improving microbial adhesion includes: an A / A / O reactor, an inlet pipeline, a secondary sedimentation tank 4, a first electromagnetic wave loading unit 5, and a second electromagnetic wave loading unit 6. The A / A / O reactor includes an anaerobic tank 1, an anoxic tank 2, and an aerobic tank 3, which are connected in sequence.

[0063] The water inlet pipe connects to the anaerobic tank 1. The secondary sedimentation tank 4 connects to the aerobic tank 3, which has a sludge outlet. The first electromagnetic wave loading unit 5 has a first flow channel, with both ends of the first flow channel connected to the water inlet pipe and the sludge outlet respectively through sludge return pipes. The second electromagnetic wave loading unit 6 has a second flow channel, with both ends of the second flow channel connected to the anoxic tank 2 and the aerobic tank 3 respectively through the nitrification liquid return pipe.

[0064] The first electromagnetic wave loading unit 5 and the second electromagnetic wave loading unit 6 can respectively adopt the following existing technologies: a box-type electromagnetic wave generator is used, a flow channel is set through the box, and the flow channel material is a silicone tube. The through connection between the flow channel and the electromagnetic wave generator is sealed.

[0065] In order to further allow the return sludge and return nitrification liquid to flow at an appropriate speed, a peristaltic pump is provided between the first electromagnetic wave loading unit 5 and the water inlet pipeline, and a peristaltic pump is provided between the second electromagnetic wave loading unit 6 and the anoxic tank 2.

[0066] The mud outlet and the water inlet pipeline are connected through the original return sludge pipe 7.

[0067] In a specific embodiment, the electromagnetic wave sludge reduction community structure control method for improving microbial adhesion can be implemented and verified according to the following steps:

[0068] Step 1: Set up an electromagnetic wave sludge reduction community structure control system to improve microbial adhesion. In the A / A / O reactor, the volume ratio of the anaerobic zone, the anoxic zone, and the aerobic zone is 2.2:2.2:6.5.

[0069] Step 2: Based on the operating conditions of Step 1, three control experiments were designed. The first group did not apply electromagnetic waves, the second group applied electromagnetic waves to the external return sludge pipe, and the third group applied electromagnetic waves to both the external return sludge pipe and the internal nitrification liquid return pipe. All experimental groups maintained the same experimental conditions.

[0070] Step 3: Based on the sludge samples from each reaction tank obtained in Step 2, the sludge is subjected to performance analysis and high-throughput analysis. Sludge performance analysis includes analysis of contact angle, protein secondary structure, and surface roughness. High-throughput analysis primarily uses bioinformatics methods to annotate microbial functional genes, focusing on the effects of electromagnetic waves on the expression of genes related to microbial attachment (such as flagellin, pili genes, and related operational genes), and exploring the regulatory mechanisms of electromagnetic waves on microbial attachment behavior.

[0071] By regulating the electromagnetic wave loading conditions (loading power, loading time), the amount of returned sludge loaded is adjusted.

[0072] The reactors for the anaerobic, anoxic and aerobic parts are made of plexiglass to ensure sealing; water inlet pumps, outlet pipes and regulating valves are installed to ensure flow controllable; an aeration system is set up in the aerobic zone to adjust the gas flow; and the loading frequency of the electromagnetic wave loading unit is set.

[0073] After the system had been running stably for 30 days, a branch pipe of the electromagnetic wave loading device was added to the return sludge pipeline, connected to the sludge inlet and outlet pipes of the continuous flow 2450 MHz electromagnetic wave loading device, with a sampling port left. A peristaltic pump was added to the sludge inlet pipeline, and the flow rate was adjusted by adjusting the peristaltic pump speed.

[0074] During the test, the electromagnetic wave loading power is changed by adjusting the input voltage, the loading time is changed by adjusting the branch pipe length (for example, adjusting the straight pipe to a curved pipe to increase the path length), and the loading percentage is changed by adjusting the peristaltic pump speed.

[0075] Based on the electromagnetic wave loading conditions for sludge, a continuous 2450MHz electromagnetic wave loading circuit was installed on the reactor's nitrification liquid return line to load the internal nitrification liquid. System operation tests were conducted using electromagnetic wave loading for both internal and external nitrification liquid return in A / A / O systems. During the tests, loading conditions were varied by adjusting the input voltage, branch pipe length, and peristaltic pump speed.

[0076] Example

[0077] 1) The effective volumes of the anaerobic, anoxic, aerobic, and sedimentation tanks are 2.2 L, 2.2 L, 6.5 L, and 7.0 L, respectively. The hydraulic retention time is 6 hours, and the sludge age is 15 days. PVC pipes are used to connect the tanks, allowing for the natural flow of the mixed liquor by gravity.

[0078] 2) The aerobic tank is aerated with microporous aeration discs, and the dissolved oxygen concentration is controlled at 2.0 mg / L using a rotor flowmeter. The return of sludge and nitrification solution is controlled by a peristaltic pump, with an external sludge return ratio of 70% and an internal nitrification solution return ratio of 250%.

