Test method for multi-item joint inspection through single-time sample adding

Abstract

The invention relates to the technical field of micro-fluidic POCT (point-of-care testing) detection, and particularly discloses a testing method for multi-item joint detection by single sample adding. Wherein the test disc is provided with a test liquid accommodating cavity, a liquid guide groove, a liquid storage groove, an air hole, an overflow port, an overflow liquid collecting groove, a flat pressing hole, a drainage groove, a test liquid outlet and a test paper mounting groove; according to the test disc, the test liquid is accurately shunted to a plurality of test paper mounting grooves through the test liquid accommodating cavity, the liquid guide groove and the liquid storage groove by utilizing a micro-fluidic technology under the action of centrifugal force, so that multi-index high-sensitivity detection is realized, the problem of low efficiency of one-by-one operation in a traditional method is solved, and the high-throughput detection requirement is met.

Description

Technical Field

[0001] The present invention relates to the field of microfluidic POCT detection technology, and in particular to a testing method for single-sample multi-item joint detection. Background Art

[0002] Existing test strip detection technology usually uses manual drop-by-drop addition and manual visual observation of the results. During the test, the operator adds the sample solution or reagent to be tested drop by drop onto the test strip and judges the test result by observing the color change of the indicated area or the appearance of the marked line on the test strip. This test strip-based detection method is simple and intuitive to operate and has been widely used in medical diagnosis, environmental monitoring and other fields. However, the traditional test strip detection method has obvious limitations and shortcomings. The current test strip detection method mainly has the following problems:

[0003] Traditional methods often only test a single test strip at a time. Testing multiple samples requires individual testing, a cumbersome and inefficient process that fails to meet the demands of high-throughput, rapid batch testing. For example, when analyzing multiple samples simultaneously, operation time increases significantly, limiting its effectiveness in modern laboratories or industrial applications. Manual drop-by-drop additions struggle to precisely control the amount of solution added each time. To ensure adequate reaction, excess test solution is often added, resulting in some unreacted and wasted. Furthermore, manual additions are prone to overflow or residue, further reducing the effective utilization of test solution. Due to differences in operator technique and judgment, test results can vary between batches or between different individuals. The color development process of test strips is significantly affected by human factors, such as operating force and dosing time, making it difficult to obtain consistent results across repeated tests. Test strip results often rely on visual comparison of color or markings, which can be subject to significant subjective error. Furthermore, external factors such as ambient light and viewing angle can affect interpretation accuracy. The lack of standardized reaction control and reading methods makes it difficult to ensure test accuracy and reliability.

[0004] To overcome these limitations, several automated systems have been developed. For example, urine analyzers can automatically read urine test strips, providing digital results for multiple parameters such as pH, protein, glucose, and ketone bodies. Blood glucose test strip readers can also quickly and accurately provide blood glucose concentrations. These automated devices analyze test strip reactions using optical or electrochemical methods, significantly improving the efficiency and accuracy of single-strip testing. However, these devices are typically designed only for single-strip testing and struggle to scale up multiple test strips. For high-throughput applications requiring simultaneous testing of multiple test strips, existing technologies lack comprehensive solutions. Furthermore, while multi-well plates used in laboratories can be used for batch processing of liquid samples, their design and processing methods are not directly applicable to test strips, as the physical form and reaction mechanisms of test strips differ from those of liquid samples. While some devices for processing multiple test strips exist on the market, such as test strip dispensers and cutters, these devices are primarily designed for dispensing single test strips or cutting test strips from large sheets, rather than for simultaneous testing of multiple test strips.

[0005] Therefore, the existing technology lacks a structured testing method that can simultaneously address the challenges of batch testing, efficient test solution utilization, consistent results, and guaranteed test accuracy. In other words, there is currently a lack of a dedicated device that integrates and optimizes the test strip testing process and overcomes the aforementioned deficiencies through a specific structural design. Therefore, there is a need for a device that can implement a batch testing method for chemical test strips that supports simultaneous, quantitative, and high-throughput analysis, ensures uniform application of the test solution, and provides accurate and consistent test results. Summary of the Invention

[0006] The purpose of the present invention is to solve the shortcomings of the prior art. In order to achieve the above purpose, the present invention adopts the following technical solutions:

[0007] The present invention provides a method for testing multiple items in a single sample addition. The method uses a test disc connected to the top of an external rotating base, which is used to drive the test disc to rotate. The test disc is provided with: a test liquid accommodating chamber, a liquid guide groove, a liquid storage groove, an air hole, an overflow port, an overflow liquid collection groove, a pressure-equalizing hole, a drainage groove, a test liquid outlet, and a test paper installation groove;

[0008] Among them, the test liquid accommodating chamber is located in the test tray; the liquid guide groove, one end of which is connected to the test liquid accommodating chamber and the other end is connected to the liquid storage tank; the air hole is connected to the liquid storage tank; the overflow port is arranged at the upper part of the liquid storage tank and is connected to the overflow liquid collection tank; the pressure equalization hole is arranged on the overflow liquid collection tank; the drainage groove, one end of which is connected to the bottom of the liquid storage tank and the other end is connected to the test liquid outlet; the test paper installation groove is arranged on the test tray; the test liquid outlet is arranged on the test tray and located above the test paper installation groove;

[0009] The test method includes the following steps:

[0010] Add the test liquid into the test liquid receiving cavity of the test tray;

[0011] The test disc is driven to rotate by an external rotating seat, so that the test liquid flows through the liquid guide groove into the liquid storage tank under the action of centrifugal force;

[0012] When the liquid storage tank and the air hole are filled with the test liquid, the excess test liquid is discharged into the overflow liquid collection tank through the overflow port, and the air pressure is balanced through the pressure equalization hole;

[0013] Under the siphon effect of the drainage trough, the test liquid in the storage tank is led to the test liquid outlet;

[0014] The test liquid is dripped onto the test paper in the test paper installation groove through the test liquid outlet, so that the test paper reacts with the test liquid.

[0015] Preferably, the test liquid accommodating cavity is a circular or polygonal groove located in the center of the test disc, and a side wall thereof is provided with an opening connected to the liquid guiding groove.

[0016] Preferably, the drainage groove is a curved channel.

[0017] Preferably, a mounting portion is provided at the center of the bottom of the test disc, and the mounting portion is mounted on the external rotating base and is detachably connected to the external rotating base.

[0018] Preferably, one or more groups of test paper fixing ribs are provided in the test paper installation groove, and the test paper fixing ribs are used to fix the test paper installed in the test paper installation groove.

[0019] Preferably, the test paper installation slots are evenly distributed around the periphery of the test disc.

[0020] Preferably, an annular diversion wall is provided on the periphery of the test liquid accommodating chamber, and the diversion wall divides the test liquid accommodating chamber into a plurality of flow channels, each of which is connected to a corresponding liquid storage tank through a liquid guide groove.

[0021] Preferably, the overflow liquid collecting groove is an annular groove, which is arranged around the periphery of the test disc.

[0022] Preferably, the drainage trough is arranged at a height higher than the liquid storage tank.

[0023] Beneficial effects of the present invention: The present invention provides a test method for single-sample multi-item joint testing. By connecting to an external rotating seat, the rotation mechanism is used to achieve uniform distribution of the test liquid and synchronous detection of multiple test strips, which significantly overcomes many shortcomings in the prior art. First, the test disc uses the structural design of the test liquid accommodating cavity, the liquid guide groove and the liquid storage tank to accurately divert the test liquid to multiple test paper installation grooves under the action of centrifugal force, thereby realizing batch testing, solving the problem of low efficiency of the traditional method of one-by-one operation, and meeting the needs of high-throughput testing. Secondly, the liquid storage tank is combined with the air hole and the overflow port to ensure the quantitative distribution of the test liquid, and the excess liquid is recovered through the overflow liquid collection tank, thereby improving the utilization rate of the test liquid and reducing waste. The equalization hole further balances the air pressure to ensure the stability of the liquid flow. In addition, the drainage trough uses the siphon effect to evenly add the test liquid to the test paper, avoiding the deviation of manual liquid addition and significantly improving the repeatability and consistency of the test results. At the same time, the automated distribution and reaction process reduces human operation and subjective interpretation errors, and improves the detection accuracy and reliability. The present invention has a simple structure and is easy to operate, is applicable to the fields of medical diagnosis, environmental monitoring, etc., and can efficiently and quantitatively complete the synchronous detection of multiple test strips. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Various other advantages and benefits will become apparent to those skilled in the art upon reading the detailed description of the preferred embodiment below. The accompanying drawings are for illustration purposes only and are not to be considered as limiting the present invention. The same reference numerals are used throughout the accompanying drawings to denote the same components. In the accompanying drawings:

[0025] Figure 1 is a three-dimensional diagram of a reagent disc provided in an embodiment of the present invention;

[0026] Figure 2 is a cross-sectional view of a reagent disc provided in an embodiment of the present invention;

[0027] Figure 3 This is a front view of a reagent disc provided by an embodiment of the present invention;

[0028] Figure 4 It is a rear view of the reagent tray provided in an embodiment of the present invention.

[0029] Icons: 1-test liquid holding chamber; 2-liquid guide groove; 3-liquid storage groove; 4-air hole; 5-overflow outlet; 6-overflow liquid collection groove; 7-equalizing hole; 8-drainage groove; 9-test liquid outlet; 10-test paper installation groove; 11-diversion wall; 12-installation part; 13-test paper fixing rib. DETAILED DESCRIPTION

[0030] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the present invention will be briefly introduced below in conjunction with the drawings and the description of the embodiments or the prior art. Obviously, the following description of the structures of the drawings is only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative work. It should be noted that the description of these embodiments is used to help understand the present invention, but does not constitute a limitation of the present invention.

[0031] It should be noted that similar reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined in one drawing, it does not need to be further defined or explained in subsequent drawings.