[0079] 3) Electromagnetic wave generator, operating frequency is 2450MHz.

[0080] The A / A / O system is operated by setting different electromagnetic wave loading parameters to achieve the sludge reduction effect. The specific information is as follows:

[0081] After 30 days of stable operation under operating conditions K0 (no electromagnetic wave loading), W1 (electromagnetic wave loading of return sludge: loading power = 275 W, loading time = 50 s, loading percentage = 25%), and X2 (electromagnetic wave loading of return sludge: loading power = 275 W, loading time = 50 s, loading percentage = 25%; electromagnetic wave loading of return nitrification solution: loading power = 420 W, loading time = 70 s, loading percentage = 15%), sludge was collected from the aerobic, anoxic, and anaerobic tanks for sludge performance analysis and metagenomic sequencing. To ensure the integrity of the experiment, the amount of residual sludge was also measured to analyze the sludge reduction effect.

[0082] The sludge properties of the anaerobic tank, anoxic tank, and aerobic tank under different electromagnetic wave loading conditions are shown in Table 1.

[0083] Table 1 Analysis of sludge properties under different electromagnetic wave loading conditions

[0084]

[0085] The data in Table 1 show that electromagnetic wave loading can significantly improve the surface properties of sludge. Contact angle results show that the hydrophobicity of each reaction tank has been significantly improved. Among them, the increase in the X2 working condition was the most significant, reaching 24.81%, 42.43% and 32.35% respectively. This increase in hydrophobicity promotes the interaction between microorganisms and is conducive to the formation of biological aggregates. Analysis of protein secondary structure found that electromagnetic wave treatment significantly reduced the ratio of α-helix to (β-sheet + random coil), indicating that after electromagnetic wave treatment, the protein structure tends to be loose. This change further enhances the hydrophobic properties of the sludge. In addition, atomic force microscopy observations show that the surface roughness of the sludge under the X2 working condition increased exponentially, increasing by 197%, 108% and 280% respectively compared with K0, providing a more favorable interface environment for microbial attachment.

[0086] The abundance of flagellar synthesis genes in the anaerobic, anoxic, and aerobic tanks under different electromagnetic wave loading conditions is shown in Table 2.

[0087] Table 2 Abundance of flagellar synthesis genes under different electromagnetic wave loading conditions

[0088]

[0089] The data in Table 2 indicate that electromagnetic wave treatment significantly inhibited flagellar motility and the expression of motility-related genes in microorganisms in sludge. Under the W1 condition, the abundance of flagellar motility-related genes in the anaerobic, anoxic, and aerobic tanks decreased by 9.36% compared to the K0 condition. Under the X2 condition, the decreased gene abundance further increased to 27.35%, indicating that electromagnetic wave treatment effectively reduced microbial motility and made it easier for microorganisms to adhere to the sludge surface.

[0090] The abundance of fimbriae synthesis proteins in anaerobic, anoxic, and aerobic tanks under different electromagnetic wave loading conditions is shown in Table 3.

[0091] Table 3 Abundance of pili synthesis proteins under different electromagnetic wave loading conditions

[0092]

[0093] The data in Table 3 show that under the W1 operating condition, the abundance of fimbriae synthesis proteins in the anaerobic, anoxic, and aerobic tanks decreased by 6.26% compared to the K0 operating condition, indicating that electromagnetic wave loading of the external return sludge inhibited the expression of fimbriae synthesis proteins. Studies have shown that the expression of fimbriae synthesis proteins is positively correlated with microbial attachment ability. This indicates that the effect of electromagnetic wave loading only on the external return sludge pipeline on improving microbial attachment performance has certain limitations. Under the X2 operating condition, the abundance of fimbriae synthesis proteins in the anaerobic, anoxic, and aerobic tanks increased significantly by 13.2% compared to the K0 operating condition. This indicates that when electromagnetic waves are synergistically loaded on the internal return nitrification solution and the external return sludge, they can effectively promote the expression of fimbriae synthesis proteins, thereby promoting interactions between microorganisms and improving adhesion.

[0094] To ensure the integrity of the experiment, the apparent sludge yield in the A / A / O system was used to analyze the sludge reduction effects under different operating conditions. For example, the mass of suspended solids produced per gram of COD was calculated based on the sum of the sludge concentrations in the anaerobic, anoxic, and aerobic tanks. The experimental results showed that under the K0 operating condition, without electromagnetic wave application, the apparent sludge yield (Yobs) was 0.2158 g MLSS / g COD. When operating under the W1 operating condition, the apparent sludge yield (Yobs) dropped to 0.1532 g MLSS / g COD, achieving a 29.00% sludge reduction compared to the K0 condition. The further optimized X2 operating condition achieved even more significant reduction results, with a Yobs of 0.1366 g MLSS / g COD and a 36.70% sludge reduction rate, a 7.70 percentage point increase compared to the W1 condition.