[0032] Example 1:

[0033] like Figures 1 to 4 As shown, a test method for single-sample multi-item joint testing proposed in an embodiment of the present invention is used in connection with an external rotating seat, such as being connected to the external rotating seat through a mounting portion 12, and realizing quantitative distribution of the test liquid and batch test paper testing by rotation. The test disc in this embodiment includes: a test liquid accommodating chamber 1 arranged in the center of the disc body, a plurality of liquid guide grooves 2 radially extending from the test liquid accommodating chamber 1 to the periphery of the disc body, a liquid storage tank 3 located at the end of each liquid guide groove 2, and a plurality of test paper mounting grooves 10 for mounting test paper. In addition, an auxiliary structure that cooperates with the liquid circuit is also provided on the test disc, including an air hole 4, an overflow port 5, an overflow liquid collecting tank 6, a pressure-equalizing hole 7, a drainage tank 8 and a test liquid outlet 9, to ensure the smoothness of the test liquid distribution process and the reliability of the detection.

[0034] The test liquid holding chamber 1 is located at the center of the test disc. It is a cavity recessed into the disc body and is used to hold the test liquid to be tested. Preferably, the test liquid holding chamber 1 is a circular or polygonal groove located in the center of the test disc, and its side wall is provided with an opening connected to the liquid guide groove for distributing the test liquid; at the same time, the holding chamber 1 can be a circular or nearly circular groove structure, with its opening facing upward to facilitate the injection of liquid from above. When the test disc is mounted on the rotating seat, the test liquid holding chamber 1 is coaxial with the axis of rotation, thereby generating a uniform centrifugal force field during rotation. Preferably, the volume of the test liquid holding chamber 1 is designed according to the sample volume required for a single test, and can accommodate the entire test liquid volume required for a single test.

[0035] A plurality of liquid guide grooves 2 extend radially outward from the test liquid accommodating chamber 1. The liquid guide grooves 2 are elongated channel structures provided on the disc body, and are used to guide the test liquid from the central accommodating chamber to the periphery of the disc body. A plurality of liquid guide grooves 2 are evenly distributed on the disc surface. For example, in this embodiment, the number of liquid guide grooves 2 is 6, and they are arranged at 60° intervals around the center. One end of each liquid guide groove 2 is connected to the test liquid accommodating chamber 1, and the other end is connected to the corresponding liquid storage tank 3. The liquid guide grooves 2 are usually formed by a groove formed on the disc body and a cover plate above it to form a closed pipe. When the test disc rotates, the test liquid moves rapidly along the liquid guide grooves 2 under the action of centrifugal force, thereby realizing transmission from the center to the outside.

[0036] At the connection between the test liquid accommodating chamber 1 and each liquid conducting groove 2, a plurality of radially arranged diverter walls 11 are provided. The diverter wall 11 is a vertical partition structure, extending outward from the inner wall of the accommodating chamber 1, separating the adjacent liquid conducting groove 2 entrances from each other. Through these diverter walls 11, the test liquid accommodating chamber 1 is divided into a plurality of outlet areas, and each liquid conducting groove 2 has a liquid outlet channel. Such a design plays a diverting role when the test disk rotates, so that the test liquid can be distributed approximately evenly into each liquid conducting groove 2, avoiding the liquid from flowing to individual channels. In this embodiment, the diverter walls 11 correspond one-to-one to the positions of the liquid conducting grooves 2. For example, if there are 6 liquid conducting grooves 2, 6 diverter walls 11 are arranged to divide the liquid outlet of the accommodating chamber 1 into 6 equal areas.

[0037] The outer end of the liquid guide groove 2 is connected to a liquid storage tank 3. The liquid storage tank 3 is located near the periphery of the test disk. It is an expanded cavity at the end of the liquid guide groove 2 and is used to temporarily store the test liquid transported from the liquid guide groove 2. Each liquid guide groove 2 corresponds to a liquid storage tank 3, and the number and layout of the liquid storage tanks 3 are consistent with the liquid guide groove 2 (such as 6 in this embodiment, evenly distributed around the disk body). The volume of the liquid storage tank 3 is designed to accommodate the amount of liquid required for a test strip to be tested. The shape of the liquid storage tank 3 can be a circular, elliptical or other suitable geometric shape of a pit, and its depth is generally greater than the liquid guide groove 2, so that a certain amount of liquid can be retained even after the rotation stops. The setting of the liquid storage tank 3 provides a gathering and stable space for the test liquid before it reaches the test strip, which is conducive to quantitative distribution: when each liquid storage tank 3 is filled, it means that the corresponding test strip has obtained a sufficient and approximately the same amount of sample liquid.

[0038] To prevent bubbles from forming during high-speed liquid flow or dripping, which could affect proper distribution, an air hole 4 is provided above each reservoir 3. The air hole 4 is typically a small space located on the periphery of the reservoir 3. This space is connected to the outside atmosphere via the drainage groove 8 and the test liquid outlet 9, and is also connected to the interior of the reservoir 3. Its function is to expel any remaining air within the reservoir 3 before the liquid fills the reservoir 3, preventing air entrapment and the formation of bubbles. The air holes 4 ensure that the liquid can smoothly fill the entire space of the reservoir 3, preventing complete filling due to air blockage. These small holes can be relatively small, primarily to help eliminate bubbles between the reservoir 3 and the drainage groove 8. When the test liquid enters the reservoir 3, centrifugal force first forces the test liquid to rapidly enter the air hole 4, allowing the test liquid to quickly accumulate near the air hole 4 in the reservoir 3, thereby rapidly filling the reservoir 3. Preferably, the drainage groove 8 is a curved channel suitable for guiding the test liquid from the reservoir 3 to the test liquid outlet 9 via a siphon effect.

[0039] In order to ensure the quantitative amount of liquid in each liquid reservoir 3 and prevent excessive overflow, each liquid reservoir 3 is also provided with an overflow port 5. The overflow port 5 is an outlet channel opened on the side wall or top of the liquid reservoir 3, which is used to guide excess liquid out of the tank when the liquid exceeds a specific height or capacity. The overflow port 5 is usually located at the highest height allowed by the liquid reservoir 3. When the test liquid continues to flow into the liquid reservoir 3 through the liquid guide groove 2, once the liquid level in a certain liquid reservoir 3 reaches its designed capacity, the excess liquid will flow out of the overflow port 5 of the liquid reservoir 3. In order to collect this excess liquid, the test tray is provided with an overflow liquid collection tank 6 connected to all overflow ports 5.

[0040] Preferably, the overflow liquid collection tank 6 is an annular tank, which is arranged around the periphery of the test disc and is used to collect the test liquid overflowing from each liquid storage tank 3 through the corresponding overflow port. The overflow liquid collection tank 6 is generally formed around the periphery of the test disc and is an annular groove or cavity structure, which is used to collect and store excess test liquid discharged from each overflow port 5. The collection tank 6 is usually located at the outer periphery of all liquid storage tanks 3, and the centrifugal force during rotation can be used to throw the overflowed liquid into the collection tank. Through the overflow liquid collection tank 6, the excess sample liquid is effectively isolated on the outer ring of the disc body to prevent it from flowing back or contaminating the test paper area. In addition, the collection tank 6 can also serve as a safe storage, which is convenient for cleaning up these residual liquids after the test is completed.

[0041] A pressure-equalizing hole 7 is also provided at an appropriate position of the test disc, and its function is to balance the air pressure between the liquid circuit system in the disc body and the external environment. Since the test disc will experience rapid liquid flow and space filling during the liquid transportation and distribution process, if there is not enough ventilation, positive or negative pressure may be formed locally, affecting the normal flow of the liquid. The pressure-equalizing hole 7 is usually provided above the test liquid accommodating cavity 1 or on the disc cover, and is connected to the internal space of the accommodating cavity or the liquid guide groove 2. Through the pressure-equalizing hole 7, atmospheric pressure can enter the key nodes of the liquid circuit system, thereby avoiding uneven or stopped liquid flow caused by changes in air pressure in the enclosed space. It should be noted that the air hole 4 also has the function of balancing air pressure to a certain extent, but the pressure-equalizing hole 7 is mainly to allow the test liquid in the liquid storage tank 3 to flow smoothly into the overflow liquid collection tank 6.

[0042] Each liquid reservoir 3 is provided with a test liquid outlet 9 at the bottom or lower side. The test liquid outlet 9 is typically a small hole or slit that leads from the liquid reservoir 3 to the test paper mounting slot 10 below. Multiple test paper mounting slots 10 are evenly distributed around the perimeter of the test disc. The function of the test liquid outlet 9 is to direct liquid dripping onto the test paper after the liquid reservoir 3 is full. As the test disc rotates, centrifugal force fills the liquid reservoir 3 with liquid, after which the test liquid flows through the drainage slot 8 into the outlet 9 and out in the form of drops or a thin stream. The size and shape of the test liquid outlet 9 can be designed as needed to control the dripping rate and flow rate, ensuring that the liquid wets the test paper without excessively flooding it. Typically, the diameter of the outlet 9 is relatively small, providing a certain degree of throttling, allowing the liquid to flow slowly, drop by drop, onto the test paper surface. In this embodiment, to guide the liquid smoothly from the test liquid outlet 9 to the test paper and prevent it from overflowing, a drainage slot 8 is also provided near each test paper mounting slot 10. The drainage trough 8 can be understood as a small groove or diversion structure extending from the test liquid outlet 9, which is used to guide the dripping liquid to accurately land on the target area of ​​the test paper and to drain the remaining liquid away after the test paper is soaked. Specifically, the drainage trough 8 is usually located downstream of the test paper mounting groove 10, tangent to or slightly lower than the surface of the test paper. Preferably, when the liquid drops onto the test paper and the excess flows along the test paper, the drainage trough 8 can collect the liquid not absorbed by the test paper and divert it to the overflow liquid collection trough 6. In this way, after the test paper is fully wetted, the excess liquid will not be retained on the test paper surface or in the trough, but will be promptly discharged to the peripheral collection trough 6, thereby keeping the test paper reaction area environment relatively clean and facilitating subsequent result reading. Preferably, the drainage trough 8 is arranged at a height higher than the liquid reservoir 3 so that when the liquid reservoir is filled with test liquid, the drainage trough 8 generates a siphon effect to divert the test liquid in the liquid reservoir 3 to the test liquid outlet.