[0095] The experimental results show that the coordinated loading of electromagnetic waves on internal and external return sludge can effectively enhance the adhesion of microorganisms and promote sludge reduction. The main principle is: electromagnetic wave loading provides more attachment sites for microorganisms by increasing the surface properties and structure of sludge, making it easier for microorganisms to accumulate on the sludge surface. By regulating the expression of motility genes in functional bacterial communities, the initial attachment on the sludge surface is converted to irreversible attachment, thereby achieving targeted regulation of specific functional bacterial communities.

[0096] This invention enhances the attachment properties of microorganisms by loading them with electromagnetic waves, improving sludge reduction and alleviating the high energy consumption and potential secondary pollution issues inherent in traditional sludge treatment. Experimental results show that synergistic electromagnetic wave loading can increase sludge attachment sites, simultaneously downregulate flagellar gene expression and upregulate fimbriae gene expression, enhancing irreversible microbial attachment and achieving targeted regulation of specific functional bacterial communities, contributing to the advancement of sludge reduction technology.

[0097] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. An electromagnetic wave sludge reduction community structure control system for improving microbial adhesion, characterized by: include: An A / A / O reactor comprises an anaerobic tank (1), an anoxic tank (2) and an aerobic tank (3) which are connected in sequence; A water inlet pipe connected to the anaerobic tank (1); A secondary sedimentation tank (4) connected to the aerobic tank (3), wherein the secondary sedimentation tank (4) has a mud outlet; A first electromagnetic wave loading unit (5) having a first flow channel, wherein both ends of the first flow channel are connected to the water inlet pipeline and the mud outlet via a sludge return pipe; A second electromagnetic wave loading unit (6) has a second flow channel, with both ends of the second flow channel connected to the anoxic tank (2) and the aerobic tank (3) via a nitrification liquid return pipeline; and an original return sludge pipe (7) communicating with the sludge outlet and the water inlet pipeline.

2. The electromagnetic wave sludge reduction community structure control system for improving microbial adhesion according to claim 1 is characterized by: A microporous aeration disk is provided in the aerobic tank (3), and the microporous aeration disk is connected to an air pump.

3. The electromagnetic wave sludge reduction community structure control system for improving microbial adhesion according to claim 1 is characterized by: A peristaltic pump is provided in communication between the first electromagnetic wave loading unit (5) and the water inlet pipeline.

4. The electromagnetic wave sludge reduction community structure control system for improving microbial adhesion according to claim 1 is characterized by: A peristaltic pump is provided in communication between the second electromagnetic wave loading unit (6) and the anoxic pool (2).

5. A method for regulating the community structure of sludge reduction by electromagnetic waves to enhance microbial adhesion, characterized in that: The electromagnetic wave sludge reduction community structure control system for improving microbial adhesion according to any one of claims 1 to 4 comprises the following steps: 1) Use A / A / O reactor to treat sewage and operate stably for a period of time; 2) A portion of the sludge from the secondary sedimentation tank is introduced as return sludge into the anaerobic tank. The return sludge is continuously excited by electromagnetic waves to improve the surface properties of the sludge in the anaerobic, anoxic, and aerobic tanks, and to reduce the abundance of flagellar synthesis genes; 3) Part of the nitrification liquid from the aerobic tank is passed into the anoxic tank as return nitrification liquid. The return nitrification liquid is continuously excited by electromagnetic waves to improve the surface properties of the sludge in the anaerobic tank, anoxic tank, and aerobic tank, and to reduce the abundance of flagellar synthesis genes and increase the abundance of fimbriae synthesis genes.

6. The electromagnetic wave sludge reduction community structure control method for improving microbial adhesion according to claim 5, characterized in that: The power of continuous electromagnetic wave excitation for return sludge is 265-285 W; The continuous excitation time of electromagnetic waves on the return sludge is 45-55 s; Return sludge accounts for 20-30% of the total sludge; The frequency of continuous excitation of electromagnetic waves for return sludge is 2400-2500 MHz.

7. The electromagnetic wave sludge reduction community structure control method for improving microbial adhesion according to claim 5, characterized in that: The power of continuous electromagnetic excitation of the reflux nitrification solution is 410-430 W; The time of continuous excitation of electromagnetic waves on the reflux nitrification solution is 65-75 s; The reflux nitrification liquid accounts for 10-20% of the total nitrification liquid; The frequency of the continuous excitation of electromagnetic waves on the reflux nitrification liquid is 2400-2500 MHz.

8. The electromagnetic wave sludge reduction community structure control method for improving microbial adhesion according to claim 5, characterized in that: After the A / A / O reactor has been running stably for 25-35 days, continuous electromagnetic wave excitation is performed; Working temperature is 20-30 ℃; The dissolved oxygen in the aerobic tank is 1.90-2.10 mg / L; The sludge return ratio is 65-75%; The reflux ratio of nitrification liquid is 240-260%; The sludge retention time is 14-16 days.

9. The electromagnetic wave sludge reduction community structure control method for improving microbial adhesion according to claim 5, characterized in that: The surface properties of the sludge include any one or more of a contact angle, an α-helix / (β-sheet+random coil) value, or a surface roughness.

Images