[0043] The test strip mounting slots 10 are structures for placing and securing test strips. In this embodiment, six test strip mounting slots 10 are evenly distributed on the test disc, corresponding one-to-one to the six liquid guide slots 2 and liquid reservoirs 3. The test strip mounting slots 10 are generally elongated, narrow slots, slightly larger than the length and thickness of a standard test strip, allowing the test strip to lie flat or fit snugly within the slots. The position and orientation of each mounting slot 10 are designed so that when a test strip is placed within it, its sensing area (e.g., the multiple reagent reaction pads on a urine test strip) is positioned directly below or near the corresponding test liquid outlet 9. This way, any liquid flowing from outlet 9 directly contacts the test strip's reaction area. The test strip can be secured within the mounting slots 10 using snaps, elastic pressure plates, or other securing structures to prevent displacement due to centrifugal force during rotation. Preferably, one or more test strip securing ribs 13 are provided within the test strip mounting slots 10 to secure the test strip within the mounting slots 10. Typically, these test strip slots are arranged radially around the center of the disc, with one end of each test strip facing the central chamber 1 and the other end toward the periphery of the disc. Once the test strip is installed, its liquid-receiving end faces inward, aligned with the test liquid outlet 9, ensuring that drops of liquid land accurately on the test strip. By providing multiple test strip mounting slots 10 on the test disc, multiple test strips can be tested simultaneously using the same sample, significantly improving testing efficiency.

[0044] Usage scenarios and usage process: This test disc is mainly used in clinical urine test strips and other situations where multiple test strips need to be processed at once. The following describes the specific steps for using the test disc of the present invention in conjunction with the detection process of routine urine analysis test strips.

[0045] First, the operator selects several urine test strips based on the test item being tested and inserts them into the test strip mounting slots 10 of the test tray. During insertion, ensure that the sensing end of each test strip (i.e., the end with the reagent pad) faces the center of the tray and aligns with its corresponding test fluid outlet 9. The test strips can be secured firmly within the slots by gently pressing or snapping. At this point, all test strips are in place, with each test strip in each slot on the tray ready to receive the sample fluid.

[0046] Next, prepare the urine sample to be tested as the test liquid. Keep the test plate horizontal and slowly pour the urine into the test liquid holding chamber 1 in the center. The amount of urine injected needs to be appropriate, usually slightly more than the total amount required for all test strips to ensure that each reservoir 3 can be filled. For example, if each test strip requires approximately 0.2mL of urine, and 6 test strips require a total of about 1.2mL, then about 1.5mL of urine can be added to the holding chamber 1 to reserve a certain margin. When adding the sample, the action should be as smooth as possible to avoid bubbles; the opening of the holding chamber 1 is large to facilitate pouring, and its groove-shaped structure allows the liquid to naturally gather at the bottom of the cavity, ready for subsequent distribution.

[0047] Next, install the test disc containing the test strip and sample liquid onto the external rotating base. Typically, the rotating base is a rotatable platform within the detection device, with a fixed structure that mates with the test disc to securely lock the disc. During installation, align the center hole or bottom positioning structure of the test disc mounting portion 12 with the rotating base's axis to ensure the disc is level and securely fixed to the rotating base. After confirming proper installation, activate the rotary drive mechanism, causing the test disc to begin accelerating around its central axis.

[0048] When the test disc accelerates, the urine in the accommodating cavity 1 is thrown to the periphery under the action of centrifugal force and enters the radially distributed liquid guide grooves 2. Due to the guiding effect of the diversion wall 11, the urine is divided into multiple streams and flows to different liquid guide grooves 2 at the same time. The urine moves rapidly along the liquid guide grooves 2, reaches the end of each liquid guide groove 2 almost at the same time and enters the corresponding liquid storage tank 3. The liquid is first collected in the liquid storage tank 3. When a liquid storage tank 3 is filled to its upper limit, the excess urine is discharged through the overflow port 5 and enters the peripheral overflow liquid collection tank 6. The other liquid storage tanks 3 are also filled in the same way. During this process, the air holes 4 continue to play a role, and the air in the tank is discharged in time to avoid interruption of the liquid flow. With the help of the above-mentioned structural design, the present invention can ensure that multiple liquid storage tanks 3 obtain sample liquid almost simultaneously and in equal amounts, and realize quantitative synchronous sampling of multiple test strips.

[0049] As the liquid reservoir 3 is gradually filled, the urine sample begins to drip downward onto the test paper through the test liquid outlet 9. When the liquid level in the liquid reservoir 3 rises to the position of the outlet hole, under the combined action of centrifugal force and gravity, the droplets drip successively onto the sensing area of ​​the test paper below through the small holes. As the test disc continues to rotate, each liquid reservoir 3 is stably aligned with the test paper below it, continuously supplying droplets. Basically, within a few seconds, the sensing area of ​​each test paper is completely wetted by urine. At this time, the color reaction of the corresponding test item begins to appear on each test strip. For example, the reagent pads such as glucose, protein, nitrite, etc. on the urine test paper undergo chemical reactions and change color after encountering urine.

[0050] Keep the test disc rotating for a set period of time (such as continuing to rotate for about 10 seconds) to ensure that all test papers are fully exposed to the sample liquid and their respective reactions are started evenly. Then stop the rotation drive and let the test disc gradually slow down until it stops. During the process of stopping the rotation, the urine flowing out through the test liquid outlet 9 also decreases and stops. At this time, the sample liquid supply on each test paper is sufficient and balanced. If there is still excess urine residue, it will be guided to the overflow liquid collection tank 6 through the drainage groove 8 before and after the rotation stops, and will not be retained on the surface of the test paper. After the test disc stops, start timing to allow the chemical reaction on the test paper to fully proceed (general urine routine test paper needs to react for 1 to 2 minutes).

[0051] While waiting for the reaction, you can observe that each test strip gradually presents blocks of different shades of color, indicating the preliminary results of each test parameter. Once the reaction time is up, the tester can read and analyze the color change of the test strip. Since all test strips start to react almost at the same time and obtain a similar amount of sample liquid, their color development results are synchronized and comparable, greatly improving the accuracy and reliability of the readings. When reading the results, the operator can remove the test disc from the rotating seat, take out the test strips one by one, and compare them with the standard colorimetric card to determine the concentration of each indicator; or in some automated devices, it can be read directly together with the test disc (for example, using an optical sensor to scan the color of each test strip in turn). Regardless of the reading method used, the use of this test disc avoids the tedious operation of manually adding samples one by one, making the detection of multiple test strips simple and efficient.

[0052] After the reading is complete, the test strip and test disc can be processed. The operator can remove the used test strip from the test strip mounting slot 10 and discard it. If the test disc is a disposable consumable, the entire disc can be discarded to avoid cross contamination. If the test disc is designed to be cleaned and reused, it should be removed from the rotating base, any remaining liquid in the overflow collection slot 6 should be discarded, and the disc should be thoroughly cleaned and dried before the next use.

[0053] Example 2:

[0054] The structure of the test tray provided in this embodiment is substantially similar to that of embodiment 1, except that the number of the test paper mounting slots 10 is increased and the fluid distribution structure is optimized.

[0055] Specifically, the test disc in Example 2 can accommodate more test strips to meet the needs of batch testing. The number of evenly distributed test strip mounting slots 10 on the disc body has increased from 6 in Example 1 to 12, with the center angle between each two adjacent test strip slots being approximately 30°, arranged in a circular array. Correspondingly, 12 liquid guide grooves 2 and 12 liquid storage grooves 3 are provided, each corresponding to the 12 test strip slots. This means that this embodiment can simultaneously accommodate twelve test strips for parallel testing, further improving the throughput of a single test.

[0056] To ensure uniform sample liquid distribution across all channels even when the number of liquid-conducting grooves 2 and liquid-reservoir grooves 3 doubles, Example 2 improves the central diversion structure. First, throttling slits or flow-limiting holes are provided at the interface between the test liquid-receiving chamber 1 and the liquid-conducting grooves 2. These slits act as flow restrictors. When the disk rotates, the flow-limiting holes at the entrance of each liquid-conducting groove 2 create a certain resistance to liquid flow, allowing the sample liquid to enter each liquid-conducting groove 2 at a nearly equal rate. In this way, even with machining errors or differences in liquid viscosity, the amount of liquid received by each channel tends to be consistent.

[0057] On the other hand, an annular distribution chamber is added between the accommodating chamber 1 and each liquid-conducting groove 2. This annular distribution chamber is arranged around the accommodating chamber 1 and is connected to the inlets of all liquid-conducting grooves 2. During rotation, the sample liquid first enters the annular distribution chamber from the accommodating chamber 1 and is then simultaneously diverted into multiple liquid-conducting grooves 2. Because the annular chamber temporarily stores and circumferentially balances the liquid, the synchronization of liquid distribution can be further improved, preventing certain channels from being overfilled due to being the first to contact the liquid. With this optimized structure, even if the number of test strip slots is significantly increased, the volume of sample liquid obtained by each test strip remains basically the same.

[0058] With the adjustments to the flow diversion and liquid-guiding structures, the number of diverter walls 11 in Example 2 has been increased accordingly, and an improved layout has been adopted. The new diverter walls 11 are approximately the same height as the accommodating chamber 1, but their thickness can be slightly reduced to minimize central volume occupation while providing multi-channel isolation. These walls, in conjunction with the aforementioned flow-restricting orifices and annular distribution chamber, ensure precise and synchronized liquid distribution during rotation.

[0059] In addition, Example 2 also strengthens the liquid storage tank 3 and its related supporting structures. As the number of test strips increases, the total sample usage increases accordingly, so the volume design of each liquid storage tank 3 is slightly increased to accommodate sufficient liquid. The shape of the liquid storage tank 3 can be optimized to be more conducive to the rapid and stable accumulation of liquid near the outlet, for example, the bottom is slightly conical and points to the test liquid outlet 9, so that the liquid can drip smoothly after the rotation stops. The diameter and opening height of the overflow port 5 on each liquid storage tank 3 have also been recalculated and adjusted to meet the overflow requirements of larger amounts of liquid, ensuring that when the twelve liquid storage tanks 3 are filled, the excess liquid can be discharged in time without retention. The overflow liquid collection tank 6 is correspondingly widened and deepened to have sufficient capacity to accommodate excess sample liquid discharged from all channels.

[0060] In terms of ventilation and balance, Example 2 also takes into account the needs of multi-channel operation. More air holes 4 and / or equalizing holes 7 can be added to the disc cover or appropriate positions. For example, for the newly added annular distribution chamber, two symmetrically distributed equalizing holes 7 can be set on its top to ensure that there is no air pressure imbalance in the annular chamber when the liquid is quickly filled. At the same time, the original air holes 4 of each liquid guide groove 2 and liquid storage tank 3 are increased proportionally so that when the twelve channels are simultaneously filled with liquid, the air can be discharged out of the system in a timely and smooth manner. Through these improvements, the test disc of Example 2 can still maintain good liquid flow and distribution stability even when processing more test strips at the same time.

[0061] The method of use of this embodiment is basically the same as that of Example 1. The operator installs up to 12 test strips into each slot on the disc as needed, pours in an appropriate amount of liquid to be tested (the sample amount is appropriately increased according to the number of test strips), and then connects the test disc to the rotating seat for centrifugal distribution. Due to structural optimization, even if twelve test strips are supplied at the same time, the sample liquid can be distributed in place almost synchronously. The wetting, reaction and reading steps of each test strip are similar to the above process. Processing such a large number of test strips in a single test is very suitable for large-scale testing laboratories or occasions where rapid screening of multiple sample indicators is required. It should be noted that if the number of test strips is less than the maximum number of slots, for example, only some of the slots are used for testing, the test disc of this embodiment is also applicable. The liquid guide groove 2 and liquid storage groove 3 where no test strips are placed will not hinder the normal operation of other channels, and the excess sample liquid will eventually be collected through the overflow liquid collection groove 6, without affecting the overall test results.

[0062] Example 3

[0063] This embodiment proposes a test method based on the single-sample multi-item joint test method described in Example 1 or Example 2, which is suitable for batch test strip quantitative detection, especially for multi-parameter, high-throughput scenarios such as clinical urine analysis. This method combines the rotating liquid separation structure of the test disk with the diversion and liquid guide system to ensure that the sample liquid is evenly distributed in multiple test channels and output stably. Specifically, it includes the following steps:

[0064] Step 1, test paper preparation and installation: The operator first prepares the test strips to be tested, such as urine multi-parameter test strips. According to the detection requirements, take out the corresponding number of test strips (such as 6 or 12 strips) from the test paper package, and insert them into the test paper mounting slots 10 on the test disc respectively. The test paper must ensure that the reagent sensing end is facing the center of the disc body, facing the respective test liquid outlets 9. The test paper can be clamped by the slot body or an elastic positioning structure can be set to achieve stable fixation. In Example 1, the test disc is provided with 6 test paper mounting slots 10, which is suitable for medium batch detection; in Example 2, the test disc is provided with 12 test paper mounting slots 10, which is suitable for high-throughput detection.

[0065] Step 2: Sample Liquid Injection: Hold the test tray horizontally and inject the test liquid (e.g., a mixed urine sample) into the central test liquid chamber 1. The injection volume should be slightly larger than the total required volume (e.g., 0.2 mL per test strip, or approximately 2.5 mL for 12 strips). Injection should be done slowly to avoid bubbles or splashing.

[0066] Step three, rotation drive and centrifugal separation: install the test disc on the matching external rotating seat. Start the rotating device, and the test disc rotates around the central axis at the set speed. At this time, the test liquid in the accommodating chamber is thrown into multiple liquid guide grooves 2 in the radial direction under the action of centrifugal force. In Example 1, the liquid flows through 6 liquid guide grooves 2 and enters 6 liquid storage tanks 3 respectively; in Example 2, the liquid is diverted into 12 channels at the same time. Due to the cooperation of the diversion wall 11 and the flow limiting structure, the amount of liquid at the inlet of each liquid guide groove 2 is approximately the same, thereby ensuring that each liquid storage tank 3 is filled evenly.

[0067] Step 4: Filling of reservoir 3 and excess liquid overflow: Liquid is injected into reservoir 3 through liquid guide groove 2 and smoothly exhausted by air holes 4. As the liquid level in reservoir 3 rises, when the liquid reaches a preset height, the excess test liquid automatically flows through overflow port 5 into overflow liquid collection tank 6, which is located around the outer edge of the test disc. This process is accompanied by the buffering of air pressure by pressure equalization holes 7, maintaining a stable pressure differential between the inside and outside of the tank body, preventing liquid surges or vacuum blockages. This step ensures that each reservoir 3 reaches the same liquid level, and excess sample is collected and collected to avoid contamination or waste.

[0068] Step 5: Siphon Drainage and Quantitative Addition: After the rotation stabilizes, the drainage channel 8 activates the siphon mechanism. Because the reservoir 3 is located above the test liquid outlet 9 and the drainage channel 8 is designed as a curved, concave structure, the liquid flows spontaneously along the drainage channel 8, driven by the gravity difference of the liquid column and air pressure, into the test liquid outlet 9. At this point, the test liquid is dripped onto the sensing area of ​​each test strip at a well-controlled flow rate. The dripping position is stable and accurate, and each test strip receives an equal volume of test liquid, ensuring consistent reaction conditions.

[0069] Step 6: Reaction and Reading: After adding the liquid, continue rotating the test strip for approximately 10–20 seconds to allow the liquid to fully absorb and maintain uniform reaction conditions. Then, stop rotating and allow the test strip to rest for the specified reaction time. After the reaction is complete, read the test results of each test strip manually using colorimetry or a compatible reader.

[0070] In Example 1, six test strips were read; in Example 2, 12 test strips can be read simultaneously, significantly improving testing efficiency. Optional additional steps include automated reading and recycling: This method can also be combined with an automated reading module to implement digital processing such as color scale comparison and optical recognition. After the test is completed, the test strips are removed from the slot, or the entire test tray is removed for unified processing.

[0071] Through the above steps, Example 3 provides a reliable, efficient, and low-error test strip testing method that is suitable for both small laboratories performing sample-by-sample testing and large institutions performing multi-sample parallel analysis. Combined with the structural designs of Examples 1 and 2, this method has good adaptability and scalability.

[0072] In summary, each embodiment of the present invention achieves batch quantitative testing of test strips through centrifugal rotation, providing a test disc solution with a simple structure, high efficiency and reliability. It should be noted that the above embodiments are only used to illustrate the technical principles and beneficial effects of the present invention and do not limit the scope of protection of the present invention. Any equivalent replacement or modification made under the spirit and principles of the present invention shall be covered by the scope of protection of the present invention.

Claims

1. A test method for single-sample multi-item joint testing, wherein the test method is performed using a test disc connected to the top of an external rotating base, wherein the external rotating base is used to drive the test disc to rotate, characterized in that: The test tray is provided with: a test liquid accommodating cavity, a liquid guide groove, a liquid storage groove, an air hole, an overflow port, an overflow liquid collecting groove, a pressure equalizing hole, a drainage groove, a test liquid outlet and a test paper installation groove; The test liquid accommodating chamber is located in the test tray; the liquid guide groove is connected to the test liquid accommodating chamber at one end and to the liquid reservoir at the other end; the air hole is connected to the liquid reservoir; the overflow port is provided at the upper portion of the liquid reservoir and is connected to the overflow liquid collecting tank; the equalizing hole is provided on the overflow liquid collecting tank; the drainage groove is connected to the bottom of the liquid reservoir at one end and to the test liquid outlet at the other end; the test paper mounting groove is provided on the test tray; the test liquid outlet is provided on the test tray and is located above the test paper mounting groove; The test method comprises the following steps: Adding a test liquid into the test liquid receiving cavity of the test disc; The test disc is driven to rotate by the external rotating seat, so that the test liquid flows through the liquid guide groove into the liquid storage tank under the action of centrifugal force; When the liquid storage tank and the air hole are filled with the test liquid, the excess test liquid is discharged into the overflow liquid collection tank through the overflow port, and the air pressure is balanced through the pressure equalization hole; Under the siphon effect of the drainage groove, the test liquid in the liquid storage tank is led to the test liquid outlet; The test liquid is dripped onto the test paper in the test paper installation groove through the test liquid outlet, so that the test paper reacts with the test liquid.

2. The test method for single-sample multi-item joint detection according to claim 1, characterized in that: The test liquid accommodating cavity is a circular or polygonal groove located in the center of the test disc, and its side wall is provided with an opening connected to the liquid guiding groove.

3. The test method for single-sample multi-item joint detection according to claim 2, characterized in that: The drainage groove is a curved channel.

4. The test method for single-sample multi-item joint detection according to claim 1, characterized in that: A mounting portion is provided at the center of the bottom of the test disc. The mounting portion is mounted on the external rotating seat and is detachably connected to the external rotating seat.

5. The test method for single-sample multi-item joint detection according to claim 1, characterized in that: One or more groups of test paper fixing ribs are provided in the test paper installation groove, and the test paper fixing ribs are used to fix the test paper installed in the test paper installation groove.

6. The test method for single-sample multi-item joint detection according to claim 1, characterized in that: The test paper installation grooves are evenly distributed around the periphery of the test disc.

7. The test method for single-sample multi-item joint detection according to claim 1, characterized in that: An annular diversion wall is provided on the periphery of the test liquid accommodating chamber, and the diversion wall divides the test liquid accommodating chamber into a plurality of flow channels, each of which is connected to a corresponding liquid storage tank through a liquid guide groove.

8. The test method for single-sample multi-item joint detection according to claim 7, characterized in that: The overflow liquid collecting groove is an annular groove, which is arranged around the periphery of the test disc.

9. The method for single-sample multi-item joint detection according to claim 1, characterized in that: The drainage trough is arranged at a height higher than the liquid storage tank.

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